Cell culture dish convenient for Transwell experiment

Abstract

The utility model discloses a cell culture dish convenient for Transwell experiments, which belongs to the technical field of cell culture and comprises a culture vessel, a glass slide and a vertical clamping groove plate, the vertical clamping groove plate is fixed on the inner wall of the culture vessel, and a clamping groove for inserting the glass slide is arranged at the top end of the vertical clamping groove plate; a through hole is formed in the glass slide, a porous membrane is attached and fixed to one side wall of the glass slide and covers the through hole, and the glass slide is connected with the culture vessel through a vertical clamping groove plate and divides an inner cavity of the culture vessel into two culture cavities. According to the utility model, the operation process of the traditional Transwell experiment is simplified, the porous membrane does not need to be cut and flaked, the possibility that the porous membrane is damaged is effectively reduced, and the success rate of the experiment is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture dish and an experimental method convenient for Transwell experiment. Background Art

[0002] The Transwell assay is an experimental technique that can assess both cell migration and invasion abilities. The main material of this technique is a Transwell chamber with a microporous membrane. The Transwell chamber studies cell motility by simulating the behavior of cells crossing the basement membrane and is widely used in fields such as oncology, immunology, and cell biology.

[0003] The existing Transwell chamber system consists of multiple Transwell chambers placed in a multi-well plate. Cell culture procedures are performed on each chamber in the well. The chamber, shaped like a small cup that fits within the plate, is crucially designed to contain a permeable membrane at the bottom. The basic principle of a Transwell experiment involves placing a Transwell chamber in a culture plate. The upper chamber holds the upper culture medium and cells, while the lower chamber holds the lower culture medium. The upper and lower layers are separated by a microporous membrane.

[0004] In current Transwell chambers, cells penetrate the upper chamber and remain in the lower chamber. Because of the porous membrane, cells trapped in the lower chamber after passing through the upper chamber cannot be directly observed after staining. The porous membrane often needs to be cut and mounted for observation. Because the porous membrane is small and very thin, it is not only inconvenient to handle, but also easily damaged during cutting or mounting, which can affect experimental results. Utility Model Content

[0005] In order to solve the above technical problems, the utility model provides a cell culture dish that is convenient for Transwell experiment, simplifies the operation process of traditional Transwell experiment, eliminates the need to cut and slice the porous membrane, effectively reduces the possibility of damage to the porous membrane, and improves the success rate of the experiment.

[0006] In order to achieve the above-mentioned object, the utility model provides a cell culture dish that is convenient for Transwell experiment, comprising a culture vessel, a glass slide, and a vertical slot plate, wherein the vertical slot plate is fixed to the inner wall of the culture vessel, and a slot for inserting the glass slide is provided at the top of the vertical slot plate;

[0007] The glass slide is provided with a perforation, a porous membrane is fixed to one side wall of the glass slide, and the porous membrane covers the perforation; the glass slide is connected to the culture vessel through a vertical slot plate, and the inner cavity of the culture vessel is divided into two culture cavities.

[0008] Furthermore, the vertical slot plate is a U-shaped plate body consisting of a bottom plate and two side plates integrally formed at both ends of the bottom plate.

[0009] Furthermore, the vertical slot plate is made of silicone rubber.

[0010] Furthermore, the porous membrane is a polycarbonate membrane or a polyester membrane.

[0011] Furthermore, a horizontal support is provided in the culture vessel, and the slide can be placed horizontally on the horizontal support, and the height of the horizontal support is greater than the height of the bottom plate of the vertical slot plate.

[0012] Beneficial effects of the utility model:

[0013] The utility model designs a novel glass slide structure to cooperate with a culture vessel, adheres a porous membrane to the surface of the glass slide, and inoculates cells on the porous membrane of the glass slide, which is then fixed vertically in the culture vessel, and the culture vessel is divided into two culture chambers. Induction factors are added to the culture medium of one of the culture chambers to induce cell invasion and metastasis, so that the glass slide can be directly removed for staining and observation, simplifying the operation process of the traditional Transwell experiment, eliminating the need to cut the porous membrane to make slices, effectively reducing the possibility of damage to the porous membrane, and improving the success rate of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0015] Figure 2 It is a structural schematic diagram of the present invention when the slide glass is placed vertically.

[0016] Figure 3 It is a structural schematic diagram of the glass slide in the present invention when it is placed horizontally.

