Dairy product detection workstation
By designing a dairy product testing workstation and using automation equipment and transfer equipment to transfer vessels and solutions between each equipment, the problem of complex and low efficiency of dairy products in the existing technology is solved, and efficient automated detection is achieved.
Patent Information
- Application Number
- CN202422283138.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-19
- Publication Date
- 2025-07-22
- Estimated Expiration
- 2034-09-19
AI Technical Summary
In the prior art, the detection process of dairy products is complex, the detection efficiency is low, and automation cannot be achieved.
A dairy product testing workstation is designed, including oscillation equipment, centrifugal equipment, reagent buffering equipment, constant temperature equipment, microplate reader and load transfer equipment. Sample processing, mixing, incubation and detection are realized through automated processes. The transfer equipment is used to transfer vessels and solutions between the equipment, and sample components are obtained in combination with a visual detection device.
It realizes the automation of aflatoxin detection for dairy products, improves detection efficiency, saves manpower, and can quickly and accurately judge the content of aflatoxin in dairy products.
Smart Images

Figure CN223139584U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of sample detection, in particular to a dairy product detection workstation. Background Art
[0002] During the production process of dairy products, aflatoxin detection is usually required. In the prior art, aflatoxin is usually manually detected by testers, and the detection process is complex and the detection efficiency is low. Summary of the Utility Model
[0003] The utility model provides a dairy product detection workstation to solve the defects in the prior art that aflatoxin is manually detected, the detection process is complex, and the detection efficiency is low.
[0004] The utility model provides a dairy product detection workstation, including: a shaking device, a centrifugal device, a reagent buffer device, a constant temperature device, an ELISA reader, and a transfer device;
[0005] The shaking device is used for shaking the container filled with dairy products, and the transfer device is used for sampling from the container and transferring the obtained dairy product sample to a centrifuge tube in the centrifugal device;
[0006] The centrifugal device is used for centrifuging the centrifuge tube, and the transfer device is used for sucking away the fat precipitated on the surface layer of the dairy product sample after centrifugation and transferring the defatted dairy product sample to a microplate;
[0007] The reagent buffer device is used for storing standard samples and test reagents for aflatoxin comparison tests, and the transfer device is used for mixing the test reagents with the standard samples and the dairy product samples respectively based on the microplate to obtain a first mixed solution and a second mixed solution; the transfer device is used for transferring the microplate into the constant temperature device;
[0008] The constant temperature device is used for heating and incubating the first mixed solution and the second mixed solution contained in the microplate, and the transfer device is used for transferring the microplate to the ELISA reader;
[0009] The ELISA reader is used for detecting the first mixed solution and the second mixed solution after incubation to obtain the content of aflatoxin in the dairy product.
[0010] According to the dairy product detection workstation of the utility model, it further includes: a visual detection device;
[0011] The transfer device is further used for transferring the dairy product sample to the microplate and transferring the microplate to the constant temperature device;
[0012] The constant temperature device is also used to incubate the dairy product sample, and the visual detection device is used to visually detect the incubated dairy product sample to obtain the content of antibiotics in the dairy product.
[0013] According to the dairy product detection workstation of the present utility model,
[0014] The transfer device includes a first robotic arm, a second robotic arm, and a pipetting device; the first robotic arm is provided with a first pipette; the second robotic arm is provided with a first clamping mechanism; the reagent buffer device includes a first buffer member and a second buffer member; the first buffer member is used to store the standard sample, and the second buffer member is used to store the test reagent;
[0015] The first robotic arm samples from the container through the first pipette, transfers the dairy product sample obtained by sampling into the centrifuge tube, and is used to suck away the fat precipitated on the surface of the dairy product sample after centrifugation, and transfer the dairy product sample after centrifugation and fat suction to the microplate;
[0016] The pipetting device is used to transfer the standard sample to the microplate, and transfer the test reagent stored in the second buffer member to the microplate
[0017] The second robotic arm picks up the microplate through the first clamping mechanism, places the microplate in the constant temperature device, and transfers the microplate from the constant temperature device to the microplate reader.
[0018] According to the dairy product detection workstation of the present utility model, the shaking device includes: a shaking device and a first cap screwing device; the first robotic arm is also provided with a second clamping mechanism;
[0019] The shaking device is used to shake the container containing the dairy product, the first robotic arm transfers the container from the shaking device to the first cap screwing device through the second clamping mechanism, and the first cap screwing device is used to open the cap of the container.
[0020] According to the dairy product detection workstation of the present utility model, the centrifugation device includes: a centrifugation device, a test tube rack, and a second cap screwing device;
[0021] The test tube rack is used to store the centrifuge tube; the second cap screwing device is used to open the cap of the centrifuge tube; the centrifugation device is used to centrifuge the centrifuge tube;
[0022] The first robotic arm transfers the centrifuge tube between the test tube rack, the second cap screwing device, and the centrifugation device through the second clamping mechanism.
