Reagent cup for semi-quantitative detection of human chorionic gonadotropin

Abstract

The utility model relates to the technical field of biological detection, in particular to a reagent cup for semi-quantitative detection of human chorionic gonadotropin. A reagent cup for semi-quantitative detection of human chorionic gonadotropin comprises a cup body, the cup body is provided with a first side wall and a second side wall, an insertion groove is formed in the outer side face of the first side wall, a test strip is suitable for being placed in the insertion groove, a hollow groove is formed in the second side wall and communicated with the bottom of the cup body, and the hollow groove is communicated with the bottom of the cup body. The hollow groove is suitable for accommodating a test strip; the cup cover is matched with the cup body, the cup cover is provided with a containing groove, tweezers are arranged in the containing groove, and the tweezers are used for moving the test strip in the inserting groove into the hollow groove. The reagent cup for semi-quantitative detection solves the problems that a plurality of test strips are fixed in the cup body, and when the reagent cup for semi-quantitative detection is used by pregnant women in different pregnancy periods, even if the plurality of test strips are not needed, the test strips are still soaked by test liquid to be detected, so that the test strips are wasted.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a reagent cup for semi-quantitative detection of human chorionic gonadotropin. Background Art

[0002] Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by placental trophoblast cells during pregnancy. It consists of two distinct subunits, α and β, linked by a non-covalent bond. During hormone production, secretion, and metabolism, hCG molecules undergo various changes, including fragmentation and dissociation, resulting in their presence in various molecular forms in blood and urine.

[0003] Currently, human chorionic gonadotropin (hCG) testing is widely used in the diagnosis and treatment of conditions such as threatened abortion, inevitable abortion, missed abortion, ectopic pregnancy, and trophoblastic lesions. By measuring hCG concentration, it can be used to diagnose and differentiate various diseases and observe their course, which is of great clinical significance. According to the "Chinese Clinical Laboratory Operation Procedures," hCG levels vary among pregnant women at different stages of pregnancy. Existing semi-quantitative test cups often hold multiple test strips within the cup. This results in test strips being soaked with the test fluid during testing, even when pregnant women at different stages of pregnancy do not need to use multiple test strips, resulting in waste of test strips. Utility Model Content

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the semi-quantitative detection reagent cup in the prior art often fixes multiple test strips in the cup body. This will result in pregnant women in different gestational stages using the cup, even if they do not need to use multiple test strips, the test strips will still be soaked in the test liquid to be tested, resulting in waste of the test strips, thereby providing a reagent cup for semi-quantitative detection of human chorionic gonadotropin.

[0005] In order to solve the above problems, the utility model provides a reagent cup for semi-quantitative detection of human chorionic gonadotropin, comprising:

[0006] A cup body, the cup body having a first side wall and a second side wall, the first side wall having a slot on its outer side, the slot being suitable for placing a test strip, the second side wall having a hollow groove, the hollow groove being connected to the bottom of the cup body, the hollow groove being suitable for accommodating a test strip;

[0007] The cup cover is adapted to the cup body, and the cup cover is provided with a receiving slot, in which tweezers are provided for moving the test strip in the slot into the hollow slot.

[0008] Optionally, the number of the slots and hollow slots is at least two respectively.

[0009] Optionally, adjacent hollow grooves are spaced apart, and the bottom surface of the hollow groove is higher than the inner bottom surface of the cup body.

[0010] Optionally, the test strip includes a substrate, and a sample loading pad, a colloidal gold adsorption pad, an antibody carrying film and a water absorbent pad adhered to the substrate in sequence, and the sample loading pad, colloidal gold adsorption pad, antibody carrying film and water absorbent pad are overlapped in sequence; on the antibody carrying film, a detection line is provided at one end close to the colloidal gold adsorption pad, and a quality control line is provided at one end close to the water absorbent pad.

[0011] Optionally, the absorbent pad of the test strip is provided with a circular hole, and the circular hole is adapted to the picking end of the tweezers.

[0012] Optionally, the absorbent pad of the test strip extends from the opening of the slot to the outside of the cup body.

[0013] Optionally, the cup body is made of one of PVC, TPE, and PE.

[0014] Optionally, the cup body is transparent and is provided with scale lines.

[0015] Optionally, the length of the test strip is greater than or equal to the length of the cup body.

