Biological reaction container cover plate and polymerase chain reaction container
By designing a bioreaction container cover with elastic support pad and sealing layer, the problem of insufficient reliability of the sealing cover film in PCR experiments is solved, sealing and convenience are achieved, and the accuracy of experimental results and sample integrity are ensured.
Patent Information
- Application Number
- CN202422161959.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-04
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2034-09-04
AI Technical Summary
In the existing PCR experiments, the reliability of the sealing cover film is insufficient, resulting in the invasion of external pollutants and the leakage of internal reaction substances. The sealing cover film is difficult to separate easily, affecting the accuracy of the experimental results and sample integrity.
A bioreaction container cover is designed, using a combination of an elastic support pad and a sealing layer. The support pad is compressed and sealed under external force, and restores to its original state after the external force is removed, achieving reliable separation between the cover plate and the container, ensuring sealing and convenient opening.
It improves the sealing and convenience of the experiment, avoids sample spilling and cross-contamination, ensures the accuracy of experimental results and sample integrity, and reduces maintenance costs.
Smart Images
Figure CN223087808U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of biological reaction containers, and in particular relates to a biological reaction container cover plate and a polymerase chain reaction container. Background Art
[0002] In biomedical research, with the advancement of science and technology and the growth of experimental needs, the demand for batch experiments is increasing, and multi-hole reaction vessels have been widely used. In genomics research, researchers often need to process hundreds or thousands of samples for high-throughput screening, gene sequencing, and other tasks.
[0003] Take polymerase chain reaction (PCR) as an example. As an extremely important molecular biology technology, it is widely used in many fields such as gene diagnosis, genetic disease detection, and forensic identification. PCR technology requires multiple rounds of amplification under precisely controlled temperature conditions to achieve the purpose of rapid amplification of specific DNA sequences. In order to ensure the accuracy and reliability of the PCR process, the sealing of the reaction system must be strictly controlled to prevent the entry of foreign contaminants and cross-contamination of the reaction mixture.
[0004] At present, the reaction vessels used in PCR experiments are mostly compressed and sealed with disposable flat sealing covers. The reliability of this sealing method is relatively weak. Once external impurities invade or internal reaction substances leak during the experiment, the accuracy of the experimental results will be affected. On the other hand, it is very inconvenient to remove the sealing cover film after the experiment is completed. Since the sealing cover film is always pressed on the surface of the reaction vessel by external force during the experiment, a certain adsorption effect is formed between the sealing cover film and the reaction vessel. When the operator removes the sealing cover film, the reaction vessel is lifted or moved together, resulting in the violent shaking of the materials in the reaction vessel and spillage or cross-contamination, which destroys the integrity of the experimental samples, resulting in the inability to further verify the test results of the samples, and the inability to further analyze and study the samples after the test. Utility Model Content
[0005] In view of the deficiencies in the prior art, the utility model provides a bioreactor container cover and a polymerase chain reaction container, the purpose of which is to achieve effective sealing of the opening of the bioreactor container while ensuring that the cover can be easily separated after use to avoid material spillage or cross contamination, and a further purpose is to improve the usability, durability and convenience of maintenance of the cover.
[0006] The first aspect of the present utility model is to provide a cover plate for a bioreactor, which includes a cover plate body in the shape of a flat plate and a surrounding frame extending inward from the edge of the cover plate body; a sealing layer is provided on the inner side of the cover plate body, and the sealing layer has a flat inner surface for covering the upper surface of the bioreactor to seal the opening on the upper surface of the bioreactor; a plurality of support pads are provided in the spaced area between the surrounding frame and the sealing layer on the inner side of the cover plate body, the support pads are elastic and support on the upper surface of the bioreactor; when the support pads are externally squeezed, the height decreases, so that the inner surface of the sealing layer contacts the upper surface of the bioreactor; when the squeezing force on the support pads is withdrawn, the height increases, so that the inner surface of the sealing layer is separated from the upper surface of the bioreactor.
