Immunolabeling instrument capable of conveniently adjusting reagent amount

Abstract

The utility model discloses an immunolabeling instrument convenient to adjust reagent amount, including refrigeration bottom cabinet, equipment top box and perfusion device, refrigeration bottom cabinet top center position is provided with the rotary connection disc, refrigeration bottom cabinet top far away from the cabinet door side is connected with the connection side plate, equipment top box is connected with the connection side plate top, and the perfusion device is connected with the equipment top box. A reagent supplementing tank is connected to the side, away from the rotary connecting disc, of the connecting side plate, a liquid inlet is formed in the top of the reagent supplementing tank, the filling device is connected to the bottom of the equipment top box and comprises a filling needle and a connecting hose, and the filling needle is connected to the bottom of the connecting hose. An injection hose is arranged in the connecting hose, one end of the injection hose is connected with a perfusion needle, a tracer agent can be automatically injected, the injection dosage of the tracer agent can be automatically controlled, and the immunofluorescence labeling technology is more automatic and controllable.

Description

Technical Field

[0001] The utility model relates to the technical field of immune labelers, in particular to an immune labeler which is convenient for adjusting the reagent dosage. Background Art

[0002] The biomedical industry often needs to perform operations such as immune labeling on cells. Immune labeling refers to the antigen-antibody reaction in which antibodies or antigens are labeled with fluorescein, enzymes, radioisotopes, or electron-dense substances, etc., which has high sensitivity and specificity and has been widely used.

[0003] Due to different methods adopted, immune labeling techniques are mainly divided into: immunofluorescence technique, radioimmunoassay, and enzyme-labeling technique. Among them, immunofluorescence and radioimmunoassay are more widely used. However, immunofluorescence is time-consuming, cannot be quantified and automated, and cannot control the dosage of the tracer.

[0004] Therefore, in view of the existing structures and deficiencies, research and improvement are carried out, and an immune labeler which is convenient for adjusting the reagent dosage is proposed, in order to achieve a more practical value. Content of the Utility Model

[0005] The purpose of the utility model is to provide an immune labeler which is convenient for adjusting the reagent dosage, so as to solve the problems in the above background that the immunofluorescence technique cannot be quantified and automated, and cannot control the dosage of the tracer.

[0006] To achieve the above purpose, the utility model provides the following technical solution: an immune labeler which is convenient for adjusting the reagent dosage, including a refrigerated bottom cabinet, an equipment top box, and a perfusion device. A cabinet door is arranged on one side of the refrigerated bottom cabinet. A rotary connection disk is arranged at the center of the top of the refrigerated bottom cabinet. A connecting side plate is connected to the top of the refrigerated bottom cabinet on the side far from the cabinet door. The equipment top box is connected to the top of the connecting side plate. A reagent replenishment tank is connected to the side of the connecting side plate far from the rotary connection disk. A liquid inlet is arranged at the top of the reagent replenishment tank. The perfusion device is connected to the bottom of the equipment top box. The perfusion device includes a perfusion needle and a connecting hose. The perfusion needle is connected to the bottom of the connecting hose. An injection hose is arranged inside the connecting hose. One end of the injection hose is connected to the perfusion needle.

[0007] Preferably, a perfusion lifting cylinder is arranged on one side of the perfusion device. A lifting connecting rod is connected between the connecting hose and the perfusion lifting cylinder. Connecting collar rings are arranged at the joints of the lifting connecting rod with the connecting hose and the perfusion lifting cylinder.

[0008] Preferably, a reagent storage tank, a liquid pushing controller and a liquid pushing cylinder are arranged inside the top box of the device, and the liquid pushing cylinder is arranged between the reagent storage tank and the liquid pushing controller. A liquid level gauge is arranged inside the reagent storage tank.

[0009] Preferably, a supplementary water pump is arranged inside the reagent supplementary tank, and a water suction pipe is connected to the bottom of the supplementary water pump. A supplementary water pipe is connected between the reagent storage tank and the supplementary water pump, and the liquid level gauge is connected to the supplementary water pump.

[0010] Preferably, a syringe is arranged below the liquid pushing cylinder. An injection head is connected to the bottom of the syringe, and one end of the injection hose far away from the perfusion needle is connected to the injection head. A connecting water pipe is connected between the reagent storage tank and the syringe.

