Salmon and trout embryo microinjection pretreatment device

Abstract

The utility model relates to the technical field of embryo micro-injection experimental devices, and discloses a salmon and trout embryo micro-injection pretreatment device which comprises an incubator, the placing assembly comprises a plurality of placing pieces and a plurality of connecting pieces, the connecting pieces are fixedly connected to the incubator in the axial direction, the placing pieces are placed in the incubator in the circumferential direction and detachably connected with the connecting pieces, and the placing pieces are used for storing salmon and trout roes; the temperature control part is arranged on the incubator and used for controlling the temperature in the incubator; and the oxygen control piece is arranged on the incubator and is used for controlling the oxygen content in the incubator. The device meets the requirements of constant low temperature and high oxygen in the operation process, is beneficial to improving the survival rate of subsequent embryo injection, can meet the experimental pretreatment requirements of multiple experimental batches, can effectively ensure the activity of roes before injection, and improves the injection efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of embryo microinjection experimental devices, in particular to a salmon and trout embryo microinjection pretreatment device. Background Art

[0002] Embryo microinjection in salmon and trout in the laboratory typically involves injection into one-cell embryos. Pretreatment involves egg retrieval, dry fertilization, egg washing, soaking in isotonic solution to prevent chorionic villi from hardening until the one-cell stage (approximately 2.5 hours), and injection. This complex and time-consuming process is also complex. Furthermore, due to the fragility of salmonid eggs, the experimenter must carefully manage operational details during the initial preparation, which increases the difficulty and affects injection efficiency. Currently, researchers often use buckets of isotonic solution to soak the eggs to complete this pretreatment process. Each experimental procedure can only complete the fertilization of one batch of embryos, which is time-consuming and labor-intensive. Temperature and dissolved oxygen levels are difficult to control, and time lags between samples can easily occur, which in turn affects the results of microinjection experiments. To address this issue, optimizing the experimental setup is necessary to improve experimental efficiency.

[0003] Therefore, there is an urgent need for a salmon and trout embryo microinjection pretreatment device to solve the above problems. Utility Model Content

[0004] The purpose of the utility model is to provide a salmon and trout embryo microinjection pretreatment device to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above-mentioned purpose, the present invention provides the following solution: The present invention provides a salmon and trout embryo microinjection pretreatment device, comprising:

[0006] incubator;

[0007] A placement assembly includes a plurality of placement members and a plurality of connecting members, wherein the plurality of connecting members are fixedly connected to the incubator along the axial direction, and the plurality of placement members are placed in the incubator along the circumferential direction and are detachably connected to the connecting members, and the placement members are used to store salmon and trout eggs;

[0008] a temperature control member, provided on the incubator, for controlling the temperature inside the incubator;

[0009] The oxygen control component is arranged on the incubator and is used to control the oxygen content in the incubator.

[0010] According to a salmon and trout embryo microinjection pretreatment device provided by the utility model, the placement component includes: a mesh groove, located in the incubator, the two ends of the mesh groove are respectively fixedly connected to one end of a first connecting rod, the other end of the first connecting rod is fixedly connected to one end of a hanging plate, and the other end of the hanging plate is hung on the wall of the incubator and is limitedly connected to the connecting component.

[0011] According to a salmon and trout embryo microinjection pretreatment device provided by the utility model, the connecting piece includes:

[0012] Two fixing blocks are fixedly connected to the tops of the two opposite outer side walls of the incubator, and the tops of the fixing blocks are provided with grooves, and one end of the hanging plate is adapted to the grooves;

[0013] The plug-in rod is slidably connected in the fixed block, and a connecting hole is provided at one end of the hanging plate extending into the groove, and the plug-in rod extends into the connecting hole.

[0014] According to a salmon and trout embryo microinjection pretreatment device provided by the utility model, a slide groove is opened on the outer side wall of the fixed block, a fixed rod is fixedly connected in the slide groove, a toggle block is slidably connected to the fixed rod, and the toggle block is fixedly connected to the plug rod through a second connecting rod.

[0015] According to the salmon and trout embryo microinjection pretreatment device provided by the utility model, a spring is sleeved on the fixing rod, and two ends of the spring are fixedly connected to the inner wall of the slide groove and the toggle block respectively.

[0016] According to the salmon and trout embryo microinjection pretreatment device provided by the utility model, the temperature control component includes: a temperature controller fixedly connected to the incubator, and the temperature in the incubator is adjusted within a range of 8°C-12°C.

[0017] According to the salmon and trout embryo microinjection pretreatment device provided by the utility model, the oxygen control component includes: an oxygen pump, the oxygen pump is fixedly connected to the incubator, and the dissolved oxygen value in the incubator is not less than 7 mg / L.

[0018] According to the salmon and trout embryo microinjection pretreatment device provided by the utility model, the mesh diameter of the net groove is 1 mm.

