Pipetting device

Abstract

The utility model relates to the technical field of pipetting, in particular to a pipetting device which comprises a pumping cylinder, a suction head is connected to the bottom of the pumping cylinder, a piston is connected to the inner wall of the suction head in a sliding mode, and an adjusting assembly, an injection assembly and a suction assembly are arranged on the outer wall of the pumping cylinder. According to the invention, the magnetic slide block is driven to slide in the slide way by pushing the push block, and the quantity of the culture solution required at a single time is determined according to the position of the scale line corresponding to the bottom of the push block; when the magnetic sliding block slides, the magnetic check block is pulled to move through the magnetism of the magnetic sliding block so as to limit the piston, when the culture solution is sucked, the piston slides on the inner wall of the suction cylinder, and when the piston slides to be in contact with the magnetic check block, suction is stopped at the moment.

Description

Technical Field

[0001] The utility model relates to the technical field of liquid transfer, in particular to a liquid transfer device. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH, and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology, and its formal term in biology is cell culture technology. Cell culture is an indispensable process for both the entire bioengineering technology and one of its components, biological cloning technology. Cell culture itself is the large-scale cloning of cells. Cell culture technology can transform a single cell into a simple single cell or minimally differentiated multi-cell through large-scale culture. This is an indispensable part of cloning technology, and cell culture itself is the cloning of cells. Cell culture technology is an important and commonly used technique in cell biology research methods. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cell anabolism, cell growth and proliferation, etc. can be studied. Cell culture often requires transfer, which requires the use of a pipette.

[0003] Existing technology, such as publication number CN209352893U, provides a pipetting device for cell culture, including a lower shell, the bottom of which is connected to a nozzle, a first pull rod inserted in the lower shell, a piston fixed to the bottom of the first pull rod, semicircular limit plates fixed on both sides of the upper part of the lower shell, limit blocks fixed on both sides of the first pull rod, the limit blocks are arranged below the semicircular limit plates, a connecting plate is fixed above the first pull rod, a clamping mechanism is arranged in the connecting plate, an upper shell is fixedly connected above the lower shell, a second pull rod is inserted in the upper shell, the second pull rod is fixed in the middle above the connecting plate, and a limit plate is fixed on the top of the lower shell. Before using this utility model, the plug block needs to be rotated into the slot by the pull plate, and then the pull rod can be pulled. Pulling the pull rod drives the piston to move, so that the cell culture fluid can be adsorbed.

[0004] In this solution, the cell culture fluid is aspirated by pulling a rod to move the piston. However, in actual operation, the aspiration is driven by the rod. This means that to ensure the stability of the nozzle when aspirating the cell culture fluid, two hands are required to operate, which is not convenient for single-handed operation. In addition, when quantitative multiple aspiration and separation of samples are required, the amount of sample aspirated each time during manual aspiration varies to a certain extent, which is affected by the operator's operating proficiency. In view of this, we propose a pipetting device. Utility Model Content

[0005] The purpose of the utility model is to provide a pipetting device, which solves the problem that when quantitative multiple aspiration and separation of samples are required, the amount of samples aspirated each time during manual aspiration has certain differences and is affected by the operator's operating proficiency.

[0006] To achieve the above objectives, the present invention provides the following technical solutions:

[0007] A pipetting device comprises a pipette, the bottom of the pipette is connected to a suction head, the inner wall of the suction head is slidably connected to a piston, and the outer wall of the pipette is respectively provided with an adjustment component, an injection component, and an aspiration component, which are distributed at a 120-degree angle;

[0008] The adjustment component includes a slide, which is connected to the outer wall of the draw tube. The inner wall of the slide is connected to a magnetic slider. The inner wall of the draw tube is connected to a magnetic stopper, and the magnetic stopper is arc-shaped and fits the inner wall of the draw tube.

[0009] Preferably, the outer wall of the magnetic slider is connected to a push block, the surface of the push block is provided with anti-slip grooves, and the positions of the slideway on both sides of the magnetic slider are respectively provided with scale lines.

