High-throughput immune cell clone culture robot

Abstract

The utility model discloses a high-flux immune cell clone culture robot, which belongs to the field of immune cell culture, and comprises a base, a material plate and a culture test tube, a robotic arm is fixed on one side of the top of the base, vertical plates are symmetrically arranged on the other side of the top of the base, a cavity is formed between the two vertical plates, and the material plate is fixed on the base. The material plates are uniformly arranged on the upper surfaces of the two vertical plates, through holes for placing culture test tubes are uniformly formed in the surfaces of the material plates, the culture test tubes are vertically arranged, lower extension plates are arranged below the two sides of each material plate, the two lower extension plates are respectively arranged on the outer sides of the two vertical plates, and the plurality of culture test tubes are mounted on the material plates. The material plates are evenly placed on the surface of the machine base, a plurality of test tubes are added one by one through the machine arm, so that labor intensity is reduced, meanwhile, when the material plates are installed, the stability of the relative positions of the material plates and the machine base can be guaranteed through the positioning bolts in the material plates, then positioning of the machine arm is facilitated, and accuracy during working is guaranteed.

Description

Technical Field

[0001] The utility model relates to the field of immune cell culture, and more specifically, to a high-throughput immune cell cloning culture robot. Background Art

[0002] Immune cells are cells involved in or associated with immune responses. They include lymphocytes, dendritic cells, monocytes / macrophages, granulocytes, and mast cells. Immune cells can be divided into several types, each of which plays an important role in the human body. Cultivating immune cells is a complex and critical technology, primarily used in fields such as immunotherapy and basic research. After isolation, immune cells must be cultured. The cells are placed in a culture medium and kept in an incubator at 37°C. Appropriate cytokines or nutrients are regularly added to stimulate their proliferation and activation.

[0003] However, when culturing immune cell clones, the single culture volume is huge. The existing technology adds cytokines one by one manually. This culture method is inefficient, and the repetitive work easily causes fatigue to the staff. Utility Model Content

[0004] 1. Technical problems to be solved

[0005] In response to the problems existing in the prior art, the purpose of the present invention is to provide a high-throughput immune cell cloning culture robot, which can realize the addition of multiple cell culture containers one by one through a robotic arm, and manual labor only needs to load and unload materials to reduce labor intensity.

[0006] 2. Technical solution

[0007] In order to solve the above problems, the present invention adopts the following technical solutions.

[0008] A high-throughput immune cell cloning and culture robot comprises a machine base, a material plate and a culture test tube. A robotic arm is fixed to one side of the top of the machine base, and a vertical plate is symmetrically arranged on the other side of the top of the machine base. A cavity is formed between the two vertical plates. The material plates are evenly arranged on the upper surfaces of the two vertical plates. Through holes for placing culture test tubes are evenly opened on the surface of the material plate. The culture test tubes are vertically arranged. Lower extension plates are provided below both sides of the material plate. The two lower extension plates are respectively placed on the outside of the two vertical plates. A handle is provided below the outer surface of the lower extension plate. A mounting hole is opened on the inner surface of the lower extension plate. A limiting bolt slides through the inside of the mounting hole. Positioning holes are evenly opened on the outer surface of the vertical plate. The end of the limiting bolt is adapted to the inner size of the positioning hole.

[0009] Furthermore, a connecting rod is provided at one end of the limit bolt away from the positioning hole, and the connecting rod passes through the outer surface of the lower extension plate. An I-shaped head is provided at one end of the connecting rod away from the limit bolt, and the I-shaped head is placed on the outer side of the lower extension plate.

[0010] Furthermore, a spring is sleeved on the surface of the connecting rod, the spring is placed inside the mounting hole, and one end of the spring is in contact with the limiting bolt.

[0011] Furthermore, support plates are symmetrically provided on the outer surface of the lower extension plate, and the support plates are placed under the I-head. A rotating shaft is rotatably installed between the two support plates, and a U-shaped plug-in rod is provided on the upper surface of the rotating shaft, and the U-shaped plug-in rod is stuck on the surface of the I-head.

[0012] Furthermore, a control pressure plate is provided on the lower surface of the rotating shaft, and the control pressure plate is placed on the inner side of the handle.

