Auxiliary device for subarachnoid space injection or sampling
By designing auxiliary devices for spinal clamping components and indwelling hoses, the damage and cost increase caused by multiple incision of mouse tissues is solved, and efficient subarachnoid sampling and injection operations are achieved.
Patent Information
- Application Number
- CN202422106776.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-28
- Publication Date
- 2025-08-15
- Estimated Expiration
- 2034-08-28
AI Technical Summary
In the prior art, the mouse tissue needs to be cut multiple times when extracting cerebrospinal fluid from the subarachnoid cavity of mice, resulting in increased experimental time and cost and causing damage to mice.
An auxiliary device including a spinal clamping assembly and an indwelling hose is designed to place the indwelling hose in the subarachnoid cavity by fixing it on the mouse spine in one go for multiple sampling and injection operations to avoid repeated incision of tissue.
Effectively prevent tissue adhesions, improve experimental efficiency, reduce mice damage, and reduce experimental costs.
Smart Images
Figure CN223220560U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of medical experiments, and more particularly to an auxiliary device for subarachnoid space injection or sampling. Background Art
[0002] Spinal cord injury is a common, severe traumatic injury to the central nervous system. Recent animal studies and clinical research have reported that subarachnoid injection of umbilical cord mesenchymal stem cells can treat spinal cord injury. This is because mesenchymal stem cells have a limited survival period and migration ability in the body. Multiple injections of umbilical cord mesenchymal stem cells into the subarachnoid space can enhance therapeutic efficacy and maintain cell numbers.
[0003] When conducting animal experiments to study spinal cord treatment methods, mesenchymal stem cells need to be injected into the mouse's subarachnoid space multiple times, and cerebrospinal fluid samples need to be extracted from the mouse's subarachnoid space multiple times. Before performing subarachnoid injections or sampling, the mouse's skin, muscles, and vertebral lamina must be cut in sequence so that the ports of the syringe and extractor can be inserted into the mouse's subarachnoid space. Because tissues have regenerative and repair functions, the incised wounds are prone to re-adhesion due to cell regeneration, so these adhered tissues need to be re-cut before each injection or sampling. On the one hand, multiple incision operations will further increase the strength of tissue adhesions, making tissue exposure more difficult. In addition, the scars caused by multiple incisions and repairs will also adhere to the mouse's spinal cord, causing greater damage to the mouse. On the other hand, multiple incision operations will increase experimental time and cost. Utility Model Content
[0004] In response to the problem in the above-mentioned prior art that multiple incisions are required in the mouse tissue when extracting cerebrospinal fluid or injecting drugs from the mouse's subarachnoid space, which increases experimental time and cost and causes damage to the mouse, the utility model provides an auxiliary device for subarachnoid space injection or sampling, which can help experimenters perform multiple sampling and injection operations in the mouse's subarachnoid space with only one tissue incision.
[0005] In order to solve the above technical problems, the technical solution provided by the present invention is:
[0006] An auxiliary device for subarachnoid injection or sampling includes a spinal clamping assembly and a retention hose detachably connected to the spinal clamping assembly, one end of the retention hose is used to extend into the subarachnoid space of a mouse, and the spinal clamping assembly is provided with a tube through-hole for allowing the retention hose to pass through.
[0007] In the above technical solution, after the spinal cord of the mouse is exposed during the first spinal cord injury modeling, one end of the indwelling hose is passed through the tube-piercing hole and extended into the subarachnoid space of the mouse, thereby retaining one end of the indwelling hose in the subarachnoid space; then the spinal clamping assembly is fixed to the mouse spine, and during the operation, the spinal clamping assembly is prevented from pulling the fixed end of the indwelling hose (i.e., the end inserted into the subarachnoid space of the mouse); at this time, the spinal clamping assembly and the mouse spine remain relatively fixed, and the indwelling hose is also kept stable under the limiting effect of the tube-piercing hole. When it is necessary to extract cerebrospinal fluid from the subarachnoid space of the mouse or to inject cells or drugs into the subarachnoid space of the mouse, the free end of the indwelling hose (i.e., the end away from the subarachnoid space of the mouse) can be used for extraction or injection, and the cerebrospinal fluid in the subarachnoid space of the mouse can be extracted or the injection can be delivered to the subarachnoid space of the mouse through the indwelling hose. On the one hand, the above operation places the device above the exposed spinal cord of the mouse, thereby effectively preventing the occurrence of tissue adhesion; on the other hand, when performing injection or sampling operations again, it can be done directly through the indwelling hose, without having to cut the tissue again to expose the spinal cord, avoiding repeated cutting of the injured area.
