Quantitative filling device for trace reagent in molecular biology
By designing a molecular biology micro reagent quantitative infusion device, the addition of reagents is automatically controlled, and the cumbersome problem of manual quantitative addition is solved, achieving the consistency of reagent dosage and the improvement of experimental efficiency.
Patent Information
- Application Number
- CN202422393516.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-30
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2034-09-30
AI Technical Summary
In molecular biology experiments, the prior art requires experimenters to manually add reagents, which is cumbersome and difficult to maintain consistency, affecting the experimental results.
Design a molecular biology micro reagent quantitative infusion device to automatically control the addition of reagents through the drive components and lift control module to ensure the consistency of each addition amount.
The reagent automation and quantitative addition are realized, the experimental efficiency is improved, the tedious process of manual operation is avoided, and the accuracy of experimental results is ensured.
Smart Images
Figure CN223113094U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of reagent filling, in particular to a quantitative filling device for trace reagents in molecular biology. Background Art
[0002] Molecular biology is a science that studies the structure and function of biological macromolecules at the molecular level to clarify the essence of life phenomena. Its main research fields include protein systems, protein-nucleic acid systems (with molecular genetics at the center), and protein-lipid systems (i.e., biological membranes). When conducting molecular biology experiments, in order to improve the accuracy of the experiments, several groups of control experiment examples are usually set for comparison;
[0003] In the prior art, when setting up control experiments, it is usually necessary to quantitatively add the reagent to be tested into the test tube. In actual application, it usually requires the experimenter to manually add it quantitatively based on the scale value, which is a relatively cumbersome process, and it is also difficult to keep the added amount consistent. Summary of the Utility Model
[0004] The purpose of the utility model is to provide a quantitative filling device for trace reagents in molecular biology, and solve the following technical problems:
[0005] When setting up control experiments, it is usually necessary to quantitatively add the reagent to be tested into the test tube. In actual application, it usually requires the experimenter to manually add it quantitatively based on the scale value, which is a relatively cumbersome process.
[0006] The purpose of the utility model can be realized by the following technical solutions:
[0007] A quantitative filling device for trace reagents in molecular biology includes a base, on which an experimental table is fixedly arranged. On one side of the experimental table, a carrier plate is rotatably arranged, and the carrier plate is fixedly connected to the output end of a driving motor fixedly arranged at the bottom of the experimental table. A plurality of positioning circular grooves for inserting test tubes are circumferentially arrayed on the carrier plate;
[0008] On the other side of the experimental table, there is a storage cylinder for storing experimental reagents; there is also a support shaft between the storage cylinder and the carrier plate, and the support shaft is connected to a rotating part for driving its rotation. Among them, on one side of the support shaft, there is a metering cylinder. At the end of the metering cylinder facing the experimental table, a piston is slidably arranged in the metering cylinder, and the piston is connected to a driving part for driving its lifting in the metering cylinder;
[0009] It also includes a lifting control module arranged on one side of the metering cylinder, and the lifting control module is used to adjust the rising height of the driving part driving the piston.
[0010] Preferably, the rotating part includes a toothed ring fixedly arranged on the outer edge surface of the carrier plate, and a gear fixedly arranged at the bottom of the support shaft for meshing with the toothed ring.
[0011] Preferably, a first electric cylinder is fixedly arranged on one side of the support shaft, and the driving end of the first electric cylinder is fixed to an adjusting plate slidably sleeved on the support shaft. Among them, the measuring cylinder is fixedly arranged on one side of the adjusting plate.
[0012] Preferably, the driving part includes a second electric cylinder fixedly arranged at the bottom of the adjusting plate. The driving end of the second electric cylinder is fixed to a lifting plate. A lifting rod is fixedly arranged on the piston, and the lifting rod slidably penetrates through the top wall of the measuring cylinder and is fixed to the lifting plate.
[0013] Preferably, the lifting control module includes scale lines arranged on the outer wall of the measuring cylinder. A positioning ring is also slidably sleeved on the outer wall of the measuring cylinder. A positioning bolt for abutting against the outer wall of the measuring cylinder is spirally arranged on the positioning ring. Among them, a measuring rod is fixedly arranged on one side of the lifting plate. A positioning plate is arranged at the bottom of the measuring rod. A control button is arranged on the positioning plate, and the control button is electrically connected to the second electric cylinder through a controller.
