Cell culture box

Abstract

The utility model provides a cell culture box, which belongs to the technical field of laboratory consumables, and is used for solving the problems that the ventilation requirement of cell culture needs to be met by replacing two bottle caps when a conventional cell culture bottle is used, and the operation is inconvenient and the harvesting efficiency is low in the harvesting process of adherent cells. According to the cell culture dish, the cell culture dish is designed into the cell culture box, the bottom plate of the box body is replaced by the glass slide, and the glass slide is fixed by additionally arranging the clamping assemblies on the side walls of the box body, so that the glass slide is convenient to mount and take; the ventilation requirement in the cell culture process is met by forming the ventilation hole in the side wall to be matched with the air filter membrane, and the operation hole is formed in the top wall to be matched with the heat-sealed transparent membrane, so that an experimenter can conveniently adjust the sealing state of the operation hole through the transparent membrane to perform experiment operation.

Description

Technical Field

[0001] The utility model belongs to the technical field of laboratory consumables, in particular to a cell culture box. Background Art

[0002] Cell culture dishes are essential equipment for drug and implantable medical device testing. Their high sealing and safety make them widely favored by researchers in the pharmaceutical and medical device industries. Cell culture dishes can be categorized by material: glass or plastic. The bottom of a cell culture dish can be shaped like a circle, square, rectangle, or triangle. Cell culture dishes come in a variety of sizes, ranging from 500ml to 3L.

[0003] Currently, most cell culture dishes on the market are cell culture flasks, which consist of a flask body and a removable cap. Traditional cell culture flasks utilize two caps that can be switched to achieve ventilation requirements for cell culture. While some cells and tissues can grow in suspension, a significant portion of mammalian cells adhere to the surface of the cell wall. Harvesting requires inserting a scraper into the flask through the flask opening, making operation inconvenient and resulting in low cell harvest rates. Utility Model Content

[0004] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a cell culture box to solve the problems that the existing cell culture bottles need to replace two bottle caps when in use to meet the ventilation requirements of cell culture, and the problem that the adherent cells are inconvenient to operate and have low harvesting efficiency during the harvesting process.

[0005] To achieve the above-mentioned and other related purposes, the present invention provides a cell culture box, comprising a box body, wherein the bottom plate of the box body is a glass slide, and a clamping assembly is installed at the bottom end of the box body side wall, and the clamping assembly is used to clamp and fix the glass slide.

[0006] Optionally, the clamping assembly includes a slide hook and a slide clamping plate;

[0007] The slide hook is fixedly connected to the bottom of the side wall of the box body;

[0008] The glass slide clamping plate is fixedly connected to the side wall away from the glass slide hook, and the glass slide clamping plate is a vertically arranged triangular plate; the vertical side of the glass slide clamping plate is fixedly connected to the side wall of the box body; the bottom edge of the glass slide clamping plate is away from the end of the side wall of the box body away from the slide, and forms a V-shaped gap with the slide; a clamping piece is fixedly connected to the long side of the glass slide clamping plate, and the clamping piece is a V-shaped clamping piece, including two support columns; a trigger is provided on the support column fixedly connected to the glass slide clamping plate, and the other support column is arranged vertically and fixedly connected to a hook at the bottom end.

[0009] Optionally, the slide includes a slide frosting area and a slide culture area; the slide culture area is adapted to the shape of the box body and cooperates with the slide hook on the side wall of the box body; the end of the slide frosting area away from the slide culture area cooperates with the hook.

[0010] Optionally, a scratch is provided on the center axis of the glass slide along the length direction, so as to facilitate separation of the glass slide along the center axis along the length direction.

[0011] Optionally, a positioning block is provided at the top end of the side wall of the box body, and the positioning blocks are multiple and symmetrically arranged.

[0012] Optionally, a ventilation hole is further provided on the side wall of the box body, and a breathable filter membrane is fixedly connected to the outside of the ventilation hole.

[0013] Optionally, an operation hole is opened on the top wall of the box body, and a transparent film is fixedly connected to the outside of the operation hole, and the closed state of the operation hole is adjusted by the transparent film.

