Cell stress loading system

Abstract

The utility model provides a cell stress loading system, which relates to the technical field of medical experimental instruments and comprises a controller, and a tensile stress loading device, a shear stress loading device and a normal stress loading device which are respectively connected with the controller, the cell culture dish is placed in the flowing cavity; a liquid inlet and a liquid outlet are formed in two opposite sides of the flowing cavity; the inlet and the outlet are respectively communicated with a liquid inlet pipeline and a liquid outlet pipeline; the liquid inlet pipeline is communicated with the liquid storage tank; the tensile stress loading equipment is used for stretching and shrinking the cell culture dish; the shear stress loading equipment is arranged at the inlet end and is used for adjusting the flow rate of liquid entering the flowing cavity; the normal stress loading devices are arranged at the inlet end and the outlet end and used for adjusting the liquid flow in the flowing cavity. The equipment can induce various cells and tissues to change under the action of tensile stress, normal stress and shear stress, more complex mechanical variable control is carried out, and the research on cell characteristics under complex mechanical conditions is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of medical experimental instruments, in particular to a cell stress loading system. Background Technique

[0002] With the development of tissue engineering and biomedical engineering, cell mechanics, tissue engineering, etc. have become the forefront disciplines in the development of modern biology. The growth and development of body tissues and cells are in a complex mechanical microenvironment. Mechanical stimulation plays an important regulatory role in the physiological, pathological states and function execution of cells. Therefore, the changes in the structure and function of cells under mechanical stress and the related signal transduction under stress stimulation are important research contents of cell mechanics. In order to study the effects of various physical and biochemical factors on human tissues, a suitable cell stress loading device is a necessary condition for carrying out relevant research.

[0003] Since different cells require different stress conditions in the body, at present, in order to simulate the real stress conditions of cells, existing cell stress loading devices are respectively used to stimulate the tensile stress, compressive stress, shear stress, etc. of isolated cells. At the same time, the existing technology can already perform quantitative mechanical loading on multiple groups of cells. However, existing devices can only apply one or two mechanical stresses to the cells in the culture dish, such as tensile stress and / or compressive stress, and cannot perform more complex mechanical variable control, which is not conducive to the study of cell characteristics under complex mechanical conditions. Content of the Utility Model

[0004] The purpose of the utility model is to provide a cell stress loading system to alleviate the technical problems in the existing technology that only one or two mechanical stresses can be applied to the cells in the culture dish, more complex mechanical variable control cannot be performed, and it is not conducive to the study of cell characteristics under complex mechanical conditions.

[0005] The cell stress loading system provided by the utility model includes a controller, a tensile stress loading device, a shear stress loading device and a normal stress loading device respectively connected to the controller; a flow cavity; a cell culture dish is placed in the flow cavity; liquid inlets and outlets are arranged on two opposite sides of the flow cavity; the inlets and outlets are respectively communicated with a liquid inlet pipe and a liquid outlet pipe; the end of the liquid inlet pipe far from the inlet is communicated with a liquid storage tank; the tensile stress loading device is used to stretch and contract the cell culture dish; the shear stress loading device is arranged at the inlet end and is used to adjust the liquid flow rate entering the flow cavity; the normal stress loading device is arranged at the inlet end and the outlet end and is used to adjust the liquid flow rate in the flow cavity.

[0006] Further, the normal stress loading device includes two sets of corresponding lifting mechanisms and baffles, and guiding grooves cooperating with the baffles are provided at both the inlet and the outlet; the lifting mechanisms are respectively connected to the flow cavity and the baffles, and the baffles can move along the guiding grooves to block or expose the inlet and the outlet.

[0007] Further, the lifting mechanism includes a lead screw lifting structure, the driving end of the lead screw lifting structure is fixedly connected to the flow cavity, and the other end is fixedly connected to the baffle; the driving end is used to drive the baffle to move along the extending direction of the guiding groove.

[0008] Further, the normal stress loading device includes two sets of corresponding adjusting plate assemblies and driving mechanisms; the two adjusting plate assemblies respectively cover the inlet and the outlet; the adjusting plate assembly includes a fixed plate and a moving plate, and through holes are correspondingly arranged on the fixed plate and the moving plate; the fixed plate is fixedly connected to the flow cavity, and the driving mechanism is connected to the moving plate to drive the moving plate and the fixed plate to generate relative movement.