[0023] According to the dairy product detection workstation of the present utility model, the constant temperature device includes: an incubation device and a cleaning and drying device;
[0024] The second robotic arm is used to pick up the microplate and transfer the microplate between the incubation device and the cleaning and drying device;
[0025] The incubation device is used to heat and incubate the first mixture and the second mixture in the microplate; the cleaning and drying device is used to clean and dry the microplate.
[0026] According to the dairy product detection workstation of the present utility model, the dairy product sample includes a finished milk sample and a raw milk sample, and the constant temperature device includes a constant temperature heating device;
[0027] The transfer device is also used to transfer the container containing the raw milk to the constant temperature heating device, and the constant temperature heating device is used to heat the raw milk.
[0028] According to the dairy product detection workstation of the present utility model, it further includes a loading and unloading device; the loading and unloading device is arranged at the loading position and the unloading position of the dairy product detection workstation, and the loading and unloading device is used to transfer the container containing the dairy product between the external line and the dairy product detection workstation.
[0029] According to the dairy product detection workstation of the present utility model, the loading and unloading device includes: a third robotic arm, a code scanning device, a loading buffer device, and an unloading buffer device;
[0030] The loading buffer device is arranged at the loading position and is used to buffer the container containing the dairy product to be detected; the code scanning device is used to scan and identify the identification code on the container; the unloading buffer device is used to buffer the container to be recycled;
[0031] The third robotic arm is used to transfer the container between the external line, the loading buffer device, the code scanning device, the oscillation device, the transfer device, and the unloading buffer device.
[0032] According to the dairy product detection workstation of the present utility model, the loading and unloading device further includes a cleaning processing device, and the cleaning processing device is used to clean the container;
[0033] The third robotic arm is also used to transfer the container between the unloading buffer device and the cleaning processing device.
[0034] The dairy product detection workstation of the present utility model shakes and mixes the dairy products to be detected through a shaking device for the sampling of a transfer device. Then, a centrifugal device centrifuges the dairy product samples extracted after shaking to separate out the fat in the dairy product samples. Then, the dairy product samples, standard samples, and test reagents are added to a microplate, so that the dairy product samples and the corresponding test reagents are mixed into a first mixed solution, and the standard samples and the corresponding test reagents are mixed into a second mixed solution. Then, the microplate is transferred to a constant temperature device. After reacting for a period of time under a specific temperature incubation, the microplate is transferred to an ELISA reader for comparison and detection. Finally, the aflatoxin content of the dairy products is obtained to determine whether the aflatoxin content of the dairy products exceeds the standard. At the same time, the transfer device can realize the automatic transfer of vessels among various devices and the automatic transfer of various solutions among various vessels during the detection process, cooperate with various devices to realize the automation of the entire detection process, without manual operation, with high detection efficiency and labor saving. Brief Description of the Drawings
[0035] In order to more clearly illustrate the technical solutions in the present utility model or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.
[0036] Figure 1 is one of the schematic diagrams of the dairy product detection workstation provided by the present utility model.
[0037] Figure 2 is the top view of the dairy product detection workstation provided by the present utility model.
[0038] Figure 3 is Figure 1 the partial schematic diagram at position A in
[0039] Figure 4 is the second schematic diagram of the dairy product detection workstation provided by the present utility model.
[0040] Reference Signs:
[0041] 1. Shaking device; 11. Shaking unit; 12. First cap screwing device;
[0042] 2. Centrifugal device; 21. Centrifugal unit; 22. Test tube rack; 23. Second cap screwing device;
[0043] 3. Reagent buffer device; 31. First buffer member; 32. Second buffer member; 33. Third buffer member; 34. Third cap screwing device;
[0044] 4. Constant temperature equipment; 41. Incubation device; 411. First incubation device; 412. Second incubation device; 42. Cleaning and drying device; 43. Constant temperature heating device;
[0045] 5. Microplate reader;
[0046] 6. Transfer equipment; 61. First robotic arm; 62. Second robotic arm; 63. First pipette rack; 64. Second pipette rack; 65. Transfer conveyor line; 66. Pipetting device;
[0047] 7. Microplate storage rack; 8. Microplate operation table;
[0048] 9. Loading and unloading equipment; 91. Third robotic arm; 92. Scanning device; 93. Loading buffer device; 94. Unloading buffer device; 95. Cleaning and processing equipment;
[0049] 10. Vision inspection device;
[0050] 101. Machine cover; 102. Exhaust unit; 103. Host computer; 104. Alarm lamp. Detailed implementation manners
[0051] To make the objectives, technical solutions and advantages of the present utility model clearer, the technical solutions in the present utility model will be clearly and completely described below with reference to the accompanying drawings in the present utility model. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present utility model without making creative efforts shall fall within the protection scope of the present utility model.
[0052] The following combines Figures 1-4 to describe the dairy product detection workstation of the present utility model.
