Filtering device for sterilizing culture medium

Abstract

The utility model discloses a filtering device for sterilizing a culture medium, and belongs to the field of biological equipment. The filtering device comprises a filtering cylinder body, a filter element, a base bracket, a feeding hole, a discharging hole and a transparent bottle, the filter element is arranged in the filter cylinder body and divides the internal space of the filter cylinder body into a filter element inner side area and a filter element outer side area; the feeding hole is formed in the bottom of the filter cylinder body and is communicated with the outer side area of the filter element in the filter cylinder body; the discharge hole is formed in the bottom of the filter cylinder body and is communicated with the inner side area of the filter element in the filter cylinder body; the transparent bottle is arranged outside the filter cylinder and is communicated with the outer side area of the filter element in the filter cylinder through a pipeline, and a valve is arranged on the pipeline. According to the filtering device, the color of the culture medium can be observed conveniently through the external transparent bottle, sampling can be facilitated, and abnormal conditions of the culture medium can be found in time.

Description

Technical Field

[0001] The utility model belongs to the field of biological equipment, and in particular relates to a filtering device for sterilizing a culture medium. Background Art

[0002] Culture media are nutrient substrates composed of a combination of different nutrients that support the growth and reproduction of microorganisms, plants, and animal tissues. They are widely used in the field of biofermentation. In biofermentation production, to obtain specific metabolites or a large number of bacterial cells, only the producer bacteria are typically allowed to grow and reproduce, while other microorganisms are not allowed to coexist. However, fermentation systems are typically nutrient-rich and susceptible to contamination by other bacteria. If contamination occurs during the fermentation process, these bacteria compete with the producer bacteria for nutrients and may secrete substances that inhibit the growth of the producer bacteria, resulting in reduced productivity. Furthermore, the metabolites of these bacteria increase the variety of products, making their isolation difficult, and can severely alter the properties of the culture medium, inhibiting the synthesis and growth of the target product, or even causing product decomposition. To avoid these issues, the entire fermentation process must be carried out under sterile conditions, which requires sterilization. Sterilization can be performed using physical or chemical methods to remove all living organisms from materials and equipment. Heat-sensitive materials or culture media, particularly animal culture media, cannot be sterilized using steam due to the presence of heat-labile proteins. In the bio-fermentation industry, filtration sterilization is commonly used to treat heat-sensitive materials. Culture media can be filtered at room temperature to remove solid particles suspended in liquids or gases. Filtration sterilization is achieved when the removed solid particles are smaller than the microbial cells. Current filtration devices for culture media sterilization utilize stainless steel filter elements, filter housings, and transmission lines, making it difficult to observe the culture media and sample them, making it difficult to detect abnormalities in the culture media in a timely manner. Utility Model Content

[0003] The purpose of the utility model is to provide a filtering device for sterilizing a culture medium, which can facilitate observation of the color of the culture medium through an external transparent bottle, facilitate sampling, and timely discover abnormal conditions of the culture medium.

[0004] The technical solution adopted by the present invention to achieve the above-mentioned purpose is:

[0005] A filtering device for sterilizing a culture medium, comprising a filter cylinder, a filter element, a base support, a feed port, a discharge port, and a transparent bottle;

[0006] The filter cylinder is fixed on the base bracket;

[0007] The filter element is arranged in the filter cylinder, dividing the internal space of the filter cylinder into the inner area of ​​the filter element and the outer area of ​​the filter element;

[0008] The feed port is arranged at the bottom of the filter cylinder and is connected to the outer area of ​​the filter element in the filter cylinder;

[0009] The discharge port is arranged at the bottom of the filter cylinder and is connected to the inner area of ​​the filter element in the filter cylinder;

[0010] The transparent bottle is arranged outside the filter cylinder and is connected to the outer area of ​​the filter element in the filter cylinder through a pipeline, and a valve is arranged on the pipeline.

[0011] Furthermore, a pressure sensor is provided on the top of the filter cylinder, and the pressure sensor is used to monitor the pressure change inside the filter cylinder.

[0012] Furthermore, the transparent bottle is a colorless and transparent glass bottle.

[0013] Furthermore, the bottle mouth of the transparent bottle is equipped with a detachable screw-on bottle cap.

[0014] Furthermore, the screw-on bottle cap is made of polypropylene.

[0015] Furthermore, a pipe connected to the filter cylinder is provided on the bottle cap of the transparent bottle.

[0016] Furthermore, the valve on the pipeline connecting the transparent bottle and the filter cylinder is a three-way manual valve.