[0009] Further, the driving mechanism includes a telescopic driving rod, and the two ends of the telescopic driving rod are respectively fixedly connected to the flow cavity and the moving plate.

[0010] Further, a plurality of through holes are correspondingly arranged on the fixed plate and the moving plate, and the plurality of through holes are evenly spaced.

[0011] Further, the through holes are strip-shaped, and the moving direction of the moving plate is perpendicular to the length extending direction of the through holes.

[0012] Further, the normal stress loading device includes two sets of corresponding stopper assemblies and driving components; the two stopper assemblies respectively cover the inlet and the outlet; the stopper assembly includes two stoppers respectively hinged on both sides of the inlet or both sides of the outlet; the driving component includes two push-pull rods corresponding to the stoppers; the two ends of the push-pull rod are respectively hinged to the flow cavity and the stopper.

[0013] Further, the shear stress loading device includes a delivery pump arranged on the liquid inlet pipe, and the delivery pump is connected to the controller.

[0014] Further, a flow sensor and a flow velocity sensor respectively connected to the controller are arranged in the flow cavity.

[0015] The cell stress loading system provided by the present utility model allows experimenters to simultaneously or selectively use a tensile stress loading device, a shear stress loading device, and a normal stress loading device according to the requirements of experimental conditions after placing the cell culture dish membrane on the cell culture dish during the experiment. The tensile stress loading device stretches and compresses the cell culture dish membrane; the liquid in the liquid storage tank is sequentially passed through the liquid inlet pipe and the inlet into the flow cavity. The shear stress loading device adjusts the magnitude of the shear stress by regulating the flow rate of the liquid entering the flow cavity at the inlet end; the normal stress loading device cooperates at the inlet end and the outlet end to regulate the liquid flow rate in the flow cavity, thereby adjusting the magnitude of the normal stress.

[0016] As can be seen from the above, by using the above devices, it is possible to accurately induce changes in various cells and tissues under the action of tensile stress, normal stress, and shear stress, enabling more complex control of mechanical variables and contributing to the study of cell characteristics under complex mechanical conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the specific embodiments of the present utility model or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0018] Figure 1 It is a partial structural schematic diagram of the cell stress loading system provided by an embodiment of the present utility model;

[0019] Figure 2 It is a top view of the cell stress loading system provided by an embodiment of the present utility model;

[0020] Figure 3 It is a structural schematic diagram of the normal stress loading device provided by an embodiment of the present utility model;

[0021] Figure 4 It is a structural schematic diagram of the normal stress loading device provided by another embodiment of the present utility model.

[0022] Icons: 1-tensile stress loading device; 2-shear stress loading device; 3-normal stress loading device; 4-flow cavity; 5-inlet; 6-outlet; 7-liquid inlet pipe; 8-liquid outlet pipe; 9-liquid storage tank; 10-through hole; 11-controller; 12-flow sensor; 13-flow velocity sensor; 14-driving end; 15-cell culture dish; 101-pulling assembly; 102-fixed part; 103-moving part; 104-screw assembly; 201-delivery pump; 301-lifting mechanism; 302-baffle; 303-screw lifting structure; 304-adjusting plate assembly; 305-driving mechanism; 306-fixed plate; 307-moving plate; 308-telescopic driving rod; 309-block assembly; 310-driving assembly; 311-block; 312-push-pull rod. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments. Obviously, the embodiments described are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] like Figures 1 - 4 As shown, the cell stress loading system provided by the embodiment of the present invention includes a controller 11, a tensile stress loading device 1, a shear stress loading device 2 and a normal stress loading device 3 respectively connected to the controller 11; a flow cavity 4; a cell culture dish 15 is placed in the flow cavity 4; a liquid inlet 5 and an outlet 6 are provided on opposite sides of the flow cavity 4; the inlet 5 and the outlet 6 are respectively connected to the liquid inlet pipe 7 and the liquid outlet pipe 8; the end of the liquid inlet pipe 7 away from the inlet 5 is connected to the liquid storage tank 9; the tensile stress loading device 1 is used to stretch and shrink the cell culture dish 15; the shear stress loading device 2 is arranged at the inlet 5 end, for adjusting the liquid flow rate entering the flow cavity 4; the normal stress loading device 3 is arranged at the inlet 5 end and the outlet 6 end, for adjusting the liquid flow in the flow cavity 4.

