Automatic cell culture device

Abstract

The utility model relates to the technical field of cell culture, and discloses an automatic cell culture device which comprises a culture box body and a sealing device, a storage device is installed in the middle of the sealing device, a second supporting frame is connected to the surface of the bottom end of the storage device, and a metal frame is in threaded connection with the outward end of the second supporting frame. And the sealing device is arranged at the upper end of the incubator body. According to the automatic cell culture device, the limiting assembly, the liquid storage tank and the sealing device are arranged, and the limiting device can fix the liquid storage tank and the limiting device, so that collision between the liquid storage tank and the sealing device can be prevented, the safety of the liquid storage tank can be protected, and meanwhile, the liquid storage tank can be prevented from moving up and down in the sealing device; the hexagon nuts are rotated at the upper ends of the L-shaped clamping plates, the hexagon nuts can fix the movable rolling wheels and the L-shaped clamping plates in a threaded mode, the L-shaped clamping plates of different specifications can be used according to the sealing device, and therefore the liquid storage tank and the sealing device can be limited and fixed.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an automatic cell culture device. Background Technique

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture technology can grow a single cell into a simple single cell or a multicellular with very little differentiation through a large amount of culture. This is an essential link in cloning technology, and cell culture itself is cell cloning.

[0003] In the prior art, the utility model with the patent number 202122796744.7 discloses an automatic cell culture device, which includes an incubator, a placement component, an adding component and an intermittent indexing mechanism; a placement component is installed in the incubator, and the placement component includes a support column and a placement disk surrounding the support column; an adding component is installed on the support column, and the adding component includes an installation cylinder, the installation cylinder is coaxially rotatably connected to the support column, and an intermittent indexing mechanism is installed at the connection between the installation cylinder and the support column; a first-stage hydraulic rod is rotatably installed in the installation cylinder, the first-stage hydraulic rod is rotatably connected to a control rod, the end of the control rod away from the first-stage hydraulic rod passes through the installation cylinder and is connected to a second-stage hydraulic rod, and the second-stage hydraulic rod is rotatably connected to an adding head. This device can automatically and accurately add nutrients to all cultures, improving efficiency, reducing the opening of the culture device, and reducing the probability of contamination.

[0004] In the above-mentioned incubator, a liquid storage tank is installed. When replacing the liquid storage tank, the liquid storage tank is directly pulled out. However, the liquid storage tank is not limited. When the incubator vibrates, the liquid storage tank inside the incubator will move up and down, resulting in a collision between the liquid storage tank and the incubator, damaging the liquid storage tank, causing the nutrient solution inside the liquid storage tank to flow out, and unable to effectively carry out the culture operation. Content of the Utility Model

[0005] The purpose of the utility model is to provide an automatic cell culture device to solve the problems raised in the above background technique.

[0006] To achieve the above object, the utility model provides the following technical solutions: an automatic cell culture device, which includes a culture box body and a sealing device. A storage device is installed at the middle position of the sealing device. A second support frame is connected to the bottom surface of the storage device. The outer end of the second support frame is threadedly connected to a metal frame. The sealing device is installed at the upper end of the culture box body. The storage device includes a liquid storage box body, a limiting component, a syringe, and a transportation pipeline. The syringe is located at the lower end of the storage device. The syringe is installed on the bottom surface of the transportation pipeline. The transportation pipeline is fixedly connected to the bottom surface of the liquid storage box body. The limiting component is threadedly connected to the upper end of the liquid storage box body.

[0007] Preferably, the limiting component includes an L-shaped clamping plate, a sliding frame, a moving roller, a first support frame, a plug-in threaded rod, and a spiral spring. The moving roller is located at the front end of the limiting component. The moving roller is threadedly connected to the upper end of the L-shaped clamping plate. The moving roller is movably connected to the outer end of the sliding frame. The sliding frame is movably connected to the front end of the plug-in threaded rod. The sliding frame is fixedly connected to the front of the spiral spring. The plug-in threaded rod is fixedly connected to the middle position of the first support frame.

[0008] Preferably, the liquid storage box body is movably connected to the middle position of the sealing device. The metal frame is inserted into the middle position of the sealing device. The second support frame is movably connected to the bottom surface of the liquid storage box body. Rotate the nut, and the nut rotates at the lower end of the metal frame, so that the second support frame and the metal frame can be threadedly fixed, and thus the position of the liquid storage box body can be determined.

[0009] Preferably, the L-shaped clamping plate is movably connected to the front end of the sealing device and the top surface of the sealing device. The L-shaped clamping plate drives the moving roller to move on the inner wall surface of the sliding frame, and the L-shaped clamping plate is inserted into the front end of the sealing device, so that the sealing device and the liquid storage box body can be inserted and limited.

[0010] Preferably, the first support frame is threadedly connected to the top surface of the liquid storage box body. A fixed threaded rod is provided on the top surface of the liquid storage box body. The fixed threaded rod penetrates through the first support frame, and then rotate the nut, and the nut can threadedly fix the first support frame and the liquid storage box body, so that the position of the first support frame can be determined.

