Exosome extraction device

Abstract

The utility model relates to the technical field of extraction devices, in particular to an exosome extraction device. The problem that in the prior art, a test tube and a containing groove are not tightly matched after long-time work is solved, and the practicability of equipment is improved. The device structurally comprises a mounting box body, a first mounting groove is formed in the top of the mounting box body, a rotating part is rotationally connected into the first mounting groove, a driving structure used for driving the rotating part to rotate is arranged in the box body, a plurality of test tubes are arranged on the rotating part, and each test tube abuts against a first clamping part and a second clamping part; a first connecting structure for controlling the first clamping piece to be close to or away from the test tube is arranged between each first clamping piece and the rotating piece. According to the embodiment of the invention, even if the first clamping piece and the second clamping piece are not tightly matched with the test tube in the mounting box body, the first clamping piece and the second clamping piece can be controlled to tightly clamp the test tube through the synchronous structure. The embodiment of the utility model has the advantages of long service life and strong practicability.

Description

Technical Field

[0001] The utility model relates to the technical field of extraction devices, and more specifically, to an exosome extraction device. Background Technique

[0002] An exosome extraction device is a device used to extract exosomes from cell culture media, plasma, or other biological fluids. It separates exosomes from the mixture through an ultracentrifuge for enrichment and purification, which is of great significance for medical and biological research.

[0003] The operation of the existing exosome extraction device is as follows: First, place the test tube filled with the mixture into the placement groove inside the extraction device, then perform centrifugal rotation on the test tube. After a period of time, take out the test tube and extract the part containing exosomes in the test tube, and then repeat the above operations on the extracted exosomes for centrifugal purification.

[0004] Although this can achieve the separation of exosomes from the mixture, there are still the following problems: Since the test tube needs to be taken and placed in the placement groove multiple times each time exosomes are extracted, after a long time of operation, the fit between the test tube and the placement groove may become loose, resulting in the test tube being difficult to adapt to high-speed centrifugal rotation. Content of the Utility Model

[0005] The utility model provides an exosome extraction device, which solves the problem that the fit between the test tube and the placement groove is likely to become loose after long-term operation in the prior art, and improves the practicability of the device.

[0006] The technical solution of the utility model is as follows:

[0007] An exosome extraction device includes an installation box body.

[0008] A first installation groove is provided at the top of the installation box body. A rotating member is rotatably connected in the first installation groove. A driving structure for driving the rotating member to rotate is provided inside the box body. A plurality of test tubes are provided on the rotating member. A first clamping member and a second clamping member are abutted against each test tube. A first connection structure for controlling the first clamping member to approach or move away from the test tube is provided between each first clamping member and the rotating member. A second connection structure for controlling the second clamping member to approach or move away from the test tube is provided between each second clamping member and the rotating member. A synchronization structure for synchronously controlling each first connection structure and each second connection structure is further provided on the rotating member. A sealing cover is rotatably connected to the top of the installation box body.

[0009] Furthermore, the first connection structure includes a first connection block, which is fixedly connected to the rotating member. A sliding rod is slidably connected to the first connection block and is also slidably connected to the rotating member. The other end of the sliding rod is fixed with a second connection block. A first connecting rod is provided between the first connection block and the first clamping member, and both ends of the first connecting rod are rotatably connected to the first connection block and the first clamping member respectively. A second connecting rod is provided between the second connection block and the first clamping member, and both ends of the second connecting rod are rotatably connected to the second connection block and the first clamping member respectively.

[0010] Furthermore, a limiting spring is also sleeved on the sliding rod, and both ends of the limiting spring are fixedly connected to the first connection block and the second connection block respectively. The first clamping structure and the second clamping structure are identical in structure.

[0011] Furthermore, the synchronization structure includes a limiting cover. A second installation groove is also provided at the top of the rotating member, and the limiting cover is threadedly connected to the second installation groove. The lower end of the limiting cover abuts against the second connection block.

[0012] Furthermore, a rotating member is provided at the top of the limiting cover, and a plurality of rotating grooves are also provided on the limiting cover, and the rotating grooves correspond to the test tubes one by one.

[0013] Furthermore, clamping grooves are provided on both the first clamping member and the second clamping member. The clamping grooves are of an arc-shaped structure, and a buffer layer is also provided on the clamping grooves. The buffer layer is made of sponge material.

[0014] Furthermore, the driving structure includes a driving motor, which is fixedly connected to the inner wall of the installation box body. The output shaft of the driving motor is fixedly connected to the rotating member.

