Automatic ergothioneine extraction and purification equipment

Abstract

The utility model discloses automatic ergothioneine extraction and purification equipment which comprises an ergothioneine extraction and purification equipment body and a placement heating box arranged at the upper end of the ergothioneine extraction and purification equipment body, an outer connecting ring body is arranged at the outer side position of the discharging port, a built-in connecting spring body is arranged at the tail end position of the slope lifting block, the outer connecting ring body is rotationally connected with the discharging port through threads, and during use, when the outer connecting ring body is extruded by gas, the slope position of the slope lifting block can be extruded so that the slope lifting block can move towards the side face; after the extrusion force disappears, the slope lifting block can be reset again for sealing due to the elasticity of the built-in connecting spring body, and the discharge port can be effectively sealed in a self-sealing manner after the product stops flowing out; therefore, the phenomenon that the quality of subsequent products is influenced by the pollution of the discharge port caused by the external environment can be effectively avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field related to biotechnology, and particularly relates to an automated ergothioneine extraction and purification device. Background Art

[0002] Ergothioneine is a sulfur-containing amino acid with antioxidant effects. It is a natural cell protectant and antioxidant in the human body. It can not only scavenge free radicals in cells but also reverse oxidized VC in cells. It is a very popular anti-aging star ingredient nowadays.

[0003] A large number of studies on ergothioneine at home and abroad have confirmed that it has significant effects in aspects such as sunscreen, whitening, anti-aging, radiation protection, inhibition of inflammation, and reduction of glycosylation.

[0004] An automated ergothioneine extraction and purification device is a device used for extracting and purifying ergothioneine.

[0005] However, when the automated ergothioneine extraction and purification device discharges products, it is very easy for the products to be contaminated by the outside due to residues at the discharge pipe orifice, which in turn affects the quality of the subsequent flowing-out products.

[0006] The utility model improves the above problems and particularly relates to an automated ergothioneine extraction and purification device capable of self-sealing. Summary of the Utility Model

[0007] The purpose of the utility model is to provide an automated ergothioneine extraction and purification device to solve the problem that when the automated ergothioneine extraction and purification device discharges products, it is very easy for the products to be contaminated by the outside due to residues at the discharge pipe orifice, which in turn affects the quality of the subsequent flowing-out products as mentioned in the above background art.

[0008] To achieve the above purpose, the utility model provides the following technical solution: an automated ergothioneine extraction and purification device, including an ergothioneine extraction and purification device body and a placement heating box arranged at the upper end position of the ergothioneine extraction and purification device body;

[0009] A discharge port is arranged at the right side position of the ergothioneine extraction and purification device body;

[0010] An outer connection ring body is arranged at the outer side position of the discharge port. A slope lifting block is arranged inside the outer connection ring body. An internal connection spring body is arranged at the end of the slope lifting block. The outer connection ring body is rotationally connected to the discharge port through threads. During use, when the outer connection ring body is squeezed by gas, the slope position of its slope lifting block will be squeezed, causing it to move to the side. When the squeezing force disappears, due to the elasticity of the internal connection spring body, its slope lifting block will reset to close again.

[0011] Preferably, an inlet is provided at the upper side position of the placement heating box, and an internal heating tube body is provided at the internal position of the placement heating box.

[0012] Preferably, a connecting through pipe is provided at the side position of the placement heating box, an ultrafiltration filter column is provided at the bottom position of the connecting through pipe, an ultrafiltration membrane is provided at the internal position of the ultrafiltration filter column, and a driving water pump I and a connecting control solenoid valve II are provided at the bottom position of the ultrafiltration filter column.

[0013] Preferably, a chromatographic column is provided at the side position of the driving water pump I. The chromatographic column uses HILIC packing and uses a specific ratio of acetonitrile and water as the mobile phase. A connecting control solenoid valve I and a driving water pump II are provided at the side position of the chromatographic column.

[0014] Preferably, an operation control console is provided at the front side position of the ergothioneine extraction and purification equipment body, and the operation control console is installed and connected by a snap-in connection method.

[0015] Preferably, a data display screen is provided at the front side position of the operation control console. A protective film is pasted on the surface position of the data display screen, and operation control buttons are provided at the lower side position of the data display screen.

[0016] Preferably, bottom support universal wheels are provided at the bottom position of the ergothioneine extraction and purification equipment body, and the bottom support universal wheels are installed and connected to the bottom corner positions of the ergothioneine extraction and purification equipment body by rotating through threaded posts.

