Reagent detection assembly

Abstract

The utility model belongs to the technical field of detection, and particularly relates to a reagent detection assembly which comprises a kit, the kit comprises a kit body, the kit body comprises at least two detection units, each detection unit comprises a sample adding pool, a detection area and a waste liquid pool which are sequentially connected, the sample adding pools, the detection areas and the waste liquid pools are communicated through channels, and each detection unit further comprises a first baffle and a second baffle. The first baffle is movably arranged between the sample adding pool and the detection area and is used for regulating and controlling connection or disconnection of the sample adding pool and the detection area; the second baffle is movably arranged between the detection area and the waste liquid pool and is used for regulating and controlling connection or disconnection of the detection area and the waste liquid pool. The device can be used for rapidly detecting various types to be detected at the same time, is convenient to wash and add liquid, and is simple to operate.

Description

Technical Field

[0001] The present application relates to the field of detection technology, and in particular to a reagent detection component. Background Art

[0002] Currently, the mainstream detection methods for furans, mycotoxins, heavy metals, food additive antigens, and illegal health product additive antigens include colloidal gold and enzyme-linked immunosorbent assays (ELISAs). These methods primarily rely on single-target detection. While the colloidal gold method is simple and quick, it has a high detection limit, low sensitivity, and can only perform qualitative analysis. While ELISAs are highly sensitive, the entire process requires the operator to constantly add liquids and wash, resulting in a long experimental cycle, time-consuming and labor-intensive, and numerous inconveniences. None of these methods meet the requirements for rapid on-site testing of food safety, drug safety, and feed safety, which require speed, efficiency, and cost-effectiveness.

[0003] Therefore, how to achieve a high degree of automation, fast and efficient operation in the process of simultaneous detection of multiple targets is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0004] In view of this, the main purpose of the present application is to provide a reagent detection component, which can quickly detect multiple antigens to be detected at the same time, is easy to wash and add liquid, and is simple to operate.

[0005] In order to achieve the above objectives, the technical solution adopted in this application is:

[0006] A reagent detection assembly includes a test kit, which includes a box body, which includes at least two detection units. The detection unit includes a sample loading pool, a detection area, and a waste liquid pool connected in sequence. The sample loading pool, the detection area, and the waste liquid pool are connected through a channel. The detection unit also includes a first baffle and a second baffle. The first baffle is movably arranged between the sample loading pool and the detection area to regulate the connection or disconnection between the sample loading pool and the detection area; the second baffle is movably arranged between the detection area and the waste liquid pool to regulate the connection or disconnection between the detection area and the waste liquid pool.

[0007] The channel includes a channel wall, the first baffle is rotatably arranged on the channel wall, or the first baffle is slidably arranged on the channel wall; the second baffle is rotatably arranged on the channel wall, or the second baffle is slidably arranged on the channel wall.

[0008] The detection area includes a biochip, and the upper surface of the biochip includes an antigen marker, a quality control marker and a control marker. The number of the antigen markers is at least two, and at least two of the antigen markers are arranged at intervals; the quality control marker includes a sheep anti-mouse polyclonal antibody or a rabbit anti-mouse polyclonal antibody.

[0009] In one embodiment of the present application, the upper surface of the biochip includes an antigen marking line, a quality control product marking line and a control line. The number of antigen marking lines is at least two, and at least two antigen marking lines are arranged at intervals; the quality control product marking line includes a sheep anti-mouse polyclonal antibody or a rabbit anti-mouse polyclonal antibody.

[0010] In another embodiment of the present application, the upper surface of the biochip includes at least two antigen spots, quality control spots, and blank control spots, which are spaced apart. The quality control spots are coated with goat anti-mouse polyclonal antibodies or rabbit anti-mouse polyclonal antibodies, and the blank control spots are coated with sample solution.

[0011] In the embodiment of the present application, the mark can be a marking line and / or a marking point, which can be produced by spraying, sprinkling or dotting.

[0012] The detection area includes a bottom plate, and the biochip is clamped on the bottom plate.

[0013] Among them, the box body also includes a washing liquid loading tank and a substrate liquid loading tank. Several first openings are set on the side wall of the washing liquid loading tank, and the washing liquid loading tank is used to load washing liquid into the sample loading tank through the first openings; several second openings are set on the side wall of the substrate liquid loading tank, and the substrate liquid loading tank is used to load substrate liquid into the sample loading tank through the second openings.

[0014] The box body further includes a button assembly, which is used to control the connection and disconnection between the first opening, the second opening and the sample addition pool.

[0015] The washing liquid carrying tank further comprises a plurality of first columns, a first door body and a plurality of first elastic members, wherein the plurality of first columns are arranged inside the cavity of the washing liquid carrying tank;

[0016] The first door body is used to close the first opening or open the first opening; a plurality of first elastic members are arranged between the first column and the first door body, and are used to give the first door body an elastic supporting force so that the first door body closes the first opening, and when the elastic members are compressed, the first door body opens the first opening.

[0017] The substrate liquid loading tank further comprises: a plurality of second columns, a second door, and a plurality of second elastic members. The plurality of second columns are disposed within the cavity of the washing liquid loading tank; the second door is used to close or open the second opening; and the plurality of second elastic members are disposed between the second columns and the second door, for providing elastic support to the second door, causing the second door to close the second opening and, when the elastic members are compressed, to open the second opening.