[0008] In one preferred embodiment, the spinal clamping assembly includes a left clamping member, a right clamping member and a top plate, wherein the left clamping member and the right clamping member are located on one side of the top plate and are detachably connected to the top plate respectively, and a clamping cavity for accommodating the mouse spine is formed between the left clamping member, the top plate and the right clamping member; and the through-tube hole is provided on the top plate. During implementation, the top plate is first abutted against the spinal lamina of the mouse, and then the left clamping member and the right clamping member are respectively fixed on the top plate, so that the left clamping member and the right clamping member are respectively abutted against the transverse processes on both sides of the mouse spine, thereby fixing the spinal clamping assembly on the mouse spine. Of course, the spinal clamping assembly can also be a clamping structure such as an elastic gripping clamp assembly, but such a structure is not easy to operate and is prone to clamping the mouse spine.
[0009] Preferably, the device further comprises a first fastening screw and a second fastening screw, wherein the left clamping member is provided with a first threaded hole, the right clamping member is provided with a second threaded hole, and the top plate is provided with a first guide hole and a second guide hole; one end of the first fastening screw passes through the first guide hole and is threadedly connected to the first threaded hole, and one end of the second fastening screw passes through the second guide hole and is threadedly connected to the second threaded hole; the first fastening screw and the first guide hole are slidably arranged along the width direction of the clamping cavity, and the second fastening screw and the second guide hole are slidably arranged along the width direction of the clamping cavity. It is understandable that the width direction of the clamping cavity is on the line connecting the left clamping member and the right clamping member. During implementation, the spacing between the left and right clamps can be adjusted so that the left and right clamps respectively contact the transverse processes on both sides of the mouse spine. The positions of the first fastening screw and the second fastening screw on the first guide hole and the second guide hole are then adjusted so that the first fastening screw and the second fastening screw are aligned with the first threaded hole and the second threaded hole, respectively. Then, one end of the first fastening screw and one end of the second fastening screw are screwed into the first threaded hole and the second threaded hole, respectively, until the left and right clamps remain relatively fixed to the top plate. In other words, the spacing between the left and right clamps is adjustable. Adjusting the spacing between the left and right clamps ensures that the left and right clamps are always clamped on the transverse processes on both sides of the mouse spine, making the device suitable for different mice.
[0010] Preferably, the left clamping member is provided with a first arcuate groove on a side proximal to the right clamping member; the right clamping member is provided with a second arcuate groove on a side proximal to the left clamping member; and the clamping cavity is formed between the first arcuate groove, the second arcuate groove, and the top plate. The surfaces of the transverse processes on both sides of the mouse spine that contact the left and right clamping members are both arcuate surfaces. The first and second arcuate grooves are provided to abut the transverse processes on both sides of the mouse spine, respectively, allowing the left and right clamping members to better fit the transverse processes of the mouse spine, thereby making the connection between the spinal clamping assembly and the mouse spine more secure and stable.
[0011] Preferably, the top plate is provided with a perspective structure, through which the experimenter can see the position of one end of the indwelling hose, thereby placing the one end of the indwelling hose in a more accurate position to ensure smooth sampling and injection.
[0012] Preferably, the perspective structure is a transparent glass structure, and the tube through hole is provided on the perspective structure. Transparent glass material can provide a clearer field of view than transparent colorless materials such as plastic, silicone or resin, and helps the experimenter to more accurately place one end of the indwelling hose in the correct position.
[0013] Among them, the top plate can be made of a transparent glass structure as a whole, but glass is fragile, so the top plate is made of a material different from transparent glass, such as stainless steel or other materials with higher strength than glass, so as to ensure the durability of the entire device.
[0014] Preferably, the top plate is provided with a mounting groove, the inner wall of the mounting groove being provided with a sealing resin layer, and the see-through structure is located within the mounting groove and connected to the inner wall of the mounting groove via the sealing resin layer. The provision of the sealing resin layer adheres the see-through structure to the mounting groove, thereby ensuring the firmness of the see-through structure.
[0015] Preferably, a puncture needle is provided at one end of the indwelling hose, the inner lumen of the puncture needle being connected to the inner lumen of the indwelling hose, and the puncture needle is used to be inserted into the subarachnoid space of the mouse. It should be noted that the puncture needle is a prior art and can be used for injecting or extracting liquids. The small diameter of the puncture needle can avoid damage to other structures in the subarachnoid space, while reducing pressure and unnecessary stimulation on the subarachnoid space during operation. In addition, the puncture needle is easier to control, which helps to more accurately obtain cerebrospinal fluid samples or inject drugs.
