Cell culture fluid filling equipment with leakage-proof structure

Abstract

The utility model relates to the field of cell culture, and discloses cell culture fluid filling equipment with a leakage-proof structure, which comprises a working table, the top end of the working table is fixedly connected with a mounting frame, the top end of the working table is fixedly connected with a fluid storage barrel, the top end of the working table is rotatably connected with a turntable, the inner wall of the turntable is provided with a storage port, and the storage port is provided with a leakage-proof structure. A storage opening is formed in the mounting frame, a culture dish is placed in the storage opening, a filling mechanism is arranged on the inner side of the mounting frame and comprises an air cylinder and a sliding plate, the air cylinder is fixedly connected with the top end of the mounting frame, and the rear end of the sliding plate is slidably connected with the front surface of the mounting frame. According to the device disclosed by the utility model, the filling mechanism is matched with the leakage-proof device for use, so that the material receiving box can move between the turntable and the injection cylinder after the injection cylinder moves to a position higher than the culture dish, and the effect of preventing residual injection on the inner wall of the injection cylinder from dripping onto the turntable after injection is completed is achieved.

Description

Technical Field

[0001] The utility model relates to the field of cell culture, in particular to a cell culture fluid filling device with a leak-proof structure. Background Art

[0002] Cell culture medium is a liquid environment designed specifically for cell growth. It contains various nutrients and regulatory factors required for cell growth, proliferation and maintenance, providing the necessary support and guarantee for in vitro cell culture.

[0003] A search revealed Chinese patent publication number CN212476700U, which discloses a homogenizing and filling device for cell culture fluid. This device, which relates to the field of laboratory experimental fluid filling, includes a rotatable circular worktable and an electric lift. The lift is positioned above the circular worktable, and its upper surface is provided with a plurality of placement slots arranged in a circular array. Culture dishes are placed in the placement slots. A filler is fixedly connected to the front bottom of the lift. The aforementioned application document incorporates a liftable filler positioned above the culture dish. Before the filler is activated, it moves downward toward the dish, allowing for lower filling to prevent splashing of the culture fluid during filling.

[0004] Although the above application documents can effectively prevent the culture fluid from splashing, in actual use, since the bottom of the syringe is opened, after completing a filling, some culture fluid may remain on the inner wall of the syringe below the piston, and the adhesion part is too small, so the inner wall of the bottom tip of the syringe cannot be closed by the adhesion culture fluid, resulting in the adhesion part being unable to remain attached under the action of suction. Therefore, during the process of replacing the culture dish, this part of the residual liquid may drip under the action of gravity, causing the leaked liquid to drip onto the workbench or hit the remaining culture dishes, so that the workbench needs to be cleaned frequently during use, which affects work efficiency. For this reason, a cell culture fluid filling device with a leak-proof structure is proposed to solve the above problem. Utility Model Content

[0005] In order to make up for the above shortcomings, the utility model provides a cell culture fluid filling device with a leak-proof structure, aiming to improve the problem in the prior art that the culture fluid remaining on the inner wall of the syringe may drip after completing a filling process.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a cell culture fluid filling device with a leak-proof structure, comprising a workbench, the top of the workbench is fixedly connected to a mounting frame, the top of the workbench is fixedly connected to a liquid storage barrel, the top of the workbench is rotatably connected to a turntable, the inner wall of the turntable is provided with a storage port, a culture dish is placed inside the storage port, a filling mechanism is provided on the inner side of the mounting frame, the filling mechanism comprises a cylinder and a slide, the cylinder is fixedly connected to the top of the mounting frame, the rear end of the slide is slidably connected to the front surface of the mounting frame, a leak-proof device is provided inside the workbench, the leak-proof device comprises a mounting plate, the rear end of the mounting plate is fixedly connected to the front surface of the mounting frame, and the inner wall of the mounting plate and the inner wall of the mounting frame are jointly provided with an air pressure chamber.

[0007] As a further description of the above technical solution:

[0008] The bottom end of the slide is fixedly connected to a syringe, the inner wall piston of the syringe is connected to a sliding plug, the top of the sliding plug is fixedly connected to the output end of the cylinder, the output end of the cylinder passes through the inner wall of the slide, the output end of the cylinder passes through the inner wall of the syringe, the bottom end of the syringe is fixedly connected to a liquid outlet pipe, a one-way valve 1 is provided inside the liquid outlet pipe, the right side of the syringe is fixedly connected to a liquid inlet pipe, a one-way valve 2 is provided inside the liquid inlet pipe, and the end of the liquid inlet pipe away from the syringe passes through and is fixedly connected to the inner wall of the liquid storage barrel.

