Special culture dish for cell scratch experiment

Abstract

The utility model discloses a culture dish special for a cell scratch experiment, which relates to the technical field of cell scratch and particularly comprises a culture dish body, a supporting plate is fixedly connected to the outer side of the culture dish body, a limiting shell is fixedly connected to the top of the supporting plate, a plurality of limiting grooves are formed in the top of the limiting shell, and a movable frame is movably connected to the top of the limiting shell. And two positioning rods are fixedly connected between the inner walls of the two sides of the movable frame. Through the arrangement of the movable frame, the positioning rod, the moving hole, the moving block, the rotating rod, the lifting rod and the scratching plate, the device has the effects that when cells in a culture dish are scratched, scratching lines can form a straight line, and the scratching strength and the scratching length are unified, and the problems that when the cells are scratched, the scratching length is inconvenient to determine, and the scratching efficiency is high are effectively solved. And during scratching, scratching lines are not straight enough, the scratching force is not uniform, and the accuracy of a scratching experiment is influenced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell scratching, in particular to a special culture dish for cell scratching experiments. Background Art

[0002] The cell scratch assay is an in vitro method for studying cell migration and tumor invasion. The principle is that when cells grow to a confluent monolayer, a blank area, called a "scratch," is artificially created on the confluent monolayer. Cells at the edge of the scratch gradually move into the blank area, causing the "scratch" to heal.

[0003] Most existing traditional cell culture dishes do not have a separate scale for cell scratching, which makes it inconvenient to determine the length of the scratch when scratching cells. In addition, the scratch line is often not straight enough and the scratching force is inconsistent, affecting the accuracy of the scratch experiment.

[0004] A cell culture dish that facilitates cell scratching, disclosed in the Chinese utility model patent application publication CN201820180070.3, comprises a dish body, a receiving groove provided on the inner wall of the dish body, a glass plate provided inside the receiving groove, a transverse groove and a longitudinal groove provided at the bottom end of the dish body, an elastic pad provided at the bottom of the dish body, and an anti-slip protrusion provided at the bottom of the elastic pad. Although the cell culture dish that facilitates cell scratching has a better scratching effect, a simple structure, and a reasonable design, the cell culture dish that facilitates cell scratching has the disadvantage that the scratching can only be performed at the same position on the dish, and it is inconvenient to adjust the depth and width of the scratch according to the needs during the scratching. Utility Model Content

[0005] (1) Technical problems solved

[0006] In view of the deficiencies in the prior art, the present invention provides a special culture dish for cell scratch experiments, which solves the problems raised in the above-mentioned background technology.

[0007] (2) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: it includes a culture dish body, the outer side of the culture dish body is fixedly connected to a support plate, the top of the support plate is fixedly connected to a limiting shell, the top of the limiting shell is provided with a plurality of limiting grooves, the top of the limiting shell is movably connected to a movable frame, two positioning rods are fixedly connected between the inner walls on both sides of the movable frame, the top of the movable frame is slidably connected to a moving block, the internal rotation of the moving block is connected to a rotating rod, the internal sliding connection of the rotating rod is connected to a lifting rod, and the bottom end of the rotating rod is fixedly connected to a scratch plate.

[0009] Optionally, the outer side of the limiting shell is movably connected to a culture dish cover, and the top of the culture dish cover is fixedly connected to a plurality of fixing plates.

[0010] Optionally, a plurality of positioning grooves matching the limiting grooves are formed at the bottom of the positioning rod, and a groove matching the positioning grooves is formed at the top of the fixing plate.

[0011] Optionally, a movable hole is opened inside the movable frame, the rotating rod is located inside the movable hole, and a scale line is provided on the top of the movable frame.

[0012] Optionally, a positioning plate is slidably connected to the outer side of the rotating rod, an adjusting column is fixedly connected to the top of the moving block, and a plurality of adjusting holes adapted to the adjusting columns are opened inside the positioning plate.

[0013] Optionally, an outer side of the rotating rod is fixedly connected to an adjusting shell, and an movable hole is opened on the outer side of the adjusting shell.

[0014] Optionally, the movable hole is in an E-shape, and the outer side of the rotating rod is slidably connected to a limiting rod that is compatible with the movable hole.

[0015] (3) Beneficial effects

[0016] The utility model provides a special culture dish for cell scratch experiment, which has the following beneficial effects:

[0017] 1. The culture dish for cell scratch experiments, through the arrangement of a movable frame, a positioning rod, a movable hole, a movable block, a rotating rod, a lifting rod and a scratching plate, enables the device to form a straight scratch line and uniform scratch force and length when scratching cells in the culture dish, effectively solving the problem of inconvenience in determining the scratch length when scratching cells, and the scratch line is often not straight enough and the scratch force is inconsistent, which affects the accuracy of the scratch experiment.