[0053] As Figure 1As shown in the figure, the utility model provides a dairy product detection workstation, including: a shaking device 1, a centrifugation device 2, a reagent buffer device 3, a constant temperature device 4, an enzyme-labeled immunosorbent assay instrument 5 and a transfer device 6. The shaking device 1 is used for shaking the container filled with dairy products. The transfer device 6 is used for sampling from the container and transferring the obtained dairy product sample to a centrifuge tube in the centrifugation device 2. The centrifugation device 2 is used for centrifuging the centrifuge tube. The transfer device 6 is used for sucking away the fat precipitated on the surface of the dairy product sample after centrifugation and transferring the defatted dairy product sample to a microplate. The reagent buffer device 3 is used for storing standard samples and test reagents for aflatoxin comparison tests. The transfer device 6 is used for mixing the test reagents with the standard samples and dairy product samples respectively based on the microplate to obtain a first mixture and a second mixture. The transfer device 6 is used for transferring the microplate into the constant temperature device 4. The constant temperature device 4 is used for heating and incubating the first mixture and the second mixture contained in the microplate. The transfer device 6 is used for transferring the microplate to the enzyme-labeled immunosorbent assay instrument 5. The enzyme-labeled immunosorbent assay instrument 5 is used for detecting the incubated first mixture and second mixture to obtain the content of aflatoxin in the dairy products.
[0054] It can be understood that when detecting aflatoxin in dairy products, it is usually necessary to prepare standard samples for comparison, sample the dairy products to be detected. After adding the corresponding test reagents to the standard samples and dairy product samples respectively, let the standard samples and dairy product samples react for a period of time at a specific temperature. The standard samples and the corresponding test reagents are mixed to form a first mixture, and the dairy product samples and the corresponding test reagents are mixed to form a second mixture. Then, the first mixture and the second mixture are placed in the enzyme-labeled immunosorbent assay instrument 5 for comparison detection to test the content of aflatoxin in the dairy products.
[0055] It can be understood that the transfer device 6 of this embodiment is simultaneously provided with a transfer device for transporting vessels (such as microplates, test tubes, centrifuge tubes, etc.) and a pipetting device only for transferring liquids, which can not only transport vessels between different devices but also transfer liquids between different vessels.
[0056] The shaking device 1 of this embodiment is used for shaking the container filled with dairy products to shake and mix the dairy products in the container so as to perform subsequent processing on the dairy products.
[0057] After the oscillation is completed, the transfer device 6 extends into the container to aspirate a certain amount (such as 5 ml) of dairy product sample according to the detection requirements, and transfers the dairy product sample to the centrifuge tube of the centrifuge device 2 for centrifuging the dairy product sample. The fat in the centrifuged dairy product sample will precipitate from the surface layer of the dairy product sample. The transfer device 6 can suck away the fat on the surface layer of the dairy product sample for subsequent addition of test reagents to the dairy product sample. It can be understood that the sucked-away fat can be discharged into the waste bucket for centralized treatment. The defatted dairy product sample is then continuously transferred by the transfer device 6 to the microplate.
[0058] The reagent caching device 3 of this embodiment can cache various reagents required during the detection process (such as standard samples for comparison and test reagents). After the dairy product sample is transferred to the microplate, the transfer device 6 can transfer the standard sample to the microplate. It can be understood that the microplate is provided with a microplate array composed of multiple microwells. The transfer device 6 adds the standard sample and the dairy product sample to be detected into different microwells respectively. Then, the transfer device 6 will add the corresponding test reagents into the microwells where the standard sample and the dairy product sample are located respectively, so that the standard sample, the dairy product sample and the corresponding test reagents are fully mixed to form a first mixed solution and a second mixed solution respectively.
[0059] After the addition of the test reagents is completed, the transfer device 6 is used to transfer the microplate into the constant temperature device 4. The constant temperature device 4 can provide a constant temperature environment for heating and incubating the first mixed solution and the second mixed solution contained in the microplate, so that the first mixed solution and the second mixed solution can react at a set temperature. Finally, the microplate containing the reacted first mixed solution and second mixed solution is transferred by the transfer device 6 to the microplate reader 5. The microplate reader 5 can compare the first mixed solution and the second mixed solution in the microplate by colorimetry. Taking the first mixed solution as a reference, the content of aflatoxin in the second mixed solution is measured, so that the content of aflatoxin in the dairy product sample can be obtained, and further determine whether the aflatoxin content in the dairy product exceeds the standard.
[0060] The dairy product detection workstation of the present utility model shakes and mixes the dairy products to be detected through the shaking device 1 for the sampling of the transfer device 6. Then, the centrifugal device 2 centrifuges the dairy product samples extracted after shaking to separate out the fat of the dairy product samples. Then, the dairy product samples, standard samples, and test reagents are added to the microplate, so that the dairy product samples and the corresponding test reagents are mixed into a first mixture, and the standard samples and the corresponding test reagents are mixed into a second mixture. Then, the microplate is transferred to the constant temperature device 4. After reacting for a period of time under specific temperature incubation, the microplate is transferred to the microplate reader 5 for comparison detection. Finally, the aflatoxin content of the dairy products is obtained to determine whether the aflatoxin content of the dairy products exceeds the standard. At the same time, the transfer device 6 can realize the automatic transfer of utensils among various devices during the detection process and the automatic transfer of various solutions among various utensils, cooperate with various devices to realize the automation of the entire detection process, without manual operation, with high detection efficiency and labor saving.
