Bacterium collector

Abstract

The bacteria collector comprises a tray, a film pressing piece, a water absorption pad and a bacteria filtering film, the bacteria filtering film comprises a first filtering part, a second filtering part surrounding the periphery of the first filtering part and an installation part surrounding the periphery of the second filtering part, the film pressing piece is connected with the tray in a loosening mode, and the installation part is pressed on the tray through the film pressing piece. The water absorption pad is arranged on the lower side of the first filtering part and can absorb water on the first filtering part, the second filtering part is suspended, and when the film pressing piece is loosened, the bacteria filtering film can be taken out from the tray. The bacteria collector provided by the utility model has the characteristics of simple structure, simplicity in operation, no need of manually applying positive pressure or negative pressure, low use cost, good use effect and wide use range. The experimental method provided by the utility model has low requirements on instruments, consumables and sample size, and has the characteristic of easiness in implementation.

Description

Technical Field

[0001] The utility model relates to the field of experimental technologies, and particularly relates to a bacteria collector. Background Art

[0002] In the prior art, the pour plate method and the membrane filtration method are both common colony counting methods. The pour plate method has the problems of being time-consuming and laborious. The membrane filtration method requires special suction filtration equipment and a supporting suction filtration cup, which has high requirements for instruments and consumables. Or there is a situation where manual pressure is applied with a syringe for filtration, which has the problem of cumbersome operation. And the membrane filtration method has the problems of high consumable cost and large sample demand. Content of the Utility Model

[0003] The purpose of the utility model is to provide a bacteria collector, which helps to alleviate or eliminate at least one of the above technical problems.

[0004] A bacteria collector of the utility model includes a tray, a membrane pressing member, a water absorption pad and a bacteria filtering membrane. The bacteria filtering membrane includes a first filtering part, a second filtering part surrounding the periphery of the first filtering part, and an installation part surrounding the periphery of the second filtering part. The membrane pressing member is detachably connected to the tray, and the membrane pressing member presses the installation part on the tray. The water absorption pad is placed under the first filtering part, and the water absorption pad can naturally absorb the moisture on the first filtering part and the second filtering part. The second filtering part is suspended in the air. When the membrane pressing member is loosened, the bacteria filtering membrane can be taken out from the tray.

[0005] Optionally, a first supporting part and a second supporting part spaced apart and surrounding the periphery of the first supporting part are provided on the tray. The water absorption pad is supported on the first supporting part, and there is a spaced space between the water absorption pad and the second supporting part. The first filtering part covers the water absorption pad, the installation part is pressed on the second supporting part by the membrane pressing member, and the second filtering part is suspended above the spaced space.

[0006] Optionally, a limiting part is provided on the periphery of the first supporting part, and the limiting part and the first supporting part form an installation groove for installing the water absorption pad.

[0007] Optionally, there is a gap between the upper end of the limiting part and the bacteria filtering membrane.

[0008] Optionally, the first filtering part, the second filtering part and the installation part are connected in sequence.

[0009] Optionally, the membrane pressing member is provided with an annular membrane pressing part for pressing the installation part downward.

[0010] Optionally, the film pressing member is detachably connected to the tray.

[0011] Optionally, the tray and the film pressing member are connected to form a cavity with an open upper side, and the bacteria collector further includes a lid for covering the open mouth of the cavity.

[0012] Optionally, the tray includes a tray bottom wall and a tray side wall extending upward from the edge of the tray bottom wall.

[0013] The present utility model also proposes an experimental method, using the bacteria collector described in any one of the above, including the following steps:

[0014] Add the sample liquid to the filter membrane, and spread or shake the sample liquid;

[0015] After the water in the sample liquid on the filter membrane is filtered dry, take out the filter membrane and attach the filter membrane to the surface of the agar medium for cultivation;

[0016] After the cultivation, observe and identify the colonies growing on the filter membrane or count the colonies growing on the filter membrane.

[0017] The bacteria collector proposed by the present utility model has the characteristics of simple structure, simple operation, low use cost, good use effect and wide use range. The experimental method proposed by the present utility model has low requirements for instruments, consumables and sample volume, and has the characteristics of being easy to implement. Description of the Drawings

[0018] Figure 1 It is a cross-sectional view of the bacteria collector described in some embodiments;

[0019] Figure 2 For Figure 1 Partial enlarged view;

[0020] Figure 3 It is an exploded view of the bacteria collector described in some embodiments;

[0021] Figure 4 It is a top view of the tray described in some embodiments;

[0022] Figure 5 It is a schematic diagram of the colonies growing on the filter membrane;

[0023] Figure 6 It is a schematic diagram of the filter membrane attached to the surface of the agar medium for cultivation.