[0018] The box body further includes a third baffle, which is arranged between the sample loading pool and the channels close to the washing liquid loading pool and the substrate liquid loading pool.

[0019] Among them, the button assembly includes a supporting wall and a button body, a sliding groove is provided on the supporting wall, the button body is slidably set in the sliding groove, and is located between the first opening and the second opening, the first end of the button body is used to move toward the first door body and abut against the first door body, so that the first elastic member is in a compressed state, the first door body moves, so that the first door body opens; the second end of the button body is used to move toward the second door body and abut against the second door body, so that the second elastic member is in a compressed state, the second door body moves, so that the second door body opens; when the button body is in the middle position, there is a gap between the first end and the first door body, and there is a gap between the second end and the second door body.

[0020] Among them, a first arc-shaped protrusion and a second arc-shaped protrusion are provided on the side wall of the slide groove, and the first arc-shaped protrusion and the second arc-shaped protrusion are respectively located on both sides of the middle position of the button body; a limiting portion is provided on the side of the button body, and when the button body is subjected to a force toward or away from the first door body, the limiting portion can pass over the first arc-shaped protrusion; when the button body is subjected to a force toward or away from the second door body, the limiting portion can pass over the second arc-shaped protrusion.

[0021] The box body is circular, and a plurality of detection units extend from the central area to the circular edge.

[0022] Wherein, the washing liquid carrying pool and / or the substrate liquid loading pool are both annular, and the washing liquid carrying pool and the substrate liquid loading pool are arranged in an annular manner;

[0023] The button assembly is arranged between the washing liquid carrying tank and the substrate liquid loading tank; each detection unit corresponds to a button assembly.

[0024] Beneficial effects:

[0025] The reagent detection assembly provided in this application has a high degree of integration and can rapidly detect multiple species to be detected simultaneously. It can simultaneously detect multiple mycotoxins, heavy metals, food additives, or health product additives. It is also convenient for adding detergent and substrate solutions, simple to operate, with low reagent consumption, small sample usage, and fast analysis speed. The sample flow within the detection unit is controllable, and the reagent detection assembly is low-cost. It is particularly suitable for on-site rapid testing of food safety, drug safety, and feed safety, and can detect multiple targets simultaneously. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Schematic diagram of the planar structure of one embodiment of the box body of the kit of the present application;

[0027] Figure 2 for Figure 1 A schematic cross-sectional view of the first embodiment along line AA;

[0028] Figure 3 for Figure 1A schematic cross-sectional view of the second embodiment along line AA;

[0029] Figure 4 for Figure 1 A schematic cross-sectional view of the third embodiment along line AA;

[0030] Figure 5 for Figure 1 A schematic cross-sectional view of the fourth embodiment along line AA;

[0031] Figure 6 for Figure 1 A schematic cross-sectional view of the fifth embodiment along line AA;

[0032] Figure 7 for Figure 1 FIG. 1 is a schematic cross-sectional structural diagram of the sixth embodiment along line AA.

[0033] Figure 8 This is a structural diagram of an embodiment of a button assembly of the present application;

[0034] Figure 9 This is a schematic structural diagram of an embodiment of a biochip for the detection area of ​​the present application;

[0035] Figure 10 This is a schematic diagram of the upper surface structure of one embodiment of the kit of the present application.

[0036] Explanation of the reference numerals 100, box body; 110, detection unit; 111, sample loading tank; 112, detection area; 113, waste liquid tank; 114, channel; 115, first baffle; 116, second baffle; 117, sample loading port; 118, fifth baffle; 120, biochip; 121, antigen label; 122, quality control product label; 123, control label; 1121, bottom plate; 1122, buckle; 1123, observation port; 130, washing liquid loading tank; 131, first opening; 132, first column; 133, first column; A door body; 134, a first elastic member; 135, a first loading port; 140, a substrate liquid loading tank; 141, a second opening; 142, a second column; 143, a second door body; 144, a second elastic member; 145, a second loading port; 150, a third baffle; 160, a button assembly; 161, a supporting wall; 1611, a slide groove; 1612, a first arc-shaped protrusion; 1613, a second arc-shaped protrusion; 162, a button body; 1621, a limiting portion; 1622, a hand-held portion; 1623, a first end; 1624, a second end. DETAILED DESCRIPTION

[0037] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0038] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of this utility model, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0039] In this application, unless otherwise specified or limited, the terms "mounted," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection; direct connection or indirect connection through an intermediate medium; internal communication between two elements or interaction between two elements, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0040] In this application, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0041] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

[0042] The specific structure of the embodiment will be described in detail below with reference to the accompanying drawings.

[0043] like Figure 1 As shown, a reagent detection assembly includes a test kit, which includes a box body 100, and the box body 100 includes at least two detection units 110. The detection unit 110 includes a sample loading pool 111, a detection area 112 and a waste liquid pool 113 connected in sequence. The sample loading pool 111, the detection area 112 and the waste liquid pool 113 are connected through a channel 114. The detection unit 110 also includes a first baffle 115 and a second baffle 116. The first baffle 115 is movably arranged between the sample loading pool 111 and the detection area 112, and is used to regulate the connection or disconnection between the sample loading pool 111 and the detection area 112; the second baffle 116 is movably arranged between the detection area 112 and the waste liquid pool 113, and is used to regulate the connection or disconnection between the detection area 112 and the waste liquid pool 113.