[0019] Compared with the prior art, the present invention has the following advantages and technical effects:

[0020] The utility model provides a salmon and trout embryo microinjection pretreatment device. Specifically, when in use, the temperature of the isotonic solution in the incubator is adjusted by a set temperature control part, and the oxygen content of the isotonic solution in the incubator is adjusted by a set oxygen control part. After the adjustment is completed, the placement piece storing salmon and trout eggs is installed on the incubator through a connecting piece, and the salmon and trout eggs are immersed in the isotonic solution. The injection is started when the eggs reach the one-cell stage. At the same time, the placement piece is easily disassembled by the provided connecting piece, and the eggs are taken frequently and less frequently during the injection process. At the same time, it can minimize the damage of external forces to salmonid fish eggs and improve the survival rate after injection. The present application meets the requirements of constant low temperature and high oxygen during the operation process, helps to improve the survival rate of subsequent embryo injections, can meet the experimental pretreatment requirements of multiple experimental batches, can effectively ensure the activity of the eggs before injection, and improve the injection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without inventive work:

[0022] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0023] Figure 2 For this utility model Figure 1 A partial enlarged view of the middle part;

[0024] Figure 3 This is a schematic diagram of the structure of the placement piece of the utility model;

[0025] Among them, 1. incubator; 2. net slot; 3. first connecting rod; 4. hanging plate; 5. fixing block; 6. groove; 7. plug-in rod; 8. connecting hole; 9. slide; 10. fixing rod; 11. toggle block; 12. second connecting rod; 13. spring; 14. temperature controller; 15. oxygen pump. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0028] Reference Figure 1-Figure 3 The utility model provides a salmon and trout embryo microinjection pretreatment device, comprising:

[0029] Incubator 1;

[0030] A placement assembly includes a plurality of placement members and a plurality of connecting members, wherein the plurality of connecting members are fixedly connected to the incubator 1 along the axial direction, and the plurality of placement members are placed in the incubator 1 along the circumferential direction and are detachably connected to the connecting members, and the placement members are used to store salmon and trout eggs;

[0031] A temperature control unit is provided on the incubator 1 and is used to control the temperature inside the incubator 1;

[0032] The oxygen control component is provided on the incubator 1 and is used to control the oxygen content in the incubator 1 .

[0033] In one embodiment of the present application, specifically, during use, the temperature of the isotonic solution in the incubator 1 is adjusted by the set temperature control component, and the oxygen content of the isotonic solution in the incubator 1 is adjusted by the set oxygen control component. After the adjustment is completed, the placement component storing salmon and trout eggs is installed on the incubator 1 through the connecting component, and the salmon and trout eggs are immersed in the isotonic solution. The injection is started when the eggs reach the one-cell stage. At the same time, the placement component is easily disassembled by the provided connecting component, and the eggs are taken frequently and sparingly during the injection process. At the same time, the damage to the salmonid eggs by external forces can be minimized, thereby improving the survival rate after injection.

[0034] As an optional embodiment, the placement piece includes: a mesh slot 2, located in the incubator 1, with one end of a first connecting rod 3 fixedly connected to both ends of the mesh slot 2, and the other end of the first connecting rod 3 fixedly connected to one end of a hanging plate 4, and the other end of the hanging plate 4 is hung on the wall of the incubator 1 and is limitedly connected to the connecting piece.

[0035] In one embodiment of the present application, salmon and trout eggs are stored in a mesh trough 2 and placed in an incubator 1, which is hung on the incubator 1 via a hanging plate 4, so that the salmon and trout eggs are immersed in an isotonic solution, and injection is started when the eggs reach the one-cell stage.

[0036] As an optional embodiment, the connecting member includes:

[0037] Two fixing blocks 5 are fixedly connected to the top of the two opposite outer walls of the incubator 1, and a groove 6 is opened on the top of the fixing block 5, and one end of the hanging plate 4 is adapted to the groove 6;

[0038] The plug-in rod 7 is slidably connected in the fixed block 5 , and a connecting hole 8 is opened at one end of the hanging plate 4 that extends into the groove 6 , and the plug-in rod 7 extends into the connecting hole 8 .

[0039] In one embodiment of the present application, when the hanging plate 4 is installed in the groove 6, the provided plug-in rod 7 is plugged into the connecting hole 8 to ensure a stable connection between the hanging plate 4 and the groove 6.

[0040] As an optional embodiment, a sliding groove 9 is opened on the outer wall of the fixed block 5, a fixed rod 10 is fixedly connected in the sliding groove 9, a toggle block 11 is slidably connected to the fixed rod 10, and the toggle block 11 is fixedly connected to the plug rod 7 through a second connecting rod 12.

[0041] In one embodiment of the present application, the plug rod 7 is moved by manually toggling the toggle block 11, thereby controlling the separation of the plug rod 7 from the hanging plate 4. After separation, the hanging plate 4 is manually lifted to take out the net slot 2 for use.

[0042] As an optional embodiment, a spring 13 is sleeved on the fixing rod 10 , and two ends of the spring 13 are fixedly connected to the inner wall of the sliding groove 9 and the toggle block 11 respectively.

