Multifunctional universal suspension cell culture device

Abstract

The utility model discloses a multifunctional universal suspension cell culture device which comprises a base, an outer mounting frame and an inner mounting frame located on the inner side of the outer mounting frame are arranged at the top end of the base, and a telescopic water bath heat insulation barrel is arranged in the inner mounting frame; a swing assembly is mounted on the outer mounting frame, the swing assembly is connected with a swing positioning basket arranged in the telescopic water bath heat insulation barrel and drives the swing positioning basket to swing in the telescopic water bath heat insulation barrel through the swing assembly, and a culture bag is mounted in the swing positioning basket; the gas-liquid filtering port cover is provided with a gas inlet pipe connector used for being connected with a gas inlet pipeline, a gas outlet pipe connector used for being connected with a gas outlet pipeline, an inoculation opening used for inoculating cells and a gas detection opening facilitating detection of gas components in the culture bag. According to the utility model, the damage rate of suspension cell culture is effectively reduced, and the pollution rate of suspension cells is also reduced.

Description

Technical Field

[0001] This invention patent relates to the field of cell culture, and particularly to a multi-functional universal suspension cell culture device. Background Art

[0002] Cell culture refers to a method of simulating the in-vivo environment (sterile, appropriate temperature, pH value, and certain nutrient conditions, etc.) in vitro to enable a certain type of cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Whether for the entire bioengineering technology or one of its sub-technologies, namely bio-cloning technology, cell culture is an essential process. Cell culture itself is the large-scale cloning of cells. The cell culture technology can grow a single cell into a large number of simple single cells or minimally differentiated multi-cells through large-scale culture. This is an essential step in cloning technology, and cell culture itself is cell cloning.

[0003] Cell culture technology is classified into three categories: animal cell culture, plant cell culture, and microbial cell culture according to the type of source organism, the technical difficulty and complexity, and the raw materials and reagents such as the culture medium used. Cell culture technology is an important and commonly used technology in the fields of biology and biotechnology industry, medicine and medical technology industry, pharmacology, and pharmaceutical technology. A large number of cells with academic research value and industrial value can be obtained through cell culture. For example: animal cell culture technology can be used to produce virus vaccines, monoclonal antibodies, interferons, etc.; plant cells can be cultured in suspension in a reactor in vitro to produce important substances such as drugs; the aerobic and anaerobic culture technologies of microbial cells can be used to produce vaccines, ferment, and produce high-value substances such as drugs. There are significant differences in the principles, culture media, and processes between animal cell culture, plant cell culture, and microbial cell culture. However, the cell culture devices used and the technologies for providing the growth conditions for cells (such as the culture temperature, cell dispersion state, composition and concentration of the culture gas) adopted by the culture devices have a certain degree of universality. By improving aspects such as the gas distribution method, temperature control method, and sterilization strategy of the culture device, a more efficient, economical, and practical universal cell culture device that can be used to culture microbial cells, animal cells, and plant cells can be obtained.

[0004] The common defects and improvement points of the existing cell culture devices for culturing microbial cells, animal cells, and plant cells will be elaborated separately below.

[0005] 1. Defects and improvement points of the existing animal cell suspension culture device

[0006] Animal cell culture technology can be used to produce viral vaccines, monoclonal antibodies, interferons, etc. In addition, by utilizing the characteristics of cell adhesion growth and contact inhibition in animal cell culture technology, a large number of autologous thin-layer skin cells can be cultured from the healthy skin cells of burn patients for skin grafting. Animal cell culture requires

[0007] a support or agitation. The cell culture of animals in suspension requires an oscillating or stirring device to keep the cells dispersed and suspended in the culture medium; the cell culture of adherent animal cells requires a support; animal cell culture requires

[0008] the addition of animal serum to the culture medium; animal cell culture requires

[0009] Gas exchange such as carbon dioxide and oxygen. Animal cell culture has strict requirements for the gas environment. It has been found that since the carbon dioxide concentration in the air is very low, if cells are not cultured in a carbon dioxide incubator, the carbon dioxide in the culture medium will be depleted, which will affect the normal growth of cells. Therefore, carbon dioxide is both a cell metabolite and a component required by cells, and it is mainly directly related to maintaining the pH of the culture medium. Most animal cells require a slightly alkaline environment with a pH of 7.2 - 7.4, preferably not exceeding 6.8 - 7.6. During cell culture, as the amount of CO2 released increases, the culture medium will become acidic. Therefore, in animal cell culture, NaHCO3 (which forms a buffer pair with H2CO3 formed by the dissolution of CO2 in water), HEPES and other buffer systems are often added to the culture medium to maintain the pH of the culture medium. Usually, if the CO2 concentration in the incubator is set at 5%, the addition amount of NaHCO3 in the culture medium should be 1.97 g / L; if the CO2 concentration is set to maintain 10%, the addition amount should be 3.95 g / L. When the depth of the culture medium exceeds 5 mm, the culture medium needs to be continuously stirred. When it exceeds 10 cm, sterile carbon dioxide and oxygen-containing air also need to be introduced deep into the culture medium to ensure sufficient gas exchange. However, existing ordinary carbon dioxide incubators do not have the function of stirring the culture medium and introducing sterile carbon dioxide and air deep into the culture medium. Although the requirements for controlling carbon dioxide conditions in plant cell culture are not as strict as those in animal cell culture, providing carbon dioxide during plant cell culture can also promote the photosynthesis of plant cells. Another reality that cannot be ignored is that a pure oxygen environment is extremely harmful to animal cells. Therefore, the culture environment for animal cells should be similar to the atmospheric composition. Therefore, the culture of most animal cells still needs to rely on a carbon dioxide incubator that can accurately control the concentrations of oxygen and carbon dioxide and can provide a relatively clean and sterile environment. Therefore, in reality, how to design a suspension animal cell culture device that can meet both the requirement of maintaining an accurate concentration of different gas components in the gas environment above the culture liquid surface and the requirement of introducing sterile and accurately proportioned gas deep into the culture medium is an urgent problem to be solved today.

[0010] In animal cell culture, there is also a very easy-to-pollute problem. In reality, how to design a suspension animal cell culture device that can meet the requirement of reducing cell pollution during suspension cell culture is also an urgent problem to be solved today.

[0011] In addition, most of the cell dispersion components of existing ordinary animal suspension cell culture devices are structures with stirring components. Besides the problem that suspended cells are easily sheared by the stirring structure, resulting in a high damage rate of suspended cell culture, there are also problems of low flexibility in use and easy contamination. In response to these various problems, one of the solutions urgently needed to address these defects is to design a culture device that can not only meet the aforementioned requirements but also reduce the damage rate of suspended cell culture and the contamination rate.

[0012] 2. Defects and improvable points of existing plant cell suspension culture devices

[0013] Compared with animal cells, the principle of the requirement for hormones in the in vitro culture of plant cells has been relatively well understood, and its application technology has also been quite mature. There is already a set of usable culture media, and at the same time, the problems of the requirements of plant cells for water, nutrients, hormones, osmotic pressure, pH, trace elements, etc. have been solved. The principle of plant cell culture is the totipotency of plant cells. Generally, new plant individuals can be obtained after culture. Therefore, the process of plant tissue culture is dedifferentiation and redifferentiation, which is different from the process of animal cell culture, namely primary culture and subculture. Plant cell culture requires (1) plant auxin - plant hormone. (2) Light. Light is not only related to photosynthesis but also related to cell differentiation. Therefore, in the early stage of plant cell culture aimed at obtaining plants, light conditions are particularly important. For example, the photoperiod can regulate the differentiation of sex cells and flowering; however, the light conditions for in vitro cultured plant cells are not very strict because the substances required for their cell growth mainly rely on the culture medium. Plant cell culture can use solid media or liquid media for suspension culture. However, in the process of obtaining important substances, such as producing drugs, by in vitro culture of plant cells, most are carried out in suspension culture in a reactor. Since the oxygen demand of plant cell suspension is relatively low, the precipitation rate in the culture medium is relatively fast, and it is more easily damaged by the shear force of the stirring device. The existing plant suspension cell culture devices adopt a fixed structure with an internal stirring component and an internal temperature control component. These devices are not isolated from the cultured cells and are in direct contact with the cultured cells. The stirring components and temperature control components of this type of culture device have low flexibility in use and are prone to causing cell damage. During repeated sterilization operations, it is easy to cause contamination of the culture with miscellaneous bacteria due to incomplete sterilization. How to design a plant suspension cell culture device that can reduce the shear force damage of suspended plant cell culture and the contamination rate is the problem to be solved at present.

[0014] 3. Defects and improvable points of existing microbial cell suspension culture devices

[0015] Most microorganisms are single-celled organisms, and the conditions for their survival and the culture medium are relatively simple. Therefore, microbial cell culture technology is simpler than animal and plant cell culture in terms of the culture process and the composition of the culture medium. However, when it comes to aseptic pure culture, in terms of the requirements for culture equipment, the microbial fermentation culture device is as complex as the animal and plant cell culture devices. In addition, the culture of microorganisms has strict requirements for cleanliness, culture gas, temperature, etc. When culturing aerobic microorganisms, sterile oxygen-containing air needs to be provided and the culture solution needs to be continuously stirred. When culturing anaerobic microorganisms, a more complex device is required than for aerobic microorganism culture. Strict anaerobic culture requires maintaining the concentration of inert gases such as carbon dioxide. For example, when producing anaerobic bacteria vaccine products, it is necessary to strictly remove the oxygen component and continuously maintain the concentration of inert gases such as nitrogen and carbon dioxide. In addition, when culturing microorganisms for industrial waste gas treatment, it is necessary to introduce a domestication gas with a precisely controlled concentration. However, the existing microbial culture devices lack a diversified culture gas distribution system, and their stirring components, temperature control components, and the culture container itself need to be sterilized repeatedly. During repeated sterilization operations, cell contamination is likely to occur due to incomplete sterilization. How to design a microbial cell culture device that can accurately provide diversified culture gases and reduce the contamination rate is an urgent problem to be solved currently. Summary of the Invention

[0016] The purpose of this invention patent is to provide a multi-functional universal suspension cell culture device, which can provide precise gas distribution to meet the requirements of different types of cells for culture gas; the device can avoid damage to suspension cells caused by the stirring effect and can also reduce the probability of biological contamination caused by incomplete sterilization of the device.

