Ultrasonic probe combined patch clamp device

Abstract

The utility model discloses an ultrasonic probe combined patch clamp device which comprises a placing table and a patch clamp bearing base, the patch clamp bearing base is arranged at the top of the placing table, and the patch clamp bearing base is used for placing a culture dish; the ultrasonic probe is arranged above the patch clamp bearing base; the regulation and control mechanism is connected with the ultrasonic probe and the placement table, and the regulation and control mechanism is used for regulating the position and the angle of the ultrasonic probe. An ultrasonic technology and a patch clamp technology can be combined by utilizing the patch clamp bearing base and the ultrasonic probe, so that the ultrasonic technology and the patch clamp technology can be used at the same time, operation steps during an experiment are reduced, the difficulty of experiment operation is greatly reduced, the ultrasonic technology and the patch clamp technology can be used in a combined manner, and the experiment efficiency is improved. More comprehensive cell and molecular biology research is carried out, and the occurrence mechanism of diseases is deeply understood.

Description

Technical Field

[0001] The utility model relates to the field of ultrasonic probes, in particular to an ultrasonic probe combined with a patch clamp device. Background Art

[0002] Ultrasound is a type of sound wave with a frequency above 20 kHz. It has excellent directionality and strong penetrating power, allowing for concentrated sound energy. It can travel long distances in water and is used for distance measurement, speed measurement, cleaning, welding, stone crushing, and sterilization. It has numerous applications in medicine, the military, industry, and agriculture. Ultrasound is named because its lower frequency limit is greater than the upper limit of human hearing. Under conditions of constant amplitude, the energy of an object's vibration is proportional to its frequency. When ultrasonic waves propagate through a medium, the frequency of the particles in the medium vibrates at a very high frequency.

[0003] By utilizing the biological effects of ultrasound, such as thermal effect, mechanical effect, cavitation effect, etc., and selecting appropriate ultrasound parameters, diseases can be diagnosed and treated. Patch clamp devices are often used to study the electrophysiological characteristics of cells. By recording the changes in current on the cell membrane, the functions and characteristics of ion channels can be understood in detail. In the existing technology, cell research requires the simultaneous use of ultrasonic instruments and patch clamp devices. Ultrasonic instruments and patch clamp devices are two instruments and equipment, which makes the operation of ultrasonic instruments and patch clamp devices more troublesome and cumbersome when used simultaneously, greatly increasing the difficulty of experimental operations. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the defect in the prior art that an ultrasonic instrument and a patch clamp device are difficult to combine, which affects the experimental operation, and provide an ultrasonic probe combined with a patch clamp device.

[0005] The utility model solves the above technical problems through the following technical solutions:

[0006] The utility model provides an ultrasonic probe combined with a patch clamp device, comprising a placement table,

[0007] A patch clamp supporting base, which is arranged on the top of the placement table and is used to place a culture dish;

[0008] An ultrasonic probe, the ultrasonic probe being arranged above the patch clamp supporting base;

[0009] A regulating mechanism, the regulating mechanism being connected to the ultrasound probe and the placement table respectively, and the regulating mechanism being used to adjust the position and angle of the ultrasound probe;

[0010] The regulating mechanism includes a rotating assembly and an angle control assembly. The rotating assembly enables the ultrasonic probe to rotate around the patch clamp supporting base, and the angle control assembly is used to adjust the tilt angle of the ultrasonic probe.

[0011] In this technical solution, the ultrasound technology and the patch clamp technology can be combined by using a patch clamp carrier base and an ultrasound probe, so that the ultrasound technology and the patch clamp technology can be used at the same time, reducing the steps of the experimental operation, thereby greatly reducing the difficulty of the experimental operation. The ultrasound technology and the patch clamp technology can be combined to conduct more comprehensive cell and molecular biology research and deeply understand the pathogenesis of the disease.

[0012] Preferably, the patch clamp supporting base includes a fixing seat connected to the top of the placement table, a holding groove is provided on the top surface of the fixing seat, and a plurality of clamping plates distributed in a circular array are provided inside the holding groove;

[0013] The side of the clamping plate close to the fixing seat is connected to a retractable air bag, and one side of the retractable air bag is connected to the wall of the receiving groove;

[0014] A retractable air path is provided in the fixing seat, one side of the retractable air path is connected to a plurality of retractable air bags, and one side of the retractable air path is connected to a charging and discharging port.

