Modularized structure of fluorescent microscopic optical system
By designing the fluorescence microscopic optical system into a modular structure, independent disassembly and multi-dimensional adjustment of each module is achieved, which solves the problems of high maintenance costs and insufficient system stability in the prior art, and improves the stability and imaging quality of the system.
Patent Information
- Application Number
- CN202422066965.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-26
- Publication Date
- 2025-07-08
- Estimated Expiration
- 2034-08-26
AI Technical Summary
During the maintenance and repair process, existing fluorescence microscopy optical systems require the entire large plate to be transported back to the original factory or on-site for maintenance, resulting in high time and cost, and system stability and imaging quality are greatly affected by failures of a single module.
The fluorescence microscopic optical system is designed as a modular structure, including a support body, an objective lens module, a camera module and a focus alignment module. Each module is independently detachable and equipped with multi-dimensional position adjustment function, and precise adjustment is achieved through a micro-adjustment mechanism.
Improves system stability and imaging quality, reduces system downtime caused by failure of individual modules, simplifies maintenance and upgrade processes, and reduces transportation and maintenance costs.
Smart Images
Figure CN223078542U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of fluorescence microscopy optical systems, and particularly to a modular structure of a fluorescence microscopy optical system. Background Art
[0002] A fluorescence microscopy optical system is a system that uses light of a certain wavelength to excite fluorescent substances under a fluorescence microscope objective, and the generated fluorescence passes through the fluorescence microscope objective and is received by the human eye or a camera. During the production and manufacturing process of the fluorescence microscopy optical system, each component that constitutes a complex optical path system is usually installed on a large flat plate. These components are distributed at different positions on the flat plate and are adjusted on the flat plate. After the system is adjusted and qualified, it is inspected and shipped as a whole. However, in later maintenance, it is necessary to transport the entire large flat plate containing the optical system back to the original factory for repair, or the maintenance personnel go to the site for maintenance, which takes a long time and incurs high costs. Content of the Utility Model
[0003] Purpose of the Utility Model: The purpose of the utility model is to provide a modular structure of a fluorescence microscopy optical system that is modular and detachable.
[0004] Technical Solution: To achieve the above purpose, a modular structure of a fluorescence microscopy optical system described in the utility model includes a support body. The support body is a cavity structure, and a dichroic mirror is arranged inside. An objective lens module, a camera module, and a focal plane alignment module are assembled on the support body. The objective lens module, the camera module, and the focal plane alignment module are all independent components, and together with the dichroic mirror, they form an optical path for observing fluorescent substances inside the cavity of the support body.
[0005] Among them, a first through hole penetrating up and down is arranged on the support body. The upper end of the first through hole is installed with the focal plane alignment module, and the dichroic mirror is located inside the first through hole. A second through hole for installing the objective lens module is arranged on one side of the first through hole. The objective lens module includes a microscope objective lens located below the dichroic mirror. The camera module is installed on the side wall of the support body, and a tube lens is located between the camera module and the dichroic mirror. The camera module is used to capture the fluorescent light signal passing through the microscope objective lens, the dichroic mirror, and the tube lens.
[0006] Among them, the objective lens module includes a linear displacement stage. The linear displacement stage includes two sliding plates that move relatively up and down in the vertical direction. One of the sliding plates is fixed inside the second through hole, and the lower end of the other sliding plate is installed with the microscope objective lens through a first micro-adjustment mechanism.
[0007] Among them, the first micro-adjustment mechanism includes an inclined adjustment plate fixed to the lower end of another slide plate. The inclined adjustment plate is rotatably installed with an objective lens adapter through a first pin. Below the objective lens adapter, the inclined adjustment plate is provided with a first boss extending outward. A adjusting screw is provided on the first boss and is in threaded cooperation. The upper end of the adjusting screw abuts against the lower surface of the objective lens adapter, thereby adjusting the inclination angle of the objective lens adapter relative to the horizontal plane with the first pin as the central axis; the objective lens adapter is provided with a central through hole in the vertical direction, and the microscopic objective lens is fixed in the central through hole through an objective lens mounting seat.
[0008] Among them, the objective lens mounting seat and the fixed end of the objective lens adapter are provided with a second boss extending outward. A through hole is provided on the second boss, and a threaded hole is provided on the objective lens adapter at the corresponding position of the through hole, so as to fix the objective lens mounting seat in the central through hole of the objective lens adapter by using a first fixing screw; outside the first fixing screw, an inclined adjustment ring is sleeved between the contact surfaces of the second boss and the objective lens adapter. One surface of the inclined adjustment ring is a plane, and the other is a bevel surface with a wedge angle. By rotating the inclined adjustment ring, the relative inclination relationship between the objective lens mounting seat and the objective lens adapter is adjusted, and further the inclination angle of the microscopic objective lens relative to the horizontal plane is adjusted.