[0018] 2. The culture dish specially used for cell scratching experiments, through the arrangement of the limiting shell, limiting groove, positioning groove, rotating rod, lifting rod, positioning plate, adjusting column, adjusting hole, movable hole, adjusting shell, movable hole and limiting rod, enables the device to adjust the height and position of the scratch plate and the width of the scratch on the scratch plate according to the needs of cell scratching, thereby playing the role of scratching cells with different depths and widths, effectively solving the problem that scratching cells can only be performed at the same position of the culture dish, and it is inconvenient to adjust the scratch depth and width according to the needs during scratching. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic structural diagram of the axial view of the culture dish body of the present invention;

[0020] Figure 2 This is a schematic diagram of the structure of the utility model from an axial perspective;

[0021] Figure 3 This is a schematic structural diagram of the axial view of the movable frame of the utility model;

[0022] Figure 4 This is a schematic structural diagram of the movable frame of the utility model when viewed from the upper axis;

[0023] Figure 5 For this utility model Figure 3 A schematic diagram of the structure enlarged at point A;

[0024] Figure 6 This is a schematic structural diagram of the interior view of the culture dish of the present invention.

[0025] In the figure: 1. Culture dish body; 2. Support plate; 3. Limiting shell; 4. Culture dish cover; 5. Limiting groove; 6. Fixed plate; 7. Movable frame; 8. Positioning rod; 9. Positioning groove; 10. Moving block; 11. Rotating rod; 12. Lifting rod; 13. Positioning plate; 14. Adjusting column; 15. Adjusting hole; 16. Moving hole; 17. Adjusting shell; 18. Movable hole; 19. Limiting rod; 20. Scratch plate. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0027] Example 1

[0028] See also Figures 1 to 6The utility model provides a technical solution: a special culture dish for cell scratch experiments, including a culture dish body 1, a support plate 2 is fixedly connected to the outside of the culture dish body 1, a limit shell 3 is fixedly connected to the top of the support plate 2, a plurality of limit slots 5 are provided on the top of the limit shell 3, a movable frame 7 is movably connected to the top of the limit shell 3, a movable hole 16 is provided inside the movable frame 7, a rotating rod 11 is located inside the movable hole 16, a scale line is provided on the top of the movable frame 7, two positioning rods 8 are fixedly connected between the inner walls on both sides of the movable frame 7, a movable block 10 is slidably connected to the top of the movable frame 7, the inner part of the movable block 10 is rotatably connected to the rotating rod 11, and the inner sliding part of the rotating rod 11 It is movably connected with a lifting rod 12, the bottom end of the rotating rod 11 is fixedly connected with a scratching plate 20, the outer side of the limiting shell 3 is movably connected with a culture dish cover 4, and the top of the culture dish cover 4 is fixedly connected with multiple fixed plates 6. Through the arrangement of the movable frame 7, the positioning rod 8, the moving hole 16, the moving block 10, the rotating rod 11, the lifting rod 12 and the scratching plate 20, the device has the effect of making the scratch line form a straight line and making the scratch force and scratch length uniform when scratching the cells in the culture dish, effectively solving the problem that it is inconvenient to determine the scratch length when scratching the cells, and the scratch line is often not straight enough and the scratch force is different, which affects the accuracy of the scratch experiment.

[0029] When in use, the culture dish cover 4 is opened and the positioning rod 8 on the top of the fixed plate 6 is removed so that the movable frame 7 passes through the culture dish cover 4, and then the positioning rod 8 is placed on the top of the limiting shell 3, after which the scratch plate 20 contacts the cells in the culture dish body 1, and then the scale line running through the top of the movable frame 7 moves the position of the movable block 10 so that the rotating rod 11 drives the lifting rod 12 to move, thereby moving the scratch plate 20 to scratch the cells, effectively solving the problem of being inconvenient to determine the length of the scratch when scratching the cells, and the scratch line is often not straight enough and the scratching force is uneven, which affects the accuracy of the scratch experiment.