[0016] Preferably, the end of the indwelling hose distal from the puncture needle is provided with a positioning structure. The positioning structure includes a positioning cavity that communicates with the inner lumen of the indwelling hose, and the cross-sectional area of the positioning cavity is larger than the cross-sectional area of the inner lumen of the indwelling hose. It should be noted that the cross-sectional area of the inner lumen of the indwelling hose refers to a plane perpendicular to the axis of the indwelling hose, and the cross-sectional area of the positioning cavity is parallel to this plane. The provision of the positioning cavity facilitates the rapid positioning of an external syringe and extractor at the port of the indwelling hose.
[0017] Preferably, a sealing rubber plug is detachably connected to the positioning structure, and the sealing rubber plug is used to seal the positioning cavity. In the initial state, the sealing rubber plug seals the positioning cavity to prevent cerebrospinal fluid from flowing out through the positioning cavity and to prevent foreign objects from entering the inner cavity of the indwelling hose and contaminating it. After confirming the sampling or injection position of the indwelling hose and confirming that the left clamp and the right clamp clamp the transverse process of the mouse spine, remove the sealing rubber plug and start the sampling or injection operation. After the operation is completed, the sealing rubber plug is used to seal the positioning cavity.
[0018] Beneficial effects of the utility model:
[0019] (1) By setting up a spinal clamping assembly and a retention hose, the spinal clamping assembly is fixed on the mouse spine during use, and one end of the retention hose is placed in the subarachnoid space of the mouse. The subarachnoid space of the mouse is sampled and injected through the retention hose. On the one hand, the device can be left above the exposed spinal cord of the mouse, which can effectively prevent the occurrence of tissue adhesion; on the other hand, when performing the injection or sampling operation again, it can be performed directly through the retention hose without cutting the tissue again to expose the spinal cord. This not only improves the experimental efficiency, but also avoids further damage to the mouse caused by multiple cuts at the injured area.
[0020] (2) The spine clamping assembly includes a top plate and left and right clamping members with adjustable spacing. By adjusting the spacing between the left and right clamping members, the spine clamping assembly can be matched with mouse spines of different sizes, making the device suitable for different mice.
[0021] (3) By setting up a perspective structure, the experimenter can conveniently place one end of the indwelling hose in a more accurate position to ensure smooth sampling and injection. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is a structural schematic diagram of an indwelling hose;
[0023] Figure 2 is a schematic diagram of the structure of the spinal clamping assembly;
[0024] Figure 3 is a top view schematic diagram of the spinal clamping assembly;
[0025] Figure 4 is a schematic diagram of the left clamping member and the right clamping member in use;
[0026] Figure 5 is a schematic diagram of the spinal clamping assembly in use.
[0027] In the accompanying drawings: 1-indwelling hose; 2-left clamping piece; 201-first arcuate groove; 3-right clamping piece; 301-second arcuate groove; 4-top plate; 401-tube through hole; 402-perspective structure; 403-mounting groove; 404-first guide hole; 405-second guide hole; 5-first fastening screw; 6-second fastening screw; 7-puncture needle; 8-positioning structure; 9-sealing plug; 10-spine; 1001-vertebral plate; 1002-transverse process. DETAILED DESCRIPTION
[0028] The drawings are for illustrative purposes only and should not be construed as limiting this patent. To better illustrate the embodiments, some components in the drawings may be omitted, enlarged, or reduced in size, and do not represent actual product dimensions. Those skilled in the art will understand that some well-known structures and their descriptions may be omitted from the drawings. The positional relationships depicted in the drawings are for illustrative purposes only and should not be construed as limiting this patent.
[0029] The same or similar numbers in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are terms such as "upper", "lower", "left", "right", "long", "short", etc. indicating directions or positional relationships, they are based on the directions or positional relationships shown in the drawings. This is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and cannot be understood as limiting this patent. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.
