Rapid screening device

Abstract

The utility model discloses a rapid screening device which comprises a heat insulation container used for containing freezing media and a screening plate hung in the heat insulation container. The screening plate comprises a screening plate body and hanging pieces arranged on the two sides of the screening plate body. The screening plate body comprises a framework, containing holes are formed in the framework, the containing holes are covered with gauze structures, and slides are placed on the gauze structures. The device can be used for quickly acquiring ice-inhibiting combination information in a high-throughput manner, and quickly screening out sample combinations with good ice-inhibiting potential. The screening plate is suspended above the freezing medium, and a plurality of groups of to-be-detected solutions are simultaneously placed on the screening plate, so that the crystallization condition of each solution is observed, the ice inhibiting agent with vitrification potential is rapidly screened out, information is provided for exploration experiments of ice inhibiting combinations, and the experiment efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the field of vitrification protective agents, in particular to a rapid screening device. Background Art

[0002] Vitrification protectants consist of permeable protectants, non-permeable protectants, and additives. While there has been a steady stream of research and development reports on non-permeable protectants and additives in recent years, the development of complex vitrification protectant formulations has stagnated. This is partly due to the fact that, even with orthogonal methods to reduce the number of experiments, traditional instrumentation (cryomicroscopy and differential scanning calorimetry) still requires one to two months to perform three concentration gradients for more than four substances. This means that it is impossible to quickly identify combinations with vitrification potential. Therefore, it is necessary to develop a screening device that can rapidly identify protectants with vitrification potential to accelerate the development of complex protectants. Utility Model Content

[0003] The purpose of the utility model is to provide a rapid screening device. By suspending a screening plate above a freezing medium, multiple groups of test solutions are placed on the screening plate at the same time to observe the crystallization of each solution, and quickly screen out ice inhibitors with vitrification potential, thereby providing information for exploratory experiments on ice inhibitor combinations and improving experimental efficiency.

[0004] To achieve the above purpose, the present invention provides the following technical solutions:

[0005] The utility model discloses a rapid screening device, comprising an insulating container for containing a freezing medium and a screening plate suspended in the insulating container; wherein the screening plate comprises a screening plate body and hanging parts arranged on both sides of the screening plate body; the screening plate body comprises a frame, an accommodating hole is opened on the frame, the accommodating hole is covered with a gauze structure, and a glass slide is placed on the gauze structure.

[0006] A further solution is that the heat-insulating container is further provided with a fixing frame located below the screening plate; a through hole matching the outer contour of the screening plate body is opened on the horizontal end surface of the fixing frame.

[0007] A further solution: there are multiple accommodating holes, which are arranged in an array on the frame.

[0008] A further solution: ventilation holes are provided on the skeleton; the ventilation holes are located between adjacent accommodating holes, and the ventilation holes are arranged in an array.

[0009] A further solution: the hanging member includes a hook and a traction rope, and the two ends of the traction rope are respectively connected to the hook and the frame.

[0010] A further solution: the heat-insulating container includes a box body and a cover body arranged on the box body.

[0011] A further solution: the upper edge of the box body is provided with a notch for accommodating the hanging piece.

[0012] A further solution: the screening plate body is a rectangular structure.

[0013] A further solution: the inner wall of the box body is provided with a height mark.

[0014] Compared with the prior art, the beneficial effects of the present invention are:

[0015] The utility model can be used to obtain ice suppression combination information quickly and at high throughput, quickly screen out sample combinations with good ice suppression potential, and improve experimental efficiency. The screening plate is provided with an array of gauze structures for placing cover glasses into which sample solutions are dropped, and air holes are evenly provided in the gaps between adjacent gauze structures to facilitate better heat transfer when the freezing medium evaporates and absorbs heat. The level of the screening plate can be ensured by placing a fixed frame in the insulated container, so that the differences between the droplet samples can be compared. The fixed frame is provided with through holes for the screening plate to pass through, so that the screening plate can be moved downward to contact the freezing medium and observe the crystallization situation. A height mark is provided on the inner wall of the insulated container to facilitate control of the amount of freezing medium placed in the container and to grasp the distance between the cover glass and the freezing medium. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the main view of the utility model;