[0015] In this technical solution, the cell culture dish can be fixed using the patch clamp supporting base.

[0016] Preferably, a non-slip pad is connected to a side of the clamping plate away from the airbag.

[0017] In this technical solution, the use of a non-slip soft pad can increase the stability of the cell culture dish when it is fixed.

[0018] Preferably, the rotating assembly includes a protective outer frame, the protective outer frame is connected to the top of the placement table, and a rotating ring plate is provided at the inner cavity of the protective outer frame;

[0019] The bottom surface of the rotating ring plate is slidably connected to the rotating track ring, and the bottom of the rotating track ring is connected to the top of the placement table;

[0020] The side surface of the rotating ring plate is meshed with the rotating gear, the main body of the rotating gear is arranged outside the protective outer frame, the protective outer frame is connected to the power component, and the power component is installed in the inner cavity of the placement table.

[0021] In this technical solution, a rotating assembly is used to enable the ultrasonic probe to rotate around the patch clamp supporting base.

[0022] Preferably, the power assembly includes a bidirectional power source, which is installed in the inner cavity of the placement table, and both output ends of the bidirectional power source are connected to a transmission shaft;

[0023] The end of the transmission shaft away from the bidirectional power source is connected to bevel gear 1, and the side surface of bevel gear 1 is meshed with bevel gear 2. Bevel gear 2 is fixed to the surface of the connecting shaft, and the top of the connecting shaft is connected to the bottom of the rotating gear.

[0024] In this technical solution, the power component is used to provide driving force for the operation of the rotating component.

[0025] Preferably, the bottom end of the connecting shaft is rotatably connected to the bottom of the inner cavity of the placement table, and the top end of the connecting shaft is rotatably connected to the top surface of the placement table.

[0026] In this technical solution, the connecting shaft can be used to connect the bevel gear 2 and the rotating gear.

[0027] Preferably, the angle control assembly includes a support plate and a rotating shaft, and the bottom of the support plate is connected to the top of the rotating ring plate;

[0028] The surface of the rotating shaft is rotatably connected to the upper portion of the support plate, one end of the rotating shaft is connected to the rotating plate, and an ultrasonic probe is detachably mounted on one side of the rotating plate;

[0029] The other end of the rotating shaft is connected to a fixing plate, and the fixing plate is connected to the supporting plate through fixing bolts.

[0030] In this technical solution, the tilt angle of the ultrasound probe can be adjusted using the angle control component.

[0031] Preferably, a plurality of thread grooves distributed in a circular array are formed on one side of the support plate, and the fixing plate is threadedly connected to the fixing bolts.

[0032] In this technical solution, the threaded groove is used to enable the fixing bolt to be threadedly connected to the support plate.

[0033] Preferably, the support plate and the ultrasonic probe are connected via a support assembly, and the support assembly includes a triangular support frame;

[0034] One side of the triangular support frame is connected to one side of the support plate, and the other side of the triangular support frame is rotatably connected to a contact ball, and the contact ball contacts the top surface of the fixed seat;

[0035] The upper side of the triangular support frame is connected to a U-shaped frame, the inner side of the U-shaped frame is connected to an anti-deflection column, the anti-deflection column is slidably connected to a fixed track, and the fixed track is installed on the side of the U-shaped frame.

[0036] In this technical solution, the ultrasonic probe can be supported by the support assembly.

[0037] Preferably, a supporting ball is rotatably connected to the inner wall of the bottom surface of the U-shaped frame, and the supporting ball is in contact with the bottom surface of the ultrasound probe.

[0038] In this technical solution, the use of a supporting ball can make the ultrasonic probe move more smoothly.

[0039] On the basis of conforming to the common sense in this field, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present utility model.

[0040] The positive progress effect of this utility model is:

[0041] The utility model utilizes a patch clamp carrier base and an ultrasonic probe to combine ultrasound technology with patch clamp technology, thereby allowing ultrasound technology and patch clamp technology to be used simultaneously, reducing the number of steps in the experiment and greatly reducing the difficulty of the experiment operation. Thus, ultrasound technology and patch clamp technology can be combined to conduct more comprehensive cell and molecular biology research and gain a deeper understanding of the pathogenesis of the disease.