[0014] The beneficial effects of the present utility model are as follows:
[0015] (1) First, the molecular biology reagent to be experimented is stored in the storage cylinder. Secondly, the support shaft is driven to rotate through the rotating part so that the end can extend into the storage cylinder. The piston is driven to rise in the measuring cylinder through the driving part, and the reagent is sucked into the measuring cylinder under the action of negative pressure. After the suction is completed, the support shaft is driven to rotate again through the rotating part so that the measuring cylinder moves above the bearing plate, and the end extends into the test tube. Finally, the piston is driven to descend in the measuring cylinder through the driving part to discharge the reagent into the test tube; after a group of test tubes are added, the bearing plate is driven to rotate by the driving motor, and the above actions are repeated to add the reagent into the blank test tubes of this group. After the addition is completed, the experimenter can add another reagent to be detected into the test tube;
[0016] (2) By setting the lifting control module, each time the reagent is adsorbed from the storage cylinder, the lifting control module can adjust the height of the driving part to drive the piston to rise to be consistent, so that the amount of adsorbed reagent is consistent, achieving the effect of equal amount addition, avoiding the influence of inconsistent reagent amounts on the experimental results of molecular biology. Moreover, this process does not require manual operation, has a higher degree of automation, and improves the reagent addition efficiency. Description of the Drawings
[0017] The following further illustrates the present utility model with reference to the drawings.
[0018] Figure 1 is a schematic structural diagram of a molecular biology micro reagent quantitative dispenser of the present utility model Figure 1 ;
[0019] Figure 2 is a schematic structural diagram of a molecular biology micro reagent quantitative dispenser of the present utility model Figure 2 ;
[0020] Figure 3 It is a schematic structural diagram of a measuring cylinder in a micro reagent quantitative dispenser for molecular biology of the present utility model;
[0021] Figure 4 It is a schematic structural diagram of a piston in a micro reagent quantitative dispenser for molecular biology of the present utility model.
[0022] In the figure: 1, base; 2, driving motor; 3, test tube; 4, gear; 5, measuring cylinder; 101, experimental table; 102, storage cylinder; 201, bearing plate; 202, toothed ring; 203, positioning circular groove; 401, support shaft; 402, first electric cylinder; 403, adjusting plate; 404, lifting plate; 405, lifting rod; 406, second electric cylinder; 501, end; 502, scale line; 503, positioning plate; 504, button; 505, positioning ring; 506, positioning bolt; 507, measuring rod; 508, piston. Specific implementation manner
[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present utility model.
[0024] Embodiment 1
[0025] Please refer to Figures 1-4 As shown, the present utility model is a micro reagent quantitative dispenser for molecular biology, including a base 1. An experimental table 101 is fixedly arranged on the base 1. A bearing plate 201 is rotatably arranged on one side of the experimental table 101. The bearing plate 201 is fixedly connected to the output end of a driving motor 2 fixedly arranged at the bottom of the experimental table 101. A plurality of groups of positioning circular grooves 203 for inserting test tubes 3 are circumferentially arranged on the bearing plate 201; In an implementation manner of this embodiment, five groups of positioning circular grooves 203 are provided;
[0026] On the other side of the experimental bench 101, there is a storage cylinder 102 for storing experimental reagents; a support shaft 401 is also provided between the storage cylinder 102 and the carrier plate 201, and the support shaft 401 is connected to a rotating part that drives its rotation. Among them, on one side of the support shaft 401, there is a measuring cylinder 5, and the layout end 501 of the measuring cylinder 5 faces the experimental bench 101. A piston 508 is slidably arranged in the measuring cylinder 5, and the piston 508 is connected to a driving part that drives its lifting in the measuring cylinder 5; specifically, in this embodiment, first, the molecular biology reagent to be experimented is stored in the storage cylinder 102. Secondly, the support shaft 401 is driven to rotate by the rotating part so that the end 501 can extend into the storage cylinder 102. The piston 508 is driven to rise in the measuring cylinder 5 by the driving part, and the reagent is sucked into the measuring cylinder 5 under the action of negative pressure. After the suction is completed, the support shaft 401 is driven to rotate again by the rotating part so that the measuring cylinder 5 moves above the carrier plate 201, and the end 501 extends into the test tube 3. Finally, the piston 508 is driven to descend in the measuring cylinder 5 by the driving part to discharge the reagent into the test tube 3; after a group of test tubes 3 are added, the carrier plate 201 is driven to rotate by the driving motor 2, and the above actions are repeated to add the reagent into the blank test tubes 3 of this group. After the addition is completed, the experimenter can add another reagent to be detected into the test tube 3.