[0016] The technical solution of this utility model has the following advantages:

[0017] 1. The reagent cup for semi-quantitative detection of human chorionic gonadotropin provided by the present invention includes: a cup body, having a first side wall and a second side wall, a slot is provided on the outer surface of the first side wall, the slot is suitable for placing a test strip, and the second side wall is provided with a hollow groove, which is connected to the bottom of the cup body and is suitable for accommodating the test strip; a cup cover, which is adapted to the cup body, and the cup cover is provided with a accommodating groove, and tweezers are provided in the accommodating groove, and the tweezers are used to move the test strip in the slot into the hollow groove.

[0018] Before use, the test strips are placed in the slots. When they are needed, tweezers are used to move the test strips from the slots to the hollow slots. Users can select the appropriate number of test strips to place in the hollow slots based on test requirements, without having to place all of them. This saves test strips and eliminates the need to use all of them at once. Tweezers are also provided to prevent contamination of the test strips and ensure their cleanliness.

[0019] 2. The reagent cup for semi-quantitative detection of human chorionic gonadotropin provided by the present invention has at least two slots and at least two hollow slots. By providing at least two slots and at least two hollow slots, sufficient test strips are provided to the user, and it is also convenient for the user to select the corresponding number of test strips.

[0020] 3. The present invention provides a semi-quantitative human chorionic gonadotropin test cup with adjacent hollow slots spaced apart, with the bottom of each slot higher than the inner bottom of the cup. The cup collects the test solution, and a test strip inserted into the hollow slot is then inserted into the test solution for testing.

[0021] 4. The present invention provides a reagent cup for semi-quantitative detection of human chorionic gonadotropin. The test strip comprises a substrate, and a sample loading pad, a colloidal gold adsorption pad, an antibody-carrying membrane, and a water-absorbing pad adhered sequentially to the substrate. The sample loading pad, colloidal gold adsorption pad, antibody-carrying membrane, and water-absorbing pad are sequentially joined by partial overlap. The antibody-carrying membrane is provided with a test line at one end near the colloidal gold adsorption pad, and a quality control line at one end near the water-absorbing pad. This structure allows the test fluid to pass through the sample loading pad, then reach the test line, the quality control line, and finally the water-absorbing pad, facilitating on-site observation by the user.

[0022] 5. The reagent cup for semi-quantitative detection of human chorionic gonadotropin provided by the utility model has a circular hole on the absorbent pad of the test strip, which is adapted to the picking end of the tweezers. The setting of the circular hole facilitates the tweezers to apply force through the picking end to grasp.

[0023] 6. In the reagent cup for semi-quantitative detection of human chorionic gonadotropin provided by the present invention, the absorbent pad of the test strip extends from the opening of the slot to the outside of the cup body, so that it can be easily grasped by tweezers and placed into the hollow slot.

[0024] 7. The reagent cup for semi-quantitative detection of human chorionic gonadotropin provided by the present invention has a cup body made of one of PVC, TPE, and PE. The above three materials are low in cost and suitable for use in medical devices.

[0025] 8. The reagent cup for semi-quantitative detection of human chorionic gonadotropin provided by the present invention has a transparent cup body with scale lines. The transparent setting makes it easy for users to observe the changes in the test strips, and the scale lines specify the minimum depth in the cup body.

[0026] 9. The reagent cup for semi-quantitative detection of human chorionic gonadotropin provided by the present invention has a test strip whose length is greater than or equal to the length of the cup body, so that the sample pad of the test strip can enter the liquid in the cup body through the hollow groove. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0028] Figure 1 A schematic diagram of a cup body provided in an embodiment of the present utility model;

[0029] Figure 2 for Figure 1 A cross-sectional view of AA is shown;

[0030] Figure 3 A schematic diagram of a reagent paper provided in an embodiment of the present invention;

[0031] Figure 4 It is a schematic structural diagram of the reagent paper provided in the embodiment of the utility model.

[0032] Explanation of the accompanying symbols: 1. cup body; 11. scale line; 12. first side wall; 13. second side wall; 2. slot; 3. hollow groove; 4. test strip; 41. sample loading pad; 42. colloidal gold adsorption pad; 43. antibody carrying membrane; 44. water-absorbing pad; 45. circular hole; 46. substrate; 47. detection line; 48. quality control line; 49. protective film. DETAILED DESCRIPTION

[0033] The following is a clear and complete description of the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0034] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating positions or relationships, are based on the positions or relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0035] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0036] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0037] like Figure 1-4 A specific embodiment of a reagent cup for semi-quantitative detection of human chorionic gonadotropin is shown, comprising: a cup body 1 and a cup cover adapted to the cup body 1, wherein the cup body 1 is suitable for collecting a test fluid to be tested, specifically, the test fluid to be tested is urine of a pregnant woman.