[0009] Among them, the camera module includes a camera, and the front end of the camera is installed on the support body through a second micro-adjustment mechanism.
[0010] Among them, the second micro-adjustment mechanism includes a camera interface fixing plate, a camera translation adjustment plate, a camera tilt adjustment plate, a camera tilt adjustment base, and a camera focusing base, all of which have a light-passing through hole structure in the middle; the camera interface fixing plate, the camera translation adjustment plate, and the camera tilt adjustment plate are guided by a second pin to perform translation adjustment of the camera in the horizontal and vertical directions; the surfaces of the camera tilt adjustment plate in contact with the camera tilt adjustment base are respectively an arc convex surface and an arc concave surface with the same curvature size, and the inclination angle of the camera in the horizontal and pitch directions is adjusted by relative sliding of the arc surfaces; both the camera tilt adjustment base and the camera focusing base are provided with tubular parts, and the focusing of the camera is performed by the length of the tubular parts sleeved together.
[0011] Among them, the camera focusing base is installed on the support body, and the camera is fixed on the camera interface fixing plate.
[0012] Among them, the structure in which the camera interface fixing plate, the camera translation adjustment plate, and the camera tilt adjustment plate are guided by a second pin is as follows: there are second pins on the front and rear surfaces of the camera translation adjustment plate, and the camera interface fixing plate and the camera tilt adjustment plate are respectively provided with kidney slots on the contact surfaces with the camera translation adjustment plate, which are matched with the corresponding second pins. Among them, the length direction of the kidney slot on the camera interface fixing plate is perpendicular to the length direction of the kidney slot on the camera tilt adjustment plate.
[0013] Beneficial effects: The utility model has the following advantages: 1. The components involved in the fluorescence microscopy optical system of the utility model are integrated into multiple structurally compact and independent modules. In practical applications, it is not only convenient for management and transportation, but also enables each module to be replaced or upgraded separately, improving the overall stability of the system and avoiding long-term downtime of the system caused by the failure of a single module.
[0014] 2. The objective lens module and the camera module both have multi-dimensional position adjustment functions, ensuring that the fluorescent substance is within the clear imaging range of the objective lens module and the camera module, improving the imaging quality. At the same time, when observing the fluorescent substance, it can also reduce the adjustment dependence on other modules, improving the independent operability and adjustment efficiency of a single module. Description of the drawings
[0015] Figure 1 It is a structural schematic diagram of the utility model;
[0016] Figure 2 It is a structural schematic diagram of the objective lens module;
[0017] Figure 3 It is a structural schematic diagram of the connection structure between the tilt adjustment and the objective lens adapter;
[0018] Figure 4 It is a structural schematic diagram of the tilt adjustment ring installation;
[0019] Figure 5 It is a structural schematic diagram of the camera module;
[0020] Figure 6 It is a sectional structural schematic diagram of the camera module. Detailed implementation manners
[0021] The technical solutions of the utility model will be described in detail below in conjunction with the embodiments and the drawings.
[0022] As Figure 1 shown, the modular structure of the fluorescence microscopy optical system of the utility model includes a support body 1, which is designed as a cavity structure and internally provided with a dichroic mirror and a tube lens. On the support body 1, an objective lens module 2, a camera module 3 and a focal plane alignment module 4 are assembled, and these modules together form an optical path for observing fluorescent substances inside the cavity of the support body 1.
[0023] The focal plane alignment module 4 includes an illumination system and a reflector. The illumination system emits light of a certain wavelength, which passes through the reflector, the dichroic mirror and the objective lens module 2 to excite the fluorescent substance located below the objective lens module 2. After the fluorescent substance is illuminated, the light passes through the objective lens module 2, the dichroic mirror and the tube lens and is imaged on the camera module 3.
[0024] The support body 1, the objective lens module 2, the camera module 3, and the focal plane alignment module 4 are all independent modules, which are convenient for disassembly and assembly, enabling each module to be replaced or upgraded separately. Among them, the objective lens module 2 and the camera module 3 have built-in position adjustment functions to ensure that the fluorescent substance is within the clear imaging range of the objective lens module 2 and the camera module 3, thereby improving the imaging quality.
