Single-cell micro-droplet generation chip

Abstract

The utility model discloses a single-cell micro-droplet generation chip, and relates to the technical field of biomedicine, the single-cell micro-droplet generation chip comprises a chip body, a sealing cover movably arranged on one side of the chip body, and a connecting mechanism installed between the chip body and the sealing cover, and a fixing hole is formed in the middle of the side, away from the chip body, of the sealing cover; the sealing cover slides away from one side of the chip body, blockages in a chip runner can be rapidly and manually taken out, so that the dredging efficiency is improved, the elastic sealing ring can seal the joint of the sealing cover and the chip body by inserting the annular groove, the sealing cover slides to the original position after dredging, the pressing rod is used for jacking and inserting the sealing cover, and the blockage in the chip runner can be rapidly and manually taken out. The sealing cover is rapidly fixed through the elastic sealing ring, the chip body can be normally used without waiting after being dredged, the elastic sealing ring can be tightly pressed on the chip body through the pressing rod under the pressure of the torsion spring, and the sealing effect between the chip body and the sealing cover can be improved.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to a single-cell micro-droplet generation chip. Background Art

[0002] Compared to single-phase microfluidic systems, droplet generation chips offer advantages such as reduced sample and reagent consumption, faster mixing, reduced cross-contamination, and easier manipulation due to their water / oil two-phase separation. Currently, uncovering heterogeneity within cell populations and exploring rare cell subpopulations are crucial for advancing personalized medicine, promoting precision medicine, and deepening our understanding of biological processes. As a key technological foundation in this research field, single-cell droplet generation chips must meet the requirements of high-sensitivity, high-throughput, and high-resolution single-cell sequencing.

[0003] The patent with announcement number CN221501122U discloses "a single-cell micro-droplet generation chip, including a chip body; the chip body is provided with at least a first phase inlet, a second phase inlet, a droplet outlet, a first axisymmetric bifurcated flow channel, a second axisymmetric bifurcated flow channel and a mixing flow channel, and the flow channels on each side of the first axisymmetric bifurcated flow channel and the second axisymmetric bifurcated flow channel are smooth flow channels; the first axisymmetric bifurcated flow channel is surrounded by the outside of the second axisymmetric bifurcated flow channel. In this solution, the two flow channels respectively connected to the two phase inlets both adopt smooth axisymmetric bifurcated flow channels, which can facilitate the stable control of the chip flow rate and help ensure the stability of droplet generation."

[0004] Regarding the above-mentioned related technologies, the inventor believes that after the flow channel in the chip is blocked, liquid needs to be injected from multiple inlet cavities to flush and clear the flow channel. However, if the blockage still remains in the flow channel after flushing, the staff will need to tear off the adhesive sealing film layer to manually remove the blockage in the flow channel, and then use glue to stick the torn sealing film layer back to its original place. However, the glue needs a certain solidification and cooling time after bonding, which will affect the normal use of the chip. In addition, the bonding process is time-consuming and inconvenient, and it is also easy to leak due to loose bonding, so it needs to be improved. Utility Model Content

[0005] In order to solve the above problems, the present application provides a single-cell micro-droplet generation chip.

[0006] The single-cell micro-droplet generation chip provided in this application adopts the following technical solution:

[0007] A single-cell microdroplet generation chip includes a chip body, a cover movably arranged on one side of the chip body, and a connecting mechanism installed between the chip body and the cover. A fixing hole is provided in the middle of the side of the cover facing away from the chip body. The connecting mechanism includes a fixing plate fixedly arranged on the outer wall of one end of the chip body, and a pressure rod rotatably arranged on one side of the fixing plate and cooperatedly inserted into the fixing hole. The cover is slidably arranged in the fixing plate. An elastic sealing ring for sealing is provided between the cover and the chip body. A torsion mechanism for pressing the pressure rod toward the fixing hole is provided on one side of the fixing plate.

[0008] By adopting the above technical solution, the blockage in the chip flow channel can be quickly manually removed by sliding the cover away from one side of the chip body, thereby improving the dredging efficiency. After dredging, the cover is slid to its original position, and the cover is quickly fixed by pressing and inserting the pressure rod on the cover, so that the chip body can be used normally after dredging without waiting.

