Biological scientific cell staining device

Abstract

The utility model is applicable to the technical field of cell staining devices, and provides a bioscience cell staining device which comprises a plurality of placing seats, a driving mechanism, a staining unit and a positioning structure, the driving mechanism comprises a bottom plate, a first stepping motor, a mounting disc and a sleeve, and the positioning structure comprises a driving rod, a positioning plate, an inflation and deflation pump and an elastic rope. The bioscience cell staining device is provided with a plurality of placing seats, a driving mechanism, a staining unit and a positioning structure, the placing seats are driven by a driving machine to move to staining stations one by one, and the staining unit comprises an injection cylinder, a piston, a piston rod, a liquid suction pipe, a liquid outlet pipe and a power mechanism. The dyeing unit performs continuous dyeing operation, the automation degree is high, and the working efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell staining devices, in particular to a biological science cell staining device. Background Art

[0002] In experiments, in order to clearly observe the target tissue or cells, staining is usually used for visual verification.

[0003] After searching, Chinese patent CN214373751U discloses a cell staining device, comprising a base, on which a crank slider mechanism is rotatably mounted, the crank slider mechanism is connected to a slide stage, the slide stage is slidably connected to the base, a bracket is provided above the slide stage, the bracket is provided with an electric push rod, the electric push rods are connected to a plurality of dripping bottles in sequence, a baffle connected to the bracket is provided below the dripping bottle, a through hole intermittently connected to the dripping bottle is provided at the center of the baffle, a hair dryer is provided on the left side of the bracket, and a flushing mechanism is provided on the right side of the bracket.

[0004] Although the above-mentioned prior art can perform cell staining, it can only stain cells on one slide at a time. Only after the staining is completed can the slide be removed and replaced with another one. This operation is more troublesome and reduces the efficiency of the staining work. Summary of the Invention

[0005] The utility model provides a biological science cell staining device, aiming to solve the above problems.

[0006] The utility model is implemented as follows: a biological science cell staining device, comprising:

[0007] A plurality of placement seats are arranged at intervals in a circular direction, each of which is provided with a placement groove for placing a glass slide;

[0008] A driving mechanism for driving the multiple placement seats to rotate intermittently;

[0009] A dyeing unit is provided above the rotation track of the placement seat, and a dyeing station is provided below the liquid outlet of the dyeing unit;

[0010] The positioning structure installed on the driving mechanism is used to synchronously fix the glass slides on all the placement seats.

[0011] Preferably, the driving mechanism comprises:

[0012] base plate;

[0013] A first stepper motor fixed to the bottom plate at the center of the plurality of placement seats;

[0014] A mounting plate fixed to the output shaft of the first stepper motor;

[0015] A plurality of sleeves are circumferentially installed on the side wall of the mounting plate at intervals, and one end of each sleeve away from the mounting plate is fixedly connected to a placement seat.

[0016] Preferably, the positioning structure includes:

[0017] A movable cavity is formed in the sleeve along its length, a driving rod is movably provided in the movable cavity, one end of the driving rod extends into the placement groove and is fixed with a positioning plate;

[0018] An air pump fixed to the top of the mounting plate, the air pump being connected to a main air pipe, the main air pipe being connected to a plurality of bronchial tubes, the bronchial tubes being connected to the sleeves in a one-to-one correspondence, and the bronchial tubes being connected to an end of the movable cavity away from the placement seat;

[0019] An elastic rope is connected between the positioning plate and the placement seat, and the elastic rope exerts a force on the positioning plate toward one side of the mounting plate.

[0020] Preferably, the positioning structure further includes:

[0021] A plate body is arranged opposite to the positioning plate, the plate body is slidably connected to the placement groove, a pin is fixed on the side wall of the plate body away from the positioning plate, one end of the pin passes through the end wall of the placement seat and is provided with a pin head;

[0022] Side rods are provided on both sides of the placement seat, one end of the side rod is hinged to the end of the placement seat away from the positioning plate, the other end of the side rod is fixed with a rubber protrusion, and the side wall of the placement seat is provided with a through groove for the side rod and the rubber protrusion to move;

[0023] An elastic rod is fixed to the end of the side rod away from the rubber protrusion, and the elastic rod extends to the side wall of the plate body away from the positioning plate.

