Rushi inoculation needle

Abstract

The rust fungus inoculation needle comprises a needle body and a needle head, the needle body is in a long rod shape, the needle head is located at one end of the needle body, the needle head is connected to the needle body, the needle head is provided with a smear scraping piece, the edge line of the smear scraping piece extends smoothly, the two sides, in the thickness direction, of the smear scraping piece are surfaces extending smoothly, and the surfaces of the smear scraping piece extend smoothly. At least one side of the scraping sheet along the width direction is a blade part; wherein the width direction of the scraping sheet is perpendicular to the length direction of the needle body. The blade parts are arranged on the two sides of the rust fungus inoculation needle, so that uredospores can be conveniently scraped from a standard sample, a proper amount of uredospores or spore suspension liquid is attached to the scraping smear, then the scraping smear is slightly contacted with the surface of a plant leaf and scrapes a part needing to be inoculated, and the spores can be uniformly attached to the front surface of the leaf, so that the spores are uniformly inoculated. The rust fungus inoculation needle is simple in structure, easy, convenient and efficient to operate and capable of effectively assisting researchers in studying rust diseases.

Description

Technical Field

[0001] The utility model relates to a tool used in the agricultural field, in particular to a rust fungus inoculation needle. Background Art

[0002] Rust is a fungal disease that significantly impacts plant production. For example, wheat stripe rust is a significant threat to wheat growth. Timely and accurate inoculation tests are crucial for studying and preventing this disease. However, there is a lack of inoculation tools specifically designed for rust fungi. Using common rods for rust inoculation tests can be challenging, resulting in inconvenient operation, low efficiency, and potential plant damage. Utility Model Content

[0003] In order to solve one of the above technical problems, the present application provides a rust fungus inoculation needle, which improves the accuracy and efficiency of inoculation and reduces damage to plants.

[0004] The utility model adopts the following technical solutions:

[0005] A rust fungus inoculation needle, comprising:

[0006] A needle body, the needle body being in the shape of a long rod;

[0007] A needle head, the needle head is located at one end of the needle body and is connected to the needle body. The needle head has a scraping blade, the edge line of the scraping blade extends smoothly, both sides of the scraping blade in the thickness direction are smoothly extended surfaces, and at least one side of the scraping blade in the width direction is a blade; wherein the width direction of the scraping blade is perpendicular to the length direction of the needle body.

[0008] Optionally, the thickness of the scraper blade gradually decreases in the direction from the middle portion to the edge portions on both sides of the scraper blade.

[0009] Optionally, both side surfaces of the scraper blade along the thickness direction are flat surfaces or convex curved surfaces.

[0010] Optionally, the thickness of the edge of the scraper blade at one end facing away from the needle body is not less than the thickness of the edge of the blade.

[0011] Optionally, two side surfaces of the scraper blade along the thickness direction are respectively a first side surface and a second side surface;

[0012] The first side surface includes a main surface and an edge surface, wherein the edge surface is located on one side of the main surface along the width direction of the scraper blade;

[0013] There is an angle between the edge surface and the second side surface;

[0014] Wherein, the angle is 10° to 30°.

[0015] Optionally, the edge surface is a flat surface or a convex arc surface.

[0016] Optionally, the needle body and the needle tip are integrally formed;

[0017] Alternatively, the rust inoculation needle includes a plurality of needle tips, and each of the needle tips is detachably connected to the needle body.

[0018] Optionally, the needle tip has a rod portion, and the scraping smear is connected to one end of the rod portion;

[0019] The other end of the rod portion is detachably connected to the needle body.

[0020] Optionally, friction ribs are provided on the outer wall surface of the rod portion.

[0021] Optionally, the rod portion and the needle body adopt any one of the following connection methods;

[0022] External threads are provided on one of the rod portion and the needle body, and a thread groove is provided on the other, and the rod portion and the needle body are threadedly connected;

[0023] A slot is provided on one of the rod portion and the needle body, and the other is inserted into the slot;

[0024] The rod portion and the needle body are connected and fixed by a connecting member.

[0025] By adopting the above technical solutions, the present application has the following beneficial effects:

[0026] On both sides of the rust inoculation needle of the present application, cutting edges are provided, which can facilitate scraping of urediniospores from the specimen, so that an appropriate amount of urediniospores or spore suspension is attached to the scraping smear. Subsequently, the scraping smear is gently contacted with the surface of the plant leaf and drawn across the inoculation site, and the spores can be evenly attached to the front surface of the leaf. The rust inoculation needle of the present application has a simple structure, is easy and efficient to operate, and can effectively assist scientific researchers in studying rust.

