Separation culture device for primary cells of lung cancer

Abstract

The utility model discloses a lung cancer primary cell separation culture device, which relates to the technical field of lung cancer primary cell culture and comprises a culture box, a heating plate is mounted at the lower end of the culture box, a sealing cover is mounted at the upper end of the culture box, a through groove is formed in the upper end of the sealing cover, and an opening and closing door is rotatably connected to the surface of the through groove. A rotating shaft is rotationally connected to the center position in the incubator, a driving device is connected to the position, located in the incubator, of the lower end of the rotating shaft, a rotating table is fixedly mounted on the surface of the rotating shaft, a plurality of mounting grooves are formed in the surface of the rotating table in an array mode, and opening grooves are formed in the center positions of the bottoms of the mounting grooves; an electric push rod is installed in the opening groove, and a push plate is installed at the upper end of the electric push rod. By arranging a series of structures, in the taking process, the whole process is mechanically controlled, compared with manual taking, the stability is higher, contact with other culture dishes is avoided in the taking process, and safety and rapidness are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of primary lung cancer cell culture, in particular to a primary lung cancer cell separation and culture device. Background Art

[0002] Primary lung cancer cells refer to lung cancer cells that are cultured immediately after being removed from the body. When performing cell culture, culture dishes are usually used for culture.

[0003] When cells are cultured in culture dishes, multiple control groups are established for experimental comparison. Therefore, the environmental data of the control groups must be consistent to avoid the environmental data affecting the experimental data. Multiple control groups are placed together, making it inconvenient to take out a culture dish individually for observation and replacement. In addition, when the culture dish is taken out manually, improper operation can easily damage the culture dish, causing the nutrient solution inside the culture dish to come into contact with other culture dishes, resulting in data confusion. Utility Model Content

[0004] The purpose of the present invention is to provide a device for separating and culturing primary lung cancer cells to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device for separating and culturing primary lung cancer cells, comprising an incubator, a heating plate installed at the lower end of the incubator, a sealing cover installed at the upper end of the incubator, a through groove provided at the upper end of the sealing cover, an opening and closing door rotatably connected to the surface of the through groove, a rotating shaft rotatably connected at the inner center position of the incubator, the lower end of the rotating shaft is located at a position inside the incubator and is connected to a driving device, a rotating table is fixedly installed on the surface of the rotating shaft, a surface array of the rotating table is provided with a plurality of mounting grooves, an open groove is provided at the bottom center position of the mounting groove, an electric push rod is installed inside the open groove, a push plate is installed at the upper end of the electric push rod, a lifting platform is installed at the upper end of the push plate, the lifting platform is slidably connected to the inside of the mounting groove, a culture dish is placed on the upper end of the lifting platform, and the size of the culture dish is smaller than the size of the through groove.

[0006] Preferably, a control platform is installed at the front end of the incubator, a display screen is provided on the surface of the control platform, and a plurality of control components are installed on the surface of the control platform below the display screen, and each of the control components is electrically connected to an electric push rod.

[0007] Preferably, the control assembly includes an ascending control button and a descending control button, and the ascending control button and the descending control button are both electrically connected to the electric push rod.

[0008] Preferably, a temperature sensor is installed inside the installation slot, and a plurality of the temperature sensors are electrically connected to the display screen.

[0009] Preferably, a plurality of grooves are arranged in an array on the side wall surface of the mounting groove, and guide rods are installed inside the grooves. The plurality of guide rods are fixedly connected to the lifting platform, and the upper ends of the guide rods pass through the grooves through holes and extend to the top of the rotating table.

[0010] Preferably, the bottom array of the incubator is provided with a plurality of ventilation holes, the ventilation holes are located between the rotating platform and the side wall of the incubator, and the side wall surface of the cover is provided with a plurality of ventilation holes.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] 1. This primary lung cancer cell separation and culture device has an installation slot opened inside the incubator, and an electric push rod and a push plate are installed inside the installation slot. The electric push rod drives the push plate and the lifting platform to move up and down, and the lifting platform can drive the culture dish to move in the vertical direction. When the user needs to take out the culture dish, the door is opened, and the drive device is started to drive the rotating shaft and the rotating table to rotate. The installation slot is rotated below the through slot, and the electric push rod drives the lifting platform and the culture dish to rise until the culture dish passes through the through slot. At this time, the culture dish can be taken out. During the process of taking out, the whole process is mechanically controlled. Compared with manual taking out, it has higher stability and will not come into contact with other culture dishes during the taking out process, which is safer and faster.