[0007] As a further optimized scheme of the above-mentioned cover plate for a bioreactor, the cover plate body is rectangular, a rectangular spaced area is formed between the surrounding frame and the sealing layer, and 3 to 10 support pads are linearly arranged on any one of the four sides of the spaced area.
[0008] As a further optimized scheme of the above-mentioned cover plate for a bioreactor, the support pads are columnar, and a plurality of receiving holes are provided in the spaced area between the surrounding frame and the sealing layer on the inner side of the cover plate body, and one end of the support pad is assembled in the receiving hole.
[0009] As a further optimized scheme of the above-mentioned cover plate for a bioreactor, a support boss is formed on the outer side of the cover plate body corresponding to each receiving hole; when a plurality of cover plates for a bioreactor are stacked up and down, the support boss on the lower cover plate for a bioreactor supports the support pad on the upper cover plate for a bioreactor, separating the upper surface of the lower cover plate for a bioreactor from the sealing layer of the upper cover plate for a bioreactor.
[0010] As a further optimized scheme of the above-mentioned cover plate for a bioreactor, the support pads are rubber pads or silica gel pads.
[0011] As a further optimized scheme of the above-mentioned cover plate for a bioreactor, a limiting retaining ring is provided on the outer side of the cover plate body along the edge of the cover plate body; when a plurality of cover plates for a bioreactor are stacked up and down, the limiting retaining ring on the lower cover plate for a bioreactor is restricted within the surrounding frame of the upper cover plate for a bioreactor.
[0012] As a further optimized scheme of the above-mentioned cover plate for a bioreactor, anti-slip areas are also relatively provided on the outer side wall of the surrounding frame, and horizontal anti-slip lines are provided in the anti-slip areas.
[0013] As a further optimized scheme of the above-mentioned cover plate for a bioreactor, a plurality of vertically extending partition strips are also provided on the inner side wall of the surrounding frame.
[0014] As a further optimized solution for the cover plate of the above biological reaction vessel, the sealing layer is a silicone film layer pasted or formed on the inner side of the cover plate body.
[0015] The second aspect of the present utility model is to provide a polymerase chain reaction vessel, which includes a vessel body for accommodating polymerase chain reaction substances and a cover plate that can be covered above the vessel body; the vessel body has an upper surface, and a plurality of reaction cavities are formed on the upper surface in an array distribution and are independent of each other; the cover plate uses the cover plate of the above biological reaction vessel; when the support pad is extruded by an external force, its height decreases, allowing the inner surface of the sealing layer to squeeze the upper surface of the vessel body and seal the reaction cavities; when the extrusion force on the support pad is withdrawn, its height increases, causing the inner surface of the sealing layer to separate from the upper surface of the vessel body.
[0016] Beneficial effects
[0017] Compared with the prior art, the cover plate of the biological reaction vessel provided by the present utility model can be repeatedly used to seal the reaction vessel, which can effectively improve the convenience, effectiveness and repeatability of biological experiments and ensure the integrity of samples after experiments. When the cover plate is pressed down by an external force, the support pad can be compressed, prompting the sealing layer to closely adhere to the opening of the reaction vessel, thereby achieving a good sealing effect. When the external force is withdrawn, the support pad returns to its original state by virtue of its elasticity, prompting the sealing layer to separate from the surface of the vessel, avoiding mutual adsorption between the cover plate and the vessel, and preventing the operator from lifting the vessel together when removing the cover plate, ensuring the integrity of the experimental samples and the feasibility of subsequent analysis.
[0018] The cover plate of the biological reaction vessel provided by the present utility model can be applied to various types of biological reaction vessels. Especially in sensitive experiments such as polymerase chain reaction, it can effectively ensure the integrity of samples and the accuracy of experimental results.
[0019] In addition, the cover plate of the biological reaction vessel provided by the present utility model also has the advantages that the support pad can be conveniently installed and replaced, the material is stable and durable, it has anti-slip performance and can be stacked, significantly improving the efficiency and comfort of experimental operations, while reducing the maintenance cost, and providing a reliable solution for experiments in the fields of biology, medicine, etc. Description of the drawings
[0020] Figure 1 and Figure 2 is a three-dimensional schematic diagram of the cover plate of the biological reaction vessel.
