Orchid tissue culture device

Abstract

The utility model relates to the technical field of pleione bulbocodioides tissue culture, and discloses an orchid tissue culture device which comprises a base, an L-shaped plate is fixedly installed on the side edge of the upper surface of the base, the top of the L-shaped plate is designed to be bent, and a plurality of supporting plates are horizontally fixed on the surface of the L-shaped plate and located above the base. And a plurality of placing grooves are formed in the upper surface of each supporting plate. According to the device, the base, the L-shaped plate, the multiple layers of supporting plates and the lifting assembly are arranged, the multiple culture bottles are placed on the surface of each supporting plate, the liquid storage boxes are arranged above each supporting plate, the multiple liquid drainage assemblies corresponding to the culture bottles are installed at the bottoms of the liquid storage boxes, and the multiple liquid storage boxes can be driven by the lifting assembly to ascend and descend synchronously; when the liquid storage box descends, the liquid inlet pipe at the top of the culture bottle is inserted into the liquid drainage assembly, so that the culture solution in the liquid storage box can enter the culture bottle along the liquid inlet pipe, the culture solution is added into all the culture bottles at a time, and the working efficiency is greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue culture of orchids, in particular to an orchidaceae plant tissue culture device. Background Art

[0002] The genus Tripterygium is a semi-epiphytic herbaceous plant of the Orchidaceae family. Its uniquely shaped and brightly colored flowers are deeply loved by people. It is an excellent ornamental plant for gardens and potted plants, and is also the source plant for the medicinal herb Tripterygium wilfordii. Tissue culture technology refers to the method of artificially cultivating living tissue. Specifically, the method involves removing a piece of tissue, an organ, or a cluster of cells from a multicellular organism and transplanting it into a nutrient-rich culture medium (usually made from serum and embryonic extract) to observe and study the growth, reproduction, and differentiation of cells and tissues.

[0003] Currently, existing tissue culture devices first place culture medium inside a culture bottle, then place the orchid on the medium, and then place the culture bottle on a multi-layered rack for cultivation. During the cultivation period, culture regulators (such as plant hormones, auxins, and cytokinins) need to be added to the culture bottle. This addition process requires manual removal of the top cover of the culture bottle one by one, which is time-consuming and affects work efficiency.

[0004] Therefore, the present invention proposes an orchidaceae plant tissue culture device in view of the above shortcomings. Utility Model Content

[0005] The purpose of the utility model is to solve the shortcomings of the prior art and to propose an orchidaceae plant tissue culture device.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a device for culturing orchid plants, comprising a base, an L-shaped plate fixedly installed on the side of the upper surface of the base, and the top of the L-shaped plate is bent, a plurality of support plates are horizontally fixed on the surface of the L-shaped plate and located above the base, a plurality of placement slots are opened on the upper surface of each support plate, and a culture bottle is arranged inside each placement slot, the culture bottle is provided with culture medium inside, and the top of the culture bottle is fixedly connected to a top cover, the middle of the top cover is fixedly connected to a liquid inlet pipe, a liquid storage box is provided above each support plate, a plurality of drainage components for injecting culture regulator into the culture bottle are fixed on the bottom wall of the liquid storage box, lifting components for driving the plurality of liquid storage boxes to move are installed on both sides of the top of the L-shaped plate, a liquid storage cylinder is fixed on one side of the upper surface of the base, and culture regulator is provided inside the liquid storage cylinder, a pump body connecting the liquid storage cylinder and the plurality of liquid storage boxes is installed on the top of the liquid storage cylinder, and a downward pressure component is fixed on the lower surface of the liquid storage box and located above each culture bottle.

[0007] As a further description of the above technical solution:

[0008] A plurality of universal wheels are fixedly mounted on the lower surface of the base.

[0009] As a further description of the above technical solution:

[0010] The lifting assembly includes an electric telescopic rod fixed to the top of the L-shaped plate, the bottom telescopic end of the electric telescopic rod is fixedly connected to a vertical rod, a plurality of cross rods are fixed to the surface of the vertical rod, and the plurality of cross rods are respectively fixedly connected to the outer walls of the plurality of liquid storage boxes.

[0011] As a further description of the above technical solution:

[0012] The drainage assembly includes a drainage pipe fixed to the bottom wall of the liquid storage box, a first ring plate and a second ring plate are fixed inside the drainage pipe, and the second ring plate is located above the first ring plate, a first spring is fixed to the lower surface of the second ring plate, and a blocking ball is fixedly connected to the bottom end of the first spring, and the blocking ball can be snapped into the circular hole in the middle of the first ring plate, an opening is provided on the side wall of the top end of the liquid inlet pipe, and the top end of the liquid inlet pipe can be inserted into the inside of the drainage pipe.

