Brain tissue low-temperature storage device

Abstract

A brain tissue low-temperature storage device comprises a containing box, at least six containing grooves are formed in the upper end of the containing box, at least six storage assemblies are arranged in the containing grooves, each storage assembly comprises a lower cover plate and a protective bottle body, the lower cover plates are movably connected to the interiors of the containing grooves, and the protective bottle bodies are arranged in the containing grooves. The protective bottle body is in threaded connection with the interior of the lower cover plate, a storage groove is formed in the lower cover plate, and the protective bottle body is movably connected to the outer side of the storage groove. By arranging the storage assembly, the protective bottle body is rotated, the lower end of the protective bottle body can be screwed into the lower cover plate, so that a closed space is formed, external pollutants are prevented from polluting brain tissues, protection of the brain tissues is facilitated, operation is simple, the brain tissues are prevented from being damaged, and in the process that the protective bottle body is screwed out of or screwed into the lower cover plate, the protective bottle body is prevented from being damaged. The protective bottle body is not in contact with the brain tissue, so that the brain tissue is prevented from being damaged by the protective bottle body, and the brain tissue is more comprehensively protected.

Description

Technical Field

[0001] The utility model relates to the technical field of brain tissue experiments, in particular to a low-temperature storage device for brain tissue. Background Technique

[0002] Experiments on rodents to open the skull and extract brain tissue are usually called "brain tissue sampling" or "brain tissue extraction" experiments. The purpose of these experiments is to obtain brain tissue samples of rodents for further biological, neurological or pathological research. Rodent brain research is an important part of neuroscience, providing a platform for in-depth exploration of complex issues such as neuroelectrophysiology, neuronal connections, neural plasticity, and neurodegenerative diseases. These studies help to reveal the microscopic details of the basic biology of the brain, provide key scientific evidence for understanding the functions of the human nervous system and disease mechanisms, and promote the frontier progress of neuroscience. For example, researchers may conduct such experiments to study the brain structure and function, or observe the changes in brain tissue in disease models. In some cases, such experiments are also related to the "brain slicing" technology in the field of neuroscience, that is, to obtain thin slices of specific brain regions through precise cutting for more detailed analysis.

[0003] In the prior art, there is a lack of a device for preserving brain tissue. Currently, in medical experiments, the brain tissue is usually wrapped on the outside with tin foil and then placed in a medical refrigerator for storage. However, this storage method has a poor protective effect on the brain tissue. If the brain tissue is wrapped and stored with tin foil, when the brain tissue needs to be taken out, improper operation may cause damage to the brain tissue, which is not convenient for taking out the brain tissue. Moreover, the tin foil has a relatively thin material, and when wrapped with tin foil, its heat preservation effect is poor, and the temperature drops quickly after being taken out, which is not conducive to the low-temperature protection of the brain tissue. Content of the Utility Model

[0004] To solve the above technical problems, a low-temperature storage device for brain tissue is provided. This technical solution solves the problems of poor protective effect on the brain tissue, inconvenience in taking out the brain tissue, and poor heat preservation effect.

[0005] To achieve the above purposes, the technical solution adopted by the utility model is as follows:

[0006] A low-temperature storage device for brain tissue, including a placement box. At least six groups of placement grooves are opened at the upper end of the placement box. At least six groups of storage components are arranged inside the placement grooves. The storage components include a lower cover plate and a protective bottle body. The lower cover plate is movably connected inside the placement groove. The protective bottle body is threadedly connected inside the lower cover plate. A storage groove is arranged inside the lower cover plate. The protective bottle body is movably connected to the outside of the storage groove. Two wedge-shaped grooves are opened on the outside of the lower cover plate. Two wedge-shaped blocks adapted to the wedge-shaped grooves are slidably connected to the inner wall of the placement groove.

[0007] Preferably, a guide rod is fixedly connected to the outside of the wedge block. The guide rod is slidably connected to the inside of the placement box. A limiting spring is sleeved on the outside of the guide rod. Two ends of the limiting spring are fixedly connected to the wedge block and the placement box respectively.

