Auxiliary device for microinjection

Abstract

The utility model discloses an auxiliary device for microinjection, which comprises an injection table serving as a mounting carrier, a cylinder bin is mounted in the center of the top of the injection table, six groups of screw rods are arranged in the injection table in a central symmetry manner, and inner threaded blocks are arranged on the outer sides of the screw rods in a threaded manner. An outer sleeve is mounted at the top of the internal thread block, and a fixing pipe is arranged in the outer sleeve. According to the device, the driving device is controlled to drive the six groups of screw rods to synchronously rotate, so that the internal thread blocks are forced to drive the outer sleeves to move towards the central position of the cylindrical bin, and a culture dish positioned in the cylindrical bin is jointly pushed to the central position in the cylindrical bin through one ends of the six groups of outer sleeves and is clamped and fixed; as the six groups of outer sleeves can freely and synchronously move, culture dishes with different sizes can be fixed and automatically pushed to the central position of the bottom in the cylindrical bin, so that the convenience of the microinjection equipment in the use process is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical instruments, in particular to an auxiliary device for microinjection. Background Art

[0002] Microinjection utilizes a glass microinjection needle with an extremely fine tip to directly inject exogenous gene fragments into pronuclear embryos or cultured cells. The exogenous gene is then integrated into the host chromosomes through potential recombination, deletion, duplication, or translocation of host genomic sequences. This microinjection procedure requires extremely sophisticated micromanipulation equipment. A micropipette extender is required to create a long tip, and a micromanipulator is required to secure the tip during injection. Therefore, auxiliary devices are often required to ensure effective microinjection.

[0003] Currently, during microinjection, the culture dish containing the cells to be injected often needs to be manually fixed. However, different culture dishes have different sizes, making it difficult to accurately fix the culture dish in the center of the injection platform, thereby reducing the convenience of using the microinjection equipment. At the same time, during the process of injecting cells in the culture dish, the position of the cells often deviates from the cell injection needle or cell holder needle. Manually moving the fixed culture dish is very inconvenient and cannot accurately adjust the rotation angle of the culture dish, thereby reducing the efficiency of microinjection. Utility Model Content

[0004] The purpose of the present utility model is to provide an auxiliary device for microinjection to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an auxiliary device for microinjection, comprising an injection platform as a mounting carrier, a cylindrical silo installed at the center position of the top of the injection platform, six groups of screw rods are symmetrically arranged at the inner center of the injection platform, the outer threads of the screw rods are provided with internal thread blocks, an outer sleeve is installed on the top of the internal thread block, a fixed tube is provided inside the outer sleeve, and one end of the fixed tube is fixedly connected to the top of the inner wall of the injection platform, a driving device for driving the six groups of screw rods to rotate is provided at the center position of the bottom of the cylindrical silo, a protective partition is installed at the end of the inner sleeve away from the edge of the injection platform, and the ends of the tops of the six groups of fixed tubes that are close to each other pass through limiting perforations and are commonly provided with a position adjustment component.

[0006] Preferably, the position adjustment component includes a mounting frame, and six groups of mounting frames are provided. The mounting frames are located at the end of the top of the outer sleeve away from the edge of the injection table, and guide rollers are provided inside the mounting frames. A second servo motor is installed at one end of the top of the outer sleeve close to the mounting frame, and synchronous wheels are installed on the output end of the second servo motor and the rotating shaft of an adjacent group of guide rollers, and a synchronous belt is commonly provided on the outer sides of the two groups of synchronous wheels.

[0007] Preferably, the driving device includes a first servo motor that provides driving force, the first servo motor is fixedly installed at the center position of the bottom of the cylindrical silo, the output end of the first servo motor is installed with a driving bevel gear, and the outer ends of the six groups of screw rods close to the first servo motor are all installed with driven bevel gears that mesh with the driving bevel gear.

[0008] Preferably, auxiliary bearings are symmetrically installed at both ends of the outer side of the screw rod, and the outer rings of the two sets of auxiliary bearings are fixedly connected to the inner bottom of the injection platform.

[0009] Preferably, a connecting bearing is installed at an edge position inside the cylindrical silo, and a rotating disk is installed on the inner ring of the connecting bearing.

[0010] Preferably, a square through hole is provided at one end of the outer sleeve near the center of the injection platform, and a stabilizing bearing is provided at one end of the top of the outer sleeve near the square through hole.

[0011] Preferably, the cross-sections of the outer sleeve and the fixed tube are both square structures, and the inner side of the outer sleeve is filled with lubricating oil.

[0012] Beneficial effects

[0013] Compared with the prior art, the present invention provides an auxiliary device for microinjection, which has the following beneficial effects:

[0014] 1. After the culture dish containing cells to be injected is placed inside the cylindrical silo, the utility model controls the driving device to drive the six sets of screws to rotate synchronously, and uses the action of the threads to force the internal thread block to drive the outer sleeve to move toward the center of the cylindrical silo. Then, one end of the six sets of outer sleeves jointly pushes the culture dish inside the cylindrical silo to the center of the cylindrical silo and clamps it. Since the six sets of outer sleeves can move freely and synchronously, culture dishes of different sizes can be fixed and automatically pushed to the center of the bottom of the cylindrical silo, thereby improving the convenience of using the microinjection device.

