Amplification device for nucleic acid detection

Abstract

The utility model provides an amplification device for nucleic acid detection, and relates to the technical field of nucleic acid amplification, the amplification device comprises a frame body, the right side of the upper end of the frame body is provided with a water cooling head, the upper end surface of the water cooling head is provided with a Peltier, the top of the Peltier is provided with a fluorescent quantitative plate, and the middle part of the upper end of the frame body is movably provided with a movable frame; a film sealing hot cover is arranged at the bottom of the inner side of the movable frame; and the lifting driving assembly is used for driving the film sealing hot cover to do vertical lifting motion, the lifting driving assembly comprises a servo motor, the servo motor is fixedly installed at the left end of the inner side of the movable frame, a gear is arranged at the output end of the servo motor, and the side end of the gear is connected with a rack in a meshed mode. The amplification device provided by the utility model realizes film sealing and PCR functions, and has the advantages of stronger instrument compatibility, simpler experimental operation, more reliable PCR result and better stability; the film has a heat sealing function and is matched with an aluminum foil heat sealing film, so that the effects of high efficiency, low cost, tight film sealing and evaporation prevention are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid amplification, in particular to an amplification device for nucleic acid detection. Background Art

[0002] Nucleic acid amplification devices are laboratory devices used to amplify specific DNA or RNA sequences. They have a wide range of applications in molecular biology, clinical diagnostics, forensic medicine, and other fields. These devices typically simulate polymerase chain reaction (PCR) or other nucleic acid amplification techniques to rapidly replicate trace amounts of nucleic acid samples for subsequent testing and analysis.

[0003] To this end, we propose an amplification device for nucleic acid detection for amplifying nucleic acids. Utility Model Content

[0004] In order to solve the problems in the background technology, the utility model provides an amplification device for nucleic acid detection.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] An amplification device for nucleic acid detection comprises a frame, a water-cooling head is provided on the right side of the upper end of the frame, a Peltier is placed on the upper end surface of the water-cooling head, a fluorescence quantitative plate is installed on the top of the Peltier, a movable frame is movably installed in the middle of the upper end of the frame, and a film-sealing thermal cover is provided at the inner bottom of the movable frame; a lifting drive component, the lifting drive component is used to drive the film-sealing thermal cover to perform vertical lifting movement, the lifting drive component comprises a servo motor, the servo motor is fixedly mounted on the inner left end of the movable frame, the output end of the servo motor is provided with a gear, the side end of the gear is meshedly connected with a rack, a lifting platform is provided in the middle of the bottom end of the rack, and the film-sealing thermal cover is fixedly mounted in the middle of the bottom end of the lifting platform.

[0007] Preferably, a horizontal drive component is used to drive the film sealing thermal cover to slide horizontally. The horizontal drive component includes a through-axis screw motor, which is fixedly installed on the left side of the upper end of the frame. A movable frame is provided at the right end of the screw of the through-axis screw motor, and the movable frame is fixedly connected to the movable frame.

[0008] Preferably, negative pressure air ducts are provided in the middle of both ends of the fluorescent quantitative plate, two groups of the negative pressure air ducts are respectively installed on the inner walls of both sides of the frame, and the negative pressure air ducts are connected to the suction pump through a pipeline.

[0009] Preferably, guide rails are provided on both sides of the upper end of the frame body, and slide rails are provided on both sides of the bottom end of the movable frame, and the slide rails are slidably connected to the guide rails.

[0010] Preferably, guide posts are provided at the four corners of the upper end of the film-sealing thermal cover, four groups of the guide posts are mounted on the inner top of the movable frame, and the four groups of the guide posts are movably connected to the lifting platform.

[0011] Preferably, a top cover is provided at the middle of the top end of the movable frame.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] The amplification device proposed in this solution combines sealing and PCR functions, resulting in greater instrument compatibility, simpler experimental operation, more reliable PCR results, and improved stability. Its built-in heat-sealing function, combined with aluminum foil heat-sealing film, provides efficient and low-cost sealing to prevent evaporation.

[0014] In addition, the setting of the horizontal drive component and the lifting drive component improves the accuracy of the film sealing hot cover movement and the hot pressing film sealing. The entire amplification process is simple to operate with high precision, achieving a good film sealing effect. At the same time, by setting negative pressure air ducts in the middle of both ends of the fluorescence quantitative plate, during the amplification process, the suction pump is started and the evaporated gas can be sucked out through the negative pressure air duct and discharged outside the instrument, thereby effectively avoiding the occurrence of aerosol contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0016] Figure 2 It is an exploded view of the utility model;

[0017] Figure 3 This is an exploded view of the sealing film thermal cover structure of the present invention;

[0018] Figure 4 It is a structural diagram of the horizontal drive component in the utility model.