[0013] 2. This primary lung cancer cell isolation and culture device installs a heating plate at the lower end of the incubator. The heating plate is connected to an external power supply. The incubator can be heated by the heating plate, making it convenient for the user to change the temperature inside the incubator. Multiple temperature sensors can detect temperature changes inside the installation tank in real time, making it convenient for the user to record experimental data. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a structural diagram of the overall structure of the utility model;

[0015] Figure 2 It is a structural diagram of the internal structure of the utility model;

[0016] Figure 3 This is a schematic diagram of the structure of the control assembly of the utility model;

[0017] Figure 4 This is a structural diagram of the lifting platform of the utility model;

[0018] Figure 5 For this utility model Figure 4 A magnified view of middle A;

[0019] Figure 6 This is a structural diagram of the lifting rod of the utility model.

[0020] In the figure: 1. Incubator; 2. Heating plate; 3. Control platform; 4. Cover; 5. Opening and closing door; 6. Ventilation port; 7. Through slot; 8. Rotating shaft; 9. Culture dish; 10. Control assembly; 1001. Up control button; 1002. Down control button; 11. Rotating table; 12. Lifting platform; 13. Ventilation port; 14. Groove; 15. Guide rod; 16. Mounting slot; 17. Electric push rod; 18. Push plate; 19. Display screen. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," "the other end," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They are not intended to indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0023] like Figures 1 to 6As shown, the primary lung cancer cell separation and culture device of this embodiment includes an incubator 1, a heating plate 2 is installed at the lower end of the incubator 1, and the heating plate 2 is connected to an external power supply to change the temperature inside the incubator 1, and a cover 4 is installed at the upper end of the incubator 1, and the cover 4 is fixedly installed at the upper end of the incubator 1, and a through groove 7 is opened at the upper end of the cover 4, and an opening and closing door 5 is rotatably connected to the surface of the through groove 7, and the opening and closing door 5 needs to be manually closed or opened, and a rotating shaft 8 is rotatably connected to the center position of the interior of the incubator 1, and the lower end of the rotating shaft 8 is located at a position inside the incubator 1 and is connected to a driving device, which is a common servo motor on the market, which can drive the rotating shaft 8 to rotate and is connected to an external power supply. A rotating table 11 is fixedly installed on the surface of the rotating shaft 8, and the upper end of the rotating shaft 8 is rotatably connected to the lower end of the cover 4, and the rotating table 11 can be driven to rotate by the rotating shaft 8, and the lower end of the rotating table 11 is rotatably connected to the inner bottom end of the incubator 1, and the surface of the rotating table 11 The array is provided with a plurality of mounting slots 16, and an open slot is provided at the bottom center position of the mounting slot 16, and an electric push rod 17 is installed inside the open slot, and the electric push rod 17 is connected to an external power supply, and a push plate 18 is installed on the upper end of the electric push rod 17. Under normal conditions, the push plate 18 and the electric push rod 17 are both located inside the open slot, and a lifting platform 12 is installed on the upper end of the push plate 18, which can drive the push plate 18 and the lifting platform 12 to move up and down through the electric push rod 17, and the lifting platform 12 is slidably connected to the inside of the mounting slot 16. A culture dish 9 is placed on the upper end of the lifting platform 12, and the size of the culture dish 9 is smaller than the size of the through-slot 7. When the user needs to take out the culture dish 9, the opening and closing door 5 is opened, and the driving device is started to drive the rotating shaft 8 and the rotating table 11 to rotate, and the mounting slot 16 is rotated to the bottom of the through-slot 7, and the lifting platform 12 and the culture dish 9 are driven to rise by the electric push rod 17 until the culture dish 9 passes through the through-slot 7, at which time the culture dish 9 can be taken out.

[0024] Specifically, a control platform 3 is installed at the front end of the incubator 1, and a display screen 19 is provided on the surface of the control platform 3. A plurality of control components 10 are installed on the surface of the control platform 3 below the display screen 19. Each control component 10 is electrically connected to an electric push rod 17. The control component 10 includes an upward control button 1001 and a downward control button 1002. The upward control button 1001 and the downward control button 1002 are both electrically connected to the electric push rod 17. The number of control components 10 is consistent with the number of electric push rods 17. Each control component 10 can control an electric push rod 17 to perform independent lifting and lowering movements. The upward control button 1001 and the downward control button 1002 can control the electric push rod 17 to perform upward or downward movements.

[0025] Furthermore, a temperature sensor is installed inside the mounting groove 16, and multiple temperature sensors are electrically connected to the display screen 19. By installing a heating plate 2 at the lower end of the incubator 1, the heating plate 2 is connected to an external power supply, and the incubator 1 can be heated by the heating plate 2, which is convenient for the user to change the temperature inside the incubator 1. The temperature changes inside the mounting groove 16 can be detected in real time through multiple temperature sensors, which is convenient for the user to record experimental data.

