Shake-up equipment for cell culture

By designing an automated cell culture shaking device and using a rotary motor to drive the reciprocating screw and gear ring, the automatic shaking operation of the test tube is achieved, which solves the problem of time-consuming and labor-intensive manual shaking and improves efficiency and the test tube processing capacity.

CN223342678UActive Publication Date: 2025-09-16THE FIRST HOSPITAL OF LANZHOU UNIV
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Patent Information

Application Number
CN202422570360.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-10-24
Publication Date
2025-09-16
Estimated Expiration
2034-10-24

AI Technical Summary

Technical Problem

In the current cell culture process, the shaking operation mainly relies on manual labor, which leads to a large workload, time-consuming and labor-intensive process, and a limited number of shaken test tubes.

Method used

A cell culture shaking device including a top frame, a bottom plate, a shaking seat, a gear ring, a reciprocating mechanism and a clamping mechanism was designed. The reciprocating screw driven by a rotating motor drives the gear ring and the rotating rod to realize the automatic shaking operation of the test tube.

Benefits of technology

The automatic shaking process of the test tubes is realized, which reduces the workload of the staff and improves the shaking efficiency and the test tube quantity processing capacity.

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Abstract

The utility model relates to cell culture shake-up equipment which comprises a top frame and a bottom plate, two supporting plates are fixedly connected to the front face and the rear face of the top frame respectively, the lower ends of the supporting plates are fixedly connected with the upper end face of the bottom plate, a shake-up base is arranged between the top frame and the bottom plate, a plurality of containing grooves are formed in the upper end face of the shake-up base, and clamping mechanisms are arranged in the containing grooves. A gear ring is arranged in the top frame, connecting plates are arranged in front of and behind the gear ring, the connecting plates are fixedly connected with the surface of the shaking-up seat, a rotating rod which penetrates through the two connecting plates and is fixedly connected with a penetrating part is fixedly connected to the middle of the gear ring, and the rotating rod penetrates through the top frame and is rotationally connected with the penetrating part through a bearing; and a reciprocating mechanism is arranged above the gear ring. The shaking-up equipment for cell culture disclosed by the utility model can realize automatic shaking-up treatment of test tubes, reduces the working intensity of workers, can simultaneously shake up various test tubes with different diameters, and is relatively practical.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture shaking device. Background Art

[0002] Cell culture is a method of simulating the in vivo environment (sterility, suitable temperature, pH, and nutritional conditions) in vitro to allow cells to survive, grow, reproduce, and maintain their primary structure and function. Cell culture is also known as cell cloning technology, and its formal term in biology is cell culture technology.

[0003] When conducting cell culture experiments in test tubes, shaking is required. Most of the existing shaking operations are performed manually by experimenters, which increases the workload of experimenters and is time-consuming and labor-intensive. In addition, the number of test tubes that can be shaken is limited. Therefore, we propose a cell culture shaking device. Utility Model Content

[0004] The utility model provides a cell culture shaking device, which solves the technical problems raised by the background technology in the prior art.

[0005] The solution of the utility model to solve the above technical problems is as follows: it includes a top frame and a bottom plate, and the front and back of the top frame are fixedly connected to two support plates, the lower end of the support plate is fixedly connected to the upper end surface of the bottom plate, a shaking seat is provided between the top frame and the bottom plate, and a plurality of placement grooves are provided on the upper end surface of the shaking seat, and a clamping mechanism is provided inside the placement groove; a gear ring is provided inside the top frame, and connecting plates are provided at the front and back of the gear ring, and the connecting plates are fixedly connected to the surface of the shaking seat, and a rotating rod that passes through the two connecting plates and is fixedly connected to the through-part is fixedly connected in the middle of the gear ring, the rotating rod passes through the top frame and is rotatably connected to the through-part through a bearing, a reciprocating mechanism is provided above the gear ring, and a controller is fixedly connected to the left end of the top frame.

[0006] On the basis of the above technical solution, the present invention can also be improved as follows.

