Device for constructing COPD fruit fly model by adopting smoke exposure
By designing a smoke exposure device suitable for the COPD fruit fly model, the problem of lack of smoke exposure devices in the existing technology was solved, and efficient, safe and low-cost model construction and drug evaluation were achieved.
Patent Information
- Application Number
- CN202422920177.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-28
- Publication Date
- 2025-10-03
- Estimated Expiration
- 2034-11-28
AI Technical Summary
There is currently a lack of smoke exposure devices suitable for constructing COPD fruit fly models, which leads to problems such as low efficiency, poor safety and high cost in research.
A smoke exposure device was designed, which included a box body, a box cover, a sealing mechanism, a fruit fly placement mechanism, a smoke pushing mechanism, and a temperature and humidity detector. Through observation in the transparent box, uniform smoke distribution by a circulating fan, sealing by an elastic sleeve, and temperature and humidity control, uniform smoke distribution and experimental safety were ensured.
The construction of an efficient, safe and low-cost COPD fruit fly model has been achieved, which has improved experimental efficiency and success rate and provided technical support for drug treatment and efficacy evaluation.
Smart Images
Figure CN223403099U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to an auxiliary device for constructing an animal model, in particular to a device for constructing a COPD fruit fly model by using smoke exposure. Background Art
[0002] Drosophila melanogaster, a classic model animal, has been widely used in toxicology research. The entire Drosophila genome was essentially sequenced in the early 21st century, and nearly 75% of human disease genes have corresponding orthologs in Drosophila. Furthermore, Drosophila carries numerous characteristic chromosomes that facilitate genetic manipulation of phenotypic markers, molecular markers, or other characteristics. Due to its clear genetic background and ease of genetic manipulation, Drosophila has gained widespread application in fields such as developmental biology, biochemistry, molecular biology, and neuroscience. Compared to mammals, Drosophila offers advantages such as a short life cycle, ease of manipulation, high progeny numbers, and low research costs. Furthermore, Drosophila offers a variety of convenient genetic manipulation methods, allowing for targeted modulation of signaling pathways and fluorescent labeling of cells in vivo. The use of Drosophila melanogaster as a model for COPD has only recently begun to develop and offers many advantages. The Drosophila model facilitates the creation of fluorescently labeled trachea using the Gal4-UAS system, enabling more intuitive observation of early airway damage and ROS production. Due to the simple and clear genetic background of Drosophila, lacking redundant influences, the signaling pathways involved can be directly analyzed for their relevance to COPD through genetic or pharmacological methods. The Drosophila model allows for observation of the overall effects of drugs, making it suitable for evaluating and screening the activity of traditional Chinese medicine (TCM) compound formulas, single herbs, and their components, establishing a model system suitable for studying the mechanisms of action of TCM in COPD treatment.
[0003] In the construction of COPD models related to mice and rats, in order to successfully build the model, people have constructed modeling devices such as aerosol smoke poisoning exposure systems and animal fumigation modeling systems. However, for small animals, such as fruit flies, there is currently no complete set of smoke exposure equipment for constructing COPD fruit fly models. Utility Model Content
[0004] The purpose of the utility model is to provide a device for constructing a COPD fruit fly model by using smoke exposure, so as to fill the above-mentioned technical gap.
[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:
[0006] A device for constructing a COPD fruit fly model using smoke exposure, comprising a box body and a box cover, a sealing mechanism being provided between the box body and the box cover, the box body being a transparent structure, a fruit fly placement mechanism being provided inside the box body, a smoke inlet pipe being provided through the side wall of the box body, and a smoke pushing mechanism being provided on the smoke inlet pipe;
[0007] The fruit fly placement mechanism includes a tube rack and a plurality of fruit fly tubes vertically arranged in the tube rack, and a breathable plug body is provided at the open end of the fruit fly tube;
[0008] The smoke pushing mechanism includes a connecting tube and a smoke pushing cylinder. One end of the connecting tube is connected to the smoke pushing cylinder, and the other end is provided with a double-layer sleeve. The double-layer sleeve includes an outer sleeve and an inner sleeve arranged in the outer sleeve. The outer sleeve is sleeved on the outer periphery of the smoke inlet pipe, and the outer wall of the inner sleeve is tightly fitted with the inner wall of the smoke inlet pipe. The outer sleeve is connected to the outer wall of the connecting tube, and the inner sleeve is connected to the connecting tube. A valve body is passed through the connecting tube, and the smoke pushing cylinder includes a syringe and a piston passed through the syringe.
[0009] To further implement the present invention, an ash collection box is provided on the bottom of the box body, and is located below the end of the smoke inlet pipe. The ash collection box is filled with water in advance to collect and absorb the ashes from burned cigarettes to prevent them from floating in the box body.