[0024] Wherein: 1 - tray, 2 - water absorption pad, 3 - filter membrane, 4 - film pressing member, 5 - lid, 6 - culture dish, 7 - agar medium, 8 - colonies

[0025] 101 - Tray bottom wall, 102 - Tray side wall, 103 - First support portion, 104 - Second support portion, 105 - Spacing space, 106 - Limiting portion, 107 - Installation groove, 301 - First filtration portion, 302 - Second filtration portion, 303 - Installation portion, 304 - First dotted line, 305 - Second dotted line, 401 - Annular film pressing portion, 402 - Connection section, 403 - Operation section. Detailed implementation manners

[0026] The following will describe the implementation manners of the present invention with reference to the drawings and preferred embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation manners. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be understood that the preferred embodiments are only for explaining the present invention, rather than for limiting the protection scope of the present invention.

[0027] It should be noted that the diagrams provided in the following embodiments only illustrate the basic concept of the present invention in a schematic manner. Therefore, only the components related to the present invention are shown in the diagrams, rather than being drawn according to the number, shape, and size of the components during actual implementation. The type, quantity, and ratio of each component during actual implementation can be arbitrarily changed, and the component layout type may also be more complex.

[0028] As Figures 1 to 5 shown, a bacteria collector includes a tray 1, a film pressing member 4, a water absorption pad 2, and a bacteria filtering membrane 3. The bacteria filtering membrane 3 includes a first filtration portion 301, a second filtration portion 302 surrounding the periphery of the first filtration portion 301, and an installation portion 303 surrounding the periphery of the second filtration portion 302. The film pressing member 4 is detachably connected to the tray 1. The film pressing member 4 presses the installation portion 303 on the tray 1. The water absorption pad 2 is placed under the first filtration portion 301. The water absorption pad 301 can naturally absorb the moisture on the first filtration portion 301 and the second filtration portion 302. The second filtration portion 302 is suspended. When the film pressing member 4 is loosened, the bacteria filtering membrane 3 can be taken out from the tray 1.

[0029] With the above-mentioned bacteria collector, the water-absorbing pad 2 can absorb the moisture of the sample liquid on the first filtering part 301 and the second filtering part 302. The sample liquid on the second filtering part 302 can also be absorbed by the water-absorbing pad. Most of the bacteria in the sample liquid on the second filtering part 302 are retained on the second filtering part 302, so as to prevent excessive sample liquid near the edge of the bacteria-filtering membrane 3 due to the tension effect, resulting in excessive accumulation and non-dispersion of bacteria at the edge, making it difficult to observe and identify. In a short time (usually a few minutes), the moisture of the sample liquid on the first filtering part 301 and the second filtering part 302 can be filtered out, and the bacteria in the sample liquid are intercepted on the first filtering part 301 and the second filtering part 302.

[0030] Since the installation part 303 is pressed on the tray 1 by the film pressing part 4, the tension of the second filtering part 302 near the edge of the installation part 303 on the bacteria-filtering membrane 3 is relatively large, resulting in too much dead corner liquid at the edge of the second filtering part 302 and an increase in the filtering efficiency. If a water-absorbing part is also arranged on the lower side of the second filtering part 302, the filtering efficiency of the second filtering part 302 will be too high, and then it will cause too much sample liquid to be filtered at the second filtering part 302, too many bacteria to be intercepted at the second filtering part 302, and the colonies 8 at the second filtering part 302 will be linearly stacked, usually in a circular linear stack, resulting in difficulty or even impossibility to count the colonies 8. In this embodiment, by arranging the water-absorbing pad 2 on the lower side of the first filtering part 301, the filtering efficiency of the first filtering part 301 can be ensured, and the second filtering part 302 is suspended above the tray 1, which can prevent the filtering efficiency of the second filtering part 302 from being too high, making the filtering efficiencies of the first filtering part 301 and the second filtering part 302 more balanced, making the distribution of bacteria on the bacteria-filtering membrane 3 more uniform during the experiment, and further ensuring that the colonies 8 can be more evenly distributed on the bacteria-filtering membrane 3, preventing the situation of linear stacking of the colonies 8. It is precisely by suspending the second filtering part 302 that the above-mentioned bacteria collector can be actually applied to the experiment.