[0024] Preferably, the dyeing unit comprises:

[0025] An injection cylinder is vertically fixed on a bracket, the bracket is fixed on a bottom plate, the bottom end of the injection cylinder is connected to a liquid outlet pipe, a piston is provided in the injection cylinder, a piston rod is fixed on the piston, a side wall of the injection cylinder is connected to a liquid pipe, one end of the liquid pipe is connected to the bottom of the injection cylinder cavity, and the other end of the liquid pipe is connected to the bottom of the liquid tank cavity, the liquid tank is fixed on the bracket or the bottom plate, and contains a dye, and a one-way valve is installed on the liquid pipe and the liquid outlet pipe;

[0026] A power mechanism used to drive the piston rod up and down.

[0027] Preferably, the power mechanism comprises:

[0028] a second stepper motor fixed on the bracket, wherein a turntable is fixed on an output shaft of the second stepper motor;

[0029] A movable rod is horizontally arranged on the front side of the turntable, a pin is eccentrically installed on the turntable, a movable groove for the pin to be inserted and moved is opened on the movable rod, vertical guide rods are passed through both ends of the movable rod, the guide rod is fixedly connected to the bracket, and the piston rod is fixedly connected to the movable rod.

[0030] Preferably, a threaded rod is radially rotatably mounted on the end surface of the turntable, an internal threaded sleeve is threadedly connected to the threaded rod, the internal threaded sleeve is slidably connected to the end surface of the turntable, and the pin is fixed on the internal threaded sleeve.

[0031] Preferably, one end of the sleeve is hinged to the mounting groove on the side wall of the mounting plate, the bottom of the mounting groove is located at the lower side of the sleeve and is provided with a groove, a tension spring is installed in the groove, and the two ends of the tension spring are respectively fixed to the bottom of the groove and the bottom of the sleeve, an arc-shaped plate that cooperates with the placement seat is fixed to the bottom plate, a plurality of first protrusions are fixed at intervals on the upper surface of the arc-shaped plate, and a second protrusion that cooperates with the first protrusion is fixed on the lower surface of the placement seat. After dyeing is completed, the driving mechanism drives the placement seat to rotate, and the second protrusion on the placement seat contacts and cooperates with the plurality of first protrusions to cause the placement seat to vibrate.

[0032] Compared with the prior art, the embodiments of the present application have the following beneficial effects:

[0033] The biological science cell staining device provided by the utility model is provided with multiple placement seats, a driving mechanism, a staining unit and a positioning structure. The driving machine drives the multiple placement seats to move to the staining station one by one, and the staining unit performs continuous staining operations, which has a high degree of automation and improves work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a schematic structural diagram of a biological science cell staining device provided by the utility model;

[0035] Figure 2 This is a top view and partial cross-sectional view of a mounting plate and a mounting seat in a biological science cell staining device provided by the utility model;

[0036] Figure 3 This is a structural diagram of a turntable, a threaded rod and an internal threaded sleeve in a biological science cell staining device provided by the utility model;

[0037] Figure 4 The utility model is a top view of a curved plate in a biological science cell staining device.

[0038] Notes on the accompanying drawings: 1. Base plate; 2. First stepper motor; 3. Mounting plate; 4. Sleeve; 5. Placement seat; 6. Side rod; 7. Mounting groove; 8. Tension spring; 9. Air pump; 10. Liquid tank; 11. Suction tube; 12. Liquid outlet pipe; 13. Injection cylinder; 14. Piston rod; 15. Movable rod; 16. Turntable; 17. Guide rod; 18. Arc plate; 19. Internal threaded sleeve; 191. Threaded rod; 20. Elastic rod; 21. Plate body; 22. Positioning plate; 23. Rubber protrusion; 24. Elastic rope. DETAILED DESCRIPTION

[0039] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art to which this application belongs. The terms used in the specification of the application are only for the purpose of describing specific embodiments and are not intended to limit this application. The terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification and claims of this application or the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.

[0040] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0041] The present invention provides a biological science cell staining device. Figures 1-4 Shown, including:

[0042] A plurality of placement seats 5 are arranged at intervals in a circular direction, each of which has a placement groove for placing a slide;

[0043] A driving mechanism for driving the multiple placement seats 5 to rotate intermittently;

[0044] A dyeing unit is provided above the rotation track of the placement seat 5, and a dyeing station is provided below the liquid outlet of the dyeing unit;

[0045] The positioning structure installed on the driving mechanism is used to synchronously fix the glass slides on all the placement seats 5;

[0046] During operation, the driving machine drives the plurality of placement seats 5 to move to the dyeing stations one by one, and the dyeing units perform continuous dyeing operations, which has a high degree of automation and improves work efficiency.