[0061] In some embodiments, the standard sample is a standard solution with a determined concentration of aflatoxin M1 (AFM1). The test reagents include aflatoxin M1 antibody-enzyme conjugate solution, TMB substrate solution (3,3,5,5-Tetramethylbenzidine solution liquid membrane substrate), termination solution, etc. Among them, the aflatoxin M1 antibody-enzyme conjugate solution contains aflatoxin M1 antibody and an enzyme label that can be connected to the antibody, and is used to be added to the standard samples and dairy product samples. Aflatoxin M1 in the standard samples and dairy product samples can compete with the enzyme label for the aflatoxin M1 antibody. The enzyme label bound to the antibody can be adsorbed in the micropores of the microplate, and the enzyme label that does not bind to the antibody in the micropores can be washed away. The TMB substrate solution and the termination solution are used to react with the enzyme label bound to the antibody to generate a chromogenic substance, so as to form a chromogenic first mixture and second mixture, so that the microplate reader 5 can compare and detect the first mixture and the second mixture, and finally obtain the content of aflatoxin M1 in the dairy product samples.
[0062] In some embodiments, the dairy product detection workstation further includes: a vision detection device 10. The transfer device 6 is also used to transfer the dairy product samples to the microplate and transfer the microplate to the constant temperature device 4. The constant temperature device 4 is also used to incubate the dairy product samples, and the vision detection device 10 is used to perform vision detection on the dairy product samples after incubation to obtain the antibiotic content in the dairy products.
[0063] In this embodiment, a vision detection device 10 is provided in the dairy product detection workstation. The vision detection device 10 is used to perform vision detection on the dairy product samples after constant temperature incubation. Through vision detection, the content of antibiotics in the dairy product samples can be determined, so as to obtain the content of antibiotics in the detected dairy products, in order to judge whether the content of antibiotics in the dairy products is within the qualified range.
[0064] Specifically, in some embodiments, as Figure 1 and Figure 2 shown, the dairy product detection workstation is also provided with a microplate storage rack 7 and a microplate operation table 8. The microplate storage rack 7 is used to store microplates. The transfer device 6 is used to transfer the microplates on the microplate storage rack 7 and place them on the microplate operation table 8. The microplate operation table 8 can be used to place microplates, so that the transfer device 6 can add various samples and reagents into the microplates. The microplate operation table 8 can also be used to seal and shake the microplates.
[0065] In some embodiments, as Figure 1 , Figure 2 and Figure 3 shown, the transfer device 6 includes a first robotic arm 61, a second robotic arm 62 and a pipetting device 66. The first robotic arm 61 is provided with a first pipette. The second robotic arm 62 is provided with a first clamping mechanism; the reagent buffer device 3 includes a first buffer member 31 and a second buffer member 32. The first buffer member 31 is used to store standard samples, and the second buffer member 32 is used to store test reagents. The first robotic arm 61 samples from the container through the first pipette, transfers the obtained dairy product samples to the centrifuge tube, and is used to suck away the fat precipitated on the surface of the dairy product samples after centrifugation, and transfer the dairy product samples after centrifugation and fat suction to the microplate. The pipetting device 66 is used to transfer the standard samples to the microplate, and transfer the test reagents stored in the second buffer member 32 to the microplate. The second robotic arm 62 picks up the microplate through the first clamping mechanism, places the microplate in the constant temperature device 4, and transfers the microplate from the constant temperature device 4 to the microplate reader 5.
[0066] In this embodiment, the first robotic arm 61 is provided with a first pipette, which is mainly used to transfer liquids among the container, the centrifuge tube, and the microplate. Specifically, the first robotic arm 61 can sample the dairy products in the container, transfer the obtained dairy product samples to the centrifuge tube, so that the centrifugation device 2 can centrifuge the dairy product samples in the centrifuge tube. After centrifugation, the fat of the dairy product samples precipitates on the surface. The first robotic arm 61 can suck away the fat through the first pipette and transfer the dairy product samples after centrifugation and fat suction to the microplate.
[0067] The pipetting device 66 is mainly used for transferring two solutions, namely the standard sample and the test reagent, into the microplate. Specifically, the pipetting device 66 can aspirate the standard sample and add the aspirated solution into the microplate. At the same time, the second buffer member 32 is arranged within the working range of the pipetting device 66, enabling the pipetting device 66 to transfer various test reagents cached in the second buffer member 32 into the microplate when the microplate already contains the standard sample and the dairy product sample, so as to form the first mixed solution and the second mixed solution.
[0068] In the second robotic arm 62 of this embodiment, a first clamping mechanism is arranged. The first clamping mechanism is used to move the microplate, so as to transfer the microplate containing the first mixed solution and the second mixed solution into the constant temperature device 4 for constant temperature incubation, and transfer the microplate after incubation from the constant temperature device 4 to the microplate reader 5 for monitoring.
