Nanopore detector

Abstract

The utility model belongs to the technical field of biological detection, and particularly relates to a nanopore detector which comprises a detection main body, a lower shell detachably connected with the detection main body, and an upper sealing cover rotationally arranged on the detection main body, the detection main body comprises a mounting plate, a first surface of the mounting plate is provided with a mounting position for mounting a sequencing chip, two sides of the mounting position are symmetrically provided with a first adapter plate and a second adapter plate, and the first adapter plate and the second adapter plate are connected with the chip through connector elastic sheets; the second surface of the mounting plate is detachably provided with a main control board through a connecting plate with a U-shaped cross section, the second surface of the mounting plate is symmetrically provided with at least two first connectors relative to the mounting position, and the main control board is provided with at least two second connectors corresponding to the first connectors; according to the scheme, a reverse installation structure is adopted, a low-cost detection system is provided, and the detection requirements of small enterprises and public service institutions are met.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological detection, and particularly relates to a nanopore detector. Background Art

[0002] Nanopore detectors are an advanced biomedical engineering application that utilizes nanopore technology for gene sequencing and single-molecule detection. Nanopore detectors are primarily categorized into two types: biological nanopores and solid-state nanopores. Through their unique technologies and applications, they play an important role in gene sequencing, single-molecule detection, and other fields, providing new tools and methods for life science research and clinical medicine.

[0003] Before using a nanopore detector, the chip must be placed and secured on the instrument before testing can begin. However, most current chip fixtures only apply unidirectional force to the clamping devices during fixation, resulting in poor fixation and sealing reliability, affecting experimental accuracy. A few bolted clamping devices lack positioning mechanisms, making them prone to misalignment during installation, causing the solution in the liquid reservoir to leak out from between the two reservoirs after the device is secured. Furthermore, the inconvenience of bolt installation is a significant issue.

[0004] There are two known ways to place the chip in a sequencer. One is to build the chip into the entire instrument, which can effectively shield the signal but is inconvenient to operate. The other is to raise the chip above the operating platform, which may cause interference to a certain extent.

[0005] In addition, in order to pursue a small size while being able to detect high-throughput chips, the nanopore detectors in the existing technology will use a highly integrated main control chip for data acquisition and data processing. However, the high-integration main control chip is expensive, making the cost of the entire device as high as tens of millions. For example, the Chinese utility model patent with application number 201510378422.7 discloses a high-throughput gene sequencing dynamic scheduling control method and system device. Since high-throughput gene sequencing has high precision requirements in the entire sequencing process, the equipment in this solution will also be more complex and costly.

[0006] However, for small businesses and public service testing and medical institutions, the cost is too high, and the number of channels of the chips used by small businesses and public service testing and medical institutions is usually around 300, and there is no need to design too many testing chips with a higher number of channels. Therefore, there is a need to provide a low-cost testing device that is more suitable for small businesses and public service institutions. Utility Model Content

[0007] The purpose of the present utility model is to provide a nanopore detector to partially alleviate or solve at least one of the above problems, and to provide a low-cost detection device that is more suitable for small enterprises and public service agencies, and has high production efficiency and is convenient for later debugging and maintenance of the detector.

[0008] In order to solve the above-mentioned technical problems, the present invention specifically adopts the following technical solutions: a nanopore detector, comprising a detection body, a lower shell detachably connected to the detection body, and an upper cover rotatably arranged on the detection body;

[0009] The detection body includes a mounting plate, a first surface of the mounting plate is provided with a mounting position for mounting a sequencing chip, a first adapter plate and a second adapter plate are symmetrically provided on both sides of the mounting position, and the first adapter plate and the second adapter plate are connected to the chip via connector springs; a main control board is detachably provided on the second surface of the mounting plate via a connecting plate with a concave cross-section, and at least two first connectors are symmetrically provided on the second surface of the mounting plate about the mounting position, and at least two second connectors corresponding to the first connectors are provided on the main control board;

[0010] After the pins on the bottom side of the PCB boards are respectively inserted into the second connector to be electrically connected to the main control board, the connecting board is installed on the mounting board so that the pins on the top side of the PCB boards are inserted into the first connector.