[0047] Wherein, the driving mechanism includes:

[0048] Base plate 1;

[0049] The first stepper motor 2 is fixed on the bottom plate 1 at the center of the plurality of placement seats 5 and can be fixed by bolts;

[0050] The mounting plate 3 is fixed on the output shaft of the first stepper motor 2 and can be fixed by screws;

[0051] A plurality of sleeves 4 are circumferentially spaced apart and mounted on the side wall of the mounting plate 3, wherein one end of each sleeve 4 away from the mounting plate 3 is fixedly connected to a placement seat 5;

[0052] The first stepper motor 2 drives the mounting plate 3 to rotate intermittently, and the mounting plate 3 drives the placement seat 5 to rotate intermittently through the sleeve 4.

[0053] In this embodiment, the positioning structure includes:

[0054] The sleeve 4 is provided with a movable cavity along its length, and a driving rod is movably provided in the movable cavity. One end of the driving rod extends into the placement groove and is fixed with a positioning plate 22.

[0055] An air pump 9 is fixed to the top of the mounting plate 3. The air pump 9 is connected to a main air pipe, and the main air pipe is connected to a plurality of bronchi. The bronchi are connected to the sleeves 4 in a one-to-one correspondence and can be connected via a pipe joint. The bronchi are connected to the end of the active cavity away from the placement seat 5.

[0056] An elastic rope 24 is connected between the positioning plate 22 and the placement seat 5 , and the elastic rope 24 applies a force to the positioning plate 22 toward the mounting plate 3 .

[0057] When the glass slide needs to be fixed, the air pump 9 is inflated, and the air enters the bronchus through the main trachea, and then enters the active cavity, pushing the driving rod to drive the positioning plate 22 close to the glass slide, and cooperating with the groove wall of the placement groove to fix the glass slide. When the glass slide needs to be loosened, the air pump 9 is deflated, the air pressure in the active cavity is reduced, and the elastic rope 24 pulls the positioning plate 22 to reset.

[0058] Furthermore, the positioning structure further includes:

[0059] The plate body 21 is arranged opposite to the positioning plate 22, and the plate body 21 is slidably connected to the placement groove, which can be slidably connected by a slider and a slide groove. A pin is fixed on the side wall of the plate body 21 away from the positioning plate 22, and one end of the pin passes through the end wall of the placement seat 5 and is provided with a nail head;

[0060] Side rods 6 are provided on both sides of the placement seat 5, one end of the side rod 6 is hinged to the end of the placement seat 5 away from the positioning plate 22, and the other end of the side rod 6 is fixed with a rubber protrusion 23. The side wall of the placement seat 5 is provided with a through groove for the side rod 6 and the rubber protrusion 23 to move;

[0061] The elastic rod 20 is fixed to one end of the side rod 6 away from the rubber protrusion 23 , and the elastic rod 20 extends to the side wall of the plate body 21 away from the positioning plate 22 .

[0062] When the driving rod drives the positioning plate 22 to move toward the side of the plate body 21, it cooperates with the plate body 21 to clamp the two ends of the glass slide. At the same time, it pushes the plate body 21 to move away from the driving rod. The plate body 21 pushes the elastic rod 20, and the elastic rod 20 drives the side rod 6 to rotate into the placement groove. The rubber protrusion 23 presses against the side of the glass slide, thereby stably fixing the glass slide.

[0063] In a specific implementation, the dyeing unit includes:

[0064] An injection cylinder 13 is vertically fixed to a bracket, the bracket being fixed to the base plate 1. The bottom end of the injection cylinder 13 is connected to a liquid outlet pipe 12. A piston is provided in the injection cylinder 13, and a piston rod 14 is fixed to the piston. A liquid pipe 11 is connected to the side wall of the injection cylinder 13. One end of the liquid pipe 11 is connected to the bottom of the inner cavity of the injection cylinder 13, and the other end of the liquid pipe 11 is connected to the bottom of the inner cavity of the liquid tank 10. The liquid tank 10 is fixed to the bracket or the base plate 1 and contains a dye. Both the liquid pipe 11 and the liquid outlet pipe 12 are installed with a one-way valve.

[0065] A power mechanism for driving the piston rod 14 up and down;

[0066] During operation, the power mechanism drives the piston rod 14 to move upward, sucking the dye in the liquid tank 10 into the inner cavity of the injection cylinder 13, and then the power mechanism drives the piston rod 14 to move downward, pushing the dye in the injection cylinder 13 down and discharging it from the liquid outlet pipe 12, dripping onto the glass slide for staining.