[0015] Furthermore, third installation grooves and fourth installation grooves are also provided on both sides of the top of the installation box body. A first telescopic member is provided in the third installation groove. The fixed end of the first telescopic member is rotatably connected to the inner wall of the third installation groove, and the telescopic end of the first telescopic member is rotatably connected to the sealing cover. A second telescopic member is provided in the fourth installation groove. The fixed end of the second telescopic member is rotatably connected to the inner wall of the fourth installation groove, and the telescopic end of the second telescopic member is rotatably connected to the sealing cover.

[0016] The working principle and beneficial effects of the present utility model are as follows:

[0017] Working process of this embodiment: First, open the sealing cover by extending the first telescopic member and the second telescopic member. Then, place the test tube filled with the mixed solution into the space between the first clamping member and the second clamping member through the rotating groove. Next, rotate the rotating member to lower the sealing cover, and make the first connecting block approach the second connecting block, so that the first clamping member and the second clamping member clamp and fix the test tube. Then, contract the first telescopic member and the second telescopic member to seal the installation box body with the sealing cover. Finally, start the driving motor to perform centrifugal rotation on the test tube. After the centrifugal rotation is completed, reopen the sealing cover, rotate the rotating member, and take out the test tube to complete the separation of exosomes in the single mixed solution.

[0018] In this embodiment, a first clamping member and a second clamping member are arranged in the installation box body, and the synchronous clamping and fixing of multiple test tubes are completed through the first connection structure, the second connection structure and the synchronous structure. Even if the cooperation between the first clamping member and the second clamping member in the installation box body is not tight for the test tube, this embodiment can also control the first clamping member and the second clamping member to tightly clamp the test tube through the synchronous structure. This embodiment has a long service life and strong practicability. Description of the Drawings

[0019] The following further elaborates on the present utility model in detail in conjunction with the drawings and specific embodiments.

[0020] Figure 1 Schematic structural diagram of the prior art;

[0021] Figure 2 Schematic diagram of the overall structure of this embodiment;

[0022] Figure 3 Schematic diagram of the internal structure of this embodiment;

[0023] Figure 4 Schematic diagram of the connection structure of the first clamping member and the second clamping member in this embodiment;

[0024] Figure 5 Schematic diagram of the structures of the first clamping member and the second clamping member in this embodiment.

[0025] In the figure:

[0026] 1. Installation box body; 11. First installation groove; 12. Third installation groove; 13. Fourth installation groove; 2. Sealing cover; 3. Rotating member; 31. Second installation groove; 4. Limiting cover; 41. Rotating member; 42. Rotating groove; 5. Test tube; 61. First telescopic member; 62. Second telescopic member; 71. First clamping member; 711. First connecting rod; 712. Second connecting rod; 713. Clamping groove; 7131. Buffer layer; 72. Second clamping member; 8. Driving motor; 91. First connecting block; 92. Second connecting block; 93. Sliding rod; 931. Limiting spring. Detailed implementation manners

[0027] Next, in combination with the embodiments of the present utility model, the technical solutions in the embodiments of the present utility model will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present utility model.

[0028] As Figures 2 to 5 shown, this embodiment proposes an exosome extraction device, the structure of which includes an installation box body 1. The first installation groove 11 in this embodiment is arranged at the top of the installation box body 1, the rotating member 3 is rotatably arranged in the first installation groove 11, and a driving structure is arranged in the box body to drive the rotating member 3 to rotate. A plurality of test tubes 5 are arranged on the rotating member 3, the first clamping member 71 and the second clamping member 72 are abutted against each test tube 5, a first connection structure is arranged between each first clamping member 71 and the rotating member 3 to control the first clamping member 71 to approach or move away from the test tube 5. A second connection structure is arranged between each second clamping member 72 and the rotating member 3 to control the second clamping member 72 to approach or move away from the test tube 5. A synchronization structure is arranged on the rotating member 3 to synchronously control each first connection structure and each second connection structure. The sealing cover 2 is rotatably arranged at the top of the installation box body 1.