[0043] In one embodiment of the present application, a spring 13 is provided to ensure a stable connection between the plug-in rod 7 and the hanging plate 4 .

[0044] Specifically, inclined surfaces can be provided on the bottom of the hanging plate 4 and the plug rod 7. Through the provided inclined surfaces, the plug rod 7 can be squeezed when the hanging plate 4 is installed. After subsequent installation, the plug rod 7 is reset to achieve connection.

[0045] As an optional embodiment, the temperature control component includes: a temperature controller 14, which is fixedly connected to the incubator 1, and the temperature in the incubator 1 is adjusted in the range of 8°C-12°C.

[0046] In one embodiment of the present application, the temperature of the culture medium inside the incubator 1 is controlled by a temperature controller 14 to ensure the survival rate of salmonid eggs. The specific specifications and models of the specific temperature controller 14 are selected by those skilled in the art according to actual needs and are not specifically limited here.

[0047] As an optional embodiment, the oxygen control component includes: an oxygen pump 15, which is fixedly connected to the incubator 1, and the dissolved oxygen value in the incubator 1 is not less than 7 mg / L.

[0048] In one embodiment of the present application, oxygen is supplied to the interior of the incubator 1 by an oxygen pump 15 .

[0049] As an optional embodiment, the mesh diameter of the net slot 2 is 1 mm. The specific specifications and model of the specific oxygen pump 15 are selected by those skilled in the art according to actual needs and are not specifically limited here.

[0050] In one embodiment of the present application, the mesh diameter of the mesh slot 2 is 1 mm to facilitate the storage of salmon and trout eggs.

[0051] Specifically, the mesh slot 2 is made of nylon or polyethylene, which can minimize the damage of external forces to salmonid eggs, thereby improving the survival rate after injection.

[0052] In the description of the present invention, it should be understood that the terms "longitudinal", "transverse", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they should not be understood as limitations on the present invention.

[0053] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements to the technical solutions of the present invention made by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A salmon and trout embryo microinjection pretreatment device, characterized in that: include: incubator(1); A placement assembly comprises a plurality of placement members and a plurality of connecting members, wherein the plurality of connecting members are fixedly connected to the incubator (1) along the axial direction, and the plurality of placement members are placed in the incubator (1) along the circumferential direction and are detachably connected to the connecting members, and the placement members are used to store salmon and trout eggs; A temperature control element, arranged on the incubator (1) and used to control the temperature inside the incubator (1); An oxygen control component is provided on the incubator (1) and is used to control the oxygen content in the incubator (1).

2. A salmon and trout embryo microinjection pretreatment device according to claim 1, characterized in that: The placement member comprises: a mesh slot (2) located in the incubator (1); the two ends of the mesh slot (2) are respectively fixedly connected to one end of a first connecting rod (3); the other end of the first connecting rod (3) is fixedly connected to one end of a hanging plate (4); the other end of the hanging plate (4) is hung on the wall of the incubator (1) and is limitedly connected to the connecting member.

3. A salmon and trout embryo microinjection pretreatment device according to claim 2, characterized in that: The connecting piece includes: Two fixing blocks (5) are respectively fixedly connected to the tops of the two opposite outer side walls of the incubator (1); a groove (6) is formed on the top of the fixing block (5); one end of the hanging plate (4) is adapted to fit in the groove (6); A plug-in rod (7) is slidably connected in the fixed block (5); a connecting hole (8) is provided at one end of the hanging plate (4) that extends into the groove (6); and the plug-in rod (7) extends into the connecting hole (8).

4. The salmon and trout embryo microinjection pretreatment device according to claim 3, characterized in that: A sliding groove (9) is provided on the outer wall of the fixed block (5), a fixed rod (10) is fixedly connected in the sliding groove (9), a toggle block (11) is slidably connected to the fixed rod (10), and the toggle block (11) is fixedly connected to the plug rod (7) via a second connecting rod (12).

5. The salmon and trout embryo microinjection pretreatment device according to claim 4, characterized in that: A spring (13) is sleeved on the fixing rod (10), and two ends of the spring (13) are fixedly connected to the inner wall of the sliding groove (9) and the toggle block (11) respectively.

6. The salmon and trout embryo microinjection pretreatment device according to claim 1, characterized in that: The temperature control component comprises a temperature controller (14) fixedly connected to the incubator (1), and the temperature in the incubator (1) is adjusted within a range of 8°C to 12°C.

7. The salmon and trout embryo microinjection pretreatment device according to claim 1, characterized in that: The oxygen control component comprises an oxygen pump (15), the oxygen pump (15) is fixedly connected to the incubator (1), and the dissolved oxygen value in the incubator (1) is not less than 7 mg / L.

8. The salmon and trout embryo microinjection pretreatment device according to claim 2, characterized in that: The mesh diameter of the mesh slot (2) is 1 mm.

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