Bacterial colony counting culture dish

Abstract

The bacterial colony counting culture dish comprises a dish body and a top cover, an assembling dish part is integrally formed above the dish body, the dish body and the assembling dish part are matched to form a clamping limiting table, and the top cover is buckled above the dish body. The bacterial colony separation assembly is assembled in the dish body, the bacterial colony separation assembly is completely stored between the dish body and the top cover, the top cover is composed of a cover body and a clamping ring, the clamping ring is arranged on the outer side of the cover body in a sleeving mode, the clamping ring and the cover body are integrally formed, and the clamping ring and the clamping limiting table are arranged in a matched mode. The bacterial colony separation assembly is used for separating the inner bottom surface of the dish body into two parts. According to the bacterial colony counting culture dish disclosed by the utility model, a blank area with uniform width and standard linear state can be formed in the culture dish by arranging the bacterial colony separating assembly, so that the accuracy of a cell scratch test is ensured, an additional scratch tool is not needed, the process of the cell scratch test is simplified, the operation repeatability is strong, and the working efficiency is high. And the adverse effect of human factors on the cell scratch test is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of colony counting culture dishes, in particular to a colony counting culture dish. Background Art

[0002] A culture dish is a type of laboratory vessel used for microbial or cell culture. It usually consists of a flat, disc-shaped bottom plate and a top cover. It is generally made of glass or plastic and is suitable for laboratory inoculation, streaking, and bacterial isolation operations.

[0003] The cell scratch assay is an experimental technique that studies cell migration, repair, and intercellular interactions by scratching a cell monolayer and capturing images periodically through time-lapse microscopy. Cell migration, the movement of cells under the guidance of signals, is a universal form of movement in living cells. Cell migration is one of the fundamental functions of cells in normal physiological processes and is involved in processes such as embryonic development, angiogenesis, wound healing, immune response, inflammatory response, atherosclerosis, and cancer metastasis.

[0004] When performing a cell scratch assay, a sterile pipette tip or toothpick is typically used to draw a line on the culture dish. The assay is then performed by observing the migration of cells from the two sides of the line toward the blank area in the middle. However, in practice, this method not only fails to control the width of the blank area, but the drawn line is also easily affected by human factors and is difficult to be straight. Both the width of the blank area and the straightness of the line can have a negative impact on the cell scratch assay.

[0005] Therefore, in response to the above technical problems, it is necessary to provide a colony counting culture dish.

[0006] The information disclosed in this background technology section is only intended to increase the understanding of the overall background of the present invention, and should not be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. Utility Model Content

[0007] The utility model aims to provide a colony counting culture dish, which can produce a blank area with uniform width and standard straight line state, thereby ensuring the accuracy of a cell scratch test.

[0008] In order to achieve the above-mentioned purpose, a specific embodiment of the present invention provides a colony counting culture dish, including: a dish body and a top cover, wherein an assembly dish piece is integrally formed above the dish body, the dish body and the assembly dish piece cooperate to form a locking limit platform, and the top cover is buckled on the top of the dish body.

[0009] The colony separation component is assembled in the dish body, and the colony separation component is completely accommodated between the dish body and the top cover. The colony separation component is used to separate the inner bottom surface of the dish body into two parts.

[0010] In one or more embodiments of the present invention, the top cover is composed of a cover body and a locking ring. The locking ring is fitted onto the outside of the cover body and is integrally formed with the cover body. The locking ring cooperates with the locking stop. The top cover is fastened to the dish body, ensuring the dish's airtightness for cell culture.

[0011] In one or more embodiments of the present invention, the colony separation component is a partition structure, which is assembled in the dish body, and the upper surface of the partition structure is at the same level as the upper surface of the assembled dish.

[0012] In one or more embodiments of the present invention, the partition structure includes a first partition having arc-shaped sides, the sides of the first partition being attached to the inner wall of the dish body, and the first partition being inserted into the dish body. The first partition divides the dish body into two parts, thereby facilitating the formation of a uniformly wide, straight, and standard blank area at the bottom of the dish body by removing and installing the first partition.

[0013] In one or more embodiments of the present invention, the partition structure includes a second partition, which is composed of a partition body and a pair of positioning members, and the pair of positioning members are integrally formed on both sides of the partition body.

[0014] In one or more embodiments of the present invention, a pair of the positioning members are arranged in an arc shape on a side away from the partition body, the positioning members are attached to the inner wall of the dish body, and the second partition is inserted into the dish body.

[0015] In one or more embodiments of the present invention, the colony separation component is a belt structure, and the belt structure is attached to the inner wall of the dish body by electrostatic or other non-adhesive means.

[0016] In one or more embodiments of the present invention, the belt structure includes a scratch belt, which is attached to the inner wall of the dish body. The scratch belt consists of a belt body and a tear belt, and the tear belt is integrally formed at one end of the belt body.

