Device for mounting piece by immunochemical staining and piece bleaching method

Abstract

The device comprises a square box, an incandescent lamp is installed on the inner side of the top of the square box, and an optical filter is arranged at the bottom of the incandescent lamp; a wet box, a storage tray and a drawer type square vessel are arranged in the square box; the top of the wet box is open, and a plurality of sets of grooves are oppositely formed in the two sides of the top of the wet box and used for containing piece racks. The storage tray is used for containing materials and instruments required by mounting; the top of the drawer-type square vessel is open, a drawing box is arranged in the drawer-type square vessel and is pulled out or pushed in from the right side of the drawer-type square vessel, and a group of grooves are oppositely formed in two sides of the top of the drawer-type square vessel. According to the device, the light filter is adopted to filter light with the same wavelength as fluorescent light, so that the influence of sunlight is avoided; by moving the drawing box instead of the slide, the stability of the brain slice attached to the slide is ensured, and the purpose of leaving the liquid level at the same time is achieved; the drying environment of the slide can be manually changed through the wet box, and the drying speed of the slide can be regulated and controlled.

Description

Technical Field

[0001] The present application relates to the technical field of immunochemical staining, and in particular to a device for mounting slides using the immunochemical staining bleaching method. Background Art

[0002] Immunochemical staining is an important technique used to study biological processes such as cell proliferation, migration, and differentiation. Its principle is based on the specificity of the antigen-antibody reaction. In the experiment, a specific antibody binds to the target antigen in the tissue section, forming an antigen-antibody complex. A chemical reaction then causes the chromogen attached to the antibody to develop color, thereby visualizing the target antigen. The general operation is as follows: first, rinse the brain slices three times in a multi-well plate to remove the OCT embedding agent during slicing, and then place the brain slices in a blocking solution for about one hour, which helps to protect the stability and integrity of the brain slices, help the antibodies better bind to the target antigens on the brain slices, and improve the sensitivity and specificity of the experiment. Then, place the brain slices in the diluted primary antibody, shake on a shaker for 5 minutes, and incubate in a 4°C refrigerator for about 16-18 hours. Then, wash the incubated brain slices three times in a multi-well plate to wash away the antibodies bound to the antigens. Then, incubate the brain slices in the diluted secondary antibody. The secondary antibody can specifically bind to the Fc region of the primary antibody, and the secondary antibody itself is fluorescently labeled, so that the expression level of the target antigen on the brain slice can be judged by the number of fluorescent spots. When the staining is completed, the brain slices need to be transferred to a slide by the bleaching method, and then prepared with a mounting medium and a cover glass to facilitate confocal fluorescence photography in the next step, so as to determine the number of fluorescent spots and thus determine the expression level of the antigen on the brain slice.

[0003] Currently, the mounting process is performed in an open environment. Because sunlight encompasses a wide range of wavelengths, prolonged exposure to sunlight can accelerate the photobleaching of fluorescent molecules, leading to a decrease in fluorescence intensity or even complete loss of fluorescence, affecting experimental data. Furthermore, when removing the slide from the liquid, the brain slice often slips off the slide, and it is easy for one portion of the slide to move out of the liquid while another remains. This results in varying drying rates during the drying process, making it difficult to accurately time the addition of mounting media. Environmental factors such as temperature, humidity, and oxygen levels can also affect the drying of the slide. Summary of the Invention

[0004] This application provides a device for mounting slides using the immunochemical staining bleaching method, aiming to address all or part of the technical problems existing in the prior art. The device employs a filter to filter out light of the same wavelength as fluorescence, thus avoiding the influence of sunlight. A movable drawer, rather than a slide, ensures the stability of the brain slice attached to the slide and simultaneously removes it from the liquid surface. A humidified chamber allows for the slide drying environment to be manually altered, regulating the drying speed.

[0005] The technical solutions provided in this application are as follows:

[0006] A device for mounting slides using an immunochemical staining bleaching method, comprising:

[0007] A square box, wherein the bottom and sides of the box are opaque and the top is transparent, an incandescent lamp is installed on the inner side of the top of the box, and a filter is provided at the bottom of the incandescent lamp;

[0008] The square box is provided with a wet box, a storage tray and a drawer-type square dish;

[0009] The wet box is designed to be open at the top, and a plurality of grooves are arranged on both sides of the top of the wet box, and the grooves are used to place the film racks;

[0010] The storage tray is used to hold materials and equipment required for mounting;

[0011] The drawer-type square dish is designed to be open at the top, with a drawer box provided inside. The drawer box is pulled out or pushed in from the right side of the drawer-type square dish, and a group of grooves are provided on both sides of the top of the drawer-type square dish.