[0014] Optionally, a support plate is further installed on the top wall of the box body, and there are two support plates that are symmetrically arranged.

[0015] As described above, the cell culture box of the present invention has at least the following beneficial effects:

[0016] The utility model designs the cell culture dish into a cell culture box, replaces the bottom plate of the box body with a glass slide, and the glass slide is fixed to the bottom of the box body by clamping with a clamping assembly installed on the side wall of the box body. By designing the clamping assembly into a glass slide hook and a glass slide clamping plate, a clamping piece is added to the glass slide clamping plate, and the glass slide is fixed and removed by the trigger and hook on the clamping piece, thereby avoiding the situation in which a scraper needs to be inserted into the culture bottle from the bottle mouth during the harvesting process of the traditional cell culture bottle, which is inconvenient to operate and has a low cell harvest rate.

[0017] The utility model realizes the ventilation requirement in the cell culture process by opening ventilation holes in the side wall of the box body and cooperating with the air filter membrane, and opens operation holes on the top wall of the box body and cooperating with the heat-sealed transparent film, so that the experimenter can adjust the sealing state of the operation hole through the transparent film to carry out the experimental operation.

[0018] The utility model also adds symmetrically arranged positioning blocks on the top of the box body side wall, which facilitates the stacking and storage of multiple cell culture boxes. In conjunction with the support plate installed on the top wall of the box body, gaps can be left between the stacked cell culture boxes, avoiding the difficulty of separating multiple cell culture boxes due to close fit when they are stacked flat. At the same time, it also facilitates air circulation, ensuring that the cell culture process is always at a suitable temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 It is a schematic diagram of the overall structure of the utility model;

[0021] Figure 3 This is a schematic diagram of multiple cell culture boxes stacked in the present invention.

[0022] Component number description

[0023] Box body-1, slide-2, slide hook-3, slide clamping plate-4, clamping part-401, trigger-402, air vent-5, operation hole-6, positioning block-7, support plate-8. DETAILED DESCRIPTION

[0024] The following describes the implementation of the present invention through specific embodiments. People familiar with this technology can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0025] See also Figures 1 to 3 . It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of this utility model. Therefore, they have no substantive technical significance. Any modification of the structure, change in the proportional relationship or adjustment of the size should still fall within the scope of the technical content disclosed by this utility model without affecting the efficacy and purpose that can be achieved by this utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of this utility model. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of this utility model without substantially changing the technical content.

[0026] The following embodiments are for illustration only and can be combined with each other, and are not limited to the contents presented in the following single embodiments.

[0027] See also Figure 1 and Figure 2The utility model provides a cell culture box with a slide 2, comprising a box body 1, the bottom plate of the box body 1 is a slide 2, and the bottom end of the side wall of the box body 1 is equipped with a clamping assembly, which is used to clamp and fix the slide 2, and the clamping assembly includes a slide hook 3 and a slide clamping plate 4; the slide hook 3 is fixedly connected to the bottom of the side wall of the box body 1; the slide clamping plate 4 is fixedly connected to the side wall away from the slide hook 3, and the slide clamping plate 4 is a vertically arranged triangular plate; the vertical side of the slide clamping plate 4 is fixedly connected to the side wall of the box body 1; the bottom edge of the slide clamping plate 4 is away from the end of the side wall of the box body 1 away from the slide 2, and forms a V-shaped gap with the slide 2 (for easy installation and removal of the slide 2); the long side of the slide clamping plate 4 is fixedly connected with a clamping piece 401, and the clamping piece 401 is a V-shaped clamping piece 401, including two support columns; fixed with the slide clamping plate 4 The hopper 402 is fixed to the bottom of the hopper 4 by means of a trigger 402 and a hook 403 on the other end of the hopper 404. ... scraper 402 and a hook 403 on the other end of the hopper 404. The hopper 402 is fixed to the bottom of the hopper 4 by means of a clip 402 and a hook 403 on the other end of the hopper 404. The hopper 402 is fixed to the bottom of the hopper 4 by means of a clip 402 and a hook 403 on the other end of the hopper 404. The hopper 402 is fixed to the bottom of the hopper 4 by means of a clip 402 and a hook 403 on the other end of the hopper 404. The hopper 402 is fixed to the bottom of the hopper 4 by means of a clip

[0028] In this embodiment, a scratch is provided on the center axis of the glass slide 2 along the length direction, so that the glass slide 2 can be separated along the center axis along the length direction to form two independent glass slides, thereby increasing the number of glass slides.