[0067] Furthermore, the power mechanism includes:

[0068] The second stepper motor fixed on the bracket can be fixed by bolts, and the output shaft of the second stepper motor is fixed with a turntable 16, which can be fixed by screws;

[0069] A movable rod 15 is horizontally arranged in front of the turntable 16, and a pin is eccentrically mounted on the turntable 16. The movable rod 15 is provided with a movable slot for the pin to be inserted and moved. Vertical guide rods 17 are passed through both ends of the movable rod 15. The guide rods 17 are fixedly connected to the bracket, and the piston rod 14 is fixedly connected to the movable rod 15;

[0070] During operation, the second stepper motor is started, the second stepper motor drives the turntable 16 to rotate, the turntable 16 drives the pin to revolve, the pin drives the movable rod 15 to move up and down along the guide rod 17, and the guide rod 17 drives the piston rod 14 to move up and down.

[0071] Furthermore, a threaded rod 191 is radially rotatably mounted on the end surface of the turntable 16 and can be rotatably mounted via a bearing. An internally threaded sleeve 19 is threadedly connected to the threaded rod 191. The internally threaded sleeve 19 is slidably connected to the end surface of the turntable 16, and the pin is fixed to the internally threaded sleeve 19.

[0072] It can be understood that by rotating the threaded rod 191, the threaded rod 191 drives the internal threaded sleeve 19 to move, adjusting the position of the internal threaded sleeve 19 on the end face of the turntable 16, thereby adjusting the distance between the pin shaft and the center of the turntable 16, adjusting the rotation diameter of the pin shaft, and thereby adjusting the reciprocating motion distance of the movable rod 15, the piston rod 14 and the piston, thereby achieving the effect of adjusting the amount of dye discharged and meeting more work requirements.

[0073] In this embodiment, one end of the sleeve 4 is hinged to the mounting groove 7 on the side wall of the mounting plate 3, and the bottom of the mounting groove 7 is located on the lower side of the sleeve 4 and is provided with a groove. A tension spring 8 is installed in the groove, and the two ends of the tension spring 8 are respectively fixed to the bottom of the groove and the bottom of the sleeve 4. An arc-shaped plate 18 that cooperates with the placement seat 5 is fixed on the bottom plate 1, and a plurality of first protrusions are fixed at intervals on the upper surface of the arc-shaped plate 18. A second protrusion that cooperates with the first protrusion is fixed on the lower surface of the placement seat 5. After the staining is completed, the driving mechanism drives the placement seat 5 to rotate, and the second protrusion on the placement seat 5 contacts and cooperates with the plurality of first protrusions, causing the placement seat 5 to vibrate, thereby dispersing the dye on the glass slide, which is beneficial to improving the staining effect.

[0074] In summary, the present invention provides a biological science cell staining device, the working principle of which is as follows:

[0075] A glass slide is placed in each placement seat 5, and the air pump 9 is inflated. The air enters the bronchus through the main trachea, and then enters the movable cavity, pushing the driving rod to drive the positioning plate 22 close to the glass slide until the glass slide is fixed, and then the first stepper motor 2 drives the mounting disk 3 to rotate intermittently, and the mounting disk 3 drives the placement seat 5 to rotate intermittently through the sleeve 4, driving multiple placement seats 5 to move to the dyeing station one by one, and the dyeing unit performs continuous dyeing operations. The second stepper motor is started, and the second stepper motor drives the turntable 16 to rotate. The turntable 16 drives the pin to revolve, and the pin drives the piston rod 14 to move upward through the movable rod 15, and the dye in the liquid tank 10 is sucked into the inner cavity of the injection cylinder 13. Then the power mechanism drives the piston rod 14 to move downward, and pushes the dye in the injection cylinder 13 down to be discharged from the liquid outlet pipe 12, dripping onto the glass slide for dyeing. The degree of automation is high, and the work efficiency is improved.

[0076] It should be noted that, for the sake of simplicity, the aforementioned embodiments are described as a series of action combinations. However, those skilled in the art should be aware that the present invention is not limited by the order of the actions described, because according to the present invention, certain steps may be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in this specification are all preferred embodiments, and the actions and modules involved are not necessarily required by the present invention.

[0077] The above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on these embodiments, all other embodiments derived by persons of ordinary skill in the art without inventive effort are also within the scope of protection of the present invention.

