Device for quickly cleaning brain slices or spinal cord slices for multiple times

Abstract

The utility model discloses a device for quickly cleaning brain slices or spinal cord slices for multiple times. The device comprises a light-proof top cover, a pore plate, a base, a shaking table and a peristaltic pump, the pore plate is arranged in the base, the light-proof top cover covers the pore plate, a plurality of through holes are formed in the pore plate, and the filter screens are arranged at the bottoms of the through holes; a water inlet hole and a water outlet hole are formed in the two opposite sides of the base and connected with the peristaltic pump, and a base clamping groove used for being connected with the base is formed in the shaking table. And liquid circulation can be carried out on the communicating six-hole plate through the peristaltic pump so as to clean the brain slices. The peristaltic pump can control the water inlet and outlet speed, and the cleaning mode is water storage cleaning or circulating cleaning. The device provided by the utility model can automatically clean a large number of brain or spinal cord tissue slices for multiple times, thereby saving a large amount of research time.

Description

Technical Field

[0001] The utility model belongs to the technical field of neurobiological experiments, and particularly relates to a device for quickly washing brain slices or spinal cord slices multiple times. Background Art

[0002] The frozen sections of brain tissue and spinal cord tissue are important specimens often used in neuroscience research. Due to their relatively thin tissue thickness and clear and stable spatial characteristics, they are often used for the detection of some important markers and quantitative analysis, etc. When detecting relevant markers, it is necessary to combine them with corresponding antibodies for color reaction. When washing the slices of the brain or spinal cord, it is necessary to change the liquid and wash them regularly and repeatedly, which consumes a lot of time and energy of front-line scientific research workers. In this process, it is necessary to transfer and wash the specimens repeatedly. Currently, the commonly used method is to place the specimens in a six-well plate and manually fish out the specimens repeatedly, change the liquid and wash them. The above method of washing specimens not only consumes a lot of time and energy, but also because the frozen specimens are relatively fragile, it is very easy to be damaged when manually transferring the specimens. When washing specimens in multiple groups, it is very easy to be confused when fishing out the specimens to change the liquid, which seriously affects the experiment. Content of the Utility Model

[0003] To solve the above problems, the utility model provides a device for quickly washing brain slices or spinal cord slices multiple times, which saves time and effort in washing specimens, does not require manual washing, and does not require repeated manual interference with specimens, reducing the breakage rate.

[0004] To achieve the above purpose, the technical scheme adopted by the utility model is: a device for quickly washing brain slices or spinal cord slices multiple times, including a light-shielding top cover, a well plate, a base, a shaker, and a peristaltic pump; the well plate is arranged in the base, the light-shielding top cover covers the well plate, a plurality of through holes are opened on the well plate, and a filter screen is arranged at the bottom of the through holes; water inlet holes and water outlet holes are opened on opposite sides of the base, the water inlet holes and the water outlet holes are connected to the peristaltic pump, and a base slot for connecting the base is arranged on the shaker.

[0005] Further, the base is embedded in the base slot, through holes are opened at positions corresponding to the water inlet hole and the water outlet hole of the base in the base slot, and the peristaltic pump is connected to the water inlet hole and the water outlet hole of the base through a hose passing through the through holes.

[0006] Further, a control panel and a switch are arranged on the shaker.

[0007] Further, the peristaltic pump is also connected to a container for holding the liquid for washing or collecting waste liquid.

[0008] Further, a positioning pin is arranged between the well plate and the base.

[0009] Furthermore, a connecting ring is provided at the upper end of the base, the orifice plate is embedded inside the connecting ring, and the light-shielding top cover is snap-fitted outside the connecting ring.

[0010] Furthermore, a carrying ring is provided on the orifice plate.

[0011] Furthermore, a sealing ring is provided at the connection between the orifice plate and the base.

[0012] Compared with the prior art, the present utility model has at least the following beneficial effects:

[0013] Cleaning the specimen saves time and effort, without manual cleaning, and there is no need to repeatedly interfere with the specimen manually, reducing the breakage rate. Moreover, the water inlet and outlet speeds of the peristaltic pump can be set according to the different specimens and specific experimental requirements to adjust the water inlet and outlet speeds, so as to ensure that the specimen is fully cleaned on the premise of minimizing damage to the specimen. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the external structure of the present utility model.