[0011] As an improvement, an air inlet is provided on the front side wall of the lower shell in an area corresponding to the main control board, and an air outlet is provided on the rear side wall of the lower shell in an area corresponding to the PCB board. The horizontal plane position of the air outlet is higher than the horizontal plane position of the air inlet, and a fan for extracting hot air from the shell is provided at the air outlet.

[0012] As an improvement, the number of the PCB boards is four, and two of them form a group, and the two groups are symmetrically arranged between the mounting board and the main control board.

[0013] As an improvement, a fixing plate is further provided on the first surface of the mounting plate, the first adapter plate and the second adapter plate are located between the fixing plate and the mounting plate, and the fixing plate is provided with through holes corresponding to the mounting positions.

[0014] As an improvement, the fixing plate is provided with a locking mechanism, the locking mechanism comprising a pressing member, a first side of the pressing member being rotatably provided on the fixing plate via a first rotating shaft, a second side of the pressing member being rotatably provided with a fastening member via a second rotating shaft, a first end of the fastening member being fixed to the pressing member via an elastic member; correspondingly, a fixing buckle cooperating with the fastening member is provided on the fixing plate;

[0015] When the locking mechanism is in a locked state, the pressing member and the mounting position together form a clamping structure for clamping the chip, and the fastening member hooks the fixing buckle;

[0016] When the first end of the fastening member is pressed, the second end of the fastening member rotates around the second rotating shaft and then disengages from the fixing buckle, thereby opening the locking mechanism.

[0017] As an improvement, a torsion spring is further provided on the first rotating shaft, and both ends of the torsion spring are respectively fixed to the pressing member;

[0018] When the locking mechanism is in a locked state, the torsion spring is in a compressed state; when the first end of the fastening member is pressed, the fastening member is disengaged from the fixing buckle, and the torsion spring returns to its initial state, thereby driving the pressing member back to an open state.

[0019] As an improvement, the pressing member is provided with a spring pin, and the locking mechanism, the spring pin and the fixing plate are all made of metal;

[0020] When the locking mechanism is in a locked state, the free end of the spring pin abuts against the fixing buckle, so that a conductor is formed between the locking mechanism and the fixing plate.

[0021] As an improvement, the height of the mounting position is smaller than the thickness of the chip, so that when the locking mechanism is in the locked state, the first surface of the pressing member abuts against the first surface of the chip.

[0022] As an improvement, a first magnetic block is provided on the upper cover, and a magnetic metal part cooperating with the first magnetic block is provided on the fixing plate.

[0023] As an improvement, the main control board is horizontally arranged on the connecting board.

[0024] The principle and beneficial effects of the present invention are:

[0025] To meet the testing needs of small businesses and public service agencies, this solution proposes a low-cost testing system. This solution uses a "reverse installation" structure, so that during the assembly process, the PCB board is first inserted into the second connector and connected to the main control board. It is then fixed to the mounting plate via the connecting plate and connected to the first connector to form a pre-installed state. After testing and adjustment, it is assembled with the lower housing to complete the assembly of the entire machine. This greatly facilitates production line work, effectively improves production assembly efficiency and reduces production costs while meeting its functional requirements. At the same time, during subsequent maintenance or overhaul, only the lower housing needs to be removed. The equipment is simple and easy to use.

[0026] In the prior art, since a highly integrated main control board is used for data acquisition and data processing, a cooling fan is usually provided for the highly integrated main control board. However, in the present application, since multiple independent PCB boards are used for data acquisition and a main control board is provided for data processing, and multiple PCB boards are vertically installed above the main control board, it is not only costly but also unnecessary to provide a fan for each PCB board and the main control board (for example, when the detection system performs a single or several detections, there is no need to start the fan at all). Therefore, a specific airflow channel is designed in the present application for cooling. For example, a cold air inlet is provided at the front side of the horizontally installed main control board, and two fans are provided at the upper rear side of the vertically installed PCB board, thereby forming a low-inlet and high-outlet airflow channel in the device, which can take into account not only the main control board installed at the bottom, but also the vertically installed PCB board. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the embodiments or the description of the prior art. In all drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the various elements or parts are not necessarily drawn according to the actual scale. Obviously, the drawings described below are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without inventive work.