[0042] The position and tilt angle of the ultrasonic probe can be always adjusted by utilizing the rotating component and the angle control component, so that the ultrasonic probe can be moved arbitrarily and fit the periphery of the cell culture dish at multiple angles, which is convenient for the use of the ultrasonic probe. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is a schematic structural diagram of an ultrasonic probe combined with a patch clamp device according to an embodiment of the present invention.

[0044] Figure 2 for Figure 1 The figure shows a schematic diagram of the overall internal structure of the ultrasonic probe combined with the patch clamp device.

[0045] Figure 3 for Figure 1 The figure shows a side view of the angle control assembly of the ultrasonic probe combined with the patch clamp device.

[0046] Figure 4 for Figure 2 The diagram shows a locally enlarged structural diagram of the ultrasound probe combined with the patch clamp device at point A.

[0047] Description of Reference Numerals

[0048] 1. Placement table;

[0049] 2. Patch clamp base; 21. Fixed seat; 22. Clamping plate; 23. Retractable airbag; 24. Retractable air path; 25. Anti-slip cushion;

[0050] 3. Ultrasound probe;

[0051] 4. Rotating assembly; 41. Protective outer frame; 42. Rotating ring plate; 43. Track ring; 44. Rotating gear;

[0052] 5. Angle control assembly; 51. Support plate; 52. Rotation axis; 53. Rotation plate; 54. Fixed plate; 55. Fixing bolt;

[0053] 6. Power assembly; 61. Bidirectional power source; 62. Transmission shaft; 63. Bevel gear 1; 64. Bevel gear 2; 65. Connecting shaft;

[0054] 7. Support assembly; 71. Triangular support frame; 72. Contact ball; 73. U-shaped frame; 74. Anti-deflection column; 75. Fixed track; 76. Support ball;

[0055] 8. Patch clamp needle electrode. DETAILED DESCRIPTION

[0056] The present invention is further described below by way of examples, but the present invention is not limited to the scope of the examples.

[0057] Figures 1 to 4 The ultrasonic probe combined with the patch clamp device of the utility model is shown as a schematic diagram of the structure of the embodiment. The ultrasonic probe combined with the patch clamp device comprises a placement table 1,

[0058] A patch clamp supporting base 2, which is arranged on the top of the placement table 1 and is used to place a culture dish;

[0059] An ultrasonic probe 3, wherein the ultrasonic probe 3 is arranged above the patch clamp supporting base 2;

[0060] A regulating mechanism, which is connected to the ultrasound probe 3 and the placement table 1 respectively, and is used to adjust the position and angle of the ultrasound probe 3;

[0061] The regulating mechanism includes a rotating assembly 4 and an angle control assembly 5 . The rotating assembly 4 enables the ultrasonic probe 3 to rotate around the patch clamp supporting base 2 . The angle control assembly 5 is used to adjust the tilt angle of the ultrasonic probe 3 .

[0062] In this technical solution, the patch clamp base 2 and the ultrasonic probe 3 can be used to combine the ultrasonic technology and the patch clamp technology, so that the ultrasonic technology and the patch clamp technology can be used at the same time, reducing the steps of the experimental operation, thereby greatly reducing the difficulty of the experimental operation, so that the ultrasonic technology and the patch clamp technology can be combined to conduct more comprehensive cell and molecular biology research and deeply understand the pathogenesis of the disease.

[0063] The patch clamp supporting base 2 includes a fixing base 21, which is connected to the top of the placement platform 1. The top surface of the fixing base 21 is provided with a holding groove, and the inner side of the holding groove is provided with a plurality of clamping plates 22 distributed in a circular array.

[0064] The side of the clamping plate 22 close to the fixing seat 21 is connected to a retractable air bag 23, and one side of the retractable air bag 23 is connected to the wall of the receiving groove;

[0065] A retractable air path 24 is provided in the fixing seat 21 . One side of the retractable air path 24 is connected to the plurality of retractable air bags 23 . Another side of the retractable air path 24 is connected to a charging and discharging port.