[0025] The specific structure is as follows: The support body 1 is a square cavity structure, and a first through hole penetrating up and down is provided in the middle area. The upper end of the first through hole is equipped with the focal plane alignment module 4, and the reflector of the focal plane alignment module 4 is located above the dichroic mirror. On one side of the first through hole, there is a second through hole for installing the objective lens module 2, and the microscopic objective lens 2.1 of the objective lens module 2 is located below the dichroic mirror. Two groups of camera modules 3 are installed on adjacent side walls of the support body 1. The tube lens is located between the camera module 3 and the dichroic mirror. The camera module 3 is used to capture the fluorescent light signals passing through the microscopic objective lens 2.1, the dichroic mirror, and the tube lens.
[0026] As Figure 2 shown, the objective lens module 2 includes a linear displacement stage 2.2. The linear displacement stage 2.2 includes two sliding plates that move relatively up and down in the vertical direction. Among them, the left sliding plate is provided with a first threaded hole. When the linear displacement stage 2.2 is located in the second through hole, a third through hole is provided on the support body 1 at the position corresponding to the threaded hole of the left sliding plate. That is, a screw is passed through the third through hole and fixed at the first threaded hole, thereby fixing the objective lens module 2 in the second through hole.
[0027] The lower end of the right sliding plate of the linear displacement stage 2.2 is fixed with an inclination adjustment plate 2.3. The inclination adjustment plate 2.3 realizes translation in the vertical direction under the drive of the linear displacement stage 2.2. As Figure 3 shown, the inclination adjustment plate 2.3 installs the objective lens adapter 2.4 through a first pin 2.9. Relative to the inclination adjustment plate 2.3, the objective lens adapter 2.4 can rotate around the first pin 2.9 as the central axis. Below the objective lens adapter 2.4, the inclination adjustment plate 2.3 is provided with a first boss extending outward. The first boss is provided with a second threaded hole. By adjusting the screwing in and out of the adjustment screw 2.7 in the second threaded hole, the rotation angle of the objective lens adapter 2.4 around the first pin 2.9 is adjusted, realizing the adjustment of the inclination angle of the objective lens adapter 2.4 relative to the horizontal plane (Y-axis direction), as Figure 2 shown by the rotation arrow 1 in
[0028] As Figure 4As shown in the figure, a central through hole for placing the microscopic objective lens 2.1 is provided in the vertical direction at the center of the objective lens adapter bracket 2.4, and the microscopic objective lens 2.1 is installed in the objective lens mounting base 2.5. The upper end of the objective lens mounting base 2.5 is provided with a second boss extending outward, and a fourth through hole is provided on the second boss. The objective lens adapter bracket 2.4 is provided with a third threaded hole at the corresponding position of the fourth through hole, so that the objective lens mounting base 2.5 is fixed in the central through hole of the objective lens adapter bracket 2.4 by using the first fixing screw 2.8. An inclination adjustment ring 2.6 is sleeved between the outer ring of the first fixing screw 2.8 and the contact surface between the second boss and the objective lens adapter bracket 2.4. One surface of the inclination adjustment ring 2.6 is a flat surface, and the other is a 10′ wedge angle inclined surface (relative to the contact surface of the boss or the objective lens adapter bracket 2.4). By rotating the inclination adjustment ring 2.6, the relative inclination relationship between the objective lens mounting base 2.5 and the objective lens adapter bracket 2.4 is adjusted, and the inclination angle of the microscopic objective lens 2.1 relative to the horizontal plane (X-axis direction) is adjusted, as Figure 2 shown by the rotation arrow 2 in the figure. During the adjustment process, first loosen the first fixing screw 2.8, turn the inclination adjustment ring 2.6 by the required angle, and then tighten the first fixing screw 2.8, so as to fix the objective lens mounting base 2.5, the objective lens adapter bracket 2.4, and the inclination adjustment ring 2.6 again.
[0029] Through the adjustment of the inclination adjustment ring 2.6 of the objective lens module 2, the inclination angle of the microscopic objective lens 2.1 in the X-axis direction is adjusted, and the adjustment accuracy can reach 10″. Through the adjustment of the above-mentioned adjustment screw 2.7, the inclination angle of the microscopic objective lens 2.1 in the Y-axis direction is adjusted. The pitch of the adjustment screw 2.7 is 0.35 mm, and the adjustment accuracy can reach 40″.
[0030] As Figure 5 and 6 shown in the figure, the camera module 3 includes a camera 3.1. The front end (light receiving end) of the camera 3.1 is successively provided with a camera interface fixing plate 3.2, a camera translation adjustment plate 3.3, a camera inclination adjustment plate 3.4, a camera inclination adjustment base 3.5, and a camera focusing base 3.6, all of which have a through hole structure in the middle that does not block light; among them, the camera focusing base 3.6 is installed on the support body 1, and the camera 3.1 is fixed on the camera interface fixing plate 3.2.