[0021] Figure 3 is a top view of the cover plate of the biological reaction vessel.
[0022] Figure 4 is Figure 3 a cross-sectional view taken along the C-C plane in
[0023] Figure 5 isFigure 3 Cross-sectional view of plane E-E.
[0024] Figure 6 It is a schematic structural diagram of a polymerase chain reaction container.
[0025] In the figure: 1. Cover plate body; 2. Surrounding frame; 3. Sealing layer; 4. Support pad; 5. Support boss; 6. Limit retaining ring; 7. Anti-slip area; 8. Partition strip; 9. Container body. Specific implementation mode
[0026] The following further clarifies the present utility model through embodiments, aiming to more clearly illustrate the technical solutions of the present utility model and should not be construed as a limitation.
[0027] Embodiment 1
[0028] As Figures 1 to 3 shown, a cover plate of a biological reaction container includes a cover plate body 1 in the shape of a flat plate and a surrounding frame 2 extending inward from the edge of the cover plate body 1; a sealing layer 3 is provided on the inner side of the cover plate body 1, and the sealing layer 3 has a flat inner surface for covering the upper surface of the biological reaction container to seal the opening on the upper surface of the biological reaction container; a plurality of support pads 4 are provided in the interval area between the surrounding frame 2 and the sealing layer 3 on the inner side of the cover plate body 1, the support pads 4 are elastic and support on the upper surface of the biological reaction container; when the support pads 4 are externally pressed, the height decreases, so that the inner surface of the sealing layer 3 contacts the upper surface of the biological reaction container; when the external pressure applied to the support pads 4 is withdrawn, the height increases, so that the inner surface of the sealing layer 3 is separated from the upper surface of the biological reaction container.
[0029] The cover plate of the biological reaction container can be applied to experiments in the fields of biology, medicine, etc. and is used as a cover plate to cover the opening above the reaction container. The inner side of the cover plate body 1 has a flat sealing layer 3, and the interval area between the surrounding frame 2 and the sealing layer 3 has elastic support pads 4. In this way, when the cover plate of the biological reaction container is externally pressed downward, the sealing layer 3 on the inner side of the cover plate can closely fit the opening of the reaction container, thereby achieving effective sealing. When the external force is removed, the elastic support pads 4 tend to return to their original height and apply an upward force to the cover plate until the sealing layer 3 is separated from the upper surface of the reaction container, avoiding the cover plate being adsorbed to the reaction container.
[0030] In some biological reaction experiments, the downward pressure that makes the sealing layer 3 closely fit the reaction vessel can come from automated experimental instruments. For example, when performing polymerase chain reaction, the reaction vessel covered with the cover plate is placed in the test instrument, and the instrument will automatically apply a certain pressure to the cover plate. At this time, the height of the support pad 4 decreases after being pressed, prompting the sealing layer 3 to closely adhere to and seal the opening part of the biological reaction vessel, ensuring that there is no leakage or contamination during the reaction process. When the test is over, the support pad 4 returns to its original shape due to its elasticity, causing the inner surface of the sealing layer 3 to separate from the upper surface of the container, facilitating the user to easily open the cover plate.
[0031] Experiments have found that when the cover plate tightly seals the opening of the reaction vessel, a strong adsorption force is formed between the cover plate and the container mouth. When the operator lifts the cover plate, it is very easy to drive the reaction vessel to be lifted together, increasing the risk of vibration and dropping of the reaction vessel. Once the reaction vessel is lifted and dropped, it is easy for the samples in the container to spill out. In addition, there are often multiple different samples in the reaction vessel, and it is easy for multiple samples to cross-contaminate when dropped. Whether the sample spills or cross-contaminates, it will cause irreversible damage to the sample, resulting in the inability to check the test results of the sample subsequently and the inability to further analyze and study the sample. Using the above-mentioned cover plate for biological reaction vessels can effectively avoid this problem, ensure the integrity of the sample after testing, and at the same time help improve the reliability and repeatability of the experimental results.
