Immune cell cryopreservation device

Abstract

The utility model relates to the technical field of cell cryopreservation devices, in particular to an immune cell cryopreservation device. According to the technical scheme, the cryopreservation box comprises a cryopreservation box body, a sealing cover is arranged above the cryopreservation box body, a plurality of cryopreservation containing cavities are formed in the cryopreservation box body, a cryopreservation tube adapter is arranged in each cryopreservation containing cavity, cryopreservation tube grooves are formed in the cryopreservation tube adapters, cryopreservation tubes are arranged in the cryopreservation tube grooves, and the cryopreservation tubes are arranged in the cryopreservation tube grooves. A pipe cap is arranged at one end of the cryopreservation pipe, a limiting seat is arranged in the cryopreservation box, a limiting pipe is arranged below the sealing cover, a cover lug is arranged on the other side of the sealing cover, and a connecting column is arranged below the cryopreservation box. According to the immune cell cryopreservation device, immune cells can be stably placed, meanwhile, the low-temperature state of the immune cells in the cryopreservation state can be guaranteed, therefore, the vigor state of the immune cells is guaranteed, meanwhile, multiple sets of immune cell cryopreservation devices can be stacked, occupied space is reduced, and operators can take and place the immune cells greatly conveniently.

Description

Technical Field

[0001] The utility model relates to the technical field of cell cryopreservation devices, in particular to an immune cell cryopreservation device. Background Technique

[0002] Immune cell cryopreservation is to freeze and store immune cells at a very low temperature, usually below -196°C, to extend their lifespan and ensure their future availability. This is an important technique in various applications, including the treatment of cancer, autoimmune diseases, and infectious diseases. Immune cell cryopreservation is an important research field in biotechnology, clinical medicine, and drug research and development. The process of immune cell cryopreservation begins with separating and purifying immune cells, culturing them in an appropriate medium to make them grow healthily and function actively, then placing them in a specially designed freezing container, using rapid freezing technology to freeze them to near the freezing point, and then putting the container into a liquid nitrogen tank. The temperature of liquid nitrogen is about -196°C, where immune cells are stored to extend their lifespan. However, most of the existing freezing containers for placing immune cells have complex structures, which are not convenient for operators to take and place immune cells, and the freezing containers for cryopreserving immune cells cannot be stacked when placed in a low-temperature environment of liquid nitrogen, which occupies a lot of space. Therefore, we specifically propose an immune cell cryopreservation device. Content of the Utility Model

[0003] The purpose of the utility model is to propose an immune cell cryopreservation device for the problems existing in the background technique.

[0004] The technical solution of the utility model: An immune cell cryopreservation device includes a cryopreservation box, a sealing cover is arranged above the cryopreservation box, multiple cryopreservation placement cavities are opened in the cryopreservation box, a cryopreservation tube adapter is arranged in each cryopreservation placement cavity, a cryopreservation tube slot is opened on the cryopreservation tube adapter, a cryopreservation tube is arranged in the cryopreservation tube slot, a tube cap is arranged at one end of the cryopreservation tube, a limiting seat is arranged in the cryopreservation box, a limiting tube is arranged below the sealing cover, a cover ear is arranged on the other side of the sealing cover, and a connecting column is arranged below the cryopreservation box.

[0005] Preferably, the inner cavity of the cryopreservation box is concave, a sealing ring groove is opened on the cryopreservation box, and the edge position of the sealing cover is correspondingly installed with the sealing ring groove.

[0006] Preferably, the installation end of the cryopreservation tube adapter is correspondingly installed with the inner cavity of the cryopreservation placement cavity, and the outer periphery of the cryopreservation tube adapter is mutually attached to the inner wall of the cryopreservation placement cavity.

[0007] Preferably, the outer periphery of the cryopreservation tube is correspondingly installed with the inner wall of the cryopreservation tube slot, the top end of the cryopreservation tube is set in a threaded shape, and the tube cap is correspondingly installed with the top end of the cryopreservation tube.