[0017] Figure 2 This is a top view of the utility model (excluding the cover);

[0018] Figure 3 This is a top view of the fixing frame in the present utility model;

[0019] In the figure: 1-insulated container, 11-box body, 111-slot, 12-cover body, 2-screening plate, 21-screening plate body, 211-skeleton, 2111-accommodating hole, 2112-ventilation hole, 212-mesh structure, 22-hanging part, 221-hook, 222-traction rope, 3-fixing frame, 31-through hole. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0021] In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the product of the present invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as a limitation on the present invention.

[0022] See also Figure 1-3 In this embodiment, a rapid screening device includes an insulated container 1 for holding a freezing medium and a screening plate 2 suspended in the insulated container 1. The screening plate 2 includes a screening plate body 21 and hanging members 22 provided on both sides of the screening plate body 21. The screening plate body 21 includes a frame 211 having a receiving hole 2111 formed therein. The receiving hole 2111 is covered with a gauze structure 212, on which a glass slide is placed. Liquid nitrogen is used as the freezing medium. The gauze structure 212 can be made of a fiber material such as cotton yarn or polyester fiber. The frame 211 is made of a plastic material such as polystyrene. The screening plate body 21 is suspended by the hanging members 22 at a level 10-15 mm above the liquid nitrogen level. During evaporation, the liquid nitrogen absorbs heat from the insulated container 1, thereby lowering the temperature within the container. The liquid to be tested is dropped onto a coverslip and crystallizes in a low-temperature environment. The degree of crystallization can be observed and recorded visually to quickly and preliminarily screen out test solutions with ice inhibition potential. The cover glass can be picked up with tweezers and placed in the gauze structure 212 in advance.

[0023] Furthermore, the insulated container 1 is provided with a mounting bracket 3 positioned below the screening plate 2. The upper surface of the mounting bracket 3 defines a through-hole 31 that matches the contour of the screening plate body 21. The mounting bracket 3 has a horizontal upper surface. Placing the screening plate 2 on the mounting bracket 3 helps maintain the levelness of the screening plate 2, enabling more precise control of the distance between the test solution on the screening plate 2 and the liquid nitrogen level, thereby improving experimental accuracy. In this embodiment, the screening plate body 21 and the through-hole 31 are rectangular in shape, approximately the same size. The screening plate body 21 can freely pass through the through-hole 31. When the screening plate body 21 is perpendicular to the through-hole 31, the upper surface of the mounting bracket 3 stably supports the screening plate body 21. Rotating the screening plate body 21 90° allows the screening plate body 21 to pass through the mounting bracket 3 and directly contact the liquid nitrogen. It should be noted that the shape of the screening plate body 21 is not limited to a rectangle; it can also be an ellipse or other shape, as long as the screening plate body 21 can be placed on the mounting bracket 3 and rotated a certain angle to move downward.

[0024] Furthermore, there are multiple receiving holes 2111 arranged in an array on the frame 211 , and each receiving hole 2111 corresponds to a glass slide, so that multiple groups of ice-inhibiting combination solutions can be placed at the same time for comparison.

[0025] Furthermore, the frame 211 is provided with air holes 2112, which are located between adjacent receiving holes 2111 and arranged in an array. When the liquid nitrogen absorbs heat and evaporates, the air holes 2112 serve as nitrogen flow channels.

[0026] Furthermore, the hanging member 22 includes a hook 221 and a traction rope 222, and the two ends of the traction rope 222 are respectively connected to the hook 221 and the frame 211. The hook 221 and the traction rope 222 can be configured to be connected as one piece and can be simply bent from iron wire.

