Detection optical module and fluorescence detection device

Abstract

The utility model discloses a detection optical module and fluorescence detection device, the detection optical module comprises a light source, a lens, a dichroic mirror and a camera, the light emitted by the light source passes through the lens to form parallel light, the parallel light is filtered by the dichroic mirror to form detection light in a preset wavelength range, and the detection light is sent to the camera. The detection light is reflected by the dichroic mirror to reach a test strip detection area; the camera is arranged on a central axis of a position where a test strip detection area is located; the fluorescence detection device comprises a reagent card bearing module, a detection optical module and a control processing module, wherein the control processing module is connected with the reagent card bearing module and the detection optical module; the reagent card bearing module is used for bearing a reagent card; the embodiment of the utility model can reduce the influence of stray light on the background, improve the uniform distribution of light intensity and improve the imaging quality, and can be widely applied to the technical field of fluorescence detection.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence detection, in particular to a detection optical module and a fluorescence detection device. Background Technique

[0002] Immunochromatography technology is a technology that achieves the purpose of detection through antigen-antibody binding reactions during chromatography. Due to its advantages such as simple operation, rapidity, no need for personnel training, and the requirement of only simple instruments or no instruments at all, this technology has been widely used in many fields such as food detection, drug detection, environmental monitoring, and clinical diagnosis.

[0003] Immunofluorescence technology is based on the principle of antigen-antibody reaction. First, a fluorescent substance is conjugated to a known antigen or antibody, and then this fluorescent antibody (or antigen) is used as a probe to detect the corresponding antigen (or antibody) in serum, body fluids, cells, or tissues. The antigen-antibody complex formed in tissues or cells contains labeled fluorescent substances. The fluorescent substances emit fluorescence when irradiated by external excitation light, and qualitative and quantitative analyses of the antigen or antibody to be detected are carried out through indicators such as fluorescence intensity.

[0004] Existing fluorescence detection devices have the following problems: The spectral range of the light source is relatively wide, which easily causes overexposure of the background and thus affects the interpretation of low fluorescence signal values; the light intensity uniformity on the surface of the reagent card is relatively poor, affecting the detection accuracy; the position of the camera is unreasonable, affecting the imaging quality. Content of the Utility Model

[0005] In view of this, the purpose of the embodiments of the present utility model is to provide a detection optical module and a fluorescence detection device based on the detection optical module, which can reduce the influence of stray light on the background, improve the uniform distribution of light intensity, and improve the imaging quality.

[0006] The present utility model provides a detection optical module, including a light source, a lens, a dichroic mirror, and a camera. The light emitted by the light source forms parallel light through the lens. The parallel light is filtered by the dichroic mirror to form detection light within a preset wavelength range. The detection light is reflected by the dichroic mirror and reaches the test strip detection area; the camera is arranged on the central axis of the position where the test strip detection area is located.

[0007] Optionally, the lens includes a convex lens; the diameter of the lens is larger than the diameter of the light source.

[0008] Optionally, the distance from the optical center of the lens to the center of the dichroic mirror is greater than or equal to the focal length of the lens.

[0009] Optionally, the included angle between the dichroic mirror and the parallel light is 45 degrees, and the included angle between the dichroic mirror and the plane where the test strip detection area is located is 45 degrees.

[0010] Optionally, the linear distance range between the camera and the plane where the test strip detection area is located is 25 mm - 35 mm.

[0011] Optionally, the perpendicular distance range between the center of the dichroic mirror and the plane where the test strip detection area is located is 12 mm - 16 mm.

[0012] The present utility model also provides a fluorescence detection device, which includes the above-mentioned detection optical module, reagent card carrying module and control processing module. The control processing module is connected to the reagent card carrying module and the detection optical module, and the reagent card carrying module is used to carry the reagent card.

[0013] Optionally, the reagent card carrying module includes a guide rail, a slider and a positioning mechanism. The reagent card is moved through the guide rail and the slider, and the center of the test strip detection area of the reagent card is aligned with the camera through the positioning mechanism.

[0014] Optionally, the fluorescence detection device further includes a display module. The display module is connected to the control processing module, and the display module includes a display screen.

[0015] Optionally, the fluorescence detection device further includes a temperature control module and / or an alarm module. The temperature control module is connected to the control processing module, and the alarm module is communicatively connected or electrically connected to the control processing module.