[0009] Preferably, a flow channel is provided in the chip body, an inlet and outlet cavity communicating with the flow channel is provided on one side of the chip body, and the cover is fitted and abutted against a side of the chip body close to the flow channel.

[0010] By adopting the above technical solution, the chip body can generate single-cell micro-droplets through the flow channel, and the inlet and outlet cavities facilitate the entry and exit of different liquid media into and out of the flow channel.

[0011] Preferably, a sleeve hole is provided in the fixing plate, the sealing cover is slidably connected in the sleeve hole of the fixing plate, the elastic sealing ring is fixed on one side of the sealing cover, an annular groove is provided on the side of the chip body close to the sealing cover, one end of the elastic sealing ring is fitted and inserted in the annular groove, a push-pull handle for holding is fixed at one end of the sealing cover, an inner groove is provided on the outer side wall of the push-pull handle, and a pad that cooperates with the sealing cover is fixed at the end of the chip body facing away from the fixing plate.

[0012] By adopting the above technical solution, the elastic sealing ring can seal the connection between the cover and the chip body by inserting the annular groove. By holding the push-pull handle, the cover can be slid and pushed laterally to cover or expose the flow channel of the chip body.

[0013] Preferably, a U-shaped frame is fixedly provided on one side of the fixed plate, a rotating rod is rotatably connected inside the U-shaped frame, one end of the pressure rod is fixedly connected to the outer wall of the rotating rod, and the torsion mechanism includes two groups of torsion springs fixedly provided between the two sides of one end of the pressure rod and the inner wall of the U-shaped frame, and the end of the cover away from the push-pull handle is fixedly provided with a diagonal support rod for moving with the cover and lifting the pressure rod when clearing the chip body.

[0014] By adopting the above technical solution, the U-shaped frame can connect the rotating rod and the fixed plate, the rotating rod and the pressure rod can rotate in the U-shaped frame, and under the torsion of the torsion spring, when the staff releases the pressure rod, the pressure rod can rebound to its original position.

[0015] In summary, this application includes at least one of the following beneficial technical effects:

[0016] 1. By sliding the cover away from one side of the chip body, the blockage in the chip flow channel can be quickly manually removed, thereby improving the dredging efficiency. The elastic sealing ring can be inserted into the annular groove to seal the connection between the cover and the chip body. After dredging, the cover is slid to its original position, and the pressure rod is used to press and insert the cover to quickly fix the cover. After the chip body is dredged, it can be used normally without waiting. The pressure rod can press the elastic sealing ring tightly against the chip body under the pressure of the torsion spring, which can improve the sealing effect between the chip body and the cover.

[0017] 2. By holding the push-pull handle, the cover can be slid and pushed and pulled horizontally to cover or expose the flow channel of the chip body, which is convenient for operation. When the cover is slid during the dredging process, the diagonal support rod can be slid toward the fixed plate, so that the diagonal support rod can lift and support the pressure rod pulled away from the cover, so that the staff does not need to pull the pressure rod with their hands all the time during dredging, which is convenient for dredging and practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the overall structure of a single-cell micro-droplet generation chip according to an embodiment of the present application;

[0019] Figure 2 This is a schematic diagram of the overall internal structure of a single-cell micro-droplet generation chip according to an embodiment of the present application;

[0020] Figure 3 This is a bottom view of the overall structure of a single-cell micro-droplet generation chip according to an embodiment of the present application;

[0021] Figure 4 The embodiment of this application mainly reflects Figure 3 A magnified view of the structure in the middle;

[0022] Figure numerals: 1. chip body; 11. flow channel; 12. inlet and outlet cavity; 13. annular groove; 14. pad; 2. cover; 21. elastic sealing ring; 22. push-pull handle; 23. fixing hole; 24. diagonal support rod; 3. fixing plate; 31. sleeve hole; 4. pressure rod; 41. U-shaped frame; 42. rotating rod; 43. torsion spring. DETAILED DESCRIPTION

[0023] The following is combined with Figures 1-4 This application is described in further detail.

[0024] The embodiments of the present application disclose a single-cell micro-droplet generation chip.

[0025] A single-cell microdroplet generation chip, referring to Figure 1-3 , including a chip body 1, a cover 2 and a connecting mechanism. The cover 2 is movably arranged on one side of the chip body 1, and the cover 2 can cover and shield the internal structure of the chip body 1.