[0011] Preferably, the sliding frame is fixedly connected to the front of the spiral spring. The plug-in threaded rod is located inside the spiral spring. Rotate the hexagonal nut at the front end of the plug-in threaded rod, and the hexagonal nut can push the sliding frame to move on the threaded end surface of the plug-in threaded rod, so that the position of the sliding frame can be adjusted.

[0012] Preferably, the number of the sliding frames is two, and the two sliding frames are distributed front and back. The two sliding frames can determine the position of the L-shaped clamping plate and limit and fix the position of the liquid storage box body.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] 1. A limiting component, a liquid storage box and a sealing device are provided. The limiting device can limit and fix the liquid storage box, thereby preventing the liquid storage box and the sealing device from colliding, protecting the safety of the liquid storage box, and at the same time preventing the liquid storage box from moving up and down inside the sealing device, which can increase the service life of the device;

[0015] 2. A sliding frame, a moving roller and an L-shaped clamping plate are provided. By rotating the hexagon nut at the upper end of the L-shaped clamping plate, the hexagon nut can thread-fix the moving roller and the L-shaped clamping plate, and L-shaped clamping plates of different specifications can be used according to the sealing device, so as to limit and fix the liquid storage box and the sealing device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is an overall three-dimensional schematic diagram of the present utility model;

[0017] Figure 2 is a three-dimensional schematic diagram of the storage device, the metal frame and the support two of the present utility model;

[0018] Figure 3 is a three-dimensional schematic diagram of the limiting component of the present utility model;

[0019] Figure 4 is the present utility model Figure 3 Partial enlarged schematic diagram of the structure at A in;

[0020] Figure 5 is the present utility model Figure 3 Partial enlarged schematic diagram of the structure at B in.

[0021] In the figure: 1. Storage device; 11. Liquid storage box body; 12. Limiting component; 121. L-shaped clamping plate; 122. Sliding frame; 123. Moving roller; 124. Support frame one; 125. Inserted threaded rod; 126. Helical spring; 13. Injector; 14. Transportation pipeline; 2. Culture box; 3. Sealing device; 4. Metal frame; 5. Support frame two. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without making creative efforts shall fall within the protection scope of the present utility model.

[0023] Please refer to Figures 1-5 , the present utility model provides the following technical solutions:

[0024] An automated cell culture device includes a culture box body 2 and a sealing device 3. A storage device 1 is installed at the middle position of the sealing device 3. A support frame two 5 is connected to the bottom surface of the storage device 1. The outer end of the support frame two 5 is threadedly connected to a metal frame 4. The sealing device 3 is installed at the upper end of the culture box body 2.

[0025] The storage device 1 includes a liquid storage box body 11, a limiting component 12, a syringe 13 and a transport pipeline 14. The syringe 13 is located at the lower end of the storage device 1. The syringe 13 is installed on the bottom surface of the transport pipeline 14. The transport pipeline 14 is fixedly connected to the bottom surface of the liquid storage box body 11. The liquid storage box body 11 is movably connected to the middle position of the sealing device 3. The metal frame 4 is inserted into the middle position of the sealing device 3. The support frame two 5 is movably connected to the bottom surface of the liquid storage box body 11. Rotate the nut, and the nut rotates at the lower end of the metal frame 4, so that the support frame two 5 and the metal frame 4 can be threadedly fixed, and thus the position of the liquid storage box body 11 can be determined. The limiting component 12 is threadedly connected to the upper end of the liquid storage box body 11.

[0026] The limiting component 12 includes an L-shaped clamping plate 121, a sliding frame 122, a moving roller 123, a first support frame 124, a plug-in threaded rod 125 and a spiral spring 126. The moving roller 123 is located at the front end of the limiting component 12. The moving roller 123 is threadedly connected to the upper end of the L-shaped clamping plate 121. The L-shaped clamping plate 121 is movably connected to the front end of the sealing device 3 and the top surface of the sealing device 3. The L-shaped clamping plate 121 drives the moving roller 123 to move on the inner wall surface of the sliding frame 122, so that the L-shaped clamping plate 121 is inserted into the front end of the sealing device 3, thereby enabling the sealing device 3 and the liquid storage box 11 to be inserted and limited. The moving roller 123 is movably connected to the outer end of the sliding frame 122. The sliding frame 122 is fixedly connected to the front of the spiral spring 126. The plug-in threaded rod 125 is located inside the spiral spring 126. By rotating the hexagonal nut at the front end of the plug-in threaded rod 125, the hexagonal nut can push the sliding frame 122 to move on the threaded end surface of the plug-in threaded rod 125, thereby enabling the position of the sliding frame 122 to be adjusted. The sliding frame 122 is movably connected to the front end of the plug-in threaded rod 125. The number of sliding frames 122 is two. The two sliding frames 122 are distributed front and back. The two sliding frames 122 can determine the position of the L-shaped clamping plate 121 and can limit and fix the position of the liquid storage box 11. The sliding frame 122 is fixedly connected to the front of the spiral spring 126. The plug-in threaded rod 125 is fixedly connected to the middle position of the first support frame 124. The first support frame 124 is threadedly connected to the top surface of the liquid storage box 11. A fixed threaded rod is provided on the top surface of the liquid storage box 11. The fixed threaded rod passes through the first support frame 124, and then by rotating the nut, the nut can threadedly fix between the first support frame 124 and the liquid storage box 11, thereby enabling the position of the first support frame 124 to be determined.