[0017] In one or more embodiments of the present invention, the length of the strip is greater than the sum of the diameter of the dish and the height of the dish and the assembled dish components, and the tear strip is located outside the dish after the scratch strip is attached to the inner wall of the dish, so that the strip can be easily removed by pulling the tear strip.

[0018] Compared with the prior art, the colony counting culture dish disclosed in the present invention can form a blank area with uniform width and standard straight line state in the culture dish by setting a colony separation component, thereby ensuring the accuracy of the cell scratch test;

[0019] At the same time, no additional scratching tools are required, which simplifies the cell scratch test process, increases the repeatability of the operation, and reduces the adverse effects of human factors on the cell scratch test. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are only some of the embodiments described in the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without inventive efforts.

[0021] Figure 1 This is a schematic structural diagram of a colony counting culture dish in the first embodiment of the present invention;

[0022] Figure 2 This is a schematic diagram of the plug-in assembly state of the colony counting culture dish in the first embodiment of the present utility model;

[0023] Figure 3 This is a top view of the plug-in assembly state of the colony counting culture dish in the first embodiment of the present utility model;

[0024] Figure 4 This is a cross-sectional view of a top cover in one embodiment of the present utility model;

[0025] Figure 5 This is a schematic structural diagram of a colony counting culture dish in the second embodiment of the present invention;

[0026] Figure 6 This is a schematic diagram of the plug-in assembly state of the colony counting culture dish in the second embodiment of the present utility model;

[0027] Figure 7 This is a top view of the plug-in assembly state of the colony counting culture dish in the second embodiment of the present invention;

[0028] Figure 8 This is a schematic structural diagram of a colony counting culture dish in the third embodiment of the present invention;

[0029] Figure 9 This is a schematic diagram of the plug-in assembly state of the colony counting culture dish in the third embodiment of the present utility model;

[0030] Figure 10 Schematic diagram of the fastening state of the colony counting culture dish in the third embodiment of the present invention.

[0031] Description of main reference numerals:

[0032] 1-dish body, 101-dish assembly part, 2-top cover, 201-cover body, 202-clamping ring, 3-first partition, 4-second partition, 401-partition plate body, 402-positioning piece, 5-scratch tape, 501-belt body, 502-tear tape. DETAILED DESCRIPTION

[0033] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the following will provide a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only a portion of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

[0034] like Figures 1 to 10 As shown, a specific embodiment of the present invention provides a colony counting culture dish, comprising: a dish body 1 and a top cover 2. The dish body 1 and the top cover 2 are buckled together to form a closed culture dish, which provides a culture foundation for cell colony culture.

[0035] like Figures 1 to 10 As shown, an assembly dish piece 101 is integrally formed on the upper portion of the dish body 1, and the dish body 1 and the assembly dish piece 101 cooperate to form a clamping limit platform, and the top cover 2 is buckled on the upper portion of the dish body 1. The clamping ring 202 is clamped and limited by the clamping limit platform.

[0036] like Figures 1 to 10 As shown, the colony separation assembly is assembled in the dish body 1 , and the colony separation assembly is completely accommodated between the dish body 1 and the top cover 2 . The colony separation assembly is used to separate the inner bottom surface of the dish body 1 into two parts.

[0037] like Figure 4 As shown, the top cover 2 consists of a cover body 201 and a locking ring 202. The locking ring 202 is mounted on the outside of the cover body 201 and is integrally formed with the cover body 201. The locking ring 202 cooperates with the locking stopper. The top cover 2 is fastened to the dish body 1, ensuring the airtightness of the dish body 1 for cell culture.

[0038] Specifically, the colony separation assembly is a partition structure, which is assembled in the dish body 1. The upper surface of the partition structure is at the same level as the upper surface of the assembly dish 101. The partition structure divides the dish body 1 into two parts, so that a blank area with uniform width and standard straight line shape can be formed in the dish body 1 by disassembling and assembling the partition structure.

[0039] Example 1:

[0040] like Figures 1 to 3 As shown, the colony counting culture dish in the first embodiment of the present invention has a partition structure including a first partition 3, both sides of which are arranged in an arc shape. Both sides of the first partition 3 are attached to the inner wall of the dish body 1. By arranging the two sides of the first partition 3 in an arc shape, the airtightness of the first partition 3 is ensured when the dish body 1 is separated by the first partition 3.

[0041] The bottom of the dish body 1 is provided with a snap-fitting groove, through which the first partition 3 is inserted into the dish body 1. The first partition 3 divides the dish body 1 into two parts, so that a blank area of ​​uniform width and standard straight line shape can be formed at the bottom of the dish body 1 by disassembling and assembling the first partition 3.

[0042] like Figures 1 to 2 As shown, during use, the first partition 3 is inserted into the engaging groove, and the dish body 1 is divided into two parts by the first partition 3. Subsequently, cells are seeded in the dish body 1, and after the cells are adherently cultured to the desired density for the experiment, the first partition 3 is removed. A cell-free area of ​​uniform width and ratio appears at the location of the first partition 3, which is the cell scratch.

