Antigen detection kit

Abstract

The utility model relates to the technical field of foot and mouth disease virus detection, in particular to an antigen detection kit. Comprising a detection reagent card, an ultraviolet laser pen, a sample extracting solution and a dropper, the detection reagent card comprises a plastic card shell and a test strip, the test strip is used for detecting foot-and-mouth disease viruses in to-be-detected sample serum and is inserted into the plastic card shell, and a detection line and a quality control line for observing detection results are arranged on the test strip; the dropper is used for sucking a sample extracting solution and to-be-detected sample serum and dropping the sample extracting solution and the to-be-detected sample serum onto the test strip in the plastic clamping shell; the ultraviolet laser pen is used for irradiating a fluorescent strip which is dropped on the test strip, is subjected to chromatographic reaction on the sample extracting solution and to-be-detected sample serum and is generated at the detection line and / or the quality control line. The kit is simple in structure and easy to use, an antigen detection result is observed in an auxiliary mode through the ultraviolet laser pen, and the accuracy of judgment of the detection result can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of foot-and-mouth disease virus detection, in particular to an antigen detection kit. Background Art

[0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious zoonosis caused by the foot-and-mouth disease virus (FMDV). It poses a significant threat to the livestock industry, with many domestic animals susceptible to infection, including cattle, pigs, sheep, and camels. FMDV belongs to the genus FMDV within the family Picornaviridae. Seven serotypes are known: A, O, C, SAT1, SAT2, SAT3, and Asian. The virus is frequently transmitted to healthy livestock through direct and indirect contact, infecting them via the respiratory tract, digestive tract, and damaged skin and mucous membranes. Infection is characterized by blisters and ulcers on the oral mucosa, hooves, and udder skin. It is widespread worldwide, occurring in Africa, Asia, South America, and parts of Europe. Because it can also infect humans, it has become one of the most significant global health issues for both humans and animals. Due to its highly contagious nature, once established, the disease can infect a wide range of animals, leading to high morbidity and rapid spread. It is difficult to control and eliminate, often becoming an epidemic or even a pandemic, resulting in significant economic losses.

[0003] Currently, foot-and-mouth disease (FMD) is a mandatory animal disease for international trade and a reportable disease under OE regulations. Common methods for detecting FMD virus include nucleic acid hybridization, RT-PCR, complement fixation test (CFT), immunodiffusion precipitation test (IDPT), and immunochromatography. These methods are mostly laboratory-based and require high levels of expertise. Most of these methods are demanding, require extensive instrumentation and specialized personnel, and take a long time to perform, making them unsuitable for testing in remote areas or on-site. Developing a highly sensitive, accurate, reliable, and easy-to-use detection method is crucial for monitoring and preventing FMD virus. Therefore, a solution is urgently needed.

[0004] The above content is only used to assist in understanding the technical solution of the present invention and does not mean that the above content is the closest prior art. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide an antigen detection kit, which has a simple structure and is easy to use, and can improve the accuracy of the detection result judgment by assisting observation of the antigen detection result through an ultraviolet laser pen.

[0006] To achieve the above-mentioned purpose, the technical solution of the present invention is implemented as follows: an antigen detection kit, comprising: a detection reagent card, an ultraviolet laser pen, a sample extract and a dropper; the detection reagent card comprises a plastic card shell and a test strip, the test strip is used to detect foot-and-mouth disease virus in a sample serum to be tested and is inserted into the plastic card shell, and the test strip is provided with a detection line and a quality control line for observing the test results; the dropper is used to draw the sample extract and the sample serum to be tested and drop them onto the test strip in the plastic card shell; the ultraviolet laser pen is used to irradiate the fluorescent strips produced at the detection line and / or the quality control line by the chromatographic reaction of the sample extract and the sample serum to be tested dropped onto the test strip.

