Pipette tip with quadruple filtration
By designing a quadruple-filtered pipette tip, the problems of complexity and blockage and leakage in the liquid biological sample purification process were solved, achieving efficient and reliable sample purification results.
Patent Information
- Application Number
- CN202421981027.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-15
- Publication Date
- 2025-10-03
- Estimated Expiration
- 2034-08-15
AI Technical Summary
In the existing technology, the purification process of liquid biological samples is complicated, relies on specialized equipment, and is prone to protein clogging of filters or leakage of supernatant, making it difficult to automate and efficiently carry out.
A pipette tip with quadruple filtration is designed, including a connecting part, a filtering part and a head. Four filter beds are provided in the filtering part, namely the first filter bed, the second filter bed, the third filter bed and the fourth filter bed. The filter beds are made of polyethylene or ultra-high molecular weight polyethylene, and the pore size gradually decreases. Solid particles and filter membranes are provided to capture particles of different particle sizes to prevent blockage and leakage.
It achieves simple and efficient sample purification, reduces the risk of clogging, improves the reliability and consistency of the purification process, reduces operation time, and ensures that the filtered sample is pure and leak-free.
Smart Images
Figure CN223404961U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of purification of liquid biological samples, in particular to a pipette tip with quadruple filtration. Background Art
[0002] In the life sciences, purification of liquid biological samples is a critical step in diagnostic applications. The presence of impurities, such as proteins, can hinder accurate quantitative analysis and potentially impact diagnostic efficacy in clinical decision-making. Therefore, there is an urgent need for efficient and cost-effective sample purification methods.
[0003] Protein precipitation is a widely used, simple sample purification technique for removing proteins from liquid biological materials prior to LC-MS / MS analysis. It relies on precipitating proteins from the sample and then isolating the supernatant containing the target analyte. The protein precipitation process is performed in microcentrifuge tubes or 96-well plates. Once precipitation is complete, proteins can be removed by centrifugation or pressure-based filtration, and the supernatant is then used for analysis.
[0004] Reliance on specialized equipment such as vortexers, centrifuges, or pressure manifolds increases the complexity of the purification process. This added complexity makes it difficult to effectively automate the process. Each stage of the process requires multiple instruments and skilled personnel to operate them. Instrument complexity increases when high sample throughput is required. Existing technologies have addressed this issue by simplifying the process to filtration only. However, this also comes with its own disadvantages, such as proteins easily clogging the filter or the supernatant leaking from the filtration device during the transfer process.
[0005] Therefore, there is an urgent need to provide a pipette tip with quadruple filtration, which can achieve simple filtration and prevent protein from clogging the filter compared to the existing technology. Utility Model Content
[0006] The utility model solves the technical problems existing in the prior art and provides a pipette tip with quadruple filtration.
[0007] In order to achieve the above purpose, the technical solution adopted by the present utility model is as follows:
[0008] A pipette tip with quadruple filtration, comprising a connecting portion, a filtering portion, a head portion, and a filtering assembly, wherein one end of the filtering portion is integrally connected to the connecting portion, and the other end of the filtering portion is integrally connected to the head portion, the connecting portion, the filtering portion, and the head portion are sequentially connected, and the filtering assembly is disposed within the filtering portion;
[0009] The filter assembly includes a first filter bed, a second filter bed, a third filter bed, and a fourth filter bed, wherein the first filter bed, the second filter bed, the third filter bed, and the fourth filter bed are spaced apart along the axial direction of the filter portion.
[0010] Furthermore, the first filter bed is provided with a plurality of first filter holes, the second filter bed is provided with a plurality of second filter holes, the third filter bed is provided with a plurality of third filter holes, and the fourth filter bed is provided with a plurality of fourth filter holes;
[0011] The first filter bed, the second filter bed, the third filter bed and the fourth filter bed are all made of polyethylene or ultra-high molecular weight polyethylene.
[0012] Furthermore, the first filter hole is set to penetrate the first filter bed along the axial direction, the second filter hole is set to penetrate the second filter bed along the axial direction, the third filter hole is set to penetrate the third filter bed along the axial direction, and the fourth filter hole is set to penetrate the fourth filter bed along the axial direction.
[0013] Furthermore, the aperture of the second filter hole is the same as that of the third filter hole, the aperture of the first filter hole is larger than that of the second filter hole, and the aperture of the fourth filter hole is smaller than that of the second filter hole.
[0014] Furthermore, solid particles are arranged between the second filter bed and the third filter bed.
[0015] Furthermore, the particle size of the solid particles is larger than the pore size of the second filter pores.