[0017] To solve the above-mentioned technical problems, this invention patent provides a multi-functional universal suspension cell culture device, including a base. At the top of the base, there is an outer mounting frame and an inner mounting frame located inside the outer mounting frame. Inside the inner mounting frame, there is a telescopic water bath heat insulation barrel.

[0018] A swing component is installed on the outer mounting frame. The swing component is connected to a swing positioning basket arranged inside the telescopic water bath heat insulation barrel and drives the swing positioning basket to swing inside the telescopic water bath heat insulation barrel through the swing component. A culture bag is installed inside the swing positioning basket. The upper part of the culture bag is connected to a gas-liquid filtration port cover. The gas-liquid filtration port cover is installed on an inverted conical supporting bearing. Therefore, the culture bag, the gas-liquid filtration port cover, and the paddle blades inside the culture bag can swing within a certain angle range with the center of the inverted conical supporting bearing as the center. The inverted conical supporting bearing is installed on a first support plate. The first support plate is installed on the outer mounting frame. At the top of the outer mounting frame, there is also a second support plate installed and connected, which is parallel to the first support plate.

[0019] The gas-liquid filtration port cover is provided with an intake pipe interface for connecting an intake pipe, an outlet pipe interface for connecting an outlet pipe, an inoculation port for inoculating cells, and a gas detection port for facilitating the detection of the gas components inside the culture bag. Among them, sterilization filters are installed at both the intake pipe interface and the outlet pipe interface, and a detection port valve is installed at the top of the gas detection port.

[0020] Furthermore, lifting rollers are evenly spaced and fixed along the circumference at the bottom end of the base. A heat insulation plate is arranged at the top end of the base, and a groove matching with the bottom end of the telescopic water bath heat insulation barrel is opened at the middle position of the top end of the heat insulation plate.

[0021] Furthermore, the outer mounting frame includes outer columns evenly spaced along the circumference at the top end of the circular base. The outer columns include first vertical sleeves sleeved successively from bottom to top. The lower end of the lowermost first vertical sleeve in the outer columns is sleeved on the upper end of the first fixed pipe, and the first fixed pipe is vertically fixed on the base;

[0022] Horizontal centripetal trustees are installed at both the upper and lower ends of any of the first vertical sleeves.

[0023] The lower end of the uppermost first vertical sleeve in the outer columns has a trustee to support and connect to the bottom surface of the first support disk, and the upper end of the uppermost first vertical sleeve in the outer columns has a trustee to support and connect to the bottom surface of the second support disk.

[0024] Furthermore, the inner mounting frame includes inner columns evenly spaced along the circumference at the top end of the base. The inner columns include second vertical sleeves sleeved successively from bottom to top. The lower end of the lowermost second vertical sleeve in the inner columns is sleeved on the upper end of the second fixed pipe, and the second fixed pipe is vertically fixed on the base;

[0025] Centripetal fixing rings are installed on the side of any of the second vertical sleeves.

[0026] Furthermore, the telescopic water bath heat insulation barrel is a double-layer foldable heat preservation barrel. The telescopic water bath heat insulation barrel can be made of materials such as rubber and plastic. It is provided with two layers of barrel walls, and an inflatable heat preservation interlayer is formed between the two barrel walls. The two barrel walls can be folded simultaneously, so the height of the water bath heat insulation barrel wall and its interlayer wall can be adjusted, and the volume of the heat insulation barrel and the heat preservation interlayer can be adjusted. An inflation port communicating with the inside of the heat preservation interlayer is opened at the top end of the telescopic water bath heat insulation barrel, and the inflation port is sealed with a detachable threaded rubber plug. The cross-sectional view of the foldable area of the inner and outer two-layer barrel walls presents a serrated shape. The serrations in the foldable area are divided into two areas: small tooth area and large tooth area. The small tooth area on the outer barrel wall can be closely matched with the aluminum alloy barrel clamp and the barrel hoop assembly. The small tooth area on the outer barrel wall is connected with a U-shaped positioning bolt matching with the barrel hoop.

[0027] Circular aluminum alloy barrel clamp and barrel hoop assemblies are installed at the upper, middle and lower positions on the outer side of the outer barrel wall of the telescopic water bath insulation barrel, and the inner ring layer of the aluminum alloy barrel clamp and barrel hoop assembly is provided with a serrated cavity that tightly matches the small tooth area of the outer wall of the telescopic water bath insulation barrel. Positioning holes are evenly spaced and concentrically opened on the side of the aluminum alloy barrel clamp and barrel hoop assembly in the circumferential direction, and the positioning holes cooperate with the U-shaped positioning bolts in the small tooth area of the outer barrel wall. Connecting rings are evenly spaced along the circumferential direction on the outer side of the aluminum alloy barrel clamp and barrel hoop assembly, and the connecting rings can be connected with the fixing rings of the corresponding height on the inner mounting frame;

[0028] At the top of the telescopic water bath insulation barrel, close to the outside of the gas-liquid filter port cover, there is a foam plastic insulation cover composed of two semicircular foam insulation plastics to reduce the temperature fluctuation in the water bath barrel. There is a candied haws-shaped opening on the surface of the insulation cover to facilitate the swing of the swing arm of the swing frame.

[0029] Furthermore, the swing assembly includes a swing positioning basket assembly and a swing frame assembly. The swing frame assembly drives the swing positioning basket and the culture bag to swing. One of the components of the swing frame assembly is a swing ring, which is composed of two semicircular rings. The side of the swing ring is connected to the lower end of a vertical force transmission rod vertically arranged inside the telescopic water bath insulation barrel. Several full-spectrum instrument spotlights are installed in the middle and upper part of the vertical force transmission rod as a light source for lighting photosynthetic bacteria, plant cells, etc., and can also be used as a light source for observing the growth status of the culture in the culture bag. The upper end of the vertical force transmission rod is fixedly connected to one end of the horizontal force transmission rod, and the other end of the horizontal force transmission rod is rotatably connected to the upper end of the rocker arm. The lower end of the rocker arm is connected to the output shaft of the motor, and the motor is fixed on the external mounting frame. The swing frame assembly shown in the figure is a schematic diagram of the appearance. In actual use, its installation position is: the swing frame assembly in the illustrated position is rotated 90 degrees in the clockwise direction above the plane of the base, and then installed.

[0030] Furthermore, the swing positioning basket assembly includes a positioning basket, the outer side of the positioning basket is evenly provided with ears, the top side of the positioning basket is evenly provided with upper ears, the two upper ears facing each other are passed through by a lifting rope to form a handle shape, the lifting rope is called a handle, and the middle of the handle is hung on the lower side hanging ring of the inverted cone support bearing.

[0031] Further, the sterile culture bag includes an annular rigid bag neck, a bag neck sealing film, a double-layer rigid bag shoulder, a soft bag body, a double-layer rigid bag bottom, a first paddle shaft and paddle blades, and a second paddle shaft and paddle blades. Different parts of this transparent bottle-shaped single-use sterile culture bag exhibit rigid, semi-rigid, and soft states respectively due to different wall thicknesses. The annular rigid bag neck is made of a relatively hard, high-rigidity, and thick material, and a transparent resin material can be used; the combination method of the double-layer rigid bag shoulder, the double-layer rigid bag bottom and the soft bag body is that two relatively hard, high-rigidity, and thick materials sandwich the corresponding parts of the soft bag body in the middle and are combined into one body by hot melt pressing / bonding; the paddle shaft is made of a material with slightly greater rigidity, such as a relatively hard resin material; the paddle blades are made of a semi-hard and slightly soft material. For organisms that require strict sterile culture, single-use culture bags that are not reused should be required. The sterile fermentation culture of single-time suspended cells can be carried out independently in a culture bag, so it is not necessary to sterilize the culture container before each cell culture, and the problem of cross-contamination caused by incomplete sterilization of traditional culture equipment will not occur, and energy, manpower, and time are saved, and the production efficiency is improved.

[0032] Further, an air extraction port for extracting oxygen inside the culture bag is also opened on the gas-liquid filtration port cover;

[0033] An air inlet long tube is connected to the lower side of the gas-liquid filtration port cover. The upper end of the air inlet long tube is communicated with the air inlet pipe interface, the lower end of the air inlet long tube extends to the lower side inside the culture bag, and a shower head is connected to the outlet position of the lower end of the air inlet long tube.

[0034] Further, a gas distribution component is installed on the second support plate. The gas distribution component includes a number of gas distribution pipes communicated with the air inlet pipe interface, a gas distribution exhaust pipe communicated with the air outlet pipe interface, and a gas distribution main machine for controlling the opening and closing of the corresponding pipelines;

[0035] A gas distribution sterilization filter is arranged at one end of the gas distribution pipe away from the connection with the air inlet pipe interface, and a gas distribution valve and a flow meter are connected to the pipeline of the gas distribution pipe;

[0036] A control valve is connected to the pipeline of the gas distribution exhaust pipe.

[0037] Further, a gas detection component is installed on the second support plate. The gas detection component includes a probe tube communicated with the gas detection port. The top end of the probe tube is connected with a gas detection main machine, and the lower end of the gas detection main machine is connected with a gas detection probe extending into the probe tube.

[0038] Further, an anaerobic generating assembly is installed on the second support disk. The anaerobic generating assembly includes an anaerobic gas guiding pipe communicated with the air guiding port. The other end of the anaerobic gas guiding pipe extends into the interior of the glass cover. An electronic igniter is arranged inside the glass cover. The electronic igniter is connected to an external gas source through a gas delivery pipe;

[0039] An anaerobic generating filter is installed on the pipeline of the anaerobic gas guiding pipe. The top of the glass cover is connected with an anaerobic gas outlet pipe, and an anaerobic gas outlet valve is connected to the pipeline of the anaerobic gas outlet pipe.

