Auxiliary device for realizing simultaneous single-photon and double-photon imaging of brain and spinal cord of mouse

Abstract

The utility model discloses an auxiliary device for achieving simultaneous single-photon and double-photon imaging of the brain and the spinal cord of a mouse, and relates to the technical field of experimental devices.The auxiliary device comprises a brain imaging auxiliary plate, a spine clamping piece and a spinal cord imaging auxiliary plate, a first water containing groove is formed in the middle of the brain imaging auxiliary plate, and a brain imaging auxiliary piece is fixed to the brain of the mouse in an adhesive mode; the spine clamping piece comprises a clamping bottom plate and a pair of clamping plate assemblies, and the clamping plate assemblies can get close to each other in the same direction and get away from each other in opposite directions to clamp and loosen the spine of the mouse; the spinal cord imaging auxiliary plate is detachably arranged on the clamping plate assembly, and a second water containing groove is formed in the middle of the spinal cord imaging auxiliary plate. The brain imaging plate is attached and fixed to the brain of the mouse, the spine clamping piece clamps the spine of the mouse, the spinal cord imaging auxiliary plate is fixed to the spine clamping piece, the spinal cord imaging auxiliary plate is attached to the spine of the mouse, the first water containing groove and the second water containing groove are used for containing water, and therefore groove milling in the skull and the spine of the mouse can be avoided; and the operation difficulty and the operation risk are greatly reduced.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of experimental devices, in particular to an auxiliary device for realizing single-photon and double-photon imaging of a mouse brain and a spinal cord at the same time. BACKGROUND

[0002] When single-photon and double-photon imaging of a mouse brain and a spinal cord is performed, a method currently adopted is that a milling cutter is used to mill grooves on the skull and the spine of the mouse, water is poured into the grooves, and then an imaging instrument is used to perform imaging.

[0003] The traditional operation mode has the defects of high risk coefficient and high operation difficulty. CONTENT OF THE INVENTION

[0004] The main purpose of the application is to provide an auxiliary device for realizing single-photon and double-photon imaging of a mouse brain and a spinal cord at the same time, and aims to solve the above problems.

[0005] The technical scheme adopted by the application is as follows:

[0006] An auxiliary device for realizing single-photon and double-photon imaging of a mouse brain and a spinal cord at the same time comprises:

[0007] A brain imaging auxiliary plate, which is centrally provided with a first water containing groove, and is fixedly connected to the mouse brain;

[0008] A spine clamping member, which comprises a clamping bottom plate and a pair of clamping plate assemblies, and the clamping plate assemblies can approach in the same direction and move away in the opposite direction to clamp and release the spine of the mouse;

[0009] A spinal cord imaging auxiliary plate, which is detachably arranged on the clamping plate assemblies, and is centrally provided with a second water containing groove.

[0010] Optionally, the clamping plate assemblies comprise:

[0011] An L-shaped sliding table, which is fixedly arranged on the clamping bottom plate, and the horizontal section of the L-shaped sliding table is provided with a sliding groove;

[0012] A sliding block, which is slidingly arranged in the sliding groove, and the sliding block is provided with a positioning bolt for locking the sliding block in the sliding groove;

[0013] A clamping block, which is arranged at the front end of the sliding block, and the end of the clamping block away from the sliding block is provided with a dovetail type clamping groove.

[0014] Optionally, the clamping block and the sliding block are detachably connected through a countersunk bolt.

[0015] Optionally, the spinal cord imaging auxiliary plate is detachably connected with the sliding block through a fastening bolt.

[0016] Optionally, the spinal cord imaging auxiliary plate is provided with a strip-shaped groove on both sides of the second water tank, and the fastening bolt can move in the strip-shaped groove.

[0017] Compared with the prior art, the beneficial effects of the application are:

[0018] The auxiliary device for realizing simultaneous single-photon and double-photon imaging of mouse brain and spinal cord provided by the embodiment of the application comprises a brain imaging auxiliary plate, a spinal column clamping piece and a spinal cord imaging auxiliary plate, the brain imaging plate is fixedly attached to the mouse brain, the spinal column clamping piece clamps the spinal column of the mouse, and the spinal cord imaging auxiliary plate is fixed on the spinal column clamping piece and attached to the spinal column of the mouse, water is contained in the first water tank and the second water tank, and thus the milling groove on the skull and the spinal column of the mouse can be avoided, and the operation difficulty and the operation risk are greatly reduced. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The structural schematic diagram of the auxiliary device for realizing simultaneous single-photon and double-photon imaging of mouse brain and spinal cord provided by the embodiment of the application is shown in one perspective view;

[0020] Figure 2 The structural schematic diagram of the auxiliary device for realizing simultaneous single-photon and double-photon imaging of mouse brain and spinal cord provided by the embodiment of the application is shown in another perspective view;

[0021] Figure 3 The structural schematic diagram of the auxiliary device for realizing simultaneous single-photon and double-photon imaging of mouse brain and spinal cord provided by the embodiment of the application is shown in another perspective view; Figure 2 The enlarged view of A in the middle.