[0007] Furthermore, the lower end surface of the bottom plate is fixedly connected with an anti-slip pad, and the reciprocating mechanism includes two fixed plates fixedly connected to the upper end surface inside the top frame, and a reciprocating slide plate is provided between the two fixed plates.

[0008] Furthermore, the upper end surface of the reciprocating slide is fixedly connected to a limiting sliding block that is slidably connected to the inner wall of a limiting sliding groove opened on the upper end surface inside the top frame, and the lower end surface of the reciprocating slide is provided with a plurality of gear teeth, which are meshed with the rotating gear.

[0009] Furthermore, a reciprocating screw rod is provided between the two fixed plates, which passes through the reciprocating slide plate and is threadedly connected to the penetrating portion. The reciprocating screw rod passes through the two fixed plates and is rotatably connected to the penetrating portion through a bearing. A rotating motor is provided at one end of the reciprocating screw rod, whose output end is fixedly connected to one end of the reciprocating screw rod through a rotating shaft. The rotating motor is fixedly connected to the inner surface of the top frame, and the rotating motor is electrically connected to the controller.

[0010] Furthermore, the clamping mechanism includes a fixed clamping block fixedly connected to the inner surface of the placement groove, a movable clamping block is provided at one end of the fixed clamping block, and the curved surfaces of the movable clamping block and the fixed clamping block are fixedly connected to rubber pads.

[0011] Furthermore, the movable clamping block is rotatably connected to a threaded rod on one side, the threaded rod passes through the shaking seat and is threadedly connected to the passing part, one end of the threaded rod is fixedly connected to a rotating cap, and the movable clamping plate is fixedly connected to a limiting rod on one side, the limiting rod passes through the shaking seat and is slidably connected to the passing part.

[0012] The beneficial effects of the present invention are as follows: the present invention provides a cell culture shaking device, which has the following advantages:

[0013] Through the cooperation among the top frame, controller, rotating rod, gear ring, reciprocating mechanism, connecting plate, shaking seat, placement slot, clamping mechanism and support plate, the test tube can be automatically shaken, which reduces the workload of the staff and improves practicality.

[0014] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and to implement it according to the contents of the description, the following is a detailed description of the preferred embodiments of the present invention with the accompanying drawings. The specific implementation methods of the present invention are given in detail in the following embodiments and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0016] Figure 1 A schematic structural diagram of a cell culture shaking device provided in one embodiment of the present invention;

[0017] Figure 2 for Figure 1 A schematic side view of the structure of a cell culture shaking device is provided;

[0018] Figure 3 for Figure 1A schematic diagram of a partial structure of a cell culture shaking device is provided;

[0019] Figure 4 for Figure 3 A schematic side cross-sectional view of a local structure of a cell culture shaking device is provided;

[0020] Figure 5 for Figure 1 Provided is a schematic top-down cross-sectional view of the structure of a shaking seat in a cell culture shaking device.

[0021] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0022] 1. Controller; 2. Top frame; 3. Support plate; 4. Rotating rod; 5. Connecting plate; 6. Shaking seat; 7. Rotating cap; 8. Limiting rod; 9. Anti-slip pad; 10. Bottom plate; 11. Rotating motor; 12. Fixed plate; 13. Limiting slide; 14. Limiting slider; 15. Reciprocating screw; 16. Reciprocating slide; 17. Gear teeth; 18. Gear ring; 19. Placement slot; 20. Fixing clamp; 21. Rubber pad; 22. Threaded rod. DETAILED DESCRIPTION

[0023] The following is combined with Figure 1-5 The principles and features of the present invention are described, and the examples provided are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The following paragraphs describe the present invention in more detail by way of example with reference to the accompanying drawings. The advantages and features of the present invention will become more apparent from the following description and claims. It should be noted that the drawings are greatly simplified and not to exact scale, and are intended solely to facilitate and clearly illustrate the embodiments of the present invention.