Claims

1. A biological science cell staining device, characterized in that: include: A plurality of placement seats are arranged at intervals in a circular direction, each of which is provided with a placement groove for placing a glass slide; A driving mechanism for driving the multiple placement seats to rotate intermittently; A dyeing unit is provided above the rotation track of the placement seat, and a dyeing station is provided below the liquid outlet of the dyeing unit; The positioning structure installed on the driving mechanism is used to synchronously fix the glass slides on all the placement seats.

2. The biological science cell staining device according to claim 1, wherein: The driving mechanism comprises: base plate; A first stepper motor fixed to the bottom plate at the center of the plurality of placement seats; A mounting plate fixed to the output shaft of the first stepper motor; A plurality of sleeves are circumferentially installed on the side wall of the mounting plate at intervals, and one end of each sleeve away from the mounting plate is fixedly connected to a placement seat.

3. The biological science cell staining device according to claim 2, wherein: The positioning structure includes: A movable cavity is formed in the sleeve along its length, a driving rod is movably provided in the movable cavity, one end of the driving rod extends into the placement groove and is fixed with a positioning plate; An air pump fixed to the top of the mounting plate, the air pump being connected to a main air pipe, the main air pipe being connected to a plurality of bronchial tubes, the bronchial tubes being connected to the sleeves in a one-to-one correspondence, and the bronchial tubes being connected to an end of the movable cavity away from the placement seat; An elastic rope is connected between the positioning plate and the placement seat, and the elastic rope exerts a force on the positioning plate toward one side of the mounting plate.

4. The biological science cell staining device according to claim 3, wherein: The positioning structure further includes: A plate body is arranged opposite to the positioning plate, the plate body is slidably connected to the placement groove, a pin is fixed on the side wall of the plate body away from the positioning plate, one end of the pin passes through the end wall of the placement seat and is provided with a pin head; Side rods are provided on both sides of the placement seat, one end of the side rod is hinged to the end of the placement seat away from the positioning plate, the other end of the side rod is fixed with a rubber protrusion, and the side wall of the placement seat is provided with a through groove for the side rod and the rubber protrusion to move; An elastic rod is fixed to the end of the side rod away from the rubber protrusion, and the elastic rod extends to the side wall of the plate body away from the positioning plate.

5. The biological science cell staining device according to claim 1, wherein: The dyeing unit comprises: An injection cylinder is vertically fixed on a bracket, the bracket is fixed on a bottom plate, the bottom end of the injection cylinder is connected to a liquid outlet pipe, a piston is provided in the injection cylinder, a piston rod is fixed on the piston, a side wall of the injection cylinder is connected to a liquid pipe, one end of the liquid pipe is connected to the bottom of the injection cylinder cavity, and the other end of the liquid pipe is connected to the bottom of the liquid tank cavity, the liquid tank is fixed on the bracket or the bottom plate, and contains a dye, and a one-way valve is installed on the liquid pipe and the liquid outlet pipe; A power mechanism used to drive the piston rod up and down.

6. The biological science cell staining device according to claim 5, characterized in that: The power mechanism comprises: a second stepper motor fixed on the bracket, wherein a turntable is fixed on an output shaft of the second stepper motor; A movable rod is horizontally arranged on the front side of the turntable, a pin is eccentrically installed on the turntable, a movable groove for the pin to be inserted and moved is opened on the movable rod, vertical guide rods are passed through both ends of the movable rod, the guide rod is fixedly connected to the bracket, and the piston rod is fixedly connected to the movable rod.

7. The biological science cell staining device according to claim 6, characterized in that: A threaded rod is mounted on the end surface of the turntable in a radially rotatable manner. An internal threaded sleeve is threadedly connected to the threaded rod. The internal threaded sleeve is slidably connected to the end surface of the turntable, and the pin is fixed on the internal threaded sleeve.

8. The biological science cell staining device according to claim 2, wherein: One end of the sleeve is hinged to the mounting groove on the side wall of the mounting plate, and the bottom of the mounting groove is located at the lower side of the sleeve and is provided with a groove, and a tension spring is installed in the groove, and the two ends of the tension spring are respectively fixed to the bottom of the groove and the bottom of the sleeve, and an arc-shaped plate that cooperates with the placement seat is fixed on the bottom plate, and a plurality of first protrusions are fixed at intervals on the upper surface of the arc-shaped plate, and a second protrusion that cooperates with the first protrusion is fixed on the lower surface of the placement seat. After dyeing is completed, the driving mechanism drives the placement seat to rotate, and the second protrusion on the placement seat contacts and cooperates with the plurality of first protrusions to cause the placement seat to vibrate.

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