[0028] Figure 1a This is a schematic diagram of the three-dimensional structure of a nanopore detector in a sequencing state according to an exemplary embodiment of the present invention;

[0029] Figure 1b This is a schematic diagram of the three-dimensional structure of a nanopore detector according to an exemplary embodiment of the present invention in an open state (not working);

[0030] Figure 2This is a schematic diagram of the partial structure of the locking mechanism when rotating in an exemplary embodiment of the present utility model;

[0031] Figure 3a This is a schematic diagram of a locking mechanism of an exemplary embodiment of the present invention in an open state;

[0032] Figure 3b A schematic diagram of another angle when the locking mechanism of an exemplary embodiment of the present invention is in an open state;

[0033] Figure 3c This is an enlarged structural diagram of an exemplary embodiment of the present invention when the locking mechanism is in an open state;

[0034] Figure 4a This is a schematic diagram of a locking mechanism of an exemplary embodiment of the present invention in a closed state;

[0035] Figure 4b A schematic diagram of an amplifying mechanism when the locking mechanism of an exemplary embodiment of the present invention is in a closed state;

[0036] Figure 5a A schematic diagram of the placement of a detector chip in the prior art;

[0037] Figure 5b This is a schematic diagram of another detector chip placement position in the prior art;

[0038] Figure 6 A schematic diagram of the three-dimensional structure of a nanopore detector according to an exemplary embodiment of the present invention from another angle;

[0039] Figure 7 This is a schematic diagram of the bottom structure of a nanopore detector according to an exemplary embodiment of the present invention;

[0040] Figure 8 This is a schematic diagram of the internal structure of a nanopore detector according to an exemplary embodiment of the present invention;

[0041] Figure 9 A schematic diagram of the three-dimensional structure of a detection body according to an exemplary embodiment of the present utility model;

[0042] Figure 10 A schematic diagram of the three-dimensional structure of the detection body of an exemplary embodiment of the present utility model from another angle;

[0043] Figure 11 This is a schematic diagram of the three-dimensional structure of the detection body and the upper cover after assembly according to an exemplary embodiment of the present invention;

[0044] Figure 12 This is a schematic diagram of the three-dimensional structure of the lower shell of an exemplary embodiment of the present utility model;

[0045] Figure 13a This is a schematic diagram of the front structure of a sequencing chip according to an exemplary embodiment of the present invention;

[0046] Figure 13b This is a schematic diagram of the back structure of a sequencing chip according to an exemplary embodiment of the present invention;

[0047] Figure 14a A schematic diagram of the air duct flow direction of a detector according to an exemplary embodiment of the present invention;

[0048] Figure 14b A schematic diagram of the air duct flow direction of a detector from another angle according to an exemplary embodiment of the present invention;

[0049] Figure 14c This is a schematic diagram of the air duct flow direction at another angle of the detector according to an exemplary embodiment of the present invention.

[0050] Markings in the figure: 1. Detection body; 21. Mounting plate; 211. Mounting position; 22. Main control board; 231. First adapter board; 232. Second adapter board; 24. Fixing plate; 25. Connector spring; 26. Connecting plate; 27. PCB board; 28. First connector; 29. ​​Second connector; 2. Upper cover; 3. Lower shell; 31. Bottom cover; 4. Locking mechanism; 41. Pressing member; 42. Fastening member; 43. First rotating shaft; 44. Second rotating shaft; 45. Fixing buckle; 46. Elastic member; 47. Torsion spring; 48. Spring pin; 49. Articulated seat; 5. USB interface; 6. Duplex switch; 7. Air outlet; 11. Chip; 111. Observation window; 112. Liquid filling port; 113 Knob switch; 114. Spring contact area. DETAILED DESCRIPTION

[0051] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0052] Herein, the suffixes such as "module", "component" or "unit" used to represent elements are only used to facilitate the description of the present invention and have no specific meaning. Therefore, "module", "component" or "unit" can be used interchangeably.