[0069] In some embodiments, the first robotic arm 61 is also equipped with a first pipette rack 63. The first pipette rack 63 is used to store the pipette tips adapted to the first pipette. After completing a single liquid transfer, the first robotic arm 61 can move the first pipette to the first pipette rack 63 to replace the pipette tip. The first pipette can drive the pipette tip to aspirate the solution for liquid transfer.
[0070] It can be understood that in some embodiments, the pipetting device 66 can be directly integrated on the second robotic arm 62, enabling the second robotic arm 62 to move the pipetting device 66 and transfer the two solutions of the standard sample and the test reagent into the microplate through the pipetting device 66.
[0071] Optionally, the pipetting device 66 can also be an independent device, such as Figure 3 As shown, the pipetting device 66 includes: a multi-axis adjustment component and a second pipette arranged on the multi-axis adjustment component. The multi-axis adjustment component can adjust the position of the second pipette in the horizontal and vertical directions, so as to aspirate the standard sample or the test reagent through the second pipette and transfer it into the microplate.
[0072] The pipetting device 66 is also equipped with a second pipette rack 64. The second pipette rack 64 is used to store the pipette tips adapted to the second pipette. After completing a single liquid transfer, the pipetting device 66 can control the second pipette to separate and discard the pipette tip, and move to the second pipette rack 64 to connect a new pipette tip. The second pipette can drive the pipette tip to aspirate the solution for liquid transfer.
[0073] In some embodiments, such as Figure 1 and Figure 2As shown in the figure, the shaking device 1 includes a shaking mechanism 11 and a first capping device 12. The first robotic arm 61 is also provided with a second clamping mechanism. The shaking mechanism 11 is used to shake the container filled with dairy products. The first robotic arm 61 transfers the container from the shaking mechanism 11 to the first capping device 12 through the second clamping mechanism, and the first capping device 12 is used to open the cap of the container.
[0074] In this embodiment, by providing the second clamping mechanism on the first robotic arm 61, the first robotic arm 61 can clamp the container and transfer it. Specifically, after the shaking mechanism 11 finishes shaking the container, the first robotic arm 61 can transfer the container to the first capping device 12, and the first capping device 12 opens the cap of the container so that the first pipetting gun of the first robotic arm 61 can extend into the container to sample the dairy products.
[0075] In some embodiments, as Figure 1 and Figure 2 shown in the figure, the centrifugation device 2 includes a centrifugation mechanism 21, a test tube rack 22, and a second capping device 23. The test tube rack 22 is used to store centrifuge tubes. The second capping device 23 is used to open the caps of the centrifuge tubes. The centrifugation mechanism 21 is used to centrifuge the centrifuge tubes. The first robotic arm 61 transfers the centrifuge tubes among the test tube rack 22, the second capping device 23, and the centrifugation mechanism 21 through the second clamping mechanism.
[0076] In this embodiment, the first robotic arm 61 can clamp the centrifuge tubes stored in the test tube rack 22 through the second clamping mechanism and transfer the centrifuge tubes to the second capping device 23. The second capping device 23 can open the caps of the centrifuge tubes so as to add the dairy product samples into the centrifuge tubes. The first robotic arm 61 can transfer the centrifuge tubes from the second capping device 23 to the centrifugation mechanism 21, and the centrifugation mechanism 21 can centrifuge the centrifuge tubes filled with the dairy product samples.
[0077] In some embodiments, as Figure 1 and Figure 2 shown in the figure, the thermostatic device 4 includes an incubation mechanism 41 and a cleaning and drying device 42. The second robotic arm 62 is used to pick up the microplate and transfer the microplate between the incubation mechanism 41 and the cleaning and drying device 42. The incubation mechanism 41 is used to heat and incubate the first and second mixtures in the microplate. The cleaning and drying device 42 is used to clean and dry the microplate.
[0078] In this embodiment, the second robotic arm 62 can place the microplate into the incubation device 41 through the first clamping mechanism. The incubation device 41 can provide a constant temperature environment maintained at a set temperature to heat and incubate the mixture on the microplate, enabling the mixture on the microplate to react at the set temperature for subsequent comparison tests. The cleaning and drying device 42 can be used to clean and dry the microplate after the incubation process to remove the enzyme-labeled substances that have not bound to the antibody in the micropores of the microplate.
[0079] It can be understood that during the detection process of aflatoxin M1, test reagents usually need to be added multiple times, and multiple incubations and microplate washings are required. Therefore, the second robotic arm 62 needs to transfer the microplate back and forth between the incubation device 41 and the cleaning and drying device 42.
[0080] It can be understood that the cleaning and drying device 42 includes a microplate washing mechanism, a drying mechanism, and a waste disposal mechanism. The microplate washing mechanism is used to add a cleaning solution into the microplate for cleaning, the drying mechanism is used to dry the microplate by patting the microplate and blowing air onto the microplate, and the waste disposal mechanism is used to cache the discarded microplates after the detection is completed.
[0081] Optionally, in some embodiments, as Figure 1 and Figure 2 shown, the constant temperature device 4 can include multiple incubation devices 41, and the multiple incubation devices 41 are configured with different incubation temperatures to meet different incubation requirements. Specifically, the incubation device 41 includes a first incubation device 411 and a second incubation device 412. The first incubation device 411 is configured with the incubation temperature required during the aflatoxin detection process, and the second incubation device 412 is configured with the incubation temperature required during the antibiotic detection process.