[0040] Further, a temperature control assembly is installed on the second support disk. The temperature control assembly includes a temperature controller installed at the top end of a temperature-controlled heating protection cover. The bottom end of the temperature-controlled heating protection cover extends downward into the interior of the telescopic water bath heat insulation barrel;

[0041] The lower end of the temperature controller is connected with a stirring paddle, a temperature measuring probe and a heating pipe extending into the temperature-controlled heating protection cover.

[0042] Further, an installation disk for installing each control module of the control system is installed on the second support disk. The control system includes a central control module for setting the whole machine culture mode and coordinating other modules, a mixing module for controlling the swing assembly to swing, a gas distribution quantitative delivery module for controlling the gas distribution assembly to quantitatively deliver specified gases, a gas component monitoring module for cooperating with the gas detection assembly to monitor gas components, an anaerobic control module for controlling the anaerobic generating assembly to burn oxygen, and a culture temperature control module for controlling the temperature control assembly to keep the water bath temperature inside the telescopic water bath heat insulation barrel constant.

[0043] Compared with the prior art, the present invention has at least the following beneficial effects:

[0044] The present invention can provide and flexibly switch various types of culture gases, and precisely adjust the concentration of the culture gases to realize various culture modes, such as anaerobic mode, aerobic mode, carbon dioxide incubator mode, culture mode of microorganisms metabolizing and treating specific gases, photosynthetic cell culture mode, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 is the main view sectional view of the multifunctional universal suspension cell culture device of the present invention.

[0046] Figure 2 is the schematic diagram of the assembly structure of the inner and outer mounting frames of the multifunctional universal suspension cell culture device of the present invention.

[0047] Figure 3 is the schematic diagram of the main structure section of the telescopic water bath heat insulation barrel of the multifunctional universal suspension cell culture device of the present invention.

[0048] Figure 4 It is a schematic diagram of the aluminum alloy barrel clamp at the upper barrel edge of the multi-functional universal suspension cell culture device of this invention patent.

[0049] Figure 5 It is a schematic diagram of the aluminum alloy barrel hoop of the multi-functional universal suspension cell culture device of this invention patent.

[0050] Figure 6 a and 6b are respectively the front view and top view of the schematic diagram of the swing assembly of the multi-functional universal suspension cell culture device of this invention patent. During actual use, its installation position is: install the swing frame assembly shown in the top view at a position rotated 90 degrees clockwise around the center line of the base.

[0051] Figure 7 It is a schematic diagram of the swing positioning basket assembly of the multi-functional universal suspension cell culture device of this invention patent.

[0052] Figure 8 It is a schematic diagram of the sterile culture bag of the multi-functional universal suspension cell culture device of this invention patent.

[0053] Figure 9 It is the top view of the gas-liquid filtration port cover of the multi-functional universal suspension cell culture device of this invention patent.

[0054] Figure 10 It is the sectional view of the front view of the gas-liquid filtration port cover of the multi-functional universal suspension cell culture device of this invention patent.

[0055] Figure 11 It is the schematic diagram of the gas distribution assembly and each module of the control system of the multi-functional universal suspension cell culture device of this invention patent.

[0056] Figure 12 It is a schematic diagram of the gas detection component of the multi-functional universal suspension cell culture device of this invention patent.

[0057] Figure 13 It is a schematic diagram of the anaerobic generation component of the multi-functional universal suspension cell culture device of this invention patent.

[0058] Figure 14 It is the front view of the constant temperature component of the multi-functional universal suspension cell culture device of this invention patent.

[0059] Figure 15 It is a schematic diagram of the internal structure of the constant temperature component of the multi-functional universal suspension cell culture device of this invention patent.

[0060] Figure 16 It is a schematic diagram of the first support plate 10 of the multi-functional universal suspension cell culture device of this invention patent.

[0061] Figure 17 It is a schematic diagram of the second support plate 11 of the multi-functional general suspension cell culture device of the present invention patent.

[0062] Figure 18 It is a schematic diagram of the foamed plastic heat insulation cover 19 of the multi-functional general suspension cell culture device of the present invention patent. Specific embodiments

[0063] The multi-functional general suspension cell culture device of the present invention patent will be described in more detail below in conjunction with the schematic diagrams, in which the preferred embodiments of the present invention patent are shown. It should be understood that those skilled in the art can modify the present invention patent described herein while still achieving the advantageous effects of the present invention patent. Therefore, the following description should be understood as broad knowledge for those skilled in the art and not as a limitation to the present invention patent.

[0064] In the following paragraphs, the present invention patent will be described more specifically by way of example with reference to the accompanying drawings. The advantages and features of the present invention patent will be clearer according to the following description and the claims. It should be noted that the drawings are all in a very simplified form and use non-precise scales, only for the purpose of conveniently and clearly assisting in explaining the purpose of the embodiments of the present invention patent.

[0065] Please refer to Figure 1 , a suspension cell culture device, comprising a circular base 1, the base 1 is composed of lifting rollers 101, a circular chassis 105, a first fixing tube 103, a second fixing tube 102, and a heat insulation plate 104. The bottom end of the circular chassis 105 is fixedly provided with lifting rollers 101 at uniform intervals along the circumference. The provision of lifting rollers 101 at the bottom end of the base 1 enables the entire device to be easily movable and can quickly adapt to the usage requirements at different positions in the production site. The top end of the circular chassis 105 is welded with a first fixing tube 103 and a second fixing tube 102. A hot plate 104 is provided at the top end of the circular chassis 105. Through holes for the first fixing tube 103 and the second fixing tube 102 to pass through are provided in the circumference of the plate body of the heat insulation plate 104. A groove is provided in the middle position at the top end of the heat insulation plate 104. The heat insulation plate 104 is provided at the top end of the base 1 to cooperate with the bottom end of the telescopic water bath heat insulation barrel 5. On the one hand, it increases the stability of the telescopic water bath heat insulation barrel 5 after installation, and on the other hand, it reduces the heat dissipation at the bottom of the telescopic water bath heat insulation barrel 5 and slows down the temperature dissipation rate of the water bath inside the telescopic water bath heat insulation barrel 5 per unit time, thereby increasing the constant temperature effect of the telescopic water bath heat insulation barrel 5. An outer mounting frame and an inner mounting frame located inside the outer mounting frame are provided at the top end of the base 1, and a telescopic water bath heat insulation barrel 5 is provided inside the inner mounting frame, providing the basic composition structure of the device.

[0066] An oscillating frame assembly 6 is installed on the outer mounting frame. The oscillating frame assembly 6 is connected to an oscillating positioning basket assembly 7 disposed inside the telescopic water bath heat insulation barrel 5 and drives the oscillating positioning basket assembly 7 and the sterile culture bag 8 to swing inside the telescopic water bath heat insulation barrel 5 through the oscillating frame assembly 6. The sterile culture bag 8 is suspended inside the oscillating positioning basket assembly 7. The sterile culture bag 8 is used to replace the traditional fermenter to provide a culture space for cells. It has the characteristics of low cost, small weight and flexible use. The hard bag neck 807 of the sterile culture bag 8 is hermetically connected to the gas-liquid filtration port cover 9 fixed on the first support disk 10 by means of screw thread engagement. The bag body 801 and other components of the sterile culture bag 8 below the hard bag neck can swing inside the telescopic water bath heat insulation barrel 5 driven by the oscillating frame assembly 6. The suspended cell culture inside the culture bag 8 can be shaken and mixed under the action of swinging, thereby increasing the culture efficiency of suspended cells. The oscillating frame assembly 6 is used to drive the culture bag 8 to swing to achieve the mixing of the suspended cell culture substances inside the culture bag 8. Compared with setting up a stirring mechanism for mixing, there is no structure or component in the sterile culture bag 8 that causes cell shearing due to continuous rotation, and the shearing force of the stirring structure on the suspended cell culture substances is reduced, the damage rate and pollution rate of the suspended cell culture are reduced, and the culture success rate of suspended cells is increased.

[0067] Among them, the first support disk 10 is a disk-shaped mounting support disk welded by circular steel pipes, and there is an installation position 1001 for the component - reverse chasing bearing 12 on it. The reverse chasing bearing 12 is used to install the sterile culture bag port cover 9. Before all the components on the first support disk 10 are installed and in place, it can rotate slightly on the bracket to adjust the horizontal position of the installation positions of the components on it. After all the components are installed and in place, the first support disk 10 is positioned on the centripetal support tube at the lower end of the uppermost first vertical sleeve 3 of the outer column with screws and nuts. There is a temporary installation position on the first support disk 10 for use when overhauling the component 12, and a pipeline arrangement ring 1002 for arranging and placing the air supply pipeline. There are relatively large gaps on the first support disk 10 to facilitate the passage of components such as the sterile culture bag 8, so as to conveniently adjust each component to the corresponding installation position. Figure 16 It is a schematic structural diagram of the first support disk 10 of the multi-functional suspended cell culture device of the present invention patent.

[0068] Among them, the second support plate 11 is a disc-shaped one welded by circular steel pipes, with installation positions 1101 for the control system installation plate 18, anaerobic generation device installation position 1102, and external type constant temperature device installation position 1103 on it. The second support plate 11 can rotate slightly on the bracket to adjust the horizontal positions of the installation positions of the components on it. There are also air supply pipeline organizing rings for organizing the air supply pipelines and annular temporary maintenance positions for temporarily placing components in scenarios such as equipment maintenance on the second support plate 11. After each component is installed in place, the second support plate 11 is fixed to the centripetal support on the upper end of the uppermost first vertical sleeve 3 of the outer column with screws and nuts. Figure 17 It is a schematic structural diagram of the second support plate 11 of the multifunctional general-purpose suspension cell culture device of the present invention patent.