[0008] Preferably, a substrate is slidably connected to the inside of the placement groove. A buffer spring is fixedly connected to the lower end of the substrate. One end of the buffer spring away from the substrate is fixedly connected to the lower end of the placement groove.

[0009] Preferably, a hollow groove is provided in the middle of the protective bottle body.

[0010] Preferably, a protective cover is connected to the outside of the placement box through a hinge. A handle is fixedly connected to the outside of the protective cover.

[0011] Compared with the prior art, the beneficial effects of the present utility model are as follows: By providing a storage component, the protective bottle body rotates, and the lower end of the protective bottle body can be screwed into the inside of the lower cover plate, thereby forming a closed space to prevent external pollutants from contaminating the brain tissue, which is beneficial to the protection of the brain tissue. Moreover, the operation is simple, preventing damage to the brain tissue. And during the process of screwing the protective bottle body out or into the inside of the lower cover plate, there is no contact with the brain tissue, thus avoiding damage to the brain tissue caused by the protective bottle body, and providing more comprehensive protection for the brain tissue;

[0012] By providing a wedge block and a wedge groove, the wedge block can be inserted into the inside of the wedge groove, thereby fixing the lower cover plate, and further fixing the entire storage component, preventing the storage component from shaking when the experimenter moves the placement box, and ensuring the stability of the storage component;

[0013] By providing a vacuum groove, air and the protective bottle body will not become a conductor of temperature. When the brain tissue is placed in the lower cover plate, the temperature of the brain tissue can be kept constant for a long time and will not be quickly cooled or heated due to changes in the external temperature. This design ensures that the temperature of the brain tissue can be effectively maintained in various environments. Therefore, when the brain tissue is taken out of the medical refrigerator, it can still be in a low-temperature state for a long time. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0015] Figure 2 is a schematic diagram of the internal structure of the placement groove of the present utility model;

[0016] Figure 3 is an enlarged schematic diagram of the structure at A of the present utility model;

[0017] Figure 4Schematic structural diagram of the storage component of the present utility model.

[0018] The reference numerals in the figure are:

[0019] 1. Placing box; 101. Placing groove; 102. Protective cover; 103. Handle; 104. Substrate; 105. Buffer spring; 106. Wedge block; 107. Guide rod; 108. Limit spring;

[0020] 2. Storage component; 201. Lower cover plate; 202. Wedge groove; 203. Hollow groove; 204. Storage groove; 205. Protective bottle body. Specific embodiments

[0021] The following description is used to disclose the present utility model so that those skilled in the art can implement the present utility model. The preferred embodiments in the following description are only examples, and those skilled in the art can think of other obvious variations.

[0022] Embodiment 1

[0023] Please refer to Figures 1-4 As shown, a low-temperature storage device for brain tissue includes a placing box 1. At least six groups of placing grooves 101 are opened at the upper end of the placing box 1. At least six groups of storage components 2 are arranged inside the placing grooves 101. The storage component 2 includes a lower cover plate 201 and a protective bottle body 205. The lower cover plate 201 is movably connected inside the placing groove 101. The protective bottle body 205 is threadedly connected inside the lower cover plate 201. A storage groove 204 is arranged inside the lower cover plate 201. The protective bottle body 205 is movably connected to the outside of the storage groove 204. Two wedge grooves 202 are opened on the outside of the lower cover plate 201. Two wedge blocks 106 adapted to the wedge grooves 202 are slidably connected to the inner wall of the placing groove 101.