[0011] Preferably, a liquid pushing rod is arranged inside the liquid pushing cylinder, and a liquid pushing piston is connected to one end of the liquid pushing rod far away from the liquid pushing cylinder. A connecting air pipe is connected between the liquid pushing controller and the liquid pushing cylinder, and an air inlet pipe is connected to one side of the liquid pushing controller far away from the connecting air pipe.

[0012] Compared with the prior art, the beneficial effects of the present utility model are as follows: the tracer can be automatically injected, and the injection dose of the tracer can be automatically controlled, making the immunofluorescence labeling technology more automated and controllable. The specific content is as follows:

[0013] The cell container to be detected is placed in the rotary connecting disc, and the rotary connecting disc drives the cell container to rotate and move. The tracer in the reagent storage tank enters the syringe through the connecting water pipe, and the syringe injects the tracer into the injection hose through the injection head. The liquid pushing controller is connected to the gas storage tank through the air inlet pipe, and the liquid pushing controller can control the telescopic length of the liquid pushing rod in the liquid pushing cylinder by intelligently controlling the equivalent of the input gas of the connecting air pipe, and the tracer in the syringe is pushed out by the injection head through the extrusion of the liquid pushing piston, so as to control the amount of the pushed and injected tracer. The perfusion device is driven by the perfusion lifting cylinder to lift, so that the perfusion needle is close to or far away from the cell container, so that the perfusion work can be carried out continuously for multiple times. The liquid level gauge detects the liquid level height of the tracer in the reagent storage tank, and controls the supplementary water pump to supplement the tracer into the reagent storage tank in time according to the data transmitted by the liquid level gauge. Description of the Drawings

[0014] Figure 1 It is a schematic structural diagram of the main body of an immunolabeling instrument for conveniently adjusting the reagent amount according to the present utility model;

[0015] Figure 2 It is a schematic structural diagram of the lifting structure of the perfusion device of an immunolabeling instrument for conveniently adjusting the reagent amount according to the present utility model;

[0016] Figure 3 Schematic diagram of the side section of the equipment top box of an immunolabeling instrument for facilitating the adjustment of reagent dosage according to the present utility model;

[0017] Figure 4 Schematic diagram of the quantitative perfusion structure of an immunolabeling instrument for facilitating the adjustment of reagent dosage according to the present utility model.

[0018] In the figure: 1, refrigerated bottom cabinet; 11, cabinet door; 2, rotating connection disk; 3, connecting side plate; 4, equipment top box; 41, reagent storage tank; 411, liquid level gauge; 412, connecting water pipe; 42, liquid pushing controller; 421, connecting air pipe; 422, inlet air pipe; 43, liquid pushing cylinder; 431, liquid pushing rod; 432, liquid pushing piston; 44, syringe; 441, injection head; 5, perfusion device; 51, perfusion needle; 52, connecting hose; 53, injection hose; 6, reagent replenishment tank; 61, liquid inlet; 62, replenishment water pump; 621, replenishment water pipe; 63, water suction pipe; 7, perfusion lifting cylinder; 71, lifting connecting rod; 711, connecting collar. Specific embodiments

[0019] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0020] Please refer to Figures 1 to 4 , the present utility model provides a technical solution of an immunolabeling instrument for facilitating the adjustment of reagent dosage, including a refrigerated bottom cabinet 1, an equipment top box 4 and a perfusion device 5. A cabinet door 11 is arranged on one side of the refrigerated bottom cabinet 1. A rotating connection disk 2 is arranged at the central position of the top of the refrigerated bottom cabinet 1. And a connecting side plate 3 is connected to the top of the refrigerated bottom cabinet 1 on the side far from the cabinet door 11. The equipment top box 4 is connected to the top of the connecting side plate 3. A reagent replenishment tank 6 is connected to the side of the connecting side plate 3 far from the rotating connection disk 2. And a liquid inlet 61 is arranged at the top of the reagent replenishment tank 6. The perfusion device 5 is connected to the bottom of the equipment top box 4. The perfusion device 5 includes a perfusion needle 51 and a connecting hose 52. And the perfusion needle 51 is connected to the bottom of the connecting hose 52. An injection hose 53 is arranged inside the connecting hose 52. And one end of the injection hose 53 is connected to the perfusion needle 51.