[0009] As a further description of the above technical solution:

[0010] The filling mechanism further comprises a baffle, the rear end of which is fixedly connected to the front surface of the mounting frame below the slide plate.

[0011] As a further description of the above technical solution:

[0012] The inner wall piston of the air pressure chamber is connected to a slide bar, a material receiving box is provided at the front end of the slide bar, the slide bar and the material receiving box are fixed by a connecting assembly, the connecting assembly includes a fixing bar, the outer wall of the fixing bar passes through and is fixedly connected to the inner wall of the material receiving box, the inner wall of the fixing bar is provided with a mounting groove, the inner wall piston of the air pressure chamber is connected to a control block, and the top of the control block is elastically connected to the inner wall of the air pressure chamber by a spring.

[0013] As a further description of the above technical solution:

[0014] The inner wall of the mounting groove is slidably connected with a card block, the front end of the card block is fixedly connected with a control strip, the outer wall of the card block is elastically connected to the inner wall of the mounting groove by spring 2, a bayonet is provided at the front end of the slide bar, the inner wall of the fixed strip is fixedly connected with a slide rod, the outer wall of the slide rod passes through and is slidably connected to the inner wall of the control strip.

[0015] As a further description of the above technical solution:

[0016] A motor is fixedly connected to the inner wall of the workbench, and an output shaft of the motor passes through the inner wall of the workbench and is fixedly connected to the bottom end of the turntable.

[0017] As a further description of the above technical solution:

[0018] The inner wall of the mounting plate and the injection cylinder, which are in the same vertical area, is provided with a hole for the injection cylinder to pass through.

[0019] As a further description of the above technical solution:

[0020] The front end of the turntable is located in the front end area of the vertical area where the workbench is located, and the vertical area where the center of gravity of the turntable is located is within the vertical area where the workbench is located.

[0021] The utility model has the following beneficial effects:

[0022] 1. In the utility model, by setting the cylinder, slide plate, syringe, sliding plug, liquid outlet pipe, one-way valve 1, liquid inlet pipe, one-way valve 2, baffle, mounting plate, air pressure chamber, slide bar, material receiving box, control block, and spring 1, it is ensured that when the syringe moves to a higher position from the culture dish, the material receiving box can be moved between the turntable and the syringe, thereby preventing the residual injection liquid on the inner wall of the syringe from dripping onto the turntable after the injection is completed.

[0023] 2. In the present invention, by setting the fixing strip, the mounting groove, the card block, the control strip, the second spring, the bayonet and the slide rod, it is ensured that after the culture medium is added to all the culture dishes, the staff can take out the receiving box from the front end, and process or reuse the culture medium in the receiving box according to the situation, thereby achieving the effect of saving resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a three-dimensional structural diagram of the overall structure of the utility model;

[0025] Figure 2 It is a schematic cross-sectional view of the three-dimensional structure of the overall structure of the present invention;

[0026] Figure 3 For this utility model Figure 2 A magnified schematic diagram of the three-dimensional structure of part A;

[0027] Figure 4 This is a schematic cross-sectional view of the three-dimensional structure of the sliding bar and the material receiving box in the utility model;

[0028] Figure 5 For this utility model Figure 4Schematic diagram of the three-dimensional structure cross section of part B;

[0029] Figure 6 It is a schematic cross-sectional view of the three-dimensional structure of the syringe in the present invention.

[0030] Legend:

