Dendrobium protocorm stem cell proliferation culture system

Abstract

The utility model relates to the technical field of plant biology, in particular to a dendrobium protocorm stem cell proliferation culture system which comprises an incubator, a fixed tray, a movable tray, an adjusting device, a humidifier, a timing reciprocating device and a moving device. The humidifier is installed on the back wall of an inner cavity of the cultivation box through the timing reciprocating device, the multiple sets of fixed trays are evenly distributed on the lower middle portion in the cultivation box, the movable tray is installed on the upper portion of the cultivation box through the adjusting device, a first sliding block on a first screw rod moves up and down along with rotation of the first screw rod, and a second sliding block on a second screw rod moves up and down along with rotation of the second screw rod. Due to the fact that the first sliding block is fixedly connected with the movable tray, the movable tray ascends and descends along with the movable tray, the height of the movable tray is adjusted, and the height of a culture dish for dendrobium protocorm needing to be cultured and placed in the movable tray can be adjusted conveniently.

Description

Technical Field

[0001] The utility model relates to the field of plant biotechnology, in particular to a Dendrobium protocorm stem cell proliferation and culture system. Background Art

[0002] As we all know, Dendrobium is a tonic medicine. This product is the cultivated products of Orchidaceae plants Dendrobium nobile, Huoshan Dendrobium, Chrysotoxum or Tassel Dendrobium and their similar species of the same genus. Dendrobium is an important Chinese medicinal material, which contains a variety of biologically active substances and has high medicinal value.

[0003] In traditional Dendrobium proliferation and cultivation systems, especially when handling trays at higher positions, ladders or other auxiliary tools are required, which not only increases the complexity of the operation but also may bring safety hazards, and humidity control is usually difficult to achieve uniform distribution. Utility Model Content

[0004] (1) Technical problems solved

[0005] In view of the deficiencies in the prior art, the utility model provides a Dendrobium protocorm stem cell proliferation and culture system.

[0006] (2) Technical solution

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a system for the proliferation and cultivation of stem cells of Dendrobium protocorm, comprising an incubator, a fixed tray, a movable tray, an adjusting device, a humidifier, a timed reciprocating device, and a moving device, wherein the front end face of the incubator is provided with a door, the back wall of the inner cavity of the incubator is provided with the humidifier through the timed reciprocating device, a plurality of groups of the fixed trays are evenly distributed in the middle and lower parts of the incubator, the movable tray is provided on the upper part of the incubator through the adjusting device, the adjusting device comprises a first screw, a first slider, an active bevel gear, a driven bevel gear and a drive shaft, and a groove is provided on the upper side wall of one end of the incubator. The first screw is rotatably installed in the groove, and the first slider is threadedly installed on the first screw, and the side wall of the first slider is fixedly connected to the side wall of the movable tray. A cavity is opened in the side wall of the incubator close to the groove, and the bottom end of the first screw passes through the bottom wall of the groove and extends to the cavity where the driven bevel gear is sleeved. One end of the driven bevel gear is meshed and connected with the active bevel gear, and one end of the active bevel gear passes through the side wall of the incubator and is installed with the drive shaft. The fixed tray, the movable tray and the top wall of the incubator are all installed with fluorescent lamps, and the bottom wall of the incubator is installed with the moving device.

[0008] In order to facilitate the humidifier to evenly distribute moisture, the utility model has improvements in that the positioning reciprocating device includes a second screw, a second slider and a drive motor, a rectangular groove is opened on the back wall of the incubator, the second screw is rotatably installed in the rectangular groove, the second screw is threadedly installed with the second slider, the humidifier is installed on the side wall of the second slider, and the top end of the second screw passes through the top wall of the incubator and is installed with the drive motor.

[0009] In order to guide the second slider, the present invention has an improvement in that two groups of fixing rods are symmetrically installed in the rectangular groove with respect to the central axis of the second screw rod, and the two groups of fixing rods are both slidably connected to the second slider.

[0010] In order to guide the movement of the first slider, the utility model has an improvement in that a guide groove is provided on the side wall of the incubator away from the groove, a guide rod is fixedly installed in the guide groove, the guide block is slidably installed on the guide rod, and the side wall of the guide block is fixedly connected to the side wall of the movable tray away from the first slider.

[0011] In order to facilitate driving the driving shaft, the utility model is improved in that the end of the driving shaft is sleeved with a hand wheel.

[0012] In order to improve the anti-skid property of the hand-cranked wheel, the utility model is improved in that the outer wall of the hand-cranked wheel is provided with anti-skid grooves.

[0013] In order to ensure the stability and accuracy of the operation of the driving motor, the utility model is improved in that the driving motor is a servo motor.