[0053] As used herein, terms such as "upper," "lower," "inner," "outer," "front," "back," "one end," and "the other end" indicate positions or locations based on those shown in the accompanying drawings. These terms are intended solely to facilitate the description of the present invention and simplify the description. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0054] As used herein, unless otherwise expressly specified or limited, the terms "installed," "provided with," "connected," etc., should be understood broadly. For example, "connected" may refer to a fixed connection, a detachable connection, or an integral connection; it may refer to a mechanical connection, a direct connection, an indirect connection through an intermediate medium, or internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model on a case-by-case basis.

[0055] As used herein, "and / or" includes any and all combinations of one or more of the associated listed items.

[0056] Herein, "plurality" means two or more than two, ie, it includes two, three, four, five, etc.

[0057] This embodiment is basically as shown in Figure 1- Figure 14c As shown:

[0058] like Figure 1a and Figure 1b , the utility model provides a nanopore detection system, comprising a detection body, a lower shell detachably connected to the detection body, and an upper cover rotatably arranged on the detection body;

[0059] like Figure 8 and Figure 9 As shown, the detection body includes a mounting plate 21, and a mounting position for mounting a chip is provided on the first surface of the mounting plate 21. A first adapter plate 231 and a second adapter plate 232 are symmetrically provided on both sides of the mounting position. The first adapter plate 231 and the second adapter plate 232 are connected to the chip through a connector spring 25. A fixing plate is also provided on the first surface of the mounting plate 21. The first adapter plate 231 and the second adapter plate 232 are located between the fixing plate and the mounting plate 21. The fixing plate is provided with through holes corresponding to the mounting positions.

[0060] The main control board 22 is detachably mounted on the second surface of the mounting plate via a connecting plate 26 having a concave cross-section. For example, the main control board 22 is fixed to the connecting plate 26, and then the connecting plate 26 is fixed to the mounting plate 21 via bolts. The second surface of the mounting plate 21 is symmetrically provided with at least two first connectors 28 about the mounting position, and the main control board is provided with at least two second connectors 29 corresponding to the first connectors 28. When the PCB is mounted on the corresponding set of first connectors 28 and second connectors 29, the PCB is in a vertical position, that is, the PCB is parallel to two side walls of the lower housing.

[0061] When the connecting plate 26 is mounted on the mounting plate 21, the mounting plate 21, the connecting plate 26 and the main control board 22 together form an installation space for mounting a PCB board. Two opposite sides of the PCB board can be respectively inserted into a corresponding set of the first connector 28 and the second connector 29 for fixation.

[0062] Specifically, after the pins on the bottom side of multiple PCB boards are respectively inserted into the second connector to be electrically connected to the main control board, the connecting board is installed on the mounting board so that the pins on the top side of the PCB board are inserted into the first connector.

[0063] In some embodiments, four first connectors are provided, and correspondingly, four second connectors are also provided, for mounting four PCB boards, and the four PCB boards are arranged in groups of two symmetrically about the mounting position.

[0064] An air inlet is provided on the front side wall of the lower shell in an area corresponding to the main control board, and an air outlet is provided on the rear side wall of the lower shell in an area corresponding to the PCB board. The horizontal plane position of the air outlet is higher than the horizontal plane position of the air inlet, and a fan for extracting hot air from the shell is provided at the air outlet.

[0065] like Figure 14a-Figure 14c The air inlet is located at the front end of the detector, and an air inlet net is provided at the air inlet. The air outlet is located at the rear end of the detector, and a fan is provided at the rear end of the detector. The fan draws external air from the air inlet to the air outlet and discharges it. In this solution, the air outlet and the air inlet are set high and low, so that the wind can fully cover the surface of the PCB board during the flow process, thereby achieving comprehensive cooling.