[0069] The components of the single-use sterile culture bag 8 are a transparent bottle-shaped culture bag that has been aseptically treated and has the function of providing a space for the sterile culture bag. It is composed of components such as an annular hard bag neck 806, a bag neck sealing film 805, a double-layer hard bag shoulder 807, a soft bag body 801, a double-layer hard bag bottom 804, a first paddle shaft and paddle blades 802, a second paddle shaft and paddle blades 803, etc. The single-use sterile culture bag is made by splicing and combining transparent material components with different hardnesses, thicknesses, and shapes through processes such as hot melt pressing and bonding, and transparent polymer materials such as resins can be used. The first paddle shaft and paddle blades 802 and the second paddle shaft and paddle blades 803 are installed inside the inner hard bag bottom of the double-layer hard bag bottom 804. The outside of the inner hard bag bottom is wrapped by the bottom of the soft bag body 801, and the outer hard bag bottom wraps the outside of the bottom of the soft bag body. The bottom of the soft bag body is sandwiched between the inner and outer layers of the double-layer hard bag bottom 804 and integrated in a hot melt pressing / bonding manner. The inner and outer layers of the double-layer hard bag shoulder 807 sandwich the upper and middle parts of the soft bag body 801 and are integrated in a hot melt pressing / bonding manner. The part where the edge of the soft bag body 801 extends out of the hard bag shoulder 807 becomes the bag neck sealing film. The annular hard bag neck 806 is thermally melted and connected to the inner layer of the double-layer hard bag shoulder 807, and a screw thread is engraved on the inner circle of the annular hard bag neck 806 for screwing the gas-liquid filtration port cover 9.

[0070] The top annular hard bag neck 806 of the culture bag 8 is threadedly sealed and connected to the gas-liquid filtration port cover 9, and is hermetically connected to the gas-liquid filtration port cover sealing film 908 with the bag neck sealing film 805. The gas-liquid filtration port cover 9 is installed above the first support plate 10. The first support plate 10 of the uppermost first vertical sleeve is installed on the outer mounting frame, and a second support plate 11 parallel to the first support plate 10 is connected to the top of the outer mounting frame vertically above the first support plate 10. The gas-liquid filtration port cover 9 and all components directly connected thereto are sterile components.

[0071] Please refer to Figure 9 and Figure 10, a plurality of air inlet pipe interfaces 901 for connecting the long air inlet pipe 906 of the inner bag pipe, an anaerobic mode air extraction port 902, a plurality of air outlet pipe interfaces 905 for connecting the short air outlet pipe 907 of the inner bag pipe, an inoculation port 903 for adding sterile culture medium and inoculating cells, and a gas detection port 904 for detecting the culture gas inside the culture bag 8 under positive pressure are provided on the gas-liquid filtration port cover 9. There is a gas-liquid filtration port cover sealing film 908 at the lower part of the gas-liquid filtration port cover. Filter sterilizers are installed on the upper parts of the air inlet pipe interface 901, the anaerobic mode air extraction port 902, the inoculation port 903, the gas detection port 904, and the air outlet pipe interface 905. A gas detection port valve 910 is also installed on the top of the gas detection port 904, and the gas detection port valve 910 is connected to a relatively large-diameter gas detection port filter sterilizer 909. These ventilation ports, inoculation ports, and detection ports provided on the gas-liquid filtration port cover 9 facilitate the cultivation of cells in different environments.

[0072] Please refer to Figures 1 to 2 , the outer mounting frame includes outer columns evenly spaced along the circumference of the top end of the circular base 1. The outer columns include first vertical sleeves 3 sleeved on each other from bottom to top. The lower end of the lowermost first vertical sleeve 3 in the outer column is sleeved on the upper end of the first fixed pipe 103, and the first fixed pipe 103 is vertically fixed on the base 1. Centripetal trustees 301 and 302 are horizontally installed at the upper and lower ends of any first vertical sleeve 3 respectively; the horizontally centripetal trustee 302 at the lower end of the first vertical sleeve 3 of the uppermost outer column supports and connects to the bottom side of the circular first support disc 10, and the trustee 301 at the upper end of the first vertical sleeve at the top of the uppermost outer column is used to support and connect to the bottom surface of the circular second support disc 11.

[0073] Specifically, the outer mounting frame is composed of spliced outer columns evenly arranged on the base 1. The number of first vertical sleeves 3 in the outer columns can be determined according to the height requirements of the device during actual use to change the height of the outer columns, so as to adjust the height of the outer mounting frame. After the outer columns are formed, the trustees 302 and 301 can provide a supporting surface to provide an installation position for installing the circular first support disc 10 and the second support disc 11 at the top of the device.

[0074] Furthermore, the inner mounting frame includes inner columns evenly spaced along the circumference of the top end of the base 1. The inner columns include second vertical sleeves 2 sleeved on each other from bottom to top. The lower end of the lowermost second vertical sleeve 2 in the inner column is sleeved on the upper end of the second fixed pipe 102, and the second fixed pipe 102 is vertically fixed on the base 1; a centripetal spring buckle fixing ring 201 is installed on the side of any second vertical sleeve 2. In addition, there is an adjusting pipe 200 without a spring buckle fixing ring and only used for adjusting the height.

[0075] Specifically, the inner mounting frame is composed of spliced inner columns evenly spaced on the bottom height base 1. The number of the second vertical sleeves 2 in the inner columns can be determined according to the actual use height requirement of the device to change the height of the inner columns, so as to change the height of the inner mounting frame to adapt to the telescopic water bath heat insulation barrel 5 after different depth adjustments. And a connection position can be provided by the spring buckle fixing ring 201 after the inner columns are formed. After the subsequent telescopic water bath heat insulation barrel 5 is installed inside the inner mounting frame formed by the inner columns, the telescopic water bath heat insulation barrel 5 can be fixed inside the inner mounting frame through the structural cooperation between the spring buckle fixing ring 201 and the telescopic water bath heat insulation barrel 5.

[0076] Specifically, the telescopic water bath heat insulation barrel 5 is provided with a double-layer barrel wall 501 to form an inflatable heat preservation interlayer 502, which can increase the heat preservation ability of the telescopic water bath heat insulation barrel 5 while ensuring the telescopic function of the telescopic water bath heat insulation barrel 5, and adapt to provide a constant temperature environment for suspension cell culture with adjustable volume.

[0077] Please refer to Figures 1 to 5 , the telescopic water bath heat insulation barrel 5 is provided with two layers of foldable barrel walls 501. The barrel walls are in a foldable serrated shape, divided into small tooth areas and large tooth areas. Among them, U-shaped positioning bolts 504 are evenly spaced along the circumference in the small tooth area of the outer barrel wall 501. The U-shaped positioning bolts 504 are used to cooperate with and pass through the positioning holes on the side of the aluminum alloy barrel clamp and the barrel hoop assembly 15. A heat preservation interlayer 502 is formed between the two layers of barrel walls 501. The top end of the telescopic water bath heat insulation barrel 5 is a thicker upper barrel edge, and the bottom end is a thicker lower barrel edge. An air inlet communicating with the inside of the heat preservation interlayer 502 is opened at the middle part of the circumference of the upper barrel edge. The air inlet is engraved with threads and is screwed with a sealing plug 503. Tiny screw through holes are vertically drilled evenly on the outer circumferential part of the upper barrel edge that is not communicated with the heat preservation interlayer.

[0078] Furthermore, aluminum alloy barrel clamps and barrel hoop assemblies 15 are installed on the upper barrel edge and the outer wall of the barrel wall 501 of the telescopic water bath heat insulation barrel 5. The aluminum alloy barrel clamps and barrel hoop assemblies 15 include a set of upper barrel edge upper clamping rings 1501, a set of upper barrel edge lower clamping rings 1502, and two sets of barrel hoops 1503. The upper barrel edge upper clamping ring 1501 is a circular ring piece formed by two semi-circular aluminum alloys assembled on the upper part of the upper barrel edge; the upper barrel edge lower clamping ring 1502 is two semi-circular aluminum alloys assembled on the lower part of the upper barrel edge and cooperating with the small tooth area on the outer wall of the barrel wall close to the lower part of the barrel edge. The two upper barrel edge upper clamping rings 1501 are butted at the rib plates at the ends of the semi-ring bodies and assembled into a ring with screws and nuts. The two upper barrel edge lower clamping rings 1502 are butted at the rib plates at the ends of the semi-ring bodies and assembled into a ring with screws and nuts. Then, the assembled upper barrel edge upper clamping ring 1501 and upper barrel edge lower clamping ring 1502 are wrapped around the outside of the upper barrel edge, and the screw holes on the outer edges of the two clamping rings are aligned and fixed with screws and nuts. The inner ring side surfaces of the ring bodies of the upper barrel edge lower clamping ring 1502, the two sets of barrel hoops 1503, all have tooth groove areas that cooperate with the small tooth areas of the telescopic water bath heat insulation barrel. In addition, positioning holes are evenly spaced circumferentially and centripetally in the tooth groove area, and the positioning holes cooperate with the U-shaped positioning bolts 504 on the outer cylindrical wall of the barrel wall 501 of the telescopic water bath heat insulation barrel. The U-shaped positioning bolts 504 pass through the positioning holes in the corresponding tooth groove areas to connect the spring movable rings and fix the ring bodies on the brackets, that is, the fixing rings 201 of the second vertical sleeve 2 at the corresponding heights. The two sets of barrel hoops 1503, and the fixing rings welded to the outer ring side surfaces of the outer ring side edges of the outer ring body of a set of upper barrel edge lower clamping rings 1502 have connecting rings for connecting the spring movable rings and fixing the ring bodies on the fixing rings 201 of the second vertical sleeve 2 at the corresponding heights.