[0038] like Figure 1 、 Figure 2 As shown, the cup body 1 has a first side wall 12 and a second side wall 13, wherein the outer surface of the first side wall 12 is provided with five slots 2, each slot 2 is suitable for placing a test strip 4, and the second side wall 13 is provided with five hollow grooves 3, the hollow grooves 3 are connected to the bottom of the cup body 1, and the hollow grooves 3 are suitable for accommodating the test strips 4. It should be noted that the hollow grooves 3 and the slots 2 are provided in a one-to-one correspondence. Figure 1 、 Figure 2 As shown, adjacent hollow grooves 3 are spaced apart, and the bottom surface of the hollow groove 3 is higher than the inner bottom surface of the cup body 1. It should be noted that the bottom surface of the hollow groove 3 in this application refers to the bottom surface of the inner side wall of the second side wall 13 where the hollow groove 3 is opened, so that the test strip 4 entering the hollow groove 3 can reach the test liquid to be tested in the cup body 1. For easy observation, the cup body 1 is transparent and is made of PVC (polyvinyl chloride). Figure 2 As shown, the cup body 1 is provided with scale lines 11, which confirm the lowest value of the test liquid to be tested in the cup body 1. To ensure that the test strip 4 can enter the bottom of the cup body 1, the length of the test strip 4 is greater than the length of the cup body 1.

[0039] A receiving groove is provided on the upper surface of the cup cover, and tweezers are provided in the receiving groove for moving the test strip 4 in the slot 2 into the hollow groove 3 .

[0040] like Figure 3 、 Figure 4 As shown, the test strip 4 includes a substrate 46 and a sample loading pad 41, a colloidal gold adsorption pad 42, an antibody carrying film 43 and a water absorbent pad 44 adhered to the substrate 46 in sequence. The sample loading pad 41, the colloidal gold adsorption pad 42, the antibody carrying film 43 and the water absorbent pad 44 are overlapped in sequence. Specifically, the antibody carrying film 43 is located below the colloidal gold adsorption pad 42 and the water absorbent pad 44, and the sample loading pad 41 is located above the colloidal gold adsorption pad 42. Figure 4As shown, on the antibody carrying film 43, a detection line 47 is provided at one end close to the colloidal gold adsorption pad 42, and a quality control line 48 is provided at one end close to the water absorbent pad 44; the detection line 47 is coated with anti-human chorionic gonadotropin α-hCG monoclonal antibody (anti-α-hCG monoclonal antibody), and the quality control line 48 is coated with anti-mouse IgG polyclonal antibody. Figure 4 As shown, the absorbent pad 44 of the test strip 4 is provided with a circular hole 45 , which is adapted to fit the picking end of the tweezers, and the test strip 4 is covered with a protective film 49 .

[0041] To facilitate testing, the colloidal gold adsorption pads 42 of different test strips 4 contain different adsorbents. For example, the colloidal gold adsorption pad 42 of the first test strip 4 contains a colloidal gold-anti-human chorionic gonadotropin β-hCG monoclonal antibody conjugate (colloidal gold-anti-β-hCG monoclonal antibody conjugate). The colloidal gold adsorption pads 42 of the second, third, fourth, and fifth test strips 4 contain a colloidal gold-anti-β-hCG monoclonal antibody conjugate and free β-antibody. The mass ratios of the adsorbed colloidal gold-anti-β-hCG monoclonal antibody conjugate to the free β-antibody are 1:2, 1:40, 1:80, and 1:100, respectively. The free β-antibody is a non-mouse antibody. Markings are provided on the test strips 4 to facilitate user identification and selection of different test strips 4.