[0016] Furthermore, a filter membrane is provided between the second filter bed and the third filter bed, and the material of the filter membrane is PTFE polytetrafluoroethylene or MCE mixed cellulose or PVDF polyvinylidene fluoride or Nylon nylon or PES polyethersulfone or CA cellulose acetate or GF glass fiber.
[0017] Furthermore, the pore size of the first filter pore is less than or equal to 20 μm, the pore size of the second filter pore and the third filter pore is less than 10 μm, and the pore size of the third filter pore is less than 5 μm.
[0018] Furthermore, the connecting portion is connected to a pipette gun or an automatic pipetting system.
[0019] Furthermore, the head is configured to be truncated cone-shaped, and is provided with a small diameter end and a large diameter end, the large diameter end is communicated with the filter portion, and the small diameter end is communicated with the outside of the head.
[0020] Compared with the prior art, the beneficial effects of the present invention are:
[0021] (1) The first filter bed, the second filter bed, the third filter bed and the fourth filter bed provided in the present invention are distributed in the filter part, which distributes the filter load, reduces the possibility of clogging, and ensures the consistency of performance during the sample purification process. At the same time, the filter part is provided with four filter beds, which can reduce the problem of supernatant leakage during loading or movement, and enhance the reliability of the purification process.
[0022] (2) The utility model has the advantages of simple filtering operation, high filtering efficiency, pure filtered samples, fast filtering speed, no clogging, and short operation time. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a structural diagram of embodiment 1 of the present utility model.
[0024] Figure 2 It is a structural diagram of embodiment 2 of the present utility model.
[0025] Description of reference numerals:
[0026] 1. Connecting part; 2. Filtering part; 21. First filter bed; 22. Second filter bed; 23. Third filter bed; 24. Fourth filter bed; 25. Solid particles; 26. Filter membrane; 3. Head. DETAILED DESCRIPTION
[0027] The technical solution of the present invention will be clearly described below in conjunction with the accompanying drawings. Obviously, the embodiments described are not all embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. It should be noted that the directions or positional relationships indicated by terms such as "center", "up", "down", "left", "right", "vertical", and "horizontal" are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the present invention.
[0028] Example 1
[0029] like Figure 1As shown, the utility model provides a pipette tip with quadruple filtration, including a connecting part 1, a filtering part 2, a head 3 and a filtering assembly, the upper end of the filtering part 2 is integrally connected to the connecting part 1, and the lower end of the filtering part 2 is integrally connected to the head 3, the connecting part 1, the filtering part 2, and the head 3 are connected in sequence, the connecting part 1 is connected to a pipette gun or an automatic pipetting system, and one end of the head 3 away from the filtering part 2 is connected to the outside thereof; the filtering part 2 is cylindrical, and the filtering part 2 and the head 3 are both truncated cone-shaped, the large diameter end of the filtering part 2 is connected to the connecting part 1, and the small diameter end of the filtering part 2 is connected to the large diameter end of the head 3; the filtering assembly is arranged inside the filtering part 2.
[0030] The filter assembly includes a first filter bed 21, a second filter bed 22, a third filter bed 23 and a fourth filter bed 24. The first filter bed 21, the second filter bed 22, the third filter bed 23 and the fourth filter bed 24 are arranged in sequence along the axial direction of the filter part 2. The first filter bed 21, the second filter bed 22, the third filter bed 23 and the fourth filter bed 24 are all cylindrical. The diameters of the first filter bed 21, the second filter bed 22, the third filter bed 23 and the fourth filter bed 24 gradually decrease. The first filter bed 21, the second filter bed 22, the third filter bed 23 and the fourth filter bed 24 are all fixed inside the filter part 2.
[0031] The first filter bed 21 is provided with a plurality of first filter holes, and each first filter hole is arranged to pass through the first filter bed 21 along the axial direction; the second filter bed 22 is provided with a plurality of second filter holes, and each second filter hole is arranged to pass through the second filter bed 22 along the axial direction; the third filter bed 23 is provided with a plurality of third filter holes, and each third filter hole is arranged to pass through the third filter bed 23 along the axial direction; the fourth filter bed 24 is provided with a plurality of fourth filter holes, and each fourth filter hole is arranged to pass through the fourth filter bed 24 along the axial direction; the pore size of the second filter hole is equal to the pore size of the third filter hole, the pore size of the first filter hole is larger than the pore size of the second filter hole or the third filter hole, and the pore size of the fourth filter hole is smaller than the pore size of the second filter hole or the third filter hole; the pore size of the first filter hole is less than or equal to 20μm, the pore size of the second filter hole and the third filter hole is less than 10μm, and the pore size of the fourth filter hole is less than 5μm.