[0017] Furthermore, a respirator is connected to the bottle cap of the transparent bottle through a pipe, and the respirator is communicated with the inside of the transparent bottle through the pipe.

[0018] Furthermore, at least three outer legs are connected to the bottom of the base bracket, and the outer legs play a supporting role.

[0019] Furthermore, the lower end of the outer leg is hollow and tubular, and a slidable inner leg is provided inside the outer side of the lower end of the outer leg; an adjusting bolt is provided on the outer side of the lower end of the outer leg, and the adjusting bolt is tightened to prohibit the inner leg from sliding.

[0020] The filter device of the utility model has the following beneficial effects:

[0021] 1. Real-time monitoring and timely maintenance: By observing the color of the culture medium in the transparent bottle, the risk of bioreactor contamination due to culture medium contamination and untimely filter element replacement in the filter housing can be effectively reduced. The transparent bottle design allows operators to promptly detect abnormalities in the culture medium, allowing them to take appropriate maintenance measures to ensure normal operation of the system.

[0022] 2. Convenient Sampling and Microscopic Inspection: The transparent bottle not only facilitates observation of the culture medium's color but also allows for sampling. Culture medium samples removed from the transparent bottle can be examined under a microscope to further determine the culture medium's microscopic state. This feature facilitates culture medium quality control and microbiological testing.

[0023] 3. Flexible Height Adjustment: To adjust the height of the filter unit, simply loosen the adjustment bolts to extend the inner legs until the desired height is reached, then tighten the adjustment bolts. The leg design allows the filter cylinder to be flexibly adjusted to suit different operating environments, enhancing the unit's usability.

[0024] The filtration device realizes real-time monitoring of the culture medium status and convenient sampling through the setting of the transparent bottle, reducing the risk of contamination. At the same time, the adjustment function of the legs improves the applicability and ease of operation of the device, which has significant practical value. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Schematic diagram of the structure of a filtration device for sterilizing a culture medium according to an embodiment.

[0026] Description of reference numerals:

[0027] 1: Filter cylinder;

[0028] 2: filter element;

[0029] 3: Base bracket;

[0030] 4: Feed inlet;

[0031] 5: discharge port;

[0032] 6: Glass bottle;

[0033] 7: Respirator;

[0034] 8: pressure sensor;

[0035] 9: Three-way hand valve;

[0036] 10: Outer legs;

[0037] 11: Adjusting bolt;

[0038] 12: Inner leg. DETAILED DESCRIPTION

[0039] In order to make the various technical features and advantages or technical effects of the above technical solutions of the present invention more obvious and easy to understand, they are described in detail below with reference to the accompanying drawings.

[0040] The present invention specifically discloses a filtering device for sterilizing a culture medium, such as Figure 1 As shown, it includes a filter cylinder 1, a filter element 2, a base bracket 3, a feed port 4, a discharge port 5 and a glass bottle 6.

[0041] The filter cylinder 1 and the filter element 2 serve as the main parts of the filtration device, wherein a pressure sensor 8 is provided at the top of the filter cylinder 1 for monitoring the pressure changes inside the filter cylinder 1 in order to control the filtration process. The filter element 2 is arranged in the filter cylinder 1, dividing the internal space of the filter cylinder 1 into an inner area of ​​the filter element and an outer area of ​​the filter element. The filter element 2 can adopt a conventional design in which a filter membrane is wrapped on a cylindrical frame cover. The feed port 4 is arranged at the bottom of the filter cylinder 1 and is connected to the outer area of ​​the filter element in the filter cylinder 1. The discharge port 5 is arranged at the bottom of the filter cylinder 1 and is connected to the inner area of ​​the filter element in the filter cylinder 1.

[0042] The base bracket 3 serves as the foundation of the filter device, securing the filter cartridge 1. At least three outer legs 10 are connected to the bottom of the base bracket 3, providing support. The lower ends of the outer legs 10 are hollow tubular, housing a slidable inner leg 12. Adjustment bolts 11 are located on the outer sides of the lower ends of the outer legs 10. Tightening these bolts prevents the inner legs 12 from sliding.

[0043] The glass bottle 6, as the external portion of the filtration device, is mounted outside the filter cartridge 1 and connected to the outer area of ​​the filter element within the filter cartridge 1 via a pipe with a valve installed on the pipe. The mouth of the glass bottle 6 is equipped with a removable screw-on cap made of polypropylene. The cap of the glass bottle 6 is provided with a pipe connected to the filter cartridge 1, and the valve on the pipe is a three-way manual valve 9. The cap of the glass bottle 6 is connected to a respirator 7 via a peripheral pipe. This respirator 7 can be any commercially available respirator.