[0025] The controller 11 may be provided with a display screen for displaying displacement information generated by the tensile stress loading device 1, flow velocity information generated by the shear stress loading device 2, flow rate information generated by the normal stress loading device 3, the test time, test frequency, and test mode, etc. The connection method and working principle between the controller 11 and each device are conventional and will not be described in detail here.

[0026] like Figure 1As shown in the figure, the tensile stress loading device 1 includes a pulling component 101 and a lead screw component 104. Specifically, the pulling component 101 includes a fixed part 102 and a moving part 103, and the fixed part 102 and the moving part 103 are respectively fixedly connected to the cell culture dish 15. Among them, the fixed part 102 is fixedly connected to the flow cavity 4, the moving part 103 is connected to the lead screw component 104, and can reciprocate along the extension direction of the flow cavity 4 under the drive of the lead screw component 104, so as to stretch and contract the cell culture dish 15.

[0027] As Figure 1 shown in the figure, further, a flow rate sensor 12 and a flow velocity sensor 13 respectively connected to the controller 11 are arranged in the flow cavity 4. The flow rate sensor 12 and the flow velocity sensor 13 respectively transmit the flow rate information and the flow velocity information to the controller 11, so that the controller 11 controls the shear stress loading device 2 and the normal stress loading device 3 according to the actual situation.

[0028] The fluid can be a culture medium. The cell culture dish membrane placed on the cell culture dish 15 for attaching cells can be a silica gel membrane. One end of the liquid outlet pipe 8 far away from the outlet 6 can be communicated with the liquid storage tank 9 to form a liquid circulation.

[0029] In the cell stress loading system provided by the embodiment of the present utility model, during the experiment, after the experimenter places the cell culture dish membrane on the cell culture dish 15, according to the requirements of the experimental conditions, the tensile stress loading device 1, the shear stress loading device 2 and the normal stress loading device 3 can be used simultaneously or selectively. The tensile stress loading device 1 stretches and compresses the cell culture dish membrane; the liquid in the liquid storage tank 9 is sequentially introduced into the flow cavity 4 through the liquid inlet pipe 7 and the inlet 5. The shear stress loading device 2 is at the inlet 5 end, and the magnitude of the shear stress is adjusted by adjusting the flow velocity of the liquid entering the flow cavity 4; the normal stress loading device 3 cooperates at the inlet 5 end and the outlet 6 end to adjust the liquid flow rate in the flow cavity 4, so as to adjust the magnitude of the normal stress.

[0030] As can be seen from the above, by using the above-mentioned devices, it is possible to accurately induce changes in various cells and tissues under tensile stress, normal stress and shear stress, and to perform more complex mechanical variable control, which is helpful for the study of cell characteristics under complex mechanical conditions.

[0031] As Figure 3 shown in the figure, on the basis of the above embodiment, further, the normal stress loading device 3 includes two sets of corresponding lifting mechanisms 301 and baffles 302, and guide grooves cooperating with the baffles 302 are arranged at both the inlet 5 and the outlet 6; the lifting mechanisms 301 are respectively connected to the flow cavity 4 and the baffles 302, and the baffles 302 can move along the guide grooves to block or expose the inlet 5 and the outlet 6.

[0032] Among them, the direction of the guiding groove can be the vertical direction or the horizontal direction. Correspondingly, the moving direction of the baffle 302 is up and down or left and right.

[0033] Specifically, the lifting mechanism 301 can include a gear-rack combination. The rack is fixedly connected to the baffle 302, and the gear is fixedly connected to the flow cavity 4 and meshes with the rack. During use, the gear rotates forward and backward driven by the motor, thereby driving the rack and the baffle 302 to move reciprocally, and further blocking or exposing the inlet 5 and the outlet 6.