[0019] In the figure: 1. Frame; 2. Peltier; 3. Fluorescent quantitative plate; 4. Negative pressure air duct; 5. Guide rail; 6. Movable frame; 7. Slide rail; 8. Lifting platform; 9. Sealing thermal cover; 10. Guide column; 11. Rack; 12. Gear; 13. Servo motor; 14. Top cover; 15. Movable frame; 16. Through-axis screw motor; 17. Water cooling head. DETAILED DESCRIPTION

[0020] The technical solution in the embodiments of the present application is to solve the problems of the above-mentioned background technology, and the overall idea is as follows:

[0021] Example: Refer to Figure 1 - Figure 4As shown, an amplification device for nucleic acid detection in this embodiment includes a frame 1, a water-cooled head 17 is provided on the right side of the upper end of the frame 1, a Peltier 2 is placed on the upper end surface of the water-cooled head 17, and a fluorescent quantitative plate 3 is installed on the top of the Peltier 2. The temperature accuracy and uniformity of the water-cooled head 17 are better, mainly by taking away heat through liquid circulation, which has a good and uniform heat dissipation and cooling effect for the bottom of the Peltier 2. The Peltier 2 mainly plays a heating role. The fluorescent quantitative plate 3 is an existing mature component, and the specific model needs to be selected according to the specifications of the well plate to be amplified. The fluorescent quantitative plate 3 of the utility model is adapted to 96 amplification well plates, so that the staff can stably place the 96 amplification well plates to be amplified on the fluorescent quantitative plate 3 for amplification. A movable frame 6 is movably installed in the middle of the upper end of the frame 1, and a sealing film heat cover 9 is provided on the inner bottom of the movable frame 6;

[0022] The lifting drive assembly is used to drive the film sealing heat cover 9 to perform vertical lifting movement. The lifting drive assembly includes a servo motor 13, which is fixedly mounted on the inner left end of the movable frame 6. The output end of the servo motor 13 is provided with a gear 12, and the side end of the gear 12 is meshed and connected with a rack 11. The middle part of the bottom end of the rack 11 is provided with a lifting platform 8, and the film sealing heat cover 9 is fixedly mounted on the bottom middle part of the lifting platform 8; the horizontal drive assembly is used to drive the film sealing heat cover 9 to slide horizontally. The horizontal drive assembly includes a through-axis screw motor 16, which is fixedly mounted on the upper left side of the frame body 1, and the right end of the screw of the through-axis screw motor 16 is provided with a movable frame 15, which is fixedly connected to the movable frame 6.

[0023] Among them, the through-axis screw motor 16 is started to drive the screw to move, thereby pushing the movable frame 15, thereby pushing the movable frame 6 to move horizontally, so that the film sealing thermal cover 9 can be moved to the upper position of the fluorescence quantitative plate 3, and then the servo motor 13 is started to drive the gear 12 to rotate. Since the gear 12 is engaged with the rack 11, when the gear 12 rotates, it can drive the rack 11 to move up and down, thereby driving the film sealing thermal cover 9 to move up and down and slide. When the rack 11 descends, the film sealing thermal cover 9 can descend to cover the surface of the 96-well amplification plate, and then the 96-well amplification plate is sealed.

[0024] Negative pressure air ducts 4 are provided in the middle of both ends of the fluorescence quantitative plate 3. Two sets of negative pressure air ducts 4 are respectively installed on the inner walls of both sides of the frame 1. The negative pressure air ducts 4 are connected to the suction pump through a pipe. During the amplification process, aerosol contamination is prone to occur. At this time, the suction pump is started and the evaporated gas can be sucked out through the negative pressure air duct 4 and discharged to the outside of the instrument, thereby effectively avoiding the occurrence of contamination.

[0025] Guide pillars 10 are provided at the four corners of the upper end of the film sealing thermal cover 9. Four groups of guide pillars 10 are installed on the inner top of the movable frame 6. The four groups of guide pillars 10 are movably connected to the lifting platform 8. The four groups of guide pillars 10 are used for limiting, thereby effectively improving the stability of the lifting platform 8 when driving the film sealing thermal cover 9 to slide vertically, avoiding the film sealing thermal cover 9 from deflecting during lifting, which affects the film sealing work.

[0026] In some instances, guide rails 5 are provided on both sides of the upper end of the frame 1, and slide rails 7 are provided on both sides of the bottom end of the movable frame 6. The slide rails 7 are slidably connected to the guide rails 5 to guide the movement of the movable frame 6, thereby effectively improving the stability of the movable frame 6 when sliding horizontally, thereby improving the accuracy of the film sealing hot cover 9 in moving the film.

[0027] In some examples, a top cover 14 is provided at the middle of the top end of the movable frame 6 to cover and protect the film sealing thermal cover 9 .

