Tissue chip dot matrix puncher
By designing a tissue chip dot matrix hole puncher, using sampling tubes and push blocks set at multiple intervals, efficient and precise tissue chip hole punching is achieved, solving the problems of uneven arrangement and inaccurate depth control in traditional methods, and improving the reliability and efficiency of experimental results.
Patent Information
- Application Number
- CN202422077008.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-26
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2034-08-26
AI Technical Summary
The traditional tissue chip hole punching method has uneven arrangements, inaccurate depth control, complex operation and inefficient efficiency, which affects the quality of tissue chips and the repeatability of experimental results.
A tissue chip dot matrix hole puncher is designed, including a housing, a sampling tube, a push assembly and a compression spring. Through the cooperation of a multiple spaced sampling tube and a push block, an accurate and automated hole punching operation is achieved.
It improves the efficiency and accuracy of tissue chip preparation, reduces experimental errors, and is suitable for large-scale sample processing, especially in scenarios where large-scale tissue sample analysis is required in laboratories and hospitals.
Smart Images
Figure CN223084975U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of medical devices, and particularly to a tissue chip dot puncher. Background Art
[0002] During the preparation of tissue chips, punching is a crucial step, and its accuracy directly affects the accuracy and reliability of subsequent experiments. Traditional punching methods, such as manual punching or using non-specialized punching equipment, often have problems such as uneven alignment, inaccurate depth control, complex operation, and low efficiency. These problems may lead to uneven collection of tissue cores, thereby affecting the overall quality of tissue chips and the repeatability of experimental results. Therefore, developing a high-precision and easy-to-operate puncher is of great significance for improving the level of tissue chip preparation and promoting related scientific research. Content of the Utility Model
[0003] In view of this, this application proposes a tissue chip dot puncher to solve the above problems.
[0004] According to one aspect of this application, a tissue chip dot puncher is provided, including:
[0005] A housing, the interior of the housing is hollow, and a sampling tube extends from the front end face of the housing. The sampling tube is a tubular structure with a hollow interior. The number of sampling tubes is multiple, and the multiple sampling tubes are arranged at intervals.
[0006] A pushing assembly, the pushing assembly includes: a push block and a push rod;
[0007] The front end of the push rod is arranged inside the housing, and the rear end of the push rod is a free end and extends outward.
[0008] The push block is arranged at the front end of the push rod. An insertion rod is arranged on the front end face of the push block, and the insertion rod corresponds to the position of the sampling tube. The push block moves along the tube length direction of the sampling tube.
[0009] In a possible implementation, the sampling tubes are arranged in a rectangular array on the front end face of the housing, and the insertion rods are arranged in a rectangular array on the front end face of the push block.
[0010] In a possible implementation, the sampling tubes and the insertion rods are arranged in one-to-one correspondence, and the sizes of the sampling tubes and the insertion rods match each other.
[0011] In a possible implementation, it further includes: a compression spring, and the compression spring is sleeved on the push rod.
[0012] In one possible implementation, a limiting hole extends from the rear end face of the housing. The limiting hole is matched with the size of the push rod, and the push rod is inserted into the limiting hole.
[0013] In one possible implementation, the push rod and the push block are integrally formed, and a flange extends from the rear end of the push rod.
[0014] In one possible implementation, the diameter of the sampling tube ranges from 0.1 mm to 10 mm.
[0015] In one possible implementation, the sampling tube is a cylindrical tube and the insertion rod is a cylindrical rod.
[0016] In one possible implementation, the ratio of the rod length of the insertion rod to the tube length of the sampling tube is 1:1 - 3:2.
[0017] Advantages of the present application:
[0018] The tissue chip dot puncher of the present application has a simple structure and is easy to operate. It is used for the layout of the tissue chip template, with neat arrangement, uniform depth, and easy operation. Specifically, place the tissue chip dot puncher above the pre-prepared paraffin template to ensure that multiple sampling tubes can accurately align with the target area. Then adjust the position of the puncher so that the sampling tubes can punch holes at a predetermined interval and depth. By designing multiple sampling tubes arranged at intervals, the puncher can punch holes in the paraffin block, enabling multiple tissue chips to be placed in the processed paraffin block and then uniformly processing the tissue chips in the paraffin block, thereby greatly improving the efficiency of sample processing. Compared with the traditional single-hole punching method, this parallel processing method significantly shortens the experimental preparation time and is especially suitable for research or clinical applications that require large-scale sample processing. Secondly, the insertion rod provided at the front end of the push block corresponds to the position of the sampling tube, and the push block can accurately move along the tube length direction of the sampling tube, ensuring the accuracy and consistency of the punching position. This is particularly important for experiments that require strict control of the sample size and position, helping to reduce experimental errors and improve data reliability.