[0034] The lifting mechanism 301 can also include a lead screw lifting structure 303. The driving end 14 of the lead screw lifting structure 303 is fixedly connected to the flow cavity 4, and the other end is fixedly connected to the baffle 302; the driving end 14 is used to drive the baffle 302 to move along the extension direction of the guiding groove. During use, the driving end 14 drives the lead screw to move reciprocally, thereby driving the baffle 302 to move reciprocally along the guiding groove, and further blocking or exposing the inlet 5 and the outlet 6. The guiding groove can play a role in limiting and maintaining stable movement.

[0035] It should be noted that the opening sizes of the inlet 5 and the outlet 6 can be set according to the experimental conditions respectively.

[0036] Or, as Figure 4 shown, the normal stress loading device 3 includes two sets of corresponding adjusting plate assemblies 304 and a driving mechanism 305; the two adjusting plate assemblies 304 respectively cover the inlet 5 and the outlet 6; the adjusting plate assembly 304 includes a fixed plate 306 and a moving plate 307, and through holes 10 are correspondingly arranged on the fixed plate 306 and the moving plate 307; the fixed plate 306 is fixedly connected to the flow cavity 4, and the driving mechanism 305 is connected to the moving plate 307 to drive the moving plate 307 and the fixed plate 306 to generate relative movement.

[0037] When the moving plate 307 and the fixed plate 306 completely overlap, the through holes 10 on the two plates correspond and communicate, and at this time the flow rate is the largest. When the moving plate 307 is moved, the corresponding through holes 10 on the two plates are gradually staggered, and the flow rate gradually decreases until the through holes 10 are completely staggered and the inlet 5 and the outlet 6 are closed.

[0038] A plurality of through holes 10 are correspondingly arranged on the fixed plate 306 and the moving plate 307, and the plurality of through holes 10 are evenly spaced.

[0039] Specifically, the through holes 10 can be circular, square, diamond-shaped or any irregular shape.

[0040] Preferably, the through hole 10 is in a strip shape, and the moving direction of the moving plate 307 is perpendicular to the length extension direction of the through hole 10. With this setting, in the case of any opening size, that is, any flow rate, when the fluid enters the flow cavity 4, it can flow in evenly, making the acting force more uniform.

[0041] The driving mechanism 305 includes a telescopic driving rod 308. The two ends of the telescopic driving rod 308 are respectively fixedly connected to the flow cavity 4 and the moving plate 307. The telescopic driving rod 308 can be a hydraulic rod or a pneumatic rod. Alternatively, the driving mechanism 305 can be a combination of a lead screw and a motor. The driving mechanism 305 can drive the moving plate 307 to reciprocate. The telescopic driving rod 308 can push and pull the moving plate 307.

[0042] The driving mechanism 305 can also include a rotating motor. The through holes 10 on the moving plate 307 and the fixed plate 306 are in a fan blade shape, and a plurality of through holes 10 are evenly distributed circumferentially along the center of the plate. The output shaft of the rotating motor is fixedly connected to the center of the moving plate 307, driving the moving plate 307 to rotate, and the overlapping size of the corresponding through holes 10 on the two plates changes. The rotating motor can rotate the moving plate 307.

[0043] Or, as Figure 1 shown, the normal stress loading device 3 includes two sets of corresponding block 311 assemblies 309 and driving assemblies 310; the two block 311 assemblies 309 respectively cover the inlet 5 and the outlet 6; the block 311 assembly 309 includes two blocks 311 respectively hinged on both sides of the inlet 5 or both sides of the outlet 6; the driving assembly 310 includes two push-pull rods 312 corresponding to the blocks 311; the two ends of the push-pull rod 312 are respectively hinged to the flow cavity 4 and the block 311.

[0044] Specifically, the block 311 and the flow cavity 4 are hinged through a hinge. The push-pull rod 312 can be a hydraulic rod or a pneumatic rod, etc. When the push-pull rod 312 gradually extends, it drives the block 311 to move until the block 311 gradually covers the inlet 5 or the outlet 6; when the push-pull rod 312 gradually shortens, it drives the block 311 to move until the block 311 gradually exposes the inlet 5 or the outlet 6. During the experiment, the push-pull rod 312 drives the block 311 to rotate relative to the inlet 5 or the outlet 6, thereby blocking or exposing the inlet 5 or the outlet 6, and further adjusting the flow rate.