[0013] As a further description of the above technical solution:

[0014] A sealing ring is fixed on the inner side wall of the first ring plate, and the outer side wall of the liquid inlet pipe is in contact with the sealing ring.

[0015] As a further description of the above technical solution:

[0016] The pressing assembly includes a second spring fixed to the lower surface of the liquid storage box, the bottom end of the second spring is fixedly connected to an extrusion ring, and the second spring and the extrusion ring are both located outside the liquid discharge assembly.

[0017] As a further description of the above technical solution:

[0018] The liquid inlet of the pump body is communicated with the interior of the liquid storage cylinder through a pipeline, the liquid outlet of the pump body is communicated with multiple liquid storage boxes through pipelines, and a liquid filling port is provided on the top of the liquid storage cylinder.

[0019] The utility model has the following beneficial effects:

[0020] 1. In the utility model, a base, an L-shaped plate, a multi-layer support plate and a lifting assembly are provided. Multiple culture bottles are placed on the surface of each support plate. A liquid storage box is provided above each support plate. Multiple drainage assemblies corresponding to the culture bottles are installed at the bottom of the liquid storage box. The lifting assembly can drive multiple liquid storage boxes to rise and fall synchronously. When the liquid storage box descends, the liquid inlet pipe on the top of the culture bottle is inserted into the drainage assembly, so that the culture fluid in the liquid storage box can enter the interior of the culture bottle along the liquid inlet pipe, and the culture fluid is added to all the culture bottles at one time, which greatly improves work efficiency.

[0021] 2. In the present invention, by setting a downward pressure component, the downward pressure component can be pressed against the top of the liquid storage bottle, preventing the discharge component from driving the top cover and the culture bottle to rise synchronously when the lifting component drives the liquid storage box to rise, thereby ensuring that the liquid storage bottle is always located in the placement groove on the surface of the support plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is an overall schematic diagram of the utility model;

[0023] Figure 2 It is a partial three-dimensional diagram of the utility model;

[0024] Figure 3 For the utility model Figure 1 Enlarged view of point A in the middle.

[0025] Legend:

[0026] 1. Base; 2. L-shaped plate; 3. Support plate; 31. Placement slot; 4. Culture bottle; 5. Top cover; 6. Liquid inlet pipe; 61. Opening; 7. Liquid storage box; 8. Drain assembly; 81. Drain pipe; 82. First ring plate; 83. Second ring plate; 84. First spring; 85. Ball blocking; 9. Lifting assembly; 91. Electric telescopic rod; 92. Vertical rod; 93. Horizontal rod; 10. Liquid storage cylinder; 11. Pump body; 12. Universal wheel; 13. Second spring; 14. Extrusion ring. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0028] Reference Figure 1-Figure 3The utility model provides an embodiment of an orchid plant tissue culture device, comprising a base 1, an L-shaped plate 2 is fixedly installed on the side of the upper surface of the base 1, and the top of the L-shaped plate 2 is bent, and a plurality of support plates 3 are fixed horizontally on the surface of the L-shaped plate 2 and above the base 1, each support plate 3 has a plurality of placement grooves 31 on the upper surface, and each placement groove 31 has a culture bottle 4 inside, the culture bottle 4 is provided with a culture medium, and the top of the culture bottle 4 is fixedly connected to a top cover 5, and the middle of the top cover 5 is fixedly connected to an inlet Liquid tube 6, a liquid storage box 7 is provided above each support plate 3, and a plurality of drainage components 8 for injecting culture regulator into the culture bottle 4 are fixed to the bottom wall of the liquid storage box 7. Lifting components 9 for driving the movement of multiple liquid storage boxes 7 are installed on both sides of the top of the L-shaped plate 2. A liquid storage cylinder 10 is fixed on one side of the upper surface of the base 1, and the culture regulator is provided inside the liquid storage cylinder 10. A pump body 11 connecting the liquid storage cylinder 10 and multiple liquid storage boxes 7 is installed on the top of the liquid storage cylinder 10, and a downward pressure component is fixed on the lower surface of the liquid storage box 7 and located above each culture bottle 4.

[0029] In this embodiment, the top cover 5 is fixedly connected to the culture bottle 4 via threads, so the top cover 5 can be unscrewed from the top of the culture bottle 4 .