[0015] 2. After the cell culture dish is fixed at the center position inside the cylindrical silo, if the position of the cells in the culture dish needs to be adjusted, the second servo motor can be controlled to cooperate with the synchronous wheel and the synchronous belt to drive a group of guide rollers connected thereto to rotate. The cell culture dish is clamped and fixed at the center position inside the cylindrical silo by six groups of guide rollers. Therefore, when one group of guide rollers rotates, it will drive the cell culture dish between the six groups of guide rollers to rotate slowly, thereby achieving free and precise adjustment of the rotation angle of the cell culture dish without releasing the fixation of the cell culture dish, thereby achieving adjustment of the position between the cells in the cell culture dish and the cell injection needle or the cell holder needle, thereby improving the efficiency of microinjection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a top view of the utility model;

[0017] Figure 2 This is a front sectional view of the present utility model;

[0018] Figure 3 This is a three-dimensional schematic diagram of the cylindrical silo of the utility model;

[0019] Figure 4 This is a three-dimensional schematic diagram of the connection between the outer sleeve and the fixed pipe of the utility model;

[0020] Figure 5 For this utility model Figure 2 Enlarged view of point A.

[0021] In the picture:

[0022] 10. Injection table; 11. Screw; 12. Internal thread block; 13. Outer sleeve; 131. Square through hole; 14. Fixed tube; 15. Drive device; 151. First servo motor; 152. Driving bevel gear; 153. Driven bevel gear; 16. Protective partition; 17. Auxiliary bearing;

[0023] 20. Cylindrical silo; 21. Position limiting perforation; 22. Connecting bearing; 23. Rotating plate;

[0024] 30. Position adjustment assembly; 31. Mounting frame; 32. Guide roller; 33. Second servo motor; 34. Synchronous pulley; 35. Synchronous belt. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] like Figure 1-5 As shown, an auxiliary device for microinjection includes an injection platform 10 as a mounting carrier, a cylindrical silo 20 is installed at the center position of the top of the injection platform 10, six groups of screw rods 11 are symmetrically arranged inside the center of the injection platform 10, the outer thread of the screw rod 11 is provided with an internal thread block 12, an outer sleeve 13 is installed on the top of the internal thread block 12, a fixed tube 14 is provided inside the outer sleeve 13, and one end of the fixed tube 14 is fixedly connected to the top of the inner wall of the injection platform 10, a driving device 15 for driving the six groups of screw rods 11 to rotate is provided at the center position of the bottom of the cylindrical silo 20, a protective partition 16 is installed at the end of the outer sleeve 13 away from the edge of the injection platform 10, and the ends of the tops of the six groups of fixed tubes 14 that are close to each other pass through the limiting perforation 21 and are jointly provided with a position adjustment component 30.

[0027] In this embodiment, the position adjustment assembly 30 includes a mounting frame 31, and six groups of mounting frames 31 are provided. The mounting frames 31 are located at the end of the top of the outer sleeve 13 away from the edge of the injection table 10. A guide roller 32 is provided inside the mounting frame 31. A second servo motor 33 is installed at one end of the top of a group of outer sleeves 13 close to the mounting frame 31, and a synchronous wheel 34 is installed on the output end of the second servo motor 33 and the rotating shaft of an adjacent group of guide rollers 32. A synchronous belt 35 is commonly provided on the outside of the two groups of synchronous wheels 34.

[0028] In this embodiment, the driving device 15 includes a first servo motor 151 that provides driving force. The first servo motor 151 is fixedly installed at the center position of the bottom of the cylindrical silo 20. The output end of the first servo motor 151 is installed with a driving bevel gear 152. The outer ends of the six groups of screw rods 11 close to the first servo motor 151 are all installed with driven bevel gears 153 that are meshed with the driving bevel gear 152. The first servo motor 151 is controlled to drive the driving bevel gear 152 to rotate, and then the driving bevel gear 152 cooperates with the driven bevel gear 153 to drive the six groups of screw rods 11 to rotate synchronously.

[0029] In this embodiment, auxiliary bearings 17 are symmetrically installed at both ends of the outer side of the screw rod 11, and the outer rings of the two sets of auxiliary bearings 17 are fixedly connected to the inner bottom of the injection platform 10, which helps to improve the stability of the screw rod 11 during rotation.

[0030] In this embodiment, a connecting bearing 22 is installed at the edge of the cylindrical silo 20, and a rotating disk 23 is installed on the inner ring of the connecting bearing 22, which helps the rotating disk 23 to rotate along with the culture dish on the rotating disk 23, thereby improving the accuracy of the rotation angle of the culture dish.