[0010] In order to further realize the utility model, a circulation fan is provided in the box body, and the circulation fan and the smoke inlet pipe are arranged on the same side wall of the box body, and the circulation fan is arranged above the end of the smoke inlet pipe to evenly distribute the smoke in the box body.
[0011] In order to further realize the utility model, a temperature and humidity detector is provided in the box body for real-time monitoring of the temperature and humidity in the box body, so as to facilitate adjustment based on the situation.
[0012] To further realize the present invention, the fruit fly placement mechanism is arranged on a side away from the smoke inlet pipe. When the smoke concentration at the cigarette burning position is too high, the fruit flies are placed relatively away from this position and enter the fruit fly pipe after the circulating fan homogenizes the smoke.
[0013] To further implement the present invention, the sealing mechanism includes a sealing strip and adjustable buckles. The sealing strip is provided at the junction of the box lid and the box body, and a plurality of adjustable buckles are provided between the box lid and the box body. The adjustable buckles can be adjusted in tension to facilitate locking and sealing the gap between the box body and the box lid in conjunction with the sealing strip.
[0014] In order to further realize the present invention, the bottom surface of the box cover is further provided with a flange for engaging with the box body.
[0015] In order to further realize the present invention, the distance between the penetration end of the smoke inlet pipe and the top surface of the box body is 4 cm, and the diameter of the penetration end of the smoke inlet pipe is 1-1.5 cm.
[0016] In order to further realize the present invention, the outer sleeve and the inner sleeve are both made of elastic material, and a sealing ring is provided on the outer periphery of the end of the syringe.
[0017] In order to further realize the present invention, the axes of the smoke inlet pipe, the connecting pipe and the smoke pusher are on the same straight line, and the axis of the smoke inlet pipe overlaps with the horizontal axis of the box body.
[0018] Compared with the prior art, the present invention has the following beneficial effects:
[0019] The utility model can select a box body of suitable volume and a box cover matched therewith according to the experimental situation, which is convenient for calculating the concentration of smoke; the box body is a transparent structure, which is convenient for observing the progress of the experiment during the experiment; the tube rack can ensure that all fruit fly tubes are stably placed vertically, and the breathable plug body can be made of cotton, non-woven fabric, plug body with holes, etc., to ensure the entry of smoke and air; the outer shell and the inner sleeve are made of elastic materials such as rubber, elastic plastic, flexible resin, etc., and the outer shell is sleeved on the outside of the smoke inlet pipe to ensure that the connecting pipe and the smoke inlet pipe are sealed. The inner sleeve is used for installing cigarettes, that is, the suction mouth end of the cigarette is inserted into the inner sleeve, and it is stably clamped by the elastic effect of the inner sleeve, and the piston is slowly pushed into the syringe, and air is injected into the cigarette through the connecting pipe to help it burn. When the cigarette is burned out, the valve body is closed and the push-push pipe is removed.
[0020] In order to solve the shortcomings of the existing fruit fly COPD modeling device, the present invention is designed after a lot of exploration and trial, and research is conducted from multiple aspects such as the modeling device and the modeling method. The present invention is simple, efficient, practical, safe and low-cost.
[0021] The utility model facilitates the entry of smoke into the fumigation box through the smoke inlet for smoke exposure, can be used for the construction of COPD disease models and for drug treatment and efficacy evaluation, and provides technical support for the construction of COPD models through smoke exposure. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is a structural diagram of the utility model;
[0023] Figure 2 for Figure 1 A top view of
[0024] Figure 3 for Figure 2 AA sectional view in FIG (the chimney is not shown);
[0025] Figure 4 for Figure 2 BB cross-sectional view in (showing temperature and humidity detector);
[0026] Figure 5 for Figure 2 The CC cross-sectional view in Figure 1 (showing the circulation fan);
[0027] Figure 6It is a schematic diagram of the three-dimensional structure of the utility model;
[0028] Figure 7 for Figure 6 sectional view of
[0029] Figure 8 This is a schematic structural diagram of the smoke pushing mechanism in the present utility model;