[0043] Example 2:

[0044] like Figures 5 to 7 As shown, the partition structure includes a second partition 4, which is composed of a partition body 401 and a pair of positioning members 402. The pair of positioning members 402 are integrally formed on both sides of the partition body 401. The partition body 401 and the positioning members 402 cooperate to separate the dish body 1 into two parts.

[0045] Specifically, a pair of positioning members 402 are arranged in an arc shape on one side away from the partition plate body 401, and the positioning members 402 are attached to the inner wall of the dish body 1, and the second partition plate 4 is inserted into the dish body 1. The mutual contact between the pair of positioning members 402 and the inner wall of the dish body 1 ensures the insertion stability of the second partition plate 4.

[0046] like Figures 5 to 7 As shown, during use, the second separator 4 is inserted into the engaging groove. During the insertion process, the pair of positioning members 402 contact the inner wall of the dish 1, ensuring the insertion stability of the separator body 401. Subsequently, cells are seeded into the dish 1 and cultured adherently to the wall to the desired density. The second separator 4 is then removed. A straight, uniformly wide, cell-free area, representing the cell scratch, appears at the location of the second separator 4.

[0047] Example 3:

[0048] Specifically, the colony separation assembly is a belt structure that is attached to the inner wall of the dish 1 by electrostatic or other non-adhesive means. The belt structure divides the dish 1 into two parts, thereby facilitating the formation of a uniform and straight cell scratch on the bottom of the dish 1 by attaching or tearing the belt 501.

[0049] like Figures 8 to 10 As shown, the strip structure includes a scoring strip 5, which is attached to the inner wall of the dish 1. The scoring strip 5 consists of a strip body 501 and a tear strip 502. The strip body 501 forms a cell scratch on the bottom surface of the dish 1. The tear strip 502 is integrally formed at one end of the strip body 501. The scoring strip 5 is removed by tearing the tear strip 502.

[0050] Specifically, the length of the tape 501 is greater than the sum of the diameter of the dish body 1 and the height of the dish body 1 and the assembly dish 101. After the scratch tape 5 is attached to the inner wall of the dish body 1, the tear tape 502 is located on the outer side of the dish body 1. The tape 501 can be easily removed by pulling the tear tape 502.

[0051] Preferably, the belt body 501 of different widths can be selected according to different requirements of the cell scratch test.

[0052] like Figures 8 to 10 As shown, during use, the strip 501 is attached to the inner wall of the dish 1 using electrostatic or non-adhesive means. Cells are then seeded into the dish 1 and allowed to adhere to the wall and grow to the desired density. The strip 501 can then be removed by pulling the tear strip 502 from the side of the dish 1. The strip 501 and the cells grown on it are discarded, leaving a straight, uniformly wide scratch where the strip 501 originally was.

[0053] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be encompassed within the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0054] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A colony counting culture dish, characterized in that: include: A dish body and a top cover, wherein an assembly dish piece is integrally formed on the top of the dish body, the dish body and the assembly dish piece cooperate to form a locking limit platform, and the top cover is buckled on the top of the dish body; A colony separation component is assembled in the dish body; The colony separation component is completely accommodated between the dish body and the top cover, and is used for separating the inner bottom surface of the dish body into two parts.

2. The colony counting culture dish according to claim 1, characterized in that The top cover is composed of a cover body and a clamping ring. The clamping ring is sleeved on the outside of the cover body, and the clamping ring and the cover body are integrally formed. The clamping ring is arranged in cooperation with the clamping limit platform.

3. The colony counting culture dish according to claim 1, characterized in that The colony separation component is a partition structure, which is assembled in the dish body. The upper surface of the partition structure and the upper surface of the assembled dish are at the same level.

4. The colony counting culture dish according to claim 3, characterized in that: The partition structure includes a first partition. Both sides of the first partition are arranged in an arc shape. Both sides of the first partition are attached to the inner wall of the dish body. The first partition is inserted into the dish body.

5. The colony counting culture dish according to claim 3, characterized in that: The partition structure includes a second partition, which is composed of a partition body and a pair of positioning members. The pair of positioning members are integrally formed on both sides of the partition body.

6. The colony counting culture dish according to claim 5, characterized in that: A pair of positioning members are arranged in an arc shape on one side away from the partition body. The positioning members are attached to the inner wall of the dish body, and the second partition is inserted into the dish body.

7. The colony counting culture dish according to claim 1, characterized in that: The colony separation component is a belt-type structure, and the belt-type structure is attached to the inner wall of the dish body by static electricity or other non-adhesive methods.

8. The colony counting culture dish according to claim 7, characterized in that: The belt structure includes a scratch belt, which is attached to the inner wall of the dish body. The scratch belt consists of a belt body and a tear belt, and the tear belt is integrally formed at one end of the belt body.

9. The colony counting culture dish according to claim 8, characterized in that: The length of the strip body is greater than the sum of the diameter of the dish body and the height of the dish body and the assembled dish parts. After the scratch strip is attached to the inner wall of the dish body, the tear strip is located on the outer side of the dish body.

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