Digestion and dyeing device for peripheral blood chromosome G banding

Abstract

The utility model discloses a digestion and dyeing device for peripheral blood chromosome G banding. The digestion and dyeing device comprises a bracket, an upper box body, a water tank and a built-in carrier, the upper box body is arranged at the upper part of the bracket and comprises three kits which are arranged side by side in the horizontal direction and a heating part for heating the kits; the three kits are a first kit, a second kit and a third kit in the same direction in sequence; at least one water tank is arranged at the lower part of the bracket; the built-in carrier frame is detachably erected on the kit or the water tank, and a plurality of inserting components for detachably inserting slides are arranged on the built-in carrier frame. According to the utility model, the aim of batch destaining is fulfilled, the time and reagents required by experiments are saved, and the quality and efficiency are improved; the distances among the digestive juice container, the NaCl solution container and the dye solution container are eliminated, and the water surface of the incubation part is completely covered, so that the condition that the experiment fails due to the fact that the slide falls into the water in the transfer process is avoided; and the condition of experiment failure caused by the blockage of the slide is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiments, in particular to a digestion and staining device for peripheral blood chromosome G banding. Background Art

[0002] G-banding of peripheral blood lymphocyte chromosomes is widely used in clinical practice. As one of the most fundamental and effective methods in genetic diagnosis, it has been widely applied in cytogenetic research and the diagnosis of chromosomal diseases. Because this technique requires long cell culture times and a complex preparation process, it is susceptible to factors such as temperature, humidity, pH, hemolysis, and coagulation. Therefore, carefully controlling each experimental step is crucial to the success of the experiment.

[0003] At present, the existing digestion-staining process is as follows: in a 37°C water bath, a first vertical dyeing tank A filled with prepared trypsin is prepared, and after digestion, the slide is rinsed in a beaker B filled with PBS, and then stained in a second vertical dyeing tank C filled with the prepared Giemsa dye solution. During the operation, long tweezers are used to pick up the slide. The following four situations are prone to occur during the picking process: ① At the end of the digestion timer, the long tweezers often fail to pick up the slide, and the slide slips into the first vertical dyeing tank A of trypsin, resulting in a long digestion time and failure to produce the slide; ② The undigested slide is successfully picked up by the long tweezers, but falls into the water bath before reaching the beaker B of PBS, which not only causes failure to produce the slide, but also causes water bath contamination; ③ After digestion and rinsing, the slide slips into the water bath during the process of being picked up and transferred to the second vertical dyeing tank C with Gimsa dye solution, affecting the slide production effect and easily causing slide production failure; ④ After staining is completed, the slide slips into the water bath during the process of being taken out of the water bath, affecting the staining effect and easily causing slide production failure.

[0004] In addition, trypsin digestion and staining are both carried out in a vertical dyeing vat with a card slot. The existing vertical dyeing vat has the following disadvantages: (1) When using long tweezers to pick up single-head frosted glass slides into and out of the vat, the slides are often easily stuck or even blocked in the card slot due to the manufacturer and size deviation of each batch of glass slides, causing serious digestion and staining timeout and failure to output the slides; (2) After the test slides determine the appropriate digestion and banding time, batch digestion and staining can be carried out, but batch digestion and staining cannot be carried out using long tweezers and vertical dyeing vats. At most, two back-to-back glass slides can be clamped at a time, which reduces work efficiency. Efficiency; (3) When digesting and staining single-head frosted glass slides in a vertical dyeing tank, the slides must be placed upright and completely immersed in the pancreatic enzyme digestion solution and dye solution except for the frosted area. Therefore, the amount of digestion solution and dye solution prepared must fill a 60ml vertical dyeing tank. If the operation is not careful, it is easy to splash the pancreatic enzyme digestion solution and dye solution into the water bath, causing contamination; (4) Since batch digestion and staining cannot be performed, when a large number of slides are produced, in order to avoid the influence of the long preparation time of pancreatic enzyme digestion solution and Gimsa dye solution on the quality of the slides, new pancreatic enzyme digestion solution and dye solution must be replaced once or multiple times in the middle.

[0005] Since trypsin digestion and banding typically take around 10 seconds, staining time also has a crucial impact on the success of slide production. Even a second more or less can significantly impact the banding quality. Therefore, using existing digestion and staining equipment, routine work often involves smooth initial harvesting and slide preparation, but errors in banding (trypsin digestion) and staining can occur. These errors often negate all previous efforts, often resulting in slide failures such as no banding or blurred banding. Furthermore, after digestion and staining, a separate cleaning device is required to remove excess dye, and there is a certain delay in removing the slides from the cleaning frame. Utility Model Content

[0006] The utility model aims to provide a digestion and staining device for peripheral blood chromosome G banding, so as to solve the problems of easy mistakes and inability to digest and stain in batches in existing digestion and staining devices.