[0082] In some embodiments, as Figure 1 and Figure 2 shown, the dairy product samples include finished milk samples and raw milk samples. The constant temperature device 4 includes a constant temperature heating device 43, and the transfer device 6 is also used to transfer the container filled with raw milk to the constant temperature heating device 43, and the constant temperature heating device 43 is used to heat the raw milk.
[0083] In this embodiment, it can be understood that raw milk cannot be directly used for detection. The transfer device 6 in this embodiment can first transfer the raw milk sample to the constant temperature heating device 43 for heating for subsequent corresponding detections.
[0084] Specifically, in some embodiments, the constant temperature heating device 43 can be a water bath.
[0085] In some embodiments, as Figure 1 and Figure 2As shown in the figure, the dairy product detection workstation further includes a loading and unloading device 9. The loading and unloading device 9 is arranged at the loading position and the unloading position of the dairy product detection workstation, and is used to transfer the container filled with dairy products between the external line and the antibiotic detection workstation.
[0086] In this embodiment, by arranging the loading and unloading device 9 on the dairy product detection workstation, the loading and unloading device 9 is used to receive the container filled with dairy products transported by the external line and unload the container of the dairy product after the detection is completed, so as to realize the automatic loading and unloading of the container filled with dairy products.
[0087] Specifically, in some embodiments, as Figure 1 and Figure 2 shown, the loading and unloading device 9 includes a third robotic arm 91, a code scanning device 92, a loading buffer device 93 and an unloading buffer device 94. The loading buffer device 93 is arranged at the loading position and is used to buffer the container filled with the dairy product to be detected. The code scanning device 92 is used to scan and identify the identification code on the container. The unloading buffer device 94 is used to buffer the container to be recycled. The third robotic arm 91 is used to transfer the container between the external line, the loading buffer device 93, the code scanning device 92, the oscillation device 1, the transfer device 6 and the unloading buffer device 94.
[0088] In this embodiment, the third robotic arm 91 can pick up the container filled with the dairy product to be detected on the external line and place the container on the loading buffer device 93 for buffering. At the start of the detection, the third robotic arm 91 can transfer the container in the loading buffer device 93 to the code scanning device 92 for the code scanning device 92 to identify. The code scanning device 92 can determine the detection items and detection process of the dairy product in the container according to the identification code on the container, so that the dairy product detection workstation can monitor in the subsequent detection process. After the transfer device 6 completes the sampling of the dairy product in the container, the third robotic arm 91 immediately transfers the container to the unloading buffer device 94 for subsequent recycling processing.
[0089] In some embodiments, the loading and unloading device 9 further includes a cleaning and processing device 95, and the cleaning and processing device 95 is used to clean the container. The third robotic arm 91 is also used to transfer the container between the unloading buffer device 94 and the cleaning and processing device 95.
[0090] It is understandable that when detecting the aflatoxin content of dairy products, after the first robotic arm 61 completes sampling the container filled with dairy products, there is still dairy product residue in the container. The container can first be transferred by the third robotic arm 91 to the blanking buffer device 94 for temporary storage. If the subsequent test results show that the aflatoxin content of the dairy product in the container is unqualified, the container can be taken away by the tester for further testing and analysis of the dairy product in the container. If the subsequent test results show that the aflatoxin content of the dairy product in the container is qualified, the third robotic arm 91 can transfer the container to the cleaning and processing device 95 to clean the container. After cleaning, the third robotic arm 91 places the container on the blanking buffer device 94, waiting to be recycled or returned to the external line.
[0091] In a specific embodiment, as Figures 1 to 3 shown, the transfer device 6 includes a first robotic arm 61, a second robotic arm 62, a pipetting device 66, a first gun rack 63, a second gun rack 64, and a transfer conveyor line 65. The loading and unloading device 9 is arranged at one end of the transfer conveyor line 65. The first robotic arm 61 and the pipetting device 66 are located beside the transfer conveyor line 65. The second robotic arm 62 is located at the other end of the transfer conveyor line 65. The pipetting device 66 is located between the first robotic arm 61 and the second robotic arm 62. The transfer conveyor line 65 is used to transport the container between the first robotic arm 61, the second robotic arm 62, and the third robotic arm 91.
[0092] The loading and unloading device 9 includes a third robotic arm 91, a code scanning device 92, a loading buffer device 93, and a blanking buffer device 94. The third robotic arm 91 is located at one end of the transfer conveyor line 65. The loading buffer device 93 and the blanking buffer device 94 are respectively located on both sides of the third robotic arm 91 along the width direction of the transfer conveyor line 65. A cleaning and processing device 95 is also arranged within the working range of the third robotic arm 91.
[0093] A centrifugal device 21, a second cap screwing device 23, a test tube rack 22, a first gun rack 63, a shaking device 11, and a first cap screwing device 12 are arranged within the working range of the first robotic arm 61.