[0015] In the drawings, 1 - light-shielding top cover, 2 - orifice plate, 3 - base, 4 - shaker. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0016] The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the drawings in the embodiments of the present utility model. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0017] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "one side", "one end", "one side" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, unless otherwise specified, the meaning of "a plurality" is two or more.

[0018] Various structural schematic diagrams according to the disclosed embodiments of the present utility model are shown in the accompanying drawings. These figures are not drawn to scale, where for the purpose of clear expression, some details are enlarged and some details may be omitted. The shapes of various regions and layers shown in the figures, as well as their relative sizes and positional relationships, are merely exemplary. In practice, there may be deviations due to manufacturing tolerances or technical limitations, and those skilled in the art can additionally design regions / layers with different shapes, sizes, and relative positions according to actual requirements.

[0019] Referring Figure 1 , a device for quickly cleaning brain slices or spinal cord slices multiple times provided by the present application includes a light-shielding top cover 1, a well plate 2, a base 3, a shaker 4, and a peristaltic pump 5; the well plate 2 is arranged in the base 3, the light-shielding top cover 1 covers the well plate 2, multiple through holes are opened on the well plate 2, and a filter screen 21 is arranged at the bottom of the through holes; water inlet holes and water outlet holes are opened on opposite sides of the base 3, the water inlet holes and the water outlet holes are connected to the peristaltic pump 5, and a base slot 41 for connecting the base 3 is arranged on the shaker 4; the peristaltic pump 5 is further connected to a container for holding the liquid for cleaning or collecting waste liquid, and the liquid for cleaning or the waste liquid is collected.

[0020] The shaker 4 and the peristaltic pump 5 are commercially available products.

[0021] Optionally, a positioning pin is arranged between the well plate 2 and the base 3 to facilitate accurate connection; the positioning pin can ensure the accurate position of the well plate 2 on the base 3 and prevent deviation or misalignment during the assembly process; through the fixing effect of the positioning pin, the connection between the well plate 2 and the base 3 is more stable, can withstand certain external forces and vibrations, and is not easy to loosen or fall off. The positioning pin makes the assembly process simpler and faster, reducing the time for adjustment and alignment; a straight-column type positioning pin or a tapered positioning pin can be used; corresponding positioning holes are designed on the well plate 2 and the base 3 to ensure that the positioning pin can be smoothly inserted and play a positioning role. The size and shape of the positioning holes should match the positioning pin to ensure good fitting accuracy.

[0022] As a preferred embodiment, a connecting ring is provided at the upper end of the base 3. The orifice plate 2 is embedded inside the connecting ring, and the light-shielding top cover 1 is snap-fitted outside the connecting ring; this makes the connection between the base, the orifice plate, and the top cover firm and stable, and it is not likely to fall off during shaking. A handle ring is provided on the orifice plate 2 to facilitate various operations on the orifice plate 2. A sealing ring is provided at the connection between the orifice plate 2 and the base 3 to prevent the cleaning liquid from splashing out during shaking. The base 3 serves as the foundation of the entire structure, and a connecting ring is designed at its upper end. This connecting ring not only provides interfaces for connecting with the orifice plate 2 and the light-shielding top cover 1, but also ensures the strength and stability of the structure through its shape and material. The inner diameter and shape of the connecting ring need to be precisely designed to fit closely with the orifice plate. The orifice plate 2 is designed to be embedded inside the connecting ring. The embedded design not only simplifies the assembly process but also enhances the connection between the orifice plate and the base through physical contact and possible clamping mechanisms; the holes on the orifice plate can be designed for experimental or operational needs, such as liquid circulation. The light-shielding top cover 1 is designed to be snap-fitted outside the connecting ring. The snap-fitting method may be achieved through elastic buckles, rotational locking, or other mechanical structures to ensure that the top cover fits tightly with the connecting ring when closed, thereby providing an effective light-shielding effect. The material selection of the light-shielding top cover should consider its light-shielding performance and durability, such as black or dark opaque materials.