[0007] To solve the above problems, the present invention is achieved as follows:

[0008] A digestion and staining device for peripheral blood chromosome G banding, comprising: a support, an upper box, a water tank, and a built-in carrier;

[0009] The upper box is arranged on the upper part of the bracket, and the upper box includes three reagent kits arranged side by side in a horizontal direction, and a heating unit for heating the three reagent kits; the three reagent kits are arranged in the same direction in order: a first reagent kit for containing digestive fluid, a second reagent kit for containing NaCl, and a third reagent kit for containing dye solution;

[0010] The water tank is arranged on the lower part of the bracket in a detachable manner or a fixedly connected manner, and the number of the water tank is at least one;

[0011] The built-in carrier can be detachably mounted on the reagent box or the water tank, and a plurality of plug-in components for detachably inserting glass slides are provided on the built-in carrier.

[0012] There are two water tanks, which are arranged side by side. The arrangement direction of the two water tanks is consistent with the arrangement direction of the three test kits.

[0013] Wherein, the water tank is placed at the lower part of the bracket in a detachable manner.

[0014] Wherein, the digestion and dyeing device also includes a nozzle assembly for flushing the built-in carrier placed in the water tank.

[0015] The heating part includes a jacket and an electric heating tube arranged in the jacket. The jacket is filled with a heating agent. The jacket is connected to a liquid inlet and a liquid outlet.

[0016] The number of the electric heating tubes is consistent with the number of the reagent kits and corresponds one to one, and the electric heating tubes are located below the corresponding reagent kits.

[0017] Among them, the built-in carrier includes a main frame, a handle plate, and a lap plate; the main frame is a rectangular box structure with an open top, and the handle plate and the lap plate are respectively arranged on two opposite sides of the main frame; multiple plug-in components are arranged in multiple rows, and the plug-in components in two adjacent rows are staggered.

[0018] Each of the plug-in components includes two strip-shaped sockets, which are respectively provided on two opposite sides of the main frame and are arranged opposite to each other.

[0019] Wherein, a limiting platform is provided below one of the strip-shaped jacks, and the limiting platform is fixedly provided on the inner side surface of the main frame.

[0020] Wherein, a drainage hole is provided on the side or bottom of the main frame.

[0021] The beneficial effects of the utility model are:

[0022] During use, the present invention heats the test kit to 37°C via the heating unit. After the temperature stabilizes, a pancreatic enzyme digestion reagent is prepared in the first test kit, a NaCl reagent is prepared in the second test kit, and a dye reagent is prepared in the third test kit. Pure water is then added to the water tank. Multiple slides are inserted into the plug-in assembly of the built-in carrier. During dyeing, the built-in carrier containing the slides is first placed in the first test kit. After the timer expires, the built-in carrier is immediately removed and placed in the second test kit to terminate the digestion. The carrier is then placed in the third test kit for timed dyeing. Finally, the slides are cleaned in the water tank to remove excess dye from the slides. A built-in carrier can be placed above the water tank to drain the water. The number of water tanks can be one or more. If there are multiple water tanks, they can be used alternately to increase the cleaning effect.

[0023] Compared with the traditional vertical dyeing vat, this utility model has the following advantages:

[0024] (1) The goal of batch digestion and staining was achieved, which saved the time and reagents required for the experiment and improved the quality and efficiency. The distance between the three containers of digestion solution, NaCl solution and staining solution was eliminated, and the water surface of the incubation part was completely covered, which avoided the failure of the experiment due to the slide falling into the water during the transfer process.

[0025] (2) It avoids the situation where the experiment fails due to the jamming of the slide. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The present invention will be further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0027] Figure 1 This is a schematic diagram of the structure of the utility model without the built-in carrier;

[0028] Figure 2 It is a side view of the utility model;

[0029] Figure 3 It is a structural diagram of the built-in carrier;

[0030] Figure 4 This is a schematic diagram of the internal structure of the built-in carrier.