[0044] In the embodiment of the present application, by providing a plurality of detection units 110 in the test kit, each detection unit 110 can independently detect the sample, so that a reagent detection component can detect multiple or multiple samples at the same time, which can improve the efficiency of the detection. In the embodiment of the present application, by providing a first baffle 115 and a second baffle 116, it is convenient to control the flow in the detection unit 110. When each detection unit 110 independently detects the sample, the liquid after the reaction is blocked and will not contaminate the detection area 112 after the reaction, making the detection process more accurate and reliable. At the same time, by controlling the opening and closing of the first baffle 115 and the second baffle 116, it is convenient to control the loading and flow direction of the washing liquid and the substrate liquid.

[0045] In one embodiment of the present application, Figure 2 and Figure 3As shown, the channel 114 includes a channel wall, a first baffle 115 is slidably set on the channel wall (not marked in the figure), and a second baffle 116 is slidably set on the channel wall. In an embodiment of the present application, the first baffle 115 and the second baffle 116 are both slidably set on the channel wall, so that in an embodiment of the present application, the connection and disconnection of the channel 114 are controlled by pulling the first baffle 115 and the second baffle 116. In an embodiment of the present application, the channel wall includes two channel side walls (not marked in the figure) arranged opposite to each other, and the first baffle 115 and the second baffle 116 are both slidably set on the two channel side walls. In other embodiments, such as Figure 4 As shown, a first baffle 115 is rotatably mounted on the channel wall; a second baffle 116 is rotatably mounted on the channel wall. For example, the first baffle 115 is rotatably mounted on two opposing sidewalls of the channel wall via a rotating shaft (not shown). When the first baffle 115 is perpendicular to the channel bottom wall (not shown), the first baffle 115 forms a seal with the channel bottom wall, channel sidewalls, and channel top wall (not shown), closing the first baffle 115. When the first baffle 115 is not perpendicular to the channel bottom wall, the first baffle 115 is opened. By controlling the angle between the first baffle 115 and the channel bottom wall, the flow rate of the liquid within the channel 114 can be controlled. A baffle knob (not shown) can be provided on the exterior of the channel sidewall, connected to the baffle's rotating shaft, to facilitate control of the rotation of the first baffle 115. The second baffle 116 is configured similarly to the first baffle 115 and will not be further explained here.

[0046] In one embodiment of the present application, the sample pool 111 stores a freeze-dried enzyme-labeled antibody mixture. During the reaction, a sample diluted with a sample diluent is added so that the sample in the sample pool 111 is mixed with the enzyme-labeled antibody, which facilitates reaching the detection area 112 for sample detection.

[0047] In one embodiment of the present application, Figure 5 and Figure 9 As shown, the detection area 112 includes a biochip 120. The upper surface of the biochip 120 includes an antigen marker 121, a quality control marker 122, and a control marker 123. The number of antigen markers 121 is at least two, and the at least two antigen markers 121 are spaced apart. The quality control marker 122 includes a goat anti-mouse polyclonal antibody or a rabbit anti-mouse polyclonal antibody. The control marker 123 serves as a detection background value. In the embodiment of the present application, the detection area 112 is provided with a biochip 120. By providing at least two antigen markers 121, the detection of at least two antigens can be achieved. The provision of the quality control marker 122 and the control marker 123 facilitates improved detection accuracy.

[0048] In the embodiment of the present application, the mark can be a marking line and / or a marking point, which can be produced by spraying, sprinkling or dotting.

[0049] In one embodiment of the present application, the upper surface of the biochip 120 includes an antigen marking line, a quality control product marking line and a control line. The number of antigen marking lines is at least two, and at least two antigen marking lines are arranged at intervals; the quality control product marking line includes a sheep anti-mouse polyclonal antibody or a rabbit anti-mouse polyclonal antibody.

[0050] In another embodiment of the present application, the top surface of the biochip 120 includes at least two antigen spots, a quality control spot, and a blank control spot, with the different spots spaced apart. The quality control spot is coated with a goat anti-mouse polyclonal antibody or a rabbit anti-mouse polyclonal antibody, and the blank control spot is coated with a sample solution. In other embodiments, the top surface of the biochip 120 may include antigen marker spots, quality control marker spots, and a control line. Alternatively, the top surface of the biochip 120 may include an antigen marker line, a quality control marker spot, and a control spot, etc.

[0051] In one embodiment of the present application, the control markers 122 on the top surface of the biochip 120 include three control markers 122, located at the upper left, lower left, and lower right corners of the biochip 120, respectively. There is one control marker 123, located at the upper right corner of the biochip 120. The control markers 122 and control marker 123 are located at a distance of ≥5 mm from the left and right edges of the biochip 120, and at a distance of ≥8 mm from the top and bottom edges of the biochip 120.

[0052] By adopting the above structure, the marginalization effect can be avoided after a series of reactions between the quality control marker 122, the control marker 123, and the antigen, so as to better control the quality of the kit and reduce the impact on the accuracy of the test results.

[0053] In one embodiment of the present application, the plurality of antigen markers 121 , the plurality of quality control markers 122 and the control marker 123 may be arranged in a matrix.