[0030] The meanings of the accompanying figures are as follows: 1. Box body; 2. Box cover; 3. Sealing mechanism; 3-1. Sealing strip; 3-2. Adjustable buckle; 4. Fruit fly placement mechanism; 4-1. Pipe rack; 4-2. Fruit fly tube; 4-3. Ventilation plug; 5. Smoke inlet pipe; 6. Connecting pipe; 7. Smoke push tube; 7-1. Syringe; 7-2. Piston; 8. Outer shell; 9. Inner sleeve; 10. Valve body; 11. Ash collection box; 12. Circulation fan; 13. Temperature and humidity detector; 14. Flange; 15. Sealing ring; 16. Cigarette; 17. Power cord. DETAILED DESCRIPTION
[0031] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0032] like Figure 1-8 As shown, a device for constructing a COPD fruit fly model using smoke exposure is characterized by: comprising a box body 1 and a box cover 2, a sealing mechanism 3 is provided between the box body 1 and the box cover 2, the sealing mechanism 3 comprising a sealing strip 3-1 and an adjustable buckle 3-2, the sealing strip 3-1 being provided at the junction of the box cover 2 and the box body 1, a plurality of adjustable buckles 3-2 being provided between the box cover 2 and the box body 1, and a flange 14 being provided on the bottom surface of the box cover 2 for engaging with the box body 1; the box body 1 is a transparent structure, a fruit fly placement mechanism 4 is provided in the box body 1, a smoke inlet pipe 5 is provided through the side wall of the box body 1, the distance between the penetration end of the smoke inlet pipe 5 and the top surface of the box body 1 is 4 cm, and the penetration end of the smoke inlet pipe 5 has a diameter of 1-1.5 cm, a smoke pushing mechanism is provided on the smoke inlet pipe 5, and the fruit fly placement mechanism 4 is provided on the side away from the smoke inlet pipe 5;
[0033] The fruit fly placement mechanism 4 includes a tube rack 4-1 and a plurality of fruit fly tubes 4-2 vertically inserted into the tube rack 4-1. The open ends of the fruit fly tubes 4-2 are provided with air-permeable plugs 4-3.
[0034] The smoke pushing mechanism includes a connecting pipe 6 and a smoke pushing tube 7. One end of the connecting pipe 6 is connected to the smoke pushing tube 7, and the other end is provided with a double-layer sleeve. The double-layer sleeve includes an outer sleeve 8 and an inner sleeve 9 arranged in the outer sleeve 8. The outer sleeve 8 and the inner sleeve 9 are both made of elastic material. The outer sleeve 8 is sleeved on the outer periphery of the smoke inlet pipe 5, and the outer wall of the inner sleeve 9 is tightly fitted with the inner wall of the smoke inlet pipe 5. The outer sleeve 8 is connected to the outer wall of the connecting pipe 6, and the inner sleeve 9 is connected to the connecting pipe 6. A valve body 10 is passed through the connecting pipe 6. The smoke pushing tube 7 includes a syringe 7-1 and a piston 7-2 passed through the syringe 7-1. A sealing ring 15 is provided on the outer periphery of the end of the syringe 7-2. The axes of the smoke inlet pipe 5, the connecting pipe 6 and the smoke pushing tube 7 are on the same straight line, and the axis of the smoke inlet pipe 5 overlaps with the horizontal axis of the box body 1.
[0035] An ash collection box 11 is provided on the bottom surface of the box body 1, and the ash collection box 11 is arranged below the insertion end of the smoke inlet pipe 5. A circulation fan 12 is provided in the box body 1, and the circulation fan 12 and the smoke inlet pipe 5 are arranged on the same side wall of the box body 1. The circulation fan 12 is arranged above the insertion end of the smoke inlet pipe 5. A temperature and humidity detector 13 is provided in the box body 1.
[0036] Before modeling, the fruit fly eggs were transferred to the fruit fly tube 4-2 containing food and cultured for 4-5 days until they grew into third-instar larvae.
[0037] Insert the mouthpiece of the cigarette 16 into the open end of the inner sleeve 9, pull out the piston 7-2 to the end close to the rear end of the syringe 7-1, so that the syringe 7-1 is filled with air, and insert the end of the syringe 7-1 into the end of the connecting tube 6 away from the cigarette 16. The sealing ring 15 fits tightly with the inner wall of the connecting tube 6, and the valve body 10 is rotated to the open state to form the cigarette pushing mechanism.
[0038] The device of the present invention is placed in a fume hood with a temperature of 25°C and a humidity of 60%. The adjustable buckle 3-2 is opened to remove the box cover 2. The fruit fly tubes 4-2 filled with fruit flies are inserted one by one into the tube rack 4-1. The breathable plugs 4-3 are checked to see if they are properly covered. Water is poured into the ash collection box 11 so that the water surface at least covers the bottom of the box. The box cover 2 is inserted into the box body 1 through the flange 14. The box body 1 and the box cover 2 are tightened by the adjustable buckle 3-2. The joint between the two is sealed by the sealing strip 3-1. The power cord 17 of the circulation fan 12 is connected, and the circulation fan 12 is started.