[0031] Description of Reference Numerals

[0032] A. First vertical dye vat; B. Beaker; C. Second vertical dye vat; 1. Bracket; 2. Upper box; 3. Water tank; 4. Built-in carrier; 41. Main frame; 42. Hand grip plate; 43. Lap plate; 44. Drain hole; 5. Reagent box; 51. First reagent box; 52. Second reagent box; 53. Third reagent box; 6. Plug-in assembly; 61. Strip jack; 62. Limiting platform; 7. Nozzle assembly; 71. Connecting pipe; 72 Nozzle; 8. Heating part; 81. Jacket; 82. Electric heating tube; 83. Heating agent. DETAILED DESCRIPTION

[0033] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can also be implemented in other ways different from those described herein, and those skilled in the art can make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] like Figure 1-Figure 2As shown, the present invention is a digestion and staining device for peripheral blood chromosome G banding, comprising: a bracket 1, an upper box 2, a water tank 3, and a built-in carrier 4. The upper box 2 is arranged on the upper part of the bracket 1, and the upper box 2 includes three reagent kits 5 arranged side by side in a horizontal direction, and a heating part 8 for heating the three reagent kits 5. The reagent kits 5 are heated by the heating part 8 to keep the temperature of the reagent kits 5 at 37°. The three reagent kits 5 are arranged in the same direction in order: a first reagent kit 51 for containing digestive fluid, a second reagent kit 52 for containing NaCl, and a third reagent kit 53 for containing dye solution. Before use, the first reagent kit 51 is filled with pancreatic enzyme digestion fluid, the second reagent kit 52 is filled with NaCl solution, and the third reagent kit 53 is filled with dye solution. The water tank 3 is arranged at the lower part of the bracket 1 in a detachable manner or a fixedly connected manner, and the number of water tanks 3 is at least one. The built-in carrier 4 can be detachably mounted on the reagent box 5 or the water tank 3. The liquid in the reagent box 5 can immerse the glass slide on the built-in carrier 4. The built-in carrier 4 is provided with a plurality of plug-in components 6 for detachably inserting the glass slide. The number of plug-in components 6 is multiple. The reagent box 5 is a container structure with an open top.

[0035] like Figure 1 As shown, during use, the present invention heats the test kit 5 to 37°C via the heating unit 8. After the temperature stabilizes, a trypsin digestion reagent is prepared in the first test kit 51, a NaCl reagent is prepared in the second test kit 52, and a dye reagent is prepared in the third test kit 53. Pure water is then added to the water tank 3. Multiple slides are inserted into the plug-in assembly 6 of the built-in carrier 4. During staining, the built-in carrier 4 containing the slides is first placed in the first test kit 51. After the timed staining is completed, the built-in carrier 4 is immediately removed and placed in the second test kit 52 to terminate the staining. The slides are then placed in the third test kit 53 for a scheduled staining. Finally, the slides are washed in the water tank 3 to remove excess dye from the slides. A built-in carrier 4 can be placed above the water tank 3 to drain the water. The number of water tanks 3 can be one or more. Multiple water tanks 3 can be used alternately to enhance the cleaning effect.

[0036] like Figure 1 As shown, in this embodiment, the number of water tanks 3 is two, and the two water tanks 3 are arranged side by side. The arrangement direction of the two water tanks 3 is consistent with the arrangement direction of the three reagent kits 5. The two water tanks 3 can be used alternately and the water can be changed at any time.

[0037] like Figure 2As shown, this embodiment also includes a nozzle assembly 7, which can rinse the glass slides on the built-in carrier 4 placed in the water tank 3, and cooperate with the water in the water tank 3 to increase the rinsing effect. The nozzle assembly 7 includes a connecting pipe 71, and one or more nozzles 72 connected to the connecting pipe 71. The water source connected to the nozzle assembly 7 is pure water from the laboratory. In this embodiment, a gap is set between the top of the water tank 3 and the bottom of the upper box body 2 to facilitate the placement of the built-in carrier 4 on the water tank 3. In addition, in this embodiment, the width direction of the built-in carrier 4 is parallel to the length direction of the water tank 3, and the length of the water tank 3 is greater than twice the width of the built-in carrier 4. The nozzle assembly 7 is located on one side of the length direction of the water tank 3, so that the built-in carrier 4 can enter from the side above the upper box body 2 and then move to the bottom of the nozzle assembly 7 without interference during use.

[0038] The water tank 3 is detachably or fixedly mounted on the lower portion of the support 1. In this embodiment, the water tank 3 is detachably mounted on the lower portion of the support 1. This allows the water tank 3 to be removed at any time after rinsing the slides for subsequent processing, increasing convenience. In this embodiment, the multiple water tanks 3 are made of corrosion-resistant PP plastic.

