Freezing device for uniform forming of silk fibroin microspheres

Abstract

The freezing device comprises a peripheral supporting body, a rectangular cavity is formed in the peripheral supporting body, the lower end of the peripheral supporting body is fixedly connected with a bottom heat conduction material, the lower end of the bottom heat conduction material is provided with a cold source, the upper end of the peripheral supporting body is provided with a sealing cover, and the sealing cover is fixedly connected with the rectangular cavity. A plurality of penetrating openings are formed in the bottom heat conduction material, a plurality of heat conduction rods are arranged in the peripheral supporting body, the lower ends of the heat conduction rods penetrate through the corresponding penetrating openings, a plurality of round openings are formed in the sealing cover, and the upper ends of the heat conduction rods penetrate through the round openings. According to the device, by replacing the number and the material of the heat conduction rods, silk fibroin microspheres with different particle sizes can be regulated and controlled, heat transfer is more uniform, a silk fibroin solution in the cavity forms relatively uniform ice crystals, meanwhile, a cold source and the bottom heat conduction material can be rapidly fixed, and the heat conduction efficiency is improved. And the condition that the bottom heat conduction material is separated from the cold source due to accidental touch is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of the formation and preparation of silk fibroin microspheres, in particular to a freezing device for the uniform formation of silk fibroin microspheres. Background Art

[0002] Silk fibroin microspheres are micron-sized spherical materials made of silk fibroin with good biocompatibility, having unique biodegradability and biological activity. The preparation technology usually involves forming microspheres by methods such as emulsification, freeze self-assembly, spray drying or electrospray of silk fibroin solution, and then solidifying and forming them by freeze drying or other cross-linking technologies.

[0003] Silk fibroin microspheres have a wide range of uses, including as drug delivery systems, cell carriers in tissue engineering, and applications in the cosmetics and food industries. They can control the release of drugs, promote cell growth and differentiation, and at the same time have good mechanical properties and stability. However, in the prior art, when preparing and applying silk fibroin microspheres, the particle size and distribution of the microspheres cannot be accurately controlled. Therefore, it is necessary to design a freezing device for the uniform formation of silk fibroin microspheres to solve the above problems. Summary of the Utility Model

[0004] The purpose of the utility model is to solve the deficiencies existing in the prior art, and to propose a freezing device for the uniform formation of silk fibroin microspheres. By replacing the quantity and material of the heat conduction rods, the regulation of silk fibroin microspheres with different particle sizes can be realized, and the heat transfer is more uniform, so that the silk fibroin solution in the cavity forms relatively uniform ice crystals. At the same time, the cold source can be quickly fixed to the bottom heat conduction material, avoiding the situation that the bottom heat conduction material is separated from the cold source due to accidental collision.

[0005] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0006] A freezing device for the uniform formation of silk fibroin microspheres, including an outer support body, a rectangular cavity is arranged inside the outer support body, a bottom heat conduction material is fixedly connected to the lower end of the outer support body, a cold source is arranged at the lower end of the bottom heat conduction material, a sealing cover is arranged at the upper end of the outer support body, a plurality of through holes are arranged on the bottom heat conduction material, a plurality of heat conduction rods are arranged inside the outer support body, the lower ends of the plurality of heat conduction rods all penetrate through the corresponding through holes, and a plurality of circular holes are arranged on the sealing cover, and the upper ends of the plurality of heat conduction rods all penetrate through the circular holes.

[0007] Preferably, the bottom heat-conducting material is connected to the cold source through a connecting component. The connecting component includes a mounting block disposed between the bottom heat-conducting material and the cold source. The upper ends of the two mounting blocks located below are fixedly connected with threaded columns. Through holes are provided on the two mounting blocks located above. The two threaded columns penetrate through the through holes, and nuts are threadedly connected to the two threaded columns.

[0008] Preferably, threaded sleeves are embedded in multiple circular openings. The upper ends of multiple heat-conducting rods penetrate through the threaded sleeves. Threaded layers are provided on the outer walls of the multiple threaded sleeves, and threaded caps are threadedly connected to the multiple threaded sleeves.