[0042] During the specific implementation process, the user can select the corresponding number of test strips 4 according to their pregnancy stage and actual situation. First, use tweezers to pick up the corresponding number of test strips 4 and place them into the hollow slot 3. After pulling the test strips 4 out of the slot 2, it is necessary to tear off the protective film 49, collect the test liquid to be tested, and slowly combine the test liquid with the test strips 4. Taking the example of placing all five test strips 4 in the cup body 1 of the present application, the sample pads 41 of the five test strips 4 are immersed in the sample. Due to capillary action, the sample is sucked into the test strips 4 by the sample pad 41, passes through the colloidal gold adsorption pad 42, the antibody carrier membrane 43, and reaches the water absorbent pad 44. The hCG in the sample combines with the colloidal gold-anti-β-hCG monoclonal antibody conjugate or free β antibody adsorbed on the colloidal gold adsorption pad 42. hCG bound to the colloidal gold-anti-β-hCG monoclonal antibody conjugate reaches the antibody-carrying membrane 43 as the liquid flows, and binds to the anti-α-hCG monoclonal antibody coated in the test line 47 and the anti-mouse IgG polyclonal antibody coated in the quality control line 48, respectively. Since hCG is labeled with colloidal gold, it will show color at the test line 47 and the quality control line 48. Since hCG bound to free β-antibodies is not labeled with colloidal gold, it will not develop color at test line 47 . Furthermore, since the free β-antibodies are not mouse-derived antibodies, they will not bind to the anti-mouse IgG polyclonal antibodies coated on quality control line 48 . Since the colloidal gold adsorption pads 42 of the five test strips 4 contain different ratios of colloidal gold-anti-β-hCG monoclonal antibody conjugate and free β-antibodies, the test lines 47 of the five test strips 4 will exhibit different color developments. Based on the color development of each test strip 4 , semi-quantitative detection of hCG concentration in urine can be performed. Compared to using two test strips, the detectable concentration range is wider, and there is no need to compare and judge based on the degree of color development, resulting in more accurate test results.

[0043] The utility model provided by the present invention has the following advantages: (1) users can select the corresponding number of test strips 4 according to their own needs, without having to select all the test strips 4, which is convenient for saving test strips 4 and does not require all the test strips 4 to be used at one time; (2) manufacturers can make cup bodies 1 containing different numbers of test strips 4 according to pregnant women in different gestational stages for users to use; (3) the combination of different test strips 4 can obtain a more accurate detection structure and improve the accuracy of detection; (4) tweezers are provided to prevent the test strips 4 from being contaminated and ensure that the test strips 4 are clean.

[0044] As an alternative embodiment, the number of the slots 2 and the hollow slots 3 may be 2, 3, 4, 6 or even more.

[0045] As an alternative embodiment, the numbers of the slots 2 and the hollow grooves 3 in the same cup body 1 may be unequal.

[0046] As an alternative embodiment, the cup body 1 may also be made of other materials such as PE (ultra-low density polyethylene), TPE (polyolefin elastomer), etc.

[0047] As an alternative embodiment, the length of the test strip 4 may also be equal to the length of the cup body 1 .

[0048] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications derived therefrom remain within the scope of protection of the present invention.

Claims

1. A reagent cup for semi-quantitative detection of human chorionic gonadotropin, characterized in that: include: A cup body (1), the cup body (1) having a first side wall (12) and a second side wall (13), the first side wall (12) having a slot (2) on its outer surface, the slot (2) being suitable for placing a test strip (4), the second side wall (13) having a hollow groove (3), the hollow groove (3) being in communication with the bottom of the cup body (1), the hollow groove (3) being suitable for accommodating the test strip (4); A cup cover is adapted to the cup body (1), the cup cover is provided with a receiving groove, and tweezers are provided in the receiving groove, and the tweezers are used to move the test strip (4) in the slot (2) into the hollow groove (3).

2. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 1, characterized in that: The number of the slots (2) and the number of the hollow slots (3) are at least two respectively.

3. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 2, characterized in that: Adjacent hollow grooves (3) are spaced apart, and the bottom surface of the hollow groove (3) is higher than the inner bottom surface of the cup body (1).

4. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 1, characterized in that: The test strip (4) includes a substrate (46), and a sample loading pad (41), a colloidal gold adsorption pad (42), an antibody carrying film (43) and a water absorbent pad (44) adhered to the substrate (46) in sequence, wherein the sample loading pad (41), the colloidal gold adsorption pad (42), the antibody carrying film (43) and the water absorbent pad (44) are overlapped in sequence; a detection line (47) is provided on the antibody carrying film (43) at one end close to the colloidal gold adsorption pad (42), and a quality control line (48) is provided at one end close to the water absorbent pad (44).

5. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 4, characterized in that: The absorbent pad (44) of the test strip (4) is provided with a circular hole (45), and the circular hole (45) is adapted to fit the picking end of the tweezers.

6. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 5, characterized in that: The absorbent pad (44) of the test strip (4) extends from the opening of the slot (2) to the outside of the cup body (1).

7. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 6, characterized in that: The material of the cup body (1) is one of PVC, TPE, and PE.

8. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 7, characterized in that: The cup body (1) is transparent and is provided with scale lines (11).

9. The reagent cup for semi-quantitative detection of human chorionic gonadotropin according to claim 8, characterized in that: The length of the test strip (4) is greater than or equal to the length of the cup body (1).

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