[0012] Furthermore, the sheet rack includes a main body and support rods arranged on both sides of the main body. The main body is provided with a mounting groove, the mounting groove is used to place the slide and the cover glass, and a through hole is provided in the middle of the mounting groove.

[0013] Furthermore, the storage tray is provided with a first placement dish, a second placement dish, a slide storage box, a cover glass storage box, a rack storage box, a filter storage box, a writing brush and a pencil;

[0014] The first placement dish is used to place phosphate buffer solution;

[0015] The second placement dish is used for placing the mounting medium.

[0016] Furthermore, a mounting seat with an open bottom is provided on the top of the square box, the incandescent lamp is installed in the mounting seat, and the filter is provided at the bottom of the mounting seat.

[0017] Furthermore, a door is provided on the right side of the square box, and a door handle is provided on the door.

[0018] Furthermore, the number of grooves on the wet box is 4 groups, and the 4 groups of grooves are arranged at equal intervals.

[0019] Furthermore, when in use, the slide rack is placed in the groove of the drawer-type square dish, the drawer-type square dish is filled with phosphate buffer solution, and the slide rack is adjusted so that the main body surface is parallel to the top of the drawer-type square dish, and the slide is immersed in the phosphate buffer solution.

[0020] Furthermore, a knob is provided at one end of the main body, and the knob passes through the side wall of the installation groove from the outside to the inside. A chuck is provided at the inner end of the knob, and a slot for installing a cover glass is provided on the chuck, and the knob is connected to the main body through a threaded connection.

[0021] Compared with the prior art, the present invention has the following advantages:

[0022] 1. Use filters to filter out light with the same wavelength as the brain slice fluorescence to avoid quenching of brain slice fluorescence while making it visible to the operator for easy operation.

[0023] 2. A detachable rechargeable incandescent lamp is provided to facilitate the movement of the box, which is independent of the power supply and easy to find a suitable environment for operation.

[0024] 3. After mounting the slide, the liquid level can be lowered directly by pulling out the box, so that the brain slices can be attached to the slide, avoiding the scattering of brain slices caused by direct human movement of the slide. The brain slices can also be kept out of the water for the same amount of time, avoiding the phenomenon that one part of the brain slices on the same slide is dry and cracked while the other half is still moist.

[0025] 4. The groove design can adjust the slide angle at the appropriate time, making it easy to operate.

[0026] 5. A solution can be placed under the wet box to keep the environment moist and prevent the brain slices from drying out too quickly due to external factors. Other materials can also be placed under the wet box to adjust the drying speed of the brain slices.

[0027] 6. The slide rack is designed with a through hole to facilitate the removal of slides. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the structure of the device for mounting slides using the immunochemical staining bleaching method provided in this application;

[0029] Figure 2 A top view of the internal structure of the square box in the device for mounting slides by immunochemical staining bleaching method provided in this application;

[0030] Figure 3 This is a schematic diagram of the film rack structure in this application Figure 1 ;

[0031] Figure 4 This is a schematic diagram of the film rack structure in this application Figure 2 ;

[0032] Figure 5 This is a schematic diagram of the chuck structure in this application.

[0033] The reference numerals are as follows:

[0034] 1 - Box, 101 - Door, 102 - Handle, 2 - Incandescent Lamp, 3 - Mounting Base, 4 - Filter, 5 - Wet Chamber, 6 - Slide Rack, 601 - Main Body, 602 - Slide, 603 - Mounting Slot, 604 - Support Rod, 605 - Cover Slip, 606 - Through Hole, 607 - Knob, 608 - Chuck, 609 - Slot, 7 - Groove, 8 - Storage Tray, 9 - Drawer-Type Dish, 10 - Drawer Box, 11 - Writing Brush, 12 - Pencil, 13 - First Dish, 14 - Second Dish, 15 - Slide Storage Box, 16 - Cover Slip Storage Box, 17 - Slide Rack Storage Box, 18 - Filter Storage Box. DETAILED DESCRIPTION

[0035] To make the objectives, technical solutions, and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the embodiments described below are part of the embodiments of the present application, not all of the embodiments. Generally, the components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0036] Therefore, the detailed description of the embodiments of the present application provided below in conjunction with the accompanying drawings is intended to represent only selected embodiments of the present application and is not intended to limit the scope of protection claimed in the present application. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present application without making any creative efforts shall fall within the scope of protection claimed in the present application.

[0037] It should be understood that in the description of the embodiments of this application, the terms "first," "second," etc. are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of the technical features indicated. Therefore, features defined as "first," "second," etc. may explicitly or implicitly include one or more of the described features.