[0007] Preferably, the test strip includes a PVC base plate, a sample pad, an NC membrane, a conjugation pad and a water-absorbing pad; the PVC base plate is snap-fitted with a plastic card shell; the NC membrane is adhered to the upper surface of the middle part of the PVC base plate; the water-absorbing pad is adhered to one end of the PVC base plate and covers the NC membrane, and the water-absorbing pad overlaps the NC membrane by 2 mm; the conjugation pad is adhered to the other end of the PVC base plate and pressed on the NC membrane, and the conjugation pad overlaps the NC membrane by 2 mm; the sample pad is adhered to the conjugation pad, and the sample pad overlaps the conjugation pad by 2 mm; the test line and the quality control line are both arranged on the NC membrane.

[0008] Preferably, the test line is a line drawn on the NC membrane by the FMDV-O-VP1 antigen; the quality control line is a line drawn on the NC membrane by the goat anti-chicken IgG antibody, and the distance between the test line and the quality control line is 3.5 mm.

[0009] Preferably, the plastic card shell includes an upper cover and a lower mounting box; the upper cover is covered on the lower mounting box, and the upper cover and the lower mounting box are detachably connected by a connecting piece; a fixing groove is provided in the lower mounting box; the PVC base plate is placed in the fixing groove, and the depth of the fixing groove is the same as the thickness of the PVC base plate.

[0010] Preferably, the upper cover is provided with a sample loading hole and an observation window; a first pressing step is provided in the sample loading hole; a second pressing step is provided in the observation window; the sample loading hole and the observation window are both opposite to the fixing groove, and the first pressing step is used to press the sample pad in the fixing groove, and the second pressing step is used to press the NC membrane in the fixing groove; a notch is provided at the edge adjacent to the first pressing step and the second pressing step, and the two notches are located on the same straight line.

[0011] Preferably, the connecting part includes a rubber belt and a staple; there are at least four rubber belts, which are arranged on both sides of the upper cover, the top of the rubber belt is fixedly connected to the two sides of the upper cover, and the lower end of the rubber belt is provided with a snap-in hole; the staples are arranged on both sides of the lower mounting box, and the staples and the snap-in holes form a snap connection.

[0012] Preferably, there are at least two snap-fit ​​holes, which are distributed along the vertical direction of the lower end of the rubber belt.

[0013] Preferably, the four corners below the upper cover are provided with clamping columns; the four corners below the lower installation box are provided with clamping slots; and the clamping columns and the clamping slots form a plug-in fit.

[0014] Preferably, a through hole is provided at the bottom of the fixing groove.

[0015] Preferably, the conjugate pad is a fluorescent microsphere conjugate pad.

[0016] The beneficial effects of the present invention are as follows:

[0017] (1) The kit provided by the utility model is used for the detection of antibodies to foot-and-mouth disease virus, which greatly shortens the detection time, reduces the detection cost, and improves the detection sensitivity. The portable fluorescence detector is used to interpret the detection results, which reduces the error in naked eye interpretation. Compared with similar methods, it has the advantages of a simple labeling process, high sensitivity, and accurate results.

[0018] (2) The plastic card shell provided by the present invention can press and fix the test strip through the first pressing step and the second pressing step, and the pressing force forms an adjustable mode through the connecting piece, and forms a chromatography channel after titration through the notch, thereby improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural diagram of the utility model;

[0020] Figure 2 It is a side view of the utility model;

[0021] Figure 3 This is a top view of the lower installation box of the utility model;

[0022] Figure 4 This is a schematic diagram of the test strip structure of the utility model.

[0023] Description of reference numerals:

[0024] 10. Upper cover; 11. Sample loading hole; 111. First pressing step; 12. Observation window; 121. Second pressing step; 13. Clamping column; 20. Lower mounting box; 21. Fixing slot; 22. Through hole; 23. Clamping slot; 31. PVC bottom plate; 32. Sample pad; 33. Conjugation pad; 34. NC membrane; 342. Quality control line; 35. Absorbent pad; 341. Test line; 41. Rubber belt; 411. Snap-in hole; 42. Pin. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. In the absence of conflict, the embodiments in this application and the features in the embodiments can be combined with each other. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] Example

[0027] See also Figure 1-4 As shown:

[0028] The utility model provides an antigen detection kit, which comprises a detection reagent card, an ultraviolet laser pen, a sample extract and a dropper.