[0027] During use, push the metal frame 4 downward so that the metal frame 4 is inserted into the middle position of the sealing device 3, and then push the second support frame 5 upward so that the metal frame 4 and the second support frame 5 are threadedly connected, thereby enabling the position of the metal frame 4 to be determined.

[0028] Push the sealing device 3 downward. The sealing device 3 is inserted into the upper end of the culture box 2, thereby enabling the position of the sealing device 3 to be determined.

[0029] Push the liquid storage box 11 downward so that the liquid storage box 11 is inserted into the middle position of the sealing device 3, and the bottom surface of the liquid storage box 11 is connected to the top surface of the second support frame 5, thereby enabling the position of the liquid storage box 11 to be determined.

[0030] Push the support frame 124 downward so that the upper end of the liquid storage box 11 penetrates through the support frame 124, connect the bottom surface of the support frame 124 to the top surface of the liquid storage box 11, and then rotate the nut, which can thread-fix the support frame 124 and the liquid storage box 11, thereby thread-fixing the support frame 124 and the liquid storage box 11.

[0031] Push the L-shaped clamping plate 121 upward so that the upper end of the L-shaped clamping plate 121 penetrates through the upper end of the moving roller 123, thereby initially determining the position between the sliding frame 122 and the L-shaped clamping plate 121.

[0032] Rotate the hexagon nut, which rotates at the upper end of the L-shaped clamping plate 121, thereby initially determining the position between the L-shaped clamping plate 121 and the moving roller 123.

[0033] Push the sliding frame 122 to move back and forth. The sliding frame 122 slides into the front end of the support frame 124, enabling the support frame 124 to slide at the front end of the inserted threaded rod 125. Let the sliding frame 122 push the helical spring 126 backward, connecting the back surface of the helical spring 126 to the middle of the support frame 124.

[0034] Rotate the hexagon nut at the front end of the inserted threaded rod 125. The hexagon nut can push the sliding frame 122 to move on the threaded end surface of the inserted threaded rod 125. At the same time, the helical spring 126 can be compressed between the sliding frame 122 and the support frame 124, thereby adjusting the position of the sliding frame 122.

[0035] Push the L-shaped clamping plate 121 to move left and right, insert the L-shaped clamping plate 121 into the front end and the rear end of the sealing device 3, thereby inserting and fixing the liquid storage box 11 and the sealing device 3, and then determining the position of the liquid storage box 11, making the replacement of the liquid storage box 11 more convenient and fast.

[0036] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to the embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automated cell culture device, comprising a culture chamber (2) and a sealing device (3), characterized in that: A storage device (1) is installed at the middle position of the sealing device (3). A second support frame (5) is connected to the bottom surface of the storage device (1). The outer end of the second support frame (5) is threadedly connected to a metal frame (4). The sealing device (3) is installed at the upper end of the culture box body (2). The storage device (1) includes a liquid storage box body (11), a limiting component (12), a liquid injector (13) and a transportation pipeline (14). The liquid injector (13) is located at the lower end of the storage device (1). The liquid injector (13) is installed on the bottom surface of the transportation pipeline (14). The transportation pipeline (14) is fixedly connected to the bottom surface of the liquid storage box body (11). The limiting component (12) is threadedly connected to the upper end of the liquid storage box body (11).

2. An automated cell culture device according to claim 1, characterized in that: The limiting component (12) includes an L-shaped clamping plate (121), a sliding frame (122), a moving roller (123), a first support frame (124), a plug-in threaded rod (125) and a spiral spring (126). The moving roller (123) is located at the front end of the limiting component (12). The moving roller (123) is threadedly connected to the upper end of the L-shaped clamping plate (121). The moving roller (123) is movably connected to the outer end of the sliding frame (122). The sliding frame (122) is movably connected to the front end of the plug-in threaded rod (125). The sliding frame (122) is fixedly connected to the front of the spiral spring (126). The plug-in threaded rod (125) is fixedly connected to the middle position of the first support frame (124).

3. An automated cell culture device according to claim 2, characterized in that: The liquid storage box body (11) is movably connected to the middle position of the sealing device (3). The metal frame (4) is inserted into the middle position of the sealing device (3). The second support frame (5) is movably connected to the bottom surface of the liquid storage box body (11).

4. An automated cell culture device according to claim 3, characterized in that: The L-shaped clamping plate (121) is movably connected to the front end of the sealing device (3). The L-shaped clamping plate (121) is movably connected to the top surface of the sealing device (3).

5. An automated cell culture device according to claim 4, characterized in that: The first support frame (124) is threadedly connected to the top surface of the liquid storage box body (11).

6. An automated cell culture device according to claim 5, characterized in that: The plug-in threaded rod (125) is located inside the spiral spring (126).

7. An automated cell culture device according to claim 6, characterized in that: The number of the sliding frames (122) is two, and the two sliding frames (122) are distributed front and back.

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