[0032] As Figure 2 shown, the cover plate body 1 is rectangular, and a rectangular spacer area is formed between the surrounding frame 2 and the sealing layer 3. On any one of the four sides of the spacer area, 3 to 10 support pads 4 are linearly arranged. Such a layout can make the distribution of the support pads 4 more uniform on the entire cover plate, so as to ensure that the cover plate can fit more smoothly when contacting the reaction vessel, avoiding the occurrence of poor sealing caused by excessive local stress. When the test is completed, the process of the support pad 4 returning to its original shape causes the cover plate to be evenly lifted by the support pads 4 around, ensuring that the sealing layer 3 is completely separated from the container surface.
[0033] As Figure 2 shown, the support pad 4 is preferably columnar, and a plurality of receiving holes are provided inside the cover plate body 1 and in the spacer area between the surrounding frame 2 and the sealing layer 3. One end of the support pad 4 is assembled in the receiving hole, which can facilitate the installation and replacement of the support pad 4. When a certain support pad 4 is damaged, it can be replaced separately, thus reducing the maintenance cost.
[0034] As Figure 3 and Figure 5As shown in the figure, support bosses 5 are formed on the outer side of the cover plate body 1 corresponding to each accommodating hole; when a plurality of biological reaction vessel cover plates are stacked vertically, the support bosses 5 on the lower biological reaction vessel cover plate support the support pads 4 on the upper biological reaction vessel cover plate, separating the upper surface of the lower biological reaction vessel cover plate from the sealing layer 3 of the upper biological reaction vessel cover plate, which not only facilitates the vertical stacking of a plurality of cover plates, but also avoids cross-contamination between the upper and lower cover plates.
[0035] Preferably, the support pad 4 is a rubber pad or a silica gel pad. Rubber pads and silica gel pads not only do not produce sharp noises during use, but also have excellent elastic recovery ability, good chemical resistance, temperature stability, safety, and easy processability, ensuring that the support pad 4 can reliably exert its elastic force under various experimental conditions.
[0036] As Figure 3 and Figure 5 shown in the figure, a limit retaining ring 6 is provided on the outer side of the cover plate body 1 along the edge of the cover plate body 1; when a plurality of biological reaction vessel cover plates are stacked vertically, the limit retaining ring 6 on the lower biological reaction vessel cover plate is restricted within the surrounding frame 2 of the upper biological reaction vessel cover plate, preventing the biological reaction vessel cover plates from slipping during stacking and causing contamination.
[0037] As Figure 1 and Figure 2 shown in the figure, anti-slip areas 7 are also provided oppositely on the outer side wall of the surrounding frame 2, and transverse anti-slip patterns are provided in the anti-slip areas 7. The anti-slip patterns in the anti-slip areas 7 not only help the operator to stably lift the cover plate upward, but also indicate a reasonable grasping position for the operator visually and by touch.
[0038] As Figure 2 and Figure 4 shown in the figure, a plurality of vertically extending partition strips 8 are also provided on the inner side wall of the surrounding frame 2.
[0039] Preferably, the sealing layer 3 is a silica gel film layer pasted or formed on the inner side of the cover plate body 1. Silica gel has good chemical stability and can resist the erosion of a variety of chemicals, effectively coping with biological reactions with different environmental requirements. The silica gel material also has good resilience, which enables the sealing layer 3 to closely fit the container opening when pressed and quickly return to its original state after the pressure is released, ensuring the sealing effect.
[0040] Embodiment 2
[0041] As Figure 6A polymerase chain reaction container as shown includes a container body 9 for accommodating polymerase chain reaction reactants and a cover plate that can be covered above the container body 9; the container body 9 has an upper surface, and a plurality of reaction cavities that are arrayed and independent of each other are formed on the upper surface; the cover plate is the cover plate of the biological reaction container provided in Embodiment 1; when the support pad 4 is extruded by an external force, its height decreases, allowing the inner surface of the sealing layer 3 to extrude the upper surface of the container body 9 and seal the reaction cavities; when the extrusion force applied to the support pad 4 is withdrawn, its height increases, causing the inner surface of the sealing layer 3 to separate from the upper surface of the container body 9, preventing the operator from lifting the lower container body 9 together when lifting the cover plate after the polymerase chain reaction.