[0079] Please refer to Figure 18 , at the top of the telescopic water bath heat insulation barrel described above, near the outer side of the gas-liquid filtration port cover, there is a foam plastic heat insulation cover 19 formed by two semi-circular foam plastic heat insulations. There are three-layer openings in the shape of candied haws with long and narrow steps on its surface to facilitate the swinging of the swing rod of the swing frame. In the non-touch area of the swing rod, more miniature foam plastic round covers 1901, 1902, 1903 with different diameters are equipped. On the premise of not affecting the swinging of the swing rod of the swing frame, the above more miniature foam plastic round covers can be selected to cover for heat insulation.

[0080] Please refer to Figure 1 and Figure 6, the swing frame assembly 6 embraces and surrounds the outside of the swing positioning basket assembly 7, driving the swing positioning basket assembly 7 and the sterile culture bag 8 inside it to swing. The swing frame assembly 6 contains the following accessories, and is assembled in the following manner: a swing ring 605 formed by two semicircular rings, the side of the swing ring 605 is connected to the lower end of a vertical force transmission rod 604 vertically arranged inside the telescopic water bath insulation barrel 5, the upper end of the vertical force transmission rod 604 is fixedly connected to one end of the horizontal force transmission rod 603, the other end of the horizontal force transmission rod 603 is rotatably connected to the upper end of the rocker arm 602, and the lower end of the rocker arm 602 is fixed to the output shaft of the swing frame motor 601. The swing frame assembly shown in the figure is a schematic diagram of the appearance. During actual installation and use, the installation position of the swing frame assembly 6 needs to be Figure 6 b The top view shows that the swing frame assembly 6 is rotated 90 degrees in the clockwise direction.

[0081] Specifically, the swing frame motor 601 is fixed on the external mounting frame, and the swing frame motor 601 provides the swing power. After the swing frame motor 601 is started, the output shaft of the swing frame motor 601 drives the rocker arm 602 to rotate. The rotation of the rocker arm 602 can drive the horizontal force transmission rod 603 connected to it to move back and forth in the horizontal direction, thereby driving the vertical force transmission rod 604 fixed thereto and the swing ring 605 connected to the vertical force transmission rod 604 to move back and forth, so as to achieve the swing positioning basket assembly 7 installed inside the swing ring 605 and the culture bag 8 inside the swing positioning basket assembly 7. Several full-spectrum spotlights 606 are installed in the middle and upper part of the vertical force transmission rod 604 to facilitate the observation of the growth status of the culture in the culture bag 8 and serve as a light source for lighting.

[0082] See also Figure 1 and Figure 7 The swing positioning basket assembly 7 includes a positioning basket 701, and a plurality of swing positioning basket ears 702 are provided at the bottom, side and middle of the positioning basket 701. The upper part of the positioning basket 701 is provided with a handle 703 with two ears that pass through two ends and face each other in the horizontal direction to form a handle-shaped hanging rope. The ears 702 at the bottom of the swing positioning basket are connected to the rocker ring with a soft rope and are evenly positioned along the rocker ring, so that the swing positioning basket 7 can accurately transmit the swing of the swing frame assembly. The swing positioning basket handle 703 is hung on the ring welded on the lower side of the inverted cone support bearing 12 with a movable spring ring, and the inverted cone support bearing 12 is fixed on the first support plate 10. The inner ring of the inverted cone support bearing 12 can rotate flexibly with the swing of the swing bag.

[0083] Specifically, the swing positioning basket side ear 702 provides a lateral connection position, which can be connected to the tie rope on the culture bag 8 after the culture bag 8 is placed, so as to reduce the shaking impact of the culture bag 8 inside the swing positioning basket assembly 7; the swing positioning basket assembly 7 is suspended below the culture bag inverted conical support bearing 12 through the positioning basket handle 703, so that the swing positioning basket assembly 7 is suspended inside the telescopic water bath heat insulation barrel 5, without affecting the swing of the culture bag 8 arranged inside the swing positioning basket assembly 7, and ensuring the normal progress of cell mixing.

[0084] Please refer to Figure 1 、 Figure 7 and Figure 8, the culture bag 8 is a disposable sterile bag, including a soft bag body 801, a first paddle shaft and paddle blades 802, a second paddle shaft and paddle blades 803, a double-layer hard bag bottom 804, a bag neck sealing film 805, an annular hard bag neck 806, and a double-layer hard bag shoulder 807; a culture bag sealing cover 808. Inside the inner bag bottom of the double-layer hard bag bottom, rotatable sterile components are installed: a first paddle shaft and paddle blades 802 and a second paddle shaft and paddle blades 803. The soft bag body 801 is sandwiched between the double-layer hard bag shoulders 806 and the double-layer hard bag bottom and is made by bonding / heat-melting process. The upper part of the inner circle of the double-layer hard bag shoulder 807 is fused with the lower part of the annular hard bag neck 806. There is an internal thread on the inner side of the annular hard bag neck 806, and the internal thread is used to screw on the gas-liquid filtration port cover 9. When there is no component 9 screwed on the internal thread of the annular hard bag neck 806, in order to maintain the sealed and sterile state, a component with a threaded connection port - a sterile culture bag sealing cover 808 is screwed on. The rigid gas-liquid filtration port cover 9 that is rotationally and hermetically installed and connected with the circular hard bag neck 806 by a rotating thread is also a disposable sterile component. There are multiple pipeline ports on the gas-liquid filtration port 9, which are used to install the inlet pipe, the exhaust pipe, and the inlet pipe. There are also an inoculation port for inoculation, a sampling port for adding samples, and a gas detection port for detecting the gas components inside the culture bag. All these interfaces are equipped with sterilization filters of corresponding calibers. Each pipeline and ventilation port installed on the gas-liquid filtration port of component 9 is a sterile disposable component. The culture bag 8 is placed in the swing positioning basket assembly 7 and is supported and positioned. The lugs 702 on the bottom side and the side of the swing positioning basket are combined and positioned with the holding rings of the swing frame, which enables the swing of the swing frame to be accurately transmitted to the swing positioning basket assembly 7 and the culture bag 8 therein. During the swinging process of the culture bag 8, the suspended cell culture material inside the culture bag 8 can drive the first paddle shaft and paddle blades 802 and the second paddle shaft and paddle blades 803 to rotate. While ensuring that the suspended cell culture material will not be sheared, it further increases the mixing efficiency of the suspended cell culture material inside the culture bag 8 and prevents cell precipitation. A bag neck sealing film 805 is provided at the top of the culture bag 8, which can cooperate with the sealing film 908 of the gas-liquid filtration port cover and is pressed by a sealer to increase the sterile sealing effect and further ensure the sterile sealed connection between the culture bag 8 and the gas-liquid filtration port cover 9.

[0085] Please refer to Figure 1 , Figure 9 and Figure 10The gas-liquid filter port cover 9 is also provided with an anaerobic mode air inlet 902 for leading out the oxygen-containing culture gas inside the culture bag 8. The anaerobic mode air inlet 902 is provided on the gas-liquid filter port cover 9 as an outlet for leading out the oxygen-containing culture gas, which provides a prerequisite for the subsequent anaerobic cell culture in the culture bag 8 in cooperation with the anaerobic generating component 17; the lower side of the gas-liquid filter port cover 9 is connected to a bag inner tube air inlet long tube 906, and the upper end of the bag inner tube air inlet long tube 906 is connected to the air inlet pipe interface 901 for passing Different types of culture gases are introduced, the lower end of the bag inner tube air inlet long tube 906 extends to the lower side of the culture bag 8, and the lower end of the bag inner tube air inlet long tube 906 is connected to the outlet pipe interface position with a shower head, and the bag inner tube air inlet long tube 906 is connected to the air inlet pipe interface 901 provided on the gas-liquid filter port cover 9, and the gas introduced through the air inlet pipe interface 901 can enter the lower side of the culture bag 8 through the bag inner tube air inlet long tube 906, which can not only increase the mixing efficiency, but also reduce the shear force of gas introduction and reduce the damage of cultured cells. A plurality of outlet pipe interfaces 905 are installed on the gas-liquid filter port 9, the inside of the culture bag is connected to the bag inner tube outlet short tube 907, and the outside of the port cover is connected to the corresponding filter sterilizer.

[0086] See also Figure 1 , Figure 9 , Figure 10 and Figure 11 A gas distribution assembly 13 is installed on the second support plate 11, and the gas distribution assembly 13 includes a plurality of gas distribution inlet pipes 1301 connected to the inlet pipe interface 901, a gas distribution exhaust pipe 1306 connected to the outlet pipe interface 905, and a gas distribution control host 1305 for controlling the opening and closing of corresponding pipelines; different gas distribution inlet pipes 1301 are connected to different types of gas sources, which can be external flowing air or other types of gases released from a cylinder at a certain pressure and flow rate. A gas distribution sterilization filter 1302 is provided at one end of the gas distribution inlet pipe 1301 away from the gas inlet pipe interface 901. The gas distribution sterilization filter 1302 is provided at the end of the gas distribution inlet pipe 1301 to filter the gas distribution to ensure the cleanliness of the gas distribution and avoid the problem of contamination of the suspended cell culture inside the culture bag 8 caused by other substances or bacteria; the pipeline of the gas distribution inlet pipe 1301 is connected with a gas distribution valve 1303 and a flow meter 1304, and a pressure gauge to increase the precise gas distribution function in the cell culture process through the gas distribution component 13, so that the required gas can be passed through the gas distribution inlet pipe 1301 to the inside of the culture bag 8 as a culture container, and the flow rate is measured by the flow meter 1304, and the data is transmitted back to the central control system, and the deviation is adjusted by adjusting the gas distribution valve 1303 to ensure the accuracy of the corresponding gas distribution amount; the pipeline of the gas distribution exhaust pipe gas 1306 is connected with a control valve.