[0031] In the above-mentioned bacteria collector, by arranging the film pressing part 4, the bacteria-filtering membrane 3 can be tightly pressed on the tray 1, providing convenience for spreading or shaking the sample liquid. The above-mentioned bacteria collector can be applied to the colony counting experiment, especially suitable for the colony counting experiment of 1 ml sample liquid. When conducting the colony counting experiment, add 1 ml of sample liquid to the bacteria-filtering membrane 3, spread or shake the sample liquid; after a short time (usually a few minutes), after the moisture in the sample liquid is absorbed and filtered dry, then the bacteria-filtering membrane 3 can be taken out and the bacteria-filtering membrane 3 can be pasted on the surface of the agar medium 7 for cultivation; after cultivation, the colonies 8 growing on the bacteria-filtering membrane 3 can be counted.

[0032] Using the above-mentioned bacteria collector for colony counting experiments, isolation and culture of target bacteria, and evaluation of disinfection effects (reduction of the number of bacteria on the membrane before and after disinfection), it can replace the cumbersome pour plate method in the prior art, and has the characteristics of simple structure, simple operation, and low use cost.

[0033] The above-mentioned bacteria collector uses the water-absorbing pad 2 to absorb the moisture in the sample liquid. Without applying negative pressure or positive pressure, it uses gravity and microporous capillary action to absorb the moisture in the sample liquid, and has low requirements for instruments, consumables, and sample volume, with the characteristics of cost savings, time savings, easy implementation, and easy operation.

[0034] The above-mentioned bacteria collector filters and collects bacteria using the bacteria-filtering membrane 3, without centrifugation operation, can replace the centrifugation method, has no risk of laboratory pollution and infection caused by aerosols, is more simple and safe, and is conducive to improving the positive rate and detection rate. After bacteria collection, the bacteria-filtering membrane 3 can be translated and attached to the corresponding screening and selective agar medium 7 according to the purpose of bacterial culture inspection. There are various screening and selective media to choose from, such as: screening media for various multi-drug resistant bacteria in various hospital room environments, which greatly improves the positive rate and detection rate, and also greatly expands the detection range.

[0035] The above-mentioned bacteria collector can be used not only for colony counting, but also for screening of special bacterial species. For example, the filter membrane after natural adsorption and filtration of the sample can be attached to SS (screen door-Shigella agar medium) and cultured to achieve the screening of Salmonella and Shigella in food. The above-mentioned bacteria collector can quantitatively add a bacterial suspension to simply and quickly form a quantitative bacterial film for the detection and verification of disinfection effects.

[0036] In some embodiments, a first support portion 103 and a second support portion 104 that is spaced and surrounds the periphery of the first support portion 103 are provided on the tray 1. The water-absorbing pad 2 is supported on the first support portion 103. There is a spaced space 105 between the water-absorbing pad 2 and the second support portion 104. The first filtering portion 301 covers the water-absorbing pad 2, and the installation portion 303 is pressed on the second support portion 104 by the film pressing member 4. The second filtering portion 302 is suspended above the spaced space 105. With the above technical solution, by setting the spaced space 105, the second filtering portion 302 is suspended at the spaced space 105, which can prevent the filtering efficiency of the second filtering portion 302 from being too high, and can prevent the occurrence of liquid deposition and linear accumulation of colonies 8 at the second filtering portion 302.

[0037] In specific implementation, the width of the second filtering portion 302 and the width of the spaced space 105 can be determined based on tests of the actual bacteria-filtering membrane 3, as long as linear accumulation of colonies 8 does not occur at the second filtering portion 302.

[0038] In some embodiments, a limiting portion 106 is provided on the tray 1 and is located outside the first support portion 103. The limiting portion 106 and the first support portion 103 form an installation groove 107 for installing the water absorption pad 2. With the above technical solution, the limiting portion 106 is used to limit the position of the water absorption pad 2 to prevent the water absorption pad 2 from moving. Installing the water absorption pad 2 in the installation groove 107 has the characteristic of being easy to install. As a specific example, the limiting portion 106 is annular, and the inner peripheral surface of the limiting portion 106 cooperates with the outer peripheral surface of the water absorption pad 2. Obviously, the above installation groove 107 is a preferred installation structure for the water absorption pad 2. In specific implementation, other structures capable of installing the water absorption pad 2 can also be adopted on the tray 1.

[0039] As a specific example, there is a gap between the upper end of the limiting portion 106 and the bacteria filtering membrane 3, that is, the upper end of the limiting portion 106 is set lower than the upper surface of the water absorption pad 2, which can prevent the limiting portion 106 from having an adverse effect on the filtering efficiency of the second filtering portion 302 and can prevent the limiting portion 106 from blocking the water absorption capacity of the water absorption pad 2.