[0039] like Figure 1-Figure 2As shown, in this embodiment, the heating unit 8 includes a jacket 81 and an electric heating tube 82 arranged in the jacket 81. The jacket 81 is filled with a heating agent 83, which is an aqueous solution. The jacket 81 is connected to a liquid inlet and a liquid outlet. In this embodiment, the jacket 81 is a closed cavity structure. The upper part of the jacket 81 is recessed with at least one groove with an upper opening. The number of grooves is consistent with the number of reagent kits 5 and corresponds one-to-one. The groove is used to place the reagent kits 5. In order to heat multiple reagent kits 5 evenly, the number of electric heating tubes 82 is consistent with the number of reagent kits and corresponds one-to-one. The electric heating tubes 82 are located below the corresponding reagent kits. In this embodiment, in order to evenly heat the reagents in the reagent kits 5, the liquid level of the heating agent 83 in the jacket 81 is higher than the liquid level of the reagents in the reagent kits 5; in order to fully treat the glass slides in the built-in carrier 4 with the reagents, when the built-in carrier 4 is placed in the corresponding reagent kit 5, the liquid level of the reagent in the reagent kit 5 is higher than the top of the top glass slide in the built-in carrier 4. The outer peripheral side of the jacket 81 is covered with an insulation layer. Firstly, the jacket 81 can be insulated to reduce power consumption. After heating to the required temperature, the electric heating tube can be turned off to maintain the temperature of the jacket for a longer time. Secondly, the operator can be protected from burns. In addition, in order to facilitate accurate adjustment of the temperature inside the jacket 81, a temperature sensor is provided in the jacket 81, and heating can be stopped after reaching the set temperature. In order to understand the liquid level in the jacket 81, a liquid level sensor is provided in the upper part of the jacket 81. An overflow pipe is also connected to the jacket 81. The bottom end of the overflow pipe extends to the outside of the jacket 81, and the top end extends to the top of the jacket 81. When the heating agent 83 is full, it will overflow to avoid excessive internal pressure and damage to the jacket. A stainless steel plate is fitted on the outside of the insulation layer to improve the overall strength.

[0040] like Figure 2-Figure 4 As shown, in this embodiment, the built-in carrier 4 includes a main frame 41, a hand grip plate 42, and a connecting plate 43. The main frame 41 is a rectangular box structure with an open top. The hand grip plate 42 and the connecting plate 43 are respectively arranged on opposite sides of the main frame 41. When in use, the hand grip plate 42 and the connecting plate 43 are respectively connected to the top of the reagent box 5 or the water tank 3 on opposite sides, so that the built-in carrier 4 is placed on the reagent box 5 or the water tank 3 through the hand grip plate 42 and the connecting plate 43, which facilitates the removal of the built-in carrier 4. In this embodiment, the hand grip plate 42 and the connecting plate 43 are fixedly arranged on the upper part of the main frame 41, and are preferably arranged at the top of the main frame 41. This ensures that the built-in carrier 4 can be completely placed in the reagent box 5 or the water tank 3 and is wrapped in the jacket 81. The slides on the built-in carrier 4 can be quickly and evenly processed accordingly, and meet the experimental requirements. In order to facilitate the connection between the reagent box 5 and the water tank 3 and the corresponding grooves or brackets, the edges of the tops of the reagent box 5 and the water tank 3 are provided with clamping edges for easy disassembly and cleaning. The built-in carrier 4 is made of PP plastic material and is corrosion-resistant.

[0041] like Figure 3-Figure 4 As shown, in this embodiment, multiple plug-in assemblies 6 are arranged in multiple rows, each row including one or more plug-in assemblies, with adjacent rows of plug-in assemblies 6 arranged in a staggered manner. Providing multiple plug-in assemblies 6 allows for batch processing of multiple slides, and the staggered arrangement of plug-in assemblies 6 in adjacent rows ensures that the slides are fully exposed to the reagents in the reagent kit 5. Each plug-in assembly 6 includes two strip-shaped receptacles 61, which are located on opposite sides of the main frame 41 and are arranged in a manner such that a slide can be clamped between the two strip-shaped receptacles 61. Furthermore, a stopper 62 can be provided below one of the strip-shaped insertion holes 61. The stopper 62 is fixedly provided on the inner side surface of the main frame 41, and the top surface of the stopper 62 is slightly lower than the bottom end of the corresponding strip-shaped insertion hole 61. With this design, when a glass slide passes through the opposite strip-shaped insertion hole 61 and reaches the strip-shaped insertion hole 61, it can be placed on the stopper 62 with a slight downward tilt. Since the top surface of the stopper 62 is slightly lower than the bottom end of the corresponding strip-shaped insertion hole 61, this side can also support the glass slide, preventing it from falling during the process of convenient glass slide insertion. In this embodiment, after the glass slide passes through one of the strip-shaped insertion holes 61, it can be inserted into the strip-shaped insertion hole 61 on the opposite side, or it can be placed on the stopper 62 on the opposite side. After cleaning, in order to facilitate the draining of the glass slide, a drain hole 44 is provided on the side or bottom of the main frame 41. The horizontal axial arrangement of the strip-shaped insertion hole 61 enables the slide to be placed horizontally in the built-in carrier, and enables batch digestion and staining, thereby reducing the usage of trypsin digestion solution and Gimsa staining solution and saving reagents.