[0066] When assembling the nanopore detector in this application, first insert one side of the PCB board into the first connector, then make the second connector on the main control board correspond to the other side of the PCB board, insert the PCB board into the second connector, and then install the connecting board on the mounting board, fix the PCB board and the main control board to form a detection body, which is the pre-installation structure mentioned in this article, to facilitate subsequent combination with the lower shell.

[0067] The detection system also includes a user interaction module that communicates data with the main control board, and is used for allowing the user to select an operating mode and input the number of channels of the sequencing chip currently undergoing batch testing; wherein the main control board includes:

[0068] A database for storing channel quantity groups and corresponding heat dissipation solutions obtained in advance through preliminary experiments; the heat dissipation solutions include: the speed of the fan, and the speed adjustment timing corresponding to the temperature rise stage and the target constant temperature stage;

[0069] The heat dissipation scheme matching module is used to match the corresponding channel number group and its corresponding heat dissipation scheme in the database according to the channel number input by the user after the user selects the batch test working mode;

[0070] The fan control module is used to control the operation of the two fans according to the matched heat dissipation solution.

[0071] In some embodiments, the fixing plate is provided with a locking mechanism, such as Figure 3a-Figure 4b As shown, the locking mechanism includes a pressing member 41, a first side of which is rotatably provided on the fixing plate 24 via a first rotating shaft 43, and a fastening member 42 is movably provided on the second side of the pressing member 41 via a second rotating shaft 44, and a first end of the fastening member 42 is fixed to the pressing member 41 via an elastic member 46; correspondingly, a fixing buckle 45 is provided on the fixing plate 24 to cooperate with the fastening member 42;

[0072] When the locking mechanism is in a locked state, the pressing member and the mounting position together form a clamping structure for clamping the chip, and the fastening member hooks the fixing buckle;

[0073] When the first end of the fastening member is pressed, the second end of the fastening member rotates around the second rotating shaft and then disengages from the fixing buckle, thereby opening the locking mechanism.

[0074] For example, in some embodiments, the elastic member is a spring, and the two ends of the spring are respectively fixed to the clamping member and the fastening member, the cross-section of the fastening member is approximately a "C-shaped" lock hook, and the second end of the fastening member and the contact portion of the fixed buckle are designed as a slope; when the fastening member contacts the side wall of the groove, the first end of the sloped portion of the fastening member first contacts the side wall of the groove, and during the rotation of the fastening member, the sloped portion of the fastening member moves along the side wall of the groove, so that the second end of the fastening member enters the interior of the groove. At this time, its slope design can play a guiding role, making the process of the fastening member entering the groove smoother.

[0075] In some embodiments, a groove for accommodating the second end of the fastening member is provided in the fixing buckle, and an opening for allowing the fastening member to extend into the groove is provided on the fixing buckle.

[0076] When the pressing member is pressed, the pressing member rotates around the first rotating shaft, and the second end of the fastening member contacts the side wall of the groove and rotates under the guidance of the first rotating shaft, while the elastic member is compressed; when the pressing member is pressed into place, the elastic member returns to its original position, driving the fastening member to return to its original position, and the second end of the fastening member abuts against the inner wall of the groove, so that the pressing member and the mounting position together form a clamping structure for clamping the chip;

[0077] When the first end of the fastening member is pressed, the elastic member is compressed, and the fastening member rotates around the second rotation axis, so that the fastening member can be separated from the fixing buckle.

[0078] In some embodiments, the groove includes a first groove arranged along the height direction of the sequencer, and a second groove formed by extending the first groove in the width direction of the sequencer, and the first end of the first groove is provided with an opening. When the pressing member is pressed to lock, the fastener is first pushed open along the side wall of the opening (at this time, the fastener rotates about the second rotating axis and the elastic member contracts), and the second end of the fastener extends into the first groove. When the inclined portion of the fastener fully enters the first groove, the fastener is no longer subjected to the force of the groove side wall (e.g., the side wall of the opening), and the fastener returns to its original position, enters the second groove, and hooks onto the inner wall of the second groove to achieve locking.