[0017] Compared with the prior art, the present utility model provides an automatic ergothioneine extraction and purification equipment, which has the following beneficial effects:

[0018] In the automatic ergothioneine extraction and purification equipment, an outer connection ring body is provided at the outer side position of the discharge port. An inclined surface lifting block is provided at the internal position of the outer connection ring body. An internal connection spring body is provided at the end position of the inclined surface lifting block. The outer connection ring body is rotationally connected to the discharge port by a thread. When in use, when the outer connection ring body is subjected to gas extrusion, the inclined surface position of its inclined surface lifting block will be squeezed and it will move to the side. When the extrusion force disappears, because of the elastic force of the internal connection spring body, its inclined surface lifting block will reset and close again. Through the self-sealing method of the present utility model, the discharge port can be effectively sealed after the product outflow stops, thereby effectively avoiding the phenomenon that the external environment contaminates the discharge port and affects the quality of the subsequent product. Description of the Drawings

[0019] Figure 1This is a schematic diagram of the overall structure of the automated ergothioneine extraction and purification equipment of the present utility model.

[0020] Figure 2 This is a schematic diagram of the overall sectional structure of the automated ergothioneine extraction and purification equipment of the present utility model.

[0021] Figure 3 This is an enlarged schematic diagram of the structure at position A of the automated ergothioneine extraction and purification equipment of the present utility model.

[0022] Figure 4 This is a sectional schematic diagram of the structure at position A of the automated ergothioneine extraction and purification equipment of the present utility model.

[0023] In the figure: 1. Ergothioneine extraction and purification equipment body; 2. Placing heating box; 3. Feeding port; 4. Connecting through pipe; 5. Operation control console; 6. Operation control button; 7. Data display screen; 8. Bottom support universal wheel; 9. Discharge port; 10. Ultrafiltration filter column; 11. Built-in heating pipe body; 12. Driving water pump 1; 13. Chromatographic column; 14. Outer connection ring body; 15. Slope lifting block; 16. Built-in connection spring body; 17. Connecting control solenoid valve 1; 18. Connecting control solenoid valve 2; 19. Driving water pump 2. Detailed implementation manners

[0024] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0025] The present utility model provides as Figures 1-4As shown in the figure, an automated ergothioneine extraction and purification device includes an ergothioneine extraction and purification device body 1 and a placement heating box 2 provided at the upper end of the ergothioneine extraction and purification device body 1; a discharge port 9 is provided at the right side position of the ergothioneine extraction and purification device body 1; an outer connection ring body 14 is provided at the outer side position of the discharge port 9, a slope lifting block 15 is provided at the inner position of the outer connection ring body 14, an internal connection spring body 16 is provided at the end position of the slope lifting block 15, and the outer connection ring body 14 is rotationally connected to the discharge port 9 by threads. During use, when the outer connection ring body 14 is subjected to gas extrusion, the slope position of its slope lifting block 15 will be squeezed and move to the side. When the extrusion force disappears, the internal connection spring body 16's elastic force will cause its slope lifting block 15 to reset and close. Through this utility model, the discharge port 9 can be effectively closed in a self-sealing manner after the product outflow stops, thereby effectively avoiding the phenomenon that the external environment contaminates the discharge port 9 and affects the quality of subsequent products.

[0026] As Figure 2 shown, an inlet 3 is provided at the upper side position of the placement heating box 2, an internal heating tube body 11 is provided at the inner position of the placement heating box 2, and the internal heating tube body 11 can heat the solution inside the placement heating box 2. The hot water is used to extract the mycelial fermentation broth containing ergothioneine to release ergothioneine into the aqueous solution. This step can help break the cell wall and make ergothioneine easier to extract.

[0027] As Figure 2 shown, a connecting through pipe 4 is provided at the side position of the placement heating box 2, an ultrafiltration filter column 10 is provided at the bottom position of the connecting through pipe 4, an ultrafiltration membrane is provided at the inner position of the ultrafiltration filter column 10, a driving water pump 12 and a connecting control solenoid valve 18 are provided at the bottom position of the ultrafiltration filter column 10. The ultrafiltration filter column 10 uses an ultrafiltration membrane with a cut-off molecular weight of 1 kDa to 30 kDa to perform ultrafiltration treatment on the ergothioneine aqueous solution to remove macromolecular impurities such as proteins and polysaccharides. The ultrafiltration permeate contains relatively pure ergothioneine.

[0028] As Figure 2 shown, a chromatographic column 13 is provided at the side position of the driving water pump 12. The chromatographic column 13 uses HILIC packing and uses a specific ratio of acetonitrile and water as the mobile phase. A connecting control solenoid valve 17 and a driving water pump 19 are provided at the side position of the chromatographic column 13. The chromatographic column 13 performs chromatographic purification on the ultrafiltration permeate to further improve the purity of ergothioneine.