[0013] Furthermore, a control panel is embedded on one side of the front surface of the base, and an alarm is provided on one side of the upper surface of the base.

[0014] 3. Beneficial effects

[0015] Compared with the existing technology, the advantages of the present invention are: the present invention provides a high-throughput immune cell cloning culture robot, multiple culture tubes are installed on the material plate, and the material plate is evenly placed on the surface of the machine base, and multiple test tubes are added one by one by the robot arm to reduce labor intensity. At the same time, when the material plate is installed, the internal positioning bolts can be used to ensure the stability of the relative position of the material plate and the machine base, thereby facilitating the positioning of the robot arm to ensure accuracy during work. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the installation three-dimensional structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the material plate structure of the present utility model;

[0018] Figure 3 For the utility model Figure 2 Schematic diagram of the enlarged structure of area A;

[0019] Figure 4 It is a schematic diagram of the three-dimensional structure of the connecting rod of the present invention.

[0020] Explanation of the numbers in the figure: 1. Machine base; 101. Control panel; 102. Robot arm; 103. Alarm; 104. Vertical plate; 105. Positioning hole; 2. Material plate; 201. Lower extension plate; 202. Handle; 203. Mounting hole; 204. Limit bolt; 205. Connecting rod; 206. Spring; 207. I-head; 208. Support plate; 209. Rotating shaft; 210. U-shaped plug-in rod; 211. Control pressure plate; 3. Culture test tube. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0022] Example:

[0023] See also Figure 1-Figure 3 As shown, the high-throughput immune cell cloning and culture robot includes a base 1, a material plate 2 and a culture tube 3. A robot arm 102 is fixed to one side of the top of the base 1, and the robot arm 102 is used to process multiple culture tubes 3 one by one. A vertical plate 104 is symmetrically arranged on the other side of the top of the base 1. A cavity is formed between the two vertical plates 104 to prevent the material plate 2 from hitting the culture tube 3 during installation. The material plates 2 are evenly arranged on the upper surfaces of the two vertical plates 104. Through holes for placing the culture tube 3 are evenly opened on the surface of the material plate 2. The culture tube 3 is vertically arranged to achieve the same upper surface of multiple culture tubes 3 by installing or removing the material plate 2. For unloading, lower extension plates 201 are provided under both sides of the material plate 2. The two lower extension plates 201 are respectively placed on the outside of the two vertical plates 104 to improve the stability of the material plate 2. A handle 202 is provided under the outer surface of the lower extension plate 201 to facilitate the operation of the material plate 2. A mounting hole 203 is provided on the inner surface of the lower extension plate 201. A limiting bolt 204 slides through the inside of the mounting hole 203. Positioning holes 105 are evenly provided on the outer surface of the vertical plate 104. The end of the limiting bolt 204 is matched with the internal size of the positioning hole 105. The cooperation between the two can realize the fixation of the material plate 2 and the machine base 1, which facilitates the positioning of the robot arm 102.

[0024] Please refer to Figure 3 and Figure 4As shown, a connecting rod 205 is provided at one end of the limiting bolt 204 away from the positioning hole 105, and the connecting rod 205 passes through the outer surface of the lower extension plate 201, and an I-head 207 is provided at one end of the connecting rod 205 away from the limiting bolt 204, and the I-head 207 is placed on the outer side of the lower extension plate 201. A spring 206 is sleeved on the surface of the connecting rod 205, and the spring 206 is placed inside the mounting hole 203. One end of the spring 206 is in contact with the limiting bolt 204. The elastic force of the spring 206 is utilized to make the limiting bolt 204 automatically cooperate with the positioning hole 105 after the material plate 2 is installed in place, so as to realize the limiting work of the material plate 2. The end of the limiting bolt 204 can be set to a hemispherical shape to guide during installation.

[0025] Among them, the outer surface of the lower extension plate 201 is symmetrically provided with support plates 208, and the support plates 208 are placed under the I-head 207. A rotating shaft 209 is rotatably installed between the two support plates 208. A U-shaped plug-in rod 210 is provided on the upper surface of the rotating shaft 209. The U-shaped plug-in rod 210 is stuck on the surface of the I-head 207. A control pressure plate 211 is provided on the lower surface of the rotating shaft 209. The control pressure plate 211 is placed on the inner side of the handle 202. When disassembling the material plate 2, hold the handle 202 and press the control pressure plate 211 to drive the U-shaped plug-in rod 210 to pull out the limit bolt 204 to realize the disassembly work.