[0016] The detection optical module of the present utility model and the fluorescence detection device based on the detection optical module have the following beneficial effects: The detection optical module includes a light source, a lens, a dichroic mirror and a camera. The light emitted by the light source forms parallel light through the lens, and the parallel light is filtered by the dichroic mirror to form detection light within a preset wavelength range. The detection light is reflected by the dichroic mirror and reaches the test strip detection area. Since the light outside the excitation light wave of the reagent card is filtered out during the process, the influence of stray light is effectively reduced; by adjusting the device structure, the test strip is irradiated with parallel light, the light intensity uniformity on the surface of the test strip is improved, and thus the detection accuracy is improved; by arranging the camera on the central axis of the test strip detection area, a better detection image can be captured and the imaging quality is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic structural diagram of a detection optical module and a fluorescence detection device provided by an embodiment of the present utility model;

[0018] Figure 2 is a schematic diagram of the composition modules of a fluorescence detection device provided by an embodiment of the present utility model;

[0019] Figure 3It is an optical path diagram of a light source passing through a lens provided by an embodiment of the present utility model;

[0020] Figure 4 It is a schematic structural diagram of another fluorescence detection device provided by an embodiment of the present utility model;

[0021] Figure 5 It is a light intensity distribution diagram of the shooting area of a fluorescence detection device and a fluorescence image of a standard reagent card provided by an embodiment of the present utility model; Figure 5 In (a), it represents the light intensity distribution image of the shooting area of the fluorescence detection device in this embodiment, Figure 5 In (b), it represents the optical image of the fluorescence detection device shooting the standard reagent card in this embodiment;

[0022] Figure 6 It is the light intensity distribution diagram of the shooting area and the fluorescence image of the standard reagent card provided by the related device; Figure 6 In (a), it represents the light intensity distribution image of the shooting area of the related fluorescence detection device, Figure 6 In (b), it represents the optical image of the related fluorescence detection device shooting the standard reagent card. Specific embodiments

[0023] The following further elaborates on the present utility model in detail in conjunction with the accompanying drawings and specific embodiments.

[0024] When performing large-scale fluorescence immunoassay detection, an immuno-fluorescence quantitative analyzer is usually used to interpret the fluorescence intensity of the reagent card, and qualitative or quantitative results are obtained by means of a built-in algorithm.

[0025] The fluorescence quantitative analyzer mainly consists of an optical part, a hardware circuit part, and a system software part. The optical part is the core part of the fluorescence quantitative analyzer, which is used to excite fluorescence, while the hardware circuit part and the system software part are used to detect and process the fluorescence signal and control the normal operation of the entire instrument.

[0026] An embodiment of the present utility model provides a detection optical module including a light source, a lens, a dichroic mirror, and a camera. The light emitted by the light source forms parallel light through the lens. When the parallel light passes through the dichroic mirror, it is divided into detection light within a preset wavelength range and stray light outside the preset wavelength range. Among them, the stray light passes through the dichroic mirror and is discarded, while the detection light is reflected by the dichroic mirror and reaches the test strip detection area; the camera is arranged on the central axis of the position where the test strip detection area is located.

[0027] Refer to Figure 1 , the detection optical module includes a light source 1-1, a lens 1-2, a dichroic mirror 1-3, and a camera 1-5. The detection optical module also includes a support component for the optical components. It should be noted that the light source includes but is not limited to an LED light source.

[0028] The light emitted by the LED light source reaches the dichroic mirror through the lens. Selective reflection and light transmission occur at the dichroic mirror. Among them, the light with a wavelength below 365 nm is reflected by the dichroic mirror to the surface of the object to be inspected, and the light with a wavelength above 400 nm is transmitted through the dichroic mirror and discarded. Refer to Figure 3 , when the light passes through the lens, a converging phenomenon occurs, gathering the original scattered light into a relatively parallel light beam and emitting it to the dichroic lens; then, through the beam splitting and reflection of the dichroic mirror, light with a uniform light field intensity distribution and a wavelength below 365 nm is obtained.

[0029] The detection optical module uses a light source to irradiate the surface of the test strip of the reagent card, exciting the fluorescein on the test strip to generate fluorescence, and then obtaining an image of the test strip detection area (in this utility model, the test strip detection area refers to the area including the T line and the C line) through camera shooting. After that, a dedicated data processing module calculates the optical signals corresponding to the T line and the C line (the optical signal can be the area ratio of the wave peaks corresponding to the T line and the C line), substitutes them into the standard curve, and analyzes to obtain the detection result of the sample to be detected.