[0054] In one embodiment of the present application, the number of antigen markers 121 on the biochip 120 includes at least two, and each antigen marker 121 is coated with an antigen, and the specific number is based on the number of detection targets; the antigen markers 121, quality control product markers 122 and control markers 123 are arranged in a matrix form, and the shortest distance between any two points and the edge of the point is ≥700μm.

[0055] With the above structure, the antigen on each antigen marker 121 can fully react with the monoclonal antibody corresponding to the antigen in the dissolved enzyme-labeled antibody mixture, thereby obtaining a reaction result with a clear boundary and facilitating the determination of qualitative and quantitative detection results.

[0056] In the embodiments of the present application, the enzyme-labeled antibody mixture stored in the sample reservoir 111 reacts in a one-to-one correspondence with the antigens coated on the surface of the biochip 120 in the detection zone 112, and then undergoes a colorimetric reaction with the substrate in the substrate solution. The enzyme-labeled antibodies in the enzyme-labeled antibody mixture stored in the sample reservoir 111 and the antigens coated on the surface of the biochip 120 must be arranged in a corresponding manner. For ease of understanding, the present application provides the following specific embodiments.

[0057] When the reagent detection component is a mycotoxin reagent detection component, the antigen coated on the surface of the biochip 120 in the detection area 112 of each detection unit 110 in the test kit is any two or more of the following antigens: aflatoxin B1 antigen, vomitoxin antigen, zearalenone antigen, fumonisin antigen, ochratoxin A antigen, and T-2 toxin antigen, for example, any two, three, four, five, or six. The enzyme-labeled antibody mixture stored in the corresponding sample reservoir 111 contains any two or more of the following antigens: enzyme-labeled aflatoxin B1 monoclonal antibody, enzyme-labeled vomitoxin monoclonal antibody, enzyme-labeled zearalenone monoclonal antibody, enzyme-labeled fumonisin monoclonal antibody, enzyme-labeled ochratoxin A monoclonal antibody, and enzyme-labeled T-2 toxin monoclonal antibody, for example, any two, three, four, five, or six. The enzyme-labeled antibodies in the enzyme-labeled antibody mixture stored in the sample pool 111 should be set corresponding to the antigens coated on the surface of the biochip 120. For example, when the antigens coated on the surface of the biochip 120 are aflatoxin B1 antigen and vomitoxin antigen, the enzyme-labeled antibody mixture stored in the sample pool 111 contains enzyme-labeled aflatoxin B1 monoclonal antibody and enzyme-labeled vomitoxin monoclonal antibody.

[0058] When the reagent detection assembly is a heavy metal reagent detection assembly, the antigen coated on the surface of the biochip 120 in the detection area 112 of each detection unit 110 in the test kit is any two or three of the following: lead antigen, cadmium antigen, and mercury antigen. The corresponding enzyme-labeled antibody mixture stored in the sample reservoir 111 contains any two or three of the following: enzyme-labeled lead monoclonal antibody, enzyme-labeled cadmium monoclonal antibody, and enzyme-labeled mercury monoclonal antibody. For example, when the antigen coated on the surface of the biochip 120 in the detection area 112 is lead antigen and cadmium antigen, the enzyme-labeled antibody mixture stored in the sample reservoir 111 contains enzyme-labeled lead monoclonal antibody and enzyme-labeled cadmium monoclonal antibody.

[0059] When the reagent detection assembly is a food additive reagent detection assembly, the antigen coated on the surface of the biochip 120 in the detection area 112 of each detection unit 110 in the test kit is any two, three, or four of the following: sildenafil antigen, tadalafil antigen, glibenclamide antigen, and rosiglitazone antigen. The corresponding enzyme-labeled antibody mixture stored in the sample reservoir 111 contains any two, three, or four of the following: enzyme-labeled sildenafil monoclonal antibody, enzyme-labeled tadalafil monoclonal antibody, enzyme-labeled glibenclamide monoclonal antibody, and enzyme-labeled rosiglitazone monoclonal antibody. For example, when the antigen coated on the surface of the biochip 120 in the detection area 112 is sildenafil antigen, tadalafil antigen, and glibenclamide antigen, the enzyme-labeled antibody mixture stored in the sample reservoir 111 contains enzyme-labeled sildenafil monoclonal antibody, enzyme-labeled tadalafil monoclonal antibody, and enzyme-labeled glibenclamide monoclonal antibody.

[0060] When the reagent detection assembly is a health product additive reagent detection assembly, the antigen coated on the surface of the biochip 120 in the detection area 112 of each detection unit 110 in the test kit is any two, three, four, or five of the following: rosiglitazone antigen, glibenclamide antigen, sildenafil antigen, talaffil antigen, and diazepam antigen. The corresponding enzyme-labeled antibody mixture stored in the sample reservoir 111 contains any two, three, four, or five of the following: enzyme-labeled rosiglitazone monoclonal antibody, enzyme-labeled glibenclamide monoclonal antibody, enzyme-labeled sildenafil monoclonal antibody, enzyme-labeled talaffil monoclonal antibody, and enzyme-labeled diazepam monoclonal antibody. The enzyme-labeled antibodies in the enzyme-labeled antibody mixture stored in the sample reservoir 111 and the antigen coated on the surface of the biochip 120 should be set correspondingly. For example, when the antigens coated on the surface of the biochip 120 in the detection area 112 of each detection unit 110 in the kit are rosiglitazone antigen and glibenclamide antigen, the enzyme-labeled antibody mixture stored in the sample pool 111 contains enzyme-labeled rosiglitazone monoclonal antibody and enzyme-labeled glibenclamide monoclonal antibody.