[0008] Preferably, the mounting end of the limit seat is correspondingly installed at the middle position of the inner cavity of the cryopreservation box, and a limit opening is provided above the limit seat.

[0009] Preferably, the mounting end of the limit tube is correspondingly installed at the middle position of one side of the sealing cover. A positioning column is arranged inside the limit tube, the outer periphery of the positioning column is in mutual fit with the inner wall of the limit opening, and the outer periphery of the limit seat is in mutual fit with the inner wall of the limit tube.

[0010] Preferably, one side of the cover ear is correspondingly installed with one side of the sealing cover, and the outer periphery of the cover ear is arranged in a wavy shape.

[0011] Preferably, the mounting end of the connecting column is correspondingly installed at one side below the cryopreservation box. The top end of the connecting column is arranged in a polygonal shape. A connecting hole is provided above the cover ear, and the connecting column and the connecting hole are mutually adapted.

[0012] Compared with the prior art, the utility model has the following beneficial technical effects:

[0013] The overall structure of the utility model is simple and the operation is convenient. It can stably place immune cells, and at the same time can ensure the low-temperature state of immune cells in the cryopreserved state, thereby ensuring the viability state of immune cells. At the same time, multiple sets of this device can be stacked to reduce space occupation, and it greatly facilitates the operator's operation of taking and placing immune cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic diagram of the overall structure of the utility model;

[0015] Figure 2 is a schematic diagram of the structure in a separated state of the cryopreservation box and the sealing cover in the utility model;

[0016] Figure 3 is Figure 2 a schematic diagram of another perspective structure of

[0017] Figure 4 is a schematic diagram of a partial area structure of the cryopreservation box in the utility model;

[0018] Figure 5 is a schematic diagram of a partial area structure of the sealing cover in the utility model;

[0019] Figure 6 is an exploded view of the cryopreservation tube adapter and the cryopreservation tube in the utility model.

[0020] Reference numerals: 1, cryopreservation box; 2, sealing cover; 3, cryopreservation placement cavity; 4, cryopreservation tube adapter; 5, cryopreservation tube slot; 6, cryopreservation tube; 7, tube cap; 8, limiting seat; 9, limiting tube; 10, cover ear; 11, sealing ring groove; 12, limiting port; 13, connecting column; 14, connecting hole; 15, positioning column. Detailed implementation mode

[0021] The technical solutions of the present utility model will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0022] Embodiment

[0023] As Figures 1-6 shown, an immune cell cryopreservation device proposed by the present utility model includes a cryopreservation box 1, a sealing cover 2 is arranged above the cryopreservation box 1, the inner cavity of the cryopreservation box 1 is concave, and the concave cryopreservation box 1 can better stably place immune cells. A sealing ring groove 11 is opened on the cryopreservation box 1, and the edge position of the sealing cover 2 is correspondingly installed with the sealing ring groove 11. When the sealing cover 2 and the cryopreservation box 1 are in a corresponding installation state, the edge position of the sealing cover 2 is closely attached to the inner wall of the sealing ring groove 11, so that the cryopreservation box 1 and the sealing cover 2 cooperate with each other to form a sealed state in the inner cavity of the cryopreservation box 1. A plurality of cryopreservation placement cavities 3 are opened in the cryopreservation box 1, and the cryopreservation placement cavities 3 are arranged in an inclined shape. A plurality of cryopreservation placement cavities 3 are arranged in an annular inclined shape. Liquid nitrogen valves are arranged on the bottom inner walls of the cryopreservation placement cavities 3. A cryopreservation tube adapter 4 is arranged in each cryopreservation placement cavity 3. The installation end of the cryopreservation tube adapter 4 is correspondingly installed with the inner cavity of the cryopreservation placement cavity 3, and the outer periphery of the cryopreservation tube adapter 4 is in mutual fit with the inner wall of the cryopreservation placement cavity 3. The inclined arrangement of the cryopreservation placement cavity 3 can make the cryopreservation tube adapter 4 more stable in the placement state, and at the same time can better keep the cryopreservation tube adapter 4 at a set temperature. And liquid nitrogen can be injected into the cryopreservation placement cavity 3 through the liquid nitrogen valve, so that the cryopreservation tube adapter 4 is quickly frozen. A cryopreservation tube slot 5 is opened on the cryopreservation tube adapter 4, and a cryopreservation tube 6 is arranged in the cryopreservation tube slot 5. The outer periphery of the cryopreservation tube 6 is correspondingly installed with the inner wall of the cryopreservation tube slot 5. The cryopreservation tube 6 can be placed in the cryopreservation tube slot 5. One end of the cryopreservation tube 6 is provided with a tube cap 7, the top end of the cryopreservation tube 6 is provided with a thread, and the tube cap 7 is correspondingly installed with the top end of the cryopreservation tube 6. The tube cap 7 is threadedly connected to the cryopreservation tube 6. The settings of the tube cap 7 and the cryopreservation tube 6 can store immune cells at low temperature, thereby ensuring the quality and integrity of immune cells. The settings of the cryopreservation box 1, the cryopreservation tube adapter 4, the cryopreservation tube 6 and the tube cap 7 can cooperate with each other, so as to more accurately and quickly ensure the temperature of stored immune cells, and further ensure that immune cells can maintain vitality throughout the freezing process;