[0094] Among them, the shaking device 11 is arranged between the first robotic arm 61 and the third robotic arm 91 and is within the working ranges of both at the same time. The microplate operation table 8 is arranged between the first robotic arm 61 and the second robotic arm 62 and is within the working ranges of both at the same time.
[0095] Optionally, a temporary storage rack is also arranged within the overlapping working range of the first robotic arm 61 and the third robotic arm 91. The third robotic arm 91 can place the container filled with dairy products after shaking and with the lid opened on the temporary storage rack so that the first robotic arm 61 can sample the dairy product in the container.
[0096] Within the working range of the pipetting device 66, a second buffer 32, a second pipette rack 64, and a microplate operation table 8 are provided.
[0097] Within the working range of the second robotic arm 62, a first incubation device 411, a second incubation device 412, a cleaning and drying device 42, a constant temperature heating device 43, an ELISA reader 5, a microplate storage rack 7, a first buffer 31, a third buffer 33, a third capping device 34, a microplate operation table 8, and a vision detection device 10 are provided. It can be understood that the microplate operation table 8 is simultaneously within the working ranges of the pipetting device 66 and the second robotic arm 62.
[0098] It can be understood that when detecting the antibiotic content of dairy products, when the dairy product is raw milk, the dairy product needs to be heated at a constant temperature first; in addition, when detecting the antibiotic content of dairy products, it is not necessary to centrifuge the dairy product sample. The first robotic arm 61 can place the container containing the dairy product that has been shaken in the shaking device 11 on the transfer conveyor line 65, and the transfer conveyor line 65 transports the container to the second robotic arm 62. The second robotic arm 62 transfers the container to the third buffer 33. The third buffer 33 is used to buffer the containers of dairy products that need to be detected for antibiotics. When antibiotic detection is required and the dairy product is raw milk, the second robotic arm 62 can transfer the container containing the dairy product to the constant temperature heating device 43 for preheating and then cooling, and then transfer the container containing the heated and cooled dairy product to the third capping device 34 for uncapping in order to perform sampling detection; when the dairy product is finished milk, the second robotic arm 62 can directly transfer the container containing the dairy product to the third capping device 34 for uncapping in order to perform sampling detection.
[0099] The container still containing some dairy products after sampling can be temporarily stored in the third buffer 33. If the subsequent test results show that the antibiotic content of the dairy product in the container is unqualified, the container can be taken away by the tester for further testing and analysis of the dairy product in the container; if the subsequent test results show that the antibiotic content of the dairy product in the container is qualified, the second robotic arm 62 can transfer the container to the transfer conveyor line 65, and the transfer conveyor line 65 transfers it to the third robotic arm 91. The third robotic arm 91 picks up the container and places it in the cleaning processing equipment 95 to clean the container. After cleaning, the third robotic arm 91 places the container on the unloading buffer device 94 and waits for recycling or returning to the external line.
[0100] In some embodiments, such as Figure 4As shown, the dairy product detection workstation further includes a hood 101. The hood 101 is buckled on the tops of the shaking device 1, the centrifugal device 2, the reagent buffer device 3, the constant temperature device 4, the ELISA reader 5 and the transfer device 6 to ensure a sterile environment inside the hood 101. A cabinet door is formed on the side of the hood 101, which facilitates the adjustment of various devices inside the hood 101. An opening is provided at a position corresponding to the shaking device 1 in the hood 101, and the opening is provided to facilitate cooperation with an AGV or an external conveyor line for loading and unloading.
[0101] Specifically, the cabinet door can be a glass cabinet door to better observe the inside of the hood 101.
[0102] Further, in some embodiments, the dairy product detection workstation further includes an exhaust unit 102. The exhaust unit 102 is arranged on the top of the hood 101. The exhaust unit 140 can discharge the turbid air inside the hood 130 based on a power system such as a motor to improve the detection environment.
[0103] Further, in some embodiments, the dairy product detection workstation further includes a host computer 103 and an alarm lamp 104. The host computer 103 is arranged outside the hood 101. The host computer 103 is communicatively connected to the shaking device 1, the centrifugal device 2, the reagent buffer device 3, the constant temperature device 4, the ELISA reader 5 and the transfer device 6. The host computer 103 is used to control various devices inside the hood 101. The alarm lamp 104 is arranged on the top of the hood 101. The alarm lamp 104 is communicatively connected to the host computer 103. The alarm lamp 104 is used to indicate the working state of the dairy product detection workstation through lights of different colors to prompt the staff to perform maintenance as soon as possible when the dairy product detection workstation fails.
