Aerobic bacteria large-volume amplification device

Abstract

The utility model discloses a large-volume amplification device for aerobic bacteria, which relates to the technical field of large-volume culture of aerobic bacteria, and comprises a large-volume constant-temperature incubator, oxygenation equipment and a real-time bacterial concentration monitor for monitoring the bacterial concentration of a culture solution, the large-volume constant-temperature incubator comprises a box body, a transparent culture tank and a perforated plate, the perforated plate is arranged in the box body, the transparent culture tank is arranged on the perforated plate, and a culture solution is filled in the transparent culture tank; the oxygenation equipment comprises an air compressor and a nano air disc, the nano air disc is arranged at the bottom of the inner side of the transparent culture tank, an air tap is arranged on the nano air disc, the air compressor is communicated with the air tap through an air conveying hose, and the large-volume multiplication culture of aerobic bacteria can be realized.

Description

Technical Field

[0001] The utility model relates to the technical field of large-volume aerobic bacteria culture, in particular to a large-volume aerobic bacteria amplification device. Background Art

[0002] With the rapid increase in population and the acceleration of urbanization, the demand for civil infrastructure is also rapidly increasing. Construction land resources are extremely limited, necessitating the reinforcement of soils that do not meet foundation requirements. Traditional soil reinforcement methods generally utilize large-scale machinery and synthetic materials. Mechanical compaction consumes significant manpower and material resources, significantly disturbs the soil and damages its original structure, significantly impacts the surrounding environment, and is only practical within a limited depth range. Synthetic grouting materials generally include sodium silicate, epoxy resins, acrylates, phenolic resins, and polyurethanes. However, with the exception of sodium silicate, all other chemical grouts are toxic, posing a concerning environmental risk and threatening public health. In recent years, with the development and collaboration of interdisciplinary disciplines such as microbiology, geochemistry, and civil engineering, microbially induced calcium carbonate precipitation (MICP) has begun to be gradually applied to the construction industry. Because MICP is a biomineralization process that occurs widely in nature, it is environmentally friendly, simple in mechanism, and rapid and efficient, making it increasingly widely used in practical engineering. The mechanism of microbial induced calcium carbonate precipitation (MICP) is that urease, produced by microbial metabolism, hydrolyzes urea. The hydrolysis product reacts with calcium ions in the solution to form poorly soluble calcium carbonate. Applying this technology to soil reinforcement is not only environmentally friendly but also offers significant economic and social benefits. With further research and technological advancements, the application of MICP technology is expanding to areas such as environmental remediation, building material reinforcement, stone cultural relic restoration, biomedical applications, and agricultural soil improvement.

[0003] The microbial induced calcium carbonate precipitation (MICP) technology is commonly used for aerobic bacteria such as Sporosarcina pasteurii. It is usually cultured in a constant temperature shaking chamber. In addition to controlling the temperature, the dissolved oxygen content of the culture solution can also be changed by adjusting the oscillation frequency. About several hundred milliliters of aerobic bacterial culture can be cultured at a time, which can meet the needs of general laboratories. However, when the culture solution is amplified (for example, when more than 3L of culture solution is placed in a constant temperature shaking chamber at a time), the oscillation will cause the culture solution to splash out. At the same time, the oscillation method has limited effect on the improvement of the dissolved oxygen content of the culture solution, so that the dissolved oxygen content in the culture solution cannot meet the requirements for the reproduction of aerobic bacteria and inhibits the reproduction of aerobic bacteria. Ultimately, the bacterial concentration required for practical application cannot be achieved, and the culture efficiency is low. In addition, frequent sampling and testing of the bacterial solution concentration during the culture process is not only complicated to operate and has high requirements for experimental operators, but also easily causes contamination to the culture solution. However, in actual engineering applications, the construction site environment is simple and it is difficult to provide an environment suitable for the cultivation of aerobic bacteria. Furthermore, the amount of aerobic bacterial solution required is enormous, reaching dozens or even hundreds of times the amount required for a single laboratory culture. Therefore, a simple and practical aerobic bacteria large-volume amplification device is urgently needed. Based on this, a large-volume aerobic bacteria amplification device was designed to reduce the requirements for experimental operators, improve the efficiency of aerobic bacteria culture, and ultimately achieve large-volume aerobic bacteria amplification, thereby resolving the contradiction between the actual engineering demand for large-volume bacterial solution and the laboratory's ability to culture only a small amount of bacterial solution at a time. Utility Model Content

[0004] The purpose of the utility model is to overcome the shortcomings of the existing technology and provide a large-volume aerobic bacteria amplification device, which solves the traditional laboratory requirement for a specific temperature range for the cultivation of aerobic bacteria such as Bacillus pasteurianus, and simplifies the test of the bacterial concentration in the culture solution. The concentration of the bacterial solution in the culture solution can be obtained in real time, reducing the risk of the culture solution being contaminated by other bacteria when measuring the bacterial concentration of the culture solution.