[0066] In this technical solution, the patch clamp supporting base 2 can be used to fix the cell culture dish.

[0067] A non-slip cushion 25 is connected to a side of the clamping plate 22 away from the retractable airbag 23 .

[0068] In this technical solution, the anti-skid pad 25 can be used to increase the stability of the cell culture dish when it is fixed.

[0069] When in use, the cell culture dish is placed in the holding groove at the fixing seat 21, and then the retractable air path 24 is used to inflate the multiple retractable air bags 23 respectively, thereby driving the clamping plate 22 and the retractable air bags 23 to move, so that the retractable air bags 23 are close to the cell culture dish, thereby fixing the cell culture dish.

[0070] The rotating assembly 4 includes a protective outer frame 41, which is connected to the top of the placement table 1. A rotating ring plate 42 is provided in the inner cavity of the protective outer frame 41;

[0071] The bottom surface of the rotating ring plate 42 is slidably connected to the rotating track ring 43, and the bottom of the rotating track ring 43 is connected to the top of the placement table 1;

[0072] The side surface of the rotating ring plate 42 is meshed with the rotating gear 44 . The main body of the rotating gear 44 is arranged outside the protective outer frame 41 . The protective outer frame 41 is connected to the power component 6 . The power component 6 is installed in the inner cavity of the placement table 1 .

[0073] In this technical solution, the rotating assembly 4 is used to enable the ultrasonic probe 3 to rotate around the patch clamp supporting base 2.

[0074] During use, the power assembly 6 is used to drive the rotating gear 44 to rotate, thereby driving the rotating ring plate 42 to rotate, so that the rotating ring plate 42 rotates along the rotating track ring 43. When the rotating ring plate 42 rotates, it can drive the angle control assembly 5 to rotate, thereby driving the ultrasonic probe 3 to rotate. At this time, the ultrasonic probe 3 can be moved around the patch clamp supporting base 2, so that the ultrasonic probe 3 can be attached to the periphery of the cell culture dish at multiple angles.

[0075] The power assembly 6 includes a bidirectional power source 61, which is installed in the inner cavity of the placement table 1. Both output ends of the bidirectional power source 61 are connected to a transmission shaft 62;

[0076] The end of the transmission shaft 62 away from the bidirectional power source 61 is connected to a bevel gear 1 63 , and the side of the bevel gear 1 63 is meshedly connected to a bevel gear 2 64 . The bevel gear 2 64 is fixed to the surface of the connecting shaft 65 , and the top of the connecting shaft 65 is connected to the bottom of the rotating gear 44 .

[0077] In this technical solution, the power component 6 is used to provide driving force for the operation of the rotating component 4.

[0078] The bottom end of the connecting shaft 65 is rotatably connected to the bottom of the inner cavity of the placement table 1, and the top end of the connecting shaft 65 is rotatably connected to the top surface of the placement table 1.

[0079] In this technical solution, the connecting shaft 65 can be used to connect the second bevel gear 64 and the rotating gear 44.

[0080] When in use, the bidirectional power source 61 drives the transmission shaft 62 to rotate, thereby driving the bevel gear 1 63 to rotate, and then driving the bevel gear 2 64 to rotate. At this time, it can drive the connecting shaft 65 to rotate, thereby driving the rotating gear 44 to rotate, providing driving force for the rotation of the rotating gear 44.

[0081] The angle control assembly 5 includes a support plate 51 and a rotating shaft 52. The bottom of the support plate 51 is connected to the top of the rotating ring plate 42.

[0082] The surface of the rotating shaft 52 is rotatably connected to the upper portion of the support plate 51 . One end of the rotating shaft 52 is connected to a rotating plate 53 . An ultrasonic probe 3 is detachably mounted on one side of the rotating plate 53 .

[0083] The other end of the rotating shaft 52 is connected to a fixing plate 54 , and the fixing plate 54 is connected to the supporting plate 51 via fixing bolts 55 .

[0084] In this technical solution, the tilt angle of the ultrasonic probe 3 can be adjusted using the angle control component 5 .

[0085] A plurality of thread grooves distributed in a circular array are formed on one side of the support plate 51 , and the fixing plate 54 is threadedly connected to the fixing bolts 55 .