[0024] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may also be a central component. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may also be a central component. When a component is considered to be "set on" another component, it may be directly set on the other component or there may also be a central component. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of this invention are intended only to describe specific embodiments and are not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0026] like Figure 1-5 As shown, the utility model provides a cell culture shaking device, including a top frame 2 and a bottom plate 10, the front and back of the top frame 2 are fixedly connected to two support plates 3, the lower end of the support plate 3 is fixedly connected to the upper end surface of the bottom plate 10, a shaking seat 6 is arranged between the top frame 2 and the bottom plate 10, the upper end surface of the shaking seat 6 is provided with a plurality of placement grooves 19, and a clamping mechanism is arranged inside the placement groove 19, a gear ring 18 is arranged inside the top frame 2, and connecting plates 5 are arranged at the front and back of the gear ring 18, and the connecting plate 5 is fixedly connected to the surface of the shaking seat 6, and a rotating rod 4 that passes through the two connecting plates 5 and is fixedly connected to the through-part is fixedly connected in the middle of the gear ring 18, the rotating rod 4 passes through the top frame 2 and is rotatably connected to the through-part through a bearing, a reciprocating mechanism is arranged above the gear ring 18, and the left end of the top frame 2 is fixedly connected to the controller 1.

[0027] Preferably, the lower end surface of the base plate 10 is fixedly connected to the anti-slip pad 9, and the reciprocating mechanism includes two fixed plates 12, both of which are fixedly connected to the upper end surface inside the top frame 2. A reciprocating slide plate 16 is arranged between the two fixed plates 12. The setting of the reciprocating slide plate 16 can make the gear ring 18 rotate back and forth under the action of the gear teeth 17.

[0028] Preferably, the upper end face of the reciprocating slide 16 is fixedly connected to a limiting slider 14 which is slidably connected to the inner wall of a limiting groove 13 provided on the upper end face of the top frame 2. The lower end face of the reciprocating slide 16 is provided with a plurality of gear teeth 17 which are meshed with the rotating gear. With the cooperation of the limiting slider 14 and the limiting groove 13, the reciprocating slide 16 can perform limited sliding.

[0029] Preferably, a reciprocating screw 15 is provided between the two fixed plates 12, which passes through the reciprocating slide plate 16 and is threadedly connected to the passing portion. The reciprocating screw 15 passes through the two fixed plates 12 and is rotatably connected to the passing portion through a bearing. One end of the reciprocating screw 15 is provided with a rotating motor 11 whose output end is fixedly connected to one end of the reciprocating screw 15 through a rotating shaft. The rotating motor 11 is fixedly connected to the inner surface of the top frame 2. The rotating motor 11 is electrically connected to the controller 1. The setting of the rotating motor 11 can drive the reciprocating screw 15 to rotate.

[0030] Preferably, the clamping mechanism includes a fixed clamping block 20 fixedly connected to the inner surface of the placement groove 19, a movable clamping block is provided at one end of the fixed clamping block 20, and the curved surfaces of the movable clamping block and the fixed clamping block 20 are fixedly connected to rubber pads 21.

[0031] Preferably, one side of the movable clamp is rotatably connected to a threaded rod 22, which passes through the shaking seat 6 and is threadedly connected to the passing portion, one end of the threaded rod 22 is fixedly connected to a rotating cap 7, and one side of the movable splint is fixedly connected to a limiting rod 8, which passes through the shaking seat 6 and is slidably connected to the passing portion. The setting of the limiting rod 8 can enable the movable clamp to perform limited sliding.

[0032] The specific working principle and usage of this utility model are as follows:

[0033] The utility model provides a cell culture shaking device. When in use, the threaded rod 22 is rotated by rotating the rotating cap 7, so that the movable clamping block is separated from the fixed clamping block 20, and then the test tube is placed between the movable clamping block and the fixed clamping block 20. Then, the rotating cap 7 is reversed to reverse the threaded rod 22, so that the movable clamping block moves toward the fixed clamping block 20, so that the test tube is limited and clamped. Since a plurality of placement grooves 19 are provided on the upper end surface of the shaking seat 6, a plurality of test tubes with different diameters can be placed at the same time for shaking operation. After the test tube is limited and clamped, the rotating motor 11 is started, and the rotating motor 11 rotates the reciprocating screw rod 15, and then the reciprocating slide plate 16 reciprocates left and right under the action of the limit slider 14 and the limit slide groove 13, and then the gear ring 18 drives the rotating rod 4 to rotate back and forth under the action of the gear teeth 17, and then the shaking seat 6 swings back and forth left and right under the action of the connecting plate 5, so that multiple test tubes can be shaken simultaneously, reducing the workload of the staff.