[0019] According to the following detailed description of the exemplary embodiments with reference to the accompanying drawings, other features and aspects of the present application will become clear. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] The drawings included in the specification and constituting a part of the specification, together with the specification, illustrate the exemplary embodiments, features, and aspects of the present application and are used to explain the principles of the present application.
[0021] Figure 1 A cross-sectional view of the tissue chip dot puncher showing an embodiment of the present application;
[0022] Figure 2 Schematic diagram of a paraffin template processed by using the tissue chip dot puncher according to an embodiment of the present application. Detailed implementation manners
[0023] Various exemplary embodiments, features and aspects of the present application will be described in detail below with reference to the accompanying drawings. The same reference numerals in the drawings denote elements having the same or similar functions. Although various aspects of the embodiments are shown in the drawings, the drawings are not necessarily drawn to scale unless otherwise specified.
[0024] Among them, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application or simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present application.
[0025] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, "a plurality" means two or more unless otherwise specifically defined.
[0026] The special term "exemplary" here means "serving as an example, an embodiment or an illustration". Any embodiment described as "exemplary" here does not have to be construed as superior to or better than other embodiments.
[0027] In addition, in order to better illustrate the present application, numerous specific details are given in the following detailed implementation manners. Those skilled in the art should understand that the present application can also be implemented without some specific details. In some instances, methods, means, elements and circuits well-known to those skilled in the art are not described in detail so as to highlight the gist of the present application.
[0028] Such as Figure 1 And 2As shown in the figure, the tissue chip dot matrix puncher includes: a housing 100, the interior of the housing 100 is hollow, and a sampling tube 110 extends from the front end face of the housing 100. The sampling tube 110 is a tubular structure with a hollow interior. The number of sampling tubes 110 is multiple, and the multiple sampling tubes 110 are arranged at intervals. A pushing assembly 200, the pushing assembly 200 includes: a push block 220 and a push rod 210; the front end of the push rod 210 is arranged inside the housing 100, and the rear end of the push rod 210 is a free end and extends outward; the push block 220 is arranged at the front end of the push rod 210, and a plug rod 221 is arranged on the front end face of the push block 220, and the plug rod 221 corresponds to the position of the sampling tube 110, and the push block 220 moves along the tube length direction of the sampling tube 110.
[0029] The tissue chip dot matrix puncher in this embodiment has a simple structure and is used for the layout of the tissue chip template, with neat arrangement, uniform depth and simple operation. Specifically, place the tissue chip dot matrix puncher above the pre-prepared paraffin template to ensure that the multiple sampling tubes 110 can accurately align with the target area. Then adjust the position of the puncher so that the sampling tubes 110 can sample at a predetermined interval and depth. Hold the outer housing 100 and press down the sampling tube 110. At this time, the cut paraffin block is located in the hole of the sampling tube 110. Pull out the sampling tube 110, and then use the rear end of the push rod 210 to press down the push rod 210, so that the push block 220 and the plug rod 221 push down the paraffin block in the hole of the sampling tube 110, causing the paraffin block to exit the hole of the sampling tube 110. Release the pressed plug rod 221, and the compression spring 300 retracts the flange 211, the push block 220 and the plug rod 221 assembly to the starting position. Discard the paraffin block in the hole of the sampling tube 110 to obtain the substrate tissue chip template. By designing multiple sampling tubes 110 arranged at intervals, the puncher can realize the punching operation of the tissue chip template at one time, greatly improving the efficiency of tissue chip preparation. Compared with the traditional single-hole punching method, this parallel processing method significantly shortens the experimental preparation time, especially suitable for research or clinical applications that require large-scale sample processing. Among them, the plug rod 221 arranged at the front end of the push block 220 corresponds to the position of the sampling tube 110, ensuring that the reserved positions in the tissue chip template are neatly distributed after punching. This is particularly important for experiments that require strict control of sample size and position, helping to reduce experimental errors and improve data reliability. Secondly, the hollow design inside the housing 100 and the compact layout of the sampling tube 110 and the pushing assembly 200 make the whole puncher structure compact and light, convenient for carrying and storage.
[0030] In one specific embodiment, the sampling tubes 110 are arranged in a rectangular array on the front end face of the housing 100, and the insertion rods 221 are arranged in a rectangular array on the front end face of the push block 220. In this embodiment, it should be noted that the sampling tubes 110 are arranged in a rectangular array on the front end face of the housing 100, enabling the puncher to process multiple sample points simultaneously, greatly enhancing the ability of batch sample collection and processing, and being particularly suitable for scenarios such as laboratories and hospitals that require a large amount of tissue sample analysis. Secondly, it should be noted that through a centralized and automated punching method, the risk of cross-contamination during the operation process is reduced. Especially when dealing with biological samples, this is particularly important, which helps to ensure the accuracy and reliability of the experimental results. In addition, key components such as the sampling tubes 110 and the insertion rods 221 are made of wear-resistant and corrosion-resistant materials, ensuring the long service life and reliability of the puncher.