[0024] A positioning seat 8 is arranged inside the cryopreservation box 1. The installation end of the positioning seat 8 is correspondingly installed at the middle position of the inner cavity of the cryopreservation box 1. The installation end of the positioning seat 8 is fixedly connected to the cryopreservation box 1. A positioning port 12 is opened above the positioning seat 8. A positioning tube 9 is arranged below the sealing cover 2. The installation end of the positioning tube 9 is correspondingly installed at the middle position of one side of the sealing cover 2. The installation end of the positioning tube 9 is fixedly connected to one side of the sealing cover 2. A positioning column 15 is arranged inside the positioning tube 9. One end of the positioning column 15 is fixedly connected to the positioning tube 9. A gap is left between the inner circle of the positioning tube 9 and the outer periphery of the positioning column 15. The outer periphery of the positioning column 15 is mutually attached to the inner wall of the positioning port 12. The outer periphery of the positioning seat 8 is mutually attached to the inner wall of the positioning tube 9. The arrangements of the positioning seat 8 and the positioning tube 9 can make the sealing cover 2 more stable when covering the cryopreservation box 1, and at the same time avoid the situation that the sealing cover 2 rotates randomly when covering the cryopreservation box 1, resulting in unstable temperature inside the cryopreservation box 1. A cover ear 10 is arranged on the other side of the sealing cover 2. One side of the cover ear 10 is correspondingly installed with one side of the sealing cover 2. One side of the cover ear 10 is fixedly connected to the sealing cover 2. The outer periphery of the cover ear 10 is arranged in a wavy shape. The arrangement of the cover ear 10 is convenient for the operator to pick up and place the sealing cover 2, which is beneficial for the operator to take the immune cells. A connecting column 13 is arranged below the cryopreservation box 1. The installation end of the connecting column 13 is correspondingly installed at one side below the cryopreservation box 1. The installation end of the connecting column 13 is fixedly connected to the lower part of the cryopreservation box 1. The top end of the connecting column 13 is arranged in a polygonal shape. A connecting hole 14 is opened above the cover ear 10. The connecting column 13 and the connecting hole 14 are mutually adapted. The arrangements of the connecting column 13 and the connecting hole 14 can enable multiple sets of this device to be stacked and placed, thereby reducing the space occupied when this device stores and places immune cells. The overall structure of this device is simple and the operation is convenient. It can stably place immune cells, and at the same time can ensure the low-temperature state of immune cells in the cryopreservation state, thereby ensuring the viability state of immune cells. At the same time, multiple sets of this device can be stacked to reduce space occupancy, and it is greatly convenient for the operator to pick up and place immune cells.