[0030] The technical solution of the present invention is further described in detail below through specific embodiments and in conjunction with the accompanying drawings:
[0031] Example 1
[0032] like Figure 1 and Figure 2 The device, shown here, is an auxiliary device for subarachnoid injection or sampling. It includes a spinal clamping assembly and an indwelling hose 1 detachably connected to the spinal clamping assembly. One end of the indwelling hose 1 is configured to extend into the subarachnoid space of a mouse. The spinal clamping assembly is provided with a tube-penetrating hole 401 for passage of the indwelling hose 1. Specifically, when the indwelling hose 1 passes through the tube-penetrating hole 401, the outer circumferential surface of the indwelling hose 1 abuts against the inner wall of the tube-penetrating hole 401, allowing the indwelling hose 1 to slide along the axis of the tube-penetrating hole 401. The tube-penetrating hole 401 also restricts radial movement of the indwelling hose 1.
[0033] The working principle or workflow of this embodiment is as follows: after the spinal cord of the mouse is exposed by the first spinal cord injury modeling, one end of the indwelling hose 1 is passed through the tube-penetrating hole 401 and extended into the subarachnoid space of the mouse, thereby retaining one end of the indwelling hose 1 in the subarachnoid space. At this time, the two ends of the indwelling hose 1 are respectively located on the upper and lower sides of the tube-penetrating hole 401; then the spinal clamping assembly is fixed on the spine 10 of the mouse, and during the operation, the spinal clamping assembly is avoided as much as possible from pulling the fixed end of the indwelling hose 1 (i.e., the end inserted into the subarachnoid space of the mouse); at this time, the spinal clamping assembly and the spine 10 of the mouse remain relatively fixed, and the indwelling hose 4 is also kept stable under the limiting action of the tube-penetrating hole 401. When it is necessary to extract cerebrospinal fluid from the subarachnoid space of the mouse or to inject cells or drugs into the subarachnoid space of the mouse, the extraction or injection operation can be performed on the free end of the indwelling hose 1 (i.e., the end away from the subarachnoid space of the mouse), and the cerebrospinal fluid in the subarachnoid space of the mouse can be extracted or the injection can be delivered to the subarachnoid space of the mouse through the indwelling hose 1.
[0034] The beneficial effects of this embodiment are as follows: by providing a spinal clamping assembly and an indwelling hose, the spinal clamping assembly is fixed to the mouse spine during use, and one end of the indwelling hose is placed in the subarachnoid space of the mouse, and sampling and injection operations are performed on the subarachnoid space of the mouse through the indwelling hose. On the one hand, the device can be left above the exposed spinal cord of the mouse, thereby effectively preventing the occurrence of tissue adhesion; on the other hand, when performing an injection or sampling operation again, it can be performed directly through the indwelling hose without having to cut the tissue again to expose the spinal cord, which not only improves the experimental efficiency, but also avoids further damage to the mouse caused by multiple incisions at the injured area.
[0035] Example 2
[0036] This embodiment is based on embodiment 1. Figures 2 to 5 As shown (where Figure 4 and Figure 5 The arrow in the figure indicates the direction of force application), the spinal clamping assembly includes a left clamping member 2, a right clamping member 3, and a top plate 4. The left clamping member 2 and the right clamping member 3 are located on one side of the top plate 4 and are detachably connected to the top plate 4. A clamping cavity for accommodating the mouse spine 10 is formed between the left clamping member 2, the top plate 4, and the right clamping member 3. A through hole 401 for passing a tube is provided on the top plate 4. During implementation, the top plate 4 is first brought into contact with the spinal lamina 1001 of the mouse, and then the left clamping member 2 and the right clamping member 3 are respectively fixed to the top plate 4, so that the left clamping member 2 and the right clamping member 3 are respectively brought into contact with the transverse processes 1002 on both sides of the mouse spine 10, thereby fixing the spinal clamping assembly to the mouse spine 10.