[0005] The purpose of the utility model is achieved through the following technical solutions: a large-volume aerobic bacteria amplification device, which includes a large-volume constant-temperature incubator, an oxygenation device and a real-time bacteria concentration monitor for monitoring the bacterial concentration of the culture solution. The large-volume constant-temperature incubator includes a box body, a transparent culture tank and a porous plate, the porous plate is arranged in the box body, the transparent culture tank is arranged on the porous plate, and the transparent culture tank is filled with culture solution.

[0006] The oxygen enrichment equipment includes an air compressor and a nano gas disc, the nano gas disc is arranged at the inner bottom of the transparent culture tank, and the nano gas disc is provided with an air nozzle, and the air compressor is connected to the air nozzle through an air hose.

[0007] The real-time bacteria concentration monitor comprises a wavelength setting button, a probe and a bacteria concentration display screen. The probe is arranged on the outer wall of the transparent culture tank and monitors the bacteria concentration of the culture solution.

[0008] The box contains water and is equipped with a heating device and a control button for controlling the heating device. A temperature compensator and a temperature sensor are located below the porous plate. The temperature compensator absorbs vibrations, reducing the impact of water vibrations on the box, heating device, and temperature sensor. It also corrects for temperature variations in the water measured by the temperature sensor, ensuring accurate measurement results.

[0009] The box body is also provided with a drain pipe and a water inlet pipe. The drain pipe is arranged at the bottom of one side of the box body, and the water inlet pipe is arranged at the upper part of one side of the box body and is higher than the water level inside the box body.

[0010] A temperature display screen is provided on the outside of the box body, and the temperature display screen is connected to a temperature sensor. The temperature sensor transmits the detected water temperature to the temperature display screen for display, which is convenient for staff to observe.

[0011] The outer side of the box is also covered with a heat-insulating plastic layer to reduce heat loss and facilitate keeping aerobic bacteria in a constant temperature environment. The box is also provided with a handle to facilitate dragging by staff.

[0012] The oxygen enrichment equipment further comprises an air flow control knob for controlling the air flow rate, and a check valve is provided inside the air delivery hose to prevent backflow of air or oxygen.

[0013] The nano-gas disc is densely distributed with a number of tiny pores, which facilitate the air or oxygen from the oxygenation equipment to be better dissolved into the culture medium.

[0014] A one-way exhaust valve and a reserved hole for the air pipe are provided on the top of the box. The one-way exhaust valve facilitates the discharge of carbon dioxide produced by aerobic bacteria. One end of the air hose is connected to the air compressor, and the other end thereof passes through the reserved hole for the air pipe and is connected to the air nozzle.

[0015] The porous plate has a plurality of holes evenly distributed thereon, so as to facilitate the upward and downward flow of water 110 through the holes 116 .

[0016] The beneficial effects of the utility model are:

[0017] 1. The utility model utilizes a constant temperature water bath and oxygenation equipment to achieve precise control of aerobic bacteria culture temperature and dissolved oxygen content, so as to meet the requirements of different types of aerobic bacteria for different environmental temperatures and dissolved oxygen content.

[0018] 2. The real-time bacteria concentration monitor of the present invention simplifies the bacteria concentration test and greatly improves the culture efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural diagram of the utility model;

[0020] Figure 2 It is a schematic diagram of the cross-sectional structure of a large-volume constant temperature incubator;

[0021] Figure 3 It is a structural diagram of a real-time bacteria concentration monitor;

[0022] Figure 4 Schematic diagram of the structure of the porous plate;

[0023] Figure 5 Schematic diagram of the structure of the nano gas disk;

