Urine exfoliated cell collecting device

Abstract

The utility model provides a urine cast-off cell collecting device and belongs to the field of samplers. The urine exfoliated cell collecting device comprises a specimen container, a connector and a cell collecting filter screen, the specimen container comprises a container body and a connecting pipe section communicated with an inner cavity of the container body, the cell collecting filter screen is arranged in the connector, and the connector is detachably and fixedly connected with the connecting pipe section. At least part of the side wall of the container body is a deformation wall which can elastically deform under the action of external force so as to compress the volume of the inner cavity of the container body. According to the utility model, the urine sample is collected by utilizing the sample container which can deform under external force so as to compress the volume of the inner cavity, so that the urine sample is pressed in the process of filtering the urine sample so as to accelerate the urine sample to pass through the cell collection filter screen, and the collection efficiency of cast-off cells is improved.

Description

Technical Field

[0001] The utility model belongs to the field of samplers, in particular to a urine exfoliated cell collection device. Background Art

[0002] Bladder cancer can be diagnosed through urine cytology. Compared to pathological biopsies and invasive cystoscopy, urine cytology is non-invasive and has important clinical application value in the prediction of bladder cancer. Urine cytology primarily measures the number of cells in the urine and the presence of abnormal cells or cell changes to obtain information about the patient's health status.

[0003] Since there are fewer exfoliated cells per unit volume of urine, a large amount of urine needs to be collected, and sometimes multiple urinations are required. Exfoliated cells are generally collected by centrifugal sedimentation. The centrifugal sedimentation method uses a centrifuge to deposit the exfoliated cells in the urine in a sedimentation tube. The operator uses a pipette to suck out the supernatant in the sedimentation tube, so that the exfoliated cells are suspended in the remaining small amount of urine. This method requires a specially configured large-capacity, low-speed centrifuge, which is cumbersome to operate, expensive, and has poor flexibility in use. Moreover, this method is generally only suitable for situations where the specimen volume is small and the cell content is high. It is difficult to conveniently handle specimens with large specimens and low cell content.

[0004] The Chinese invention patent application with the existing application publication number CN111826270A discloses a device for conveniently collecting exfoliated cells from a large amount of body fluids. The device includes a specimen container, a liquid inlet chamber, a liquid discharge chamber, and a cell collection bottle. The liquid inlet chamber is connected to the mouth of the specimen container, the liquid discharge chamber is connected to the liquid inlet chamber by a thread, and a cell collection filter membrane is fixed on the liquid discharge chamber. When in use, a urine specimen is added to the specimen container, and then the liquid inlet and discharge chambers are connected to the liquid inlet chamber. The specimen container is inverted so that the urine passes through the cell collection filter membrane under its own gravity and is discharged, while the cells in the urine remain on the cell collection filter membrane. After the urine is filtered, the liquid discharge chamber is removed from the liquid inlet chamber and installed on the cell collection bottle. The side of the cell collection filter membrane with exfoliated cells is immersed in the preservation solution in the cell collection bottle, and the exfoliated cells are eluted into the preservation solution by shaking or ultrasound.

[0005] The device disclosed in this invention patent is simple to operate, low-cost, and flexible to use, capable of processing a large number of specimens. However, the device uses gravity to filter urine to remove detached cells. Under the action of gravity, urine passes through the cell collection filter at a slow rate. Furthermore, as filtration progresses, the amount of urine above the cell collection filter decreases, further slowing the rate at which urine passes through the cell collection filter. This results in a lengthy filtration process. Utility Model Content

[0006] The purpose of the utility model is to provide a urine exfoliated cell collection device to solve the technical problem in the prior art that it takes a long time to filter the exfoliated cells in urine by relying on the gravity of the urine itself.

[0007] To achieve the above objectives, the technical solution of the urine exfoliated cell collection device provided by the present invention is:

[0008] A urine exfoliated cell collection device includes a specimen container, a connector and a cell collection filter. The specimen container includes a container body and a connecting pipe section connected to the inner cavity of the container body. The cell collection filter is arranged in the connector. The connector and the connecting pipe section are detachably fixedly connected. At least part of the side wall of the container body is a deformable wall that can undergo elastic deformation under the action of external force to compress the volume of the inner cavity of the container body.

[0009] As a further improvement, the bottom wall of the container body is a force-bearing wall for bearing the pressing force perpendicular to the bottom wall, and the circumferential side walls of the container body are all wavy structures. The circumferential side walls of the container body can fold when subjected to a force perpendicular to the bottom wall, and the circumferential side walls of the container body constitute deformation walls.