[0039] During molding, immediately after lighting the cigarette 16, pass it through the smoke inlet pipe 5 into the box body 1, so that the inner sleeve 9 and the outer sleeve 8 are tightly fitted with the inner and outer walls of the smoke inlet pipe 5 respectively, observe the burning state of the cigarette 16, slowly push the piston 7-2 until the cigarette 16 is burned out, close the valve body 10, remove the smoke pusher 7, and make the smoke evenly fill the interior of the box body 1 under the action of the circulating fan 12, and observe the temperature and humidity detector 13 in real time during the molding process to ensure that the temperature and humidity remain basically constant.
[0040] The experimental results show that the length of the fruit fly trachea is shortened after exposure to cigarette smoke, and the genes in the inflammatory pathway are significantly upregulated, resulting in tracheal inflammation. The utility model can improve the efficiency and success rate of modeling, provide a basis for the clinical treatment of COPD and the development of related drugs, and facilitate the research and development of drug efficacy evaluation and screening.
Claims
1. A device for constructing a COPD fruit fly model using smoke exposure, characterized by: The invention comprises a box body (1) and a box cover (2), wherein a sealing mechanism (3) is provided between the box body (1) and the box cover (2), the box body (1) is a transparent structure, a fruit fly placement mechanism (4) is provided in the box body (1), a smoke inlet pipe (5) is passed through the side wall of the box body (1), and a smoke pushing mechanism is provided on the smoke inlet pipe (5); The fruit fly placement mechanism (4) comprises a tube rack (4-1) and a plurality of fruit fly tubes (4-2) vertically inserted into the tube rack (4-1), and a breathable plug (4-3) is provided at the open end of the fruit fly tube (4-2); The smoke pushing mechanism comprises a connecting tube (6) and a smoke pushing tube (7). One end of the connecting tube (6) is communicated with the smoke pushing tube (7), and the other end is provided with a double-layer sleeve. The double-layer sleeve comprises an outer sleeve (8) and an inner sleeve (9) arranged in the outer sleeve (8). The outer sleeve (8) is sleeved on the outer periphery of the smoke inlet tube (5). The outer wall of the inner sleeve (9) is tightly fitted with the inner wall of the smoke inlet tube (5). The outer sleeve (8) is connected to the outer wall of the connecting tube (6). The inner sleeve (9) is communicated with the connecting tube (6). A valve body (10) is passed through the connecting tube (6). The smoke pushing tube (7) comprises a syringe (7-1) and a piston (7-2) passed through the syringe (7-1).
2. The device for constructing a COPD fruit fly model using smoke exposure according to claim 1, characterized in that: A soot collection box (11) is provided on the bottom surface of the box body (1), and the soot collection box (11) is arranged below the penetration end of the smoke inlet pipe (5).
3. The device for constructing a COPD fruit fly model using smoke exposure according to claim 1 or 2, characterized in that: A circulation fan (12) is provided in the box body (1). The circulation fan (12) and the smoke inlet pipe (5) are arranged on the same side wall of the box body (1). The circulation fan (12) is arranged above the penetration end of the smoke inlet pipe (5).
4. The device for constructing a COPD fruit fly model using smoke exposure according to claim 3, wherein: A temperature and humidity detector (13) is provided in the box (1).
5. The device for constructing a COPD fruit fly model using smoke exposure according to claim 4, characterized in that: The fruit fly placement mechanism (4) is arranged on a side away from the smoke inlet pipe (5).
6. The device for constructing a COPD fruit fly model using smoke exposure according to claim 5, characterized in that: The sealing mechanism (3) comprises a sealing strip (3-1) and an adjustable buckle (3-2); the sealing strip (3-1) is arranged at the junction of the box cover (2) and the box body (1); and a plurality of adjustable buckles (3-2) are arranged between the box cover (2) and the box body (1).
7. The device for constructing a COPD fruit fly model using smoke exposure according to claim 6, wherein: The bottom surface of the box cover (2) is also provided with a flange (14) for engaging with the box body (1).
8. The device for constructing a COPD fruit fly model using smoke exposure according to claim 7, characterized in that: The distance between the penetration end of the smoke inlet pipe (5) and the top surface of the box body (1) is 4 cm, and the diameter of the penetration end of the smoke inlet pipe (5) is 1-1.5 cm.
9. The device for constructing a COPD fruit fly model using smoke exposure according to claim 8, characterized in that: The outer sleeve (8) and the inner sleeve (9) are both made of elastic material, and a sealing ring (15) is provided on the outer periphery of the end of the syringe (7-1).
10. The device for constructing a COPD fruit fly model using smoke exposure according to claim 9, characterized in that: The axes of the smoke inlet pipe (5), the connecting pipe (6) and the smoke pusher (7) are on the same straight line, and the axis of the smoke inlet pipe (5) overlaps with the horizontal axis of the box body (1).