[0061] The waste liquid pool 113 collects the reacted sample, washing liquid, and substrate liquid in sequence, and can accommodate a volume of at least 0.5 ml, preferably 0.5 ml to 2 ml.

[0062] In one embodiment of the present application, the detection area 112 includes a base plate 1121, and the biochip 120 is snapped onto the base plate 1121. In one embodiment of the present application, the biochip 120 is snapped onto the base plate 1121 via the snap 1122. This allows the biochip 120 to be removed from the detection area 112 after the reaction in the detection area 112 is completed, allowing it to be placed in a scanner for scanning and statistical analysis of the test results to obtain a quantitative result. Furthermore, during testing, the biochip 120 can be easily secured to the base plate 1121. In other embodiments, the biochip 120 and the base plate 1121 may be secured in other removable manners.

[0063] In one embodiment of the present application, Figure 1 、 Figure 5 and Figure 7 As shown, the box body 100 also includes a washing liquid loading pool 130 and a substrate liquid loading pool 140. A plurality of first openings 131 are provided on the side wall of the washing liquid loading pool 130, and the washing liquid loading pool 130 is used to load washing liquid into the sample loading pool 111 through the first openings 131; a plurality of second openings 141 are provided on the side wall of the substrate liquid loading pool 140, and the substrate liquid loading pool 140 is used to load substrate liquid into the sample loading pool 111 through the second openings 141.

[0064] In the embodiment of the present application, by providing a washing liquid loading pool 130 and a substrate liquid loading pool 140 on the box body 100 , the integration level of the box body 100 is improved, and the washing liquid and substrate liquid are easily input into the detection unit 110 .

[0065] In one embodiment of the present application, Figure 1 、 Figure 6-Figure 8 As shown, the box body 100 also includes a button assembly 160, which is used to control the connection and disconnection between the first opening 131, the second opening 141 and the sample addition pool 111. In the embodiment of the present application, by providing the button assembly 160, it is convenient to control the connection and disconnection between the washing liquid carrying pool 130, the substrate liquid loading pool 140 and the sample addition pool 111, so as to facilitate the control of adding washing liquid and substrate liquid to the sample addition pool 111. At the same time, when washing liquid and substrate liquid are not needed, the first opening 131 and the second opening 141 can also be controlled to be closed so that the washing liquid and substrate liquid cannot be circulated to the sample addition pool 111. By providing the button assembly 160, the addition of washing liquid and substrate liquid can be achieved through a switch, which simplifies the operation.

[0066] In one embodiment of the present application, the washing liquid carrying tank 130 further includes a plurality of first cylinders 132, a first door body 133 and a plurality of first elastic members 134, wherein the plurality of first cylinders 132 are arranged inside the cavity of the washing liquid carrying tank 130; the first door body 133 is used to close the first opening 131 or open the first opening 131; and the plurality of first elastic members 134 are arranged between the first cylinder 132 and the first door body 133, and are used to give the first door body 133 an elastic supporting force so that the first door body 133 closes the first opening 131, and when the elastic member is compressed, the first door body 133 opens the first opening 131. In an embodiment of the present application, the first elastic member 134 may be a spring, and in other embodiments, the first elastic member 134 may also be other elastic materials. In an embodiment of the present application, as Figure 5 As shown, when the button assembly 160 exerts a force on the first door 133, and the force is at least opposite to the force exerted by the spring on the first door 133, and the force is greater than the force exerted by the spring on the first door 133, the first door 133 can be opened, so that the washing liquid in the washing liquid holding tank 130 flows into the channel 114 and further flows into the sample loading tank 111. Figure 3 and Figure 6 As shown, when the first door body 133 is not subjected to the force applied to the first door body 133 by the button assembly 160, or the force applied to the first door body 133 is small, the first door body 133 closes the first opening 131, so that the washing liquid cannot continue to flow to the channel 114, and thus cannot flow to the sample loading pool 111. In the embodiment of the present application, the first door body 133 is a whole door body, and the first column 132 is arranged on the bottom wall inside the cavity of the washing liquid holding pool 130. In other embodiments, the first column 132 can also be arranged on the top wall inside the cavity of the washing liquid holding pool 130 to facilitate the outflow of the washing liquid. The first door body 133 can also be composed of two sub-door bodies merged together to close the first opening 131. When the sub-door body is opened, the first opening 131 is opened.

[0067] In one embodiment of the present application, the substrate liquid loading tank 140 further includes: a plurality of second columns 142, a second door 143, and a plurality of second elastic members 144. The plurality of second columns 142 are disposed inside the cavity of the washing liquid loading tank 130; the second door 143 is used to close the second opening 141 or open the second opening 141; and the plurality of second elastic members 144 are disposed between the second columns 142 and the second door 143 to provide the second door 143 with elastic support force, so that the second door 143 closes the second opening 141. Figure 6As shown, when the elastic member is compressed, the second door 143 opens the second opening 141. In the embodiment of the present application, the arrangement of the plurality of second columns 142, the second door 143 and the plurality of second elastic members 144 is similar to that of the first columns 132, the first door 133 and the plurality of first elastic members 134, and will not be further elaborated here.