[0023] As Figure 1 As shown, a device for quickly cleaning brain slices or spinal cord slices multiple times provided by the present application includes a light-shielding top cover 1, an orifice plate 2. Six through holes are provided on the orifice plate 2, and a filter screen 21 is provided at the bottom of the through holes for placing brain slices. Water inlet holes 31 and water outlet holes 32 are respectively provided on both sides of the base 3; the light-shielding top cover 1 is covered on the orifice plate 2, and when the light-shielding top cover 1 is covered on the orifice plate 2, it can also be covered on the base 3; the above-mentioned orifice plate 2 is detachably and embeddedly installed in the base 3, and the bottom is kept connected; there are a switch button and a control panel on the base of the shaker 4, and a base slot 41 is provided on the turntable. Hoses can be connected on both sides of the base slot 41; the base 3 can be snapped into the base slot 41, and the water inlet and outlet are aligned for liquid circulation; the inlet and outlet ends of the peristaltic pump 5 are connected to the water inlet and outlet of the shaker by hoses to drive the liquid circulation of the entire device, and the conical flask 6 can be used to hold the liquid for cleaning or collect waste liquid. The entire system can be set to the liquid storage cleaning mode (the liquid is fed in and then turned off, and then turned on after cleaning) or the flowing circulation cleaning mode (always on) by controlling the switch of the peristaltic pump. The water inlet and outlet speeds of the peristaltic pump can be set according to the different specimens and specific experimental requirements to adjust the water inlet and outlet speeds, so as to ensure that the specimens are fully cleaned on the premise of minimizing damage to the specimens.

[0024] The above content is only to illustrate the technical idea of the present invention, and the protection scope of the present invention cannot be limited thereby. Any modification made on the basis of the technical solution according to the technical idea proposed by the present invention falls within the protection scope of the claims of the present invention.

Claims

1. A device for quickly washing brain slices or spinal cord slices multiple times, characterized in that, It includes a light-shielding top cover (1), an orifice plate (2), a base (3), a shaker (4) and a peristaltic pump (5); the orifice plate (2) is arranged in the base (3), the light-shielding top cover (1) covers the orifice plate (2), multiple through holes are opened on the orifice plate (2), and a filter screen (21) is arranged at the bottom of the through holes; water inlet holes and water outlet holes are opened on opposite sides of the base (3), the water inlet holes and the water outlet holes are connected to the peristaltic pump (5), and a base clamping groove (41) for connecting the base (3) is arranged on the shaker (4).

2. The device for quickly cleaning brain slices or spinal cord slices multiple times according to claim 1, wherein The base (3) is embedded in the base clamping groove (41), through holes are opened at positions of the base clamping groove (41) corresponding to the water inlet hole and the water outlet hole of the base (3), and the peristaltic pump (5) is connected to the water inlet hole and the water outlet hole of the base (3) through a hose passing through the through holes.

3. The device for quickly cleaning brain slices or spinal cord slices multiple times according to claim 1, characterized in that, A control panel and a switch are arranged on the shaker (4).

4. The device for quickly cleaning brain slices or spinal cord slices multiple times according to claim 1, wherein, The peristaltic pump (5) is further connected to a container for holding a liquid for cleaning or collecting waste liquid.

5. The device for quickly washing brain slices or spinal cord slices multiple times according to claim 1, characterized in that, A positioning pin is arranged between the orifice plate (2) and the base (3).

6. The device for quickly washing brain slices or spinal cord slices multiple times according to claim 1, wherein A connecting ring is arranged at the upper end of the base (3), the orifice plate (2) is embedded inside the connecting ring, and the light-shielding top cover (1) is clamped outside the connecting ring.

7. The device for quickly cleaning brain slices or spinal cord slices multiple times according to claim 1, wherein, A handle ring is arranged on the orifice plate (2).

8. The device for quickly washing brain slices or spinal cord slices multiple times according to claim 1, characterized in that, A sealing ring is arranged at the connection between the orifice plate (2) and the base (3).

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