[0087] See also Figure 1 , Figure 9 , Figure 10 and Figure 12, a gas detection component 14 is installed on the second support plate 11. The gas detection component 14 includes a gas detection host 1401, a gas detection probe tube 1402, and a gas detection probe group 1403. The upper part of the gas detection probe tube 1402 is connected to the gas detection host 1401, and the lower part of the gas detection host 1401 is installed with the gas detection probe group 1403. The lower part of the gas detection probe tube 1402 is connected to the gas detection port valve 908. The gas detection component 14 is used to monitor the gas concentration inside the culture bag 8 (cell culture container). The gas detection probe group 1403 can monitor the concentration of the corresponding gas components at the gas detection port 904 connected to the gas detection probe tube 1402. This concentration data is equivalent to the concentration of the culture gas components in the gas phase space inside the culture bag 8. The gas detection component 14 can transmit the concentration data of the culture gas components back to the central control module. The central control module analyzes and evaluates this data and issues an instruction to change or maintain the parameters of the original gas distribution module. The central control module adjusts the gas component environment inside the culture bag 8 to the optimal culture gas environment for suspension cell culture by controlling the concentration, flow rate, and flow of the gas delivered by the gas distribution component.

[0088] Among them, the gas detection probe group 1403 includes a carbon dioxide detection probe commonly used in biological culture, a commonly used oxygen detection probe, and the third probe is a variable probe for detecting other types of gases, which is selected according to the newly added culture gas components. The device can be set to a positive pressure state during cultivation. The positive pressure state makes the entire device not easily contaminated during the cultivation process. When other new culture gas components are added and the system has not temporarily replaced the corresponding new component detection probe to the variable probe position, the simple procedure for temporarily detecting the culture gas type without the corresponding type of detection probe is as follows: when the device is in a positive pressure state, collect the exhaust gas discharged from the gas distribution exhaust pipe 1306 with a standard gas collection bag, send the collected gas sample for inspection, and detect the gas in the gas sample collection bag with the corresponding detection equipment, so as to quickly detect the concentration of other types of gases in the culture gas without replacing the gas detection probe.

[0089] Please refer to Figure 1 , Figure 9 , Figure 10 and 13, an anaerobic generation assembly 17 is installed on the installation position 112 of the second support plate 11. The anaerobic generation assembly 17 includes an anaerobic generation gas pipe 1701 communicated with the anaerobic mode air inlet 902. The other end of the anaerobic generation gas pipe 1701 extends into the interior of the glass cover 1705 to uniformly and slowly release the gas in the culture bag 8 into the glass cover 1705. An electronic ignition burner 1703 is arranged inside the glass cover 1705. The electronic ignition burner 1703 ignites the natural gas input into the glass cover 1705 through the gas delivery pipe 1704 to consume the oxygen in the culture bag 8. An anaerobic generation flame retarder 1702 located outside the combustion device and an explosion-proof and flame-retardant device 1708 close to the combustion device are installed on the pipeline of the anaerobic generation gas pipe 1701; an anaerobic exhaust pipe 1706 communicated with the interior of the glass cover 1705 is connected to the top of the glass cover 1705. An anaerobic exhaust valve 1707 is connected to the pipeline of the anaerobic exhaust pipe 1706. By setting the anaerobic generation assembly 17, the oxygen inside the culture bag 8 is consumed, creating the conditions for anaerobic culture, increasing the types of cells and microorganisms that can be enriched by the anaerobic generation assembly, and increasing the practicality of the device; the electronic ignition burner 1703 inside the glass cover 1705 ignites the gas supplied by the gas delivery pipe 1704 to burn and consume the oxygen introduced into the glass cover 1705 through the anaerobic generation gas pipe 1701 inside the culture bag 8, providing anaerobic culture conditions and realizing anaerobic mode culture operation to make the interior of the culture bag 8 in an oxygen-deficient state; setting the anaerobic exhaust pipe 1706 to discharge other gases generated after the oxygen is burned to avoid affecting the culture environment inside the culture bag 8 and also avoid the problem of the glass cover 1705 bursting due to excessive internal pressure. An explosion-proof and flame-retardant device 1708 is set to ensure the safety of the device, and an anaerobic exhaust protector 1709 is set to prevent external organisms from drilling into the device through the anaerobic exhaust pipe 1706;

[0090] Please refer to Figure 1 , Figure 14 and Figure 15 , a temperature control assembly 16 is installed on the external temperature control device installation position 1103 on the second support plate 11. The temperature control assembly 16 includes a temperature control main unit 1601. The temperature control main unit 1601 is installed at the top end of the temperature control device protection cover 1602, and the bottom end of the temperature control device protection cover 1602 extends downward into the interior of the telescopic water bath heat insulation barrel 5. A stirring paddle 1603, a temperature measuring probe 1604 and a temperature control pipe 1605 that extend into the interior of the temperature control device protection cover 1602 are connected to the lower end of the temperature control main unit 1601.

[0091] Specifically, a constant-temperature component 16 is provided to cooperate with the telescopic water bath heat-insulating barrel 5 to enhance the constant-effect of the water bath temperature inside the telescopic water bath heat-insulating barrel 5. The temperature-measuring probe 1604 in the constant-temperature component 16 can monitor the water bath temperature inside the telescopic water bath heat-insulating barrel 5 in real time. When the temperature inside the telescopic water bath heat-insulating barrel 5 drops below the optimal water bath temperature range, the water inside the telescopic water bath heat-insulating barrel 5 is heated through the constant-temperature pipe 1605 in the constant-temperature component 16, and the stirring paddle 1603 is used to keep the water temperature distribution inside the telescopic water bath heat-insulating barrel 5 uniform, so as to ensure that the water bath temperature inside the telescopic water bath heat-insulating barrel 5 is within the optimal range.

[0092] Further, a control system mounting plate 18 for mounting each control module of the control system is installed on the second support plate 11. The control modules of the control system include a central control module 18L for setting, controlling, and coordinating the whole machine culture mode, a mixing control module 6L for controlling the swing frame assembly 6 to swing, a gas distribution quantitative delivery module 13L for controlling the gas distribution assembly 13 to quantitatively deliver specified gases, a gas component monitoring module 14L for cooperating with the gas detection component 14 to monitor gas components, an anaerobic control module 15L for controlling the anaerobic generation component 17 to consume oxygen by combustion, and a culture temperature control module 16L for controlling the constant-temperature component 16 to keep the water bath temperature inside the telescopic water bath heat-insulating barrel 5 constant.

[0093] Among them, the central control module is used to set and coordinate the culture mode, control other modules according to different modes, analyze and evaluate the data transmitted back by other modules, and adjust system operation parameters such as the temperature of cell culture, the flow rate and concentration of gas distribution, and the mixing rate, so as to meet the culture requirements of different types of cells. The mixing control module can control the swing frame assembly 6 to start, stop, and change the swing frequency to quickly achieve the purpose of gently mixing the cells inside the culture bag 8; the gas distribution quantitative delivery module can control the gas distribution assembly 13 to accurately deliver different gases quantitatively; the gas monitoring module can control the gas detection component 14 to detect the concentration and content of each gas component inside the culture container and transmit the detected data back to the central control module for the central control system to timely regulate the gas distribution assembly 13; the anaerobic control module can control the anaerobic generation component 17 to start when anaerobic cell culture is required inside the culture container, so as to consume the oxygen inside the culture container to create an anaerobic culture environment; the culture temperature control module can control the constant-temperature component 16 to make the water temperature inside the telescopic water bath heat-insulating barrel 5 within the suitable culture temperature range and ensure the constancy of the water bath temperature.

[0094] The suspension cell culture device can be used for culturing suspension cells. During specific implementation, before culturing suspension cells, the installation of the device is carried out first. First, place the base 1, and then place the heat insulation plate 104 on the base 1. According to the size of the position space and the number of suspension cells to be cultured, a first vertical sleeve 3 is successively sleeved on the first fixed tube 103 fixed on the top of the base 1 to form an outer mounting frame, and a second vertical sleeve 2 is successively sleeved on the second fixed tube 102 fixed on the top of the base 1 to form an inner mounting frame. Subsequently, place the telescopic water bath heat insulation barrel 5 inside the inner mounting frame, and fix it to the inner mounting frame through the aluminum alloy barrel clamp and barrel hoop assembly 15 connected to the upper and lower ends of the telescopic water bath heat insulation barrel 5. Then, install a swing assembly extending into the telescopic water bath heat insulation barrel 5 on the outer mounting frame, install a swing positioning basket assembly 7 on the frame body of the swing assembly extending into the telescopic water bath heat insulation barrel 5, and finally install a first support plate 10 and a second support plate 11 on the top of the outer mounting frame. Install a gas-liquid filtration port cover on the first support plate 10, and install a gas distribution assembly 13, a gas detection assembly 14, an anaerobic generation assembly 17, and a constant temperature assembly 16 on the second support plate 11 to complete the installation of the culture device;

[0095] When culturing suspension cells, in a laminar flow hood / clean room, place an empty sterile culture bag 8 inside the swing positioning basket assembly 7, inject the culture medium and necessary chemical substances required for cell culture into the culture bag 8, inoculate the cells to be cultured, and then screw the bag neck annular hard bag neck 806 of the culture bag 8 tightly with the gas-liquid filtration port cover 9. Align the outer ring on the upper side of the annular hard bag neck 806, that is, the bag neck sealing film 805 on the side of the annular hard bag neck 806, with the gas-liquid filtration port cover sealing film 908, and press and seal the docking overlapping area with a sealer.