[0024] In this solution, multiple groups of storage components 2 can store brain tissues. They are centrally stored or classified and stored by placing the storage components 2 inside the placement box 1. The brain tissue can be placed inside the storage slot 204 in the middle of the lower cover plate 201. By rotating the protective bottle body 205 and fixing the lower cover plate 201 or making the lower cover plate 201 rotate in the opposite direction to the protective bottle body 205, the lower end of the protective bottle body 205 can be screwed into the inside of the lower cover plate 201, thus forming a closed space to prevent external pollutants from contaminating the brain tissue, which is beneficial to the protection of the brain tissue. The operation is simple, preventing damage to the brain tissue. If the brain tissue needs to be taken, the protective bottle body 205 is rotated in the reverse direction, so that the lower end of the protective bottle body 205 is screwed out of the lower cover plate 201. During the process of the protective bottle body 205 being screwed out or into the inside of the lower cover plate 201, there is no contact with the brain tissue, thus avoiding damage to the brain tissue caused by the protective bottle body 205 and providing more comprehensive protection for the brain tissue. Then, the lower cover plate 201 is placed inside the placement slot 101, and the wedge block 106 is slidably inserted into the wedge slot 202, so that the lower surface of the wedge block 106 abuts against the lower surface of the wedge slot 202, which can fix the lower cover plate 201, and further fix the entire storage component 2, preventing the storage component 2 from shaking when the experimenter moves the placement box 1 and ensuring the stability of the storage component 2.

[0025] Example 2

[0026] Please refer to Figure 3 As shown, a guide rod 107 is fixedly connected to the outside of the wedge block 106. The guide rod 107 is slidably connected inside the placement box 1. A limiting spring 108 is sleeved on the outside of the guide rod 107. The two ends of the limiting spring 108 are fixedly connected to the wedge block 106 and the placement box 1 respectively.

[0027] In this solution, the wedge block 106 can stably slide inside the placement box 1 through the guide rod 107, and the limiting spring 108 can push the wedge block 106 to be inserted into the wedge slot 202.

[0028] Example 3

[0029] Please refer to Figure 2 As shown, a base plate 104 is slidably connected inside the placement slot 101. A buffer spring 105 is fixedly connected to the lower end of the base plate 104. The end of the buffer spring 105 away from the base plate 104 is fixedly connected to the lower end of the placement slot 101.

[0030] In this solution, the substrate 104 can slide inside the placement groove 101. When the storage component 2 is placed inside the placement groove 101, the lower end of the lower cover plate 201 abuts against the upper surface of the substrate 104. The buffer spring 105 can buffer the storage component 2 through the substrate 104. If the placement box 1 drops to the ground or table or is collided, the impact force received by the storage component 2 can be reduced, thereby preventing damage to the brain tissue.

[0031] Furthermore, since the substrate 104 is in direct contact with the lower cover plate 201, the substrate 104 can be more optimally designed as a plate with a filling cavity. The substrate 104 is preferably provided with a liquid inlet and an outlet for introducing and discharging the quick-freezing liquid into and out of the filling cavity.

[0032] Furthermore, when it is necessary to take out the storage component 2, press down the protective bottle body 205 to drive the lower cover plate 201 to move downward, so that the upper side of the wedge-shaped groove 202 squeezes the inclined surface on the upper side of the wedge-shaped block 106, thereby driving the wedge-shaped block 106 to slide along the inclined surface of the wedge-shaped groove 202 into the interior of the placement box 1. At this time, rotate the protective bottle body 205 within the range of 15° - 165°, and drive the lower cover plate 201 to rotate, so that the wedge-shaped groove 202 and the wedge-shaped block 106 are not on the same vertical line. At this time, the storage component 2 can be taken out.

[0033] Example 4

[0034] Please refer to Figure 4 As shown, a hollow groove 203 is provided in the middle of the protective bottle body 205.

[0035] Rodent brain tissue has a relatively high water content, and 80% of the water will be frozen in the temperature range of -1 to -5 °C (this temperature range is called the maximum ice crystal formation zone), and ice crystals will form in the cell fluid. When the rodent heads stored in conventional tin foil are stored and sampled, they will experience multiple temperature rises and drops, and ice crystals are easily formed, damaging the observed structure. Larger ice crystals will cause greater damage to cells and tissues, affecting the clarity and integrity of tissue and cell morphology, and further affecting related molecular research such as immunohistochemistry, immunofluorescence, enzyme detection, tissue localization, and in situ hybridization.

[0036] In this solution, a hollow groove 203 is provided inside the protective bottle body 205. The hollow design is to achieve the heat preservation function, so that the air and the protective bottle body 205 will not become a conductor of temperature. When the brain tissue is placed in the lower cover plate 201, the temperature of the brain tissue can be kept constant for a long time and will not be quickly cooled or heated due to changes in the external temperature. This design ensures that the temperature of the brain tissue can be effectively maintained in various environments. Therefore, when the brain tissue is taken out of the medical refrigerator, it can still be in a low-temperature state for a long time.

