Tissue immunofluorescence bleaching and dyeing equipment

Abstract

The utility model discloses tissue immunofluorescence bleaching and dyeing equipment, which relates to the technical field of immunofluorescence and comprises a bottom plate, a cover plate is arranged on the upper side of the bottom plate, a groove is arranged on the upper side surface of the cover plate, the bottom end of the cover plate penetrates into the groove and is provided with a drain hole, and a water blocking plug is arranged on the upper side surface of the bottom plate and penetrates through the drain hole. According to the utility model, waste of tissues during slicing is avoided, a plurality of samples can be treated at the same time, the tissues can be dyed more fully and thoroughly, and non-specific dyeing can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of immunofluorescence, in particular to a tissue immunofluorescence bleaching and dyeing device. Background Art

[0002] Immunofluorescence technology is a powerful tool for detecting and locating specific proteins or antigens. It is widely used in biomedical research and clinical diagnosis. Its principle is to use specific antibodies to bind to target antigens. Antibodies can be labeled with fluorescent dyes such as FITC and APC. When these labeled antibodies bind to antigens, they will emit fluorescence of a specific wavelength when irradiated with light. The sample can be observed using a fluorescence microscope to capture the emitted fluorescence for qualitative or quantitative analysis of the location and expression level of the target antigen. The steps of tissue immunofluorescence include: sample fixation and embedding, sectioning and mounting, washing and circling, sample permeabilization, blocking to reduce non-specific binding, incubation with primary antibody and fluorescent-labeled secondary antibody, rinsing, and finally imaging and analysis of the results using a fluorescence microscope.

[0003] The defects and deficiencies of the existing technology include poor stability: when performing conventional tissue immunofluorescence, the tissue needs to be embedded and sliced, and then mounted on a glass slide. Subsequently, the OCT or paraffin is removed and a water barrier is drawn. However, because the water barrier is drawn manually, it is easy to cause the water barrier to be of different sizes and at different distances from the tissue, resulting in uneven staining of the same tissue and different fluorescence intensities of different tissues; incomplete staining: when using a glass slide for immunofluorescence, only the exposed surface can be fully in contact with the punching solution, blocking solution, antibody, etc., while the other side is in contact with the glass slide. When the tissue section is thick, it often leads to a darker background. There is a lot of non-specific staining; the problem of slide falling: when adhesive slides are not used, or the tissue thickness is thick, or the baking is insufficient, or some tissue patches (such as skin tissue) are not firm, slides often fall when using slides for immunofluorescence, resulting in missing results, unusable results and other problems; for bleaching and staining, there is currently no fixed recommended method, and few research groups have adopted this method. The equipment used by each laboratory is different. Therefore, we hope to have a device that can assist in the tissue immunofluorescence bleaching and staining steps to solve the above defects and shortcomings of immunofluorescence and make tissue immunofluorescence staining more uniform and stable. Utility Model Content

[0004] Based on this, the purpose of the present invention is to provide a tissue immunofluorescence bleaching device to solve the above technical problems.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a tissue immunofluorescence bleaching device, comprising a base plate, characterized in that: a cover plate is provided on the upper side of the base plate, and a groove is provided on the side surface of the cover plate, the bottom end of the cover plate passes through to the inside of the groove and a drainage hole is provided, a water blocking plug is provided on the side surface of the base plate, and the water blocking plug passes through the drainage hole.

[0006] By adopting the above technical solution: when in use, the cover plate is fitted with the bottom plate, and the water blocking plug is inserted into the drainage hole. The drainage hole is blocked, and tissue slices and corresponding solutions can be placed in the groove. After a certain period of treatment, the bottom plate is removed, and an absorbent paper can be placed under the groove to promote the clean flow of liquid. Then the bottom plate and the cover plate are fitted together and new liquid is added.

[0007] The present invention is further configured such that the size of the bottom plate is the same as the size of the cover plate.

[0008] By adopting the above technical solution, the tissue section processing effect is improved.

[0009] The present invention is further configured such that the water blocking plugs are provided in multiple groups, and each group of water blocking plugs is provided with six water blocking plugs, and the diameter of the water blocking plugs is the same as the inner diameter of the drainage hole.

[0010] By adopting the above technical solution, the water blocking plug is inserted into the drainage hole, so that the sealing performance of the interior of the groove is better.

[0011] The present invention is further configured such that the grooves are provided in a plurality of groups, and the heights of the drainage holes on the upper side surfaces inside the grooves are the same.