[0024] In the figure: 1-large volume constant temperature incubator, 2-oxygenation equipment, 3-real-time bacteria concentration monitor, 101-chamber, 102-temperature display screen, 103-control button, 104-one-way exhaust valve, 105-reserved hole for gas pipe, 106-handle, 107-drain pipe, 108-water inlet pipe, 109-power cord, 110-water, 111-transparent culture tank, 112-culture medium, 113-porous plate, 114-temperature compensator, 115-temperature sensor, 116-hole, 201-air compressor, 202-air flow control knob, 203-gas hose, 204-check valve, 205-compressor power cord, 206-nano gas disk, 207-fine air hole, 208-gas nozzle, 301-bacteria concentration display screen, 302-wavelength setting button, 303-probe, 304-data cable. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. The components of the embodiments of the present invention generally described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the utility model for protection, but merely represents selected embodiments of the present invention. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0027] Example 1:

[0028] like Figures 1 to 5 As shown, a large-volume aerobic bacterial amplification device includes a large-volume constant-temperature incubator 1 (1000mm×500mm×900mm), an aerator 2, and a real-time bacterial concentration monitor 3 for monitoring the bacterial concentration of a culture solution 112. The large-volume constant-temperature incubator 1 includes a housing 101, a transparent culture tank 111 (500mm×300mm×200mm), and a porous plate 113. The porous plate 113 is disposed within the housing 101, and the transparent culture tank 111 is disposed on the porous plate 113. The transparent culture tank 111 contains a culture solution 112. The large-volume constant-temperature incubator 1 is powered via a power cord 109, and the aerator 2 is powered via a compressor power cord 205.

[0029] The oxygenation device 2 includes an air compressor 201 and a nano-gas disc 206. The nano-gas disc 206 is arranged at the inner bottom of the transparent culture tank 111. The nano-gas disc 206 is immersed in the culture solution 112, and an air nozzle 208 is provided on the nano-gas disc 206. The air compressor 201 and the air nozzle 208 are connected through an air hose 203.

[0030] Turn on the large-volume constant-temperature incubator 1, the oxygenation device 2 and the real-time bacteria concentration monitor 3, preheat the large-volume constant-temperature incubator 1, and inject an appropriate amount of water 110 into the large-volume constant-temperature incubator 1. The injected water 110 can be water 110 that has been heated to a certain temperature, or water 110 at room temperature can be directly injected and then heated to a certain temperature by the heating device in the large-volume constant-temperature incubator 1; then prepare the culture solution 112 as required, open the upper cover of the large-volume constant-temperature incubator 1, and load the culture solution 112 into the transparent culture tank 111. First, set the bacteria detection wavelength of the real-time bacteria concentration monitor 3, and adjust the water level of the water 110 in the large-volume constant-temperature incubator 1 to make it higher than the water level of the culture solution 112 in the transparent culture tank 111. The aeration device 2 is positioned about 2 to 3 cm away from the nano-gas disc 206. A certain concentration of aerobic bacterial liquid is poured into the culture liquid 112 as required. The air compressor 201 of the aeration device 2 is turned on to release air from the nano-gas disc 206 into the culture liquid 112 through the air delivery hose 203 to provide oxygen for the aerobic bacteria. The culture is continued for a period of time until the OD value (or bacterial concentration value) displayed by the real-time bacterial concentration monitor 3 reaches the specified target value. After the bacterial culture is completed, the power supply of the large-volume constant temperature incubator 1, the aeration device 2, and the real-time bacterial concentration monitor 3 is turned off. The upper cover of the large-volume constant temperature incubator 1 is opened, and the transparent culture tank 111 is removed. After a period of culture, the culture liquid 112 in the transparent culture tank 111 has been converted into a bacterial liquid with the specified target bacterial concentration.

[0031] Example 2:

[0032] On the basis of Example 1, this embodiment makes an improvement to the real-time bacteria concentration monitor 3. The real-time bacteria concentration monitor 3 includes a wavelength setting button 302, a probe 303 and a bacteria concentration display screen 301. The probe 303 is set on the outer wall of the transparent culture tank 111 and monitors the bacteria concentration of the culture solution 112. The detected bacteria concentration information of the culture solution 112 is wirelessly transmitted to the real-time bacteria concentration monitor 3 in real time. The probe 303 is charged via a data cable. First, the required wavelength (generally 600nm) is set through the wavelength setting button 302 of the real-time bacteria concentration monitor 3. Then, the probe 303 of the real-time bacteria concentration monitor 3 is turned on and attached to the outer wall of the transparent culture tank 111 to monitor the aerobic bacteria concentration in the culture solution 112. The water level of the water 110 in the large-volume constant temperature incubator 1 is adjusted to be about 2 to 3 cm higher than the water level of the culture solution 112 in the transparent culture tank 111. A certain concentration of aerobic bacteria solution is poured into the culture solution 112 as required. The upper cover of the large-volume constant temperature incubator 1 is closed and opened. The air compressor 201 of the oxygenation device 2 releases air from the nano-gas disc 206 into the culture medium 112 through the air delivery hose 203. The culture is then continued for a period of time until the OD value (or bacterial concentration value) displayed on the bacterial concentration display screen 301 reaches the specified target value, indicating that a large-volume aerobic bacterial culture is completed. When the bacterial culture is completed, the water 110 in the large-volume constant temperature incubator 1 is drained. If the next batch of bacterial culture is to be continued, the water 110 in the large-volume constant temperature incubator 1 does not need to be drained, and fresh culture medium 112 can be directly replaced for the next bacterial culture.

[0033] Example 3:

[0034] Based on Example 1, this example improves the box 101. Water 110 is filled inside the box 101. The box 101 is provided with a heating device and a control button 103 for controlling the heating device. A temperature compensator 114 and a temperature sensor 115 are provided under the porous plate 113. The temperature compensator 114 and the temperature sensor 115 are both immersed in the water 110.

[0035] The housing 101 is also provided with a drain pipe 107 and a water inlet pipe 108. The drain pipe 107 is located at the bottom of one side of the housing 101, and the water inlet pipe 108 is located at the upper portion of one side of the housing 101, and is higher than the water level of the water 110 inside the housing 101. When bacterial culture is required, the water level of the water 110 in the large-volume constant-temperature incubator 1 is adjusted via the drain pipe 107 and the water inlet pipe 108 so that it is approximately 2 to 3 cm higher than the water level of the culture solution 112 in the transparent culture tank 111. When the bacterial culture is completed, the drain pipe 107 is opened and the water inlet pipe 108 is closed to drain the water 110 in the large-volume constant-temperature incubator 1. If further culture is to be continued, the water 110 in the large-volume constant-temperature incubator 1 does not need to be drained, and the next culture can be carried out.

[0036] A temperature display screen 102 is provided on the outside of the box body 101 , and the temperature display screen 102 is connected to a temperature sensor 115 . The temperature sensor 115 transmits the detected water temperature to the temperature display screen 102 for display, which is convenient for staff to observe.

[0037] The outer side of the box 101 is also covered with a heat-insulating plastic layer to reduce heat loss and keep the aerobic bacteria in a constant temperature environment. The box 101 is also provided with a handle 106 to facilitate dragging by staff. Turn on the large-volume constant-temperature incubator 1, the oxygen enrichment device 2 and the real-time bacteria concentration monitor 3, preheat the large-volume constant-temperature incubator 1, close the drain pipe 107 and open the water inlet pipe 108, inject an appropriate amount of water 110 into the large-volume constant-temperature incubator 1, turn on the heating device of the large-volume constant-temperature incubator 1, set the temperature of the water 110 to the target temperature through the control button 103, and turn on the temperature compensator 114 and the temperature sensor 115 at the same time. The heating device starts to heat the water 110 until the water temperature detected by the temperature sensor 115 reaches the target temperature and the temperature of the water 110 in the large-volume constant-temperature incubator 1 displayed on the temperature display screen 102 stabilizes to the set temperature level. At this time, the heating device stops heating, and the insulating plastic layer prevents heat loss, so that aerobic bacteria are in a nearly constant temperature environment, which is more conducive to bacterial cultivation. If the staff observes that the temperature of the water 110 in the large-volume constant-temperature incubator 1 displayed on the temperature display screen 102 is too low, they can also press the control button 103 again to control the heating device to heat.

[0038] Example 4:

[0039] Based on Example 1, this embodiment improves the oxygenation device 2. The oxygenation device 2 further includes an airflow control knob 202 for controlling the air flow rate. When the airflow control knob 202 is rotated clockwise, the rate at which the oxygenation device 2 delivers air or oxygen to the nano-gas disc 206 increases. When the airflow control knob 202 is rotated counterclockwise, the rate at which the oxygenation device 2 delivers air or oxygen to the nano-gas disc 206 decreases. A check valve 204 is provided inside the air delivery hose 203 to prevent backflow of air or oxygen.