[0026] Reference Figure 1-2 A flow channel 11 is provided in the chip body 1, and an inlet and outlet cavity 12 communicating with the flow channel 11 is provided on one side of the chip body 1. The cover 2 is fitted and abutted against the side of the chip body 1 close to the flow channel 11. The chip body 1 can generate single-cell microdroplets through the flow channel 11, and the inlet and outlet cavity 12 facilitates different liquid media to enter and exit the flow channel 11. The chip body 1, the flow channel 11 and the inlet and outlet cavity 12 constitute the existing single-cell microdroplet generation chip, whose internal structure and the production principle of single-cell microdroplets have been disclosed and will not be elaborated here. The cover 2 can cover the flow channel 11 or detach from the flow channel 11 to expose the flow channel 11.

[0027] Reference Figure 1-3 A fixing hole 23 is provided in the middle of the side of the cover 2 facing away from the chip body 1. The connecting mechanism includes a fixing plate 3 fixed on the outer wall of one end of the chip body 1, and a pressure rod 4 rotatably provided on one side of the fixing plate 3 and inserted into the fixing hole 23. The cover 2 is slidably provided in the fixing plate 3, and a sleeve hole 31 is provided in the fixing plate 3. The cover 2 is slidably connected to the sleeve hole 31 of the fixing plate 3. The fixing plate 3 can support and limit the cover 2 through the sleeve hole 31, so that the cover 2 can only slide horizontally. After sliding horizontally, the cover 2 can slide and move, thereby facilitating the staff to quickly and manually remove the blockage in the chip flow channel 11.

[0028] Reference Figure 2 An elastic sealing ring 21 for sealing is provided between the cover 2 and the chip body 1. The elastic sealing ring 21 is fixed on one side of the cover 2. An annular groove 13 is provided on the side of the chip body 1 close to the cover 2. One end of the elastic sealing ring 21 is fitted into the annular groove 13. The elastic sealing ring 21 can be pressed and inserted into the corresponding annular groove 13 under its own elastic force, thereby sealing between the cover 2 and the chip body 1.

[0029] Reference Figure 1-3 A push-pull handle 22 for holding is fixed at one end of the cover 2, and an inner groove is opened on the outer side wall of the push-pull handle 22. A pad 14 that cooperates with the cover 2 is fixed at the end of the chip body 1 away from the fixed plate 3. The staff can put their fingers into the inner groove of the push-pull handle 22 to push the push-pull handle 22 and the cover 2 horizontally to cover or expose the flow channel 11 of the chip body 1, which is convenient for operation. The pad 14 cooperates with the fixed plate 3 to cushion and support the chip body 1.

[0030] Reference Figure 2-4 A torsion mechanism is provided on one side of the fixed plate 3 for pressing the pressure rod 4 toward the fixing hole 23. A U-shaped frame 41 is fixedly provided on one side of the fixed plate 3. A rotating rod 42 is rotatably connected inside the U-shaped frame 41. One end of the pressure rod 4 is fixedly connected to the outer wall of the rotating rod 42. The torsion mechanism includes two groups of torsion springs 43 fixed on both sides of one end of the pressure rod 4 and between the inner wall of the U-shaped frame 41. The U-shaped frame 41 can connect the rotating rod 42 with the fixed plate 3. The rotating rod 42 and the pressure rod 4 can rotate in the U-shaped frame 41, and under the torsion of the torsion spring 43, when the staff releases the pressure rod 4, the pressure rod 4 can rebound to its original position.

[0031] Under the pressure of the torsion spring 43 on the pressure rod 4, one end of the pressure rod 4 can be inserted into the fixing hole 23 and press against the cover 2, so that the chip body 1 and the cover 2 can be fixed. Under the pressure of the torsion spring 43, the pressure rod 4 can press the elastic sealing ring 21 tightly onto the chip body 1, which can improve the sealing effect between the chip body 1 and the cover 2.

[0032] Reference Figure 1-3 At this time, the diagonal support rod 24 can lift and support the pressure rod 4 pulled away from the cover 2, so that the staff does not need to pull the pressure rod 4 with their hands all the time during dredging, which facilitates dredging.