[0014] In order to facilitate the movement and fixation of the incubator, the present invention has been improved in that the moving device includes universal wheels and a brake assembly. The four corners of the bottom end of the incubator are equipped with universal wheels, and the brake assembly is provided on the universal wheels, and the brake assembly is adapted to the universal wheels.

[0015] (3) Beneficial effects

[0016] Compared with the existing technology, the present invention provides a Dendrobium protocorm stem cell proliferation and culture system, which has the following beneficial effects:

[0017] The Dendrobium protocorm stem cell proliferation and culture system uses an adjustment device to adjust the height of the movable tray by rotating the drive shaft. By adjusting the height of the movable tray, it is easier for operators to take and place culture dishes in the tray, and there is no need to use external tools to adjust the tray to a higher position in the incubator. The operation is simple and quick, which can save a lot of time and energy and improve overall work efficiency.

[0018] The Dendrobium protocorm stem cell proliferation and culture system has a timed reciprocating device, a humidifier installed on a second slider, and a driving motor drives the second screw to rotate, so that the second slider performs reciprocating linear motion along the second screw inside the incubator. This ensures that the humidifier evenly distributes moisture throughout the incubator, avoiding localized excessively high or low humidity. The even distribution of the humidifier prevents certain areas from being overly humid, thereby avoiding disease problems caused by localized water accumulation and ensuring that the Dendrobium protocorms grow under consistent humidity conditions.

[0019] The Dendrobium protocorm stem cell proliferation and culture system uses a mobile device and the convenient mobility of universal wheels, allowing the operator to quickly adjust the position of the incubator, reducing the time spent on position adjustment and improving work efficiency. After using the brake assembly to lock the universal wheels, the incubator can be effectively prevented from being displaced due to external interference, protecting the cultures inside from damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the three-dimensional structure of the incubator of the utility model;

[0022] Figure 3 For this utility model Figure 2 A schematic diagram of the structure of the enlarged part A;

[0023] Figure 4 This is a schematic diagram of the half-section three-dimensional structure of the incubator of the present invention.

[0024] In the figure: 1. incubator; 2. fixed tray; 3. movable tray; 4. humidifier; 5. box door; 6. first screw; 7. first slider; 8. driving bevel gear; 9. driven bevel gear; 10. drive shaft; 11. groove; 12. fluorescent lamp; 13. second screw; 14. second slider; 15. drive motor; 16. rectangular groove; 17. fixed rod; 18. guide groove; 19. guide rod; 20. guide block; 21. hand wheel; 22. universal wheel; 23. brake assembly. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] See also Figure 1-4 A Dendrobium protocorm stem cell proliferation and culture system includes an incubator 1, a fixed tray 2, a movable tray 3, an adjustment device, a humidifier 4, a timed reciprocating device, and a moving device. The front end face of the incubator 1 is provided with a box door 5, and the back wall of the inner cavity of the incubator 1 is connected to the timed reciprocating device and the humidifier 4 is installed. Multiple groups of the fixed trays 2 are evenly distributed in the middle and lower parts of the incubator 1. The upper part of the incubator 1 is provided with the movable tray 3 through the adjustment device. The adjustment device includes a first screw 6, a first slider 7, an active bevel gear 8, a driven bevel gear 9 and a drive shaft 10. A groove 11 is provided on the upper side wall of one end of the incubator 1, and a rotating shaft 10 is provided in the groove 11. The first screw rod 6 is installed, and the first slider 7 is threadedly installed on the first screw rod 6. The side wall of the first slider 7 is fixedly connected to the side wall of the movable tray 3. A cavity is opened in the side wall of the incubator 1 near the end of the groove 11. The bottom end of the first screw rod 6 passes through the bottom wall of the groove 11 and extends to the cavity. The driven bevel gear 9 is sleeved therein. One end of the driven bevel gear 9 is meshed and connected with the active bevel gear 8. One end of the active bevel gear 8 passes through the side wall of the incubator 1 and is installed with the drive shaft 10. The fixed tray 2, the movable tray 3 and the top wall of the incubator 1 are all installed with fluorescent lamps 12. The bottom wall of the incubator 1 is installed with The mobile device, in this embodiment, when in use, the fixed trays 2 are distributed in the middle and lower part of the incubator 1, and are used to place the culture dishes of the Dendrobium protocorms that need to be cultivated. The positions of these trays are fixed, and are mainly used to provide a stable basic cultivation environment (the height is convenient for the operator to take and put in daily). When the upper movable tray 3 needs to be used, the drive shaft 10 is first rotated, and the drive shaft 10 drives the active bevel gear 8 to rotate. The active bevel gear 8 drives the driven bevel gear 9 to rotate during the rotation process. The driven bevel gear 9 drives the first screw 6 coaxially connected to the top to rotate in the rectangular groove 16. The first slider 7 on the first screw 6 moves up and down with the rotation of the first screw 6. Since the first slider 7 and the movable tray 3 are in contact with each other, the first slider 7 and the movable tray 3 are in contact with each other. The movable tray 3 is fixedly connected, so the movable tray 3 is also raised and lowered accordingly, realizing the height adjustment of the movable tray 3, making it convenient to place the culture dish of the Dendrobium protocorms to be cultured in the movable tray 3, and making it convenient for the staff to take and place the culture dish of the Dendrobium protocorms in the movable tray 3. The humidifier 4 is installed on the back wall of the incubator 1 and is controlled by a timed reciprocating device. The timed reciprocating device is started to drive the humidifier 4 to do reciprocating motion in the incubator 1, evenly distribute moisture, maintain a suitable humidity environment, turn on the fluorescent lamp 12, provide the required light intensity and light cycle for the Dendrobium, and promote its growth. The incubator 1 can be moved or fixed to the target position by the mobile device, so that the entire system can be flexibly moved and fixed in the laboratory or production site.