[0029] The first connection structure in this embodiment includes a first connection block 91, and the first connection block 91 is fixedly connected to the rotating member 3. A sliding rod 93 is slidably connected to the first connection block 91, and the sliding rod 93 is slidably connected to the rotating member 3. A second connection block 92 is fixedly arranged at the other end of the sliding rod 93. The sliding rod 93 is used to restrict the positions of the first connection block 91 and the second connection block 92 to prevent the first connection block 91 and the second connection block 92 from being displaced. A first connecting rod 711 is arranged between the first connection block 91 and the first clamping member 71, and both ends of the first connecting rod 711 are rotatably connected to the first connection block 91 and the first clamping member 71 respectively. A second connecting rod 712 is arranged between the second connection block 92 and the first clamping member 71, and both ends of the second connecting rod 712 are rotatably connected to the second connection block 92 and the first clamping member 71 respectively. Such a design ensures that when the first connection block 91 and the second connection block 92 move closer, the first clamping member 71 can move in a direction away from the sliding rod 93 to complete the clamping work of the test tube 5, so that when the user thinks that the clamping of the test tube 5 is loose, the effect of quickly clamping the test tube 5 can be completed by adjusting the distance between the first connection block 91 and the second connection block 92.

[0030] In this embodiment, the limiting spring 931 is sleeved on the sliding rod 93. The two ends of the limiting spring 931 are fixedly connected to the first connecting block 91 and the second connecting block 92 respectively. The first clamping structure and the second clamping structure have the same structure. Such a design ensures that the first connecting block 91 and the second connecting block 92 will move away from each other without being restricted by other external forces. Only by controlling the movement of the second connecting block 92 or the first connecting block 91 towards each other can the function of clamping the test tube 5 be realized.

[0031] The synchronization structure in this embodiment includes a limiting cover 4. The second installation groove 31 is arranged at the top of the rotating part 3. The limiting cover 4 is threadedly connected to the second installation groove 31. The lower end of the limiting cover 4 abuts against the second connecting block 92. In this embodiment, since the limiting cover 4 is threadedly connected to the second installation groove 31, after the distance between the first clamping part 71 and the second clamping part 72 reaches the appropriate position, the position of the limiting cover 4 can be locked and fixed by threading, ensuring that the distance between the first clamping part 71 and the second clamping part 72 remains unchanged after the adjustment is completed.

[0032] Clamping grooves 713 are provided on both the first clamping part 71 and the second clamping part 72 in this embodiment. The clamping groove 713 is an arc-shaped structure, which is used to increase the contact area between the clamping groove 713 and the test tube 5, making the clamping between the clamping groove 713 and the test tube 5 more firm and reliable. A buffer layer 7131 is arranged on the clamping groove 713. The buffer layer 7131 is made of sponge material. In this embodiment, due to the softness and large feasible deformation of the sponge itself, the design of the buffer layer 7131 can effectively reduce the collision damage of the first clamping part 71 and the second clamping part 72 to the test tube 5.

[0033] The rotating part 41 in this embodiment is arranged on the top of the limiting cover 4, which is used to facilitate the rotation of the limiting rod. A number of rotating grooves 42 are arranged on the limiting cover 4. The rotating grooves 42 correspond to the test tubes 5 one by one. The design of the rotating grooves 42 in this embodiment is to reduce the collision damage of the limiting cover 4 to the test tube 5 during the process of clamping and limiting the test tube 5 by rotating the limiting cover 4 after the test tube 5 is placed in the rotating groove 42.

[0034] In this embodiment, the third installation groove 12 and the fourth installation groove 13 are arranged on both sides of the top of the installation box body 1 for placing the first telescopic member 61 and the second telescopic member 62, ensuring that the sealing cover 2 is more tightly sealed when sealing the installation box body 1. The first telescopic member 61 is arranged in the third installation groove 12. The fixed end of the first telescopic member 61 is rotatably connected to the inner wall of the third installation groove 12, and the telescopic end of the first telescopic member 61 is rotatably connected to the sealing cover 2. The second telescopic member 62 is arranged in the fourth installation groove 13. The fixed end of the second telescopic member 62 is rotatably connected to the inner wall of the fourth installation groove 13, and the telescopic end of the second telescopic member 62 is rotatably connected to the sealing cover 2. The first telescopic member 61 and the second telescopic member 62 adopted in this embodiment are preferably hydraulic cylinders or air cylinders. Among them, both hydraulic cylinders and air cylinders are prior arts and will not be elaborated in this embodiment. In this embodiment, the first telescopic member 61 and the second telescopic member 62 are used together to support the rotation of the sealing cover 2, so that both ends of the sealing cover 2 rotate synchronously, which is more stable and reliable.

[0035] The drive structure in this embodiment includes a drive motor 8. The drive motor 8 is fixedly connected to the inner wall of the installation box body 1, and the output shaft of the drive motor 8 is fixedly connected to the rotating member 3. In this embodiment, the drive motor 8 is placed inside the installation box body 1, which reduces the influence of external factors such as dust on the drive motor 8 and increases the service life of the drive motor 8.