[0027] It also includes a lifting control module arranged on one side of the measuring cylinder 5. The lifting control module is used to adjust the rising height of the driving part driving the piston 508; it can be explained that in this embodiment, by setting the lifting control module, each time the reagent is adsorbed from the storage cylinder 102, the lifting control module can adjust the rising height of the driving part driving the piston 508 to be consistent, so that the adsorbed reagent amount is consistent, achieving the effect of equal addition, avoiding the influence of inconsistent reagent amounts on the experimental results of molecular biology. Moreover, this process does not require manual operation, has a higher degree of automation, and improves the reagent addition efficiency.
[0028] Embodiment 2
[0029] On the basis of Embodiment 1, the rotating part includes a toothed ring 202 fixedly arranged on the outer edge surface of the carrier plate 201, and a gear 4 is fixedly arranged at the bottom of the support shaft 401 for meshing with the toothed ring 202; it can be explained that when the driving motor 2 drives the carrier plate 201 to rotate, the toothed ring 202 can be driven to rotate synchronously. When the toothed ring 202 rotates, it drives the support shaft 401 to rotate by meshing with the gear 4;
[0030] It should be noted that in this embodiment, after a group of test tubes 3 are added with reagents, when the carrier plate 201 rotates to drive the next group of test tubes 3 to move to the reagent addition position, the toothed ring 202 can drive the gear 4 to just rotate 360 degrees. Correspondingly, when the gear 4 rotates 180 degrees, the end 501 is just located above the storage cylinder 102. At this time, the carrier plate 201 stops rotating and waits for the reagent suction to be completed, then rotates 180 degrees and resets to the reagent addition position.
[0031] Please refer to Figures 2-4 , on one side of the support shaft 401, a first electric cylinder 402 is fixedly arranged. The driving end of the first electric cylinder 402 is fixed to an adjusting plate 403 that is slidably sleeved on the support shaft 401. Among them, the measuring cylinder 5 is fixedly arranged on one side of the adjusting plate 403. It can be explained that when the measuring cylinder 5 sucks or discharges the reagent, the first electric cylinder 402 drives the adjusting plate 403 to move up and down on the support shaft 401. During the up and down movement of the adjusting plate 403, the measuring cylinder 5 can be synchronously driven to move up and down to adjust the height of the end 501, so as to facilitate sucking the reagent or discharging the reagent into the test tube 3.
[0032] The driving part includes a second electric cylinder 406 fixedly arranged at the bottom of the adjusting plate 403. The driving end of the second electric cylinder 406 is fixed to a lifting plate 404. A lifting rod 405 is fixedly arranged on the piston 508. The lifting rod 405 slidably penetrates the top wall of the measuring cylinder 5 and is fixed to the lifting plate 404. It can be explained that in this embodiment, when sucking or discharging the reagent, the second electric cylinder 406 drives the lifting rod 405 to move up and down through the lifting plate 404, and the lifting rod 405 can drive the piston 508 to move up and down in the measuring cylinder 5.
[0033] The lifting control module includes scale lines 502 arranged on the outer wall of the measuring cylinder 5. A positioning ring 505 is also slidably sleeved on the outer wall of the measuring cylinder 5. A positioning bolt 506 for abutting against the outer wall of the measuring cylinder 5 is spirally arranged on the positioning ring 505. Among them, a measuring rod 507 is fixedly arranged on one side of the lifting plate 404. A positioning plate 503 is arranged at the bottom of the measuring rod 507. A control button 504 is arranged on the positioning plate 503. The control button 504 is electrically connected to the second electric cylinder 406 through a controller. It can be explained that before taking the reagent, the experimenter first adjusts the position height of the positioning ring 505 on the measuring cylinder 5 based on the amount to be taken. After the adjustment is completed, the positioning bolt 506 is tightened to position the positioning ring 505. When the second electric cylinder 406 drives the lifting plate 404 to rise, the measuring rod 507 can be synchronously driven to rise. The measuring rod 507 drives the positioning plate 503 to rise. When the control button 504 abuts against the positioning ring 505, it contracts inward and generates an electric signal. The electric signal controls the second electric cylinder 406 to stop moving through the controller, and the reagent extraction is completed.