[0104] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A dairy product detection workstation, characterized in that, Including: A shaking device, a centrifugal device, a reagent caching device, a constant temperature device, an ELISA reader, and a transfer device; The shaking device is used to shake the container filled with dairy products, and the transfer device is used to take samples from the container and transfer the dairy product samples obtained by sampling into the centrifuge tubes in the centrifugal device; The centrifugal device is used to perform centrifugation on the centrifuge tubes, and the transfer device is used to suck away the fat precipitated on the surface of the dairy product samples after centrifugation, and transfer the defatted dairy product samples to the microplates; The reagent caching device is used to store the standard samples and test reagents for aflatoxin comparison tests, and the transfer device is used to mix the test reagents with the standard samples and the dairy product samples respectively based on the microplates to obtain a first mixed solution and a second mixed solution; the transfer device is used to transfer the microplates into the constant temperature device; The constant temperature device is used to heat and incubate the first mixed solution and the second mixed solution contained in the microplates, and the transfer device is used to transfer the microplates to the ELISA reader; The ELISA reader is used to detect the first mixed solution and the second mixed solution after incubation to obtain the content of aflatoxin in the dairy products.
2. The dairy product detection workstation according to claim 1, characterized in that, It further includes: A visual detection device; The transfer device is also used to transfer the dairy product samples to the microplates and transfer the microplates to the constant temperature device; The constant temperature device is also used to incubate the dairy product samples, and the visual detection device is used to visually detect the dairy product samples after incubation to obtain the content of antibiotics in the dairy products.
3. The dairy product detection workstation according to claim 1, wherein The transfer device includes a first robotic arm, a second robotic arm, and a pipetting device; the first robotic arm is provided with a first pipette; the second robotic arm is provided with a first clamping mechanism; the reagent caching device includes a first caching member and a second caching member; the first caching member is used to store the standard samples, and the second caching member is used to store the test reagents; The first robotic arm takes samples from the container through the first pipette, transfers the dairy product samples obtained by sampling into the centrifuge tubes, and is used to suck away the fat precipitated on the surface of the dairy product samples after centrifugation, and transfer the dairy product samples after centrifugation and fat suction to the microplates; The pipetting device is used to transfer the standard samples to the microplates and transfer the test reagents stored in the second caching member to the microplates; The second robotic arm picks up the microplates through the first clamping mechanism, places the microplates in the constant temperature device, and transfers the microplates from the constant temperature device to the ELISA reader.
4. The dairy product detection workstation according to claim 3, wherein The shaking device includes: a shaking device and a first cap screwing device; the first robotic arm is also provided with a second clamping mechanism; The oscillation device is used to oscillate the container filled with dairy products. The first robotic arm transfers the container from the oscillation device to the first capping device through the second clamping mechanism, and the first capping device is used to open the cap of the container.
5. The dairy product detection workstation according to claim 4, wherein, The centrifugation equipment includes: a centrifugation device, a test tube rack, and a second capping device; The test tube rack is used to store the centrifuge tubes; the second capping device is used to open the caps of the centrifuge tubes; the centrifugation device is used to centrifuge the centrifuge tubes; The first robotic arm transfers the centrifuge tubes among the test tube rack, the second capping device, and the centrifugation device through the second clamping mechanism.
6. The dairy product detection workstation according to claim 3, characterized in that The temperature control equipment includes: an incubation device and a cleaning and drying device; The second robotic arm is used to pick up the microplate and transfer the microplate between the incubation device and the cleaning and drying device; The incubation device is used to heat and incubate the first mixed solution and the second mixed solution in the microplate; the cleaning and drying device is used to clean and dry the microplate.
7. The dairy product detection workstation according to claim 1, wherein the dairy product samples include finished milk samples and raw milk samples, characterized in that, The temperature control equipment includes a constant temperature heating device; The transfer equipment is also used to transfer the container filled with raw milk to the constant temperature heating device, and the constant temperature heating device is used to heat the raw milk.
8. The dairy product detection workstation according to claim 1, characterized in that, It also includes a loading and unloading equipment; the loading and unloading equipment is arranged at the loading position and the unloading position of the dairy product detection workstation, and the loading and unloading equipment is used to transfer the container filled with dairy products between the external line and the dairy product detection workstation.
9. The dairy product detection workstation according to claim 8, wherein, The loading and unloading equipment includes: a third robotic arm, a code scanning device, a loading buffer device, and an unloading buffer device; The loading buffer device is arranged at the loading position and is used to buffer the container filled with the dairy product to be detected; the code scanning device is used to scan and identify the identification code on the container; the unloading buffer device is used to buffer the container to be recycled; The third robotic arm is used to transfer the container among the external line, the loading buffer device, the code scanning device, the oscillation equipment, the transfer equipment, and the unloading buffer device.
10. The dairy product detection workstation according to claim 9, characterized in that, The loading and unloading equipment also includes a cleaning treatment equipment, and the cleaning treatment equipment is used to clean the container; The third robotic arm is also used to transfer the container between the unloading buffer device and the cleaning treatment equipment.
11. The dairy product detection workstation according to claim 1, characterized in that, It also includes a hood and an exhaust unit; The hood is buckled on the tops of the oscillation equipment, the centrifugation equipment, the reagent buffer equipment, the temperature control equipment, the ELISA analyzer, and the transfer equipment. A cabinet door is formed on the side surface of the hood, and an opening is arranged at the position corresponding to the oscillation equipment in the hood; The exhaust unit is arranged on the top of the hood.
Citation Information
Cited By
Drug microorganism detection system and detection method
CN120737950A