[0029] The detection reagent card includes a plastic card shell and a test strip 30. The test strip 30 is used to detect foot-and-mouth disease virus in the sample serum to be tested and is inserted into the plastic card shell. The test strip 30 is provided with a detection line 341 and a quality control line 342 for observing the test results.

[0030] The dropper is used to draw up the sample extract and the sample serum to be tested and drop them onto the test strip 30 in the plastic cartridge. After the sample extract and the sample serum to be tested are dropped onto the test strip 30, they undergo a chromatographic reaction and produce fluorescent bands at the test line 341 and the quality control line 342 on the strip.

[0031] The ultraviolet laser pen is used to illuminate the fluorescent strips generated at the detection line 341 and / or the quality control line 342, and is used to assist in observing the detection results of the detection reagent card on the sample serum to be detected.

[0032] The test strip 30 includes a PVC base 31, a sample pad 32, a NC membrane 34, a conjugation pad 33, and a water-absorbing pad 35. The PVC base 31 is snap-fitted to the plastic housing; the NC membrane 34 is adhered to the upper surface of the middle portion of the PVC base 31; the water-absorbing pad 35 is adhered to one end of the PVC base 31 and covers the NC membrane 34, with the water-absorbing pad 35 overlapping the NC membrane 34 by 2 mm. The conjugation pad 33 is adhered to the other end of the PVC base 31 and pressed against the NC membrane 34, with the conjugation pad 33 overlapping the NC membrane 34 by 2 mm. The conjugation pad 33 is a fluorescent microsphere conjugation pad. The sample pad 32 is adhered to the conjugation pad 33, with the sample pad 32 overlapping the conjugation pad 33 by 2 mm. The test line 341 and the quality control line 342 are both provided on the NC membrane 34.

[0033] The detection line 341 is the FMDV-O-VP1 antigen drawn on the NC membrane 34; the quality control line 342 is the sheep anti-chicken IgG antibody drawn on the NC membrane 34, and the distance between the detection line 341 and the quality control line 342 is 3.5 mm.

[0034] The plastic card housing includes an upper cover 10 and a lower mounting box 20 .

[0035] The upper cover 10 is placed on the lower mounting box 20, and the upper cover 10 and the lower mounting box 20 are detachably connected via a connector. The lower mounting box 20 has a fixing groove 21. The PVC base plate 31 is placed in the fixing groove 21, and the depth of the fixing groove is the same as the thickness of the PVC base plate 31.

[0036] The upper cover 10 is provided with a sample loading port 11 and an observation window 12. A first pressing step 111 is provided in the sample loading port 11, and a second pressing step 121 is provided in the observation window 12. Both the sample loading port 11 and the observation window 12 face the fixed groove 21. The first pressing step 111 is used to press the sample pad 32 in the fixed groove 21, while the second pressing step 121 is used to press the NC membrane 34 in the fixed groove 21. Notches are provided at the adjacent edges of the first pressing step 111 and the second pressing step 121, and the two notches are aligned, thus forming a chromatographic directional channel after titration.

[0037] The connectors include rubber bands 41 and staples 42. The rubber bands 41 are made of rubber and are provided on either side of the upper cover 10. The top ends of the rubber bands 41 are fixedly connected to the sides of the upper cover 10, and the lower ends of the rubber bands 41 are provided with engaging holes 411. The staples 42 are provided on either side of the lower mounting box 20 and engage with the engaging holes 411. There are at least two engaging holes 411, distributed vertically along the lower ends of the rubber bands 41.

[0038] By adjusting the different engaging holes 411 on the rubber band 41 and engaging the engaging pins 42 , the first pressing step 111 and the second pressing step 121 exert different pressing forces on the test strip 30 , thereby meeting the different fixing forces required for the test strip 30 during chromatographic testing.

[0039] There are clamping columns 13 at the four corners below the upper cover 10; there are clamping slots 23 at the four corners of the lower installation box 20; the clamping columns 13 and the clamping slots 23 form a plug-in fit, so that no misalignment occurs when the connecting piece connects the upper cover 10 and the lower installation box 20.