[0027] In actual use, it is further convenient for the humidifier 4 to evenly distribute the moisture. In this embodiment, the positioning reciprocating device includes a second screw 13, a second slider 14 and a drive motor 15. The back wall of the incubator 1 is provided with a rectangular groove 16. The rectangular groove 16 is rotatably installed with the second screw 13. The second screw 13 is threadedly installed with the second slider 14. The side wall of the second slider 14 is installed with the humidifier 4. The top of the second screw 13 passes through the top wall of the incubator 1 and the drive motor 15 is installed. The drive motor 15 drives the The second screw 13 rotates, causing the second slider 14 to perform reciprocating linear motion along the second screw 13 inside the incubator 1, so that the humidifier 4 installed on the second slider 14 can evenly distribute moisture inside the entire incubator 1, ensuring that the humidity conditions are consistent everywhere. The humidifier 4 can cover a larger area as the second slider 14 moves, avoiding the phenomenon of local humidity being too high or too low, ensuring that the Dendrobium protocorms grow under optimal humidity conditions, and ensuring that the Dendrobium protocorms obtain a uniform humidity environment during the proliferation and culture process, thereby improving their growth quality and efficiency.

[0028] During actual use, the second slider 14 is further guided. In this embodiment, two groups of fixing rods 17 are symmetrically installed in the rectangular groove 16 with the central axis of the second screw 13. The two groups of fixing rods 17 are slidingly connected to the second slider 14. The fixing rods 17 can provide additional support points for the second slider 14 to prevent it from lateral shaking during movement, thereby improving the overall stability of the system. Through the sliding connection between the fixing rods 17 and the second slider 14, it can be ensured that the second slider 14 moves in a straight line direction, reducing the error caused by the slider offset and improving the guiding accuracy. The sliding connection method can reduce the friction between the second slider 14 and the fixing rods 17, making the movement of the second slider 14 smoother and also reducing wear.

[0029] During actual use, the movement of the first slider 7 is further guided. In this embodiment, a guide groove 18 is provided on the side wall of the incubator 1 away from the groove 11, and a guide rod 19 is fixedly installed in the guide groove 18. The guide block 20 is slidably installed on the guide rod 19. The side wall of the guide block 20 is fixedly connected to the side wall of the movable tray 3 away from the first slider 7. The combination of the guide rod 19 and the guide block 20 can ensure that the movable tray 3 moves along a predetermined trajectory when moving, reduces shaking and deviation, and improves the overall stability of the system. By sliding the guide block 20 along the guide rod 19, it can be ensured that the movable tray 3 always remains parallel to the bottom surface of the incubator 1 during the movement.

[0030] During actual use, it is further convenient to drive the drive shaft 10. In this embodiment, a hand wheel 21 is provided on the end of the drive shaft 10. The hand wheel 21 allows the user to easily control the movement of the drive shaft 10 by rotating the hand wheel 21. Compared with directly rotating the drive shaft 10 by hand, the hand wheel 21 provides a greater torque, making the operation more labor-saving.

[0031] During actual use, the anti-slip property of the hand-cranked wheel 21 is further improved. In this embodiment, the outer wall of the hand-cranked wheel 21 is provided with anti-slip grooves. The anti-slip grooves can increase the friction coefficient of the surface of the hand-cranked wheel 21, making it easier for the operator to apply force when rotating the hand-cranked wheel 21, especially when the hands are wet or wearing gloves, a good grip can be maintained.

[0032] During actual use, the stability and accuracy of the operation of the drive motor 15 are further guaranteed. In this embodiment, the drive motor 15 is a servo motor. The servo motor can control the position, speed and torque with high precision. The servo motor adopts closed-loop control and has good stability. It can avoid problems such as stall and vibration, thereby ensuring the stability and accuracy of the operation of the drive motor 15.