[0032] The diameter range of the first filter bed 21 is 3.5-5.5 mm, the diameter range of the second filter bed 22 and the third filter bed 23 is 2.5-3.5 mm, and the diameter range of the fourth filter bed 24 is 1-2.5 mm; the first filter bed 21 is located at the upper end of the filter part 2, and the distance between the first filter bed 21 and the second filter bed 22, the distance between the second filter bed 22 and the third filter bed 23, and the distance between the third filter bed 23 and the fourth filter bed 24 can all be 5-20 mm.
[0033] The first filter bed 21, the second filter bed 22, the third filter bed 23 and the fourth filter bed 24 are all made of polyethylene or ultra-high molecular weight polyethylene. The first filter bed 21 is used to capture particles and pollutants in the sample whose particle size is greater than or equal to the aperture size of the first filter pore, the second filter bed 22 is used to capture particles and pollutants in the sample whose particle size is greater than or equal to the aperture size of the second filter pore, the third filter bed 23 is used to capture particles and pollutants in the sample whose particle size is greater than or equal to the aperture size of the third filter pore, and the fourth filter bed 24 is used to capture particles and pollutants in the sample whose particle size is greater than or equal to the aperture size of the fourth filter pore.
[0034] The working principle of a pipette tip with quadruple filtration provided in this embodiment is as follows: the sample is first precipitated and diluted, the processed sample is loaded into the pipette tip provided by the utility model, air is inhaled with a pipette to assist in sample loading and distribution, and the pipette tip provided by the utility model is then installed on a dedicated pipette. The sample is filtered sequentially through the first filter bed 21, the second filter bed 22, the third filter bed 23, and the fourth filter bed 24. After filtration and distribution, the filtered filtrate is directly injected into the mass spectrometer for further analysis.
[0035] The following describes the use of the pipette tip provided by the present invention based on a specific sample and a specific drug: the sample is serum and the drug is imatinib.
[0036] Step 1: Sample precipitation: 20 μL of serum sample was mixed with 40 μL of 100% methanol containing relevant internal standards in a 1:2 ratio and mixed thoroughly to ensure uniform distribution.
[0037] Step 2: Sample dilution: Dilute the precipitated sample into 180 μL of 50% methanol / water solution at a ratio of 1:3. Adjust the concentration in this step to make the sample suitable for analysis and adjust the concentration within the required range.
[0038] Step 3: Filtration and loading: 100 μL of the diluted sample is loaded into a special pipette equipped with the pipette tip provided by the utility model, and 400 μL of air is inhaled to facilitate sample loading and distribution.
[0039] Step 4: Distribution and filtrate collection. After loading, 100 μL of air was distributed at a rate of 500 μL / s. This process was repeated four times to ensure that all liquid contents were completely distributed. This distribution step took a total of about 10 s.
[0040] Step 5: Injection into the mass spectrometer. After filtration and partitioning, 2 μL of the filtrate is directly injected into the mass spectrometer for analysis. This process simplifies the steps and eliminates the need for further purification steps, thereby facilitating rapid analysis of therapeutic drug levels.
[0041] The pipette tip with four types of filtration provided by the utility model has simple filtration operation, high filtration efficiency, pure filtered samples, fast filtration speed, no clogging, and an operation time of 30s, which is a short operation time. At the same time, the first filter bed 21, the second filter bed 22, the third filter bed 23 and the fourth filter bed 24 are evenly distributed in the filter part 2, which distributes the filtration load, reduces the possibility of clogging, and ensures consistency performance during the sample purification process. At the same time, the filter part 2 is provided with four filter beds, which can reduce the problem of supernatant leakage during loading or moving, and enhance the reliability of the purification process.
[0042] Example 2
[0043] The difference between this embodiment and embodiment 1 is that: Figure 1 As shown, solid particles 25 are provided between the second filter bed 22 and the third filter bed 23. The particle size of the solid particles 25 is larger than the pore size of the second filter pore and the third filter pore. The solid particles 25 are carbon 8 or carbon 18 or HLB (polystyrene-divinylbenzene) or WAX (weak anion exchange) or WCX (weak cation exchange). The solid particles 25 are used to capture any particles that may escape during the initial filtration.