[0031] On the front and back surfaces of the camera translation adjustment plate 3.3, there are two groups of second pins 3.8 each. On the contact surfaces of the camera interface fixing plate 3.2 and the camera tilt adjustment plate 3.4 with the camera translation adjustment plate 3.3, there are two groups of kidney slots that cooperate with the corresponding second pins 3.8 (the kidney slot of the camera interface fixing plate 3.2 can be horizontal, and the camera tilt adjustment plate 3.4 can be vertical). The camera interface fixing plate 3.2, the camera translation adjustment plate 3.3, and the camera tilt adjustment plate 3.4 are guided by the second pins 3.8, enabling relative translation adjustment in the vertical direction between the camera interface fixing plate 3.2 and the camera tilt adjustment plate 3.4 relative to the camera translation adjustment plate 3.3, thereby achieving the translation adjustment of the camera 3.1 in the horizontal and vertical directions. After the adjustment is completed, the camera interface fixing plate 3.2, the camera translation adjustment plate 3.3, and the camera tilt adjustment plate 3.4 are locked by the second fixing screw 3.9 passing through them (the camera interface fixing plate 3.2 is provided with a fourth threaded hole, and the camera translation adjustment plate 3.3 and the camera tilt adjustment plate 3.4 are provided with fifth through holes at the corresponding positions of the fourth threaded hole. The second fixing screw 3.9 passes through the fifth through hole and is fixed on the fourth threaded hole of the camera interface fixing plate 3.2). During the installation and debugging process, the debugging tooling holes on the sides of these three components are adjusted by set screws, and the adjustment accuracy can reach 5um.
[0032] The surfaces of the camera tilt adjustment plate 3.4 in contact with the camera tilt adjustment base 3.5 are respectively a convex arc surface and a concave arc surface with the same curvature. The two are adjusted for the tilt angle by relative sliding on the arc surface, achieving the tilt adjustment of the camera 3.1 in the horizontal and pitch directions. After the adjustment is completed, the camera tilt adjustment plate 3.4 and the camera tilt adjustment base 3.5 are locked by the third fixing screw 3.10 (the camera tilt adjustment plate 3.4 is provided with a fifth threaded hole, and the camera tilt adjustment base 3.5 is provided with a sixth through hole at the corresponding position of the fifth threaded hole. The third fixing screw 3.10 passes through the sixth through hole and is fixed on the fifth threaded hole of the camera tilt adjustment base 3.5). During the installation and debugging process, the debugging tooling holes on the sides of these two components are adjusted by set screws, and the adjustment accuracy can reach 30″.
[0033] Both the camera tilt adjustment base 3.5 and the camera focusing base 3.6 have tubular parts and are sleeved together through the tubular parts. The tubular part of the camera focusing base 3.6 is located inside and is provided with a sixth threaded hole. The tubular part of the camera tilt adjustment base 3.5 is located outside and is provided with a kidney hole at the corresponding position of the sixth threaded hole. The guiding locking screw 3.6 guides and locks the camera adjustment base 3.5 and the camera focusing base 3.6 through the kidney hole. The two can move relative to each other axially to achieve the focusing of the camera 3.1. During the installation and debugging process, the debugging tooling holes on the sides of these two components are adjusted by set screws, and the adjustment accuracy can reach 5um.
Claims
1. A modular structure of a fluorescence microscopy optical system, characterized in that: It includes a support body (1) which is a cavity structure with a dichroic mirror and a tube lens inside. An objective lens module (2), a camera module (3) and a focal plane alignment module (4) are assembled on the support body (1). The objective lens module (2), the camera module (3) and the focal plane alignment module (4) are all independent components, and together with the dichroic mirror and the tube lens inside the cavity of the support body (1), they form an optical path for observing fluorescent substances.
2. The modular structure of the fluorescence microscopy optical system according to claim 1, wherein: There is a first through hole penetrating up and down on the support body (1). The focal plane alignment module (4) is installed at the upper end of the first through hole, and the dichroic mirror is located inside the first through hole. A second through hole for installing the objective lens module (2) is provided on one side of the first through hole. The objective lens module (2) includes a microscopic objective lens (2.1) located below the dichroic mirror. The camera module (3) is installed on the side wall of the support body (1), and the tube lens is located between the camera module (3) and the dichroic mirror. The camera module (3) is used to capture the fluorescent light signal passing through the microscopic objective lens (2.1), the dichroic mirror and the tube lens.