[0079] During specific use, when the chip needs to be pressed and fixed, the chip is placed in the installation position, and the second side of the pressing part is pressed to make the pressing part rotate around the first rotating shaft. Since the contact end of the fastening part and the fixing buckle adopts a bevel design, when the fastening part contacts the fixing buckle, the fastening part rotates clockwise around the second rotating shaft, and the spring is compressed at this time; when the pressing part is pressed into place, the second end of the fastening part is located inside the groove, and the spring reset will drive the fastening part to rotate counterclockwise and reset, and finally the second end of the fastening part hooks the fixing part to complete the entire locking action.

[0080] In some embodiments, a hinge seat is fixed on the fixing plate, and the first rotating shaft is rotatably disposed on the hinge seat.

[0081] In some embodiments, as Figure 3b , a torsion spring 47 is further provided on the first rotating shaft (specifically, it can be sleeved), and the two ends of the torsion spring 47 are respectively fixed to the two parts of the pressing member;

[0082] When the locking mechanism is in the locked state, the torsion spring is in a compressed state. When the first end of the fastening member is pressed, the fastening member disengages from the fixing buckle, and the torsion spring returns to its initial state, thereby driving the pressing member back to the open state. By providing a torsion spring, the pressing member can automatically rebound after the locking mechanism is opened and remain in the rebounded position without falling.

[0083] In some embodiments, the rotation angle range of the pressing member is 0°-90°, wherein when the pressing member is in the open state, the angle between the pressing member and the plane where the fixing plate is located is 60°-90°.

[0084] In some embodiments, the pressing member is provided with a spring pin, and the locking mechanism, the spring pin and the fixing plate are all made of metal;

[0085] When the locking mechanism is in a locked state, the free end of the spring pin rests against the fixing buckle. On the one hand, the spring pin can generate a buffering elastic force on the pressing member and the fixing plate when they contact each other, thereby reducing wear; on the other hand, it can form a conductor between the locking mechanism and the fixing plate, thereby shielding some interference signals to a certain extent and improving the detection accuracy of the detector.

[0086] In some embodiments, the fasteners and fixing buckles are made of stainless steel to ensure their strength requirements and prevent them from rapid wear and deformation during frequent locking and tightening. In addition, the remaining structures of the nanopore detector are made of aluminum alloy, which makes it lighter and has better electromagnetic shielding effect.

[0087] In some embodiments, shock-absorbing foam is provided on the fixing plate. The shock-absorbing foam is located at the first rotating shaft to prevent the pressing member from colliding with the fixing plate during rotation, thereby playing a role of buffering and silencing.

[0088] In some embodiments, the height of the mounting position is smaller than the thickness of the chip, so that when the locking mechanism is in a locked state, the first surface of the pressing member abuts against the first surface of the chip.

[0089] In the prior art, Figure 5a and Figure 5b Two chip installation methods are listed. One is to build the chip into the entire instrument. Although it can shield the signal, it is not convenient to operate. The other method is to raise the chip above the operating platform. This method will cause certain interference. The test body in this solution adopts a sunken design (setting a mounting position on the mounting plate), so that the first surface of the chip and the first surface of the fixing plate are located at the same horizontal plane, effectively avoiding the above problems.

[0090] Through the above locking mechanism, whether in the locking process or the unlocking process, you only need to press lightly (press the pressing part when locking, and press the first end of the fastening part when unlocking), which is convenient and quick to use.

[0091] In some embodiments, the upper cover is provided with a first magnetic block, and the fixing plate is provided with a magnetic metal member that cooperates with the first magnetic block. For example, the magnetic metal member can be an iron block, specifically a Q235 chrome-plated iron block. The upper cover is opened and closed by magnetic attraction, which is convenient and quick, and provides a good seal within the main body.

[0092] In summary, this application provides a low-cost "reverse installation" detector. The detection main body is pre-installed and combined into a whole, and then assembled with the lower shell, which greatly reduces the production cost and facilitates subsequent debugging and maintenance.

[0093] It should be noted that, in this document, the terms "comprises," "includes," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or apparatus comprising the element.