[0068] In one embodiment of the present application, the cartridge 100 further includes a third baffle 150, which is disposed between the sample reservoir 111 and the channel 114 near the wash solution reservoir 130 and the substrate solution loading reservoir 140. In this embodiment of the present application, the provision of the third baffle 150 further includes the sample reservoir 111, preventing residual wash solution and substrate solution in the channel 114 from reaching the sample reservoir 111 when the wash solution and substrate solution are no longer needed. This further protects the sample reservoir 111.

[0069] In one embodiment of the present application, Figure 5-6 、 Figure 8 As shown, the button assembly 160 includes a support wall 161 and a button body 162. A slide groove 1611 is provided on the support wall 161. The button body 162 is slidably provided in the slide groove 1611 and is located between the first opening 131 and the second opening 141. The first end 1623 of the button body 162 is used to move toward the first door body 133 and abut against the first door body 133, so that the first elastic member 134 is in a compressed state. The first door body 133 moves to open the first door body 133. At this time, the second door body 143 is in a state of closing the second opening 141. The second end 1624 of the button body 162 is used to move toward the second door body 143 and abut against the second door body 143, so that the second elastic member 144 is in a compressed state. The second door body 143 moves to open the second door body 143. At this time, the first door body 133 is in a state of closing the first opening 131; as shown Figure 3 As shown, when the button body 162 is in the middle position, there is a gap between the first end 1623 and the first door body 133, and there is a gap between the second end 1624 and the second door body 143. At this time, the first door body 133 closes the first opening 131, and the second door body 143 closes the second opening 141. In the embodiment of the present application, by pushing the button body 162 back and forth, it is convenient to control the first end 1623 and the second end 1624 of the button body 162 to abut against the first door body 133 and the second door body 143 to control the closing and opening of the first opening 131 and the second opening 141.

[0070] In one embodiment of the present application, Figure 8As shown, a first arc-shaped protrusion 1612 and a second arc-shaped protrusion 1613 are provided on the side wall of the slide groove 1611, and the first arc-shaped protrusion 1612 and the second arc-shaped protrusion 1613 are respectively located on both sides of the middle position of the button body 162; a limiting portion 1621 is provided on the side of the button body 162, and when the button body 162 is subjected to a force in the direction toward or away from the first door body 133, the limiting portion 1621 can pass over the first arc-shaped protrusion 1612; when the button body 162 is subjected to a force in the direction toward or away from the second door body 143, the limiting portion 1621 can pass over the second arc-shaped protrusion 1613. In the embodiment of the present application, the first arc-shaped protrusion 1612 is arranged on the side close to the first door body 133. By setting the first arc-shaped protrusion 1612 and the limiting portion 1621, when the limiting portion 1621 moves toward the first door body 133, the limiting portion 1621 reaches the first arc-shaped protrusion 1612. When subjected to an applied force, the limiting portion 1621 can pass over the first arc-shaped protrusion 1612, so that the first end 1623 abuts against the first door body 133 and opens the first door body 133, so that when no external force is applied, the first door body 133 can remain in an open state, thereby improving the use effect of the button assembly 160 and reducing the duration of application of the force. Similarly, the second arc-shaped protrusion 1613 is provided on the side close to the second door body 143. By providing the second arc-shaped protrusion 1613 and the limiting portion 1621, when the limiting portion 1621 moves toward the second door body 143, the limiting portion 1621 reaches the second arc-shaped protrusion 1613. When subjected to an applied force, the limiting portion 1621 can pass over the second arc-shaped protrusion 1613, so that the second end 1624 abuts against the second door body 143 and opens the second door body 143. When no external force is applied, the second door body 143 can remain open, thereby improving the use effect of the button assembly 160 and reducing the duration of the applied force. In the embodiment of the present application, the limiting portion 1621 has at least an arc structure, which is convenient for passing over the first arc-shaped protrusion 1612 and the second arc-shaped protrusion 1613. In the embodiment of the present application, the limiting portion 1621 is simultaneously slidably provided in the slide groove 1611 to facilitate the sliding of the button body 162.

[0071] In one embodiment of the present application, the button body 162 includes a handle 1622 , so that the operator can control the handle 1622 to push the button body 162 .

[0072] In one embodiment of the present application, Figure 1As shown, the box body 100 is circular, with several detection units 110 extending from the center area toward the circular edge. In the embodiment of the present application, by configuring the box body 100 in a circular shape, it is convenient to install multiple detection units 110. The edges exert centrifugal force on the box body 100, thereby improving the detection effect. In the embodiment of the present application, the number of detection units 110 is four. In other embodiments, the number of detection units 110 can also be six, eight, nine, etc.

[0073] In one embodiment of the present application, the washing liquid loading pool 130 is circular, and the substrate liquid loading pool 140 is annular, and the washing liquid loading pool 130 and the substrate liquid loading pool 140 are arranged in an annular arrangement. In one embodiment of the present application, the substrate liquid loading pool 140 is arranged in an annular arrangement around the washing liquid loading pool. In other embodiments, it can also be that the substrate liquid loading pool 140 is circular, and the washing liquid loading pool 130 is annular, and the washing liquid loading pool 130 is arranged in an annular arrangement around the substrate liquid. It can also be that the washing liquid loading pool 130 and the substrate liquid loading pool 140 are both annular, and both are arranged in an annular arrangement.