[0042] In the present invention, the built-in carrier 4 has the following advantages:

[0043] (1) Batch digestion and staining can be achieved;

[0044] (2) The built-in carrier 4 can completely prevent the slide from slipping into the pancreatic digestion solution due to failure in gripping, resulting in excessive digestion time and failure to remove the slide;

[0045] (3) The built-in carrier 4 can completely avoid the situation in which the slide falls into the water bath during the gripping process when performing the G-banding technique in the prior art device, resulting in failure to eject the slide and contamination of the water bath;

[0046] (4) The built-in carrier 4 can realize batch digestion and staining, which can not only improve work efficiency, but also better control the production effect, thereby achieving the purpose of improving quality and increasing efficiency;

[0047] (5) In the built-in carrier 4, since the glass slides are placed horizontally in the built-in carrier and batch digestion and staining can be achieved, the use of trypsin digestion solution and Gimsa staining solution can be reduced, saving reagents.

[0048] Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

Claims

1. A digestion and staining device for peripheral blood chromosome G banding, characterized in that: include: Bracket, upper box, water tank, built-in carrier; The upper box is arranged on the upper part of the bracket, and the upper box includes three reagent kits arranged side by side in a horizontal direction, and a heating part for heating the three reagent kits; The three test kits are arranged in the same direction: a first test kit for containing digestion solution, a second test kit for containing NaCl, and a third test kit for containing dye solution; The water tank is arranged on the lower part of the bracket in a detachable manner or a fixedly connected manner, and the number of the water tank is at least one; The built-in carrier can be detachably mounted on the reagent box or the water tank, and a plurality of plug-in components for detachably inserting glass slides are provided on the built-in carrier.

2. A digestion and staining device for peripheral blood chromosome G banding according to claim 1, characterized in that: There are two water tanks, which are arranged side by side. The arrangement direction of the two water tanks is consistent with the arrangement direction of the three test kits.

3. A digestion and staining device for peripheral blood chromosome G banding according to claim 1, characterized in that: The water tank is placed on the lower part of the bracket in a detachable manner.

4. A digestion and staining device for peripheral blood chromosome G banding according to claim 1, characterized in that: The digestion and staining device further comprises a nozzle assembly for flushing the built-in carrier placed in the water tank.

5. The digestion and staining device for peripheral blood chromosome G banding according to claim 1, characterized in that: The heating part includes a jacket and an electric heating tube arranged in the jacket. The jacket is filled with a heating agent. The jacket is connected to a liquid inlet and a liquid outlet.

6. The digestion and staining device for peripheral blood chromosome G banding according to claim 5, characterized in that: The number of the electric heating tubes is consistent with the number of the reagent kits and corresponds one to one, and the electric heating tubes are located below the corresponding reagent kits.

7. The digestion and staining device for peripheral blood chromosome G banding according to claim 1, characterized in that: The built-in carrier includes a main frame, a handle plate, and a lap plate; the main frame is a rectangular box structure with an open top, and the handle plate and the lap plate are respectively arranged on opposite sides of the main frame; the multiple plug-in components are arranged in multiple rows, and the plug-in components in two adjacent rows are staggered.

8. The digestion and staining device for peripheral blood chromosome G banding according to claim 7, characterized in that: Each of the plug-in components includes two strip-shaped plug-ins, which are respectively arranged on two opposite sides of the main frame and are arranged opposite to each other.

9. The digestion and staining device for peripheral blood chromosome G banding according to claim 8, characterized in that: A limiting platform is provided below one of the strip-shaped jacks, and the limiting platform is fixedly provided on the inner side surface of the main frame.

10. The digestion and staining device for peripheral blood chromosome G banding according to claim 7, characterized in that: A drainage hole is provided on the side or bottom of the main frame.

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