[0030] Obviously, due to certain differences in the length, width, and the scribing positions of the C line and the T line of different types of test strips, it is difficult for this utility model to strictly define the area of the test strip detection area. However, it should be noted that the test strip detection area described in this utility model at least refers to the positions where the C line and the T line are located, such as the NC membrane of the test strip, and the range of the camera shooting area should at least ensure that it can include the C line and the T line of the test strip. Generally speaking, in order to adapt to different-shaped test strip cartridges, the range of the camera shooting area must be set to be larger than the range of the test strip detection area.

[0031] Optionally, the lens includes a convex lens; the diameter of the lens is greater than the diameter of the light source.

[0032] To obtain the best light focusing effect, the lens is closely attached to the LED lamp bead. For the convenience of installation, the lens is preferably a convex lens, especially a plano-convex lens. In a specific embodiment, the wavelength of the LED light source is above 365 nm, and the thickness of the lens is 5 - 8 mm. The diameter of the lens should be greater than the diameter of the LED lamp bead. For example, if the diameter of the LED lamp bead is 5 mm, the diameter of the lens is 15 mm.

[0033] Optionally, the distance from the optical center of the lens to the center of the dichroic mirror is greater than or equal to the focal length of the lens.

[0034] When the distance from the optical center of the lens to the center of the dichroic mirror is greater than or equal to the focal length of the lens, the point light source will form parallel light with a more uniform light field after passing through the lens. The focal length range of the lens can be selected as 10 - 20 mm. For example, in a specific embodiment, the degree of the convex lens used is 15 degrees, the focal length is 30 mm, and the distance from the optical center of the convex lens to the center of the dichroic mirror is about 30 mm.

[0035] The included angle between the dichroic mirror and the light source is 45 degrees, and the included angle between the dichroic mirror and the test strip detection area is 45 degrees.

[0036] Optionally, the linear distance range between the camera and the test strip detection area is 25 mm - 35 mm.

[0037] In a specific embodiment, the camera is located above the center position of the test strip detection area, and the linear distance from the camera to the test strip detection area is 30 mm. Since in this embodiment, the camera is set on the central axis of the position where the test strip detection area is located, making the test strip detection area located at the center of the captured image. Therefore, compared with the existing solutions, when maintaining the same camera height, this embodiment can capture the best detection image. Additionally, when the test strips of some reagent cards are larger and wider, the distance between the camera and the test strip detection area can be appropriately increased to obtain a larger shooting range.

[0038] Optionally, the vertical distance range between the center of the dichroic mirror and the test strip detection area is 12 mm - 16 mm.

[0039] In a specific embodiment, the vertical distance from the center of the dichroic mirror to the test strip detection area is about 14 mm.

[0040] The embodiment of the present utility model also provides a fluorescence detection device including a detection optical module. The fluorescence detection device further includes a reagent card carrying module and a control and processing module. The control and processing module is connected to the reagent card carrying module and the detection optical module; wherein, the reagent card carrying module is used to carry the reagent card.

[0041] Refer to Figure 1 and Figure 2 , the fluorescence detection device includes a reagent card carrying module 1 - 4, a detection optical module, and a control and processing module. The reagent card carrying module 1 - 4 is used to carry the reagent card. The detection optical module is used to capture and obtain the optical signal (such as an optical image) of the reagent card. The control and processing module then analyzes the optical image obtained by the detection optical module using an algorithm to obtain qualitative or quantitative detection results. The control and processing module is also used to control the other modules. The fluorescence detection device may further include a storage module and a communication module. The storage module is used to store detection information or result information, and the communication module is used for communication between the modules.

[0042] Among them, the detection optical module includes a light source 1-1, a lens 1-2, a dichroic mirror 1-3, and a camera 1-5. The reagent card carrier module 1-4 includes a test strip detection window area, and the reagent card is carried within the reagent card carrier module 1-4. The detection optical module also includes a support component for the optical components. It should be noted that the light source includes but is not limited to an LED light source.

[0043] Optionally, the reagent card carrier module includes a guide rail, a slider, and a positioning mechanism. The reagent card is moved through the guide rail and the slider, and the center of the test strip detection area is aligned with the camera through the positioning mechanism.

[0044] Specifically, the guide rail and the slider are used to assist in pushing the reagent card into or out of the detection device, and the positioning mechanism is used to align the center of the test strip detection area with the camera.

[0045] Optionally, refer to Figure 4 , the fluorescence detection device further includes a display module. The display module is connected to the control processing module, and the display module includes a display screen.