[0027] The following further describes in detail the specific embodiments of the present utility model with reference to the accompanying drawings. Description of the Drawings

[0028] The accompanying drawings, as part of the present application, are used to provide a further understanding of the present utility model. The schematic embodiments and descriptions thereof of the present utility model are used to explain the present utility model, but do not constitute an improper limitation to the present utility model. Obviously, the accompanying drawings in the following description are only some embodiments, and those of ordinary skill in the art can also obtain other accompanying drawings according to these drawings without creative efforts. In the accompanying drawings:

[0029] Figure 1Shows a schematic three-dimensional structure diagram of the first rust inoculation needle provided by an embodiment of the present application;

[0030] Figure 2 Shows a schematic three-dimensional structure diagram of the second rust inoculation needle provided by an embodiment of the present application;

[0031] Figure 3 Shows a cross-sectional view of the first needle tip of the rust inoculation needle provided by an embodiment of the present application;

[0032] Figure 4 Shows the state of Figure 3 scraping urediniospores on the specimen using the first needle tip shown;

[0033] Figure 5 Shows the state of Figure 3 transferring urediniospores to a plant leaf using the first needle tip shown;

[0034] Figure 6 Shows a cross-sectional view of the second needle tip of the rust inoculation needle provided by an embodiment of the present application;

[0035] Figure 7 Shows the state of Figure 6 scraping urediniospores on the specimen using the second needle tip shown;

[0036] Figure 8 Shows the state of Figure 6 transferring urediniospores to a plant leaf using the first needle tip shown.

[0037] In the figure: 100, rust inoculation needle; 1, needle body; 11, thread groove; 2, needle tip; 21, scraping smear; 211, cutting edge; 212, first side surface; 2121, main surface; 2122, edge surface; 213, second side surface; 22, rod part; 221, external thread; a, friction rib; b, urediniospores; c, specimen; d, plant leaf.

[0038] It should be noted that these drawings and textual descriptions do not aim to limit the scope of the concept of the present utility model in any way, but rather to illustrate the concept of the present utility model to those skilled in the art by referring to specific embodiments. Detailed implementation manners

[0039] To make the purpose, technical solutions, and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the present utility model. The following embodiments are used to illustrate the present utility model but do not limit the scope of the present utility model.

[0040] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by terms such as "upper", "lower", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or component referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model.

[0041] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installation" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0042] See Figures 1 to 8 As shown, the embodiment of the present application provides a rust inoculation needle 100, which includes: a needle body 1 and a needle tip 2. The needle body 1 is in the shape of a long rod. The needle body 1 can be made of a metal material with corrosion resistance and high hardness, having a good hand feeling and ensuring its stability and durability during use. The needle tip 2 is located at one end of the needle body 1, and the needle tip 2 is connected to the needle body 1. The needle tip 2 has a scraping plate 21. The edge line of the scraping plate 21 extends smoothly. Both sides of the scraping plate 21 in the thickness direction are smooth surfaces extending smoothly. At least one side of the scraping plate 21 in the width direction is a cutting edge 211; wherein, the width direction of the scraping plate 21 is perpendicular to the length direction of the needle body 1.

[0043] During the specific operation process, see Figure 4 As shown, an operator holds the needle body 1 with one hand, rotates the needle body 1 to adjust the angle of the scraping plate 21, so that the scraping plate 21 is close to the surface of the standard sample c. The cutting edge 211 of the scraping plate 21 contacts the surface of the standard sample c. Then, move the rust inoculation needle 100 to transfer the urediniospores b on the standard sample c to the surface of the scraping plate 21. Finally, see Figure 5 As shown, rotate the needle body 1 to adjust the angle of the scraping plate 21, so that the scraping plate 21 is approximately perpendicular to the plant leaf d (the scraping plate 21 and the part of the plant leaf d to be inoculated form an acute angle, close to perpendicular). Gently contact the scraping plate 21 with the surface of the plant leaf d and slide it across the part to be inoculated, so that the spores are evenly attached to the front surface of the leaf.