3. The modular structure of the fluorescence microscopy optical system according to claim 2, characterized in that: The objective lens module (2) includes a linear displacement stage (2.2). The linear displacement stage (2.2) includes two sliding plates that move relatively up and down in the vertical direction. One sliding plate is fixed inside the second through hole, and the lower end of the other sliding plate is installed with the microscopic objective lens (2.1) through a first micro-adjustment mechanism.
4. The modular structure of the fluorescence microscopic optical system according to claim 3, wherein: The first micro-adjustment mechanism includes an inclined adjustment plate (2.3) fixed at the lower end of the other sliding plate. The inclined adjustment plate (2.3) is rotatably installed with an objective lens adapter (2.4) through a first pin (2.9). Below the objective lens adapter (2.4), the inclined adjustment plate (2.3) is provided with a first boss extending outward. A adjusting screw (2.7) is provided on the first boss through threaded cooperation. The upper end of the adjusting screw (2.7) abuts against the lower surface of the objective lens adapter (2.4), thereby adjusting the inclination angle of the objective lens adapter (2.4) relative to the horizontal plane with the first pin (2.9) as the central axis; the objective lens adapter (2.4) is provided with a central through hole in the vertical direction, and the microscopic objective lens (2.1) is fixed inside the central through hole through an objective lens mounting seat (2.5).
5. The modular structure of the fluorescence microscopy optical system according to claim 4, wherein: The objective lens mounting seat (2.5) and the fixed end of the objective lens adapter (2.4) are provided with a second boss extending outward. The second boss is provided with a through hole, and the objective lens adapter (2.4) is provided with a threaded hole at the corresponding position of the through hole, so as to fix the objective lens mounting seat (2.5) inside the central through hole of the objective lens adapter (2.4) by using a first fixing screw (2.8); outside the first fixing screw (2.8), an inclined adjustment ring (2.6) is sleeved between the contact surfaces of the second boss and the objective lens adapter (2.4). One surface of the inclined adjustment ring (2.6) is a plane, and the other is an inclined surface with a wedge angle. By rotating the inclined adjustment ring (2.6), the relative inclination relationship between the objective lens mounting seat (2.5) and the objective lens adapter (2.4) is adjusted, and further the inclination angle of the microscopic objective lens (2.1) relative to the horizontal plane is adjusted.
6. The modular structure of the fluorescence microscopy optical system according to claim 1, characterized in that: The camera module (3) includes a camera (3.1), and the front end of the camera (3.1) is mounted on the support body (1) through a second fine adjustment mechanism.
7. The modular structure of the fluorescence microscopy optical system according to claim 6, characterized in that: The second fine adjustment mechanism includes a camera interface fixing plate (3.2), a camera translation adjustment plate (3.3), a camera tilt adjustment plate (3.4), a camera tilt adjustment base (3.5), and a camera focusing base (3.6), all of which have a light-passing through hole structure in the middle without light occlusion; the camera interface fixing plate (3.2), the camera translation adjustment plate (3.3), and the camera tilt adjustment plate (3.4) are guided by a second pin (3.8) to adjust the translation of the camera (3.1) in the horizontal and vertical directions; the surfaces of the camera tilt adjustment plate (3.4) in contact with the camera tilt adjustment base (3.5) are respectively an arc convex surface and an arc concave surface with the same curvature size, and the tilt angle of the camera (3.1) in the horizontal and pitch directions is adjusted by the relative sliding of the arc surfaces; both the camera tilt adjustment base (3.5) and the camera focusing base (3.6) are provided with tubular parts, and the focusing of the camera (3.1) is adjusted by the length of the tubular parts sleeved together.
8. The modular structure of the fluorescence microscopic optical system according to claim 7, characterized in that: The camera focusing base (3.6) is mounted on the support body (1), and the camera (3.1) is fixed on the camera interface fixing plate (3.2).
9. The modular structure of the fluorescence microscopy optical system according to claim 7, wherein: The structure in which the camera interface fixing plate (3.2), the camera translation adjustment plate (3.3), and the camera tilt adjustment plate (3.4) are guided by the second pin (3.8) is as follows: there are second pins (3.8) on the front and back surfaces of the camera translation adjustment plate (3.3), and kidney-shaped slots matching the corresponding second pins (3.8) are provided on the contact surfaces of the camera interface fixing plate (3.2) and the camera tilt adjustment plate (3.4) with the camera translation adjustment plate (3.3). Among them, the length direction of the kidney-shaped slot on the camera interface fixing plate (3.2) is perpendicular to the length direction of the kidney-shaped slot on the camera tilt adjustment plate (3.4).