[0010] As a further improvement, the circumferential side walls of the container body are load-bearing walls for bearing the extrusion force from the outside to the inside, and the circumferential side walls of the container body are all deformed walls that become concave when subjected to the extrusion force from the outside to the inside.

[0011] As a further improvement, the connector includes a large diameter section and a small diameter section. The inner diameter of the large diameter section is larger than the inner diameter of the small diameter section. An internal step structure is formed at the junction of the inner wall surfaces of the large diameter section and the small diameter section. The connecting pipe section extends into the large diameter section and is fixedly connected to the large diameter section. The cell collection filter is pressed between the axial end face of the connecting pipe section and the step surface of the internal step structure.

[0012] As a further improvement, the connecting pipe section is threadedly connected to the large diameter section of the connecting head.

[0013] As a further improvement, the urine exfoliated cell collection device also includes a waste liquid bottle, the upper end of which is provided with a waste liquid inlet for the filtered urine to enter, and a support structure for supporting the connector or specimen container is provided at the waste liquid inlet.

[0014] As a further improvement, the connector includes a large diameter section and a small diameter section. The outer diameter of the large diameter section is larger than the outer diameter of the small diameter section. An outer step structure is formed at the junction of the outer wall surfaces of the large diameter section and the small diameter section. The large diameter section is connected to the connecting pipe section. The inner diameter of the waste liquid inlet is larger than the outer diameter of the small diameter section and smaller than the outer diameter of the large diameter section. The upper end of the waste liquid bottle is used to support the step surface of the outer step structure after the small diameter section is inserted into the waste liquid inlet. The upper end of the waste liquid bottle constitutes a support structure.

[0015] As a further improvement, the inner diameter of the waste liquid inlet is larger than the outer diameter of the large diameter section and smaller than the outer diameter of the container body. The upper end of the waste liquid bottle is used to support the side wall of the container body where the connecting pipe section is located after the connecting head passes through the waste liquid inlet. The upper end of the waste liquid bottle constitutes a supporting structure.

[0016] As a further improvement, the connector includes a large diameter section and a small diameter section. The inner diameter of the large diameter section is larger than the inner diameter of the small diameter section. An internal step structure is formed at the junction of the inner wall surfaces of the large diameter section and the small diameter section. The large diameter section is connected to the connecting mouth. The waste liquid inlet is a tubular mouth, which extends into the large diameter section and is fixedly connected to the large diameter section. The cell collection filter is pressed between the axial end face of the tubular mouth and the step surface of the internal step structure.

[0017] As a further improvement, the urine exfoliated cell collection device also includes a bracket, which includes a base, a vertical frame fixed on the base, and a suspension fixed on the vertical frame. A support ring is provided on the suspension. The inner diameter of the support ring is larger than the outer diameter of the large diameter section and smaller than the outer diameter of the container body. The support ring is used to support the side wall of the container body where the connecting pipe section is located after the connecting head passes through.

[0018] The beneficial effect is that the urine exfoliated cell collection device provided by the utility model is an improvement over the prior art. The urine exfoliated cell collection device uses a specimen container with a deformable wall to collect urine specimens. During the filtering process of the urine specimen, the operator can apply force to the specimen container by pressing or squeezing to elastically deform the deformable wall, thereby compressing the volume of the inner cavity of the container body. The urine specimen in the specimen container is accelerated by the pressure to pass through the cell collection filter, thereby improving the collection efficiency of the exfoliated cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is an exploded view of Example 1 of the urine exfoliated cell collection device of the present invention;

[0020] Figure 2 This is a schematic structural diagram of the connector in Example 1 of the urine exfoliated cell collection device of the present invention.

[0021] Description of reference numerals:

[0022] 1. Specimen container; 11. Container body; 12. Connecting pipe section; 2. Connector; 21. Large diameter section; 22. Small diameter section; 23. Inner step structure; 24. Outer step structure; 3. Cell collection filter; 4. Waste liquid bottle; 41. Bottle body; 42. Tubular mouth. DETAILED DESCRIPTION

[0023] The present invention is further described in detail below with reference to the embodiments.

[0024] In order to solve the problems in the existing technology, the basic concept of the present invention is to use a specimen container that can be deformed under external force to compress the inner cavity volume to collect urine specimens, so as to apply pressure to the urine specimen during the filtration process to accelerate the urine specimen through the cell collection filter and improve the collection efficiency of exfoliated cells.