[0040] In specific implementation, the first filtering portion 301, the second filtering portion 302, and the installation portion 303 are connected in sequence. In specific implementation, the bacteria filtering membrane 3 is formed by connecting the first filtering portion 301, the second filtering portion 302, and the installation portion 303 in sequence. As Figure 5 shown, the circular portion surrounded by the first dotted line 304 on the bacteria filtering membrane 3 is the first filtering portion 301, the annular portion between the first dotted line 304 and the second dotted line 305 on the bacteria filtering membrane 3 is the second filtering portion 302, and the annular portion outside the second dotted line 305 on the bacteria filtering membrane 3 is the installation portion 303, where the first dotted line 304 and the second dotted line 305 are virtual lines only for better description of the first filtering portion 301, the second filtering portion 302, and the installation portion 303.

[0041] In some embodiments, the pressing film member 4 is provided with an annular pressing film portion 401 for pressing the installation portion 303 downward. Pressing the bacteria filtering membrane 3 by the annular pressing film portion 401 can also prevent the sample liquid on the second filtering portion 302 from leaking outward. It can prevent the sample liquid from leaking outward during the process of spreading or shaking out the sample liquid. In specific implementation, the inner peripheral surface of the annular pressing film portion 401 surrounds the periphery of the second filtering portion 302.

[0042] In some embodiments, the tray 1 includes a tray bottom wall 101 and a tray side wall 102 extending upward from the edge of the tray bottom wall 101. The tray side wall 102 and the tray bottom wall 101 enclose a trough with an open upper side, which can prevent liquid leakage.

[0043] As a specific example, the edge of the bottom wall 101 of the tray is provided with an upwardly convex first annular convex portion, and the bottom wall 101 of the tray is further provided with an upwardly convex second annular convex portion. The first annular convex portion is spaced apart and surrounds the periphery of the second annular convex portion. The tray side wall 102 surrounds the periphery of the first annular convex portion. The first annular convex portion is the second support portion 104, and the second annular convex portion is the limiting portion 106.

[0044] As a specific example, in order to maintain the flatness of the bacteria-filtering membrane, the upper end surface of the second support portion 104 is a horizontally arranged first annular plane, the lower end surface of the film pressing member 4 is a horizontally arranged second annular plane, and the installation portion 303 is clamped between the first annular plane and the second annular plane.

[0045] In some embodiments, the film pressing member 4 is detachably connected to the tray 1. The pressing and loosening of the film pressing member 4 on the bacteria-filtering membrane 3 can be achieved by disassembling and assembling the film pressing member 4, which has the characteristics of being easy to implement and operate. In specific implementation, the detachable connection can adopt but is not limited to snap connection and threaded connection.

[0046] As a preferred example, the film pressing member 4 and the tray side wall 102 can be connected by a threaded connection. During the experiment, the film pressing member 4 can be loosened by rotating the film pressing member 4, which has the characteristic of being easy to operate.

[0047] As a specific example, the film pressing member 4 is a ring-shaped member, and the lower end portion of the film pressing member 4 constitutes an annular film pressing portion 401. More specifically, the film pressing member 4 includes a connecting section 402 and an operating section 403 located above the connecting section 402. The outer peripheral surface of the connecting section 402 is provided with an external thread, and the inner peripheral surface of the tray side wall 102 is provided with an internal thread. The cooperation of the external thread and the internal thread can threadedly connect the film pressing member 4 and the tray side wall 102 together. The lower end portion of the connecting section 402 is the annular film pressing portion 401 for pressing the installation portion 303 of the bacteria-filtering membrane 3. The operating section 403 extends upward out of the tray 1, and the operating section 403 is used for the operator to operate, so that the operator can conveniently rotate the film pressing member 4. The outer diameter of the operating section 403 can be set larger than the outer diameter of the connecting section 402.

[0048] In some embodiments, the tray 1 and the film pressing member 4 are connected to form an upper-side open cavity, and the bacteria collector further includes a lid 5 for covering the open cavity of the cavity. On the one hand, the lid 5 can prevent the bacteria-filtering membrane from being contaminated during transportation or experiment. On the other hand, the lid 5 can prevent the sample liquid from leaking during the process of shaking the sample liquid. As a preferred example, the lid 5 includes a lid top wall and a lid side wall extending downward from the edge of the lid top wall.