[0037] Example 5

[0038] Please refer to Figure 1 As shown, a protective cover 102 is connected to the outside of the placement box 1 by a hinge, and a handle 103 is fixedly connected to the outside of the protective cover 102.

[0039] In this solution, the protective cover 102 can rotate and fit tightly with the upper end of the placement box 1, so that a closed space is formed between the placement box 1 and the protective cover 102, which is beneficial to the protection of the storage component 2 and further protects the brain tissue. The handle 103 facilitates the rotation of the protective cover 102.

[0040] The working principle and usage process of the present utility model are as follows: First, place the brain tissue inside the storage groove 204 in the middle of the lower cover plate 201. Then, invert the opening of the protective bottle body 205 inside the lower cover plate 201 and rotate the protective bottle body 205 to fix the lower cover plate 201 or make the lower cover plate 201 rotate in the opposite direction to the protective bottle body 205. Then, screw the lower end of the protective bottle body 205 into the inside of the lower cover plate 201 to form a closed space, completing the storage of the brain tissue. After that, place the storage component 2 storing the brain tissue inside the placement groove 101, and fix the lower cover plate 201 by sliding and inserting the wedge-shaped block 106 into the wedge-shaped groove 202, thereby fixing the entire storage component 2. When it is necessary to take out the storage component 2, press down on the protective bottle body 205 to drive the lower cover plate 201 and the substrate 104 to move downward, so that the wedge-shaped block 106 slides along the inclined surface of the wedge-shaped groove 202 into the inside of the placement box 1. At this time, rotate the protective bottle body 205 within the range of 15° - 165°, and drive the lower cover plate 201 to rotate, so that the wedge-shaped groove 202 and the wedge-shaped block 106 are not on the same vertical line. At this time, the storage component 2 can be taken out.

[0041] The above shows and describes the basic principles, main features and advantages of the present utility model. Those skilled in the art of this industry should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification is only the principle of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection claimed by the present utility model is defined by the appended claims and their equivalents.

Claims

1. A device for storing brain tissue at low temperature, comprising a placement box (1), characterized in that: At least six groups of placement grooves (101) are formed at the upper end of the placement box (1), and at least six groups of storage components (2) are arranged inside the placement grooves (101). The storage component (2) includes a lower cover plate (201) and a protective bottle body (205). The lower cover plate (201) is movably connected inside the placement groove (101), and the protective bottle body (205) is threadedly connected inside the lower cover plate (201). A storage groove (204) is arranged inside the lower cover plate (201), and the protective bottle body (205) is movably connected to the outside of the storage groove (204). Two wedge-shaped grooves (202) are formed on the outside of the lower cover plate (201), and two wedge-shaped blocks (106) adapted to the wedge-shaped grooves (202) are slidably connected to the inner wall of the placement groove (101).

2. The cryogenic storage device for brain tissue according to claim 1, wherein: A guide rod (107) is fixedly connected to the outside of the wedge-shaped block (106). The guide rod (107) is slidably connected inside the placement box (1). A limit spring (108) is sleeved on the outside of the guide rod (107). Two ends of the limit spring (108) are fixedly connected to the wedge-shaped block (106) and the placement box (1) respectively.

3. The cryogenic storage device for brain tissue according to claim 1, wherein: A substrate (104) is slidably connected inside the placement groove (101). A buffer spring (105) is fixedly connected to the lower end of the substrate (104). One end of the buffer spring (105) away from the substrate (104) is fixedly connected to the lower end of the placement groove (101).

4. The cryogenic storage device for brain tissue according to claim 1, characterized in that: A hollow groove (203) is arranged in the middle of the protective bottle body (205).

5. The cryogenic storage device for brain tissue according to claim 1, wherein: A protective cover (102) is connected to the outside of the placement box (1) through a hinge. A handle (103) is fixedly connected to the outside of the protective cover (102).

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