[0030] A plurality of universal wheels 12 are fixedly mounted on the lower surface of the base 1 .

[0031] After the universal wheel 12 is provided, it is convenient to move the device as a whole. In this embodiment, the universal wheel 12 is a universal wheel 12 with a locking structure. When the device is moved to a specified position, the universal wheel 12 can be locked and fixed.

[0032] The lifting assembly 9 includes an electric telescopic rod 91 fixed to the top of the L-shaped plate 2. The bottom telescopic end of the electric telescopic rod 91 is fixedly connected to a vertical rod 92. A plurality of cross bars 93 are fixed on the surface of the vertical rod 92. The plurality of cross bars 93 are respectively fixedly connected to the outer walls of the plurality of liquid storage boxes 7.

[0033] The extension and retraction of the electric telescopic rod 91 can drive the vertical rod 92 to move up and down, and the horizontal rod 93 on the surface of the vertical rod 92 can drive the liquid storage box 7 to move up and down.

[0034] The drainage assembly 8 includes a drainage pipe 81 fixed to the bottom wall of the liquid storage box 7, and a first ring plate 82 and a second ring plate 83 are fixed inside the drainage pipe 81, and the second ring plate 83 is located above the first ring plate 82, and a first spring 84 is fixed to the lower surface of the second ring plate 83, and a blocking ball 85 is fixedly connected to the bottom end of the first spring 84, and the blocking ball 85 can be snapped into the circular hole in the middle of the first ring plate 82. An opening 61 is provided on the side wall of the top end of the liquid inlet pipe 6, and the top end of the liquid inlet pipe 6 can be inserted into the inside of the drainage pipe 81.

[0035] When the liquid storage box 7 descends, the liquid inlet tube 6 on the top cover 5 of each culture bottle 4 will be inserted into the liquid discharge tube 81, and the liquid inlet tube 6 will push the blocking ball 85 upward, so that the blocking ball 85 will be separated from the circular hole in the middle of the first ring plate 82. At this time, the culture regulator inside the liquid storage box 7 can be discharged along the liquid discharge tube 81, and then enter the liquid inlet tube 6 along the opening 61 on the side wall of the liquid inlet tube 6, and finally enter the culture bottle 4, so as to achieve the simultaneous addition of culture regulator to all culture bottles 4.

[0036] When the liquid storage box 7 rises, the liquid inlet pipe 6 is separated downward from the liquid discharge pipe 81, and the first spring 84 presses the blocking ball 85 downward, so that the blocking ball 85 blocks the circular hole in the middle of the first ring plate 82 again, thereby closing the liquid discharge pipe 81.

[0037] A sealing ring is fixed to the inner wall of the first ring plate 82 , and the outer wall of the liquid inlet pipe 6 is in contact with the sealing ring.

[0038] During the process of the liquid inlet tube 6 being inserted into and separated from the liquid discharge tube 81 , the sealing ring can play a sealing role to prevent the culture regulator from leaking too much from the gap between the liquid inlet tube 6 and the first ring plate 82 .

[0039] The pressing assembly includes a second spring 13 fixed to the lower surface of the liquid storage box 7 , and a squeezing ring 14 is fixedly connected to the bottom end of the second spring 13 . Both the second spring 13 and the squeezing ring 14 are located outside the liquid discharge assembly 8 .

[0040] When the liquid storage box 7 descends, the squeezing ring 14 first fits against the top cover 5 on the top of the culture bottle 4 under the squeezing force of the second spring 13. When the liquid storage box 7 rises, the second spring 13 still pushes the squeezing ring 14, so that the squeezing ring 14 presses the culture bottle 4 tightly into the placement groove 31, preventing the culture bottle 4 from rising synchronously with the drainage component 8.

[0041] The liquid inlet of the pump body 11 is communicated with the interior of the liquid storage cylinder 10 through a pipeline, and the liquid outlet of the pump body 11 is communicated with multiple liquid storage boxes 7 through pipelines. A liquid filling port is provided on the top of the liquid storage cylinder 10.

[0042] In this embodiment, the liquid storage cylinder 10 and the liquid storage box 7 are both made of transparent material, so that the remaining amount of culture regulator in the liquid storage cylinder 10 and the liquid storage box 7 can be observed. When the culture regulator in the liquid storage box 7 is insufficient, the pump body 11 can be activated to pump the culture regulator in the liquid storage cylinder 10 into the multiple liquid storage boxes 7.