[0037] Furthermore, it also includes two first fastening screws 5 and two second fastening screws 6. The left clamping member 2 is provided with two first threaded holes (not shown in the figure), the right clamping member 3 is provided with two second threaded holes (not shown in the figure), and the top plate 4 is provided with two first guide holes 404 and two second guide holes 405. One first fastening screw 5 is inserted into one first guide hole 404 and one first threaded hole, and one second fastening screw 6 is inserted into one second guide hole 405 and one second threaded hole. One end of the first fastening screw 5 passes through the first guide hole 404 and is threadedly connected to the first threaded hole, and one end of the second fastening screw 6 passes through the second guide hole 405 and is threadedly connected to the second threaded hole. The first fastening screw 5 and the first guide hole 404 are slidably arranged along the width direction of the clamping cavity, and the second fastening screw 6 and the second guide hole 405 are slidably arranged along the width direction of the clamping cavity. It can be understood that the width direction of the clamping cavity is on the line connecting the left clamping member 2 and the right clamping member 3. During implementation, the spacing between the left clamp 2 and the right clamp 3 can be adjusted so that the left clamp 2 and the right clamp 3 respectively abut against the transverse processes 1002 on both sides of the mouse spine 10, and then the positions of the first fastening screw 6 on the first guide hole 404 and the second guide hole 405 are adjusted respectively. After the first fastening screw 5 and the second fastening screw 6 are aligned with the first threaded hole and the second threaded hole, one end of the first fastening screw 5 and one end of the second fastening screw 6 are screwed into the first threaded hole and the second threaded hole respectively until the left clamp 2 and the right clamp 3 are relatively fixed to the top plate 4. In other words, the spacing between the left clamp 2 and the right clamp 3 is adjustable. By adjusting the spacing between the left clamp 2 and the right clamp 3, it can be ensured that the left clamp 2 and the right clamp 3 can always be clamped on the transverse processes 1002 on both sides of the mouse spine 10, making the device suitable for different mice.
[0038] Furthermore, a first arcuate groove 201 is provided on the side of the left clamping member 2 proximal to the right clamping member 3; a second arcuate groove 301 is provided on the side of the right clamping member 3 proximal to the left clamping member 2; and a clamping cavity is formed between the first arcuate groove 201, the second arcuate groove 301, and the top plate 4. The surfaces where the transverse processes 1002 on either side of the mouse spine 10 contact the left clamping member 2 and the right clamping member 3 are both arcuate surfaces. The provision of the first arcuate groove 201 and the second arcuate groove 301, respectively, abuts against the transverse processes 1002 on either side of the mouse spine 10, allowing the left clamping member 2 and the right clamping member 3 to fit more closely with the transverse processes 1002 of the mouse spine 10, thereby making the connection between the spinal clamping assembly and the mouse spine 10 more secure and stable.
[0039] Furthermore, a perspective structure 402 is provided on the top plate 4. The experimenter can see the position of one end of the indwelling hose 1 through the perspective structure 402, thereby placing the one end of the indwelling hose 1 in a more accurate position to ensure smooth sampling and injection.
[0040] Specifically, the perspective structure 402 is a transparent glass structure, and the tube through hole 401 is provided on the perspective structure 402. Transparent glass material provides a clearer field of view than transparent colorless materials such as plastic, silicone or resin, helping the experimenter to more accurately place one end of the indwelling hose 1 in the correct position.
[0041] Furthermore, the top plate 4 is provided with a mounting groove 403, the inner wall of which is provided with a sealing resin layer (not shown). The perspective structure 402 is located within the mounting groove 403 and is connected to the inner wall of the mounting groove 403 via the sealing resin layer. The provision of the sealing resin layer bonds the perspective structure 402 within the mounting groove 403, thereby ensuring the firmness of the perspective structure 402.
[0042] Other features, working principles and beneficial effects of this embodiment are consistent with those of embodiment 1.
[0043] Example 3
[0044] This embodiment is based on embodiment 2. Figure 1 As shown, a puncture needle 7 is provided at one end of the indwelling tube 1. The inner lumen of the puncture needle 7 is connected to the inner lumen of the indwelling tube 1 and is used to insert the puncture needle 7 into the subarachnoid space of the mouse. It should be noted that the puncture needle 7 is conventional and can be used for injecting or withdrawing fluids. The small diameter of the puncture needle 7 avoids damage to other structures within the subarachnoid space and reduces pressure and unnecessary stimulation during operation. Furthermore, the puncture needle 7 is easier to control, facilitating more accurate acquisition of cerebrospinal fluid samples or drug injection.
[0045] Furthermore, a positioning structure 8 is provided on the end of the indwelling hose 1 away from the puncture needle 7. This positioning structure 8 includes a positioning cavity (not shown) that communicates with the inner lumen of the indwelling hose 1. The cross-sectional area of the positioning cavity is larger than the cross-sectional area of the inner lumen of the indwelling hose 1. It should be noted that the cross-sectional area of the inner lumen of the indwelling hose 1 refers to a plane perpendicular to the axis of the indwelling hose 1, and the cross-sectional area of the positioning cavity is parallel to this plane. The provision of the positioning cavity facilitates the rapid positioning of external syringes and extractors at the end of the indwelling hose 1.