[0031] In one specific embodiment, the sampling tubes 110 and the insertion rods 221 are arranged in one-to-one correspondence, and the sizes of the sampling tubes 110 and the insertion rods 221 match. In this embodiment, it should be noted that since the number and arrangement of the sampling tubes 110 and the insertion rods 221 can be adjusted according to actual needs, the puncher can flexibly adapt to different sizes and shapes of tissue samples, as well as different punching requirements, enhancing its versatility and practicality. It should also be noted that the rear end of the insertion rod 221 is arranged in the middle of the push block 220, and the front end of the insertion rod 221 is inserted into the middle and rear part of the sampling tube 110.
[0032] In one specific embodiment, it further includes: a compression spring 300, and the compression spring 300 is sleeved on the push rod 210. In this embodiment, it should be noted that the compression spring 300 provides the force for the automatic reset of the push rod 210. When the user applies a thrust to the push rod 210 to push the push block 220 and the insertion rod 221 to complete the punching operation, and releases the push rod 210, the compression spring 300 will automatically pull the push rod 210 back to the initial position using its elastic potential energy, without the need for the user to manually reset, improving the convenience and continuity of the operation. Secondly, during the punching process by those skilled in the art, the compression spring 300 can absorb part of the impact force, playing a buffering and protective role for components such as the push rod 210, the push block 220, and the insertion rod 221, reducing the wear and damage caused by direct impact, and extending the service life of the device. Further, it should be noted that by adjusting the pre-tightening force and length of the compression spring 300, the forward movement distance of the push block 220 and the insertion rod 221, that is, the punching depth, can be controlled to a certain extent. This design enables the user to adjust the punching depth according to needs, meeting the accuracy requirements of different experiments or diagnoses. And the presence of the compression spring 300 makes the push rod 210 move more smoothly during the movement, reducing the problem of inaccurate punching positions caused by vibration or deviation. This helps to improve the punching accuracy and consistency, and ensure the quality of sample collection.
[0033] In one specific embodiment, the rear end face of the housing 100 extends with a limiting hole, the limiting hole matches the size of the push rod 210, and the push rod 210 is inserted into the limiting hole. In this embodiment, it should be noted that the limiting hole provides a stable and precise guide channel for the push rod 210. When the push assembly 200 is working, the push rod 210 can move smoothly and linearly in the limiting hole, avoiding the push rod 210 from deflecting or shaking during the movement, thereby ensuring that the push block 220 and the insertion rod 221 thereon can be accurately aligned and inserted into the sampling tube 110 to achieve precise punching operation. The limiting hole limits the moving range of the push rod 210, thereby also limiting the moving distance of the push block 220. This design helps to accurately control the punching depth and prevent the insertion rod 221 from being over-inserted into the sample to cause damage or destruction. At the same time, it also ensures the consistency of each punching operation, improving work efficiency and the reliability of results. Secondly, by setting a limiting hole on the rear end face of the housing 100 and closely matching with the push rod 210, this design enhances the stability and durability of the entire punch structure. The limiting hole serves as a moving fulcrum for the push rod 210, shares part of the thrust, reduces the stress burden on other parts of the housing 100, and helps prevent the housing 100 from being deformed or damaged due to long-term stress.
[0034] In one specific embodiment, the push rod 210 and the push block 220 are integrally formed, and a flange 211 extends from the rear end of the push rod 210. In this embodiment, it should be noted that the integrally formed design allows the push rod 210 and the push block 220 to be seamlessly connected, avoiding gaps or weaknesses that may exist in traditional connection methods, thereby enhancing the structural strength of the entire push assembly 200 and extending the service life of the device. Secondly, the integrally formed push assembly 200 can more accurately control the size and shape during the manufacturing process, ensuring the alignment accuracy between the insertion rod 221 on the push block 220 and the sampling tube 110, thereby improving the accuracy and consistency of the punching. The flange 211 provides an area for the implementers in this field to hold and operate conveniently. The user can apply a thrust by holding the flange 211 to move the push rod 210 and the push block 220 forward, thereby completing the punching operation. In some cases, the flange 211 can also serve as a limiter to prevent the push rod 210 from being pushed in too far. When the push rod 210 moves to a certain position, the flange 211 contacts a certain part of the housing 100 and generates resistance, thereby preventing the push rod 210 from continuing to move forward. This design helps prevent the insertion rod 221 from being over-inserted into the sample and causing damage.