[0009] Preferably, the number of both the circular openings and the through holes is nine.

[0010] Preferably, the cold source includes a storage box, and liquid nitrogen or ice cubes are stored in the storage box.

[0011] Preferably, both the bottom heat-conducting material and multiple heat-conducting rods are made of iron materials.

[0012] Compared with the prior art, the advantages of the present device are as follows:

[0013] 1. Compared with the prior art, the temperature of the cold source is evenly conducted to the inside of the mold cavity through the heat-conducting material and the heat-conducting rod body. The more heat-conducting rod bodies are provided, the more uniform the heat transfer received by the silk fibroin solution is, so that relatively uniform ice crystals are formed in the cavity of the silk fibroin solution, and the silk fibroin in the silk fibroin solution self-assembles and folds into microspheres with uniform particle sizes;

[0014] 2. Compared with the prior art, by setting different numbers of heat-conducting rod bodies in the mold cavity or changing the materials of the heat-conducting rod bodies, the regulation of silk fibroin microspheres with different particle sizes can be realized;

[0015] 3. Compared with the prior art, through the setting of the connecting component, the cold source and the bottom heat-conducting material can be made not easy to separate. At the same time, through the setting of the threaded sleeve and the threaded cap, the sealing performance of the sealing cap can be ensured after the heat-conducting rod is drawn out. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic structural diagram of a freezing device for uniformly forming silk fibroin microspheres proposed by the present utility model;

[0017] Figure 2 is a schematic internal structure diagram of the peripheral support body;

[0018] Figure 3 is Figure 1 the enlarged structural diagram at A in

[0019] Figure 4Schematic structural diagram of the sealing cover;

[0020] Figure 5 Schematic structural diagram of the bottom heat-conducting material.

[0021] In the figure: 1 peripheral support body, 2 sealing cover, 3 rectangular cavity, 4 bottom heat-conducting material, 5 cold source, 6 heat-conducting rod, 7 mounting block, 8 threaded post, 9 nut, 10 threaded sleeve, 11 threaded cover, 12 through hole. Specific implementation manner

[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0023] Referring to Figures 1 - 5 , a freezing device for the uniform molding of silk fibroin microspheres, including a peripheral support body 1, a rectangular cavity 3 is provided inside the peripheral support body 1, the lower end of the peripheral support body 1 is fixedly connected with a bottom heat-conducting material 4, a cold source 5 is provided at the lower end of the bottom heat-conducting material 4, the cold source 5 includes a storage box, and liquid nitrogen or ice cubes are stored in the storage box. A sealing cover 2 is provided at the upper end of the peripheral support body 1, a plurality of through holes 12 are provided on the bottom heat-conducting material 4, a plurality of heat-conducting rods 6 are provided inside the peripheral support body 1, the lower ends of the plurality of heat-conducting rods 6 all penetrate through the corresponding through holes 12, a plurality of round holes are provided on the sealing cover 2, the upper ends of the plurality of heat-conducting rods 6 all penetrate through the round holes, and the number of the round holes and the through holes 12 is nine.

[0024] Among them, the bottom heat-conducting material 4 and the cold source 5 are connected by a connecting component. The connecting component includes a mounting block 7 arranged between the bottom heat-conducting material 4 and the cold source 5. The upper ends of the two mounting blocks 7 located below are fixedly connected with threaded posts 8, through holes are provided on the two mounting blocks 7 located above, the two threaded posts 8 all penetrate through the through holes, and nuts 9 are threadedly connected to the two threaded posts 8.

[0025] Among them, threaded sleeves 10 are embedded in a plurality of round holes, the upper ends of the plurality of heat-conducting rods 6 all penetrate through the threaded sleeves 10, threaded layers are provided on the outer walls of the plurality of threaded sleeves 10, and threaded covers 11 are threadedly connected to the plurality of threaded sleeves 10.