[0026] Furthermore, a plurality of grooves 14 are provided in an array on the side wall surface of the mounting groove 16, and guide rods 15 are installed inside the grooves 14. The plurality of guide rods 15 are fixedly connected to the lifting platform 12. The upper ends of the guide rods 15 pass through the grooves 14 through holes and extend to the top of the rotating table 11. The plurality of guide rods 15 will move synchronously with the lifting platform 12. The surfaces of the guide rods 15 and the holes are slidably connected. The moving trajectory of the lifting platform 12 can be limited and guided by the plurality of guide rods 15.

[0027] Furthermore, a plurality of ventilation holes 13 are provided in an array at the bottom of the incubator 1, and the ventilation holes 13 are located between the rotating table 11 and the side wall of the incubator 1. A plurality of ventilation holes 6 are provided on the side wall surface of the cover 4. The incubator 1 needs to be placed in a sterile environment for cultivation. The ventilation holes 6 can facilitate the entry of external sterile air into the interior of the incubator 1. The plurality of ventilation holes 13 can facilitate the transfer of heat, making it convenient for the heating plate 2 to change the temperature inside the incubator 1.

[0028] The method of using this embodiment is as follows: by opening a mounting groove 16 inside the incubator 1, an electric push rod 17 and a push plate 18 are installed inside the mounting groove 16, and the electric push rod 17 drives the push plate 18 and the lifting platform 12 to move up and down, and the lifting platform 12 can drive the culture dish 9 to move in the vertical direction. When the user needs to take out the culture dish 9, the opening and closing door 5 is opened, and the driving device is started to drive the rotating shaft 8 and the rotating table 11 to rotate, and the mounting groove 16 is rotated to the bottom of the through groove 7. The lifting platform 12 and the culture dish 9 are driven to rise by the electric push rod 17 until the culture dish 9 passes through the through groove 7. At this time, the culture dish 9 can be taken out. During the process of taking out, the whole process is mechanically controlled. Compared with manual taking out, the stability is higher, and there is no contact with other culture dishes 9 during the taking out process, which is safer and faster.

[0029] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A primary lung cancer cell separation and culture device, comprising an incubator (1), characterized in that: A heating plate (2) is installed at the lower end of the incubator (1), a cover (4) is installed at the upper end of the incubator (1), a through slot (7) is provided at the upper end of the cover (4), an opening and closing door (5) is rotatably connected to the surface of the through slot (7), a rotating shaft (8) is rotatably connected to the center position of the interior of the incubator (1), a driving device is connected to the lower end of the rotating shaft (8) located at a position inside the incubator (1), a rotating table (11) is fixedly installed on the surface of the rotating shaft (8), and the rotating table (11) is The surface array is provided with a plurality of mounting grooves (16), an open groove is provided at the bottom center of the mounting groove (16), an electric push rod (17) is installed inside the open groove, a push plate (18) is installed at the upper end of the electric push rod (17), a lifting platform (12) is installed at the upper end of the push plate (18), the lifting platform (12) is slidably connected to the inside of the mounting groove (16), a culture dish (9) is placed at the upper end of the lifting platform (12), and the size of the culture dish (9) is smaller than the size of the through groove (7).

2. The primary lung cancer cell isolation and culture device according to claim 1, characterized in that: A control platform (3) is installed at the front end of the incubator (1), a display screen (19) is provided on the surface of the control platform (3), and multiple groups of control components (10) are installed on the surface of the control platform (3) below the display screen (19), and each of the control components (10) is electrically connected to an electric push rod (17).

3. The primary lung cancer cell isolation and culture device according to claim 2, characterized in that: The control assembly (10) comprises an ascending control button (1001) and a descending control button (1002), and both the ascending control button (1001) and the descending control button (1002) are electrically connected to the electric push rod (17).

4. The device for isolating and culturing primary lung cancer cells according to claim 3, wherein: A temperature sensor is installed inside the installation groove (16), and a plurality of the temperature sensors are electrically connected to the display screen (19).

5. The primary lung cancer cell isolation and culture device according to claim 1, characterized in that: A plurality of grooves (14) are arranged in an array on the side wall surface of the installation slot (16), and a guide rod (15) is installed inside the groove (14). The plurality of guide rods (15) are fixedly connected to the lifting platform (12) together, and the upper end of the guide rod (15) passes through the groove (14) through a hole and extends to the top of the rotating table (11).

6. The primary lung cancer cell separation and culture device according to claim 1, characterized in that: The bottom array of the incubator (1) is provided with a plurality of air vents (13), the air vents (13) being located between the rotating platform (11) and the side wall of the incubator (1), and the side wall surface of the cover (4) is provided with a plurality of ventilation holes (6).

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