[0031] 1. Workbench; 2. Mounting frame; 3. Liquid storage barrel; 4. Turntable; 5. Storage port; 6. Culture dish; 7. Filling mechanism; 8. Leakage prevention device; 9. Connecting assembly; 71. Cylinder; 72. Slide plate; 73. Syringe; 74. Sliding plug; 75. Liquid outlet pipe; 76. One-way valve 1; 77. Liquid inlet pipe; 78. One-way valve 2; 79. Baffle; 81. Mounting plate; 82. Air pressure chamber; 83. Slide bar; 84. Material receiving box; 85. Control block; 86. Spring 1; 91. Fixing bar; 92. Mounting slot; 93. Block; 94. Control bar; 95. Spring 2; 96. Bayonet; 97. Slide bar; 10. Motor. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] Reference Figure 1 and Figure 2 The utility model provides an embodiment of a cell culture fluid filling device with a leak-proof structure, comprising a workbench 1, a switch for controlling the internal circuit of the device and other devices is provided at the front end of the workbench 1, a mounting frame 2 is fixedly connected to the top of the workbench 1, and the mounting frame 2 is L-shaped. A liquid storage barrel 3 is fixedly connected to the top of the workbench 1, and a ventilation hole is provided at the top of the liquid storage barrel 3. The setting of the ventilation hole ensures that the culture fluid inside the liquid storage barrel 3 can be smoothly discharged after being sucked out, and a turntable 4 is rotatably connected to the top of the workbench 1. The top view of the turntable 4 is circular, and the front end of the turntable 4 is located in the front end area of the vertical area where the workbench 1 is located, and the vertical area where the center of gravity of the turntable 4 is located is within the vertical area where the workbench 1 is located. The position setting of the turntable 4 ensures that when the staff is at the front end of the workbench 1, they can easily take out objects from the turntable 4 and place new objects. A storage opening 5 is opened on the inner wall of the turntable 4, and a culture dish 6 is placed inside the storage opening 5. The top of the outer wall of the culture dish 6 is provided with a protrusion with a diameter larger than the storage opening 5.

[0034] Reference Figure 2 、 Figure 3 and Figure 6The inner side of the mounting frame 2 is provided with a filling mechanism 7, which includes a cylinder 71 and a slide plate 72. The cylinder 71 is fixedly connected to the top of the mounting frame 2, and the rear end of the slide plate 72 is slidably connected to the front surface of the mounting frame 2. The sliding direction of the slide plate 72 is up and down. The bottom end of the slide plate 72 is fixedly connected to the injection cylinder 73, and the inner wall piston of the injection cylinder 73 is connected to the sliding plug 74. The edge of the sliding plug 74 fits the inner wall of the injection cylinder 73, and the top of the sliding plug 74 is fixedly connected to the output end of the cylinder 71. The friction force between the sliding plug 74 and the injection cylinder 73 is greater than the sum of the friction force between the slide plate 72 and the mounting frame 2 and the elastic force of the spring 86. The output end of the cylinder 71 passes through the inner wall of the slide plate 72, and the output end of the cylinder 71 passes through the injection The inner wall of the injection cylinder 73 and the bottom end of the injection cylinder 73 are fixedly connected with a liquid outlet pipe 75, and a one-way valve 76 is provided inside the liquid outlet pipe 75. The flow direction of the one-way valve 76 is from top to bottom. The right side of the injection cylinder 73 is fixedly connected with a liquid inlet pipe 77, and a one-way valve 2 78 is provided inside the liquid inlet pipe 77. The flow direction of the one-way valve 2 78 is from right to left. The end of the liquid inlet pipe 77 away from the injection cylinder 73 passes through and is fixedly connected to the inner wall of the liquid storage barrel 3. The filling mechanism 7 also includes a baffle 79. When the injection cylinder 73 moves to a position close to the culture dish 6, the top of the baffle 79 just contacts the bottom end of the slide 72, and the rear end of the baffle 79 is fixedly connected to the front surface of the mounting frame 2 below the slide 72.

[0035] Reference Figure 2 - Figure 4 The interior of the workbench 1 is provided with a leak-proof device 8, which includes a mounting plate 81. The rear end of the mounting plate 81 is fixedly connected to the front surface of the mounting frame 2. The inner wall of the mounting plate 81 and the syringe 73 are in the same vertical area and is provided with a hole for the syringe 73 to pass through. The setting of the hole ensures that the syringe 73 will not be hindered by the mounting plate 81 during the downward movement. The inner wall of the mounting plate 81 and the inner wall of the mounting frame 2 are jointly provided with an air pressure chamber 82, and the inner wall piston of the air pressure chamber 82 is connected to a slide bar 83. The moving direction of the slide bar 83 is forward and backward, and the outer wall of the slide bar 83 is in contact with the inner wall of the air pressure chamber 82. The front end of the slide bar 83 is provided with a material receiving box 84, and the top of the material receiving box 84 is provided with a groove. The setting of the groove ensures that the dripping culture fluid can enter the interior of the material receiving box 84.