[0044] The working principle of a pipette tip with quadruple filtration provided in this embodiment is as follows: the sample is first precipitated and diluted, the processed sample is loaded into the pipette tip provided by the utility model, air is inhaled with a pipette to assist in sample loading and distribution, and the pipette tip provided by the utility model is then installed on a dedicated pipette. The sample is filtered sequentially through the first filter bed 21, the second filter bed 22, the solid particles 25, the third filter bed 23, and the fourth filter bed 24. After filtration and distribution, the filtered filtrate is directly injected into the mass spectrometer for further analysis.
[0045] Example 3
[0046] The difference between this embodiment and embodiment 1 is that: Figure 2 As shown, a filter membrane 26 is provided on the upper wall of the third filter bed 23. The filter membrane 26 contacts the upper wall of the third filter bed 23. The material of the filter membrane 26 is PTFE polytetrafluoroethylene or MCE mixed cellulose or PVDF polyvinylidene fluoride or Nylon nylon or PES polyethersulfone or CA cellulose acetate or GF glass fiber. The filter membrane 26 is used to capture any particles that may escape during the initial filtration.
[0047] The working principle of a pipette tip with quadruple filtration provided in this embodiment is as follows: the sample is first precipitated and diluted, the processed sample is loaded into the pipette tip provided by the utility model, air is inhaled with a pipette to assist in sample loading and distribution, and the pipette tip provided by the utility model is then installed on a dedicated pipette. The sample is filtered sequentially through the first filter bed 21, the second filter bed 22, the filter membrane 26, the third filter bed 23, and the fourth filter bed 24. After filtration and distribution, the filtered filtrate is directly injected into the mass spectrometer for further analysis.
[0048] Finally, it should be noted that the above content is only used to illustrate the technical solution of the utility model, rather than to limit the scope of protection of the utility model. Simple modifications or equivalent replacements of the technical solution of the utility model by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the utility model.
Claims
1. A pipette tip with quadruple filtration, characterized in that: The invention comprises a connecting portion, a filter portion, a head portion and a filter assembly, wherein one end of the filter portion is integrally connected to the connecting portion, and the other end of the filter portion is integrally connected to the head portion, the connecting portion, the filter portion and the head portion are sequentially connected, and the filter assembly is disposed in the filter portion; The filter assembly includes a first filter bed, a second filter bed, a third filter bed, and a fourth filter bed, wherein the first filter bed, the second filter bed, the third filter bed, and the fourth filter bed are spaced apart along the axial direction of the filter portion.
2. A pipette tip with quadruple filtration according to claim 1, characterized in that: The first filter bed is provided with a plurality of first filter holes, the second filter bed is provided with a plurality of second filter holes, the third filter bed is provided with a plurality of third filter holes, and the fourth filter bed is provided with a plurality of fourth filter holes; The first filter bed, the second filter bed, the third filter bed and the fourth filter bed are all made of polyethylene or ultra-high molecular weight polyethylene.
3. A pipette tip with quadruple filtration according to claim 2, characterized in that: The first filter hole is set to penetrate the first filter bed along the axial direction, the second filter hole is set to penetrate the second filter bed along the axial direction, the third filter hole is set to penetrate the third filter bed along the axial direction, and the fourth filter hole is set to penetrate the fourth filter bed along the axial direction.
4. A pipette tip with quadruple filtration according to claim 3, characterized in that: The aperture of the second filter hole is the same as that of the third filter hole, the aperture of the first filter hole is larger than that of the second filter hole, and the aperture of the fourth filter hole is smaller than that of the second filter hole.
5. A pipette tip with quadruple filtration according to claim 4, characterized in that: Solid particles are arranged between the second filter bed and the third filter bed.
6. A pipette tip with quadruple filtration according to claim 5, characterized in that: The particle size of the solid particles is larger than the pore size of the second filter pores.
7. A pipette tip with quadruple filtration according to claim 4, characterized in that: A filter membrane is provided between the second filter bed and the third filter bed. The filter membrane is made of polytetrafluoroethylene, mixed cellulose, polyvinylidene fluoride, nylon, polyethersulfone, cellulose acetate, or glass fiber.
8. A pipette tip with quadruple filtration according to claim 4, characterized in that: The pore size of the first filter pore is less than or equal to 20 μm, the pore size of the second filter pore and the third filter pore is less than 10 μm, and the pore size of the third filter pore is less than 5 μm.
9. A pipette tip with quadruple filtration according to claim 1, characterized in that: The connecting portion is connected to a pipette gun or an automatic pipetting system.
10. A pipette tip with quadruple filtration according to claim 1, characterized in that: The head is configured to be truncated cone-shaped and is provided with a small diameter end and a large diameter end. The large diameter end is communicated with the filter portion, and the small diameter end is communicated with the outside of the head.