[0094] The embodiments of the present invention are described above in conjunction with the accompanying drawings, but the present invention is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present invention, ordinary technicians in this field can also make many forms without departing from the scope of protection of the purpose of the present invention and the claims, which are all within the protection of the present invention.

Claims

1. A nanopore detector, characterized in that: It includes a detection body, a lower shell detachably connected to the detection body, and an upper cover rotatably arranged on the detection body; The detection body includes a mounting plate, a first surface of the mounting plate is provided with a mounting position for mounting a sequencing chip, a first adapter plate and a second adapter plate are symmetrically provided on both sides of the mounting position, and the first adapter plate and the second adapter plate are connected to the chip via connector springs; a main control board is detachably provided on the second surface of the mounting plate via a connecting plate with a concave cross-section, and at least two first connectors are symmetrically provided on the second surface of the mounting plate about the mounting position, and at least two second connectors corresponding to the first connectors are provided on the main control board; After the pins on the bottom side of the PCB boards are respectively inserted into the second connector to be electrically connected to the main control board, the connecting board is installed on the mounting board so that the pins on the top side of the PCB boards are inserted into the first connector.

2. A nanopore detector according to claim 1, characterized in that: An air inlet is provided on the front side wall of the lower shell in an area corresponding to the main control board, and an air outlet is provided on the rear side wall of the lower shell in an area corresponding to the PCB board. The horizontal plane position of the air outlet is higher than the horizontal plane position of the air inlet, and a fan for extracting hot air from the shell is provided at the air outlet.

3. The nanopore detector according to claim 1, wherein: The number of the PCB boards is four, and two of them form a group, and the two groups are symmetrically arranged between the mounting board and the main control board.

4. The nanopore detector according to claim 1, wherein: A fixing plate is further provided on the first surface of the mounting plate. The first adapter plate and the second adapter plate are located between the fixing plate and the mounting plate. Through holes corresponding to the mounting positions are provided on the fixing plate.

5. The nanopore detector according to claim 4, characterized in that: The fixing plate is provided with a locking mechanism, which includes a pressing member, a first side of which is rotatably provided on the fixing plate via a first rotating shaft, and a fastening member is rotatably provided on the second side of the pressing member via a second rotating shaft, wherein a first end of the fastening member is fixed to the pressing member via an elastic member; correspondingly, a fixing buckle that cooperates with the fastening member is provided on the fixing plate; When the locking mechanism is in a locked state, the pressing member and the mounting position together form a clamping structure for clamping the chip, and the fastening member hooks the fixing buckle; When the first end of the fastening member is pressed, the second end of the fastening member rotates around the second rotating shaft and then disengages from the fixing buckle, thereby opening the locking mechanism.

6. The nanopore detector according to claim 5, characterized in that: The first rotating shaft is further provided with a torsion spring, and both ends of the torsion spring are respectively fixed to the pressing member; When the locking mechanism is in a locked state, the torsion spring is in a compressed state; when the first end of the fastening member is pressed, the fastening member is disengaged from the fixing buckle, and the torsion spring returns to its initial state, thereby driving the pressing member back to an open state.

7. The nanopore detector according to claim 6, characterized in that: The pressing member is provided with a spring pin, and the locking mechanism, the spring pin and the fixing plate are all made of metal; When the locking mechanism is in a locked state, the free end of the spring pin abuts against the fixing buckle, so that a conductor is formed between the locking mechanism and the fixing plate.

8. A nanopore detector according to any one of claims 5 to 7, characterized in that: The height of the mounting position is smaller than the thickness of the chip, so that when the locking mechanism is in a locked state, the first surface of the pressing member abuts against the first surface of the chip.

9. A nanopore detector according to any one of claims 4 to 8, characterized in that: The upper cover is provided with a first magnetic block, and the fixing plate is provided with a magnetic metal part that cooperates with the first magnetic block.

10. A nanopore detector according to any one of claims 1 to 8, characterized in that: The main control board is horizontally arranged on the connecting board.

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