[0034] The above description is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Any ordinary technician in this industry can smoothly implement the present invention as shown in the drawings and described above. However, any equivalent changes, modifications and evolutions made by technicians familiar with this profession without departing from the scope of the technical solution of the present invention using the technical content disclosed above are all equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the scope of protection of the technical solution of the present invention.

Claims

1. A cell culture shaking device, comprising a top frame (2) and a bottom plate (10), characterized in that: The top frame (2) is fixedly connected to two support plates (3) at the front and back sides, and the lower end of the support plate (3) is fixedly connected to the upper end surface of the bottom plate (10). A shaking seat (6) is provided between the top frame (2) and the bottom plate (10), and a plurality of placement grooves (19) are provided on the upper end surface of the shaking seat (6). A clamping mechanism is provided inside the placement groove (19). A gear ring (18) is provided inside the top frame (2), and connecting plates (5) are provided at the front and back sides of the gear ring (18). The connecting plates (5) are fixedly connected to the surface of the shaking seat (6). A rotating rod (4) that passes through the two connecting plates (5) and is fixedly connected to the through-portion is fixedly connected at the middle of the gear ring (18). The rotating rod (4) passes through the top frame (2) and is rotatably connected to the through-portion through a bearing. A reciprocating mechanism is provided above the gear ring (18). The left end of the top frame (2) is fixedly connected to a controller (1).

2. A cell culture shaking device according to claim 1, characterized in that: The lower end surface of the bottom plate (10) is fixedly connected to an anti-slip pad (9), and the reciprocating mechanism includes two fixed plates (12) both fixedly connected to the inner upper end surface of the top frame (2), and a reciprocating slide plate (16) is provided between the two fixed plates (12).

3. A cell culture shaking device according to claim 2, characterized in that: The upper end surface of the reciprocating slide plate (16) is fixedly connected to a limiting slide block (14) which is slidably connected to the inner wall of a limiting slide groove (13) provided on the upper end surface inside the top frame (2); the lower end surface of the reciprocating slide plate (16) is provided with a plurality of gear teeth (17), which are meshed with the rotating gear.

4. A cell culture shaking device according to claim 3, characterized in that: A reciprocating screw (15) is provided between the two fixed plates (12), which passes through the reciprocating slide plate (16) and is threadedly connected to the through-portion. The reciprocating screw (15) passes through the two fixed plates (12) and is rotatably connected to the through-portion via a bearing. A rotating motor (11) is provided at one end of the reciprocating screw (15), whose output end is fixedly connected to one end of the reciprocating screw (15) via a rotating shaft. The rotating motor (11) is fixedly connected to the inner surface of the top frame (2). The rotating motor (11) is electrically connected to the controller (1).

5. The cell culture shaking device according to claim 1, characterized in that: The clamping mechanism comprises a fixed clamping block (20) fixedly connected to the inner surface of the placement groove (19); a movable clamping block is provided at one end of the fixed clamping block (20); and the curved surfaces of the movable clamping block and the fixed clamping block (20) are both fixedly connected to a rubber pad (21).

6. A cell culture shaking device according to claim 5, characterized in that: One side of the movable clamp is rotatably connected to a threaded rod (22), which passes through the shaking seat (6) and is threadedly connected to the through-portion; one end of the threaded rod (22) is fixedly connected to a rotating cap (7); one side of the movable clamp is fixedly connected to a limiting rod (8), which passes through the shaking seat (6) and is slidably connected to the through-portion.