[0036] Reference Figure 3 - Figure 5The slide bar 83 is fixed to the material receiving box 84 by a connecting assembly 9. The connecting assembly 9 includes a fixing bar 91. The front and rear ends of the fixing bar 91 are both on the outside of the material receiving box 84. The outer wall of the fixing bar 91 passes through and is fixedly connected to the inner wall of the material receiving box 84. The inner wall of the fixing bar 91 is provided with a mounting groove 92. The inner wall of the mounting groove 92 is slidably connected with a card block 93. The card block 93 is in the shape of a rectangular parallelepiped. The front end of the card block 93 is fixedly connected with a control bar 94. The front end of the control bar 94 is in front of the fixing bar 91. The front end position setting of the control bar 94 ensures that the staff can easily contact the control bar 94 and adjust its position. The card block 93 The outer wall of the fixing bar 91 is elastically connected to the inner wall of the mounting groove 92 by a second spring 95. One end of the second spring 95 is fixedly connected to the outer wall of the block 93. The other end of the second spring 95 is fixedly connected to the inner wall of the mounting groove 92. A bayonet 96 is provided at the front end of the slide bar 83. The bayonet 96 is T-shaped in top view, and the rear end protruding position of the bayonet 96 matches the shape of the block 93. The inner wall of the fixing bar 91 is fixedly connected with a sliding rod 97. The setting of the sliding rod 97 ensures that the control bar 94 can move in the horizontal direction during the movement of the control bar 94 without bending. The outer wall of the sliding rod 97 passes through and is slidably connected to the inner wall of the control bar 94.

[0037] Reference Figure 2 - Figure 4 The inner wall piston of the air pressure chamber 82 is connected to the control block 85, the top of the control block 85 is elastically connected to the inner wall of the air pressure chamber 82 through a spring 86, one end of the spring 86 is fixedly connected to the top of the control block 85, and the other end of the spring 86 is fixedly connected to the inner wall of the air pressure chamber 82. The inner wall of the workbench 1 is fixedly connected to the motor 10, and the output shaft of the motor 10 passes through the inner wall of the workbench 1 and is fixedly connected to the bottom end of the turntable 4. The output shaft of the motor 10 is coaxial with the midpoint of the turntable 4.

[0038] Working principle: When in use, the staff sits at the front end of the workbench 1, takes out the culture dish 6 from the storage port 5 closest to it, puts in a new culture dish 6, and then controls the motor 10 to rotate, so that the motor 10 drives the turntable 4 to rotate, so that the storage port 5 adjacent to the storage port 5 that was previously directly below the injection cylinder 73 moves to directly below the injection cylinder 73.

[0039] When the movement is completed, the staff starts the cylinder 71, causing the output shaft of the cylinder 71 to move downward, thereby pushing the sliding plug 74 to move downward. Since the friction between the sliding plug 74 and the injection cylinder 73 is greater than the friction between the slide 72 and the mounting frame 2, the sliding plug 74, the injection cylinder 73 and the slide 72 move downward together at this time.

[0040] When the slide 72 moves downward to separate from the control block 85, the control block 85 moves downward under the elastic force of spring 1 86, thereby reducing the air pressure inside the air pressure chamber 82, causing the slide 83 to move backward under the action of the air pressure, and driving the material receiving box 84 to move backward, thereby causing the material receiving box 84 to leave the vertical position where the injection cylinder 73 is located.

[0041] When the slide 72 moves to contact the upper surface of the baffle 79, the slide 72 cannot move further downward, so at this time the sliding plug 74 moves downward under the drive of the output shaft of the cylinder 71, thereby pushing the culture fluid inside the syringe 73 into the culture dish 6.

[0042] When the injection is completed, the staff moves the output shaft of the cylinder 71 upward, so that the output shaft of the cylinder 71 drives the sliding plug 74 to move upward. Due to the friction force, the sliding plug 74, the slide plate 72 and the injection barrel 73 move upward together.

[0043] When it moves to contact with the control block 85, the slide 72 pushes the control block 85 upward, causing the control block 85 to enter the air pressure chamber 82, thereby reducing the internal space of the air pressure chamber 82, causing the slide 83 to move forward under the action of air pressure, thereby causing the material receiving box 84 to move to the vertical position where the injection cylinder 73 is located.

[0044] At this time, since the slide 72 cannot continue to move upward, when the output shaft of the cylinder 71 continues to move upward, it can drive the sliding plug 74 to move upward. Since the one-way valve 76 can only flow from top to bottom, the culture medium inside the liquid storage barrel 3 can be sucked into the syringe 73 at this time.

[0045] After completing this operation, the staff repeats the above operation until the culture medium is added to all culture dishes 6. The staff pushes the control bar 94 in the direction of approaching each other, so that the control bar 94 drives the block 93 to move in the direction of entering the installation groove 92, so that the block 93 leaves the bayonet 96.