[0040] A through hole 22 is defined at the bottom of the fixing groove 21 .

[0041] In actual use, the sample extract and the sample serum to be tested are dropped into the sample addition hole 11 through a dropper, and the fluorescent strips generated at the detection line 341 and the quality control line 342 on the test strip 30 are observed through the observation window 12.

[0042] A through hole 22 is provided at the bottom of the fixing groove 21 , through which the operator can use a finger or other object to easily push the test strip 30 out of the fixing groove 21 , thereby quickly replacing the test strip 30 and saving time.

[0043] Also includes waste sealing bag and desiccant.

[0044] Example 2

[0045] How to use the kit:

[0046] The method of using the kit includes the following steps:

[0047] S1: Take out the sample extract;

[0048] S2: preparing a test strip 30 for virus detection, and assembling the test strip 30 into a detection reagent card for detection;

[0049] Step S2 includes:

[0050] S21. The nitrocellulose membrane, ie, the NC membrane 34 is attached to the middle of the upper surface of the PVC base plate 31;

[0051] S22. FMDV-O-VP1 antigen and goat anti-chicken IgG antibody were streaked on the NC membrane 34 as the test line 341 and the quality control line 342, with the two bands separated by 3.5 mm;

[0052] S23. The marked PVC base plate 31 is placed in an incubator to dry for 30 min;

[0053] S24. Attach the absorbent pad 35 to one end of the PVC base plate 31 and overlay it on the NC membrane 34, overlapping it by 2 mm. Attach the conjugate pad 33 to the other end of the PVC base plate 31 and also press it onto the NC membrane 34, overlapping it by 2 mm. Attach the sample pad 32 to the conjugate pad 33, overlapping it by 2 mm.

[0054] S25. The PVC substrate 31 is pasted with a cutting machine to cut into 9mm test strips 30, placed in a bag, sealed and stored at room temperature after adding a desiccant;

[0055] S26. Place the test strip 30 in the fixing groove 21 of the bottom plate of the plastic card shell, cover the upper cover 10 and the lower mounting box 20, and put the same extract into an aluminum foil bag and store it at room temperature.

[0056] S3: Drop the sample extract and the sample serum to be tested into the sample well 11 of the detection reagent card to detect viral antibodies in the sample serum;

[0057] Step S3 includes:

[0058] S31. Open the foil bag along the tear, take out the test card and place it flat on a horizontal table;

[0059] S32. Add 10 μl of the sample serum solution to be tested into the sample well 11 of the test card, and then add 4 drops of sample extract.

[0060] S4: Use an ultraviolet laser pen to illuminate the test strip 30 of the detection reagent card, and obtain the test result of the sample serum virus antibody based on the fluorescent strip on the test strip 30.

[0061] Step S4 includes:

[0062] S41. After waiting for 15 minutes, illuminate the test card with a UV laser pen and observe the color development results of the test line 341 and the quality control line 342;

[0063] S42. Determine the test result of the sample serum to be tested based on the color development result;

[0064] The test results include positive, negative, and invalid. A positive result is when a fluorescent reaction line appears at the test line 341 and a fluorescent reaction line appears at the quality control line 342. A negative result is when only a fluorescent reaction line appears at the quality control line 342. An invalid result is when no fluorescent reaction line appears at the quality control line 342.

[0065] It should be noted that if the embodiments of the present invention involve directional indications such as up, down, left, right, front, back, etc., then such directional indications are only used to explain the relative positional relationship and movement of the various components in a specific posture as shown in the accompanying drawings. If the specific posture changes, the directional indications will also change accordingly. In addition, if the embodiments of the present invention involve descriptions such as "first" and "second", then such descriptions such as "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features specified as "first" and "second" can explicitly or implicitly include at least one of such features. In addition, the meaning of "and / or" appearing throughout the text includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, solution B, or solutions where both A and B are satisfied. In addition, "multiple" refers to more than two. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that ordinary technicians in this field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist.