[0096] Suspend the swing positioning basket assembly 7 with the sterile culture bag 8 placed therein on the hook at the bottom of the tapered bearing on the first support plate 10 by means of the handle 703, and place this swing positioning basket assembly 7 inside the telescopic water bath heat insulation barrel 5. Adjust the height of the telescopic water bath heat insulation barrel according to the height of the sterile culture bag 8, and correspondingly adjust the number of sleeves of the first vertical sleeve 3 and the second vertical sleeve 2, so that the actual height of the inner and outer mounting frames is suitable for the actual cell culture requirements. Connect the telescopic water bath heat insulation barrel 5 and the second vertical sleeve 2 with the spring buckle fixing ring 201. Connect the sterile gas pipeline and the gas filtration and sterilization device, and turn on the gas distribution system to set the culture gas pressure and concentration. Turn on the swing frame assembly 6 and the position of the external temperature control assembly 16. Inject distilled water at an appropriate temperature corresponding to the type of cultured cells into the telescopic water bath heat insulation barrel 5. Turn on the central control module and other control modules, and set the central control module. Install and fit two semi-circular foam plastic heat insulation covers 19 separately from the outer side of the gas-liquid filtration port cover 9. Place the foam plastic heat insulation cover 19 on and fit it to the top of the telescopic water bath heat insulation barrel, and select a suitable one from the micro heat insulation covers 1901, 1902, 1903 to cover the non-covered area of the swing frame. Thus, the preparation work before suspension cell culture is completed.

[0097] During suspension cell culture, the culture gas / domestication gas of the required type and concentration can be accurately and quantitatively sent into the interior of the culture bag 8 through the gas distribution assembly 13. At the same time, the swing frame assembly 6 drives the culture bag 8 to swing, so that the suspension cell culture in the culture bag is fully mixed. At the same time, the gas detection assembly 14 can be used to detect and feedback the gas concentration inside the culture bag 8 in real time to ensure that the culture environment inside the culture bag 8 is in the optimal culture environment; during the suspension cell culture process, if it is necessary to culture cells under anaerobic conditions, the anaerobic generation assembly 17 can consume all the oxygen inside the culture bag 8 to provide the corresponding anaerobic culture environment; during the suspension cell culture process, the water bath temperature inside the telescopic water bath heat insulation barrel 5 will inevitably dissipate. At the same time when this problem occurs, the constant temperature assembly 16 will be started, and the dissipated heat will be compensated by heating with the constant temperature tube 1605, so as to ensure that the water bath temperature inside the telescopic water bath heat insulation barrel 5 remains within the floating range of the optimal cell culture environment temperature, thereby increasing the cell culture efficiency.

[0098] Specifically for the three major categories of cell culture:

[0099] (1) The advantages of the device for microbial culture are that it can provide anaerobic and aerobic culture modes, which are suitable for the anaerobic and aerobic culture of microorganisms for waste gas treatment and other types of microorganisms. When culturing microorganisms for industrial waste gas treatment, domesticated gas with controlled concentration needs to be introduced. It also meets the cultivation requirements of other types of microorganisms. For example, when producing anaerobic bacteria vaccine products by anaerobic culture, strict requirements are needed for cleanliness, culture gas, temperature, etc. When used for anaerobic culture, it is necessary to continuously maintain the concentration of inert gases such as nitrogen and carbon dioxide.

[0100] (2) The device is used for in vitro suspension culture of plant cells, such as in the process of producing drugs, and strict aseptic operation procedures need to be followed. First, in a sterile laminar flow hood, the culture medium for plant cell suspension culture, plant growth hormone - plant hormone, and other necessary reagents need to be added to the culture bag 8 in an aseptic operation process. And digested and dispersed plant cells need to be added; then, turn on the light, connect the pipeline and fixing device, and start the temperature control device of the system to the culture temperature. The device can avoid shear damage to cells caused by the stirring force in plant cell suspension culture and meet the light requirements of plant cell suspension culture.

[0101] (3) The device is used for animal cell culture. Strict aseptic operation procedures need to be followed. First, in a sterile laminar flow hood, serum for animal cell culture, digested and dispersed cells with reagents such as trypsin, and other reagents need to be added to the culture bag 8 in an aseptic operation process. When the culture medium has no buffer system, sterile buffer reagents such as sodium bicarbonate and HEPES also need to be added to the culture medium. If the carbon dioxide concentration in the gas space without liquid in the culture bag is set at 5%, the addition amount of sodium bicarbonate in the culture medium should be 1.97 g / L; if the carbon dioxide concentration in the gas space without liquid in the culture bag is set at 10%, the addition amount of sodium bicarbonate should be 3.95 g / L. Then, connect the pipeline and fixing device, and start the temperature control device of the system to the culture temperature; when the depth of the culture medium exceeds 5 mm, the rocking and mixing device needs to be started to achieve the effect of agitating the culture medium; when the depth of the culture medium exceeds 10 cm, the gas supply device also needs to be started to deeply introduce carbon dioxide and air with precisely controlled concentrations to ensure sufficient gas exchange and accurate carbon dioxide culture gas concentration. Cooperating with the buffer system in the culture medium (such as sodium bicarbonate and HEPES), the pH of the culture medium can be maintained. In addition, the above embodiments only illustrate the principles and effects of the present invention by way of example, and are not used to limit the present invention. Any person familiar with this technology can modify or change the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes completed by those with ordinary knowledge in the technical field without departing from the spirit and technical idea disclosed by the present invention should still be covered by the claims of the present invention.

[0102] In summary, a suspension cell culture device of the present invention has the following beneficial effects:

[0103] (1) The use of a disposable sterile culture bag 8 replaces the fermenter to provide a sterile culture space for suspension cells, saving the cost of expensive fermenters. Different cells can be cultured through the disposable sterile culture bag 8, eliminating the process of fermenter sterilization required before each suspension cell culture, avoiding the problem of cross-contamination caused by incomplete sterilization of the culture container, and saving energy, manpower, and time, thereby improving production efficiency.

[0104] (2) An external swing frame assembly 6 and an external constant temperature assembly 16 that do not directly contact the culture are provided, effectively reducing the damage rate of cell culture and the probability of contamination. The external swing frame assembly 6 drives the culture bag 8 to swing, promoting the dispersion of cells inside the culture bag 8. Compared with setting up a stirring mechanism for mixing, it will not cause the problem of cell shear damage due to the setting of the stirring structure, and can effectively reduce the damage rate of cell culture; the outer mounting frame and the inner mounting frame that form the basic support of the device have the functions of simple disassembly and height adjustment, with high flexibility in use, and can adapt to the needs of culture bags of different volumes after adjustment to match the volumes of different telescopic water bath heat insulation barrels 5 and cooperate with the heights of different telescopic water bath heat insulation barrels 5. By adjusting the height of the mounting frame, it can adapt to the needs of cell culture in different capacities and different space environments, and has a low manufacturing cost, meeting the needs of the general public.

[0105] (3) A gas distribution component 13 is provided. Under the setting of the control system, specific concentrations of combined culture gases can be supplied to the culture bag according to different parameters such as the molecular weight and air pressure of different types of gases. To achieve the supply of different types of culture gases, for example: supplying a culture gas containing specific concentrations of oxygen and carbon dioxide for animal cell culture. For example: supplying a gas containing sulfide for screening, domesticating, and culturing sulfur-metabolizing microorganisms that can metabolize sulfide, etc., and can accurately supply the required culture gas quantitatively, enriching the types of cells that can be cultured by the culture device. The gas will be sterilized by the sterile filter installed on the pipeline before entering the culture bag 8, and can quickly adjust the gas environment inside the culture bag 8 to the most suitable state for the required cell culture, thereby increasing the efficiency of cell culture; a full-spectrum spotlight 606 is provided on the swing frame 6 to meet the culture of cells that require light.

[0106] (4) A gas detection component 14 is provided. Besides being able to cooperate with the gas distribution component 13 to achieve precise adjustment of the gas environment inside the culture bag 8, it can also timely feedback the gas state inside the cell culture bag, display the concentration of each culture gas component inside the culture bag in a sterile culture environment, so as to facilitate the central control system to timely adjust the flow rate, flow volume and concentration of the supplied culture gas. It is suitable to further increase the adjustment efficiency of adapting to environmental conditions during subsequent cell culture, thereby increasing the cell culture efficiency.

[0107] (5) An anaerobic generation component 17 is provided. The anaerobic generation component 17 can consume the oxygen inside the culture bag 8, making the inside of the culture bag 8 in an anaerobic environment, so that the culture operation of anaerobic cells can be carried out, increasing the range of cell types that the device can culture; a constant temperature component 16 is provided, which can make up for the loss of the water bath temperature inside the thermostatic water bath heat insulation barrel 5 through the constant temperature component 16, so as to maintain the water temperature inside the thermostatic water bath heat insulation barrel 5, and further maintain the constant culture temperature of the cultured cells, increasing the cell culture efficiency.

[0108] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations fall within the scope of the claims of the present invention and their equivalent technologies, the present invention also intends to include these modifications and variations.

Claims

1. A multifunctional general-purpose suspension cell culture device, characterized in that It includes a circular base, at the top of which there is an outer mounting frame and an inner mounting frame inside the outer mounting frame, and inside the inner mounting frame there is a telescopic water bath heat insulation barrel; On the outer mounting frame, there is a swing assembly, which is connected to a swing positioning basket arranged inside the telescopic water bath heat insulation barrel. Through this, the swing assembly drives the swing positioning basket to swing inside the telescopic water bath heat insulation barrel; Inside the swing positioning basket, there is a culture bag, the annular hard bag neck at the top of the culture bag is connected to a gas-liquid filtration port cover, and the gas-liquid filtration port cover is installed on a circular first support plate; The first support plate is installed on the outer mounting frame, and above the first support plate and at the top of the outer mounting frame, there is a circular second support plate parallel to the first support plate; On the gas-liquid filtration port cover, there are 3 air inlet pipe interfaces for connecting the air inlet pipeline, 3 air outlet pipe interfaces for connecting the air outlet pipeline, an inoculation port for inoculation, a sample addition port for adding samples, and a gas detection port for detecting the gas components inside the culture bag. All these interfaces are equipped with sterilization filters of corresponding calibers; In addition, at the top of the gas detection port, there is another detection port valve installed, and the detection port valve is only opened when detecting gas components.

2. The multifunctional universal suspension cell culture device according to claim 1, wherein, At the bottom end of the circular base, lifting rollers are evenly spaced along the circumference, and at the top of the base, there is a circular heat insulation plate, and on the top of the heat insulation plate, there is a groove for installing the bottom end of the telescopic water bath heat insulation barrel.