[0044] This filter device is mainly used for sterilizing culture medium. Its working principle is as follows:

[0045] The culture medium enters the filter cylinder 1 through the feed port 4 and then enters the outer area of ​​the filter element. Since the filter element 2 divides the internal space of the filter cylinder 1 into the inner area and the outer area of ​​the filter element, the culture medium must pass through the filter element 2 before entering the inner area of ​​the filter element. After being sterilized and filtered, it is discharged from the discharge port 5 to complete the filtration of the culture medium.

[0046] When it is necessary to observe the filtration condition of the culture medium, the operator can open the three-way hand valve 9 provided on the connecting pipe between the glass bottle 6 and the filter cylinder 1. At this time, part of the culture medium is discharged from the outer area of ​​the filter element through the pipe into the glass bottle 6. The excess air in the glass bottle 6 is discharged to the outside of the device through the respirator 7 connected to the bottle cap. When the culture medium in the glass bottle 6 reaches the volume required for observation, the three-way hand valve 9 is closed. At this time, the operator can observe the state of the culture medium through the glass bottle 6 to determine its color and whether there are any abnormalities. In addition, the sterilizing filter needs to be sterilized before use in the filtration process. Therefore, in addition to the above-mentioned uses, the three-way hand valve 9 can also be used to clear steam during online sterilization.

[0047] Glass bottle 6 not only facilitates observation but also can be used for culture medium sampling. To sample, first open three-way manual valve 9 to allow the culture medium to drain from filter cartridge 1 into glass bottle 6. After closing three-way manual valve 9, the operator can unscrew the screw-on cap on glass bottle 6 and remove the culture medium. After replacing the new glass bottle 6, tighten the sealing cap again. The culture medium in this glass bottle 6 can be used for further sample testing.

[0048] Although the present invention has been disclosed as above in the form of embodiments, they are not intended to limit the present invention. Appropriate modifications or equivalent substitutions of the technical solutions of the present invention by ordinary technicians in this field should be included in the protection scope of the present invention. The protection scope of the present invention shall be based on that defined in the claims.

Claims

1. A filtration device for sterilizing a culture medium, characterized in that: Including filter cylinder, filter element, base bracket, feed port, discharge port and transparent bottle; The filter cylinder is fixed on the base bracket; The filter element is arranged in the filter cylinder, dividing the internal space of the filter cylinder into the inner area of ​​the filter element and the outer area of ​​the filter element; The feed port is arranged at the bottom of the filter cylinder and is connected to the outer area of ​​the filter element in the filter cylinder; The discharge port is arranged at the bottom of the filter cylinder and is connected to the inner area of ​​the filter element in the filter cylinder; The transparent bottle is arranged outside the filter cylinder and is connected to the outer area of ​​the filter element in the filter cylinder through a pipeline, and a valve is arranged on the pipeline.

2. The filtration device for sterilizing a culture medium according to claim 1, wherein: A pressure sensor is provided on the top of the filter cylinder, which is used to monitor the pressure changes inside the filter cylinder.

3. The filtration device for sterilizing a culture medium according to claim 1, wherein: Transparent bottles should be made of colorless and transparent glass bottles.

4. The filtration device for sterilizing a culture medium according to claim 1, wherein: The mouth of the transparent bottle is equipped with a detachable screw-on cap.

5. The filtration device for sterilizing a culture medium according to claim 4, wherein: The screw-on cap is made of polypropylene.

6. The filtration device for sterilizing a culture medium according to claim 1, wherein: A pipe connected with the filter cylinder is arranged on the bottle cap of the transparent bottle.

7. The filtration device for sterilizing a culture medium according to claim 6, wherein: The valve on the pipeline connecting the transparent bottle and the filter cylinder is a three-way hand valve.

8. The filtration device for sterilizing a culture medium according to claim 1, wherein: A respirator is connected to the bottle cap of the transparent bottle through a pipeline, and the respirator is communicated with the inside of the transparent bottle through the pipeline.

9. The filtration device for sterilizing a culture medium according to claim 1, wherein: At least three outer legs are connected to the bottom of the base bracket, and the outer legs play a supporting role.

10. The filtration device for sterilizing a culture medium according to claim 9, wherein: The lower end of the outer leg is in the shape of a hollow tube, in which a slidable inner leg is arranged; an adjusting bolt is arranged on the outer side of the lower end of the outer leg, and the adjusting bolt is tightened to prohibit the inner leg from sliding.

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