[0046] When the block 93 leaves the latch 96 , the staff moves the fixing bar 91 forward to take out the receiving box 84 , and then the staff can further process the culture solution inside the receiving box 84 .

[0047] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cell culture fluid filling device with a leak-proof structure, comprising a workbench (1), characterized in that: The top of the workbench (1) is fixedly connected to a mounting frame (2), the top of the workbench (1) is fixedly connected to a liquid storage barrel (3), the top of the workbench (1) is rotatably connected to a turntable (4), the inner wall of the turntable (4) is provided with a storage port (5), a culture dish (6) is placed inside the storage port (5), a filling mechanism (7) is provided on the inner side of the mounting frame (2), the filling mechanism (7) comprises a cylinder (71) and a slide plate (72), the cylinder (71) is fixedly connected to the top of the mounting frame (2), the rear end of the slide plate (72) is slidably connected to the front surface of the mounting frame (2), a leak-proof device (8) is provided inside the workbench (1), the leak-proof device (8) comprises a mounting plate (81), the rear end of the mounting plate (81) is fixedly connected to the front surface of the mounting frame (2), and the inner wall of the mounting plate (81) and the inner wall of the mounting frame (2) are jointly provided with an air pressure chamber (82).

2. The cell culture fluid filling device with a leak-proof structure according to claim 1, characterized in that: The bottom end of the slide (72) is fixedly connected to a syringe (73), the inner wall piston of the syringe (73) is connected to a sliding plug (74), the top end of the sliding plug (74) is fixedly connected to the output end of the cylinder (71), the output end of the cylinder (71) passes through the inner wall of the slide (72), the output end of the cylinder (71) passes through the inner wall of the syringe (73), the bottom end of the syringe (73) is fixedly connected to a liquid outlet pipe (75), a one-way valve (76) is provided inside the liquid outlet pipe (75), the right side of the syringe (73) is fixedly connected to a liquid inlet pipe (77), a one-way valve (78) is provided inside the liquid inlet pipe (77), and the end of the liquid inlet pipe (77) away from the syringe (73) passes through and is fixedly connected to the inner wall of the liquid storage barrel (3).

3. The cell culture fluid filling device with a leak-proof structure according to claim 1, characterized in that: The filling mechanism (7) further comprises a baffle (79), the rear end of which is fixedly connected to the front surface of the mounting frame (2) below the slide plate (72).

4. The cell culture fluid filling device with a leak-proof structure according to claim 1, characterized in that: The inner wall piston of the air pressure chamber (82) is connected with a slide bar (83), and a material receiving box (84) is provided at the front end of the slide bar (83). The slide bar (83) and the material receiving box (84) are fixed by a connecting assembly (9). The connecting assembly (9) includes a fixing bar (91). The outer wall of the fixing bar (91) passes through and is fixedly connected to the inner wall of the material receiving box (84). The inner wall of the fixing bar (91) is provided with a mounting groove (92). The inner wall piston of the air pressure chamber (82) is connected with a control block (85). The top end of the control block (85) is elastically connected to the inner wall of the air pressure chamber (82) by a spring (86).

5. The cell culture fluid filling device with a leak-proof structure according to claim 4, characterized in that: The inner wall of the mounting groove (92) is slidably connected to a card block (93), the front end of the card block (93) is fixedly connected to a control strip (94), the outer wall of the card block (93) is elastically connected to the inner wall of the mounting groove (92) via a second spring (95), the front end of the slide bar (83) is provided with a bayonet (96), the inner wall of the fixed strip (91) is fixedly connected to a slide bar (97), the outer wall of the slide bar (97) passes through and is slidably connected to the inner wall of the control strip (94).

6. The cell culture fluid filling device with a leak-proof structure according to claim 1, characterized in that: A motor (10) is fixedly connected to the inner wall of the workbench (1), and an output shaft of the motor (10) passes through the inner wall of the workbench (1) and is fixedly connected to the bottom end of the turntable (4).

7. The cell culture fluid filling device with a leak-proof structure according to claim 2, characterized in that: The inner wall of the mounting plate (81) and the injection cylinder (73) in the same vertical area is provided with a hole for the injection cylinder (73) to pass through.

8. The cell culture fluid filling device with a leak-proof structure according to claim 1, characterized in that: The front end of the turntable (4) is located in the front end area of the vertical area where the workbench (1) is located, and the vertical area where the center of gravity of the turntable (4) is located is within the vertical area where the workbench (1) is located.

Images