[0033] The implementation principle of a single-cell micro-droplet generation chip in the embodiment of the present application is as follows:

[0034] When the flow channel 11 of the chip body 1 needs to be dredged, the staff can pull one end of the pressure rod 4 away from the fixing hole 23, and then hold the push-pull handle 22 to pull the cover 2 outward to slowly separate the cover 2 from the flow channel 11. The diagonal support rod 24 will move with the cover 2 and approach the fixing plate 3. Then, the pressure rod 4 is released. The released pressure rod 4 will rotate toward the chip body 1 side under the torsion force of the torsion spring 43 and collide with the diagonal support rod 24. At this time, the diagonal support rod 24 can lift and support the pressure rod 4 pulled away from the cover 2, so that the staff does not need to pull the pressure rod 4 with their hands all the time during dredging.

[0035] Then the staff can use tools such as tweezers to quickly manually remove the obstruction in the chip flow channel 11;

[0036] After clearing, the staff holds the push-pull handle 22 to push the cover 2 toward the side of the pad 14, so that the diagonal support rod 24 is separated from the pressure rod 4, and the moved cover 2 can cover the flow channel 11 again. At the same time, under the pressure of the torsion spring 43, one end of the pressure rod 4 can be fitted into the fixing hole 23 and against the cover 2, so that the chip body 1 and the cover 2 can be fixed conveniently and quickly.

[0037] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A single-cell micro-droplet generation chip, comprising a chip body (1), a cover (2) movably arranged on one side of the chip body (1), and a connecting mechanism installed between the chip body (1) and the cover (2), characterized in that: A fixing hole (23) is provided in the middle of the side of the cover (2) facing away from the chip body (1); the connecting mechanism comprises a fixing plate (3) fixed on the outer wall of one end of the chip body (1); and a pressure rod (4) rotatably arranged on one side of the fixing plate (3) and inserted into the fixing hole (23); the cover (2) is slidably arranged in the fixing plate (3); an elastic sealing ring (21) for sealing is provided between the cover (2) and the chip body (1); and a torsion mechanism for pressing the pressure rod (4) toward the fixing hole (23) is provided on one side of the fixing plate (3).

2. A single-cell micro-droplet generation chip according to claim 1, characterized in that: A flow channel (11) is provided in the chip body (1), an inlet and outlet cavity (12) communicating with the flow channel (11) is provided on one side of the chip body (1), and the cover (2) is fitted and abutted against a side of the chip body (1) close to the flow channel (11).

3. The single-cell micro-droplet generation chip according to claim 1, characterized in that: A sleeve hole (31) is provided in the fixing plate (3), and the sealing cover (2) is slidably connected in the sleeve hole (31) of the fixing plate (3).

4. The single-cell micro-droplet generation chip according to claim 1, characterized in that: The elastic sealing ring (21) is fixedly arranged on one side of the cover (2); an annular groove (13) is provided on the side of the chip body (1) close to the cover (2); and one end of the elastic sealing ring (21) is fitted and inserted into the annular groove (13).

5. The single-cell micro-droplet generation chip according to claim 1, characterized in that: A push-pull handle (22) for holding is fixedly provided at one end of the cover (2), and an inner groove is provided on the outer side wall of the push-pull handle (22).

6. The single-cell micro-droplet generation chip according to claim 5, characterized in that: A pad (14) that cooperates with and abuts against the cover (2) is fixedly provided on one end of the chip body (1) that faces away from the fixing plate (3).

7. The single-cell micro-droplet generation chip according to claim 6, characterized in that: A U-shaped frame (41) is fixedly provided on one side of the fixed plate (3), a rotating rod (42) is rotatably connected inside the U-shaped frame (41), one end of the pressure rod (4) is fixedly connected to the outer wall of the rotating rod (42), and the torsion mechanism includes two groups of torsion springs (43) fixedly provided between the two sides of one end of the pressure rod (4) and the inner wall of the U-shaped frame (41).

8. The single-cell micro-droplet generation chip according to claim 7, characterized in that: An end of the cover (2) away from the push-pull handle (22) is fixedly provided with an oblique support rod (24) for moving with the cover (2) and supporting the pressure rod (4) when clearing the chip body (1).

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