[0035] In one specific embodiment, the diameter of the sampling tube 110 ranges from 0.1 mm to 10 mm. In this embodiment, it should be noted that the common preferred diameters of the sampling tube 110 are 1 mm, 1.5 mm, 2 mm, and 3 mm. The specific diameter of the sampling tube 110 can be selected according to the actual situation as long as the sampling requirements are met. In addition, it should also be noted that the sampling tube 110 extends 1.5 cm - 2 cm from the front end face of the housing 100, and the preferred length of the sampling tube 110 is 4 cm - 4.5 cm.
[0036] As Figure 2 shown, after the paraffin block 400 is punched by a tissue microarray spotting punch, a paraffin block 400 with an array of round holes 410 as Figure 2 shown is formed. The grid of the round holes 410 of the paraffin template 400 matches the size of the sampling tube 110. After the corresponding paraffin block is taken out by the sampling tube, the originally smooth paraffin block is prepared into Figure 2 a paraffin template 400, and the grid of the round holes 410 of the paraffin template 400 therein is suitable for placing tissue microarrays. In this embodiment, it should be noted that the paraffin block 400 is commonly selected to be prepared into a rectangular array structure containing round holes 410 such as 8*8, 7*9, 6*6, 4*7, 5*5, etc., and the paraffin block 400 needs to have a 2 mm margin, with a hole diameter of 3 mm, which is suitable for sampling tubes 110 smaller than 3 mm, such as 2 mm and 2.5 mm.
[0037] In one specific embodiment, the sampling tube 110 is a cylindrical tube and the insertion rod 221 is a cylindrical rod. It should be noted that the sampling tube 110 is a cylindrical tube with a hollow interior, and the inner diameter of the sampling tube 110 is adapted to the outer diameter of the insertion rod 221.
[0038] In one specific embodiment, the ratio of the rod length of the insertion rod 221 to the tube length of the sampling tube 110 is: 1:1 - 3:2.
[0039] It should be noted that although the tissue microarray spotting punch has been introduced by taking this application as an example above, those skilled in the art can understand that this application should not be limited thereto. In fact, users can flexibly set parameters according to personal preferences and / or actual application scenarios as long as it is reasonable.
[0040] The above has described the embodiments of this application. The above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and variations are obvious to those of ordinary skill in the art in the technical field without departing from the scope and spirit of the described embodiments. The choice of terms used herein is intended to best explain the principles of the embodiments, actual applications, or improvements to the technologies in the market, or to enable other ordinary skilled persons in the technical field to understand the disclosed embodiments herein.
Claims
1. A tissue microarray dot puncher, characterized in that, Comprising: A housing, the interior of the housing is hollow, and a sampling tube extends from the front end face of the housing. The sampling tube is a tubular structure with a hollow interior. The number of sampling tubes is multiple, and the multiple sampling tubes are arranged at intervals. A pushing assembly, the pushing assembly includes: a pushing block and a push rod; The front end of the push rod is arranged inside the housing, and the rear end of the push rod is a free end and extends outwards; The pushing block is arranged at the front end of the push rod. A plug rod is arranged on the front end face of the pushing block, and the plug rod corresponds to the position of the sampling tube. The pushing block moves along the tube length direction of the sampling tube.
2. The tissue microarray dot puncher according to claim 1, wherein, The sampling tubes are arranged in a rectangular array on the front end face of the housing, and the plug rods are arranged in a rectangular array on the front end face of the pushing block.
3. The tissue microarray dot puncher according to claim 1, wherein The sampling tubes and the plug rods are arranged in one-to-one correspondence, and the sampling tubes and the plug rods are matched in size.
4. The tissue chip dot matrix puncher according to claim 1, wherein, Further comprising: A compression spring, the compression spring is sleeved on the push rod.
5. The tissue microarray dot puncher according to claim 1, wherein A limiting hole extends from the rear end face of the housing, and the limiting hole is matched in size with the push rod. The push rod is inserted into the limiting hole.
6. The tissue microarray dot puncher according to claim 1, wherein The push rod and the pushing block are integrally formed, and a flange extends from the rear end of the push rod.
7. The tissue chip dot matrix puncher according to claim 1, characterized in that, The diameter of the sampling tube ranges from 0.1 mm to 10 mm.
8. The tissue chip dot matrix puncher according to any one of claims 1-7, characterized in that, The sampling tube is a cylindrical tube, and the plug rod is a cylindrical rod.
9. The tissue chip dot matrix punching device according to claim 8, characterized in that, The ratio of the rod length of the plug rod to the tube length of the sampling tube is: 1:1 - 3:2.