[0042] The above embodiments are exemplary. The purpose is to illustrate the technical concept and characteristics of the present invention so that those skilled in this field can understand the content of the present invention and implement it accordingly, and it should not be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit and essence of the present invention should be covered within the protection scope of the present invention.
Claims
1. A cover plate for a biological reaction vessel, characterized in that: It includes a cover plate body (1) in the shape of a flat plate and a surrounding frame (2) extending inward from the edge of the cover plate body (1); a sealing layer (3) is provided inside the cover plate body (1), and the sealing layer (3) has a flat inner surface for covering the upper surface of the bioreactor to seal the opening on the upper surface of the bioreactor; a number of support pads (4) are provided in the spaced area inside the cover plate body (1) and between the surrounding frame (2) and the sealing layer (3), the support pads (4) are elastic and support on the upper surface of the bioreactor; when the support pads (4) are externally pressed, the height decreases, so that the inner surface of the sealing layer (3) contacts the upper surface of the bioreactor; when the pressing force on the support pads (4) is withdrawn, the height increases, so that the inner surface of the sealing layer (3) separates from the upper surface of the bioreactor.
2. The biological reaction vessel cover plate according to claim 1, wherein: The cover plate body (1) is rectangular, a rectangular spaced area is formed between the surrounding frame (2) and the sealing layer (3), and on any one of the four sides of the spaced area, 3 to 10 of the support pads (4) are linearly arranged.
3. The biological reaction vessel cover plate according to claim 2, wherein: The support pads (4) are columnar, and a plurality of receiving holes are provided in the spaced area inside the cover plate body (1) and between the surrounding frame (2) and the sealing layer (3), and one end of the support pad (4) is assembled in the receiving hole.
4. The biological reaction vessel cover plate according to claim 3, characterized in that: Support bosses (5) are formed outside the cover plate body (1) and corresponding to each receiving hole; when a plurality of bioreactor covers are stacked one on top of the other, the support bosses (5) on the lower bioreactor cover support the support pads (4) on the upper bioreactor cover, separating the upper surface of the lower bioreactor cover from the sealing layer (3) of the upper bioreactor cover.
5. The biological reaction vessel cover plate according to any one of claims 1 to 4, characterized in that: The support pads (4) are rubber pads or silicone pads.
6. The biological reaction vessel cover plate according to claim 5, wherein: A limiting retaining ring (6) is provided outside the cover plate body (1) and along the edge of the cover plate body (1); when a plurality of bioreactor covers are stacked one on top of the other, the limiting retaining ring (6) on the lower bioreactor cover is restricted within the surrounding frame (2) of the upper bioreactor cover.
7. The biological reaction vessel cover plate according to claim 5, characterized in that: Anti-slip areas (7) are also provided oppositely on the outer sidewall of the surrounding frame (2), and horizontal anti-slip patterns are provided in the anti-slip areas (7).
8. The biological reaction vessel cover plate according to claim 5, characterized in that: A number of vertically extending partition strips (8) are also provided on the inner sidewall of the surrounding frame (2).
9. The biological reaction vessel cover plate according to claim 5, wherein: The sealing layer (3) is a silicone film layer pasted or formed on the inside of the cover plate body (1).
10. A polymerase chain reaction container, characterized in that: It includes a container body (9) for accommodating polymerase chain reaction reagents and a cover plate that can be covered above the container body (9); the container body (9) has an upper surface, and a plurality of reaction cavities are formed on the upper surface in an array distribution and are independent of each other; the cover plate is the cover plate of the bioreaction container according to any one of claims 1 to 9; when the support pad (4) is extruded by an external force, its height decreases, allowing the inner surface of the sealing layer (3) to extrude the upper surface of the container body (9) and seal the reaction cavities; when the extrusion force applied to the support pad (4) is withdrawn, its height increases, causing the inner surface of the sealing layer (3) to separate from the upper surface of the container body (9).