[0040] The nano-gas disc 206 is densely distributed with a number of tiny pores 207 , which facilitate the air or oxygen from the oxygenation device 2 to be better dissolved into the culture solution 112 .

[0041] Example 5:

[0042] Based on Examples 1-4, this embodiment makes improvements to the box body 101 and the porous plate 113. A one-way exhaust valve 104 and a reserved hole 105 for the air supply pipe are provided on the top of the box body 101. The one-way exhaust valve 104 facilitates the discharge of carbon dioxide produced by aerobic bacteria. One end of the air supply hose 203 is connected to the air compressor 201, and the other end thereof passes through the reserved hole 105 for the air supply pipe and is connected to the air nozzle 208.

[0043] A plurality of holes 116 are evenly distributed on the porous plate 113 to facilitate the upward and downward flow of water 110 through the holes 116 .

[0044] The above description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Instead, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the concept described herein through the above teachings or techniques or knowledge in the relevant fields. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention are intended to be protected by the claims appended hereto.

Claims

1. A device for aerobic bacteria large volume amplification, characterized by: The invention comprises a large-volume constant-temperature incubator (1), an oxygenation device (2), and a real-time bacteria concentration monitor (3) for monitoring the bacteria concentration of a culture solution (112). The large-volume constant-temperature incubator (1) comprises a box body (101), a transparent culture tank (111), and a porous plate (113). The porous plate (113) is arranged in the box body (101), the transparent culture tank (111) is arranged on the porous plate (113), and the transparent culture tank (111) is filled with a culture solution (112). The oxygenation device (2) includes an air compressor (201) and a nano gas disc (206), wherein the nano gas disc (206) is arranged at the inner bottom of the transparent culture tank (111), and a gas nozzle (208) is provided on the nano gas disc (206), and the air compressor (201) and the gas nozzle (208) are connected through a gas hose (203).

2. The aerobic bacteria large volume amplification device according to claim 1, characterized in that: The real-time bacteria concentration monitor (3) comprises a wavelength setting button (302), a probe (303) and a bacteria concentration display screen (301). The probe (303) is arranged on the outer wall of the transparent culture tank (111) and monitors the bacteria concentration of the culture solution (112).

3. The aerobic bacteria large-volume amplification device according to claim 1, characterized in that: The box (101) is filled with water (110), the box (101) is provided with a heating device and a control button (103) for controlling the heating device, and a temperature compensator (114) and a temperature sensor (115) are provided below the porous plate (113).

4. The aerobic bacteria large-volume amplification device according to claim 3, characterized in that: The box (101) is also provided with a drainage pipe (107) and a water inlet pipe (108). The drainage pipe (107) is provided at the bottom of one side of the box (101), and the water inlet pipe (108) is provided at the upper part of one side of the box (101) and is higher than the water level of the water (110) inside the box (101).

5. The aerobic bacteria large volume amplification device according to claim 3, characterized in that: A temperature display screen (102) is provided on the outside of the box (101), and the temperature display screen (102) is connected to a temperature sensor (115).

6. The device for large-volume aerobic bacteria amplification according to any one of claims 3 to 5, characterized in that: The outer side of the box body (101) is covered with a heat-insulating plastic layer, and the box body (101) is also provided with a handle (106).

7. The aerobic bacteria large-volume amplification device according to claim 1, characterized in that: The oxygen enrichment device (2) further comprises an air flow control knob (202) for controlling the air flow rate, and a check valve (204) is provided inside the air delivery hose (203).

8. The aerobic bacteria large-volume amplification device according to claim 1 or 7, characterized in that: A plurality of fine pores (207) are densely distributed on the nano gas disk (206).

9. The aerobic bacteria large-volume amplification device according to claim 1, characterized in that: A one-way exhaust valve (104) and a reserved hole (105) for the air delivery pipe are provided on the top of the box (101); one end of the air delivery hose (203) is connected to the air compressor (201), and the other end thereof passes through the reserved hole (105) for the air delivery pipe and is connected to the air nozzle (208).

10. The aerobic bacteria large-volume amplification device according to claim 1, characterized in that: A plurality of holes (116) are evenly distributed on the porous plate (113).

Images