[0046] Furthermore, a sealing plug 9 is detachably connected to the positioning structure 8, and the sealing plug 9 is used to seal the positioning cavity. In the initial state, the sealing plug 9 seals the positioning cavity to prevent cerebrospinal fluid from flowing out through the positioning cavity and to prevent foreign objects from entering the inner cavity of the indwelling hose 1 and contaminating it. After confirming the sampling or injection position of the indwelling hose 1 and confirming that the left clamp 2 and the right clamp 3 clamp the transverse process 1002 of the mouse spine 10, remove the sealing plug 9 and start the sampling or injection operation. After the operation is completed, the sealing plug 9 is used to seal the positioning cavity. Figure 5 shown.
[0047] Other features, working principles and beneficial effects of this embodiment are consistent with those of Example 2.
[0048] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description, and it is not necessary and impossible to provide an exhaustive list of all implementation methods. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.
Claims
1. An auxiliary device for subarachnoid injection or sampling, characterized in that: The invention comprises a spinal clamping assembly and a retention hose (1) detachably connected to the spinal clamping assembly, wherein one end of the retention hose (1) is used to extend into the subarachnoid space of a mouse; the spinal clamping assembly is provided with a tube-penetrating hole (401), and the tube-penetrating hole (401) is used for allowing the retention hose (1) to pass through.
2. The auxiliary device for subarachnoid injection or sampling according to claim 1, characterized in that: The spinal column clamping assembly comprises a left clamping member (2), a right clamping member (3) and a top plate (4); the left clamping member (2) and the right clamping member (3) are located on one side of the top plate (4) and are detachably connected to the top plate (4), respectively; a clamping cavity for accommodating the mouse spine is formed between the left clamping member (2), the top plate (4) and the right clamping member (3); and the through-hole (401) is provided on the top plate (4).
3. The auxiliary device for subarachnoid injection or sampling according to claim 2, characterized in that: The invention also includes a first fastening screw (5) and a second fastening screw (6), wherein the left clamping member (2) is provided with a first threaded hole, the right clamping member (3) is provided with a second threaded hole, and the top plate (4) is provided with a first guide hole (404) and a second guide hole (405), one end of the first fastening screw (5) passes through the first guide hole (404) and is threadedly connected to the first threaded hole, and one end of the second fastening screw (6) passes through the second guide hole (405) and is threadedly connected to the second threaded hole; the first fastening screw (5) and the first guide hole (404) are slidably arranged along the width direction of the clamping cavity, and the second fastening screw (6) and the second guide hole (405) are slidably arranged along the width direction of the clamping cavity.
4. The auxiliary device for subarachnoid injection or sampling according to claim 2, characterized in that: A first arc-shaped groove (201) is provided on a side of the left clamping member (2) close to the right clamping member (3); a second arc-shaped groove (301) is provided on a side of the right clamping member (3) close to the left clamping member (2); and the clamping cavity is formed between the first arc-shaped groove (201), the second arc-shaped groove (301) and the top plate (4).
5. The auxiliary device for subarachnoid injection or sampling according to claim 2, characterized in that: A perspective structure (402) is provided on the top plate (4).
6. The auxiliary device for subarachnoid injection or sampling according to claim 5, characterized in that: The perspective structure (402) adopts a transparent glass structure, and the through-tube hole (401) is arranged on the perspective structure (402).
7. The auxiliary device for subarachnoid injection or sampling according to claim 5, characterized in that: The top plate (4) is provided with a mounting groove (403), an inner wall of the mounting groove (403) is provided with a sealing resin layer, and the perspective structure (402) is located in the mounting groove (403) and is connected to the inner wall of the mounting groove (403) through the sealing resin layer.
8. An auxiliary device for subarachnoid space injection or sampling according to any one of claims 1 to 7, characterized in that: A puncture needle (7) is provided at one end of the indwelling hose (1), the inner cavity of the puncture needle (7) is communicated with the inner cavity of the indwelling hose (1), and the puncture needle (7) is used to be inserted into the subarachnoid space of a mouse.
9. The auxiliary device for subarachnoid injection or sampling according to claim 8, characterized in that: A positioning structure (8) is provided at one end of the indwelling hose (1) away from the puncture needle (7), and a positioning cavity is provided on the positioning structure (8) and is connected to the inner cavity of the indwelling hose (1), and the cross-sectional area of the positioning cavity is larger than the cross-sectional area of the inner cavity of the indwelling hose (1).
10. The auxiliary device for subarachnoid injection or sampling according to claim 9, characterized in that: A sealing rubber plug (9) is detachably connected to the positioning structure (8).