[0029] As Figure 2As shown in the figure, an operation console 5 is provided at the front side of the ergothioneine extraction and purification equipment body 1. The operation console 5 is installed and connected by a snap-in plug-in method. A data display screen 7 is provided at the front side of the operation console 5. A protective film is pasted on the surface of the data display screen 7. Operation control buttons 6 are provided at the lower side of the data display screen 7. The operation console 5 can facilitate the staff to operate and control each component of the ergothioneine extraction and purification equipment body 1, so as to better extract and purify ergothioneine.

[0030] As Figure 1 and Figure 2 shown in the figure, bottom support universal wheels 8 are provided at the bottom position of the ergothioneine extraction and purification equipment body 1. The bottom support universal wheels 8 are installed and connected to the bottom corner positions of the ergothioneine extraction and purification equipment body 1 by rotating through threaded columns. The setting of the bottom support universal wheels 8 can reduce the friction between the ergothioneine extraction and purification equipment body 1 and the ground, facilitating the transfer of the ergothioneine extraction and purification equipment body 1 to a suitable position. At the same time, it can keep a certain distance between the ergothioneine extraction and purification equipment body 1 and the ground, reducing the degree of erosion on the bottom of the ergothioneine extraction and purification equipment body 1.

[0031] During operation, hot water is heated in the placement heating box 2 and then used to leach the mycelium fermentation broth containing ergothioneine with hot water to release ergothioneine into the aqueous solution. This step can help break the cell wall and make ergothioneine easier to extract. By driving the water pump 12, ergothioneine is pumped into the ultrafiltration column 10. The ultrafiltration column 10 uses an ultrafiltration membrane with a molecular weight cut-off of 1 kDa to 30 kDa to perform ultrafiltration treatment on the ergothioneine aqueous solution to remove macromolecular impurities such as proteins and polysaccharides. The ultrafiltration permeate contains relatively pure ergothioneine, and then it is pumped into the chromatographic column 13 by driving the water pump 19. The chromatographic column 13 performs chromatographic purification on the ultrafiltration permeate to further improve the purity of ergothioneine. Finally, the extracted ergothioneine is discharged and collected along the discharge port 9.

[0032] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An automated ergothioneine extraction and purification device, comprising an ergothioneine extraction and purification device body (1) and a placement heating box (2) arranged at the upper end of the ergothioneine extraction and purification device body (1); a discharge port (9) is arranged at the right side position of the ergothioneine extraction and purification device body (1); It is characterized in that: an outer connection ring body (14) is arranged at the outer side position of the discharge port (9), a slope lifting block (15) is arranged at the inner position of the outer connection ring body (14), an internal connection spring body (16) is arranged at the end position of the slope lifting block (15), the outer connection ring body (14) is rotationally connected to the discharge port (9) by threads. When in use, when the outer connection ring body (14) is squeezed by gas, the slope position of its slope lifting block (15) will be squeezed and cause it to move sideways. When the squeezing force disappears, the internal connection spring body (16) elasticity will cause its slope lifting block (15) to reset and close again.

2. The automated ergothioneine extraction and purification equipment according to claim 1, wherein: a placement port (3) is arranged at the upper side position of the placement heating box (2), and an internal heating tube body (11) is arranged at the inner position of the placement heating box (2).

3. An automated ergothioneine extraction and purification device according to claim 1, characterized in that: a connection through pipe (4) is arranged at the side position of the placement heating box (2), an ultrafiltration filter column (10) is arranged at the bottom position of the connection through pipe (4), an ultrafiltration membrane is arranged at the inner position of the ultrafiltration filter column (10), and a driving water pump one (12) and a connection control solenoid valve two (18) are arranged at the bottom position of the ultrafiltration filter column (10).

4. An automated ergothioneine extraction and purification device according to claim 3, characterized in that: a chromatographic column (13) is arranged at the side position of the driving water pump one (12), the chromatographic column (13) uses HILIC packing and uses acetonitrile and water as the mobile phase, and a connection control solenoid valve one (17) and a driving water pump two (19) are arranged at the side position of the chromatographic column (13).

5. An automated ergothioneine extraction and purification device according to claim 1, characterized in that: an operation control console (5) is arranged at the front side position of the ergothioneine extraction and purification device body (1), and the operation control console (5) is installed and connected by a snap-in plug-in method.

6. The automated ergothioneine extraction and purification equipment according to claim 5, characterized in that: a data display screen (7) is arranged at the front side position of the operation control console (5), a protective film is pasted on the surface position of the data display screen (7), and an operation control button (6) is arranged at the lower side position of the data display screen (7).

7. An automated ergothioneine extraction and purification device according to claim 1, characterized in that: bottom support universal wheels (8) are arranged at the bottom position of the ergothioneine extraction and purification device body (1), and the bottom support universal wheels (8) are installed and connected to the bottom corner positions of the ergothioneine extraction and purification device body (1) by rotating threaded columns.

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