[0046] Specifically, the display module is used to display the relevant information input by the input module or the detection result, and can also display information such as the real-time time and date, GPS positioning, temperature, detection item, detection time, detection result, detection number, etc. transmitted through the communication module and the detection module.

[0047] Optionally, refer to Figure 4 , the fluorescence detection device further includes a temperature control module. The temperature control module is connected to the control processing module.

[0048] Specifically, the temperature control module is used to provide a suitable temperature environment for the detection process.

[0049] Optionally, refer to Figure 4 , the fluorescence detection device further includes an alarm module. The alarm module is connected to the control processing module.

[0050] Specifically, the alarm module is used to provide a warning function.

[0051] Refer to Figure 4 , the fluorescence detection device may further include an input module. The input module is used to obtain input information; the input module can be a keyboard or a touch screen, and is used to input relevant information according to the user's needs. The fluorescence detection device may also include a positioning module for providing positioning information, etc.

[0052] Embodiment 1

[0053] The fluorescence detection device of this embodiment includes a reagent card carrier module, a detection optical module, and a control and processing module. The control and processing module is connected to the reagent card carrier module and the detection optical module. Among them, the reagent card carrier module is used to carry the reagent card. The detection optical module includes a light source, a plano-convex lens, a dichroic mirror, and a camera. The light emitted by the light source forms parallel light through the lens. When the parallel light passes through the dichroic mirror, it is divided into detection light within a preset wavelength range and stray light outside the preset wavelength range. Among them, the stray light passes through the dichroic mirror and is discarded, while the detection light is reflected by the dichroic mirror and reaches the test strip detection area. The camera is arranged on the central axis of the position where the test strip detection area is located.

[0054] The fluorescence detection device further includes a storage module and a communication module. The storage module is used to store detection information or result information, and the communication module is used for communication between modules.

[0055] The reagent card carrier module includes a guide rail, a slider, and a positioning mechanism. The reagent card is moved through the guide rail and the slider, and the center of the test strip detection area is aligned with the camera through the positioning mechanism.

[0056] The fluorescence detection device includes a display module. The display module is connected to the control and processing module. The display module includes a display screen, which is used to display relevant information input by the input module or detection results, and can also display information such as real-time time and date, GPS positioning, temperature, detection item, detection time, detection result, detection number, etc. transmitted through the communication module and the detection module.

[0057] Among them, the camera is located above the central position of the test strip detection area, and the straight-line distance from the camera to the test strip detection area is 30 mm.

[0058] The angle between the dichroic mirror and the test strip plane is 45 degrees, and the vertical distance from the center of the dichroic mirror to the test strip detection area is about 15 mm.

[0059] The angle between the dichroic mirror and the parallel light is 45 degrees.

[0060] The diopter of the convex lens is 15 degrees, the focal length is 30 mm, and the distance from the optical center of the convex lens to the center of the dichroic mirror is about 30 mm.

[0061] Verify the optical detection effect of the fluorescence detection device of this embodiment:

[0062] Before placing the reagent card, first use the fluorescence detection device of this embodiment to take a light intensity distribution image as shown in (a) of Figure 5 Among them, the position where the center of the aperture is located corresponds to the position where the test strip detection area will be placed.

[0063] Insert the pre-prepared standard reagent card into the fluorescence detection device of this embodiment, and take an optical image of the standard reagent card as shown in Figure 5 (b) in the figure. Among them, the red fluorescence light patterns are the C line and the T line from top to bottom.

[0064] Comparative Example 1

[0065] Adopt the same verification method as in Example 1. Before placing the reagent card, first take an image of the light intensity distribution with a related fluorescence detection device as shown in Figure 6 (a) in the figure. Among them, the position where the center of the aperture is located corresponds to the position of the detection area of the test strip to be placed.

[0066] Insert the standard reagent card of the same batch as in Example 1 into the fluorescence detection device of this comparative example, and take an optical image of the standard reagent card as shown in Figure 6 (b) in the figure. Among them, the red fluorescence light patterns are the C line and the T line from top to bottom, and the light gray part between the C line and the T line corresponds to the NC membrane of the test strip.

[0067] Comparing the shooting results of Example 1 and Comparative Example 1, the illumination center of the embodiment of the present utility model basically coincides with the image center. Since the light spot area irradiated on the detection area of the test strip is large and the light intensity is uniform, it can completely and evenly cover the detection area of the test strip, so the best fluorescence image can be taken, and the distinction between the fluorescence brightness of the C line and the T line and the brightness of the NC membrane of the test strip and the image background is very obvious; while in the existing device, the illumination position is significantly offset from the image center, and due to the small light spot and uneven light intensity distribution irradiated on the detection area of the test strip, the distinction between the fluorescence brightness of the C line and the T line and the brightness of the NC membrane of the test strip and the image background in the taken optical image is poor.