[0031] In this embodiment, a square through hole 131 is opened at one end of a set of outer sleeves 13 near the center of the injection table 10, and a stabilizing bearing is provided at one end of the top of the outer sleeve 13 near the square through hole 131, which helps to allow the synchronous belt 35 to pass through the square through hole 131 and cooperate with the synchronous wheel 34 to drive the connected set of guide rollers 32 to rotate.

[0032] In this embodiment, the cross-sections of the outer sleeve 13 and the fixed tube 14 are both square structures, and the inner side of the outer sleeve 13 is filled with lubricating oil, which helps to improve the stability of the fixed tube 14 during the sliding process in the outer sleeve 13.

[0033] Working principle: Turn on the power before use, first place the culture dish containing cells on the top of the rotating disk 23 at the bottom of the cylindrical silo 20, then control the first servo motor 151 to drive the driving bevel gear 152 to rotate, and the driving bevel gear 152 cooperates with the driven bevel gear 153 to drive the six sets of screws 11 to rotate synchronously clockwise, and the thread action forces the internal thread block 12 to drive the outer sleeve 13 to move toward the center of the cylindrical silo 20. During this process, the fixed tube 14 slides inside the outer sleeve 13, and then the guide rollers 32 at one end of the six sets of outer sleeves 13 jointly push the culture dish to the top of the rotating disk 23. When the position between the cell and the cell injection needle or the cell holder needle needs to be adjusted during the microinjection operation, the second servo motor 33 is controlled to cooperate with the synchronous wheel 34 and the synchronous belt 35 to drive a group of guide rollers 32 connected thereto to rotate. The cell culture dish is clamped and fixed at the center position inside the cylindrical silo 20 by six groups of guide rollers 32. Therefore, when one group of guide rollers 32 rotates, it will drive the cell culture dish between the six groups of guide rollers 32 to rotate slowly, and rotate together with the rotating disk 23 at the bottom of the culture dish. At this time, the position of the cells in the culture dish also changes.

[0034] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0035] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An auxiliary device for microinjection, comprising an injection platform (10) as a mounting carrier, characterized in that: A cylindrical silo (20) is installed at the center position of the top of the injection platform (10), and six groups of screw rods (11) are symmetrically arranged inside the center of the injection platform (10). The outer threads of the screw rods (11) are provided with internal thread blocks (12), and the top of the internal thread blocks (12) is provided with an outer sleeve (13). A fixed tube (14) is provided inside the outer sleeve (13), and one end of the fixed tube (14) is fixedly connected to the top of the inner wall of the injection platform (10). A driving device (15) for driving the six groups of screw rods (11) to rotate is provided at the center position of the bottom of the cylindrical silo (20), and a protective partition (16) is installed at the end of the inner sleeve (13) away from the edge of the injection platform (10). The ends of the tops of the six groups of fixed tubes (14) that are close to each other pass through the limiting perforation (21) and are jointly provided with a position adjustment component (30).

2. The microinjection assisting device according to claim 1, characterized in that: The position adjustment assembly (30) includes a mounting frame (31), and six groups of the mounting frames (31) are provided. The mounting frames (31) are located at one end of the top of the outer sleeve (13) away from the edge of the injection platform (10). A guide roller (32) is provided inside the mounting frame (31). A second servo motor (33) is installed at one end of the top of the outer sleeve (13) close to the mounting frame (31), and a synchronous wheel (34) is installed on the output end of the second servo motor (33) and the rotating shaft of the adjacent group of guide rollers (32). A synchronous belt (35) is commonly provided on the outer sides of the two groups of synchronous wheels (34).

3. The microinjection assisting device according to claim 1, characterized in that: The driving device (15) comprises a first servo motor (151) for providing driving force, wherein the first servo motor (151) is fixedly mounted at the center of the bottom of the cylindrical silo (20), and a driving bevel gear (152) is mounted on the output end of the first servo motor (151). The six groups of screw rods (11) are all mounted on one end of the screw rods (11) close to the first servo motor (151) and meshed with the driving bevel gear (152).

4. The microinjection assisting device according to claim 1, characterized in that: Auxiliary bearings (17) are symmetrically installed at both ends of the outer side of the screw rod (11), and the outer rings of the two sets of auxiliary bearings (17) are fixedly connected to the inner bottom of the injection platform (10).

5. The microinjection assisting device according to claim 1, characterized in that: A connecting bearing (22) is installed at the edge of the cylindrical silo (20), and a rotating disk (23) is installed on the inner ring of the connecting bearing (22).

6. The microinjection assisting device according to claim 1, characterized in that: A square through hole (131) is provided at one end of the outer sleeve (13) near the center of the injection platform (10), and a stabilizing bearing is provided at one end of the top of the outer sleeve (13) near the square through hole (131).

7. The microinjection assisting device according to claim 1, characterized in that: The cross sections of the outer sleeve (13) and the fixed tube (14) are both square structures, and the inner side of the outer sleeve (13) is filled with lubricating oil.

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