[0066] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An antigen detection kit, characterized in that include: A detection reagent card, an ultraviolet laser pen, a sample extract and a dropper; the detection reagent card comprises a plastic card shell and a test strip (30); the test strip (30) is used to detect foot-and-mouth disease virus in a sample serum to be detected and is inserted into the plastic card shell, and a detection line (341) and a quality control line (342) for observing the detection result are provided on the test strip (30); the dropper is used to absorb the sample extract and the sample serum to be detected and drop them onto the test strip (30) in the plastic card shell; the ultraviolet laser pen is used to irradiate the fluorescent strips produced on the detection line (341) and / or the quality control line (342) by the sample extract and the sample serum to be detected after a chromatographic reaction.

2. An antigen detection kit according to claim 1, characterized in that, The test strip (30) includes a PVC base plate (31), a sample pad (32), an NC membrane (34), a conjugation pad (33) and a water-absorbing pad (35); the PVC base plate (31) is snap-fitted with a plastic card shell; the NC membrane (34) is pasted on the upper surface of the middle part of the PVC base plate (31); the water-absorbing pad (35) is pasted on one end of the PVC base plate (31) and covers the NC membrane (34), and the water-absorbing pad (35) overlaps with the NC membrane (34) by 2 mm; the conjugation pad (33) is pasted on the other end of the PVC base plate (31) and pressed on the NC membrane (34), and the conjugation pad (33) overlaps with the NC membrane (34) by 2 mm; the sample pad (32) is pasted on the conjugation pad (33), and the sample pad (32) overlaps with the conjugation pad (33) by 2 mm; the detection line (341) and the quality control line (342) are both arranged on the NC membrane (34).

3. An antigen detection kit according to claim 2, characterized in that, The detection line (341) is a line drawn on the NC membrane (34) by the FMDV-O-VP1 antigen; the quality control line (342) is a line drawn on the NC membrane (34) by the sheep anti-chicken IgG antibody, and the distance between the detection line (341) and the quality control line (342) is 3.5 mm.

4. An antigen detection kit according to claim 2 or 3, characterized in that, The plastic card shell comprises an upper cover (10) and a lower installation box (20); the upper cover (10) is covered on the lower installation box (20), and the upper cover (10) and the lower installation box (20) are detachably connected via a connecting piece; a fixing groove (21) is provided in the lower installation box (20); the PVC bottom plate (31) is placed in the fixing groove (21), and the depth of the fixing groove (21) is the same as the thickness of the PVC bottom plate (31).

5. An antigen detection kit according to claim 4, characterized in that, The upper cover (10) is provided with a sample loading hole (11) and an observation window (12); a first pressing step (111) is provided in the sample loading hole (11); a second pressing step (121) is provided in the observation window (12); the sample loading hole (11) and the observation window (12) are both opposite to the fixing groove (21), and the first pressing step (111) is used to press the sample pad (32) in the fixing groove (21), and the second pressing step (121) is used to press the NC membrane (34) in the fixing groove (21); notches are provided at the edges adjacent to the first pressing step (111) and the second pressing step (121), and the two notches are located on the same straight line.

6. An antigen detection kit according to claim 5, characterized in that The connecting member comprises a rubber belt (41) and a clamping nail (42); there are at least four rubber belts (41) and they are arranged on both sides of the upper cover (10); the top end of the rubber belt (41) is fixedly connected to both sides of the upper cover (10); the lower end of the rubber belt (41) is provided with a clamping hole (411); the clamping nail (42) is arranged on both sides of the lower installation box (20), and the clamping nail (42) and the clamping hole (411) form a clamping connection.

7. An antigen detection kit according to claim 6, characterized in that: There are at least two snap-fit ​​holes (411), which are distributed along the vertical direction of the lower end of the rubber belt (41).

8. An antigen detection kit according to claim 5, characterized in that: The four corners below the upper cover (10) are provided with clamping columns (13); the four corners of the lower installation box (20) are provided with clamping slots (23); the clamping columns (13) and the clamping slots (23) form a plug-in fit.

9. An antigen detection kit according to claim 8, characterized in that: A through hole (22) is provided at the bottom of the fixing groove (21).

10. An antigen detection kit according to claim 3, characterized in that: The binding pad (33) is a fluorescent microsphere binding pad.

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