[0049] In specific implementation, both the water-absorbing pad 2 and the bacteria-filtering membrane 3 can adopt a circular structure. The diameter of the bacteria-filtering membrane 3 is larger than that of the water-absorbing pad 2, and the diameter of the water-absorbing pad 2 is consistent with the inner diameter of the first annular part. In specific implementation, the bacteria-filtering membrane 3 is provided with filter holes for liquid to pass through and capable of blocking bacteria, which can refer to the bacteria-filtering membranes in the prior art and can directly purchase products sold in the market. The water-absorbing pad 2 can be made of water-absorbing materials, such as blotting paper. The tray 1 and the membrane pressing member 4 can be made of plastic. For example, in order to facilitate observation, the tray 1 and the membrane pressing member 4 are made of transparent plastic.

[0050] The present utility model also proposes an experimental method, which uses the bacteria collector described in any one of the above, and includes the following steps:

[0051] Add an appropriate amount of sample liquid to the first filtering part 301 and the second filtering part 302 of the bacteria-filtering membrane 3, and spread or shake the sample liquid;

[0052] After the moisture in the sample liquid on the bacteria-filtering membrane 3 is absorbed and filtered dry, as Figure 6 shown, take out the bacteria-filtering membrane 3 and attach the bacteria-filtering membrane 3 to the surface of the agar medium 7 for culturing;

[0053] After culturing, as Figure 5 shown, observe and identify the colonies 8 growing on the bacteria-filtering membrane 3 or count the colonies 8 growing on the bacteria-filtering membrane 3.

[0054] In specific operation, the surface of the bacteria-filtering membrane 3 with bacteria faces away from the surface of the agar medium 7, and the agar medium 7 is usually accommodated in the culture dish 6. After attaching the bacteria-filtering membrane 3 to the surface of the agar medium 7, it is usually cultured in an incubator. The amount of the appropriate sample liquid can be 1 ml, or the amount of the sample liquid can be adjusted according to the test requirements.

[0055] The above experimental method has low requirements for instruments, consumables, and the sample volume, and has the characteristics of cost saving, time saving, easy implementation, and easy operation.

[0056] The above embodiments are only preferred embodiments given to fully illustrate the present utility model, and the protection scope of the present utility model is not limited thereto. Equivalent substitutions or transformations made by those skilled in the art on the basis of the present utility model are within the protection scope of the present utility model. In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples", etc. means that the specific features, structures, materials or characteristics of the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine the different embodiments or examples described in this specification.

Claims

1. A bacteria collector, characterized in that, It includes a tray, a film pressing member, a water absorption pad and a bacteria filtering film. The bacteria filtering film includes a first filtering part, a second filtering part surrounding the periphery of the first filtering part, and a mounting part surrounding the periphery of the second filtering part. The film pressing member is releasably connected to the tray, and the film pressing member presses the mounting part onto the tray. The water absorption pad is placed under the first filtering part, and the water absorption pad can naturally absorb the moisture on the first filtering part and the second filtering part. The second filtering part is suspended in the air. When the film pressing member is loosened, the bacteria filtering film can be taken out from the tray.

2. The bacteria collector according to claim 1, characterized in that, The tray is provided with a first supporting part and a second supporting part spaced apart and surrounding the periphery of the first supporting part. The water absorption pad is supported on the first supporting part, and there is a spaced space between the water absorption pad and the second supporting part. The first filtering part covers the water absorption pad, the mounting part is pressed by the film pressing member on the second supporting part, and the second filtering part is suspended above the spaced space.

3. The bacteria collector according to claim 2, wherein, A limiting part is arranged on the periphery of the first supporting part, and the limiting part and the first supporting part form a mounting groove for mounting the water absorption pad.

4. The bacteria collector according to claim 3, characterized in that, There is a gap between the upper end of the limiting part and the bacteria filtering film.

5. The bacteria collector according to claim 1, characterized in that, The first filtering part, the second filtering part and the mounting part are connected in sequence.

6. The bacterium collector according to claim 1, wherein The film pressing member is provided with an annular film pressing part for pressing the mounting part downward.

7. The bacteria collector according to claim 1, wherein The film pressing member is detachably connected to the tray.

8. The bacteria collector according to claim 1, wherein, The tray and the film pressing member are connected to form an open-top cavity, and the bacteria collector further includes a lid for covering the open mouth of the cavity.

9. The bacteria collector according to claim 1, characterized in that, The tray includes a tray bottom wall and a tray side wall extending upward from the edge of the tray bottom wall.

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