[0036] The working process of this embodiment: First, open the sealing cover 2 by extending the first telescopic member 61 and the second telescopic member 62. Then, place the test tube 5 filled with the mixed liquid into the space between the first clamping member 71 and the second clamping member 72 through the rotating groove 42. Then, make the sealing cover 2 move downward by rotating the rotating member 41, and move the first connecting block 91 closer to the second connecting block 92, so that the first clamping member 71 and the second clamping member 72 clamp and fix the test tube 5. Then, contract the first telescopic member 61 and the second telescopic member 62 to seal the installation box body 1 with the sealing cover 2, and then start the drive motor 8 to centrifuge and rotate the test tube 5. After the centrifugal rotation is completed, open the sealing cover 2 again, rotate the rotating member 41 and take out the test tube 5 to complete the separation of exosomes in the single mixed liquid.

[0037] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An exosome extraction device, including an installation box body (1), characterized in that a first installation groove (11) is provided at the top of the installation box body (1), a rotating member (3) is rotatably connected in the first installation groove (11), a driving structure for driving the rotating member (3) to rotate is provided in the box body, a plurality of test tubes (5) are provided on the rotating member (3), a first clamping member (71) and a second clamping member (72) are abutted on each test tube (5), a first connection structure for controlling the first clamping member (71) to approach or move away from the test tube (5) is provided between each first clamping member (71) and the rotating member (3), a second connection structure for controlling the second clamping member (72) to approach or move away from the test tube (5) is provided between each second clamping member (72) and the rotating member (3), a synchronization structure for synchronously controlling each first connection structure and each second connection structure is further provided on the rotating member (3), and a sealing cover (2) is rotatably connected to the top of the installation box body (1).

2. The exosome extraction device according to claim 1, wherein, The first connection structure includes a first connection block (91), the first connection block (91) is fixedly connected to the rotating member (3), a sliding rod (93) is slidably connected to the first connection block (91), the sliding rod (93) is slidably connected to the rotating member (3), the other end of the sliding rod (93) is fixed with a second connection block (92), a first connecting rod (711) is provided between the first connection block (91) and the first clamping member (71), and both ends of the first connecting rod (711) are rotatably connected to the first connection block (91) and the first clamping member (71) respectively. A second connecting rod (712) is provided between the second connection block (92) and the first clamping member (71), and both ends of the second connecting rod (712) are rotatably connected to the second connection block (92) and the first clamping member (71) respectively.

3. The exosome extraction device according to claim 2, wherein, A limiting spring (931) is further sleeved on the sliding rod (93), both ends of the limiting spring (931) are fixedly connected to the first connection block (91) and the second connection block (92) respectively, and the first clamping structure and the second clamping structure are the same.

4. The exosome extraction device according to claim 3, characterized in that, The synchronization structure includes a limiting cover (4), a second installation groove (31) is further provided at the top of the rotating member (3), the limiting cover (4) is threadedly connected to the second installation groove (31), and the lower end of the limiting cover (4) abuts against the second connection block (92).

5. An exosome extraction device according to claim 1, characterized in that, A rotating member (41) is further provided at the top of the limiting cover (4), and a plurality of rotating grooves (42) are provided on the limiting cover (4), and the rotating grooves (42) correspond to the test tubes (5) one by one.

6. The exosome extraction device according to claim 1, wherein, Clamping grooves (713) are provided on both the first clamping member (71) and the second clamping member (72), the clamping grooves (713) are arc-shaped structures, and a buffer layer (7131) is further provided on the clamping grooves (713), and the buffer layer (7131) is made of sponge material.

7. The exosome extraction device according to claim 1, characterized in that, The driving structure includes a driving motor (8), the driving motor (8) is fixedly connected to the inner wall of the installation box body (1), and the output shaft of the driving motor (8) is fixedly connected to the rotating member (3).

8. An exosome extraction device according to claim 1, characterized in that, On both sides of the top of the installation box body (1), a third installation groove (12) and a fourth installation groove (13) are further provided. A first telescopic member (61) is arranged in the third installation groove (12). The fixed end of the first telescopic member (61) is rotatably connected to the inner wall of the third installation groove (12), and the telescopic end of the first telescopic member (61) is rotatably connected to the sealing cover (2). A second telescopic member (62) is arranged in the fourth installation groove (13). The fixed end of the second telescopic member (62) is rotatably connected to the inner wall of the fourth installation groove (13), and the telescopic end of the second telescopic member (62) is rotatably connected to the sealing cover (2).

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