[0025] Specific embodiment 1 of the urine exfoliated cell collection device provided by the present invention:

[0026] A urine exfoliated cell collection device, see attached Figure 1 , including a specimen container 1, a connector 2, a cell collection filter 3 and a waste liquid bottle 4. The cross sections of the specimen container 1, the connector 2 and the waste liquid bottle 4 are all circular, and the cell collection filter 3 is disc-shaped.

[0027] The specimen container 1 comprises a container body 11 and a connecting tube section 12. The connecting tube section 12 is located at one axial end of the container body 11. The outer diameter of the connecting tube section 12 is smaller than that of the specimen container 1 and the connecting tube section 12 communicates with the inner cavity of the container body 11. The circumferential sidewalls of the container body 11 are all wavy in structure and can fold axially when subjected to an axial force. After folding, the axial dimension of the container body 11 is reduced. The bottom wall of the container body 11 is a load-bearing wall for the operator to apply circumferential force to the container body 11 by pressing. The bottom wall of the container body 11 is the end wall of the container body 11 away from the connecting tube section 12.

[0028] See attached Figure 2 The connector 2 includes a large diameter section 21 and a small diameter section 22, wherein the inner diameter of the large diameter section 21 is larger than the inner diameter of the small diameter section 22, and an inner step structure 23 is formed at the junction of the inner wall surfaces of the large diameter section 21 and the small diameter section 22; the outer diameter of the large diameter section 21 is larger than the outer diameter of the small diameter section 22, and an outer step structure 24 is formed at the junction of the outer wall surfaces of the large diameter section 21 and the small diameter section 22.

[0029] The inner wall of the large diameter section 21 is formed with an internal thread, and the outer peripheral wall of the connecting pipe section 12 is formed with an external thread. When in use, the connecting pipe section 12 can be installed into the large diameter section 21 by screwing, and a good sealing effect can be achieved. In other embodiments, the connecting pipe section 12 and the large diameter section 21 can also be assembled together by an interference fit, and can also be well sealed. For this connection method, the cross-sections of the specimen container 1, the connector 2 and the waste liquid bottle 4 can be set to a square shape, and the cell collection filter 3 can be set to a square sheet structure.

[0030] Before connecting the connector 2 to the specimen container 1, the cell collection filter 3 must be placed on the step surface of the inner step structure 23; after connecting the connector 2 to the specimen container 1, the edge of the cell collection filter 3 is pressed between the axial end face of the connecting pipe section 12 and the step surface of the inner step structure 23.

[0031] The waste liquid bottle 4 includes a bottle body 41 and a waste liquid inlet located at the upper end of the bottle body 41. In this embodiment, the waste liquid inlet is a tubular opening 42. The inner diameter of the tubular opening 42 is larger than the outer diameter of the small-diameter section 22 of the connector 2, but the inner diameter of the tubular opening 42 is smaller than the outer diameter of the small-diameter section 22 of the connector 2. Therefore, after the small-diameter section 22 of the connector 2 is inserted into the tubular opening 42, the upper end surface of the tubular opening 42 will abut against the stepped surface of the outer step structure 24 of the connector 2, thereby supporting the connector 2.

[0032] The method for collecting exfoliated cells in a urine specimen by the urine exfoliated cell collection device is as follows:

[0033] First, place the connecting pipe section 12 of the specimen container 1 upwards, and add the urine specimen for exfoliated cell collection into the specimen container 1 through the connecting pipe section 12;

[0034] Next, the cell collection filter 3 is placed into the connector 2, and then the connector 2 is connected to the specimen container 1;

[0035] Then, turn the specimen container 1 over so that the connecting pipe section 12 faces downward, and then insert the small-diameter section 22 of the connecting pipe section 12 into the tubular opening 42 of the waste liquid bottle 4. At this time, the waste liquid bottle 4 is located below the connector 2, and the specimen container 1 is located above the connector 2.

[0036] The operator presses the bottom wall of the container body 11, and the circumferential side wall of the container body 11 folds along the axial direction, thereby reducing the volume of the inner cavity of the container body 11, generating pressure on the urine specimen inside, accelerating the speed at which the urine specimen passes through the cell collection filter 3, and shortening the detection cycle;

[0037] After all the urine specimens in the specimen container 1 are filtered and flow into the waste liquid bottle 4, remove the specimen container 1 and the connector 2, and then remove the connector 2 from the specimen container 1;

[0038] The cell collection filter 3 is taken out, and the side of the cell collection filter 3 with cells is completely immersed in the cell preservation solution. The cells collected on the cell collection filter 3 are eluted into the cell preservation solution by shaking or ultrasonic method for subsequent detection and analysis.