[0043] Working principle: first add culture medium to the inside of the culture bottle 4, then insert the garlic orchid into the culture medium, then screw on the top cover 5 on the top of the culture bottle 4. After the garlic orchid is placed inside all the culture bottles 4, the culture bottle 4 is placed in the placement groove 31 on the surface of the support plate 3. When it is necessary to add culture regulator to the inside of the culture bottle 4, start the electric telescopic rod 91 to drive the multiple liquid storage boxes 7 to descend, so that the liquid inlet pipe 6 at the top of the culture bottle 4 is inserted into the corresponding drainage pipe 81 above, and the culture regulator in the liquid storage box 7 can enter the culture bottle 4 along the drainage pipe 81 and the liquid inlet pipe 6. After the addition is completed, the electric telescopic rod 91 drives the multiple liquid storage boxes 7 to rise and reset.

[0044] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An orchidaceae plant tissue culture device, characterized in that: The invention comprises a base (1), wherein an L-shaped plate (2) is fixedly installed on the side of the upper surface of the base (1), and the top of the L-shaped plate (2) is bent, and a plurality of support plates (3) are horizontally fixed on the surface of the L-shaped plate (2) and located above the base (1), and a plurality of placement grooves (31) are opened on the upper surface of each support plate (3), and a culture bottle (4) is arranged inside each placement groove (31), and a culture medium is arranged inside the culture bottle (4), and a top cover (5) is fixedly connected to the top of the culture bottle (4), and a liquid inlet pipe (6) is fixedly connected to the middle of the top cover (5), and each support plate (3) is provided above the support plate (3). Each of the liquid storage boxes (7) is provided with a plurality of liquid discharge components (8) for injecting culture regulators into the culture bottles (4) fixed on the bottom wall of the liquid storage box (7); a lifting component (9) for driving the plurality of liquid storage boxes (7) to move is installed on both sides of the top of the L-shaped plate (2); a liquid storage cylinder (10) is fixed on one side of the upper surface of the base (1), and the liquid storage cylinder (10) is provided with culture regulators inside; a pump body (11) connecting the liquid storage cylinder (10) and the plurality of liquid storage boxes (7) is installed on the top of the liquid storage cylinder (10); and a downward pressure component is fixed on the lower surface of the liquid storage box (7) and located above each culture bottle (4).

2. The orchidaceae plant tissue culture device according to claim 1, characterized in that: A plurality of universal wheels (12) are fixedly mounted on the lower surface of the base (1).

3. The orchidaceae plant tissue culture device according to claim 1, characterized in that: The lifting assembly (9) comprises an electric telescopic rod (91) fixed to the top of the L-shaped plate (2); the telescopic end at the bottom of the electric telescopic rod (91) is fixedly connected to a vertical rod (92); a plurality of cross rods (93) are fixed to the surface of the vertical rod (92); and the plurality of cross rods (93) are respectively fixedly connected to the outer walls of the plurality of liquid storage boxes (7).

4. The orchidaceae plant tissue culture device according to claim 1, characterized in that: The liquid discharge assembly (8) includes a liquid discharge pipe (81) fixed to the bottom wall of the liquid storage box (7), a first ring plate (82) and a second ring plate (83) are fixed inside the liquid discharge pipe (81), and the second ring plate (83) is located above the first ring plate (82), a first spring (84) is fixed to the lower surface of the second ring plate (83), and a blocking ball (85) is fixedly connected to the bottom end of the first spring (84), and the blocking ball (85) can be snapped into the circular hole in the middle of the first ring plate (82), and an opening (61) is opened on the side wall of the top end of the liquid inlet pipe (6), and the top end of the liquid inlet pipe (6) can be inserted into the inside of the liquid discharge pipe (81).

5. The orchidaceae plant tissue culture device according to claim 4, characterized in that: A sealing ring is fixed to the inner wall of the first ring plate (82), and the outer wall of the liquid inlet pipe (6) is in contact with the sealing ring.

6. The orchidaceae plant tissue culture device according to claim 1, characterized in that: The downward pressure assembly includes a second spring (13) fixed to the lower surface of the liquid storage box (7), the bottom end of the second spring (13) is fixedly connected to an extrusion ring (14), and the second spring (13) and the extrusion ring (14) are both located outside the liquid discharge assembly (8).

7. The orchidaceae plant tissue culture device according to claim 1, characterized in that: The liquid inlet of the pump body (11) is connected to the interior of the liquid storage cylinder (10) through a pipeline, and the liquid outlet of the pump body (11) is connected to multiple liquid storage boxes (7) through pipelines. A liquid filling port is provided on the top of the liquid storage cylinder (10).

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