[0025] In this embodiment, first, the immune cells to be cryopreserved are placed in a culture medium with nutrients and growth factors, then the immune cells are transferred into the cryopreservation tube 6, and then the cryopreservation tube 6 is capped with the tube cap 7. Then, the cryopreservation tube 6 containing the immune cells is placed corresponding to the cryopreservation tube slot 5 on the cryopreservation tube adapter 4. Then, the cryopreservation box 1 is hermetically covered with the sealing cover 2. Then, liquid nitrogen is injected into the cryopreservation chamber 3 through the liquid nitrogen valve in the cryopreservation chamber 3, so that the immune cells are quickly cooled to near the freezing point and ensure that they can maintain their viability throughout the freezing process. The cells are quickly frozen in the low-temperature medium, ensuring the stability of the low temperature inside the cryopreservation box 1, thereby completing the cryopreservation operation of the immune cells.

[0026] The above specific embodiments are merely a preferred embodiment of the present utility model. Based on the technical solution of the present utility model and the relevant revelations of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. An immunocyte cryopreservation device, comprising a cryopreservation box (1), characterized in that: A sealing cover (2) is arranged above the cryopreservation box (1). A plurality of cryopreservation placement cavities (3) are formed in the cryopreservation box (1). A cryopreservation tube adapter (4) is arranged in each cryopreservation placement cavity (3). A cryopreservation tube slot (5) is formed in the cryopreservation tube adapter (4). A cryopreservation tube (6) is arranged in the cryopreservation tube slot (5). A tube cap (7) is arranged at one end of the cryopreservation tube (6). A limiting seat (8) is arranged in the cryopreservation box (1). A limiting tube (9) is arranged below the sealing cover (2). A cover ear (10) is arranged on the other side of the sealing cover (2). A connecting column (13) is arranged below the cryopreservation box (1).

2. The cryopreservation device for immune cells according to claim 1, wherein The inner cavity of the cryopreservation box (1) is concave. A sealing ring groove (11) is formed in the cryopreservation box (1). The edge position of the sealing cover (2) is correspondingly installed with the sealing ring groove (11).

3. The cryopreservation device for immune cells according to claim 1, characterized in that, The installation end of the cryopreservation tube adapter (4) is correspondingly installed with the inner cavity of the cryopreservation placement cavity (3). The outer periphery of the cryopreservation tube adapter (4) is in mutual fit with the inner wall of the cryopreservation placement cavity (3).

4. An immunocyte cryopreservation device according to claim 1, wherein The outer periphery of the cryopreservation tube (6) is correspondingly installed with the inner wall of the cryopreservation tube slot (5). The top end of the cryopreservation tube (6) is provided with a thread. The tube cap (7) is correspondingly installed with the top end of the cryopreservation tube (6).

5. The cryopreservation device for immune cells according to claim 1, wherein The installation end of the limiting seat (8) is correspondingly installed with the middle position of the inner cavity of the cryopreservation box (1). A limiting port (12) is formed above the limiting seat (8).

6. The cryopreservation device for immune cells according to claim 5, characterized in that, The installation end of the limiting tube (9) is correspondingly installed with the middle position of one side of the sealing cover (2). A positioning column (15) is arranged in the limiting tube (9). The outer periphery of the positioning column (15) is in mutual fit with the inner wall of the limiting port (12). The outer periphery of the limiting seat (8) is in mutual fit with the inner wall of the limiting tube (9).

7. An immunocyte cryopreservation device according to claim 1, wherein, One side of the cover ear (10) is correspondingly installed with one side of the sealing cover (2). The outer periphery of the cover ear (10) is arranged in a wavy shape.

8. An immunocyte cryopreservation device according to claim 1, wherein, The installation end of the connecting column (13) is correspondingly installed with one side below the cryopreservation box (1). The top end of the connecting column (13) is arranged in a polygonal shape. A connecting hole (14) is formed above the cover ear (10). The connecting column (13) and the connecting hole (14) are mutually adapted.

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