[0010] Preferably, the position of the scale line corresponding to the bottom of the piston is consistent with the position of the scale line corresponding to the bottom of the push block.

[0011] Preferably, the top of the pumping cylinder is connected to a support frame, and a telescopic cylinder is connected to the position between the support frame and the piston, and the telescopic cylinder is a four-stage telescopic setting.

[0012] Preferably, the injection assembly includes a first frame body, the first frame body is connected to the outer wall of the extraction tube, the inner wall of the first frame body is slidably connected to a first pressure strip, and the interior of the first frame body is connected to the interior of the telescopic tube through an air injection pipe.

[0013] Preferably, the suction assembly includes a second frame, the second frame is connected to the outer wall of the suction tube, the inner wall of the second frame is slidably connected to a second pressure strip, a pressure plate is provided on the outside of the second frame, and the second pressure strip is connected to the pressure plate, an exhaust hole is opened on the second frame, and the interior of the second frame is connected to the interior of the telescopic tube through an air suction pipe.

[0014] Preferably, the end of the air injection pipe connected to the first frame is located on a side of the first frame close to the extraction tube, and the end of the air suction pipe connected to the second frame is located on a side of the second frame away from the extraction tube.

[0015] By means of the above technical solution, the present invention provides a liquid transfer device having at least the following beneficial effects:

[0016] 1. The utility model provides an adjustment component to facilitate the quantitative extraction of cell culture fluid from the pumping tube, thereby ensuring the consistency of the amount of culture fluid extracted in multiple batches and facilitating quantitative distribution. Specifically, the push block is pushed to drive the magnetic slider to slide in the slideway, and the position of the corresponding scale line on the bottom of the push block is used to determine the amount of culture fluid required for a single time. When the magnetic slider slides, the magnetic stopper moves by its own magnetic traction to limit the piston. When aspirating the culture fluid, the piston slides on the inner wall of the pumping tube. When the piston slides and contacts the magnetic stopper, there is an obvious sense of obstruction, and at this time, the aspiration can be stopped.

[0017] 2. The utility model provides an injection component and a suction component in conjunction with a telescopic cylinder to pull the piston, so that the operator can perform suction and injection operations with one hand, freeing up the operator's other hand. During specific operations, when extraction is required, the operator holds the suction cylinder with the palm of his hand and presses the pressure plate with the fingers. When injecting culture fluid, the operator presses the first pressure strip with the fingers. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application:

[0019] Figure 1 This is a diagram showing the overall structure of the present utility model;

[0020] Figure 2 It is a partial cross-sectional view of the utility model;

[0021] Figure 3 It is a partial cross-sectional view of the injection assembly in the present invention;

[0022] Figure 4 It is a partial cross-sectional view of the suction component in the present invention.

[0023] In the figure: 1. suction cylinder; 11. suction head; 12. piston; 13. support frame; 14. telescopic cylinder; 2. adjustment assembly; 21. slideway; 211. scale line; 22. magnetic stopper; 23. magnetic slider; 24. push block; 3. injection assembly; 31. first frame; 32. first pressure strip; 33. gas injection pipe; 4. suction assembly; 41. second frame; 42. second pressure strip; 43. pressure plate; 44. suction pipe; 45. exhaust hole. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example 1

[0026] A pipetting device, such as Figure 1 、 Figure 2 As shown, it includes a suction tube 1, the bottom of the suction tube 1 is connected to a suction head 11, the inner wall of the suction head 11 is slidably connected to a piston 12, the outer wall of the suction tube 1 is respectively provided with an adjustment component 2, an injection component 3, and an absorption component 4, and they are distributed at one hundred and twenty degrees, wherein the adjustment component 2 includes a slide 21, the slide 21 is connected to the outer wall of the suction tube 1, the inner wall of the slide 21 is connected to a magnetic slider 23, the inner wall of the suction tube 1 is connected to a magnetic stopper 22, and the magnetic stopper 22 is arc-shaped and fits with the inner wall of the suction tube 1, the outer wall of the magnetic slider 23 is connected to a push block 24, the surface of the push block 24 is provided with anti-slip grooves, and the positions of the slide 21 on both sides of the magnetic slider 23 are respectively provided with scale lines 211, and the position of the scale line 211 at the bottom of the piston 12 is consistent with the position of the scale line 211 at the bottom of the push block 24.