[0086] In this technical solution, the threaded groove is used to enable the fixing bolt 55 to be threadedly connected to the support plate 51 .

[0087] When in use, the fixed plate 54 can be used to rotate the rotating shaft 52, thereby driving the rotating plate 53 to rotate, thereby driving one end of the ultrasonic probe 3 to rotate, and the tilt angle of the ultrasonic probe 3 can be adjusted;

[0088] After the adjustment is completed, the fixing plate 54 and the support plate 51 can be connected by using the fixing bolts 55 , thereby limiting the angle of the fixing plate 54 and other structures, and further limiting the tilt angle of the ultrasonic probe 3 .

[0089] The support plate 51 and the ultrasonic probe 3 are connected via a support assembly 7, and the support assembly 7 includes a triangular support frame 71;

[0090] One side of the triangular support frame 71 is connected to one side of the support plate 51, and the other side of the triangular support frame 71 is rotatably connected to a contact ball 72, and the contact ball 72 contacts the top surface of the fixing seat 21;

[0091] A U-shaped frame 73 is connected to the upper side of the triangular support frame 71 , and an anti-deflection column 74 is connected to the inner side of the U-shaped frame 73 . The anti-deflection column 74 is slidably connected to a fixed rail 75 , and the fixed rail 75 is installed on the side of the U-shaped frame 73 .

[0092] In this technical solution, the ultrasonic probe 3 can be supported by the support assembly 7 .

[0093] The inner wall of the bottom surface of the U-shaped frame 73 is rotatably connected to a supporting ball 76 , and the supporting ball 76 is in contact with the bottom surface of the ultrasonic probe 3 .

[0094] In this technical solution, the use of the supporting ball 76 can make the ultrasonic probe 3 move more smoothly.

[0095] When the ultrasonic probe 3 rotates, the anti-deflection column 74 can slide in the fixed track 75, and the U-shaped frame 73 and the triangular support frame 71 can support the ultrasonic probe 3;

[0096] When the support plate 51 rotates, the triangular support frame 71 can be driven to rotate. At this time, the contact ball 72 can make the triangular support frame 71 rotate more smoothly.

[0097] During experimental operation, the ultrasonic probe 3 can irradiate the cell culture dish at multiple angles.

[0098] A patch clamp needle-like electrode 8 is installed on the top of the patch clamp supporting base 2, and a patch clamp microscope is set above the patch clamp supporting base 22. The patch clamp microscope is installed on the top of the placement table 1 through a bracket. The top of the patch clamp microscope is an objective lens for observing samples.

[0099] The movement of the patch clamp needle electrode 8 is controlled by a joystick of the patch clamp device itself.

[0100] The bidirectional power source 61 is a biaxial motor or other equipment that can output bidirectional rotational kinetic energy.

[0101] Although specific embodiments of the present invention have been described above, those skilled in the art will appreciate that these are merely illustrative and that the scope of protection of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, and such changes and modifications are intended to fall within the scope of protection of the present invention.

Claims

1. An ultrasonic probe combined with a patch clamp device, comprising a placement table (1), characterized in that: The ultrasonic probe combined with the patch clamp device further comprises: a patch clamp supporting base (2), the patch clamp supporting base (2) being arranged on the top of the placement table (1), and the patch clamp supporting base (2) being used for placing a culture dish; An ultrasonic probe (3), the ultrasonic probe (3) being arranged above the patch clamp supporting base (2); A regulating mechanism, the regulating mechanism being connected to the ultrasonic probe (3) and the placement table (1) respectively, and the regulating mechanism being used to adjust the position and angle of the ultrasonic probe (3); The regulating mechanism comprises a rotating assembly (4) and an angle control assembly (5); the rotating assembly (4) causes the ultrasonic probe (3) to rotate around the patch clamp supporting base (2); and the angle control assembly (5) is used to adjust the tilt angle of the ultrasonic probe (3).