[0017] In the figure: 1. culture vessel; 2. slide; 3. perforation; 4. horizontal support; 5. vertical slot plate. DETAILED DESCRIPTION

[0018] The present invention will be further described in detail below with reference to the accompanying drawings.

[0019] The utility model discloses a cell culture dish which is convenient for Transwell experiments.

[0020] Reference Figure 1 and Figure 2A cell culture dish convenient for Transwell experiment includes a culture vessel 1, a slide 2 and a vertical slot plate 5, the vertical slot plate 5 is fixed to the inner wall of the culture vessel 1, and a slot for inserting the slide 2 is provided at the top of the vertical slot plate 5; a perforation 3 is provided on the slide 2, and two perforations 3 are provided in this embodiment. A porous membrane is fixed to one side wall of the slide 2, and the porous membrane covers one end of the perforation 3. The slide 2 is connected to the culture vessel 1 through the vertical slot plate 5, and the inner cavity of the culture vessel 1 is divided into two culture cavities. The vertical slot plate 5 is a U-shaped plate body consisting of a base plate and two side plates integrally formed at both ends of the base plate. The vertical slot plate 5 is made of silicone rubber. The porous membrane is a polycarbonate film or a polyester film.

[0021] During the Transwell experiment, the cells to be tested are inoculated onto the porous membrane of the slide 2. The slide 2 with cells is then inserted into the slot using the vertical slot plate 5, dividing the inner cavity of the culture vessel 1 into two culture chambers. Different culture media are then added to the two culture chambers, and induction factors are added to one of the culture chambers to induce cell invasion and metastasis. After the metastasis experiment is completed, the slide 2 can be directly removed from the slot, and the metastatic cells can be directly stained and observed without cutting the porous membrane or making slides.

[0022] Reference Figure 3 A horizontal support 4 is provided in the culture vessel 1, and the slide 2 can be placed horizontally on the horizontal support 4. The height of the horizontal support 4 is greater than the bottom plate height of the vertical slot plate 5. When conducting the experiment, the slide 2 can be placed flat on the horizontal support 4, and then the cells to be tested can be inoculated onto the carrier slide.

[0023] The working principle of the cell culture dish of the present invention is as follows: a glass slide 2 is placed on a horizontal support 4 of a culture vessel 1, and then the cells to be tested are inoculated on the porous membrane of the glass slide 2, and then the glass slide 2 is removed and inserted into the slot of a vertical slot plate 5, so that the inner cavity of the culture vessel 1 is divided into two culture chambers, and then corresponding culture media are added to the two culture chambers, and induction factors are added to the culture chamber on the side away from the porous membrane, so that the cells penetrate the porous membrane under the induction of the induction factors to invade and metastasize.

[0024] Based on the above-mentioned ideal embodiment of the present invention, and in accordance with the above description, relevant personnel can make various changes and modifications without departing from the technical scope of the present invention. The technical scope of the present invention is not limited to the content of the specification, but must be determined according to the scope of the claims.

Claims

1. A cell culture dish for Transwell experiments, characterized by: The invention comprises a culture vessel (1), a glass slide (2) and a vertical card slot plate (5), wherein the vertical card slot plate (5) is fixed to the inner wall of the culture vessel (1), and a card slot for inserting the glass slide (2) is provided at the top of the vertical card slot plate (5); The glass slide (2) is provided with a perforation (3), a porous membrane is fixed to one side wall of the glass slide (2), and the porous membrane covers the perforation, the glass slide (2) is connected to the culture vessel (1) via a vertical slot plate (5), and the inner cavity of the culture vessel (1) is divided into two culture cavities.

2. A cell culture dish for Transwell experiment according to claim 1, characterized in that: The vertical slot plate (5) is a U-shaped plate body consisting of a bottom plate and two side plates integrally formed at both ends of the bottom plate.

3. A cell culture dish for Transwell experiment according to claim 1 or 2, characterized in that: The vertical slot plate (5) is made of silicone rubber.

4. A cell culture dish for Transwell experiment according to claim 1 or 2, characterized in that: The porous membrane is a polycarbonate membrane or a polyester membrane.

5. A cell culture dish for Transwell experiment according to claim 1 or 2, characterized in that: A horizontal support (4) is provided in the culture vessel (1), and the slide (2) can be placed horizontally on the horizontal support (4). The height of the horizontal support (4) is greater than the bottom plate height of the vertical slot plate (5).

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