[0030] Example 2

[0031] See also Figures 4 to 6The utility model provides a technical solution: a special culture dish for cell scratch experiment, the bottom of the positioning rod 8 is provided with a plurality of positioning grooves 9 adapted to the limiting grooves 5, the top of the fixing plate 6 is provided with a groove adapted to the positioning groove 9, the outer side of the rotating rod 11 is slidably connected with a positioning plate 13, the top of the moving block 10 is fixedly connected with an adjusting column 14, the interior of the positioning plate 13 is provided with a plurality of adjusting holes 15 adapted to the adjusting column 14, the outer side of the rotating rod 11 is fixedly connected with an adjusting shell 17, the outer side of the adjusting shell 17 is provided with a movable hole 18, the shape of the movable hole 18 is E-shaped, the outer side of the rotating rod 11 is slidably connected with a movable hole 18 adapted to the movable hole 18 The limiting rod 19 is arranged through the limiting shell 3, the limiting groove 5, the positioning groove 9, the rotating rod 11, the lifting rod 12, the positioning plate 13, the adjusting column 14, the adjusting hole 15, the moving hole 16, the adjusting shell 17, the movable hole 18 and the limiting rod 19, so that the device has the effect of adjusting the height and position of the scratching plate 20 and the width of the scratching of the scratching plate 20 according to the needs of cell scratching, thereby playing the role of scratching cells with different depths and widths, effectively solving the problem that when scratching cells, the scratching can only be performed at the same position of the culture dish, and it is inconvenient to adjust the depth and width of the scratch according to the needs during scratching.

[0032] When in use, the scratch plate 20 is adjusted according to needs. During adjustment, the two positioning rods 8 are located inside different limit grooves 5, thereby adjusting the position of the scratch plate 20 inside the culture dish body 1, and then the positioning disk 13 is pulled to disengage the adjustment hole 15 from the adjustment column 14, and then the rotating rod 11 is rotated to adjust the angle of the scratch plate 20, thereby adjusting the width of the cell scratches made by the device, and then the positioning disk 13 is lowered to allow the adjustment column 14 to enter the other adjustment holes 15 to fix the angle of the scratch plate 20, and then the limiting rod 19 is slid so that the limiting rod 19 is located in the vertical hole of the movable hole 18, and then the height of the lifting rod 12 is adjusted to adjust the scratch depth of the scratch plate 20, and then the limiting rod 19 is slid again so that the limiting rod 19 enters the remaining horizontal holes of the movable hole 18 to fix the height of the scratch plate 20, which effectively solves the problem that cells can only be scratched at the same position of the culture dish when scratching, and it is inconvenient to adjust the depth and width of the scratch according to needs during scratching.

[0033] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A special culture dish for cell scratch experiment, comprising a culture dish body (1), characterized in that: The outer side of the culture dish body (1) is fixedly connected to a support plate (2), the top of the support plate (2) is fixedly connected to a limit shell (3), the top of the limit shell (3) is provided with a plurality of limit slots (5), the top of the limit shell (3) is movably connected to a movable frame (7), two positioning rods (8) are fixedly connected between the inner walls of both sides of the movable frame (7), the top of the movable frame (7) is slidably connected to a moving block (10), the interior of the moving block (10) is rotatably connected to a rotating rod (11), the interior of the rotating rod (11) is slidably connected to a lifting rod (12), and the bottom end of the rotating rod (11) is fixedly connected to a scratch plate (20).

2. The culture dish for cell scratch test according to claim 1, characterized in that: The outer side of the limiting shell (3) is movably connected to a culture dish cover (4), and the top of the culture dish cover (4) is fixedly connected to a plurality of fixing plates (6).

3. The culture dish for cell scratch test according to claim 2, characterized in that: The bottom of the positioning rod (8) is provided with a plurality of positioning grooves (9) that are matched with the limiting grooves (5), and the top of the fixing plate (6) is provided with a groove that is matched with the positioning grooves (9).

4. The culture dish for cell scratch test according to claim 1, characterized in that: A movable hole (16) is provided inside the movable frame (7), the rotating rod (11) is located inside the movable hole (16), and a scale line is provided on the top of the movable frame (7).

5. The culture dish for cell scratch test according to claim 4, characterized in that: The outer side of the rotating rod (11) is slidably connected to a positioning plate (13), the top of the moving block (10) is fixedly connected to an adjustment column (14), and the interior of the positioning plate (13) is provided with a plurality of adjustment holes (15) adapted to the adjustment columns (14).

6. The culture dish for cell scratch test according to claim 1, characterized in that: The outer side of the rotating rod (11) is fixedly connected to an adjusting shell (17), and a movable hole (18) is provided on the outer side of the adjusting shell (17).

7. The culture dish for cell scratch test according to claim 6, characterized in that: The movable hole (18) is in an E-shape, and a limiting rod (19) adapted to the movable hole (18) is slidably connected to the outer side of the rotating rod (11).

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