[0039] In the above process, after the circumferential side wall of the container body 11 is folded, the volume of the inner cavity of the specimen container 1 can be compressed. During the folding process of the container body 11, the circumferential side wall thereof undergoes elastic deformation, and thus the circumferential side wall of the container body 11 constitutes a deformed wall.

[0040] In other embodiments, the small diameter section of the connector can also be fixedly connected to the tubular opening of the waste liquid bottle by means of threads, but in this case, a vent hole for the internal gas to be discharged needs to be opened on the waste liquid bottle so that the filtration can proceed smoothly.

[0041] Specific embodiment 2 of the urine exfoliated cell collection device provided by the present invention:

[0042] This embodiment is based on Example 1, and the difference from Example 1 is that the circumferential side wall of the container body in this embodiment is a load-bearing wall, which is used to withstand the extrusion force from the outside to the inside. After being subjected to the extrusion force, the circumferential side wall of the container body can undergo an inward concave elastic deformation. Therefore, the circumferential side wall of the container body in this embodiment is a deformable wall.

[0043] In the process of pressurized filtration of the urine specimen in this embodiment, the operator can hold the circumferential side wall of the container body with both hands and squeeze the circumferential side wall of the container body.

[0044] In other embodiments, a one-way air valve can be provided on the end wall of the container body away from the connecting pipe section. After the operator squeezes the container body once, he releases the container body, and the container body returns to its original state. External air can enter the container body through the one-way air valve, making it easier to squeeze the container body again. In this way, the operator does not need to squeeze the entire urine specimen at once, but can squeeze and filter it in multiple times, which is convenient for operation.

[0045] In other embodiments, the two end walls of the container body in the axial direction can also be deformed to facilitate the operator to squeeze the specimen container more conveniently. In this embodiment, all side walls of the container body are deformable walls.

[0046] Specific embodiment 3 of the urine exfoliated cell collection device provided by the present invention:

[0047] This embodiment is based on Example 1, and differs from Example 1 in that the cell collection filter in this embodiment is fixedly connected to one axial end of the connector. Therefore, the connector in this embodiment does not need to be set in a stepped shape, and can be a structure of equal diameter. The outer side of the connector is formed with an external thread, and the inner side of the connecting pipe section is formed with an internal thread for installing the connector.

[0048] After the urine sample is filtered using the urine exfoliated cell collection device of this embodiment, the exfoliated cells are collected on the outward side of the cell collection filter, making it easier for the operator to collect the exfoliated cells.

[0049] Specific embodiment 4 of the urine exfoliated cell collection device provided by the present invention:

[0050] This embodiment is based on embodiment 1, and differs from embodiment 1 in that the urine exfoliated cell collection device in this embodiment does not have a waste liquid bottle.

[0051] The urine exfoliated cell collection device in this embodiment needs to be configured with a special bracket, which includes a base, a vertical frame fixed on the base, and a suspension fixed on the vertical frame and in a cantilevered state. A support ring is provided on the suspension. The inner diameter of the support ring is larger than the outer diameter of the large diameter section and smaller than the outer diameter of the container body. The support ring is used to support the side wall of the container body where the connecting pipe section is located after the connecting head passes through.

[0052] During use, the specimen container containing the urine specimen is placed upside down on the support ring, and a collection tank is placed under the support ring to collect waste liquid.

[0053] Specific embodiment 5 of the urine exfoliated cell collection device provided by the present invention:

[0054] This embodiment is based on Example 1, and the difference from Example 1 is that the inner diameter of the tubular opening in this embodiment is larger than the outer diameter of the large diameter section and smaller than the outer diameter of the container body. The upper end of the waste liquid bottle is used to support the side wall of the container body where the connecting pipe section is located after the connecting head passes through the waste liquid inlet. The upper end of the waste liquid bottle constitutes a supporting structure.

[0055] In other embodiments, the waste liquid inlet of the waste liquid bottle may not be a tubular opening. In this embodiment, the waste liquid bottle has an upper end wall at the upper end of the bottle body, and a circular hole is formed in the center of the upper end wall, which constitutes the waste liquid inlet. After the connector is inserted into the waste liquid inlet, the upper end wall of the waste liquid bottle body abuts against the side wall of the container body where the connecting tube section is located, thereby supporting the specimen container.