[0028] The working principle of this utility model is:

[0029] During use, the specific steps are as follows:

[0030] The first step is to place the 96-well amplification plate to be tested for nucleic acid on the fluorescence quantitative plate 3, and then start the through-axis screw motor 16 to make the screw translate, thereby pushing the movable frame 15. Under the thrust of the movable frame 15, the movable frame 6 moves horizontally, thereby moving the film-sealing hot cover 9 to the top of the 96-well amplification plate; then start the servo motor 13, and the servo motor 13 drives the gear 12 to rotate, thereby driving the rack 11 to move up and down, and the rack 11 descends to drive the lifting platform 8 and the film-sealing hot cover 9 to press down and cover the 96-well amplification plate, thereby achieving the first film sealing of the 96-well amplification plate. After the film sealing is completed, the servo motor 13 and the through-axis screw motor 16 are driven in the reverse direction, thereby driving the film-sealing hot cover 9 to return to the initial position;

[0031] In the second step, an external pipette draws the extract from the extraction module and adds it to the 96-well amplification plate. After the addition is completed, the first step is repeated to drive the sealing thermal cover 9 to move to the top of the 96-well amplification plate and press down to perform the second sealing.

[0032] At this time, the Peltier 2 at the bottom of the fluorescence quantitative plate 3 is heated, and the coolant inside the water-cooling head 17 begins to circulate, and the first amplification begins. After waiting for a period of time, the first amplification is completed and all mechanisms return to their initial positions.

[0033] In the third step, the pipette draws the hybridization solution from the hybridization module and adds it to the 96-well amplification plate. During the puncture process of the pipette, aerosol contamination may occur. At this time, the suction pump is started to suck out the evaporated gas through the negative pressure air duct 4 and discharge it from the entire instrument. After the hybridization solution is added, the first step is repeated. The servo motor 13 and the through-axis screw motor 16 drive the sealing hot cover 9 to move to the top of the 96-well amplification plate and press it down to perform the third sealing. At the same time, the Peltier 2 at the bottom of the 3 fluorescence quantitative plate is heated again, and the coolant inside the water-cooling head 17 begins to circulate, and the second amplification begins. After waiting for a while, the second amplification is completed, all mechanisms return to their initial positions, and the amplification process ends.

[0034] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed in this invention is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid detection amplification device, characterized in that: The invention comprises a frame (1), a water cooling head (17) is provided on the right side of the upper end of the frame (1), a Peltier (2) is placed on the upper end surface of the water cooling head (17), a fluorescence quantitative plate (3) is installed on the top of the Peltier (2), a movable frame (6) is movably installed in the middle of the upper end of the frame (1), and a sealing film heat cover (9) is provided on the inner bottom of the movable frame (6); A lifting drive assembly, the lifting drive assembly is used to drive the film sealing thermal cover (9) to perform vertical lifting movement, the lifting drive assembly includes a servo motor (13), the servo motor (13) is fixedly mounted on the inner left end of the movable frame (6), the output end of the servo motor (13) is provided with a gear (12), the side end of the gear (12) is meshedly connected with a rack (11), a lifting platform (8) is provided at the middle of the bottom end of the rack (11), and the film sealing thermal cover (9) is fixedly mounted at the middle of the bottom end of the lifting platform (8); A horizontal drive assembly is used to drive the film sealing heat cover (9) to slide in the horizontal direction. The horizontal drive assembly includes a through-axis screw motor (16). The through-axis screw motor (16) is fixedly installed on the left side of the upper end of the frame (1). The right end of the screw of the through-axis screw motor (16) is provided with a movable frame (15). The movable frame (15) is fixedly connected to the movable frame (6).

2. A nucleic acid detection amplification device according to claim 1, characterized in that: Negative pressure air ducts (4) are provided in the middle of both ends of the fluorescent quantitative plate (3), and two groups of the negative pressure air ducts (4) are respectively installed on the inner walls of both sides of the frame (1), and the negative pressure air ducts (4) are connected to the suction pump through a pipeline.

3. A nucleic acid detection amplification device according to claim 2, characterized in that: Guide rails (5) are provided on both sides of the upper end of the frame body (1), and slide rails (7) are provided on both sides of the lower end of the movable frame (6), and the slide rails (7) are slidably connected to the guide rails (5).

4. A nucleic acid detection amplification device according to claim 3, characterized in that: Guide columns (10) are provided at the four corners of the upper end of the film sealing heat cover (9), and four groups of the guide columns (10) are installed on the inner top of the movable frame (6). The four groups of the guide columns (10) are movably connected to the lifting platform (8).

5. The nucleic acid detection amplification device according to claim 4, characterized in that: A top cover (14) is provided at the middle portion of the top end of the movable frame (6).

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