[0074] In one embodiment of the present application, a button assembly 160 is disposed between the wash liquid holding reservoir 130 and the substrate liquid loading reservoir 140; each detection unit 110 corresponds to a button assembly 160. In this embodiment of the present application, the wash liquid holding reservoir 130 and the substrate liquid loading reservoir 140 are arranged in an annular manner with a gap between them. By providing multiple button assemblies 160, it is convenient to control the application of wash liquid and substrate liquid to multiple detection units 110. In this embodiment of the present application, one wash liquid holding reservoir 130 can input wash liquid to multiple detection units 110, and one substrate liquid loading reservoir 140 can input substrate liquid to multiple detection units 110.

[0075] In other embodiments, a plurality of partitions may be provided in the washing liquid loading pool 130 and the substrate liquid loading pool 140, so that the washing liquid loading pool 130 is divided into a plurality of sub-washing liquid loading pools 130, and the substrate liquid loading pool 140 is divided into a plurality of sub-substrate liquid loading pools 140, each sub-washing liquid loading pool 130 and each sub-substrate liquid loading pool 140 corresponds to one detection unit 110.

[0076] In another embodiment, the washing liquid loading reservoir 130 and the substrate liquid loading reservoir 140 may not be provided on the box body 100. The reagent detection assembly is provided with a washing liquid reagent bottle and a substrate liquid bottle, wherein the washing liquid reagent bottle contains washing liquid and the substrate liquid bottle contains substrate liquid to load the washing liquid and substrate liquid into the sample loading reservoir 111.

[0077] In one embodiment of the present application, Figure 1 and Figure 10As shown, the sample addition pool 111 is provided with a sample addition port 117 for easy sample addition, and a sealing film can be provided to seal the sample addition port 117. The freeze-dried enzyme-labeled antibody mixture is sealed and stored by the sealing film to prevent accidental spillage.

[0078] In one embodiment of the present application, an observation port 1123 is provided at a corresponding position of the detection zone 112, preferably an opening sealed with a transparent sealing film. This facilitates intuitive observation of the test results, facilitates semi-quantitative determination, and prevents accidental spillage of the reaction solution that could affect the accuracy of the test results.

[0079] In one embodiment of the present application, the washing liquid loading tank 130 is provided with a first loading port 135 and the substrate liquid loading tank 140 is provided with a second loading port 145 to facilitate loading of additional washing liquid into the washing liquid loading tank 130 and loading of additional substrate liquid into the substrate loading tank.

[0080] In one embodiment of the present application, Figure 1-2 and Figure 10 As shown, one end of the waste liquid pool 113 away from the detection area 112 is connected to the channel 114. A fifth baffle 118 is provided on the channel 114 at the end of the waste liquid pool 113 away from the detection area 112. By controlling the opening of the fifth baffle 118, the discharge of waste liquid from the waste liquid pool 113 is controlled. The fifth baffle 118 is closed by the gap, so that the waste liquid in the waste liquid pool 113 cannot pass through the channel 114 at the location of the fifth baffle 118.

[0081] In the embodiment of the present application, the cross-section of the channel 114 can be circular, square, or other shapes. A circular shape is preferred, as it allows the liquid to flow more smoothly from the sample reservoir 111 to the detection zone 112 and from the detection zone 112 to the waste liquid reservoir 113 by centrifugation, and prevents residual liquid from remaining in the channel.

[0082] In the embodiment of the present application, the working process of the kit may include the following steps:

[0083] In step 1, a sample diluted with a sample diluent is added to the sample loading reservoir 111, and the first baffle 115 is removed. The sample diluted with the diluent and the enzyme-labeled antibody mixture dissolved in the sample loading reservoir 111 can be mixed by centrifugation and then transported together to the detection area 112. The first baffle 115 is then installed to allow the sample to fully react in the detection area 112. The second baffle 116 is removed, and the reacted sample and enzyme-labeled antibody mixture can be transported to the waste liquid reservoir 113 by centrifugation, and the second baffle 116 is then installed. In other embodiments, the fifth baffle 118 can also be removed at the same time as the second baffle 116 is removed, so that the reacted sample and enzyme-labeled antibody mixture can be directly transported to the outside of the box body 100, reducing the risk of waste liquid in the waste liquid reservoir 113 contaminating unused detection units.

[0084] Step 2: Open the first baffle 115 and the third baffle 150. Push the handle 1622 of the button body 162 so that the first end 1623 of the button body 162 abuts the first door 133, allowing the washing liquid in the washing and loading tank to flow into the channel 114 and further to the sample loading tank 111. The button body 162 is restored to the middle position, and the washing liquid can be transported to the detection area 112 by centrifugal action. The first baffle 115 is then installed to allow sufficient reaction in the detection area 112. The second baffle 116 is opened to transport the washing liquid to the waste liquid tank 113 by centrifugal action. The second baffle 116 is then installed. In other embodiments, the fifth baffle 118 can also be opened simultaneously with the opening of the second baffle 116 to facilitate direct delivery of the washing liquid to the outside of the box body 100.