[0045] As <s Figure 1 shown, on the basis of the above embodiment, further, the shear stress loading device 2 includes a delivery pump 201 provided on the liquid inlet pipe 7, and the delivery pump 201 is connected to the controller 11.

[0046] In this embodiment, during the experiment, the controller 11 is used to control the liquid pumping intensity of the delivery pump 201, so as to control the flow rate of the liquid entering the inlet 5.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cell stress loading system, characterized in that, The cell stress loading system includes a controller, a tensile stress loading device, a shear stress loading device, and a normal stress loading device, which are respectively connected to the controller. A flow chamber; a cell culture dish is placed in the flow chamber; an inlet and an outlet for liquid are provided on two opposite sides of the flow chamber; the inlet and the outlet are respectively communicated with a liquid inlet pipe and a liquid outlet pipe; one end of the liquid inlet pipe far from the inlet is communicated with a liquid storage tank. The tensile stress loading device is used to stretch and contract the cell culture dish; the tensile stress loading device includes a pulling component and a lead screw component; the pulling component includes a fixed part and a moving part; the fixed part and the moving part are respectively fixedly connected to the cell culture dish; the fixed part is fixedly connected to the flow chamber, and the moving part is connected to the lead screw component and can make a reciprocating motion along the extension direction of the flow chamber under the drive of the lead screw component, so as to stretch and contract the cell culture dish. The shear stress loading device is arranged at the inlet end and is used to adjust the flow rate of the liquid entering the flow chamber; the shear stress loading device includes a delivery pump arranged on the liquid inlet pipe, and the delivery pump is connected to the controller; the controller is used to control the liquid pumping force of the delivery pump, so as to control the flow rate of the liquid entering the inlet. The normal stress loading device is arranged at the inlet end and the outlet end and is used to adjust the liquid flow rate in the flow chamber.

2. The cell stress loading system according to claim 1, characterized in that, The normal stress loading device includes two sets of corresponding lifting mechanisms and baffles, and guiding grooves matched with the baffles are arranged at both the inlet and the outlet. The lifting mechanisms are respectively connected to the flow chamber and the baffle, and the baffle can move along the guiding groove to block or expose the inlet and the outlet.

3. The cell stress loading system according to claim 2, wherein The lifting mechanism includes a lead screw lifting structure, the driving end of the lead screw lifting structure is fixedly connected to the flow chamber, and the other end is fixedly connected to the baffle; the driving end is used to drive the baffle to move along the extension direction of the guiding groove.

4. The cell stress loading system according to claim 1, wherein The normal stress loading device includes two sets of corresponding adjusting plate assemblies and driving mechanisms; the two adjusting plate assemblies respectively cover the inlet and the outlet. The adjusting plate assembly includes a fixed plate and a moving plate, and through holes are correspondingly arranged on the fixed plate and the moving plate; the fixed plate is fixedly connected to the flow chamber, and the driving mechanism is connected to the moving plate to drive the moving plate and the fixed plate to generate relative movement.

5. The cell stress loading system according to claim 4, wherein The driving mechanism includes a telescopic driving rod, and two ends of the telescopic driving rod are respectively fixedly connected to the flow chamber and the moving plate.

6. The cell stress loading system according to claim 4, wherein A plurality of the through holes are correspondingly arranged on the fixed plate and the moving plate, and the plurality of through holes are evenly spaced.

7. The cell stress loading system according to claim 6, wherein The through holes are strip-shaped, and the moving direction of the moving plate is perpendicular to the length extension direction of the through holes.

8. The cell stress loading system according to claim 1, characterized in that The normal stress loading device includes two sets of corresponding stop block assemblies and driving components; the two stop block assemblies respectively cover the inlet and the outlet. The stopper assembly includes two stoppers respectively hinged on both sides of the inlet or both sides of the outlet; the driving assembly includes two push-pull rods correspondingly arranged with the stoppers; both ends of the push-pull rod are respectively hinged to the flow cavity and the stopper.

9. The cell stress loading system according to any one of claims 1-8, characterized in that, A flow rate sensor and a flow velocity sensor respectively connected to the controller are arranged in the flow cavity.

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