[0022] Label explanation in the drawings:

[0023] 1-brain imaging auxiliary plate, 101-first water tank, 2-spinal column clamping piece, 201-clamping bottom plate, 202-L-shaped sliding table, 203-sliding block, 204-positioning bolt, 205-clamping block, 206-counter sunk bolt, 3-spinal cord imaging auxiliary plate, 301-second water tank, 302-strip-shaped groove, 4-fastening bolt. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the application will be clearly and completely described below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the application.

[0025] It should be noted that all the direction indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present application are only used to explain the relative position relationship, movement condition, etc. between components in a certain posture (as shown in the drawings), and if the certain posture changes, the direction indications will also change accordingly.

[0026] In the present application, unless specifically defined and limited otherwise, the terms "connection", "fixation" and the like should be understood in a broad sense, for example, "fixation" can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be direct connection, or indirect connection through an intermediate medium; can be internal connection of two elements or interaction relationship between two elements, unless otherwise specifically limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0027] In addition, if the present application has a description of "first", "second" and the like, the description of "first", "second" and the like is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing throughout the text includes three parallel solutions. For example, "A and / or B" includes A solution, or B solution, or A and B solution. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of ordinary skilled in the art, when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, nor within the scope of protection claimed in the present application.

[0028] Referring to the drawings Figures 1 to 3 The embodiment of the present application provides an auxiliary device for realizing simultaneous single-photon and two-photon imaging of mouse brain and spinal cord, which comprises a brain imaging auxiliary plate 1, a spinal column clamping piece 2 and a spinal cord imaging auxiliary plate 3. The brain imaging auxiliary plate 1 is a square strip-shaped plate, a first water tank 101 is arranged in the middle of the brain imaging auxiliary plate 1, and U-shaped sockets are arranged on both sides of the brain imaging auxiliary plate 1. The U-shaped sockets are used to fix the brain imaging auxiliary plate 1 on the base buckle of the imaging instrument during imaging. The brain imaging auxiliary piece is fixed to the mouse brain by structural adhesive, and the first water tank 101 and the surrounding part of the mouse brain are sealed by using structural adhesive, so as to avoid water seepage.

[0029] As shown in FIG. 1 Figures 1 to 3As shown, the spinal column clamping member 2 comprises a clamping base plate 201 and a pair of clamping plate assemblies, which are symmetrically arranged on the left and right of the center of the clamping base plate 201, and the clamping plate assemblies on both sides can be moved towards each other and away from each other to clamp and release the mouse spinal column. Specifically, the clamping plate assembly comprises an L-shaped sliding table 202, a sliding block 203 and a clamping block 205, wherein the vertical section of the L-shaped sliding table 202 is integrally formed on the clamping base plate 201, the horizontal section of the L-shaped sliding table 202 is separated from the clamping base plate 201 and is parallel to the clamping base plate 201, and the horizontal section of the L-shaped sliding table 202 is provided with a sliding groove in the direction of the long axis of the clamping base plate 201, the sliding block 203 is slidingly installed in the sliding groove, and the sliding block 203 is provided with a positioning bolt 204, which is used to lock the sliding block 203 at any position of the sliding groove. The clamping block 205 is arranged at one end of the sliding block 203 close to the center of the clamping base plate 201, and the clamping block 205 is detachably connected with the sliding block 203 through a countersunk head bolt 206, so as to replace the used clamping block 205. At the same time, the end of the clamping block 205 away from the sliding block 203 is designed with a dovetail type clamping groove, so as to clamp the mouse spinal column.

[0030] The above-mentioned Figures 1 to 3 As shown, the spinal cord imaging auxiliary plate 3 is a square plate, and the spinal cord imaging auxiliary plate 3 is arranged in the center of the second water tank 301, and the spinal cord imaging auxiliary plate 3 is detachably connected with the clamping block 205. Specifically, the spinal cord imaging auxiliary plate 3 is provided with a strip-shaped groove 302 on both sides of the second water tank 301, the strip-shaped groove 302 is provided with a fastening bolt 4, and the clamping block 205 is provided with a bolt hole for connecting the fastening bolt 4. After the spinal cord imaging plate is connected with the clamping block 205 through the fastening bolt 4, the strip-shaped groove 302 can provide convenience for the clamping and movement of the clamping block 205.