[0068] It can be seen that compared with the existing device, in the optical image taken by the fluorescence detection device of this embodiment, the distinction between the NC membrane, C line, T line and background area of the test strip is obvious, which is more conducive to the algorithm to further identify and analyze the image.

[0069] The fluorescence detection device of the present utility model filters out the light outside the excitation light wave of the reagent card during the detection process, so it can effectively reduce the influence of stray light; by adjusting the device structure, it realizes the use of parallel light to irradiate the test strip, improves the uniformity of the light intensity on the surface of the test strip, and further improves the detection accuracy of the device; and by setting the camera on the central axis of the detection area of the test strip, it can take a better detection image and effectively improve the imaging quality of the device.

[0070] Although the longer the optical path of the fluorescence detection device, the larger the uniform range of the fluorescent light spot will be, and the better it can cover the detection window area, the fluorescence intensity within the light spot range will be reduced, which will be detrimental to image recognition. In addition, the handheld instrument is also subject to size and space limitations, which means that the optical path cannot be set too long. For the new detection device after the optimized structure, after comprehensive experimental evaluation, the upper limit of the optical path length of the fluorescence detection device of the utility model is set at 65mm. The optical path length from the light source to the test strip detection area in the existing detection device is about 20 to 25mm. Through the scheme of the embodiment of the utility model, the optical path can be extended to 40 to 65mm, which effectively complies with the two contradictory requirements of handheld portability requiring a smaller size design and uniform light field intensity distribution requiring a larger instrument space, achieving the effect of maintaining the portable characteristics of the detection device and greatly improving the uniformity of light intensity distribution. In addition, the fluorescence detection device provided by the utility model does not need to add filters to the detection optical module, effectively simplifying the number of components and the device structure, and further reducing the dependence of handheld instruments on thickness dimensions.

[0071] The above is a specific description of the preferred implementation of the utility model, but the invention of the utility model is not limited to the described embodiments. Technical personnel familiar with the field can also make various equivalent deformations or substitutions without violating the spirit of the utility model. These equivalent deformations or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A detection optical module, characterized in that, It includes a light source, a lens, a dichroic mirror and a camera. The light emitted by the light source forms parallel light after passing through the lens. The parallel light is filtered by the dichroic mirror to form detection light within a preset wavelength range. The detection light is reflected by the dichroic mirror and reaches the test strip detection area. The camera is arranged on the central axis of the position where the test strip detection area is located.

2. The detection optical module according to claim 1, wherein The lens includes a convex lens. The diameter of the lens is larger than the diameter of the light source.

3. The detection optical module according to claim 1, wherein The distance from the optical center of the lens to the center of the dichroic mirror is greater than or equal to the focal length of the lens.

4. The detection optical module according to claim 1, wherein The angle between the dichroic mirror and the parallel light is 45 degrees, and the angle between the dichroic mirror and the plane where the test strip detection area is located is 45 degrees.

5. The detection optical module according to claim 1, wherein The linear distance range between the camera and the plane where the test strip detection area is located is 25 mm - 35 mm.

6. The detection optical module according to claim 1, characterized in that, The vertical distance range between the center of the dichroic mirror and the plane where the test strip detection area is located is 12 mm - 16 mm.

7. A fluorescence detection device, characterized in that, It includes a reagent card carrying module, a control and processing module, and the detection optical module according to any one of claims 1 - 6. The control and processing module is connected to the reagent card carrying module and the detection optical module. The reagent card carrying module is used to carry a reagent card.

8. The fluorescence detection device according to claim 7, wherein The reagent card carrying module includes a guide rail, a slider and a positioning mechanism. The reagent card is moved through the guide rail and the slider, and the center of the test strip detection area is aligned with the camera through the positioning mechanism.

9. The fluorescence detection device according to claim 7, wherein, The fluorescence detection device further includes a display module. The display module is connected to the control and processing module. The display module includes a display screen.

10. The fluorescence detection device according to claim 7, characterized in that, The fluorescence detection device further includes a temperature control module and / or an alarm module. The temperature control module is connected to the control and processing module. The alarm module is communicatively connected or electrically connected to the control and processing module.

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