[0044] The rust fungus inoculation needle 100 of the present application can be provided with cutting edges 211 on both sides in the width direction, which is convenient for scraping urediniospores b from the specimen c, so that an appropriate amount of urediniospores b or spore suspension adheres to the scraping smear 21. Subsequently, the scraping smear 21 is gently contacted with the surface of the plant leaf d and drawn across the inoculation site, so that the spores can be evenly attached to the front of the leaf. The rust fungus inoculation needle 100 of the present application has a simple structure, is easy to operate, efficient, and can effectively assist researchers in studying rust diseases.

[0045] In some possible embodiments, as shown in Figures 3 to 5 shown, in the direction from the middle of the scraping smear 21 to the cutting edges 211 on both sides, the thickness of the scraping smear 21 gradually decreases. That is, the large surface of the scraping smear 21 is thick in the middle and thin on both sides in the width direction. This is conducive to the smooth scraping of urediniospores b on the specimen by the cutting edges 211 on both sides, and the large surface of the scraping smear 21 can be a convex arc surface. The urediniospores b are located on the convex arc surface and are not easy to adhere, which is convenient for transferring to the plant leaf d.

[0046] In some possible embodiments, the two side surfaces of the scraping smear 21 in the thickness direction can also be flat surfaces, but not concave surfaces, to prevent the urediniospores b from falling into the concave structure and being not easy to contact with the plant leaf d.

[0047] In some possible embodiments, the thickness of the edge of the end of the scraping smear 21 away from the needle body 1 is not less than the thickness of the edge of the cutting edge 211. Preferably, the thickness of the edge of the end of the scraping smear 21 away from the needle body 1 is greater than the thickness of the edge of the cutting edge 211. The end of the scraping smear 21 away from the needle body 1 is the end of the rust fungus inoculation needle 100 and is easy to contact the outside world. If the edge is too thin and sharp, there are safety hazards. By designing its thickness to be greater than the thickness of the edge of the cutting edge 211, the end of the scraping smear 21 becomes a blunt edge, reducing the danger. In addition, the end of the scraping smear 21 away from the needle body 1 is not set as a sharp structure, but can be set as a rounded edge, improving the safety.

[0048] In some possible embodiments, as shown in Figures 6 to 8 shown, the two side surfaces of the scraping smear 21 in the thickness direction are respectively the first side surface 212 and the second side surface 213. The first side surface 212 includes a main surface 2121 and an edge surface 2122. The edge surface 2122 is located on one side of the main surface 2121 along the width direction of the scraping smear 21. There is an included angle between the edge surface 2122 and the second side surface 213, and the included angle is 10° - 30°.

[0049] The second side surface 213 and the main surface 2121 are substantially parallel. The edge surface 2122 gradually inclines towards the second side surface 213 and finally connects to the second side surface 213. A cutting edge 211 is formed between the edge surface 2122 and the second side surface 213, and the slope of the edge surface 2122 with respect to the second side surface 213 is small. During the specific operation process, refer to Figure 7 As shown, an operator holds the needle body 1 with one hand, rotates the needle body 1 to adjust the angle of the smear 21, so that the second side surface 213 is close to being parallel to the surface of the standard sample c. Then, move the rust fungus inoculation needle 100. The urediniospores b on the standard sample c can be smoothly transferred to the edge surface 2122. Finally, rotate the needle body 1 to adjust the angle of the edge surface 2122, so that the edge surface 2122 is close to perpendicular to the plant leaf d. Gently touch the smear 21 to the surface of the plant leaf d and draw across the inoculation site, so that the spores are evenly attached to the front of the leaf.

[0050] In some possible embodiments, the edge surface 2122 is a flat straight surface or a convex arc surface. The entire edge surface 2122 is easy to contact with the plant leaf d, and the urediniospores b transferred to the edge surface 2122 can be easily further transferred to the plant leaf d.

[0051] In some possible embodiments, refer to Figure 1 As shown, the needle body 1 and the needle tip 2 are integrally formed. The entire rust fungus inoculation needle 100 has a simple structure, low cost, improves the inoculation accuracy and efficiency, and reduces the damage to the plants.

[0052] In some possible embodiments, refer to Figure 2 As shown, the rust fungus inoculation needle 100 includes multiple needle tips 2, and each of the needle tips 2 is detachably connected to the needle body 1.