[0021] The refrigerated floor cabinet 1 is used to place various reagents and containers for labeled cells, facilitating access and refrigerated storage of the reagents and containers for labeled cells. The refrigerated floor cabinet 1 can be opened and closed through the cabinet door 11. The equipment top box 4 and the reagent replenishment tank 6 are connected to the refrigerated bottom cabinet 1 through the connecting side plate 3. The reagent replenishment tank 6 contains the replenished tracer, and the tracer can be added to the reagent replenishment tank 6 through the liquid inlet 61. The rotary connection disk 2 is used to place the cell containers to be detected, and the perfusion device 5 injects the tracer into the cell containers through the perfusion needle 51.

[0022] On one side of the perfusion device 5, there is a perfusion lifting cylinder 7. A lifting connecting rod 71 is connected between the connecting hose 52 and the perfusion lifting cylinder 7. Connection collars 711 are provided at the connection points of the lifting connecting rod 71 with the connecting hose 52 and the perfusion lifting cylinder 7. The perfusion device 5 is driven by the perfusion lifting cylinder 7 to move up and down, so that the perfusion needle 51 approaches or moves away from the cell containers, enabling continuous perfusion operations multiple times. The perfusion lifting cylinder 7 drives the lifting connecting rod 71 to move up and down, and drives the connecting hose 52 to expand and contract through the connection collar 711. During the expansion and contraction of the connecting hose 52, the connecting hose 52 is pulled.

[0023] Inside the equipment top box 4, there are a reagent storage tank 41, a liquid pushing controller 42, and a liquid pushing cylinder 43. The liquid pushing cylinder 43 is arranged between the reagent storage tank 41 and the liquid pushing controller 42. A liquid level gauge 411 is provided inside the reagent storage tank 41. Inside the reagent replenishment tank 6, there is a replenishment water pump 62. The bottom of the replenishment water pump 62 is connected to a water suction pipe 63. A replenishment water pipe 621 is connected between the reagent storage tank 41 and the replenishment water pump 62. The liquid level gauge 411 is connected to the replenishment water pump 62. The reagent storage tank 41 is used to store the tracer, and the liquid level gauge 411 is used to detect the liquid level height of the tracer in the reagent storage tank 41. Based on the data transmitted by the liquid level gauge 411, the replenishment water pump 62 is controlled to timely replenish the tracer into the reagent storage tank 41. The replenishment water pump 62 transports the tracer through the water suction pipe 63 and the replenishment water pipe 621. The liquid pushing controller 42 is used to control the liquid pushing activity of the liquid pushing cylinder 43.

[0024] Below the liquid-pushing cylinder 43 is provided with a syringe 44. The bottom of the syringe 44 is connected with an injection head 441. One end of the injection hose 53 far from the perfusion needle 51 is connected with the injection head 441. A connecting water pipe 412 is connected between the reagent storage tank 41 and the syringe 44. A liquid-pushing rod 431 is arranged in the liquid-pushing cylinder 43. One end of the liquid-pushing rod 431 far from the liquid-pushing cylinder 43 is connected with a liquid-pushing piston 432. A connecting air pipe 421 is connected between the liquid-pushing controller 42 and the liquid-pushing cylinder 43. One side of the liquid-pushing controller 42 far from the connecting air pipe 421 is connected with an air inlet pipe 422. The tracer in the reagent storage tank 41 enters the syringe 44 through the connecting water pipe 412, and the syringe 44 injects the tracer into the injection hose 53 through the injection head 411. The liquid-pushing controller 42 is connected with the air storage tank through the air inlet pipe 422, and the liquid-pushing controller 42 can control the telescopic length of the liquid-pushing rod 431 in the liquid-pushing cylinder 43 by intelligently controlling the equivalent of the input gas of the connecting air pipe 421, and the tracer in the syringe 44 is pushed out from the injection head 441 through the extrusion of the liquid-pushing piston 432, so as to control the amount of the liquid-pushing injection of the tracer.

[0025] Working principle: When using an immuno-labeling instrument convenient for adjusting the reagent amount, first, the refrigerated floor cabinet 1 can be opened and closed through the cabinet door 11. Each reagent and the container for labeling cells are placed in the refrigerated floor cabinet 1. The cell container to be detected is placed in the rotary connection disk 2. The tracer can be added into the reagent replenishment tank 6 through the liquid inlet 61. The liquid level gauge 411 detects the liquid level height of the tracer in the reagent storage tank 41, and controls the replenishment water pump 62 to replenish the tracer into the reagent storage tank 41 in time according to the data transmitted by the liquid level gauge 411. The replenishment water pump 62 transports the tracer through the water suction pipe 63 and the replenishment water pipe 621.