[0031] In summary, the auxiliary device for realizing single-photon and double-photon imaging of mouse brain and spinal cord at the same time provided by the embodiment of the present application has the following advantages:

[0032] First, the mouse is anesthetized on a sterile operation experiment table and the back and brain subcutaneous tissue is cut to expose the skull and spine; then the mouse spine is slightly raised, the clamping bottom plate 201 is inserted under the spine, the clamping plate assembly is located on both sides of the spine, the two side sliders 203 are moved to clamp the spine with the clamping block 205, and the positioning bolt 204 is locked, then one side of the spinal cord imaging auxiliary plate 3 is coated with structural adhesive and attached to the clamping block 205, the spinal cord imaging auxiliary plate 3 is installed on the clamping block 205 using the fastening bolt 4, the spinal cord imaging auxiliary plate 3 is attached to the mouse spine using structural adhesive to prevent water from seeping into the second water tank 301; then the brain imaging auxiliary plate 1 is attached to the mouse head using structural adhesive, the first water tank 101 is attached to the mouse head, and the gap between the skull and the brain imaging auxiliary plate 1 is sealed using structural adhesive to prevent water from seeping into the first water tank 101; finally, after the brain imaging auxiliary plate 1 and the spinal cord imaging auxiliary plate 3 are installed, the mouse is placed on the base of the imaging instrument, water is poured into the first water tank 101 and the second water tank 301, and imaging can be observed under the imaging instrument.

[0033] It can be seen that the auxiliary device for realizing single-photon and two-photon imaging of mouse brain and spinal cord provided by the embodiment of the application can avoid milling grooves on the mouse skull and spine by setting the brain imaging auxiliary plate, the spine clamping piece and the spinal cord imaging auxiliary plate, attaching and fixing the brain imaging plate to the mouse brain, clamping the mouse spine with the spine clamping piece, and fixing the spinal cord imaging auxiliary plate on the spine clamping piece, and attaching the spinal cord imaging auxiliary plate to the mouse spine, and pouring water into the first water tank and the second water tank, thereby greatly reducing the operation difficulty and operation risk.

[0034] The above only describes the preferred embodiments of the application and is not intended to limit the application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the application shall be included in the protection scope of the application.

Claims

1. An auxiliary device for realizing simultaneous single and two-photon imaging of mouse brain and spinal cord, characterized in that, The utility model relates to a brain imaging auxiliary plate, a spinal column clamping piece and a spinal cord imaging auxiliary plate, and belongs to the field of medical imaging. The brain imaging auxiliary plate is centrally provided with a first water containing groove, and the brain imaging auxiliary piece is adhesively fixed to a mouse brain. The spinal column clamping piece comprises a clamping bottom plate and a pair of clamping plate assemblies, and the clamping plate assemblies can be moved towards each other and away from each other to clamp and release a mouse spinal column. The spinal cord imaging auxiliary plate is detachably arranged on the clamping plate assemblies, and the spinal cord imaging auxiliary plate is centrally provided with a second water containing groove.

2. The auxiliary device for realizing simultaneous single and two-photon imaging of mouse brain and spinal cord according to claim 1, characterized in that, The clamping plate assembly comprises an L-shaped sliding table, a sliding block and a clamping block. The L-shaped sliding table is fixedly arranged on the clamping bottom plate, and the horizontal section of the L-shaped sliding table is provided with a sliding groove. The sliding block is slidingly arranged in the sliding groove, and the sliding block is provided with a positioning bolt for locking the sliding block in the sliding groove. The clamping block is arranged at the front end of the sliding block, and the end of the clamping block away from the sliding block is provided with a dovetail type clamping groove.

3. The auxiliary device for realizing simultaneous single and two-photon imaging of mouse brain and spinal cord according to claim 2, characterized in that, The clamping block and the sliding block are detachably connected through a countersunk head bolt.

4. The auxiliary device for realizing simultaneous single and two-photon imaging of mouse brain and spinal cord according to claim 2, characterized in that, The spinal cord imaging auxiliary plate is detachably connected with the sliding block through a fastening bolt.

5. The auxiliary device for realizing simultaneous single and two-photon imaging of mouse brain and spinal cord according to claim 4, characterized in that, The spinal cord imaging auxiliary plate is provided with a strip-shaped groove on both sides of the second water containing groove, and the fastening bolt can move in the strip-shaped groove.

Images