[0053] During the experiment, before inoculating the urediniospores b on different standard samples c, the needle tip 2 can be replaced to avoid mutual contamination and affect the experimental results. Compared with preparing multiple rust fungus inoculation needles 100, the method of replacing the needle tip 2 significantly reduces the cost. Each needle body 1 can be used in combination with multiple needle tips 2, and the number of needle tips 2 is more than that of the needle bodies 1.

[0054] In some possible embodiments, refer to Figure 2 As shown, the needle tip 2 has a rod portion 22. The smear 21 is connected to one end of the rod portion 22, and the other end of the rod portion 22 is detachably connected to the needle body 1.

[0055] In addition to having the smear 21, the needle tip 2 also has a rod portion 22. The rod portion 22 can facilitate the operator to hold and avoid the fingers from contacting the smear 21, ensuring safety and hygiene.

[0056] In some possible embodiments, friction ribs a may be provided on the outer wall surface of the rod portion 22. This can increase the frictional force between the hand and the rod portion, facilitating the hand to hold it. Similarly, friction ribs a may also be provided on the needle body 1.

[0057] There can be various different connection methods between the rod portion 22 and the needle body 1;

[0058] In an implementable solution, as shown in Figure 2 One of the rod portion 22 and the needle body 1 is provided with an external thread 221, and the other is provided with a thread groove 11. The rod portion and the needle body 1 are threadedly connected. By providing friction ribs a on the rod portion 22, the frictional force between the finger and the rod portion can be increased, and the operator does not need to apply too much holding force to easily screw the rod portion 22 to achieve the disassembly and assembly of the rod portion 22.

[0059] In another implementable solution, one of the rod portion 22 and the needle body 1 is provided with a slot, and the other is inserted into the slot. The rod portion 22 and the needle body 1 are connected and fixed by a connecting member. Connecting ears may be respectively provided on the rod portion 22 and the needle body 1, and the connecting ears of the two are in contact with each other. The connecting member can connect and fix the two connecting ears. The connecting member can be a screw. The screw rod of the screw penetrates through the two connecting ears, and a nut is threadedly connected to the screw rod. The nut and the cap body on the screw rod are respectively limited on the two connecting ears.

[0060] The following provides the usage method of the rust fungus inoculation needle 100 of the present application:

[0061] 1. Preparation before inoculation:

[0062] Disinfection: Disinfect the rust fungus inoculation needle 100 before use to prevent cross - contamination.

[0063] Prepare the inoculation liquid: Suspend the Puccinia striiformis f. sp. tritici spores in an appropriate amount of water or buffer solution to make a uniform inoculation liquid.

[0064] 2. Inoculation process:

[0065] Gently dip the disinfected rust fungus inoculation needle 100 into the inoculation liquid so that an appropriate amount of spore suspension adheres to the needle tip 2.

[0066] Gently touch the needle tip 2 to the surface of the wheat leaf and draw across the inoculation area, so that the spore suspension uniformly adheres to the leaf. Adjust the inoculation density and inoculation area as needed to ensure the inoculation effect.

[0067] 3. Post - inoculation treatment:

[0068] Place the inoculated wheat plants in a suitable environmental condition for cultivation and observe the inoculation effect. Clean and disinfect the rust fungus inoculation needle 100 for future use.

[0069] The rust inoculation needle 100 of this application is reasonably designed and easy to use. It not only improves the efficiency and accuracy of inoculating Puccinia striiformis f. sp. tritici, but also reduces the damage to plants, and is applicable to disease control work in scientific research and agricultural production.

[0070] The following provides an experimental operation procedure using the rust inoculation needle 100 of this application:

[0071] Seedling raising:

[0072] Select flowerpots with a diameter of 10 cm and a height of 10 cm, and fill them with soil rich in organic matter. Place them in a seedling raising pool, add water, and let the water slowly absorb from the bottom of the flowerpot until the soil surface is completely wet. Sow 20 - 30 healthy and plump susceptible wheat variety "Mingxian 169" in each pot, cover with vermiculite so that there are no exposed seeds, and place them in a greenhouse for cultivation. When the first leaf of the wheat seedling is flattened, it is ready for use.