[0038] In the description of the embodiments of the present application, it should be noted that, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood in a broad sense. For example, they can be fixedly connected, detachably connected, or integrally connected; they can be mechanically connected, electrically connected, or capable of mutual communication; they can be directly connected, or indirectly connected through an intermediate medium, and can be internal communication between two elements or an interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to specific circumstances.

[0039] See Figure 1 、 Figure 2 The present application provides a device for mounting slides using the immunochemical staining bleaching method, comprising a square box 1, wherein the bottom and surrounding areas of the square box 1 are designed to be opaque, and the top is designed to be transparent. An incandescent lamp 2 is installed on the inner side of the top of the square box 1, and a filter 4 is provided at the bottom of the incandescent lamp 2.

[0040] The square box 1 is provided with a wet box 5, a storage tray 8 and a drawer-type square dish 9.

[0041] The wet box 5 is open at the top, with multiple sets of grooves 7 positioned opposite each other on either side of the top. These grooves 7 accommodate a film rack 6. The film rack 6 is placed in the grooves 7, and the angle between the main body of the film rack 6 and the top plane of the wet box 5 can be adjusted by axially rotating the film rack 6. A phosphate buffer solution can be placed underneath the wet box 5.

[0042] The storage tray 8 is used for storing materials and equipment required for mounting the film.

[0043] The drawer-type square dish 9 is designed to be open at the top, and a drawer box 10 is provided inside it. The drawer box 10 is pulled out or pushed in from the right side of the drawer-type square dish 9. A group of grooves 7 are provided on both sides of the top of the drawer-type square dish 9.

[0044] In this embodiment, the box 1 can be made of acrylic. The sides and bottom of the box 1 are black, and the top is transparent to facilitate observation of internal operations. A removable rechargeable incandescent lamp 2 can be installed. One or more filters 4 can be placed. These filters 4 can filter out light emitted by the incandescent lamp 2 that has the same wavelength as the brain slice fluorescence, preventing it from affecting the fluorescence on the brain slice, which could reduce or even eliminate its fluorescence intensity and affect experimental data. The drawer-type square dish 9 is entirely made of transparent acrylic. Two grooves 7 are provided on the top. A slide rack 6 can be placed in the groove 7. The angle between the slide rack 6 and the top plane of the drawer-type square dish 9 can be adjusted by rotating the groove 7 to accelerate the flow of liquid from the slide rack 6. A drawer-like drawer 10 is provided below the drawer-type square dish 9. When phosphate buffer solution is added to the drawer-type square dish 9, the liquid level should be submerged in the slide placed on the slide rack 6. Then, by pulling the drawer 10 to the right, the liquid level is lowered to below the slide.

[0045] like Figure 3 As shown, the slide rack 6 includes a main body 601 and support rods 604 provided on both sides of the main body 601. The main body 601 is provided with a mounting groove 603 for placing a slide 602 and a cover glass 605. A through hole 606 is provided in the middle of the mounting groove 603. The through hole 606 is for facilitating the removal of the slide 602.

[0046] Optionally, the storage tray 8 is provided with a first placement dish 13 , a second placement dish 14 , a slide storage box 15 , a cover glass storage box 16 , a rack storage box 17 , a filter storage box 18 , a writing brush 11 and a pencil 12 .

[0047] The first placement dish 13 is used to place phosphate buffer solution.

[0048] The second placement dish 14 is used for placing the mounting medium.

[0049] Optionally, a mounting base 3 with an open bottom is provided on the top of the square box 1 , the incandescent lamp 2 is installed in the mounting base 3 , and the filter 4 is provided at the bottom of the mounting base 3 .

[0050] Optionally, a door 101 is provided on the right side of the box 1 , and a door handle 102 is provided on the door 101 .

[0051] Optionally, the number of the grooves 7 on the wet box 5 is 4 groups, and the 4 groups of grooves 7 are arranged at equal intervals.

[0052] Optionally, when in use, place the slide rack 6 in the groove 7 on the drawer-type square dish 9, fill the drawer-type square dish 9 with phosphate buffer solution, adjust the slide rack 6 so that the surface of the main body 601 is parallel to the top of the drawer-type square dish 9, and ensure that the slide 602 is immersed below the liquid surface of the phosphate buffer solution.