[0033] During actual use, in order to further facilitate the movement and fixation of the incubator 1, in this embodiment, the moving device includes universal wheels 22 and brake assemblies 23. The universal wheels 22 are installed at the four corners of the bottom end of the incubator 1, and the brake assemblies 23 are provided on the universal wheels 22. The brake assemblies 23 are adapted to the universal wheels 22. Universal wheels 22 are installed at the four corners of the bottom end of the incubator 1. This can ensure the smooth movement of the incubator 1 in any direction, whether it is a straight line or a turn, and each universal wheel 22 should be provided with a brake assembly 23. The design of the brake assembly 23 needs to match the universal wheel 22, and can quickly lock the universal wheel 22 to prevent the incubator 1 from sliding when it does not need to move.

[0034] In order to explain in detail the possible application scenarios, technical principles, specific solutions that can be implemented, and the purpose and effects of this application, the following is a detailed description of the specific embodiments listed in conjunction with the accompanying drawings. The embodiments described herein are only used to more clearly illustrate the technical solutions of this application and are therefore only examples and are not intended to limit the scope of protection of this application.

[0035] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Dendrobium protocorm stem cell proliferation and culture system, comprising an incubator (1), a fixed tray (2), a movable tray (3), a regulating device, a humidifier (4), a timing reciprocating device, and a moving device, characterized in that: The front end face of the incubator (1) is provided with a door (5), the back wall of the inner cavity of the incubator (1) is provided with the humidifier (4) through the timing reciprocating device, a plurality of groups of fixed trays (2) are evenly distributed in the middle and lower parts of the incubator (1), the upper part of the incubator (1) is provided with the movable tray (3) through the adjusting device, the adjusting device comprising a first screw (6), a first slider (7), an active bevel gear (8), a driven bevel gear (9) and a drive shaft (10), a groove (11) is provided on the upper side wall of one end of the incubator (1), the first screw (6) is rotatably installed in the groove (11), and the first slider is threadedly installed on the first screw (6). (7), the side wall of the first slider (7) is fixedly connected to the side wall of the movable tray (3), a cavity is opened in the side wall of the incubator (1) near one end of the groove (11), the bottom end of the first screw (6) passes through the bottom wall of the groove (11) and extends to the cavity to be sleeved with the driven bevel gear (9), one end of the driven bevel gear (9) is meshedly connected with the driving bevel gear (8), one end of the driving bevel gear (8) passes through the side wall of the incubator (1) and is installed with the drive shaft (10), the fixed tray (2), the movable tray (3) and the top wall of the incubator are all installed with fluorescent lamps, and the bottom wall of the incubator (1) is installed with the moving device.

2. The Dendrobium protocorm stem cell proliferation and culture system according to claim 1, characterized in that: The positioning reciprocating device comprises a second screw (13), a second slider (14) and a driving motor (15); a rectangular groove (16) is provided on the back wall of the incubator (1); the second screw (13) is rotatably mounted in the rectangular groove (16); the second slider (14) is threadedly mounted on the second screw (13); the humidifier (4) is mounted on the side wall of the second slider (14); the top end of the second screw (13) passes through the top wall of the incubator (1) and is mounted with the driving motor (15).

3. The Dendrobium protocorm stem cell proliferation and culture system according to claim 2, characterized in that: Two groups of fixing rods (17) are symmetrically installed in the rectangular groove (16) with respect to the central axis of the second screw rod (13), and both groups of fixing rods (17) are slidably connected to the second sliding block (14).

4. The Dendrobium protocorm stem cell proliferation and culture system according to claim 3, characterized in that: A guide groove (18) is provided on the side wall of one end of the incubator (1) away from the groove (11), a guide rod (19) is fixedly installed in the guide groove (18), the guide block (20) is slidably installed on the guide rod (19), and the side wall of the guide block (20) is fixedly connected to the side wall of the movable tray (3) away from the first slider (7).

5. The Dendrobium protocorm stem cell proliferation and culture system according to claim 4, characterized in that: The end of the driving shaft (10) is sleeved with a hand-cranked wheel (21).

6. The Dendrobium protocorm stem cell proliferation and culture system according to claim 5, characterized in that: The outer wall of the hand-cranked wheel (21) is provided with anti-slip grooves.

7. The Dendrobium protocorm stem cell proliferation and culture system according to claim 6, characterized in that: The driving motor (15) is a servo motor.

8. The Dendrobium protocorm stem cell proliferation and culture system according to claim 7, characterized in that: The mobile device includes universal wheels (22) and brake components (23). The four corners of the bottom end of the incubator (1) are all equipped with the universal wheels (22). The brake components (23) are arranged on the universal wheels (22), and the brake components (23) are adapted to the universal wheels (22).

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