[0027] Furthermore, the heat-insulating container 1 comprises a box body 11 and a cover 12 covering the box body 11. A notch 111 is provided on the upper edge of the box body 11 for accommodating the hanging member 22 to prevent the hook 221 from interfering with the cover 12.

[0028] Furthermore, the inner wall of the box body 11 is provided with a height mark, and the amount of liquid nitrogen poured in can be controlled according to the mark.

[0029] Prior to use, the present invention sets up a negative control group, a blank control group, and several experimental groups. The negative control solution completely freezes at a specific liquid nitrogen level, while the blank control solution partially freezes at a specific liquid nitrogen level. The blank control solution also serves as the test base solution for the experimental groups. When the ice suppression effect is achieved, the experimental groups will have significantly fewer ice crystals than the blank control groups, or even no ice crystals can be observed.

[0030] Pour liquid nitrogen into the insulated container 1 in advance so that the liquid level is 10-15mm from the upper end surface of the fixed frame 3, then cover it with the lid 12 and let it stand for 10-15 minutes to stabilize the inside of the container. Then, use a pipette to take 1μL of the multiple experimental group solutions, negative control group solution, and blank control group solution to be tested, and drop them on the coverslip on the gauze structure 212. After adding the sample, hold the hook 221 on the screening plate and place the screening plate steadily on the through hole 31 of the fixed frame 3 so that the screening plate is perpendicular to the rectangular through hole 31. Then cover it with the lid 12 and let it stand for 10-15 minutes to stabilize the liquid sample. Finally, open the lid 12, remove the hook 221 from the upper edge of the box body 11, rotate the screening plate horizontally so that it passes through the through hole 31, and move the screening plate downward until it contacts the liquid nitrogen liquid surface. At the same time, observe, take photos, or record the crystallization of the solution sample with video.

[0031] Although this specification is described according to implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

[0032] Therefore, the above are only preferred embodiments of the present application and are not intended to limit the scope of implementation of the present application; that is, all equivalent modifications made according to the scope of the claims of the present application are within the scope of protection of the claims of the present application.

Claims

1. A rapid screening device, characterized in that: The invention comprises an insulating container (1) for containing a freezing medium, and a screening plate (2) suspended in the insulating container (1); wherein the screening plate (2) comprises a screening plate body (21) and suspension members (22) provided on both sides of the screening plate body (21); the screening plate body (21) comprises a frame (211), a receiving hole (2111) is provided on the frame (211), the receiving hole (2111) is covered with a gauze structure (212), and a glass slide is placed on the gauze structure (212).

2. The rapid screening device according to claim 1, characterized in that The heat-insulating container (1) is further provided with a fixing frame (3) located below the screening plate (2); a through hole (31) matching the outer contour of the screening plate body (21) is opened on the horizontal end surface of the fixing frame (3).

3. The rapid screening device according to claim 1, characterized in that There are a plurality of accommodating holes (2111), which are arranged in an array on the skeleton (211).

4. The rapid screening device according to claim 3, characterized in that The skeleton (211) is provided with ventilation holes (2112); the ventilation holes (2112) are located between adjacent accommodating holes (2111), and the ventilation holes (2112) are arranged in an array.

5. The rapid screening device according to claim 1, characterized in that The hanging member (22) comprises a hook (221) and a traction rope (222), and two ends of the traction rope (222) are respectively connected to the hook (221) and the frame (211).

6. The rapid screening device according to claim 1, characterized in that The heat-insulating container (1) comprises a box body (11) and a cover body (12) disposed on the box body (11).

7. The rapid screening device according to claim 6, characterized in that The upper edge of the box body (11) is provided with a notch (111) for accommodating the hanging member (22).

8. The rapid screening device according to claim 1, characterized in that The screening plate body (21) is a rectangular structure.

9. The rapid screening device according to claim 6, characterized in that The inner wall of the box body (11) is provided with a height mark.

Images