[0034] The principle of the present utility model is as follows: First, store the molecular biology reagents to be experimented in the storage cylinder 102. Secondly, drive the support shaft 401 to rotate through the rotating part so that the end 501 can extend into the storage cylinder 102. Drive the piston 508 to rise in the metering cylinder 5 through the driving part, and suck the reagent into the metering cylinder 5 under the action of negative pressure. After the suction is completed, drive the support shaft 401 to rotate again through the rotating part so that the metering cylinder 5 moves above the bearing plate 201, extend the end 501 into the test tube 3, and finally drive the piston 508 to descend in the metering cylinder 5 to discharge the reagent into the test tube 3. After a group of test tubes 3 are filled, drive the bearing plate 201 to rotate through the drive motor 2, repeat the above actions to add the reagent into the blank test tubes 3 of this group. After the addition is completed, the experimenter can add another reagent to be detected into the test tube 3. Among them, before taking the reagent, the experimenter first adjusts the position height of the positioning ring 505 on the metering cylinder 5 based on the amount to be taken. After the adjustment is completed, tighten the positioning bolt 506 to position the positioning ring 505. When the second electric cylinder 406 drives the lifting plate 404 to rise, it can synchronously drive the metering rod 507 to rise. The metering rod 507 drives the positioning plate 503 to rise. When the control button 504 abuts against the positioning ring 505, it contracts inward and generates an electric signal. The electric signal controls the second electric cylinder 406 to stop moving through the controller, and the reagent extraction is completed.
[0035] In the description of the present utility model, it should be understood that the terms "upper", "lower", "left", "right", etc. indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, as well as a specific orientation structure and operation. Therefore, it cannot be understood as a limitation to the present utility model. In addition, "first" and "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Therefore, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, unless otherwise specified, the meaning of "a plurality" is two or more.
[0036] In the description of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection", "connection", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.
[0037] The above has described in detail an embodiment of the present utility model. However, the above content is only a preferred embodiment of the present utility model and should not be construed as limiting the scope of implementation of the present utility model. Equal changes and improvements made in accordance with the scope of application of the present utility model should still fall within the scope covered by the patent of the present utility model.
Claims
1. A micro reagent quantitative dispenser for molecular biology, comprising a base (1), characterized in that, An experimental bench (101) is fixedly arranged on a base (1). A bearing disc (201) is rotatably arranged on one side of the experimental bench (101). The bearing disc (201) is fixedly connected to the output end of a driving motor (2) fixedly arranged at the bottom of the experimental bench (101). A plurality of groups of positioning circular grooves (203) for inserting test tubes (3) are circumferentially arrayed on the bearing disc (201). On the other side of the experimental bench (101), there is a storage cylinder (102) for storing experimental reagents. There is also a support shaft (401) between the storage cylinder (102) and the bearing disc (201). The support shaft (401) is connected to a rotating part that drives its rotation. Among them, on one side of the support shaft (401), there is a measuring cylinder (5). The arranging end (501) of the measuring cylinder (5) faces the direction of the experimental bench (101). A piston (508) is slidably arranged in the measuring cylinder (5). The piston (508) is connected to a driving part that drives it to lift and lower in the measuring cylinder (5). It also includes a lifting control module arranged on one side of the measuring cylinder (5). The lifting control module is used to adjust the rising height of the driving part driving the piston (508).
2. The micro reagent quantitative dispenser for molecular biology according to claim 1, wherein The rotating part includes a toothed ring (202) fixedly arranged on the outer edge surface of the bearing disc (201). A gear (4) for meshing with the toothed ring (202) is fixedly arranged at the bottom of the support shaft (401).
3. A micro reagent quantitative filling device for molecular biology according to claim 1, characterized in that, A first electric cylinder (402) is fixedly arranged on one side of the support shaft (401). The driving end of the first electric cylinder (402) is fixedly connected to an adjusting plate (403) slidably sleeved on the support shaft (401). Among them, the measuring cylinder (5) is fixedly arranged on one side of the adjusting plate (403).
4. A micro reagent quantitative dispenser for molecular biology according to claim 3, characterized in that, The driving part includes a second electric cylinder (406) fixedly arranged at the bottom of the adjusting plate (403). The driving end of the second electric cylinder (406) is fixedly connected to a lifting plate (404). A lifting rod (405) is fixedly arranged on the piston (508). The lifting rod (405) slidably penetrates the top wall of the measuring cylinder (5) and is fixedly connected to the lifting plate (404).
5. The micro reagent quantitative dispenser for molecular biology according to claim 4, characterized in that The lifting control module includes scale lines (502) arranged on the outer wall of the measuring cylinder (5). A positioning ring (505) is also slidably sleeved on the outer wall of the measuring cylinder (5). A positioning bolt (506) for abutting against the outer wall of the measuring cylinder (5) is spirally arranged on the positioning ring (505). Among them, a measuring rod (507) is fixedly arranged on one side of the lifting plate (404). A positioning plate (503) is arranged at the bottom of the measuring rod (507). A control button (504) is arranged on the positioning plate (503). The control button (504) is electrically connected to the second electric cylinder (406) through a controller.