[0027] In this embodiment, an adjustment component 2 is provided to facilitate the quantitative extraction of cell culture fluid by the pumping tube 1, so as to ensure the consistency of the amount of culture fluid extracted in multiple batches and facilitate quantitative distribution. Specifically, the push block 24 is pushed to drive the magnetic slider 23 to slide in the slide 21, and the amount of culture fluid required for a single time is determined by the position of the corresponding scale line 211 at the bottom of the push block 24. When the magnetic slider 23 slides, the magnetic stop block 22 moves through its own magnetic traction to limit the piston 12. When the culture fluid is aspirated, the piston 12 slides on the inner wall of the pumping tube 1. When the piston 12 slides to contact the magnetic stop block 22, there is an obvious sense of blockage. At this time, the aspiration can be stopped.

[0028] Example 2

[0029] like Figure 2 As shown, the top of the pumping cylinder 1 is connected to a support frame 13, and the position between the support frame 13 and the piston 12 is connected to a telescopic cylinder 14, which is a four-stage telescopic setting.

[0030] In this embodiment, a telescopic cylinder 14 that can be extended and retracted in multiple stages is provided to pull the piston 12 to move, thereby significantly shortening the length of the device compared to the pull rod pulling method in the comparative technology.

[0031] Example 3

[0032] like Figure 1 、 Figure 2 、 Figure 3 、 Figure 4As shown, the injection assembly 3 includes a first frame body 31, the first frame body 31 is connected to the outer wall of the extraction tube 1, the inner wall of the first frame body 31 is slidably connected to the first pressure strip 32, the interior of the first frame body 31 is connected to the interior of the telescopic tube 14 through the air injection pipe 33, the suction assembly 4 includes a second frame body 41, the second frame body 41 is connected to the outer wall of the extraction tube 1, the inner wall of the second frame body 41 is slidably connected to the second pressure strip 42, a pressure plate 43 is provided on the outside of the second frame body 41, and the second pressure strip 42 is connected to the pressure plate 43, an exhaust hole 45 is provided on the second frame body 41, the interior of the second frame body 41 is connected to the interior of the telescopic tube 14 through the air suction pipe 44, the end of the air injection pipe 33 connected to the first frame body 31 is on the side of the first frame body 31 close to the extraction tube 1, and the end of the air suction pipe 44 connected to the second frame body 41 is on the side of the second frame 41 away from the extraction tube 1.

[0033] In this embodiment, by setting the injection component 3 and the suction component 4 in cooperation with the telescopic cylinder 14 to pull the piston 12, the operator can perform the suction and injection operations with one hand, freeing the operator's other hand. During specific operations, when extraction is required, the palm of the hand holds the suction cylinder 1, and presses the pressure plate 43 with the fingers. The pressure plate 43 will press the second pressure strip 42 into the deep of the second frame 41, so that the second frame 41 generates negative pressure, so as to facilitate the suction of air from the telescopic cylinder 14 through the suction pipe 44, so that the telescopic cylinder 14 contracts and pulls the piston 12 to lift, so that suction is generated at the suction head 11, so as to facilitate the suction of culture fluid. When injecting culture fluid, the first pressure strip 32 is pressed by the fingers, so that the air in the first frame 31 is squeezed into the telescopic cylinder 14 through the injection pipe 33, so that the telescopic cylinder 14 is extended to drive the piston 12 to press down, so as to facilitate the discharge of culture fluid.