2. The ultrasonic probe combined with the patch clamp device according to claim 1, wherein: The patch clamp supporting base (2) includes a fixing seat (21), the fixing seat (21) is connected to the top of the placement table (1), the top surface of the fixing seat (21) is provided with a receiving groove, and a plurality of clamping plates (22) distributed in a ring array are provided inside the receiving groove; The side of the clamping plate (22) close to the fixing seat (21) is connected to a retractable air bag (23), and one side of the retractable air bag (23) is connected to the wall of the receiving groove; A retractable air path (24) is provided in the fixing seat (21), one side of the retractable air path (24) is connected to a plurality of retractable air bags (23), and one side of the retractable air path (24) is connected to a charging and discharging port.

3. The ultrasonic probe combined with the patch clamp device according to claim 2, wherein: A non-slip cushion (25) is connected to the side of the clamping plate (22) away from the retractable airbag (23).

4. The ultrasonic probe combined with the patch clamp device according to claim 1, wherein: The rotating assembly (4) includes a protective outer frame (41), the protective outer frame (41) is connected to the top of the placement table (1), and a rotating ring plate (42) is provided at the inner cavity of the protective outer frame (41); The bottom surface of the rotating ring plate (42) is slidably connected to the rotating track ring (43), and the bottom of the rotating track ring (43) is connected to the top of the placement platform (1); The side surface of the rotating ring plate (42) is meshed with the rotating gear (44), the main body of the rotating gear (44) is arranged outside the protective outer frame (41), the protective outer frame (41) is connected to the power component (6), and the power component (6) is installed in the inner cavity of the placement table (1).

5. The ultrasonic probe combined with the patch clamp device according to claim 4, characterized in that: The power assembly (6) includes a bidirectional power source (61), which is installed in the inner cavity of the placement table (1), and both output ends of the bidirectional power source (61) are connected to a transmission shaft (62); One end of the transmission shaft (62) away from the bidirectional power source (61) is connected to a bevel gear 1 (63), and the side surface of the bevel gear 1 (63) is meshedly connected to a bevel gear 2 (64). The bevel gear 2 (64) is fixed to the surface of a connecting shaft (65), and the top end of the connecting shaft (65) is connected to the bottom of the rotating gear (44).

6. The ultrasonic probe combined with the patch clamp device according to claim 5, characterized in that: The bottom end of the connecting shaft (65) is rotatably connected to the bottom of the inner cavity of the placement table (1), and the top end of the connecting shaft (65) is rotatably connected to the top surface of the placement table (1).

7. The ultrasonic probe combined with the patch clamp device according to claim 1, wherein: The angle control assembly (5) comprises a support plate (51) and a rotating shaft (52), wherein the bottom of the support plate (51) is connected to the top of the rotating ring plate (42); The surface of the rotating shaft (52) is rotatably connected to the upper portion of the support plate (51), one end of the rotating shaft (52) is connected to a rotating plate (53), and an ultrasonic probe (3) is detachably mounted on one side of the rotating plate (53); The other end of the rotating shaft (52) is connected to a fixed disk (54), and the fixed disk (54) is connected to the support plate (51) via a fixing bolt (55).

8. The ultrasonic probe combined with the patch clamp device according to claim 7, characterized in that: A plurality of thread grooves distributed in a circular array are provided on one side of the support plate (51), and the fixing plate (54) is threadedly connected to the fixing bolt (55).

9. The ultrasonic probe combined with the patch clamp device according to claim 7, characterized in that: The support plate (51) and the ultrasonic probe (3) are connected via a support assembly (7), and the support assembly (7) includes a triangular support frame (71); One side of the triangular support frame (71) is connected to one side of the support plate (51), and the other side of the triangular support frame (71) is rotatably connected to a contact ball (72), and the contact ball (72) is in contact with the top surface of the fixing seat (21); The upper side of the triangular support frame (71) is connected to a U-shaped frame (73), the inner side of the U-shaped frame (73) is connected to an anti-deflection column (74), the anti-deflection column (74) is slidably connected to a fixed track (75), and the fixed track (75) is installed on the side of the U-shaped frame (73).

10. The ultrasonic probe combined with the patch clamp device according to claim 9, characterized in that: The inner wall of the bottom surface of the U-shaped frame (73) is rotatably connected to a supporting ball (76), and the supporting ball (76) is in contact with the bottom surface of the ultrasonic probe (3).

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