[0026] Among them, the cold source 5 includes a storage box, and liquid nitrogen or ice cubes are stored in the storage box.

[0027] The functional principle of the present utility model can be elaborated through the following operating method: Dissolve a silk fibroin solution with a concentration of 1% to 15%, add 0.1% to 5% ethanol to the silk fibroin solution, and gently stir and mix evenly. Pour the evenly mixed silk fibroin mixed solution into the rectangular cavity 3 of the mold, cover the sealing cover 2 with several heat conduction rods 6 tightly, and both ends of the heat conduction rod 6 are tightly connected and fixed to the circular opening of the sealing cover 2 and the through hole of the bottom heat conduction material 4 respectively; contact the bottom heat conduction material 4 with the cold source 5, so that the threaded column 8 penetrates through the through port, and then thread the nut 9 onto the threaded column 8, so that the cold source 5 is fixed to the bottom heat conduction material 4; the cold source 5 can be liquid nitrogen, ice cubes, etc., or the whole mold can be erected and placed in a -20°C refrigerator / freezer. After the silk fibroin solution in the rectangular cavity 3 is completely frozen and solidified, place the whole mold in a -20°C refrigerator / freezer and freeze for 5 to 24 hours; thaw the silk fibroin frozen body in the rectangular cavity 3 to obtain a milky white suspension; wash the obtained white suspension and centrifuge to remove β-sheet inducing agents such as ethanol; freeze-dry the microspheres obtained by washing and centrifuging to obtain dry silk fibroin microspheres.

[0028] The above is only the preferred specific implementation mode of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution of the present utility model and its inventive concept, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present utility model.

Claims

1. A freezing device for the uniform molding of silk fibroin microspheres, comprising an outer support (1), characterized in that: A rectangular cavity (3) is provided inside the peripheral support body (1). A bottom heat-conducting material (4) is fixedly connected to the lower end of the peripheral support body (1). A cold source (5) is provided at the lower end of the bottom heat-conducting material (4). A sealing cover (2) is provided at the upper end of the peripheral support body (1). A plurality of through holes (12) are provided on the bottom heat-conducting material (4). A plurality of heat-conducting rods (6) are provided inside the peripheral support body (1). The lower ends of the plurality of heat-conducting rods (6) all penetrate through the corresponding through holes (12). A plurality of round holes are provided on the sealing cover (2). The upper ends of the plurality of heat-conducting rods (6) all penetrate through the round holes.

2. The freezing device for uniform forming of silk fibroin microspheres according to claim 1, characterized in that: The bottom heat-conducting material (4) and the cold source (5) are connected through a connecting component. The connecting component includes a mounting block (7) arranged between the bottom heat-conducting material (4) and the cold source (5). The upper ends of the two mounting blocks (7) located below are both fixedly connected with threaded columns (8). Through holes are provided on the two mounting blocks (7) located above. The two threaded columns (8) both penetrate through the through holes. Nuts (9) are threadedly connected to the two threaded columns (8).

3. The freezing device for uniform forming of silk fibroin microspheres according to claim 1, wherein: Threaded sleeves (10) are embedded in the plurality of round holes. The upper ends of the plurality of heat-conducting rods (6) all penetrate through the threaded sleeves (10). Threaded layers are provided on the outer walls of the plurality of threaded sleeves (10). Threaded caps (11) are threadedly connected to the plurality of threaded sleeves (10).

4. A freezing device for uniform forming of silk fibroin microspheres according to claim 1, characterized in that: The number of the round holes and the through holes (12) is nine.

5. A freezing device for uniform shaping of silk fibroin microspheres according to claim 1, characterized in that: The cold source (5) includes a storage box, and liquid nitrogen or ice cubes are stored in the storage box.

6. The cryogenic device for uniform forming of silk fibroin microspheres according to claim 1, characterized in that: The bottom heat-conducting material (4) and the plurality of heat-conducting rods (6) are both made of iron materials.

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