[0034] When the pipetting device of the present invention is in use, the amount of culture fluid required for a single time is determined by the position of the corresponding scale line 211 at the bottom of the push block 24. When the magnetic slider 23 slides, the magnetic stopper 22 is moved by its own magnetic traction to limit the piston 12. When the culture fluid is aspirated, the piston 12 slides on the inner wall of the suction tube 1. When the piston 12 slides to contact the magnetic stopper 22, there is an obvious sense of obstruction. At this time, the aspiration can be stopped. When it is necessary to aspirate, the hand grasps the suction tube 1 and presses the pressure plate 43 with the finger. The pressure plate 43 will press the second pressure strip 42 deep into the second frame 41, so that the second frame 41 generates negative pressure, so as to facilitate the suction of air in the telescopic cylinder 14 through the suction pipe 44, so that the telescopic cylinder 14 contracts and pulls the piston 12 to lift, so that suction is generated at the suction head 11 to facilitate the suction of culture fluid. When injecting culture fluid, the first pressure strip 32 is pressed by the finger, so that the air in the first frame 31 is squeezed into the telescopic cylinder 14 through the air injection pipe 33, so that the telescopic cylinder 14 extends and drives the piston 12 to press down, so as to facilitate the discharge of culture fluid.

[0035] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0036] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A pipetting device comprising a pipette (1), characterized in that: The bottom of the extraction tube (1) is connected to a suction head (11), the inner wall of the suction head (11) is slidably connected to a piston (12), and the outer wall of the extraction tube (1) is respectively provided with an adjustment component (2), an injection component (3), and a suction component (4), which are distributed at a 120-degree angle. The adjustment component (2) includes a slideway (21), the slideway (21) is connected to the outer wall of the draw tube (1), the inner wall of the slideway (21) is connected to a magnetic slider (23), the inner wall of the draw tube (1) is connected to a magnetic stopper (22), and the magnetic stopper (22) is arc-shaped and fits the inner wall of the draw tube (1).

2. A pipetting device according to claim 1, characterized in that: The outer wall of the magnetic slider (23) is connected to a push block (24), the surface of the push block (24) is provided with anti-slip grooves, and the positions of the slideway (21) on both sides of the magnetic slider (23) are respectively provided with scale lines (211).

3. A pipetting device according to claim 2, characterized in that: The position of the scale line (211) corresponding to the bottom of the piston (12) is consistent with the position of the scale line (211) corresponding to the bottom of the push block (24).

4. A pipetting device according to claim 1, characterized in that: The top of the pumping cylinder (1) is connected to a support frame (13), and a telescopic cylinder (14) is connected between the support frame (13) and the piston (12). The telescopic cylinder (14) is a four-stage telescopic arrangement.

5. A pipetting device according to claim 4, characterized in that: The injection assembly (3) comprises a first frame (31), the first frame (31) being connected to the outer wall of the extraction cylinder (1), the inner wall of the first frame (31) being slidably connected to a first pressure strip (32), and the interior of the first frame (31) being connected to the interior of the telescopic cylinder (14) via an air injection pipe (33).

6. A pipetting device according to claim 5, characterized in that: The suction assembly (4) includes a second frame (41), the second frame (41) is connected to the outer wall of the suction cylinder (1), the inner wall of the second frame (41) is slidably connected to a second pressure strip (42), a pressure plate (43) is provided outside the second frame (41), and the second pressure strip (42) is connected to the pressure plate (43), an exhaust hole (45) is opened on the second frame (41), and the interior of the second frame (41) is communicated with the interior of the telescopic cylinder (14) through an air suction pipe (44).

7. A liquid transfer device according to claim 6, characterized in that: One end of the air injection pipe (33) connected to the first frame (31) is located on a side of the first frame (31) close to the extraction cylinder (1), and one end of the air suction pipe (44) connected to the second frame (41) is located on a side of the second frame (41) away from the extraction cylinder (1).

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