[0073] Inoculation:

[0074] Use the rust inoculation needle 100 to scrape the urediniospores b on the standard sample c or dip it into fresh urediniospores b, so that an appropriate amount of urediniospores b or spore suspension adheres to the needle tip 2. Gently touch the needle tip 2 to the surface of the wheat leaf, and draw across the inoculation area, so that the spores evenly adhere to the front of the leaf. Insert the label to record the collection information.

[0075] After all the standard samples c are inoculated, spray a layer of water mist on the leaves so that the water droplets do not fall.

[0076] Humidity - maintaining cultivation:

[0077] Transfer the inoculated wheat seedlings into a humidity - maintaining frame (add a layer of water at the bottom of the humidity - maintaining frame). Spray with 0.05% Tween 20 aqueous solution to fully moisturize the inside of the humidity - maintaining frame, cover with a plastic cloth to seal the humidity - maintaining frame. Place it in the dark at 9 - 13 °C for 24 hours for humidity - maintaining. After the humidity - maintaining is completed, take out the inoculated seedlings and place them in the greenhouse for isolated cultivation. The greenhouse cultivation temperature is 14 ± 3 °C, and the light time is 10 - 14 hours per day.

[0078] Clean and disinfect the rust inoculation needle 100 for the next use.

[0079] Treatment of diseased leaves:

[0080] When spores are produced, cut off the non - diseased leaves, trim the heart leaves, and isolate them with a glass cover. After each flowerpot is isolated with a glass cover, continue to place it in the greenhouse for cultivation until spores are produced, then number them separately and collect the strains.

[0081] The above are only the preferred embodiments of the present utility model, and do not impose any formal restrictions on the present utility model. Although the present utility model has been disclosed above with the preferred embodiments, it is not intended to limit the present utility model. Any person skilled in the art of this patent, without departing from the scope of the technical solution of the present utility model, can make some changes or modifications using the technical content prompted above into equivalent embodiments of equivalent changes. However, as long as it does not depart from the content of the technical solution of the present utility model, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present utility model still fall within the scope of the technical solution of the present utility model.

Claims

1. A rust fungus inoculation needle, characterized in that: include: A needle body, the needle body being in the shape of a long rod; A needle head, the needle head is located at one end of the needle body and is connected to the needle body. The needle head has a scraping blade, the edge line of the scraping blade extends smoothly, both sides of the scraping blade in the thickness direction are smoothly extended surfaces, and at least one side of the scraping blade in the width direction is a blade; wherein the width direction of the scraping blade is perpendicular to the length direction of the needle body.

2. The rust fungus inoculation needle according to claim 1, characterized in that The thickness of the doctor blade gradually decreases in a direction from the middle portion of the doctor blade to the edge portions on both sides.

3. The rust fungus inoculation needle according to claim 1, characterized in that The two side surfaces of the scraper blade along the thickness direction are flat surfaces or convex arc surfaces.

4. The rust fungus inoculation needle according to claim 1, characterized in that The thickness of the edge of the scraping blade at one end facing away from the needle body is not less than the thickness of the edge of the blade.

5. The rust fungus inoculation needle according to claim 1, characterized in that The two side surfaces of the scraper blade along the thickness direction are respectively a first side surface and a second side surface; The first side surface includes a main surface and an edge surface, wherein the edge surface is located on one side of the main surface along the width direction of the scraper blade; There is an angle between the edge surface and the second side surface; Wherein, the angle is 10° to 30°.

6. The rust fungus inoculation needle according to claim 5, characterized in that: The edge surface is a flat surface or an outwardly convex arc surface.

7. The rust fungus inoculation needle according to claim 1, characterized in that The needle body and the needle tip are integrally formed; Alternatively, the rust fungus inoculation needle includes a plurality of needle heads, and each of the needle heads is selectively connected to the needle body in a detachable manner.

8. The rust fungus inoculation needle according to claim 7, characterized in that: The needle has a rod portion, and the scraper is connected to one end of the rod portion; The other end of the rod is detachably connected to the needle body.

9. The rust fungus inoculation needle according to claim 8, characterized in that: Friction ribs are arranged on the outer wall surface of the rod portion.

10. The rust fungus inoculation needle according to claim 8, characterized in that: The rod and the needle body are connected in any of the following ways; One of the rod and the needle body is provided with an external thread, and the other is provided with a thread groove, and the rod and the needle body are threadedly connected; One of the rod and the needle body is provided with a slot, and the other is inserted into the slot; The rod portion and the needle body are connected and fixed via a connecting piece.

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