[0053] like Figure 4 、 Figure 5 As shown, a knob 607 is provided at the left end of the main body 601, and the knob 607 passes through the side wall of the installation groove 603 from the outside to the inside. A clamp 608 is provided at the inner end of the knob 607, and a slot 609 for installing the cover glass 605 is provided on the clamp 608. The knob 607 is connected to the main body 601 through a threaded connection. By rotating the knob 607, the clamp 608 can be adjusted in direction, so that the cover glass 605 is covered on the slide 602. During use, it is necessary to pay attention to the position between the slide 602, the knob 607, and the cover glass 605 to ensure that the cover glass 605 can be installed on the chuck 608. When installing the cover glass 605, the slot 609 on the chuck 608 is in the vertical direction. When the knob 607 is rotated and the chuck 608 is rotated 90°, the cover glass 605 is just changed from vertical to horizontal and can cover the slide 602.

[0054] The device provided in this application for mounting slides by immunochemical staining bleaching method has the following operating steps:

[0055] Step 1: Place the box 1 in a dark place, select a filter 4 with the same wavelength according to the fluorescence of the brain slice, and add an appropriate amount of phosphate buffer solution to the drawer-type square dish 9 and the wet box 5.

[0056] Step 2: Take a glass slide 602, number it and write the date on it, place it on the mounting slot 603 of the slide rack 6, immerse the slide rack 6 parallel to the liquid level in the drawer-type square dish 9, and use a brush 11 to stick the brain slice on the glass slide 602.

[0057] Step 3: Slowly pull out the drawer box 10 to lower the liquid level to below the slide glass 602.

[0058] Step 4: Remove the slide holder 6 and place it on the groove 7 of the wet box 5. The slide holder 6 can be angled to allow the water droplets remaining on the slide 602 to flow down.

[0059] Step 5: After a period of time, adjust the slide rack 6 to be parallel to the top of the wet box 5, drop appropriate mounting medium on the slide 602, and place a cover glass 605 on the slide 602.

[0060] The above is merely the preferred embodiment of this application, but the scope of protection of this application is not limited thereto. Any changes or substitutions within the technical scope disclosed in this application shall be included in the scope of protection of this application. Therefore, the scope of protection of this application shall be based on the scope of protection of the claims.

Claims

1. A device for mounting slides using an immunochemical staining bleaching method, characterized in that: include: A square box, wherein the bottom and sides of the box are opaque and the top is transparent, an incandescent lamp is installed on the inner side of the top of the box, and a filter is provided at the bottom of the incandescent lamp; The square box is provided with a wet box, a storage tray and a drawer-type square dish; The wet box is designed to be open at the top, and a plurality of grooves are arranged on both sides of the top of the wet box, and the grooves are used to place the film racks; The storage tray is used to hold materials and equipment required for mounting; The drawer-type square dish is designed to be open at the top, with a drawer box provided inside. The drawer box is pulled out or pushed in from the right side of the drawer-type square dish, and a group of grooves are provided on both sides of the top of the drawer-type square dish.

2. The device for mounting slides by immunochemical staining bleaching method according to claim 1, characterized in that: The sheet rack includes a main body and support rods arranged on both sides of the main body. The main body is provided with a mounting groove for placing a slide and a cover glass, and a through hole is provided in the middle of the mounting groove.

3. The device for mounting slides by immunochemical staining and bleaching method according to claim 1, characterized in that: The storage tray is provided with a first storage dish, a second storage dish, a slide storage box, a cover glass storage box, a rack storage box, a filter storage box, a writing brush and a pencil; The first placement dish is used to place phosphate buffer solution; The second placement dish is used for placing the mounting medium.

4. The device for mounting slides by immunochemical staining and bleaching method according to claim 1, characterized in that: A mounting seat with an open bottom is provided on the top of the square box, the incandescent lamp is installed in the mounting seat, and the filter is arranged on the bottom of the mounting seat.

5. The device for mounting slides by immunochemical staining and bleaching method according to any one of claims 1 to 4, characterized in that: A door is provided on the right side of the square box, and a door handle is provided on the door.

6. The device for mounting slides by immunochemical staining and bleaching method according to claim 5, characterized in that: The number of grooves on the wet box is 4 groups, and the 4 groups of grooves are arranged at equal intervals.

7. The device for mounting slides by immunochemical staining bleaching method according to claim 2, characterized in that: When in use, the slide rack is placed in the groove of the drawer-type square dish, the drawer-type square dish is filled with phosphate buffer solution, and the slide rack is adjusted so that the main body surface is parallel to the top of the drawer-type square dish, and the slide is immersed in the phosphate buffer solution.

8. The device for mounting slides by immunochemical staining and bleaching method according to claim 7, characterized in that: A knob is provided at one end of the main body, and the knob passes through the side wall of the installation groove from the outside to the inside. A chuck is provided at the inner end of the knob, and a slot for installing a cover glass is provided on the chuck, and the knob is connected to the main body through a threaded connection.

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