[0056] Specific embodiment 6 of the urine exfoliated cell collection device provided by the present invention:

[0057] This embodiment is based on Example 1 and differs from Example 1 in that the connector in this embodiment also includes a large-diameter section and a small-diameter section, but the large-diameter section and the small-diameter section are oriented in opposite directions. Specifically, the connecting tube section is internally threaded and fixedly connected to the small-diameter section, while the tubular opening of the waste liquid bottle is externally threaded and threadedly connected to the large-diameter section. The tubular opening extends into the large-diameter section, and the axial end wall of the tubular opening presses the cell collection filter against the stepped surface of the inner step structure.

[0058] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments without inventive effort, or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A urine exfoliated cell collection device, comprising a specimen container, a connector, and a cell collection filter, wherein the specimen container comprises a container body and a connecting pipe section communicating with the inner cavity of the container body, the cell collection filter is disposed within the connector, and the connector and the connecting pipe section are detachably fixedly connected, wherein: At least part of the side wall of the container body is a deformable wall that can be elastically deformed under the action of external force to compress the volume of the inner cavity of the container body.

2. The urine exfoliated cell collection device according to claim 1, characterized in that: The bottom wall of the container body is a load-bearing wall used to withstand the pressing force perpendicular to the bottom wall. The circumferential side walls of the container body are all wavy structures. The circumferential side walls of the container body can fold when subjected to a force perpendicular to the bottom wall. The circumferential side walls of the container body constitute deformation walls.

3. The urine exfoliated cell collection device according to claim 1, characterized in that: The circumferential side walls of the container body are load-bearing walls for bearing the extrusion force from the outside to the inside. The circumferential side walls of the container body are all deformed walls that become concave when subjected to the extrusion force from the outside to the inside.

4. The urine exfoliated cell collection device according to any one of claims 1 to 3, characterized in that: The connector includes a large diameter section and a small diameter section. The inner diameter of the large diameter section is larger than the inner diameter of the small diameter section. An internal step structure is formed at the junction of the inner wall surfaces of the large diameter section and the small diameter section. The connecting pipe section extends into the large diameter section and is fixedly connected to the large diameter section. The cell collection filter is pressed between the axial end face of the connecting pipe section and the step surface of the internal step structure.

5. The urine exfoliated cell collection device according to claim 4, characterized in that: The connecting pipe section is threadedly connected to the large diameter section of the connecting head.

6. The urine exfoliated cell collection device according to any one of claims 1 to 3, characterized in that: The urine exfoliated cell collection device also includes a waste liquid bottle, the upper end of which is provided with a waste liquid inlet for the filtered urine to enter, and a support structure for supporting the connector or the specimen container is provided at the waste liquid inlet.

7. The urine exfoliated cell collection device according to claim 6, characterized in that: The connector includes a large diameter section and a small diameter section. The outer diameter of the large diameter section is larger than the outer diameter of the small diameter section. An outer step structure is formed at the junction of the outer wall surfaces of the large diameter section and the small diameter section. The large diameter section is connected to the connecting pipe section. The inner diameter of the waste liquid inlet is larger than the outer diameter of the small diameter section and smaller than the outer diameter of the large diameter section. The upper end of the waste liquid bottle is used to support the step surface of the outer step structure after the small diameter section is inserted into the waste liquid inlet. The upper end of the waste liquid bottle constitutes a supporting structure.

8. The urine exfoliated cell collection device according to claim 6, characterized in that: The inner diameter of the waste liquid inlet is larger than the outer diameter of the large diameter section and smaller than the outer diameter of the container body. The upper end of the waste liquid bottle is used to support the side wall of the container body where the connecting pipe section is located after the connecting head passes through the waste liquid inlet. The upper end of the waste liquid bottle constitutes a supporting structure.

9. The urine exfoliated cell collection device according to claim 6, characterized in that: The connector includes a large diameter section and a small diameter section. The inner diameter of the large diameter section is larger than the inner diameter of the small diameter section. An internal step structure is formed at the junction of the inner wall surfaces of the large diameter section and the small diameter section. The large diameter section is connected to the connecting port. The waste liquid inlet is a tubular port, which extends into the large diameter section and is fixedly connected to the large diameter section. The cell collection filter is pressed between the axial end face of the tubular port and the step surface of the internal step structure.

10. The urine exfoliated cell collection device according to any one of claims 1 to 3, characterized in that: The urine exfoliated cell collection device also includes a bracket, which includes a base, a vertical frame fixed on the base, and a suspension fixed on the vertical frame. A support ring is provided on the suspension. The inner diameter of the support ring is larger than the outer diameter of the large diameter section and smaller than the outer diameter of the container body. The support ring is used to support the side wall of the container body where the connecting pipe section is located after the connecting head passes through.

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