[0026] Please refer to Figure 1 A control panel 101 is embedded on one side of the front surface of the base 1, and an alarm 103 is provided on one side of the upper surface of the base 1.

[0027] Working principle: Place multiple culture tubes 3 on the surface of the material plate 2 respectively, and place multiple material plates 2 on the upper surface of the two vertical plates 104, and ensure that the culture tubes 3 are open upward. Due to the presence of the spring 206, the limit bolt 204 always has an inward force. After the material plate 2 is installed in place, it can be kept fixed by the cooperation between the limit bolt 204 and the positioning hole 105, so as to facilitate the precise positioning of the robot arm 102. The culture tubes 3 are added one by one through the robot arm 102. When the operation of a single material plate 2 is completed, the culture tubes 3 on another material plate 2 can be moved in and out. At this time, the material plate 2 after the work is completed can be replaced through the two handles 202. Hold the handle 202 and press the control pressure plate 211. At this time, the U-shaped pull-out rod 210 can be driven to rotate outward by the rotation of the rotating shaft 209, so as to drive the I-head 207 to move outward and disengage from the positioning hole 105. At this time, the material plate 2 can be lifted to achieve separation.

[0028] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any person skilled in the art who, within the technical scope disclosed in the present invention, makes equivalent substitutions or modifications based on the technical solutions and improved concepts of the present invention shall be covered by the scope of protection of the present invention.

Claims

1. A high-throughput immune cell cloning and culture robot, comprising a base (1), a material plate (2), and a culture tube (3), characterized in that: A machine arm (102) is fixed on one side of the top of the machine base (1), and a vertical plate (104) is symmetrically arranged on the other side of the top of the machine base (1), and a cavity is formed between the two vertical plates (104). The material plate (2) is evenly arranged on the upper surface of the two vertical plates (104), and the surface of the material plate (2) is evenly provided with through holes for placing culture tubes (3). The culture tubes (3) are vertically arranged. Lower extension plates (201) are arranged below both sides of the material plate (2). The two The lower extension plate (201) is respectively placed on the outside of the two vertical plates (104), a handle (202) is provided below the outer surface of the lower extension plate (201), a mounting hole (203) is provided on the inner surface of the lower extension plate (201), a limiting bolt (204) is slidably passed through the inside of the mounting hole (203), and positioning holes (105) are evenly provided on the outer surface of the vertical plate (104), and the end of the limiting bolt (204) and the inner size of the positioning hole (105) are adapted to each other.

2. The high-throughput immune cell cloning and culture robot according to claim 1, characterized in that: A connecting rod (205) is provided at one end of the limiting bolt (204) away from the positioning hole (105), and the connecting rod (205) passes through the outer surface of the lower extension plate (201). An I-shaped head (207) is provided at one end of the connecting rod (205) away from the limiting bolt (204), and the I-shaped head (207) is placed on the outer side of the lower extension plate (201).

3. The high-throughput immune cell cloning and culture robot according to claim 2, characterized in that: A spring (206) is sleeved on the surface of the connecting rod (205), and the spring (206) is placed inside the mounting hole (203), with one end of the spring (206) in contact with the limiting bolt (204).

4. The high-throughput immune cell cloning and culture robot according to claim 3, characterized in that: Support plates (208) are symmetrically arranged on the outer surface of the lower extension plate (201), and the support plates (208) are placed below the I-head (207). A rotating shaft (209) is rotatably installed between the two support plates (208), and a U-shaped plug-in rod (210) is arranged on the upper surface of the rotating shaft (209), and the U-shaped plug-in rod (210) is clamped on the surface of the I-head (207).

5. The high-throughput immune cell cloning and culture robot according to claim 4, characterized in that: A control pressing plate (211) is provided on the lower surface of the rotating shaft (209), and the control pressing plate (211) is placed inside the handle (202).

6. The high-throughput immune cell cloning and culture robot according to claim 1, characterized in that: A control panel (101) is embedded on one side of the front surface of the machine base (1), and an alarm (103) is provided on one side of the upper surface of the machine base (1).

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