[0026] The perfusion device 5 is driven by the perfusion lifting cylinder 7 to move up and down, so that the perfusion needle 51 approaches or moves away from the cell container, and thus the perfusion work can be carried out continuously for multiple times. The perfusion lifting cylinder 7 drives the lifting connecting rod 71 to move up and down, and drives the connecting hose 52 to expand and contract through the connecting collar 711. During the expansion and contraction process of the connecting hose 52, the connecting hose 52 is pulled. At the same time, the tracer in the reagent storage tank 41 enters the syringe 44 through the connecting water pipe 412, and the syringe 44 injects the tracer into the injection hose 53 through the injection head 411. The liquid-pushing controller 42 is connected with the air storage tank through the air inlet pipe 422, and the liquid-pushing controller 42 can control the telescopic length of the liquid-pushing rod 431 in the liquid-pushing cylinder 43 by intelligently controlling the equivalent of the input gas of the connecting air pipe 421, and the tracer in the syringe 44 is pushed out from the injection head 441 through the extrusion of the liquid-pushing piston 432, so as to control the amount of the liquid-pushing injection of the tracer.

Claims

1. An immunolabeling instrument facilitating the adjustment of reagent dosage, comprising a refrigerated bottom cabinet (1), an equipment top box (4) and a perfusion device (5), characterized in that: One side of the refrigerated bottom cabinet (1) is provided with a cabinet door (11). The center position of the top of the refrigerated bottom cabinet (1) is provided with a rotary connection plate (2). And the top of the side of the refrigerated bottom cabinet (1) away from the cabinet door (11) is connected with a connecting side plate (3). The equipment top box (4) is connected to the top of the connecting side plate (3). The side of the connecting side plate (3) away from the rotary connection plate (2) is connected with a reagent replenishing tank (6). And a liquid inlet (61) is arranged at the top of the reagent replenishing tank (6). The perfusion device (5) is connected to the bottom of the equipment top box (4). The perfusion device (5) includes a perfusion needle (51) and a connecting hose (52). And the perfusion needle (51) is connected to the bottom of the connecting hose (52). An injection hose (53) is arranged inside the connecting hose (52). And one end of the injection hose (53) is connected to the perfusion needle (51).

2. The immunolabeling instrument for facilitating the adjustment of the reagent dosage according to claim 1, wherein: One side of the perfusion device (5) is provided with a perfusion lifting cylinder (7). And a lifting connecting rod (71) is connected between the connecting hose (52) and the perfusion lifting cylinder (7). Connection collar rings (711) are arranged at the connection positions of the lifting connecting rod (71) with the connecting hose (52) and the perfusion lifting cylinder (7).

3. An immunolabeling instrument for facilitating the adjustment of reagent dosage according to claim 1, characterized in that: A reagent storage tank (41), a liquid pushing controller (42) and a liquid pushing cylinder (43) are arranged inside the equipment top box (4). And the liquid pushing cylinder (43) is arranged between the reagent storage tank (41) and the liquid pushing controller (42). A liquid level gauge (411) is arranged inside the reagent storage tank (41).

4. An immunolabeling instrument facilitating adjustment of reagent dosage according to claim 3, characterized in that: A replenishing water pump (62) is arranged inside the reagent replenishing tank (6). And a water suction pipe (63) is connected to the bottom of the replenishing water pump (62). A replenishing water pipe (621) is connected between the reagent storage tank (41) and the replenishing water pump (62). And the liquid level gauge (411) is connected to the replenishing water pump (62).

5. The immunolabeling instrument for facilitating the adjustment of reagent dosage according to claim 3, characterized in that: A syringe (44) is arranged below the liquid pushing cylinder (43). An injection head (441) is connected to the bottom of the syringe (44). And the end of the injection hose (53) away from the perfusion needle (51) is connected to the injection head (441). A connecting water pipe (412) is connected between the reagent storage tank (41) and the syringe (44).

6. The immunolabeling instrument for facilitating the adjustment of the reagent dosage according to claim 3, wherein: A liquid pushing rod (431) is arranged inside the liquid pushing cylinder (43). And a liquid pushing piston (432) is connected to the end of the liquid pushing rod (431) away from the liquid pushing cylinder (43). A connecting air pipe (421) is connected between the liquid pushing controller (42) and the liquid pushing cylinder (43). And an air inlet pipe (422) is connected to the side of the liquid pushing controller (42) away from the connecting air pipe (421).

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