[0029] In this embodiment, please refer to Figure 1 and Figure 3 A positioning block 7 is provided at the top of the side wall of the box body 1. The positioning blocks 7 are multiple and symmetrically arranged. The installation of the positioning blocks 7 facilitates the stacking and storage of multiple cell culture boxes. A support plate 8 is also installed on the top wall of the box body 1. The support plates 8 are two and symmetrically arranged. The installation of the support plates 8 can leave gaps between the stacked cell culture boxes, avoiding the difficulty of separating multiple cell culture boxes due to close fit when they are stacked flat. At the same time, it also facilitates air circulation, ensuring that the cell culture process is always at a suitable temperature.

[0030] In this embodiment, please refer to Figure 1The side wall of the box body 1 is also provided with an air hole 5, and a breathable filter membrane is fixedly connected to the outside of the air hole 5. The air hole cooperates with the air filter membrane to achieve the ventilation requirements during the cell culture process. The top wall of the box body 1 is provided with an operation hole 6, and a transparent film is fixedly connected to the outside of the operation hole 6. The closing state of the operation hole 6 is adjusted by the transparent film. The operation hole 6 is combined with a heat-sealed transparent film, which makes it convenient for the experimenter to adjust the closing state of the operation hole 6 through the transparent film to perform experimental operations.

[0031] In summary, the present invention overcomes various shortcomings in the prior art.

[0032] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed in the present invention are intended to be covered by the claims of the present invention.

Claims

1. A cell culture box, comprising a box body, characterized in that: The bottom plate of the box body is a glass slide, and the bottom end of the side wall of the box body is additionally provided with a clamping assembly, which is used to clamp and fix the glass slide; The clamping assembly includes a slide hook and a slide clamping plate; The slide hook is fixedly connected to the bottom of the side wall of the box body; The glass slide clamping plate is fixedly connected to the side wall away from the glass slide hook, and the glass slide clamping plate is a vertically arranged triangular plate; the vertical side of the glass slide clamping plate is fixedly connected to the side wall of the box body; the bottom edge of the glass slide clamping plate is away from the end of the side wall of the box body away from the slide, and forms a V-shaped gap with the slide; a clamping piece is fixedly connected to the long side of the glass slide clamping plate, and the clamping piece is a V-shaped clamping piece, including two support columns; a trigger is provided on the support column fixedly connected to the glass slide clamping plate, and the other support column is arranged vertically and fixedly connected to a hook at the bottom end.

2. The cell culture cassette according to claim 1, wherein: The slide comprises a glass frosting area and a glass slide culture area; the glass slide culture area is adapted to the shape of the box body and cooperates with the glass slide hook on the side wall of the box body; the end of the glass slide frosting area away from the glass slide culture area cooperates with the hook.

3. The cell culture cassette according to claim 1, wherein: The glass slide is provided with scratches on the central axis along the length direction, so as to facilitate separation of the glass slide along the central axis along the length direction.

4. The cell culture cassette according to claim 1, wherein: A positioning block is provided at the top end of the side wall of the box body, and the positioning blocks are multiple and symmetrically arranged.

5. The cell culture cassette according to claim 1, wherein: A ventilation hole is also provided on the side wall of the box body, and a ventilation filter membrane is fixedly connected to the outside of the ventilation hole.

6. The cell culture cassette according to claim 1, wherein: An operating hole is opened on the top wall of the box body, and a transparent film is fixedly connected to the outside of the operating hole, and the closed state of the operating hole is adjusted by the transparent film.

7. The cell culture cassette according to claim 1, wherein: A support plate is further installed on the top wall of the box body, and there are two support plates that are symmetrically arranged.

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