[0012] By adopting the above technical solution, the tissue slices placed in the groove can be better and more comprehensively soaked and dyed.

[0013] In summary, the present invention has the following beneficial effects:

[0014] The utility model does not cause waste of tissue during sectioning, can process multiple samples at the same time, can make tissue staining more sufficient and thorough, and can reduce non-specific staining. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the top view of the structure of the utility model;

[0016] Figure 2 This is a front view structural diagram of the utility model;

[0017] Figure 3 This is a schematic diagram of the three-dimensional structure of the bottom plate of the utility model;

[0018] Figure 4 This is a schematic diagram of the top view of the cover plate of the present invention;

[0019] Figure 5 This is a schematic diagram of the front cross-sectional structure of the cover plate of the present invention.

[0020] In the figure: 1. Base plate; 2. Water blocking plug; 3. Cover plate; 4. Groove; 5. Drain hole. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0022] The following describes an embodiment of the present invention based on its overall structure.

[0023] A tissue immunofluorescence staining device, such as Figure 1 and 5 As shown, it includes a base plate 1, a cover plate 3 is provided on the upper side of the base plate 1, and a groove 4 is provided on the upper side of the cover plate 3, the bottom end of the cover plate 3 passes through the groove 4 and a drainage hole 5 is provided inside, a water blocking plug 2 is provided on the upper side of the base plate 1, and the water blocking plug 2 passes through the drainage hole 5. When in use, the cover plate 3 is fitted with the base plate 1, and the water blocking plug 2 is inserted into the drainage hole 5, and the drainage hole 5 is blocked. Tissue slices and corresponding solutions including PBS, permeabilization liquid, blocking liquid, antibodies, DAPI, etc. can be placed in the groove 4. After a certain period of treatment, the base plate 1 is removed, and an absorbent paper can be placed under the groove 4 to promote the clean flow of the liquid. Then the base plate 1 is fitted with the cover plate 3 and new liquid is added.

[0024] See also Figure 2 The size of the bottom plate 1 is the same as that of the cover plate 3, which makes the tissue section processing effect better.

[0025] See also Figure 1 and Figure 3 There are multiple groups of water blocking plugs 2, and each group of water blocking plugs 2 is provided with six. The diameter of the water blocking plug 2 is the same as the inner diameter of the drainage hole 5, so that the water blocking plug 2 is inserted into the drainage hole 5, making the internal sealing of the groove 4 better.

[0026] See also Figure 1 、 Figure 4 and Figure 5 There are multiple groups of grooves 4, and the height of the drainage holes 5 on the upper side of the grooves 4 is the same; so that the tissue slices placed in the grooves 4 can be better and more comprehensively soaked and dyed.

[0027] The working principle of the present invention is as follows: when in use, the cover plate 3 is fitted with the bottom plate 1, and the water blocking plug 2 is inserted into the drainage hole 5. The drainage hole 5 is blocked, and tissue sections and corresponding solutions including PBS, permeabilization solution, blocking solution, antibodies, DAPI, etc. can be placed in the groove 4. After a certain period of treatment, the bottom plate 1 is removed, and an absorbent paper can be placed under the groove 4 to promote the clean outflow of the liquid. Then, the bottom plate 1 and the cover plate 3 are fitted together and new liquid is added.

[0028] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not limitations on the present invention. The specific features, structures, materials, or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and purpose of the present invention, but as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A tissue immunofluorescence dyeing device, comprising a bottom plate (1), characterized in that: A cover plate (3) is provided on the upper side of the bottom plate (1), and a groove (4) is provided on the upper side of the cover plate (3); a drainage hole (5) is provided at the bottom end of the cover plate (3) extending through the groove (4); a water blocking plug (2) is provided on the upper side of the bottom plate (1), and the water blocking plug (2) extends through the drainage hole (5).

2. The tissue immunofluorescence dyeing device according to claim 1, characterized in that: The size of the bottom plate (1) is the same as the size of the cover plate (3).

3. The tissue immunofluorescence dyeing device according to claim 1, characterized in that: The water blocking plugs (2) are provided in multiple groups, and each group of water blocking plugs (2) is provided with six water blocking plugs. The diameter of the water blocking plugs (2) is the same as the inner diameter of the drainage hole (5).

4. The tissue immunofluorescence dyeing device according to claim 1, characterized in that: The grooves (4) are provided in multiple groups, and the drainage holes (5) on the upper side surfaces inside the grooves (4) have the same height.

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