[0085] Step 3, open the first baffle 115 and the third baffle 150, and push the hand-held part 1622 of the button body 162 in the reverse direction so that the second end 1624 of the button body 162 abuts the second door body 143, so that the substrate liquid in the substrate liquid loading tank 140 flows into the channel 114 and further reaches the sample addition tank 111, and restore the button body 162 to the middle position. The substrate liquid can be transported to the detection area 112 by centrifugation, and then the first baffle 115 is installed to allow it to fully react in the detection area 112; remove the second baffle 116, and then the washing liquid can be transported to the waste liquid tank 113 by centrifugation, and then the second baffle 116 is installed.

[0086] Through the above reaction, multiple antigens in the sample are detected at the same time, and the reagent consumption is low and the sample amount is small, making the detection process more convenient, faster and less costly. The above steps of this application are only examples and do not limit the specific steps.

[0087] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application shall be included in the scope of protection of the present application.

Claims

1. A reagent detection component, characterized in that: The invention comprises a test kit, which comprises a box body, which comprises at least two detection units, wherein the detection unit comprises a sample loading pool, a detection area and a waste liquid pool connected in sequence, wherein the sample loading pool, the detection area and the waste liquid pool are connected through a channel, and the detection unit further comprises a first baffle and a second baffle, wherein the first baffle is movably arranged between the sample loading pool and the detection area, and is used to regulate the connection or disconnection between the sample loading pool and the detection area; and the second baffle is movably arranged between the detection area and the waste liquid pool, and is used to regulate the connection or disconnection between the detection area and the waste liquid pool.

2. The reagent detection assembly according to claim 1, characterized in that The channel includes a channel wall, and the first baffle is rotatably disposed on the channel wall, or the first baffle is slidably disposed on the channel wall; The second baffle is rotatably arranged on the channel wall, or the second baffle is slidably arranged on the channel wall.

3. The reagent detection assembly according to claim 1, characterized in that: The detection area includes a biochip, the upper surface of which includes an antigen marker, a quality control marker and a control marker. The number of the antigen markers is at least two, and at least two of the antigen markers are arranged at intervals; the quality control marker includes a goat anti-mouse polyclonal antibody or a rabbit anti-mouse polyclonal antibody.

4. The reagent detection assembly according to claim 3, characterized in that: The detection area includes a bottom plate, and the biochip is engaged with the bottom plate.

5. The reagent detection assembly according to claim 1, characterized in that: The box body also includes: A washing liquid carrying tank, wherein a plurality of first openings are provided on the side wall of the washing liquid carrying tank, and the washing liquid carrying tank is used to load washing liquid into the sample loading tank through the first openings; The substrate liquid loading pool has a plurality of second openings on its side wall, and the substrate liquid loading pool is used to load the substrate liquid into the sample addition pool through the second openings.

6. The reagent detection assembly according to claim 5, characterized in that: The box body further includes a button assembly, and the button assembly is used to control the connection and disconnection between the first opening, the second opening and the sample loading reservoir.

7. The reagent detection assembly according to claim 6, characterized in that: The washing liquid carrying tank also includes: a plurality of first columns, disposed inside the cavity of the washing liquid carrying tank; a first door body, used for closing the first opening or opening the first opening; a plurality of first elastic members, disposed between the first column and the first door body, for providing elastic support force to the first door body so that the first door body closes the first opening; when the elastic members are compressed, the first door body opens the first opening; and / or, The substrate liquid loading tank also includes: a plurality of second columns, disposed inside the cavity of the washing liquid carrying tank; a second door body, used for closing the second opening or opening the second opening; a plurality of second elastic members, disposed between the second column and the second door body, for providing an elastic supporting force to the second door body so that the second door body closes the second opening, and when the elastic members are compressed, the second door body opens the second opening; and / or The box body further includes a third baffle, which is arranged between the channels of the sample loading reservoir close to the washing liquid carrying reservoir and the substrate liquid loading reservoir.

8. The reagent detection assembly according to claim 7, characterized in that: The button assembly includes: A supporting wall, wherein a slide groove is provided on the supporting wall. The button body is slidably arranged in the sliding groove and is located between the first opening and the second opening. The first end of the button body is used to move toward the first door body and abut against the first door body, so that the first elastic member is in a compressed state and the first door body moves to open the first door body; the second end of the button body is used to move toward the second door body and abut against the second door body, so that the second elastic member is in a compressed state and the second door body moves to open the second door body; when the button body is in the middle position, there is a gap between the first end and the first door body, and there is a gap between the second end and the second door body.

9. The reagent detection assembly according to claim 8, characterized in that: A first arc-shaped protrusion and a second arc-shaped protrusion are provided on the side wall of the slide groove, and the first arc-shaped protrusion and the second arc-shaped protrusion are respectively located on both sides of the middle position of the button body; A limiting portion is provided on the side of the button body. When the button body is subjected to a force in a direction toward or away from the first door body, the limiting portion can pass over the first arc-shaped protrusion; when the button body is subjected to a force in a direction toward or away from the second door body, the limiting portion can pass over the second arc-shaped protrusion.

10. The reagent detection assembly according to claim 6, characterized in that: The box body is circular, and the plurality of detection units extend from the central area to the circular edge; The washing liquid carrying pool and / or the substrate liquid loading pool are both annular, and the washing liquid carrying pool and the substrate liquid loading pool are arranged in an annular manner; The button assembly is arranged between the washing liquid carrying tank and the substrate liquid loading tank; each detection unit corresponds to a button assembly.

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