3. The multifunctional universal suspension cell culture device according to claim 1, characterized in that, The outer mounting frame includes outer columns evenly spaced along the circumference at the top of the circular base. The outer columns include first vertical sleeves sleeved on each other from bottom to top. The lower end of the lowermost first vertical sleeve in the outer column is sleeved on the upper end of a first fixed pipe, and the first fixed pipe is vertically fixed on the base. When only one first vertical sleeve is sleeved on the upper end of the first fixed pipe, the height reached can meet the requirements of the minimum culture capacity; On any part of the first vertical sleeve near the upper and lower ends, there are centripetal trustees installed; The centripetal trustee at the upper end of the uppermost first vertical sleeve of the outer column is used to install the first support plate, and the centripetal trustee at the lower end of the uppermost first vertical sleeve of the outer column is used to install the bottom surface of the second support plate.

4. The multifunctional and general-purpose suspension cell culture device according to claim 1, characterized in that, The inner mounting frame includes inner columns evenly spaced along the circumference at the top of the circular base. The inner columns include second vertical sleeves sleeved on each other from bottom to top. The lower end of the lowermost second vertical sleeve in the inner column is sleeved on the upper end of a second fixed pipe, and the second fixed pipe is vertically fixed on the base; By sleeving different numbers of second vertical sleeves on the upper end of the second fixed pipe, inner columns of different heights can be obtained; On the side of any second vertical sleeve, there is a centripetal fixing ring installed, and the centripetal fixing ring is used to connect and position the positioning bolts and barrel hoops of the telescopic water bath heat insulation barrel.

5. The multifunctional and general-purpose suspension cell culture device according to claim 1, wherein, The telescopic water bath heat-insulating bucket is provided with two layers of foldable barrel walls. The serrated folding areas of the barrel walls are divided into large tooth areas and small tooth areas. Among them, U-shaped positioning bolts are evenly spaced in the small tooth area of the outer barrel wall. A heat-insulating interlayer is formed between the two layers of barrel walls. An inflation port communicating with the inside of the heat-insulating interlayer is opened at the top end of the telescopic water bath heat-insulating bucket. The inner ring of the inflation port is engraved with threads and is connected to a sealing plug with threads engraved on the outer ring. Aluminum alloy barrel clamps and barrel hoop assemblies are installed on the upper barrel edge of the telescopic water bath heat-insulating bucket and the outer tooth area of the barrel wall. The inner rings of the aluminum alloy barrel clamps and barrel hoop assemblies are provided with serrated cavities that closely fit the outer tooth area of the outer wall of the telescopic water bath barrel. Positioning holes are evenly spaced centripetally on the side of the aluminum alloy barrel clamps and barrel hoop assemblies, and the positioning holes cooperate with the U-shaped positioning bolts in the small tooth area of the outer barrel wall. Connecting rings are evenly spaced circumferentially on the outer ring of the aluminum alloy barrel clamps and barrel hoop assemblies, and the connecting rings are connected in cooperation with the fixed rings at the corresponding positions on the inner mounting frame.

6. The multifunctional and general-purpose suspension cell culture device according to claim 5, characterized in that The swinging assembly includes a swinging positioning basket assembly and a swinging frame assembly. The swinging frame assembly includes a vertical force transmission rod, a full-spectrum spotlight, a horizontal force transmission rod, a swing arm rod, a motor, and a swing ring composed of two semi-circles that can hold and connect the swinging positioning basket. The side of the swing ring is connected to the lower end of the vertical force transmission rod vertically arranged inside the telescopic water bath heat-insulating bucket. Several full-spectrum spotlights are installed in the middle of the vertical force transmission rod as the light sources for lighting. The upper end of the vertical force transmission rod is fixedly connected to one end of the horizontal force transmission rod. The other end of the horizontal force transmission rod is connected to the upper end of the swing arm rod. The lower end of the swing arm rod is fixedly connected to the swinging output shaft of the motor, and the motor is fixed on the outer mounting frame.

7. The multifunctional and general-purpose suspension cell culture device according to claim 6, characterized in that, The swinging positioning basket assembly includes a positioning basket; a plurality of lugs evenly distributed on the outside of the positioning basket; a lifting rope that passes through two horizontally opposite lugs on the upper side of the positioning basket to form a handle shape and is used to hang the positioning basket, which is called the positioning basket handle; a soft hanging rope used to position the swinging positioning basket on the swing ring. The lugs evenly arranged on the outer side of the bottom end of the positioning basket are side lugs, and the lugs evenly arranged on the outer side of the top end of the positioning basket are upper lugs. The lifting rope serving as the positioning basket handle has both ends fixed to two horizontally opposite upper lugs, and the middle of the lifting rope is suspended on the hanging ring on the lower side of the inverted conical bearing. The inverted conical bearing is fixed on the first support plate.

8. The multifunctional universal suspension cell culture device according to claim 1, wherein, The culture bag comprises an annular hard bag neck, a bag neck sealing film, a double-layer hard bag shoulder, a soft bag body, a double-layer hard bag bottom, a first paddle shaft and paddle blade, and a second paddle shaft and paddle blade; the culture bag is a disposable sterile culture bag, and the inner layer and outer layer of the double-layer hard bag bottom and the double-layer hard bag shoulder sandwich the corresponding parts of the soft bag body and are fixedly connected and combined; the inner layer of the double-layer hard bag bottom is connected with the first paddle shaft and paddle blade and the second paddle shaft and paddle blade; the annular hard bag neck, double-layer hard bag shoulder, double-layer hard bag bottom, paddle shaft, and paddle blade are made of a material with a certain thickness, rigidity, and hardness, and the shape and performance of the material are stable in the temperature range of 60-90 degrees Celsius, and they are transparent materials; this makes the sterile culture bag sturdy and durable, and allows the paddle shaft and paddle blade at the bottom of the sterile culture bag to swing flexibly; The upper part of the sterile culture bag is a hard annular hard bag neck, and the annular hard bag neck and the gas-liquid filter port cover are screwed together through the threads engraved on the port to achieve a sealed connection; the gas-liquid filter port cover is installed on the inner ring of the inverted cone bearing; a sealing film is provided on the outer side of the lower part of the gas-liquid filter port cover, and a bag neck sealing film is provided on the upper outer ring of the annular hard bag neck of the sterile culture bag, and the two sealing films are butted together and sealed with a sealer.

9. The multifunctional universal suspension cell culture device according to claim 8, wherein: The gas-liquid filter port cover is also provided with an air inlet for drawing out oxygen from the culture bag; The lower side of the gas-liquid filter port cover is connected to a sterile long air intake tube, the upper end of the long air intake tube is connected to the air intake pipe interface, the lower end of the long air intake tube extends to the lower side of the culture bag, and the lower port of the long air intake tube is connected to a sterile shower head.

10. The multifunctional universal suspension cell culture device according to claim 5, characterized in that: At the top of the telescopic water bath insulation barrel, surrounding the outer part of the gas-liquid filter port cover, there are two semicircular foam insulation plastic insulation covers, which can be used to reduce the temperature fluctuation in the water bath barrel. On the surface of the insulation cover, there are narrow and long candied haws-shaped three-layer openings in a stepped manner. The function of this opening is to provide space for the swing arm of the swing frame to swing freely. In the non-touching area of the swing arm, a micro foam plastic round cover can be covered for insulation.

11. The multifunctional universal suspension cell culture device according to claim 1, wherein: The second support plate is provided with an air distribution assembly, which includes a plurality of air distribution pipes connected to the air inlet pipe interface, an air distribution exhaust pipe connected to the air outlet pipe interface, and an air distribution host for controlling the opening and closing of corresponding pipes; An air distribution sterilization filter is arranged at one end of the air distribution pipe away from the air inlet pipe interface, and an air distribution valve and a flow meter are connected to the pipeline of the air distribution pipe; a control valve is connected to the pipeline of the air distribution exhaust pipe.

12. The multifunctional universal suspension cell culture device according to claim 11, characterized in that: A gas detection component is installed on the second support plate. The gas detection component includes a probe tube connected to the gas detection port. The top of the probe tube is connected to a gas detection host. The lower end of the gas detection host is connected to a gas detection probe extending into the probe tube.

13. The multifunctional universal suspension cell culture device according to claim 12, wherein: An anaerobic generating assembly is installed on the second supporting plate, and the anaerobic generating assembly includes an anaerobic air duct connected to the air duct port, and the other end of the anaerobic air duct extends to the inside of the glass cover, and an electronic igniter is arranged inside the glass cover, and the electronic igniter is connected to an external gas source through a gas delivery pipe; An anaerobic filter is installed on the anaerobic air duct; An anaerobic gas outlet pipe communicating with the interior of the glass cover is connected to the top of the glass cover, and an anaerobic gas outlet valve is connected to the pipeline of the anaerobic gas outlet pipe.

14. The multifunctional universal suspension cell culture device according to claim 1, wherein: A constant temperature assembly is installed on the second support plate. The constant temperature assembly includes a constant temperature controller installed at the top of a constant temperature heating protection cover, and the bottom end of the constant temperature heating protection cover extends downward into the interior of the telescopic water bath heat insulation barrel. A stirring paddle, a temperature measuring probe, and a heating pipe extending into the constant temperature heating protection cover are connected to the lower end of the constant temperature controller.

15. The multifunctional universal suspension cell culture device according to claim 14, characterized in that: A control system installation plate for installing each control module of the control system is installed on the second support plate. The control system includes a central control module for setting the overall machine culture mode, a mixing control module for controlling the swing assembly to swing, a gas distribution quantitative delivery module for controlling the gas distribution assembly to quantitatively deliver specified gases, a gas component monitoring module for cooperating with the gas detection assembly to monitor gas components, an anaerobic control module for controlling the anaerobic generation assembly to burn oxygen, and a culture temperature control module for controlling the constant temperature assembly to keep the water bath temperature inside the telescopic water bath heat insulation barrel constant.

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